TOTAL PHENOL AND ANTIOXIDANT ACTIVITY OF BROWN SEAWEED EXTRACT (Sargassum sp.)

Nirwana Beach is one of the tourist destinations in Padang West Sumatra, but almost every day littered by brown seaweed that washed up along the coast. Very unfortunate, the people around still consider this waste and do not know the usefulness and benefits. Brown seaweed has been widely studied. The content consist of alginate, fukosantin and secondary metabolites namely fucoidan. Fucoidan has been made into a supplement as antioxidant, anticoagulant and antithrombotic, antiviral, anticancer, antidiabetic, immunomodulating, antiinflammatory, antilipidemic and antifertilization. The purpose of the study was to determine the antioxidant activity of polyphenol extract from brown seaweed. The brown seaweed was extracted using aquades by heating and without heating, then the extract was evaporated with a rotary evaporator up to a volume of 50 ml extract and then analyzed the total phenol and its antioxidant activity test. The results showed extraction with heating had higher total phenol content than without heating. The total phenol of brown seaweed extract with heating is 669.33 mg galic acid/g, without heating 352.5 mg of gallic acid/g and on seaweed 538.5 mg gallic acid/g. The antioxidant activity of brown seaweed extract is very strong ie 4 ug/ml with heating and 16.6 ug/ml without heating.

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UJI AKTIVITAS ANTIOKSIDAN DAN PENENTUAN TOTAL KANDUNGAN FENOLIK EKSTRAK ETANOL DAUN NANAMUHA (Bridelia monoica Merr)

PHARMACON ◽

10.35799/pha.9.2020.29268 ◽

2020 ◽

Vol 9 (2) ◽

pp. 178

Author(s):

Julia Elsa Lakoro ◽

Max R. J. Runtuwene ◽

Paulina V. Y. Yamlean

Keyword(s):

Antioxidant Activity ◽

Extraction Method ◽

Total Phenol ◽

Total Phenolic ◽

Total Phenol Content ◽

Phenol Compounds ◽

Activity Test ◽

Ic50 Value ◽

Dpph Method ◽

Ethanol Extracts

ABSTRACTNanamuha (Bridelia monoica Merr.) is a plant that was used as a cancer and tumor drug by the Sangihe Islands community. This plant contains phenol compounds, flavonoids, alkaloids and tannins. The purpose of this study was to determine the potential of nanamuha leaves that grow in the Sangihe Island to have antioxidant activity and determine the total phenolic ethanol extracts of nanamuha leaf. The extraction method used is maceration with ethanol solvent p.a. The extracts obtained was determined total phenolic and antioxidant activity test using DPPH method. Based on the results obtained, the ethanol extracts of nanamuha leaves has antioxidant activity with an IC50 value of 75,03 µg / mL and the result showed that the total phenol content the ethanol extracts of nanamuha leaves of 53,34 mg / L. Keywords : Nanamuha leaf, total phenol, antioxidant activity, DPPH ABSTRAKNanamuha (Bridelia monoica Merr.) merupakan tanaman yang dimanfaatkan sebagai obat kanker dan tumor oleh masyarakat Kepulauan Sangihe. Tanaman ini memiliki kandungan senyawa fenol, flavonoid, alkaloid dan tanin. Tujuan dari penelitian ini adalah unutuk mengetahui potensi dari daun nanamuha yang tumbuh di Kepulaun Sangihe ini memiliki aktivitas antioksidan dan menentukan total fenolik dari ekstrak etanol daun nanamuha. Metode ekstraksi yang digunakan adalah maserasi dengan pelarut etanol p.a. Ekstrak yang diperoleh ditentukan total fenolik dan uji aktivitas antioksidan dengan metode DPPH. Berdasarkan hasil yang didapatkan ekstrak etanol daun nanamuha memiliki aktivitas antioksidan dengan nilai IC50 75,03 µg/mL dan hasil penelitian menunjukan total kandungan fenolik ekstrak etanol daun nanamuha sebesar 53,34 mg/L. Kata kunci : Daun Nanamuha, Total fenol, Aktivitas antioksidan, DPPH

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Uji Aktivitas Antioksidan dari Ekstak Etanol Daun Nipah (Nypa fruticans Wurmb.) dengan Metode DPPH

Talenta Conference Series Agricultural and Natural Resources (ANR) ◽

10.32734/anr.v1i2.238 ◽

2018 ◽

Vol 1 (2) ◽

pp. 205-212

Author(s):

Riza Silvia Anggraini ◽

Sudarmi Sudarmi ◽

Herawaty Ginting

Keyword(s):

Antioxidant Activity ◽

Gallic Acid ◽

Colorimetric Method ◽

Ethanol Extract ◽

Total Phenol ◽

Total Flavonoid ◽

Total Phenol Content ◽

Flavonoid Content ◽

Nypa Fruticans ◽

Gallic Acid Equivalent

Tumbuhan Nipah (Nypa fruticans Wurmb.) merupakan tumbuhan suku Arecaceae yang tumbuh di lingkungan hutan yang bermanfaatsebagai obat sakit perut, diabetes dan obat penurun panas . Penelitian ini bertujuan untuk mengetahui aktifitas antioksidan serta total fenol dan total flavonoid dari Ekstrak Etanol Daun Nipah (Nypa fruticans Wurmb.). Penelitian ini meliputi penyiapan sampel, pemeriksaan karakteristik simplisia, pembuatan ekstrak dengan metode maserasi, dan pengujian aktivitas antioksidan dari ekstrak etanol daun nipah dengan metode perangkapan radikal bebas menggunakan DPPH (1,1- difenil-2-pikrilhidrazil), pada panjang gelombang 515,50 nm, dibandingkan dengan vitamin C sebagai blanko positif, serta pengujian nilai kandungan total fenol ekstrak etanol daun nipah setara asam galat (Gallic Acid Equivalent (GAE)) dengan metode kolorimetri menggunakan reagen Folin-Ciocalteu pada panjang gelombang 775 nm dan pengukuran nilai kandungan total flavonoid ekstrak etanol daun nipah setara kuersetin (Quercetin Equivalent (QE)) dengan metode kolorimetri menggunkan aluminium klorida pada panjang gelombang 436,50 nm dengan menggunakan spektrofotometri UV-Visibel. Dari penelitian yang dilakukan diperoleh hasil uji aktivitas antioksidan yang menunjukkan bahwa ekstrak etanol daun nipah mempunyai aktivitas antioksidan yang sangat kuat dengan nilai IC50 sebesar 9,662 ppm serta nilai kandungan total fenol sebesar 85,51 mg/g GAE ekstrak dan nilai kandungan total flavonoid sebesar 12,49 mg/g QE ekstrak. Nipah plant (Nypa fruticans Wurmb.) Is a plant of the Arecaceae family that grows in a forest environment that is useful as a medicine for stomach pain, diabetes and febrifuge. This study aims to determine antioxidant activity, total phenol and total flavonoids from the Ethanol Extract of Nipah Leaves (N. fruticans). This study included the preparation of samples, checking the characteristics of simplicia, making extracts by maceration method, and testing the antioxidant activity of ethanol extract of Nipah leaves by using free radical scavenging method using DPPH (1,1-diphenyl-2-pikrilhidrazil), at a wavelength of 515.50 nm, compared with vitamin C as a positive blank, as well as testing the value of the total phenol content of ethanol extract of gallic leaf equivalent of gallic acid (Gallic Acid Equivalent (GAE)) using the colorimetric method using Folin-Ciocalteu reagent at a wavelength of 775 nm and measuring the value of total flavonoid content ethanol extract of Nipah leaves equivalent to quercetin (Quercetin Equivalent (QE)) by colorimetric method using aluminum chloride at a wavelength of 436.50 nm using UV-Spectrophotometry. Antioxidant activity test showed that the Nipah leaf ethanol extract had a very strong antioxidant activity with an IC50 value of 9.662 ppm and a total phenol content of 85.51 mg / g GAE extract and a total flavonoid content of 12, 49 mg / g QE extract.

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Antioxidant activity and total phenolic content in Caulerpa filiformis (Chlorophyta) from Sechura Bay and Paracas Bay, Peru

Revista Peruana de Biología ◽

10.15381/rpb.v27i1.17596 ◽

2020 ◽

Vol 27 (1) ◽

pp. 061-066 ◽

Cited By ~ 1

Author(s):

Joyce Mamani ◽

Jorge Chávez ◽

Eder Apumayta ◽

Patricia Gil-Kodaka

Keyword(s):

Antioxidant Activity ◽

Secondary Metabolites ◽

Methanolic Extract ◽

Total Phenol ◽

North Coast ◽

Total Phenolic ◽

Total Phenol Content ◽

Phytochemical Screening ◽

Phenol Content ◽

The North

In Peru, Caulerpa filiformis is a marine algae listed as an invasive species. For years, its distribution has been considered to be in the north coast (Isla Lobos de Afuera and Piura) until a recent report of its distribution in the central coast (Ancash, Lima, and Ica). The present investigation aims to determine the main groups of secondary metabolites, total phenol content, and antioxidant activity of the methanolic extract of C. filiformis from Sechura Bay (Piura) and Paracas Bay (Ica). The main chemical groups were determined through phytochemical screening, the content of phenols by the Folin–Ciocalteu method, and antioxidant activity by the ABTS method (2,2-azinobis-[3-ethylbenzothiazoline-6-sulfonic acid]) and 2,2′-diphenyl-1-picrylhydrazyl (DPPH). The phytochemical screening of the methanolic extract of C. filiformis from Sechura Bay and Paracas Bay revealed the presence of carbohydrates, polyphenols, tannins, flavonoids, lipids, alkaloids, steroids, and triterpenes for both extracts. The total phenol content of the extract of C. filiformis from Sechura Bay (39.31 ± 0.39 mg of AGE/g extract) was significantly higher (p < 0.05) than that from Paracas Bay (18.78 ± 0.31 mg of AGE/g extract). In the ABTS and DPPH assays, the antioxidant capacity of the Sechura C. filiformis extract (IC50 = 3.49 ± 0.01 and 2.18 ± 0.02 mg/mL) was significantly higher (p < 0.05) than that of the Paracas C. filiformis extract (IC50 = 6.41 ± 0.02 and 2.42 ± 0.04 mg /mL). These findings suggest that the methanolic extract of C. filiformis is a source of secondary metabolites with an antioxidant potential.

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PENGARUH JENIS PELARUT TERHADAP AKTIVITAS ANTIOKSIDAN EKSTRAK MENIRAN (Phyllanthus niruri L.)

Jurnal Ilmu dan Teknologi Pangan (ITEPA) ◽

10.24843/itepa.2020.v09.i04.p10 ◽

2020 ◽

Vol 9 (4) ◽

pp. 458

Author(s):

Rizky Amalia Rachmawati ◽

Ni Wayan Wisaniyasa ◽

I Ketut Suter

Keyword(s):

Antioxidant Activity ◽

Total Phenol ◽

Total Phenol Content ◽

Flavonoid Content ◽

Phyllanthus Niruri ◽

Randomized Design ◽

Completely Randomized Design ◽

Four Levels ◽

Effect On Yield ◽

The Right

This study was conducted to determine the effect of different solvents on the antioxidant activity of gale of the wind extract and to find out the right type of solvent used to extract gale of the wind with the highest antioxidant activity using maceration method. The experimental design used in this study was a completely randomized design (CRD) with a type of solvent as a treatment. The treatment consisting of four levels, namely methanol, ethanol, acetone, isopropanol. All treatments were repeated four times so that they were obtained 16 experimental units. The data obtained were analyzed by variance and if the treatment had significant effect followed by Duncan test. The results showed that the type of solvent treatment had a very significant effect on yield, total phenol, total flavonoids, total tannin, and antioxidant activity (IC50). The results showed that acetone solvent had the best treatment which produced a yield of 22.58%, total phenol content of 188.77 mg GAE/g, total flavonoid content of 247.60 mg QE/g, total tannin content of 297.51mg TAE/g, and antioxidant activity (IC50) of 15.19 ppm.

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Chestnut Drying Is Critical in Determining Aspergillus flavus Growth and Aflatoxin Contamination

Toxins ◽

10.3390/toxins10120530 ◽

2018 ◽

Vol 10 (12) ◽

pp. 530 ◽

Cited By ~ 6

Author(s):

Simona Prencipe ◽

Ilenia Siciliano ◽

Carlotta Gatti ◽

Maria Gullino ◽

Angelo Garibaldi ◽

...

Keyword(s):

Antioxidant Activity ◽

Fungal Growth ◽

Aflatoxin Production ◽

Aflatoxin Contamination ◽

Total Phenol ◽

Fungal Colonization ◽

Total Phenol Content ◽

Phenol Content ◽

Drying Treatment ◽

Drying Conditions

Chestnut drying is used to prevent postharvest losses and microorganism contamination during storage. Several studies reported the contamination by aflatoxins (AFs) produced by Aspergillus spp. in chestnuts. The effect of drying temperatures (from 30 to 50 °C) was evaluated on the growth of A. flavus and the production of aflatoxins in chestnuts. The influence of the treatment on the proximate composition, the total phenol content and antioxidant activity of chestnuts was considered. Fungal colonization was observed on the nuts dried at 30, 35, and 40 °C; the incidence was lower at 40 °C. The highest concentrations of AFB1 and AFB2 were produced at 40 °C. No aflatoxins were detected at 45 or 50 °C. At 40 °C A. flavus was under suboptimal conditions for growth (aw 0.78), but the fungus was able to synthesize aflatoxins. As the temperatures applied increased, the total phenol content increased, while the antioxidant activity decreased. A drying treatment at 45 °C for seven days (aw 0.64) could be a promising method to effectively control both the growth of aflatoxigenic fungi and the production of aflatoxins. This study provides preliminary data useful to improve the current drying conditions used in chestnut mills, to reduce both fungal growth and aflatoxin production.

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Antioxidant activity and total phenol content of blackberries cultivated in a highland tropical climate

Acta Scientiarum Agronomy ◽

10.4025/actasciagron.v39i1.28413 ◽

2017 ◽

Vol 39 (1) ◽

pp. 43 ◽

Cited By ~ 7

Author(s):

Mayara Neves Santos Guedes ◽

Rafael Pio ◽

Luana Aparecida Castilho Maro ◽

Fabíola Fonseca Lage ◽

Celeste Maria Patto de Abreu ◽

...

Keyword(s):

Antioxidant Activity ◽

Free Radical ◽

Phenolic Compounds ◽

High Performance ◽

Radical Scavenging Activity ◽

Radical Scavenging ◽

Total Phenol ◽

Total Phenol Content ◽

Phenol Content ◽

Phenolic Contents

Blackberries are an important option for the diversification of fruit crops. However, there is currently no literature regarding plant cultivation in high-altitude tropical climates. Knowledge of the phenolic composition of blackberries is essential because variations in the levels of these components may exist between cultivars and may depend on environmental conditions. High performance liquid chromatography (HPLC) was used to evaluate the total phenol content of different blackberry cultivars (Arapaho, Brazos, Cainguangue, Cherokee, Choctaw, Comanche, Ébano, Guarani, Tupy and Xavante). Free radical scavenging activity in these cultivars was assayed using a DPPH test. The HPLC-UV chromatogram of blackberry fruit extracts at 280 nm revealed the presence of phenolic compounds. The results showed significant differences in the levels of phenolic compounds in the blackberry cultivars tested. Antioxidant activity was evaluated using the ABTS free radical and ranged from 2.7 ± 0.1 to 19 ± 2 μmole of Trolox equivalents per gram of sample (b.u.). These results are in good correlation with the phenolic contents of the blackberries tested. The Xavante blackberry cultivar had the highest levels of polyphenols that could be individually identified. Catechin polyphenols were found to be the main component in the blackberry varieties tested.

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SCREENING OF TOTAL PHENOL AND FLAVONOID CONTENT IN DIFFERENT CYTOTYPES OF TWO SPECIES OF ACHYRANTHES LINN. FROM WESTERN HIMALAYA, INDIA

International Journal of Pharmacy and Pharmaceutical Sciences ◽

10.22159/ijpps.2017v9i10.19780 ◽

2017 ◽

Vol 9 (10) ◽

pp. 205

Author(s):

Manjit Inder Singh Saggoo ◽

Lovleen .

Keyword(s):

Gallic Acid ◽

Methanol Extract ◽

Intraspecific Variability ◽

Western Himalaya ◽

Total Phenol ◽

Total Phenol Content ◽

Flavonoid Content ◽

Rp Hplc ◽

Superior Genotype ◽

Phenolic Components

Objective: Genus Achyranthes Linn. belonging to family Amaranthaceae consists of six species. The present study was undertaken to screen the phenolic components in the different cytotypes of two species of Genus Achyranthes Linn. growing in western Himalaya, India.Methods: Methanol extract of leaves was used to determine the total phenol and flavonoid contents in different cytotypes of A. aspera Linn. and A. bidentata Blume by spectrophotometric method. Total phenol content was expressed as mg gallic acid g-1phenol and total flavonoid content as mg quercetin g-1flavonoid using the standard curves. Further, gallic acid content in methanol extracts of leaves was determined by RP-HPLC method using C-18 column, employing 0.01% (v/v) orthophosphoric acid: acetonitrile (98:2 v/v) as mobile phase at a flow rate of 1 ml/min with ultraviolet (UV) detection at 272 nm.Results: Hexaploid plants of A. aspera Linn. possess the higher amount of phenol (9.16±0.84 mg/g) and flavonoid (78.36±1.63 mg/g) constituents in the methanol extract of leaves as compared to its dodecaploid counterparts (7.86±0.08 mg/g and 70.20±1.81 mg/g respectively). Similarly, phenol and flavonoid content is found to be more in the methanol extract of leaves of hexaploid plants of A. bidentata Blume (11.93±0.59 mg/g and 115.92±1.32 mg/g respectively) as compared to its dodecaploid counterparts (9.46±0.75 mg/g and 107.76±0.94 mg/g respectively). Further, RP-HPLC analysis of gallic acid revealed that higher amount of gallic acid is present in dodecaploid plants of A. aspera Linn. (1.04±0.02 mg/g) and A. bidentata Blume (1.34±0.03 mg/g) as compared to hexaploid counterparts (1.01±0.01 mg/g and 1.22±0.05 mg/g respectively).Conclusion: The present investigation revealed that A. aspera Linn. and A. bidentata Blume plants show immense intraspecific variability in their phenolic components. Hence there is need to evaluate germplasm to select superior genotype for medicinal and conservation purpose.

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