scholarly journals Comparative Study of Antimicrobial Efficacy of Azithromycin and Cefixime against Salmonella Typhi Infection

2020 ◽  
Vol 6 (1) ◽  
pp. 67-80
Author(s):  
Raj Kumar Thapa ◽  
Sagar Shrestha
Keyword(s):  
Typhoid Fever ◽  
Enteric Fever ◽  
Therapeutic Option ◽  
Systemic Infection ◽  
Salmonella Typhi ◽  
Zone Of Inhibition ◽  
High Concentration ◽  
High Prevalence ◽  
In Vitro Antibacterial Activity

Correction: The page numbers were changed from 84-97 to 67-80 on 31/08/2020. Introduction: Enteric fever is systemic infection caused by the Salmonella enteric serovars typhi and para typhi A B and C. It is the significant cause of morbidity and mortality. It occurs in all parts of the world where water supplied and sanitation is substandard. Annually, it is estimated that more than 10 million cases and 100000 deaths are caused by typhoid fever. Regarding to the strains, a high prevalence of S. typhi and S. paratyphi. A strains in Nepal that showed resistance against the quinolone nalidixic acid (MIC> 256 mcg/ml with a corresponding decreased susceptibility against fluoroquinolones such as ciprofloxacin (MIC>0.125 mcg/ml. Objectives: The main objective of study was to compare the efficacy of Azithromycin and Cefixime in treatment of typhoid fever. Methodology: The in vitro antibacterial activity of azithromycin and Cefixime against 4 isolated colonies of Salmonella typhi from reference of salmonella typhi ATCC no. 14028 and blood culture isolates from three different hospitals was evaluated by disc diffusion (well) method. 0.25 ppm, 0.5 ppm, 4 ppm, 8 ppm, 32 ppm, 128 ppm concentration of both Azithromycin and Cefixime was used. The zone of inhibition was measured and data was analyzed using Excel. Results: In all isolates of Salmonella typhi, the zone of inhibition shown by both Azithromycin and Cefixime is same at low concentration (0.25ppm, 0.5ppm) but with increasing in concentration there is increase in difference in zone of inhibition shown by them. The zone of inhibition shown by Cefixime is greater in high concentration as compared to zone of inhibition shown by Azithromyci. Conclusion: Our result indicate Cefixime is better than Azithromycin in therapeutic option for enteric fever.

scholarly journals In vitro activity of azithromycin in Salmonella isolates from Pakistan

2011 ◽  
Vol 5 (05) ◽  
pp. 391-395 ◽  
Author(s):  
Farhana Butt ◽  
Faisal Sultan
Keyword(s):  
Salmonella Enterica ◽  
Enteric Fever ◽  
Therapeutic Option ◽  
Close Correlation ◽  
Salmonella Typhi ◽  
Public Health Issue ◽  
Disc Diffusion ◽  
Paratyphi B ◽  
High Level

Introduction: Enteric fever is caused by Salmonella enterica serovars Typhi and Paratyphi A, B and C. It is a significant public health issue in Pakistan, which is exacerbated by a high level of resistance some isolates display to drugs routinely used in treatment. Azithromycin may be a treatment option for such isolates. Methodology: We determined the minimum inhibitory concentrations (MICs) of Salmonella Typhi and Paratyphi isolates against azithromycin in an attempt to gauge its feasibility as a therapeutic option. The MICs were also compared with corresponding disc diffusion zone sizes to see if there was consistency between the two tests. We tested 45 Salmonella enterica isolates using E-tests for MIC detection and azithromycin discs with a concentration of 15µg/ml for disc diffusion testing. Results: Salmonella Typhi, Salmonella Paratyphi A, and Salmonella Paratyphi C isolates demonstrated MICs of 2-12mg/L against azithromycin, suggesting that the antibiotic could be used for therapeutic purposes. For Salmonella Paratyphi B, the MICs were 2-48 mg/L. The higher MIC indicates a need for caution when considering use of azithromycin for Salmonella Paratyphi B infections without first testing for the MIC. There was a close correlation between MICs and zone sizes which was statistically significant. Conclusions: Our results indicate azithromycin is a potential therapeutic option for enteric fever. Standardized laboratory testing methods and interpretation for azithromycin  against Salmonella enterica would allow laboratories to report upon this antibiotic with confidence.

scholarly journals Enhanced biofilm and extracellular matrix production by chronic carriage versus acute isolates of Salmonella Typhi

2021 ◽  
Vol 17 (1) ◽  
pp. e1009209
Author(s):  
Aishwarya Devaraj ◽  
Juan F. González ◽  
Bradley Eichar ◽  
Gatan Thilliez ◽  
Robert A. Kingsley ◽  
...  
Keyword(s):  
Typhoid Fever ◽  
Structural Integrity ◽  
Acute Infection ◽  
Systemic Infection ◽  
Salmonella Typhi ◽  
Integration Host Factor ◽  
Extracellular Dna ◽  
Matrix Production ◽  
Chronic Carriage

Salmonella Typhi is the primary causative agent of typhoid fever; an acute systemic infection that leads to chronic carriage in 3–5% of individuals. Chronic carriers are asymptomatic, difficult to treat and serve as reservoirs for typhoid outbreaks. Understanding the factors that contribute to chronic carriage is key to development of novel therapies to effectively resolve typhoid fever. Herein, although we observed no distinct clustering of chronic carriage isolates via phylogenetic analysis, we demonstrated that chronic isolates were phenotypically distinct from acute infection isolates. Chronic carriage isolates formed significantly thicker biofilms with greater biomass that correlated with significantly higher relative levels of extracellular DNA (eDNA) and DNABII proteins than biofilms formed by acute infection isolates. Importantly, extracellular DNABII proteins include integration host factor (IHF) and histone-like protein (HU) that are critical to the structural integrity of bacterial biofilms. In this study, we demonstrated that the biofilm formed by a chronic carriage isolate in vitro, was susceptible to disruption by a specific antibody against DNABII proteins, a successful first step in the development of a therapeutic to resolve chronic carriage.

scholarly journals Template synthesis and characterization of biologically active transition metal complexes comprising 14-membered tetraazamacrocyclic ligand

2010 ◽  
Vol 75 (2) ◽  
pp. 217-228 ◽  
Author(s):  
Dharmpal Singh ◽  
Krishan Kumar ◽  
Ramesh Kumar ◽  
Jitender Singh
Keyword(s):  
Magnetic Measurements ◽  
Macrocyclic Ligand ◽  
Salmonella Typhi ◽  
Molar Conductance ◽  
Biologically Active ◽  
Distorted Octahedral Geometry ◽  
Bacterial Strains ◽  
In Vitro Antibacterial Activity ◽  
Active Transition

A novel series of complexes of the type [M(C28H24N4)X2], where M = Co(II), Ni(II), Cu(II), Zn(II) and Cd(II), X = Cl-, NO3 -, CH3COO- and (C28H24N4) corresponds to the tetradentate macrocyclic ligand, were synthesized by template condensation of 1,8-diaminonaphthalene and diacetyl in the presence of divalent metal salts in methanolic medium. The complexes were characterized by elemental analyses, conductance and magnetic measurements, as well as by UV/Vis, NMR, IR and MS spectroscopy. The low values of the molar conductance indicate non-electrolyte type of complexes. Based on these spectral data, a distorted octahedral geometry may be proposed for all of these complexes. All the synthesized macrocyclic complexes were tested for in vitro antibacterial activity against some pathogenic bacterial strains, viz Bacillus cereus, Salmonella typhi, Escherichia coli and Staphylococcus aureus. The MIC values shown by the complexes against these bacterial strains were compared with the MIC shown by the standard antibiotics linezolid and cefaclor.

scholarly journals Enteric fever with severe pancytopenia in a four year girl

2010 ◽  
Vol 50 (180) ◽  
Author(s):  
R Pathak ◽  
A Sharma ◽  
A Khanal
Keyword(s):  
Bone Marrow ◽  
Enteric Fever ◽  
Hemophagocytic Syndrome ◽  
Systemic Infection ◽  
Salmonella Typhi ◽  
Bone Marrow Suppression ◽  
Major Health Problem ◽  
Major Health ◽  
Severe Pancytopenia ◽  
Paratyphoid Fever

Typhoid fever and paratyphoid fever (also known as enteric fever) are severe systemic illnesses caused by salmonella typhi and S. paratyphi respectively. Enteric fever is prevalent in developing countries including Nepal, where it still remains as a major health problem. There have been reports of pancytopenia with enteric fever which has been attributed to mechanisms like bone marrow suppression, infection associated hemophagocytic syndrome and disseminated intravascular coagulation. We report here a case of severe pancytopenia in enteric fever as a result of bone marrow suppression due to systemic infection. Keywords: enteric fever, pancytopenia, bone marrow suppression

scholarly journals In vitro Antibacterial Activity of Organic Extracts of Aloe barbadensis against Multi-drug resistant Pseudomonas aeruginosa Isolated from Wound Specimens

2018 ◽  
Vol 5 ◽  
pp. 69-76
Author(s):  
Mamata Adhikari ◽  
Anil Kumar Sah ◽  
Dev Raj Joshi
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Antibacterial Activity ◽  
Inhibitory Action ◽  
Therapeutic Option ◽  
Diffusion Method ◽  
Drug Resistant ◽  
Aloe Barbadensis ◽  
Organic Extracts ◽  
In Vitro Antibacterial Activity

Objectives: In order to investigate alternate therapeutic option, this study was carried out to assess the in vitro antibacterial activity of gel extract of Aloe barbadensis against multiple antibiotic resistant Pseudomonas aeruginosa isolated from wound specimens. Methods: A total of 180 different wound specimens collected in a hospital, were subjected to isolate and identify P. aeruginosa by cultural methods. Antibiotic susceptibility testing was done by Kirby- Bauer disc diffusion method to screen multidrug resistant isolates. A. barbadensis extracts were prepared using aqueous and organic solvents and their in vitro inhibitory action was evaluated by agar well diffusion methods. Results: Out of total, 38 (21.1%) of the wound specimens showed the occurrence of P. aeruginosa, among which 15 (39%) isolates were multi-drug resistant. Organic extracts of various concentrations (0.2 - 0.8 v/v %) inhibited 66.7% of MDR and all non-MDR (n = 23) P. aeruginosa with zone of inhibition ranging from 9.5 ±1.0 to 21.3 ± 2.2 mm but not by aqueous extract. A positive Pearson’s correlation (r=0.983) was found between antibacterial effect and concentrations of the extracts. The antibacterial activity of organic extracts was statistically associated with antibiotic resistance profile of the organism (p<0.05). Conclusion: Organic extracts of A. barbadensis revealed variable in vitro inhibitory action against both MDR and non-MDR P. aeruginosa isolated from wound specimens. Although further confirmation is needed, aloe gel extract may be applied as an alternate treatment option.

scholarly journals Drug-Resistant Salmonella typhi in Pakistan

2019 ◽  
Vol 59 (1) ◽  
pp. 31-33 ◽  
Author(s):  
Christine E. Petrin ◽  
Russell W. Steele ◽  
Elizabeth A. Margolis ◽  
Justin M. Rabon ◽  
Holly Martin ◽  
...  
Keyword(s):  
United States ◽  
Typhoid Fever ◽  
Enteric Fever ◽  
The United States ◽  
Salmonella Typhi ◽  
Antimicrobial Treatment ◽  
Third Generation ◽  
Drug Resistant ◽  
Oral Transmission ◽  
Third Generation Cephalosporins

Enteric fever (formerly typhoid fever) is a bacterial illness caused by fecal-oral transmission of Salmonella typhi or paratyphi. In early 2018, an outbreak of Salmonella typhi resistant to third-generation cephalosporins, ampicillin, ciprofloxacin, trimethroprim-sulfamethoxazole, and chloramphenicol was reported in Pakistan. This strain, termed “extensively resistant typhi,” has infected more than 5000 patients in endemic areas of South Asia, as well as travelers to and from these areas, including 5 cases in the United States. We present the case of one such child who developed extensively resistant enteric fever during a recent visit to Pakistan and required broader antimicrobial treatment than typically required. Clinicians should be aware that incoming cases of enteric fever may be nonsusceptible to commonly recommended antibiotics and that extensively resistant typhi requires treatment with carbapenems such as meropenem or azithromycin.

scholarly journals Antimicrobial evaluation of methyl 4-O-Acetyl-α-L-Rhamnopyranoside derivatives

2013 ◽  
pp. 33-43
Author(s):  
Mohammed M Matin ◽  
Mohammad Ibrahim ◽  
Md Shafiqur Rahman
Keyword(s):  
Pathogenic Bacteria ◽  
Pathogenic Fungi ◽  
Macrophomina Phaseolina ◽  
Antibacterial Activities ◽  
Salmonella Typhi ◽  
Shigella Dysenteriae ◽  
Curvularia Lunata ◽  
In Vitro Antibacterial Activity ◽  
Antimicrobial Evaluation

A number of 2,3-di-O-acyl derivatives (6-11) of methyl 4-O-acetyl-a-Lrhamnopyranoside (5) obtained by using various acylating agents were screened for in vitro antifungal activity against four plant pathogenic fungi, viz., Alternaria alternata, Curvularia lunata. Fusarium equiseti and Macrophomina phaseolina. These compounds were also screened for in vitro antibacterial activity against ten human pathogenic bacteria, viz., Bacillus subtilis, Bacillus cereus, Bacillus megaterium, Staphylococcus aureus, Escherichia coli, INABA ET (Vibrio), Pseudomonas species, Salmonella paratyphi, Shigella dysenteriae and Salmonella typhi. The study reveal that these 4-O-acetyl-?-L-rhamnopyranoside derivatives are more prone towards antifungal activities than that of antibacterial activities. DOI: http://dx.doi.org/10.3329/cujbs.v3i1.13404 The Chittagong Univ. J. B. Sci.,Vol. 3(1&2):33-43, 2008

scholarly journals Chemical Composition and in Vitro Antibacterial Activity of the Essential Oil of Minthostachys Mollis (Kunth) Griseb Vaught from the Venezuelan Andes

2009 ◽  
Vol 4 (7) ◽  
pp. 1934578X0900400 ◽  
Author(s):  
Flor D. Mora ◽  
María Araque ◽  
Luis B. Rojas ◽  
Rosslyn Ramírez ◽  
Bladimiro Silva ◽  
...  
Keyword(s):  
Antibacterial Activity ◽  
Essential Oil ◽  
Chemical Constituents ◽  
Salmonella Typhi ◽  
Inhibitory Effect ◽  
Gram Negative Bacteria ◽  
Gram Negative ◽  
Significant Inhibitory Effect ◽  
In Vitro Antibacterial Activity

Chemical constituents of the essential oil from the leaves of Minthostachys mollis (Kunth) Griseb Vaught var. mollis collected in January 2008 at Tuñame, Trujillo State, Venezuela, were separated and identified by GCMS analysis. The essential oil was obtained by hydrodistillation and thirteen components (98.5% of the sample) were identified by comparison with the Wiley GCMS library data base. The two major components were pulegone (55.2%) and trans-menthone (31.5%). The essential oil showed a significant inhibitory effect against Gram-positive and Gram-negative bacteria, especially Bacillus subtilis and Salmonella typhi (4 μg/mL).

scholarly journals Fitness benefits in fluoroquinolone-resistant Salmonella Typhi in the absence of antimicrobial pressure

eLife ◽  
2013 ◽  
Vol 2 ◽  
Author(s):  
Stephen Baker ◽  
Pham Thanh Duy ◽  
Tran Vu Thieu Nga ◽  
Tran Thi Ngoc Dung ◽  
Voong Vinh Phat ◽  
...  
Keyword(s):  
Target Genes ◽  
Systemic Infection ◽  
Critical Factor ◽  
Selective Advantage ◽  
Salmonella Typhi ◽  
Antimicrobial Treatment ◽  
Resistance Mutations ◽  
Resistant Strains ◽  
And Control

Fluoroquinolones (FQ) are the recommended antimicrobial treatment for typhoid, a severe systemic infection caused by the bacterium Salmonella enterica serovar Typhi. FQ-resistance mutations in S. Typhi have become common, hindering treatment and control efforts. Using in vitro competition experiments, we assayed the fitness of eleven isogenic S. Typhi strains with resistance mutations in the FQ target genes, gyrA and parC. In the absence of antimicrobial pressure, 6 out of 11 mutants carried a selective advantage over the antimicrobial-sensitive parent strain, indicating that FQ resistance in S. Typhi is not typically associated with fitness costs. Double-mutants exhibited higher than expected fitness as a result of synergistic epistasis, signifying that epistasis may be a critical factor in the evolution and molecular epidemiology of S. Typhi. Our findings have important implications for the management of drug-resistant S. Typhi, suggesting that FQ-resistant strains would be naturally maintained even if fluoroquinolone use were reduced.

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