scholarly journals Phytochemical Screening of Medicinal Plants and Study of the Effect of Phytoconstituents in Seed Germination

2020 ◽  
Vol 35 (2) ◽  
pp. 1-11
Author(s):  
Tinky Sharma ◽  
Binjita Pandey ◽  
Bishnu Kumar Shrestha ◽  
Gayatri Maiya Koju ◽  
Rojeena Thusa ◽  
...  
Keyword(s):  
Cell Proliferation ◽  
Seed Germination ◽  
Medicinal Plants ◽  
Pisum Sativum ◽  
Cytotoxic Effect ◽  
Methanol Extract ◽  
Living Cells ◽  
Phytochemical Screening ◽  
Volatile Oils ◽  
Plant Parts

 Phytochemical screening of ten different locally available plant parts was done in methanol extract. Tannin, quinine, terpenoid, flavonoid, steroid, alkaloid, cardiac glycoside, glycoside, volatile oils, etc were the phytoconstituents found in plants. The study of the effect of phytoconstituents in the germination of Pisum sativum seeds revealed that the phytoconstituents present in plant extracts showed the cytotoxic effect in living cells i.e. in germinating Pisum sativum seeds. The phytoconstituents present in the plant extract showed an effect on cell proliferation and growth. Hence these plants could be used to develop drugs against cancer cells and also may be effective against microbes and bacteria

scholarly journals SCREENING OF PHYTOCHEMICALS AND QUANTITATIVE ESTIMATION OF TOTAL FLAVONOIDS AND PHENOLIC COMPOUNDS OF LAGASCEA MOLLIS CAV. (ASTERACEAE)

2014 ◽  
Vol 1 (2) ◽  
pp. 94-97
Author(s):  
Saradha M ◽  
Paulsamy S ◽  
Abinaya G
Keyword(s):  
Phenolic Compounds ◽  
Methanol Extract ◽  
Quantitative Estimation ◽  
Root Extract ◽  
Total Flavonoids ◽  
Phytochemical Screening ◽  
Plant Parts ◽  
Soxhlet Apparatus ◽  
Phytochemical Constituents ◽  
Leaves And Roots

Aim of the study was to analyze the phytochemical constituents and estimation of total flavonoids and phenolic compounds of leaves and roots of the medicinal plant Lagascea mollis. Methanol extract of dried leaves and roots of L. mollis was prepared by using soxhlet apparatus. The extract prepared was tested forpreliminary qualitative phytochemical screening, followed by the quantitative estimation of total flavonoids and phenols by spectroscopy. The selected plant parts were found to contain alkaloids, flavonoids and terpenoids in both parts and saponin is present only in leaf. In quantitative estimation the reports revealedthe presence 0.017 and 0.013 mgRE/g extract and 458.91 and 704.50 mgGAE/g extract total flavonoids and phenolic compounds in leaf and root extract respectively. These major phytoconstituents present in this species may be accounted as factors for the medicinal importance of L. mollis.

scholarly journals Cytological changes induced by clethodim in Pisum sativum plant

2020 ◽  
Vol 49 (2) ◽  
pp. 367-374
Author(s):  
Sazada Siddiqui ◽  
Sulaiman Alrumman
Keyword(s):  
Seed Germination ◽  
Pisum Sativum ◽  
Mitotic Index ◽  
Cytotoxic Effect ◽  
Chromosomal Anomalies ◽  
Dependent Manner ◽  
Cytological Changes ◽  
Dose Dependent ◽  
Radicle Length ◽  
Mitotic Cells

Effects of clethodim treatment on mitotic cells of P. sativum were studied in the present investigation. Pisum sativum seeds are treated with different concentrations of clethodim varying from 0.01, 0.02, 0.03 and 0.04% for 1 and 2 hrs and their effect on seed germination, radicle length, mitotic index and chromosomal anomalies were investigated. The outcomes reveal that treatment of clethodim on P. sativum seeds diminishes seed germination, radicle length, and mitotic index in dose-dependent manner. Also, in clethodim treated seeds which are time and concentration dependent, an escalation in the proportion of abnormal mitotic phases was observed. C-mitosis, fragments, precocious separation, stickiness and bridges were the most frequently observed deviations. The results revealed that frequently used herbicide clethodim has a substantial cytotoxic effect on P. sativum.

scholarly journals Phytochemical screening, antibacterial and antifungal activity of Pinda concanensis (Dalzell) P.K. Mukh. & Constance (Apiaceae) an endemic plant from Nasik district (M.S.) India

2017 ◽  
Vol 6 (12) ◽  
pp. 1886 ◽  
Author(s):  
Hemantkumar Atmaram Thakur
Keyword(s):  
Antimicrobial Activity ◽  
Medicinal Plants ◽  
Economic Value ◽  
Water Extract ◽  
Diffusion Method ◽  
Phytochemical Analysis ◽  
Phytochemical Screening ◽  
Endemic Plant ◽  
Plant Parts ◽  
Agar Disc

Medicinal plants are a source of great economic value all over the world. Various medicinal plants have been used for years in daily life to treat diseases. Present study illustrates the phytochemical screening and antimicrobial activity of Pinda concanensis (Dalzell) P.K. Mukh. & Constance. The selected plant Fruits were collected and powdered. The bioactive compounds were extracted by using Methanol, Ethanol, Petroleum ether and water in a soxhlet extractor. The antimicrobial activity was determined by using agar disc and well diffusion method. Acetone and chloroform extracts were mixed with 1ml diethyl sulfoxide (DMSO) and added into the well. The extract of Methanol with Pinda concanensis (Dalzell) P.K. Mukh. & Constance has shown maximum zone of inhibition against bacterial pathogens when compared to chloral and water extract. Phytochemical analysis reflected the antimicrobial activity of Pinda concanensis (Dalzell) P.K. Mukh. & Constance which is due to the presence of phytochemical compounds like alkaloids, terpenoids, steroids, flavonoids, Saponins, Phenolic compounds and tannins. The results of agar well diffusion method indicated the inhibition and it depends largely upon plant parts used and organism tested.

scholarly journals Phytochemical screening, antimicrobial activity and cytotoxicity of Nepalese medicinal plants Swertia chirayita and Dendrobium amoenum

2015 ◽  
Vol 3 (1) ◽  
pp. 48-57 ◽  
Author(s):  
Pritish Shrestha ◽  
Manisha Bista ◽  
Prativa Sharma ◽  
Shristi Shrestha ◽  
Basanta Lamichhane ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Medicinal Plants ◽  
Ethyl Acetate ◽  
Brine Shrimp ◽  
Methanol Extract ◽  
Ethyl Acetate Extract ◽  
Phytochemical Screening ◽  
Zone Of Inhibition ◽  
Swertia Chirayita ◽  
Percentage Yield

Research on medicinal plants are important to Nepal because most of its rural population relies on it as mode of medicine. Medicinal plants namely Swertia chirayita and Dendrobium amoenum were collected from mid hills of Nepal. The present study was undertaken to find the antimicrobial activity, phytochemical presence and their cytotoxicity in different extraction medium. The percentage yield from the plants were highest in warm methanol extraction with 12.6%, followed by ethyl acetate and lowest was for cold methanol. Plant extract showed the presence of antioxidants like alkaloid, terpenoids, flavonoids, tannin, glycosides. The Brine Shrimp Bioassay of methanol and ethyl acetate extract showed cytotoxicity. Chiraito extract showed LC50 of 199 ppm for Dhunche sample, 128.82 ppm for Daman sample and 131.82 ppm of Illam sample. The antibacterial activity of methanol extract of Chiraito and Dendrobium amoenum showed significant bioactivity by inhibiting growth of microbial species selected for the test. The zone of inhibition shown by the extracts was comparable to the standard antibiotics. Similarly, methanol extract of Chiraito also showed significant antifungal activity with the zone of inhibition comparable to amphotericin.Nepal Journal of Biotechnology. Dec. 2015 Vol. 3, No. 1: 48-54

A COMPARATIVE STUDY OF IN VITRO ANTIMICROBIAL ACTIVITY AND TLC STUDIES OF PETALS OF SELECTED INDIAN MEDICINAL PLANTS

2016 ◽  
Vol 9 (5) ◽  
pp. 259 ◽  
Author(s):  
Sumathy Rengarajan ◽  
Vijayalakshmi Melanathuru ◽  
Deecaraman Munuswamy ◽  
Sankaranarayanan Sundaram ◽  
Saravanan Thiruverkadu Selvaraj
Keyword(s):  
Antimicrobial Activity ◽  
Antibacterial Activity ◽  
Medicinal Plants ◽  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Methanol Extract ◽  
Phytochemical Screening ◽  
Layer Chromatography ◽  
Indian Medicinal Plants

ABSTRACTObjective: The present study was to evaluate the in vitro antibacterial activity, and thin-layer chromatography (TLC) studies from the petals of fourdifferent Indian medicinal plants (Punica granatum, Hibiscus rosa-sinensis, Cassia auriculata, and Moringa oleifera).Methods: The phytochemical screening of the methanol extract of petals of four different Indian medicinal plants was performed using standardprocedures. The antimicrobial activity was tested against various test organisms using the agar disc diffusion method.Results: The preliminary phytochemical screening for petals of four different medicinal plants revealed the presence of flavonoids, alkaloids, tannins,and saponins. From the above study, the results indicated that the methanol extract of M. oleifera petals showed the highest antimicrobial activityagainst Staphylococcus aureus and Bacillus subtilis with zone of inhibition 17.93 and 23.40, respectively, at the concentration of 20 µl/ml and alsoshowed the maximum inhibitory activity at the highest concentration (20 µl/ml) than the lowest concentration (5 µl/ml) against Gram-negativebacteria such as Escherichia coli, Proteus vulgaris, Pseudomonas aeruginosa, and Gram-positive B. subtilis and S. aureus. TLC studies of methanolextracts of petals of Indian medicinal plants revealed the presence of different phytoconstituents as evidenced by separated compounds with differentRf values.Conclusion: The results obtained in the present study indicate that the petals of four different Indian medicinal plants showed the highest antibacterialactivity and can be used as an antibacterial agent against bacterial diseases.Keywords: Phytochemicals, Antibacterial activity, Thin-layer chromatography.

Effects of Papaya Leaf Extract (Carica papaya L.) on Cellular Proliferation and Apoptosis in Cervical Cancer Mice Model

2019 ◽  
Vol 17 (5) ◽  
pp. 265-275
Author(s):  
Y. Peristiowati ◽  
Y. Puspitasari ◽  
Indasah
Keyword(s):  
Cervical Cancer ◽  
Cell Proliferation ◽  
Hela Cells ◽  
Leaf Extract ◽  
Caspase 3 ◽  
Methanol Extract ◽  
Negative Control ◽  
Proliferation Index ◽  
Control Group ◽  
P53 Expression

This study is aimed at analyzing the anticancer properties of papaya leaf extract, specifically the inhibition of cell proliferation and apoptotic induction through nuclear factor kappa-light-chain-enhancer of activated B cells (NF-κB) and p53 pathways. Twenty-five mice (Mus musculus), aged 2 months and weighing 20–30 g, was injected with 0.5 mg dexamethasone for 7 days. The mice were then injected intracutaneously with 1 ml of HeLa cells (8 × 106 HeLa cells/microliter). The mice were divided into five groups (5 each): negative control (P1) (5% CMC-Na, sodium carboxymethyl cellulose), treatment II (225 mg/kg BW (body weight) papaya leaves methanol extract), treatment III (450 mg/kg BW), treatment IV (750 mg/kg BW), and treatment PV (2 mg alcohol anticancer drug). Papaya leaf extract treatments were applied for 2 weeks. Then, the tumor tissue was isolated for hematoxylin and eosin staining. Immunohistochemical imaging was used to detect Ki-67, caspase-3, NF-κB, and p53 expression. Further analysis was undertaken using the ImmunoRatio software program. The results indicated that administration of papaya leaf methanol extract significantly increased the expression of NF-κB and p53 at a dose of 450 mg/kg BW. Our results also showed that the mice treated with 450 mg of papaya leaf extract per kg of BW (P3) had the largest increase of caspase-3 expression compared to the negative control group. Papaya leaf ethanol extract decreased the cancer cell proliferation index and increased apoptosis of cancer cells in animal models of cervical cancer; it may also work to increase NF-kB expression and expression of the p53 gene.

Phytochemical Screening, Antimicrobial and Antioxidant Activities of Selected Mangrove Species

2020 ◽  
Vol 16 (2) ◽  
pp. 152-163 ◽  
Author(s):  
Nilesh L. Dahibhate ◽  
Utpal Roy ◽  
Kundan Kumar
Keyword(s):  
Staphylococcus Epidermidis ◽  
Methanol Extract ◽  
Ethyl Acetate Extract ◽  
Antioxidant Activities ◽  
Avicennia Marina ◽  
Total Flavonoids ◽  
Phytochemical Screening ◽  
Mangrove Species ◽  
Sonneratia Caseolaris ◽  
Bruguiera Gymnorhiza

Background: Mangrove plant extracts are used in folkloric medicine as aphrodisiac, sedative, antioxidant, antimicrobial and antimalarial. Screening for antimicrobial, phytochemical and antioxidant activities of fourteen mangroves plant species (Aegiceras corniculatum, Acanthus ilicifolius, Avicennia alba, Avicennia marina, Avicennia officinalis, Bruguiera cylindrica, Bruguiera gymnorhiza, Ceriops tagel, Excoecaria agallocha, Kandelia candel, Rhizophora apiculata, Rhizophora mucronata, Sonneratia alba, and Sonneratia caseolaris) in various solvents are reported in this work. Methods: The antimicrobial screening was carried out using agar well diffusion method. In this study, nine pathogenic strains were used, including three Gram-positive and six Gram-negative bacteria. Phytochemical screening, total flavonoids, total phenolic and antioxidant activity was tested by DPPH radical scavenging assay. Four phenolic compounds (gallic acid, vanillin, tannic acid and quercetin) were quantified by LC-MS/MS in selected mangrove species. Results: Antimicrobial screening showed Staphylococcus aureus, Staphylococcus epidermidis, Enterococcus faecalis and Pseudomonas aeruginosa were most susceptible to ethyl acetate extract as compared to ethanol and methanol extract. Ethyl acetate extract of Avicennia marina and Bruguiera gymnorhiza showed strong antibacterial activity against Staphylococcus aureus, Staphylococcus epidermidis, Enterococcus faecalis and Pseudomonas aeruginosa. Phytochemical analysis revealed the presence of saponins, phenolics, flavonoids, alkaloids, tannins, and terpenoids, which was found to be variable as per the solvent used for extraction. In addition, total phenolics and total flavonoids content with different solvents were found in the range of 11.08 to 196.76 mg GAE/g and 12.92 to 110.3 mg QE/g of extract respectively. Moreover, antioxidant capacities expressed in terms of IC50 (mg/mL) showed that methanol extract exhibited higher antioxidant capacity followed by ethanol extract. LC-MS/MS analysis showed gallic acid and tannic acid are present in higher concentration in Aegiceras corniculatum and Sonneratia caseolaris than other species. The vanillin and quercetin were found in the least concentration. Conclusion: Mangroves species are rich source of antioxidant, phenolics and antimicrobial compounds.

Medicinal plants and sustainable livelihood in Pauri district of Garhwal Himalaya, Uttarakhand, India.

2016 ◽  
Vol 5 (06) ◽  
pp. 4589 ◽  
Author(s):  
Vardan Singh Rawat
Keyword(s):  
Environmental Management ◽  
Medicinal Plants ◽  
Plant Species ◽  
Skin Diseases ◽  
Sustainable Livelihoods ◽  
Vital Role ◽  
Local Communities ◽  
Sustainable Livelihood ◽  
Plant Parts ◽  
Total Plant

The present study was conducted in the Thalisain block of Pauri Garhwal to document the medicinal plants used by the local communities. 53 plant species distributed in 38 families were documented. Of the total plant species 49% were herbs, 26% trees, 23% shrubs and 2% climbers. 16 different plant parts were used by local communities for different ailments. Medicinal plants were widely used by major sections of the community against common colds, cough, skin diseases, snake bite, fever, joint pains, bronchitis etc. Women and local healers called vaids have a vital role in environmental management due to traditional knowledge and use of plants as medicine with undocumented knowledge. It has been observed as one of the best option of sustainable livelihoods for the residents of the area.

scholarly journals Assessment of Antimalarial Activity againstPlasmodium falciparumand Phytochemical Screening of Some Yemeni Medicinal Plants

2009 ◽  
Vol 6 (4) ◽  
pp. 453-456 ◽  
Author(s):  
Mohammed A. Alshawsh ◽  
Ramzi A. Mothana ◽  
Hassan A. Al-shamahy ◽  
Salah F. Alsllami ◽  
Ulrike Lindequist
Keyword(s):  
Medicinal Plants ◽  
Antimalarial Activity ◽  
Traditional Healers ◽  
Antiplasmodial Activity ◽  
World Health ◽  
Moderate Activity ◽  
Phytochemical Screening ◽  
Cissus Rotundifolia ◽  
Health Organization

Developing countries, where malaria is one of the most prevalent diseases, still rely on traditional medicine as a source for the treatment of this disease. In the present study, six selected plants (Acalypha fruticosa,Azadirachta indica,Cissus rotundifolia,Echium rauwalfii,Dendrosicyos socotranaandBoswellia elongata) commonly used in Yemen by traditional healers for the treatment of malaria as well as other diseases, were collected from different localities of Yemen, dried and extracted with methanol and water successfully. The antiplasmodial activity of the extracts was evaluated against fresh clinical isolates ofPlasmodium falciparum. The selectivity parameters to evaluate the efficacy of these medicinal plants were measured byin vitromicro test (Mark III) according to World Health Organization (WHO) 1996 & WHO 2001 protocols of antimalarial drug tests. Among the investigated 12 extracts, three were found to have significant antiplasmodial activity with IC50values less than 4 µg/ml, namely the water extracts ofA. fruticosa,A. indicaandD. socotrana. Six extracts showed moderate activity with IC50values ranging from 10 to 30 µg/ml and three appeared to be inactive with IC50values more than 30 µg/ml. In addition, preliminary phytochemical screening of the methanolic and aqueous extracts indicated the presence of saponins, tannins, flavonoids, terpenoids, polysaccharides and peptides.

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