scholarly journals Evaluation of the Possible Immuno-Protective Effect of Nigella Sativa Seed Oil on Cyclophosphamide- Induced Myelosuppression in Mice

2020 ◽  
Vol 29 (1) ◽  
pp. 253-259
Author(s):  
Shahad A. Alsaad ◽  
Sarmed H. Kathem
Keyword(s):  
Seed Oil ◽  
Nigella Sativa ◽  
Negative Control Group ◽  
Negative Control ◽  
Control Group ◽  
Limiting Factor ◽  
Mice Model ◽  
Hematopoietic Stem ◽  
Life Threatening ◽  
Nigella Sativa Seed

Myelosuppression is one of the serious adverse effects of cancer chemotherapy that lead to life threatening febrile neutropenia and considered a limiting factor for successful therapy. Cyclophosphamide a widely used anticancer drugs, induces severe bone marrow suppression by damaging hematopoietic stem cells. As cancer incidence expands globally, the demand for an effective myeloprotective therapy during cancer treatment is also increasing.Nigella sativa seed oil, a well-known plant extract that widely used for various health conditions. This study aims to evaluate the myeloprotective activity of Nigella sativa seed oil in cyclophosphamide-induced myelosuppression mice model. Myelosuppression induced by single intraperitoneal injection of cyclophosphamide (200 mg/kg). Animals were divided into 4 groups each with 6 mice. First group served as negative control group received only normal saline. A second group served as experimental myelosuppression model group achieved by 

Effect of HylocereusPolyrhizus Rind Extract Toward Interleukin-1β, Vascular Endothelial Growth Factor Expression, Endometriosis Implant Area

2017 ◽  
Vol 9 (08) ◽  
Author(s):  
YanuarEka P. ◽  
Hendy Hendarto ◽  
Widjiati .
Keyword(s):  
Vascular Endothelial Growth Factor ◽  
Growth Factor ◽  
Treatment Group ◽  
Negative Control Group ◽  
Negative Control ◽  
Control Group ◽  
Vascular Endothelial ◽  
Mice Model ◽  
Endothelial Growth Factor ◽  
Fruit Rind

Retrograde menstruation lead to I Kappa B Kinase (IKK) fosforilation in peritoneum macrophage and cause secretion of proinflammatory cytokine interleukin1β then stimulate endometriosis cell to produce Vascular Endothelial Growth Factor which lead to increasing of endometriosis lession seen as endometriosis implant area. Cytokine secretion was inhibited through prevention of NF-κB activation by dragon red fruit rind extract (Hylocereuspolyrhizus). The aim of this reserach is to know the effect of dragon red fuit rind extract with 0,25; 0,5; and 1 mg/g bodyweight dosage toward IL-1β, VEGF expression and implant area in endometriosis mice model. The design of this experiment was randomized post test only control group design.Endometrios mice model were made in 14 days and split into two group, positive control group and treatment group after two week negative control group and postive control group were given Na-CMC 0,5% solution consequetively, and treatment group were given dragon red fruit extract with different dosage. Signification number for IL-1β is p>0,05, signification number for VEGF is p>0,05, and implant area signification number is p>0,05. Administration of dragon red fruit rind extract can decrease IL-1β, VEGF, and implant area.

scholarly journals Antiproliferation Effects of Nanophytosome-Loaded Phenolic Compounds From Fruit of Juniperus Polycarpos Against Breast Cancer in Mice Model: Synthesis, Characterization and Therapeutic Effects

2021 ◽  
Author(s):  
Soheila Moeini ◽  
Ehsan Karimi ◽  
Ehsan Oskoueian
Keyword(s):  
Breast Cancer ◽  
Negative Control Group ◽  
Negative Control ◽  
Control Group ◽  
Therapeutic Effects ◽  
Mice Model ◽  
Fruit Extract ◽  
Phenolic Fraction ◽  
Juniperus Polycarpos

Abstract Background: This research was performed to synthesize nanophytosomes-loaded high phenolic fraction (HPF) from Juniperus polycarpos fruit extract and investigate its antiproliferation effects against breast cancer in mice model. Results: The nanophytosomes-loaded HPF from Juniperus polycarpos fruit extract was synthesized. The mice trial was conducted to determine the possible toxic effects of the synthesized nanophytosomes. The anticancer, pro-apoptotic, and antioxidative activities of the nanophytosomes were determined. The nanophytosomes-loaded HPF had a spherical structure with a size of 176 nm and a polydispersity index coefficient of 0.24. The in-vivo study manifested that nanophytosomes-loaded HPF significantly improved weight gain and food intake compared to the negative control group (p<0.05). The nanophytosomes-loaded HPF significantly enhanced the expression of bax (3.4-fold) and caspase-3 (2.7-fold) genes but reduced bcl2 (3.6-fold) gene expression in tumor cells. The average tumor size was significantly decreased in mice treated with nanophytosomes-loaded HPF (p<0.05). The expression of GPX (2.3-fold) and SOD (2.7-fold) antioxidants in the liver of mice supplemented with nanophytosomes-loaded HPF was significantly developed compared to the negative control (p<0.05). The nanophytosomes-loaded HPF did not show toxicity on normal cells. Conclusion: Our results indicated that nanophytosomes-loaded HPF might be a potential anticancer agent for the breast cancer treatment.

scholarly journals Dermal application of lactic acid based cream of a non pathogenic Kocuria marina (BMKO1) strain against Epidermophyton floccosum (MTCC 613) symptomatic excision mice model

2019 ◽  
Vol 9 (1-s) ◽  
pp. 113-120
Author(s):  
Soumya S Dash ◽  
Smaranika Pattnaik
Keyword(s):  
Lactic Acid ◽  
Mouse Models ◽  
Drug Application ◽  
Clinical Signs ◽  
Negative Control Group ◽  
Negative Control ◽  
Control Group ◽  
Weight Changes ◽  
Mice Model ◽  
Epidermophyton Floccosum

The aim of this study was to evaluate the antifungal efficacy of Kocuria marina (BMKo1) derived Lactic acid against Epidermophyton floccosum (MTCC-613) infections induced on male Swiss Albino mice model (Mus musculus).  For this purpose, the isolated strain was subjected to ‘flask fermentation’ and the Lactic acid produced as fermentation product, was quantified and analysed. Prior to preclinical test, healthy mice models of approximately 8 weeks old and 25-30 gm (weight) were subjected to intra-dermal administration for a period of 15 days to test for toxicity. Mortality, clinical signs, body weight changes were continually monitored. Then the mouse models were inoculated with 100 µl/ml (V/V) of E. floccosum (MTCC-613) spore suspensions following ‘Excision model’. After induction of the infection, the symptomatic mice groups were subjected to topical application of Kocuria lactic acid cream based formulation at a concentration of 1µl/ml (V/V). The naked eye observations were made on the infected lesions till the absolute deduction of infection of excised skin surfaces. The degrees of deduction of infection were converted into scores and the percentages (%) of deduction of infection were calculated and the average value was derived. There were inclusion of positive control (Fluconazole) and negative control (group with infection induced excision, but without any drug application) mice groups for the sake of comparison. Further, with absolute deduction of infection score observed in mice group, applied with Kocuria derived Lactic acid was akin to Fluconazole activity. However, the infection induced mice group was found to be with substantial increase of degree of infection. This study have curtain raised about the anti Epidermophyton infection activity of a cream based  Cell free Lactic acid derived from a non pathogenic strain of Kocuria marina on mouse models. Keywords: Kocuria marina, Epidermophyton floccosum, Lactic acid

scholarly journals Ethanol Extract of Black Cumin Seed (Nigella Sativa) Reduces Expression Kidney iNOS in Mice Model Preeclampsia

2019 ◽  
Vol 5 (2) ◽  
pp. 88
Author(s):  
Rubiati Hipni
Keyword(s):  
Nigella Sativa ◽  
Ethanol Extract ◽  
Negative Control ◽  
Inos Expression ◽  
Control Group ◽  
Mice Model ◽  
Control Group Design ◽  
Black Cumin ◽  
Black Cumin Seed ◽  
Cumin Seed

This study aims to analyze the effect of ethanol extract of black cumin seeds (Nigella Sativa) on iNOS expression in the kidney of mice model preeclampsia. Design This study uses an experimental design with a posttest only control group design. Using a mice model preeclampsia. This study was divided into 6 groups: negative control, positive control (preeclampsia model), model + black cumin seed extract 500 mg/KgBB/day, 1000 mg/KgBB/day, 1500 mg/KgBB/day, and 2000 mg/KgBB/day days, after surgery on mice then examined iNOS Kidney expression by methods Immunohistochemical. Data from observations were analyzed by one way ANOVA test. The result there is an effect of ethanol extract of black cumin seeds on renal iNOS expression in mice model preeclampsia (p <0.05) at doses of 500mg, 1000mg, 1500mg, and 2000mg on decreasing renal iNOS expression. The conclusion Ethanol extract of black cumin seeds (Nigella sativa) can reduce iNOS Kidney expression in Mice Preeclampsia.

scholarly journals Protective Effect of Combination Commercial Black Seed Oil (Nigella sativa) and Honey Against Cisplatin-Induced Hepatotoxicity in Rats

2020 ◽  
Vol 1 (2) ◽  
pp. 43
Author(s):  
Andri Muhrim Siddiq ◽  
Muhammad In'am Ilmiawan ◽  
Mitra Handini
Keyword(s):  
Oxidative Stress ◽  
Protective Effect ◽  
Rat Liver ◽  
Liver Tissue ◽  
Seed Oil ◽  
Negative Control Group ◽  
Negative Control ◽  
Control Group ◽  
Black Seed ◽  
Black Seed Oil

Background: The chemotherapeutic use of cisplatin (CP) is restricted because of its hepatotoxicity induced by oxidative stress. Malondialdehyde (MDA) is a secondary product of lipid peroxidation as a biomarker of oxidative stress. Individual administration of black seed oil (BSO) or honey (H) demonstrated hepatoprotective effect in rats. Interaction of both substances when administrated as combination can be evaluated using combination index (CI) to quantitatively depict synergism (CI<1), additive (CI=1) and antagonism effect (CI>1). Objective: to know the combination effect of BSO and honey on rat liver tissue given CP exposure. Methods: This study used 30 rats were divided into 10 groups. Normal group (N); Negative control group (NC); P1-P4 groups were administerated BSO (1 and 2 mL/kg) and honey (3.7 and 7.4 mL/kg); P5-P8 groups were combination of BSO and H. P1-P8 groups were given BSO and honey orally for 21 days. On the 18th day, NC and P1-P8 groups were given CP 8 mg/kg intraperitoneally, while the N group was given NaCl 0.9% 1 mL/kg intraperitoneally. Result: Malondialdehyde (MDA) levels were found to be lower in P1-P8 groups compared to negative control group and P6 and P7 groups have levels equivalent to MDA levels of normal control group (p > 0.05). Conclusion: Combination of BSO and honey provides a protective effect on cisplatin-induced rat liver tissue damage indicated by reduced MDA levels, but all combination group showed antagonism effect.

scholarly journals PENGARUH PEMBERIAN EFEK JINTEN HITAM (NIGELLA SATIVA) TERHADAP APOPTOSIS TERHADAP SEL EPITEKL BRONKIOLUS DAN APOPTOSIS LIMFOSIT T SALURAN NAFAS BRONCHIOLUS PADA MODEL MENCIT ASTHMA

2012 ◽  
Vol 6 (2) ◽  
Author(s):  
Endang Sriwahyuni ◽  
IMAMA KHALIS NUR ARIFAH
Keyword(s):  
Mouse Model ◽  
Bronchial Asthma ◽  
Nigella Sativa ◽  
Negative Control Group ◽  
Positive Control ◽  
Negative Control ◽  
Seed Extract ◽  
Positive Control Group ◽  
Control Group ◽  
Black Seed

Abstract   Airway remodelling refers to the structural changes that occur in the airway wall in asthma.  These is  irreversible changes to the airway, with damage of bronchiolus epithelium because the  airway epithelium undergoes apoptosis  as one of its manifestations. Empirically black seed has been known as bronchial asthma therapy for many years ago. The purpose of the research is to test the effect of black seed (Nigella sativa L.) extract to apoptosis of bronchiolus epithelium and apoptosis of T lymphocytes the airway bronchial asthma on asthma mouse model. Female mice were used in the expeimental laboratory research and allergic mouse model was got by given ovalbumin twice within 3 weeks intraperitoneally and 3 times per week within 6 weeks by inhalation. Black seed extract is given in 3 different dose (0,024 cc/day, 0,048 cc/day and 0,096 cc/day) for 9 weeks. Sample was chosen randomly to share it within 5 group containing 6 mice in every group ; negative control group (without any treatment, n=6), positive control group (sensitizied with ovalbumin, n=6), JH1 group (sensitizied with ovalbumin and treating with first dose of black seed extract, n=6), JH2 group (sensitizied with ovalbumin and treating with second dose of black seed extract, n=6), JH3 group (sensitizied with ovalbumin and treating with third dose of black seed extract, n=6). The parametric that is used in this research is the change in the color of the nucleus of bronchiolus epithelium and T lymphocytes the airway bronchial asthma shown the occurrence of browning apoptosis. The result shown a significant increase of apoptosis of bronchiolus epithelium on  positive control group to compare with negative control group. The apoptosis of bronchiolus  epithelium on JH1 shown an increase compare with positive control group. For JH2 and JH3 groups shown a decrease the apoptosis of bronchial epithelium compare with positive control group. And the research indicate that the unsignificant aptosis decrease were happen on  a group with positive control instead of the group with negative control. Meanwhile, on JH1 group, apoptosis decrease were happening more significantly than the positive control group does, the aptosis increases were happen on JH2 and JH3 group, but its not capable to exceeding the positive control group apoptosis amount The conclusions is the black seed (Nigella sativa L.) extract can decrease apoptosis of bronchiolus epthelium on asthma mouse model in the third dose as the best result and the doses of black seed extract that were giving to the experiment mice can not increasing the apoptosis T Lymphocyte, thus requiring further research to figure out the perfect doses to increasing against respiratory tract apoptosis T Lymphocyte.   Keyword: asthma, black seed, the apoptosis of bronchiolus epithelium, apoptosis limfosit T.

scholarly journals PENGARUH EKSTRAK ETANOL BUAH LABU SIAM (SECHIUM EDULE JACQ.SWARTZ.) TERHADAP KADAR INTERLEUKIN 6 DAN HISTOPATOLOGI PANKREAS MENCIT HIPERGLIKEMIA YANG DIINDUKSI STREPTOZOTOCIN (STZ)

2017 ◽  
Vol 2 (1) ◽  
pp. 60-64
Author(s):  
Paska Ramawati Situmorang
Keyword(s):  
Interleukin 6 ◽  
Descriptive Analysis ◽  
Ethanol Extract ◽  
Negative Control Group ◽  
Positive Control ◽  
Negative Control ◽  
Control Group ◽  
Mice Model ◽  
Sechium Edule ◽  
Size Ofthe

Diabetes mellitus now becomes a global serious theat with continues encreasingly prevalence. Ethanol extract of squash fruit Chayotte (Sechium edule) is a plant that contains flavonoidsareanti-inflammatory. The objective of this study was to determine the effect of ethanol extract of Squash fruit Chayotte (Sechium edule) to Interleukin 6 level and increasedthe size ofthe diameter of the pancreas in STZ-induced hyperglycemic mice model. Goals : This research is experimental research uses Post test randomized controlled group design upon male mice, and divided into 4 groups; negative control group, positive control group is treated with streptozotocin at a dose of 60 mg/kg weight, and groups of therapy (dosage 100 gram/kg weight, 200 gram/kg weight). Methods : The measurement of the level interleleukin 6 (IL-6) used ELISA method and the description of histopatoligy pancreas. The data analysis of level IL-6 level uses One Way ANOVA (α = 0,005) and pancreas histology image used descriptive analysis. Result : The results showed a decrease in the levels of IL-6, although not significant if it’s compared to the positive control. The preparat histopathology pancreas observation increased diameter which showed widening of cell β of pancreas. Conclution : The conclusion of this research was that ethanol extract of squash fruit can reduce levels IL-6 and prevent the hiperglikemia mice pancreas damages

scholarly journals Spirulina platensis Extract Reduces Serum TNF-a, Neutrophils, and Increases Macrophage Count in Skin Incisional Mice Model

2020 ◽  
Vol 4 (2) ◽  
Author(s):  
Riski Dwi Utami ◽  
Tri Nur Kristina ◽  
Renni Yuniati
Keyword(s):  
Spirulina Platensis ◽  
Diclofenac Sodium ◽  
Negative Control Group ◽  
Positive Control ◽  
Negative Control ◽  
Control Group ◽  
M2 Macrophages ◽  
Therapeutic Group ◽  
Mice Model ◽  
Anti Inflammatory

Spirulinasp.is an alga that has antioxidant and anti-inflammatory potential.Phycocyanin contained in Spirulina platensis can reduce tumor necrosis factor-? levels, neutrophils, and M2 macrophages in inflammation. This research aims to investigate the anti-inflammatory activity of S. platensisin a mice wound model. Thirty-two male Wistar rats were incised and divided into 4 groups. The first therapeutic group (X1) received S. platensisextract at a dose of 500 mg/kgBW/day, and the second therapeutic group received S. platensisextract at a dose of 750 mg/kgBW/day. The negative control group (C1) received a saline solution and the positive control group (C2) received diclofenac sodium 20mg/kgBW/day. Serum TNF-? levels examined by the Enzyme-Linked Immunosorbent Assaymethod. Neutrophils and M2 macrophages were calculated by tissue biopsy and hematoxylin-eosin (HE) staining. Data analysis was performed with ANOVA test and LSD Post-Hoc Test. The average levels of serum TNF-? on the 14-day were 394.50; 180.33; 2,980.33; and 607.42 pg/ml for X1, X2, C1 and C2, respectively. The average neutrophils number on the 14-day were 8.00; 6.83; 14.67; and 11.17 for X1, X2, C1 and C2, respectively. The average macrophage number in the 14-day was 15.50; 19.17; 6.33; and 11.17 for X1, X2, C1 and C2, respectively. We found significant differences between TNF-? levels, the number of neutrophils, and the number of M2 macrophages. The administration of S. platensisextract at a dose of 750 mg/ kgBW/day reduces serum TNF-? levels, neutrophil count, and increases M2 macrophages in skin incisional mice model.

Evaluation of the Effectiveness of Cumin (Nigella Sativa) Seed Oil Extract as an Alternative Disinfectant of Dental Surfaces

2021 ◽  
Author(s):  
Mohammadreza Moaddeli ◽  
Abdolmehdi Araghizadeh ◽  
Ehsan Shabani
Keyword(s):  
Seed Oil ◽  
Nigella Sativa ◽  
Colony Count ◽  
Control Group ◽  
Bacterial Colony ◽  
Nigella Sativa Oil ◽  
Significant Difference ◽  
Oil Extract ◽  
Petri Dishes ◽  
Nigella Sativa Seed

Introduction: Cumin (Nigella Sativa) seed oil extract has some ingredients which have antimicrobial effects. The essential oils present in cumin act as antimicrobial agent and it influence on different type of Gram-negative and Gram-positive bacteria and also viruses, parasites and fungi. This study aimed to investigate the antimicrobial properties of cumin extract in disinfecting dentistry surfaces. Material and Methods: This study was performed experimentally and had three groups of cumin extract, Deconex and control group. For each of these groups, 12 culture media were prepared and we counted the colonies created in 24 hours and 48 hours and significance level was assessed using SPSS software and t-test.    Results: At 24 hours, there was a significant difference between the bacterial colony counts of the petri dishes from Cumin Seed (Nigella Sativa) Oil Extract at 5.83 and the Deconex at 0. And at 48 hours, there was also a significant difference since the bacterial colony count on the petri dishes with Cumin (Nigella Sativa) Oil Extract was too many to count and a 0.83 bacterial colony count for the petri dishes with the Deconex. Conclusion: The Cumin (Nigella Sativa) seed oil extract is not suitable to use as an alternative disinfectant of dental surfaces lonely. But some of its ingredients such as thymoquinone and hydroquinone can be used to produce a disinfecting solution.

scholarly journals Escalating Dose Antigen Specific Therapy with dsDNA Injection Regulate Balance Ratio of Inflammatory Cells in Pristane-Induced Lupus Mice Model

2019 ◽  
Vol 3 (1) ◽  
Author(s):  
Sri Poeranto
Keyword(s):  
Dendritic Cells ◽  
Inflammatory Cells ◽  
Negative Control Group ◽  
Positive Control ◽  
Negative Control ◽  
Th2 Cells ◽  
Control Group ◽  
Mice Model ◽  
Specific Therapy ◽  
Self Antigen

Immunosuppressant and steroid therapy for SLE have not shown satisfactory results. Another method of therapy that is being developed is vaccines and escalating dose immunotherapy using self-antigen. The aim of this study was to assess the balance of immune cells through the ratio of pro-inflammatory and anti-inflammatory cells and cytokines in SLE using self-antigen dsDNA therapy. Methods: Female Balb/c mice 6-8 weeks old separated randomly to negative control group and pristane induced lupus (PIL) mice group. PIL mice groups were injected pristane intraperitoneally. Twelve weeks after the injection, the mice were evaluated for clinical and serological manifestations (anti-dsDNA levels). Mice with lupus signs were divided into four groups; positive control group: PIL mice without EDI dsDNA therapy, treatment A: PIL mice with EDI dsDNA therapy dose I (0.01μg/ml, 0.1μg/ml, 1μg/ml), treatment B: PIL mice with EDI dsDNA therapy dose II (0.1μg/ml, 1μg/ml, 10μg/ml), and treatment C: PIL mice with EDI dsDNA therapy dose III (1μg/ml, 10μg/ml, 100μg/ml). dsDNA were injected once a week and the dose was increased every week. Samples were analyzed for active/inactive dendritic cells ratio, Th1/Th2 cells ratio, Th17/Treg cells ratio and IL-17/TGF-β levels ratio. Results: Escalating dose antigen specific therapy with dsDNA injection of third dose reduced active/inactive dendritic cells ratio (p=0.000), Th1/Th2 cells ratio (p=0.010), Th17/Treg ratio (p=0.004) and decrease IL-17/TGF- β levels ratio (p=0.004) significantly compared to positive control. Conclusion: Escalating dose antigen specific therapy with dsDNA injection of dose III was able to regulate balance ratio of inflammatory cells and cytokines in PIL mice thus the immune tolerance may improve compared to control groups.

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