OBSERVATIONS OF THE EFFECTS OF 17α-METHYLTESTOSTERONE (MT) ON THE GONADS, TESTES OF Clarias gariepinus BROODSTOCKS AFTER HORMONE-TREATED FEEDING TRIAL

A feeding trial was conducted to determine the effect of biscuit waste meal on the growth performance and utilization of Clarias gariepinus juveniles. A total of 300 juveniles of average weight 8.85g were randomly divided into five Treatments, each Treatment had three replicates. Twenty juveniles were distributed into each happa (0.7m3). The happa were suspended to ¾ of their volume using kuralon ropes carefully tied round the bamboo poles across the concrete tanks. Five diets containing 40% crude protein were formulated in which maize was replaced with biscuit waste meal at treatment; Diet 1 (TD1) 0%, 25% (TD2), 50% (TD3), 75% (TD4), 100% (TD5) levels. The fish were fed at 3% body weight per day for 10weeks. The results showed that biscuit waste was most suitable as an energy supplement when incorporated at 25% replacement (TD2) with maize. TD1 had the highest weight gain followed by TD2, TD3, TD4 and TD5 respectively. There were no significant differences (P>0.05) in the growth response in TD1 (0%), TD2 (25%) and TD3 (50%). It is therefore concluded that biscuit waste meal is a cheap source of non conventional energy source which can be used favorably to replace maize (25% inclusion level) as an energy source in the diets of C. gariepinus.

Download Full-text

PENAMBAHAN VITAMIN C PADA IKAN LELE DUMBO (Clarias gariepinus) UNTUK MENINGKATKAN TANGGAP KEBAL TERHADAP VAKSIN Aeromonas hydrophilia

Jurnal Perikanan Universitas Gadjah Mada ◽

10.22146/jfs.8846 ◽

1996 ◽

Vol 1 (1) ◽

pp. 35

Author(s):

Alim Isnansetyo

Keyword(s):

Growth Rate ◽

Vitamin C ◽

Clarias Gariepinus ◽

Challenge Test ◽

Fresh Water Fish ◽

Treatment Combination ◽

Feeding Trial ◽

Water Fish ◽

Aeromonas Hydrophilia ◽

High Result

Motile Aeromonas Septicimia is a dangerous disease on fresh water fish especially walking catfish and carp. Vaccination against the disease has been developed in Indonesia. However, there were high result variations of the vaccination. Immune response is influenced by level of vitamin C dietery. Objective of this experiment was to know effect of vitamin c levels on walking catfish’s specific immune respose. The levels of vitamin (0, 50, 500, and 1000 mg ascorbic acid (AA)/kg feed) were given to the fish. Immersion vaccination for 30 minutes (108 sel/ml) was carried out 15 days after feeding trial. Challenge test was conducted by injection of 0,2 ml bacteria suspension (LD80 concentration) was carried out 15 days after vaccination. Growth rate of the fish was observed monthly. Result of this experiment indicated that mortality in the challenge test due to A. hydrophila infection considerably decreased for treatment combination among vaccination; and 500 and 1000 mg AA/kg feed. These AA levels without vaccination could not reduce mortality.

Download Full-text

Histopathological changes in liver and kidney of sharptooth catfish fed on cooked Jatropha curcas seedmeal based diets

Savannah Veterinary Journal ◽

10.36759/svj.2020.073 ◽

2020 ◽

pp. 52-59

Keyword(s):

Jatropha Curcas ◽

Standard Procedure ◽

Control Diet ◽

Clarias Gariepinus ◽

Average Weight ◽

Test Diet ◽

Histopathological Changes ◽

Feeding Trial ◽

Liver And Kidney ◽

Dietary Treatments

Introduction: Histology of the organs of fish species is important in the understanding of the pathological changes related to nutritional sources. This study evaluates the histopathological alteration in the liver and kidney of sharptooth catfish fed diets containing Jatropha curcas seedmeal in a 56-day feeding trial. Methods: Clarias gariepinus fingerlings of average weight 2.61±0.02g were acclimatized for a week, and allotted into five dietary treatments; containing 0, 25, 50, 75 and 100% Jatropha curcas seedmeal replacement levels for soybean meal respectively. Each treatment was replicated three times with fifteen fish per replicate. Fish were fed 5% body weight on two equal proportions per day. Histological assessment was conducted using standard procedure. Results: There were no lesions on the photomicrographs of the kidney of fish exposed to all the dietary treatments while moderate vacuolation of the hepatocytes was observed in the liver of fish fed diet D1-control diet. There were very prominent melanomicrophage centres in the liver of Clarias gariepinus fed test diet D3 with some hepatocytes which appeared vacuolated. The trend of vacuolation of hepatocytes of the liver among some treatments were not dietary related as no visible lesions were seen in the liver of fish fed test diets D2 ; D4 and D5. Significance: This study established that Jatropha curcas based diets exert hepatoprotective effect on the liver of fish fed with the diets.

Download Full-text

Comparative Study on the Natural and Synthetic Hormones ofClarias gariepinus Broodstock

Nigerian Journal of Biotechnology ◽

10.4314/njb.v38i1.5 ◽

2021 ◽

Vol 38 (1) ◽

pp. 48-54

Author(s):

L.U. Onyia ◽

H.D. Ali ◽

H.A. Bello ◽

E.C. Onyia ◽

M. Musa

Keyword(s):

Standard Procedure ◽

Clarias Gariepinus ◽

Survival Rates ◽

Fertilization Rate ◽

Left Lobe ◽

Pituitary Extract ◽

Feeding Trial ◽

Synthetic Hormones ◽

Right Lobe ◽

The Right

The study was carried out for six (6) weeks at the Department of Fisheries Teaching and Research farm, Modibbo Adama University, Yola, to compare the reproductive performance of Clarias gariepinus using synthetic (ovaprim) and natural (C. gariepinus pituitary extract) hormones . At the end of the feeding trial, the fecundity, percentage fertilization, hatchability, and survival rates of the hatchlings were estimated using standard procedure. The hatchlings were further raised for six weeks and the increase in weight was determined. The results showed that female broodstock injected with C. gariepinus pituitary extract (CgPE) had a higher fecundity (124,000) while the female injected with Ovaprim had 65,000 eggs. The weight of the testes significantly differed (p<0.05) across the treatments, the right lobe weighed 1.63g while the left lobe weighed 1.98g . Subsequently, a higher fertilization rate was recorded in CgPE (91.1%) while Ovaprim had 85.55% . However, Ovaprim had a higher percentage hatchability of 82.93% while CgPE had 82.73% . At the end of the six (6) weeks feeding period, the survival rate was higher in the fish treated with CgPE (82.31%) while the fish injected with Ovaprim had 79.68% . The research exposed the efficacy of using CgPE in the absence of synthetic hormone, CgPE is a preferred substitute that Hatchery managers can utilize for fingerlings production.

Download Full-text

Oral nutritional supplement for children with cancer: 7 different high-energy drinks compared to cacao, a double-blinded feeding trial

10.1055/s-0038-1647233 ◽

2018 ◽

Cited By ~ 1

Author(s):

L Tenius ◽

H Buchner ◽

H Bauer ◽

I Schmid

Keyword(s):

Energy Drinks ◽

Nutritional Supplement ◽

High Energy ◽

Feeding Trial ◽

Children With Cancer ◽

Oral Nutritional Supplement ◽

Double Blinded

Download Full-text

Novel herbal veterinary narcotics for aquacultures of Clarias gariepinus

10.1055/s-0039-3399735 ◽

2019 ◽

Author(s):

K Kuchta ◽

W Waser ◽

S Cameron ◽

H Rausch

Keyword(s):

Clarias Gariepinus

Download Full-text

EFEKTIVITAS ANTIBAKTERI BAWANG PUTIH (Allium sativum L) SEBAGAI PENGAWET ALAMI PADA IKAN LELE DUMBO (Clarias gariephinus) SEGAR

Surya Medika: Jurnal Ilmiah Ilmu Keperawatan dan Ilmu Kesehatan Masyarakat ◽

10.32504/sm.v14i1.106 ◽

2019 ◽

Vol 14 (1) ◽

pp. 26

Author(s):

Dyah Anggraeni ◽

Nurlela Nurlela

Keyword(s):

Room Temperature ◽

Allium Sativum ◽

Research Result ◽

Clarias Gariepinus ◽

Storage Period ◽

The Body ◽

Garlic Extract ◽

African Catfish ◽

Natural Preservatives ◽

Colony Counter

Background: Natural preservatives are compounds produced by natural ingredients that can suppress bacterial growth and development. Natural preservatives are carried out because most of the preservatives circulating are chemicals and unsafe for the body. One of the natural preservatives is by using garlic extract (Allium sativum L). Objective: This study is aimed to determine the effectiveness of the antibacterial garlic (Allium sativum L) as a natural preservative in fresh African catfish (Clarias gariepinus). Method: This research used the Pour Plate iroculation method. African catfish (Clarias gariepinus) which is soaked with garlic (Allium sativum L) with a concentration of 7%, 14% and 21% for 30 minutes, then the fish will be kept at room temperature with a storage period of 24 hours and 48 hours and calculated growth in bacterial numbers with the Colony counter. Result: Based on the research result, it was found that garlic extract (Allium sativum L) can obstruct the effectiveness of antibacterial in African catfish (Clarias gariepinus) at a concentration of 14%.

Download Full-text

PHYTOCHEMICAL SCREENING AND LARVICIDAL EFFECT EVALUATION OF AZADIRACHTA INDICA (L) LEAF EXTRACT ON DERMESTES MACULATUS (DE GEER, 1774) INFESTATION OF SMOKED CLARIAS GARIEPINUS (BURCHELL, 1822)

FUDMA Journal of Sciences ◽

10.33003/fjs-2020-0402-89 ◽

2020 ◽

Vol 4 (2) ◽

pp. 41-45

Author(s):

Sadam Sulaiman Indabo ◽

Rufai Zakari

Keyword(s):

Azadirachta Indica ◽

Leaf Extract ◽

Standard Procedure ◽

Clarias Gariepinus ◽

Phytochemical Screening ◽

Range Finding ◽

Dermestes Maculatus ◽

Randomized Design ◽

Fish Samples ◽

Initial Source

Dermestes maculatus is a major pest of stored fish in Nigeria, fish is preserved with highly persistent synthetic chemicals. There have been an increasing effort at developing plant-based toxicants that are environmentally friendly. Therefore, this study was conducted to screen the leaf extract of Azadirachta indica for phytochemical constituents. The efficacy of the methanolic leaf extract of the plant against D. maculatus was also evaluated. The leaves of A. indica were obtained, processed and taken to laboratory for methanolic extraction. Phytochemical screening was carried out to identify saponins, flavonoids, alkaloids, tannins, steroids, cardiac glycosides, glycosides, triterpenes and carbohydrates using standard procedure. Both clean and infested samples of smoke-dried Clarias gariepinus were purchased from Sabon Gari Market, Zaria. The infested samples were used as initial source of D. maculatus larvae. Range finding test was carried out prior to the bioassay. During the bioassay, clean un-infested fish samples were weighed and introduced into kilner jars and respective dosages of 0.2g, 0.4g, 0.6g and 0.8g of extract were added. Another set of fish samples without the extract were maintained as control. The experiment was arranged in Completely Randomized Design with three replicates. Fifteen larvae of D. maculatus were introduced into both treated and control containers and covered. Mortality was recorded after 24, 48, 72 and 96 hours. Data obtained revealed an increase in mortality with increasing dose and time. Mortality ranged from 1.67 to 11.00. Therefore, leaf extract of A. indica should be incorporated in the development of larvicide against D. maculatus.

Download Full-text

PEMBEKUAN SEMEN LELE DUMBO (Clarias Gariepinus BURCHELL 1822) SEBAGAI MODEL KRIOPRESERVASI SEMEN IKAN

Jurnal Natural ◽

10.30862/jn.v9i1.778 ◽

2010 ◽

Vol 9 (1) ◽

Author(s):

Lutfi Lutfi

Keyword(s):

Liquid Nitrogen ◽

Clarias Gariepinus ◽

African Catfish ◽

Nitrogen Vapor ◽

Dmso Concentration ◽

Freezing Temperatures ◽

Vapor Liquid

Effect of four types of diluentss and four concentration of DMSO (5%, 10%, 15% and 20%) against the motility of African catfish sperm were evaluated after storage at freezing temperatures. The steps in preparation the 16 treatments combination of the diluents are preparation of diluents, mixing diluents with DMSO, packing of semen into 0.3-ml straw, equilibration of semen at 4 °C for 30 minutes, freezing of semen in nitrogen vapor liquid at a height of 6.5 cm for 10 minutes, and subsequently storage of semen in liquid nitrogen (-196 0C) for further analysis of post-thawing motility (PTM). The result showed that the highest level of motility of spermatozoa was in treatment P1D15 (45.7 ± 4.3%) and the lowest was in treatment P2D20 (14.5 ± 13.2%). The best diluent in this observation was diluents containing NaCl, KCl, CaCl2 and NaHCO3. The best concentration was DMSO 15%. While the best interaction between the concentration of DMSO diluents is P1D15 treatments containing NaCl, KCl, CaCl 2 and NaHCO3 with a combination of 15% DMSO concentration. The conclusion of the research is that diluents containing NaCl, KCl, CaCl2 and NaHCO3 with a combination of 15% DMSO concentration can be used in cryopreservation of African catfish semen.

Download Full-text

Pengaruh ovaprim, aromatase inhibitor, dan hipofisa terhadap kualitas telur ikan Lele Dumbo (Clarias gariepinus)

e-Journal BUDIDAYA PERAIRAN ◽

10.35800/bdp.5.1.2017.14836 ◽

2017 ◽

Vol 5 (1) ◽

Author(s):

Erwin A. Aziz ◽

Ockstan Kalesaran

Keyword(s):

Survival Rate ◽

Experimental Design ◽

Aromatase Inhibitor ◽

Fish Larvae ◽

Clarias Gariepinus ◽

Randomized Design ◽

Completely Randomized Design ◽

The Difference ◽

Hatching Eggs

This study aimed to determine the effect of ovaprim hormone, aromatase inhibitor and pituitary on the quality of the catfish eggs (Clarias gariepinus). Experimental Design used was Completely Randomized Design (CRD) with four treatments, each with three replications. Treatment A: ovaprim; treatment B: Aromatase inhibitors, treatment C: hypophysis and treatment D: Control. The results showed that the difference in treatment gave highly significant effect on fertilization and hatching eggs but no significant effect on the survival rate of larvae. Aromatase inhibitor hormone was the best because it provided highly significant effect on fertilization (92.66%), hatchability of eggs (95%), and surviva rate (81.33%) of fish larvae. Keywords : Clarias gariepinus. Ovaprim, Aromatase Inhibitor, Hypophysis, egg, larvae

Download Full-text