scholarly journals Determination of antimicrobial activity of extracts from Melissa officinalis L.

2020 ◽  
Vol 100 (4) ◽  
pp. 79-84
Author(s):  
Nargiz Seidalina ◽  
◽  
Saule Ahmetova ◽  
Marlen Smagulov ◽  
Gayane Аtazhanova ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Bacillus Subtilis ◽  
Water Extract ◽  
Antimicrobial Effect ◽  
Antimicrobial Drugs ◽  
Gram Positive Bacteria ◽  
Weak Activity ◽  
First Time ◽  
Yeast Fungus

The constant growth of antibiotic resistance of bacteria creates the need for the development of antimicrobial drugs from extracts of medicinal plants. For the first time, the article presents the results of antimicrobial activity of extracts of Melissa officinallis L. (drug melissa) obtained by various methods, including ultrasonic extraction. The antimicrobial activity of the samples was studied in relation to strains of gram-positive bacteria Staphylocococus aureus, Bacillus subtilis, gram-negative strains Escherichia coli and yeast fungus Candida albicans. It was found that samples of aqueous extracts of drug melissa obtained by infusion and extraction by ultrasound have a pronounced antimicrobial effect on Staphylococus aureus, ultrasonic extracts of 30 % — and 90 % — alcohol extracts and water extract are active on Staphylocococus aureus and Bacillus subtilis and all samples showed weak activity The obtained data can be used for development of new phytopreparations with expressed antimicrobial activity.

scholarly journals Evaluation of Antimicrobial Activity of Acacia etbaica Water Extract Leafs against Some Pathogenic Microorganisms

2021 ◽  
Vol 2 (11) ◽  
pp. 1132-1135
Author(s):  
Abdulmageed B Abdullah ◽  
Abdulbaki Al-zaemey ◽  
Rasheed Hasan Mudhesh Al-Husami ◽  
Mofeed Al-Nowihi
Keyword(s):  
Antimicrobial Activity ◽  
Candida Albicans ◽  
Water Extract ◽  
Antimicrobial Effect ◽  
Inhibition Zone ◽  
Gram Negative Bacteria ◽  
Gram Positive ◽  
E Coli ◽  
Gram Positive Bacteria ◽  
Inhibition Zones

The antimicrobial activity about water extract of Acacia etbaica was examined by using agar well diffusion methods against five gram-positive and negative bacteria[Staphylococcus aureus (ATCC653-8), Pseudomonas aeruginosa MTCC2453, Bacillus cereus (ATCC6633), Escherichia coli MTCC739, and one local isolate (Staphylococcus epidermides)] in addition to Candida albicans (ATCC2019). this results designated that the water extract of Acacia etbaica possess antimicrobial efficacy against all tested microorganisms either (gram-positive and negative bacteria) or fungi (Candida albicans).Whereas the gram-positive bacteria (S. aureus, S. epidermides and B. cereus) with inhibition zones (21, 19.5 and 16.5) respectively was more sensitive than gram-negative bacteria (P. aeruginosa and E. coli) and C. albicans with inhibition zones 16 and 14.5 respectively. The antimicrobial effect was directly proportional with concentration of water extract where the highest inhibition zone at higher concentration 30% equal 21mm against S.aureus and the lower inhibition zone at lower concentration 5% equal 10mm against C. albicans...

Antimicrobial Activity of Isopteropodine

2005 ◽  
Vol 60 (5-6) ◽  
pp. 385-388 ◽  
Author(s):  
Rubén García ◽  
Cesia Cayunao ◽  
Ronny Bocic ◽  
Nadine Backhouse ◽  
Carla Delporte ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Antibacterial Activity ◽  
Gram Positive ◽  
Uncaria Tomentosa ◽  
Gram Positive Bacteria

Bioassay-directed fractionation for the determination of antimicrobial activity of Uncaria tomentosa, has led to the isolation of isopteropodine (0.3%), a known Uncaria pentacyclic oxindol alkaloid that exhibited antibacterial activity against Gram positive bacteria.

scholarly journals The Search for Biologically Active Compounds in the Series of N-ethoxyethylpiperidine Derivatives

2019 ◽  
Vol 21 (2) ◽  
pp. 125
Author(s):  
U.B. Issayeva ◽  
G.S. Akhmetova ◽  
U.M. Datkhayev ◽  
M.T. Omyrzakov ◽  
K.D. Praliyev ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Biologically Active ◽  
Hydroxyl Group ◽  
Diffusion Method ◽  
Acylation Reaction ◽  
Gram Positive Bacteria ◽  
Cyclopropanecarboxylic Acid ◽  
Yeast Fungus ◽  
Acid Esters

With the aim to introduce fragment of cyclopropane and fragments of p-, m-, o-fluorophenyls into the structures of N-ethoxyethylpiperidines, acylation of oxime and phenylacetylenic alcohol of 1-(2-ethoxyethyl)-4-ketopiperidine by cyclopropanecarbonylchloride was carried out; on the basis of 1-(2-ethoxyethyl)-4-ethynyl-4-hydroxypiperidine (cascaine alcohol), acylation by 4-fluoro-, 3-fluoro-, 2-fluorobenzoylchlorides was carried out with formation of the corresponding piperidine containing hydrochlorides of cyclopropanecarboxylic acid esters and para-, meta-, ortho-fluorobenzoic esters. Acylation reaction on the hydroxyl group of compounds is carried out in absolute dioxane, the acylating agents are cyclopropanecarbonylchloride, p-, m-, o-fluorobenzoyl chlorides taken in excess. The obtained esters of cyclopropanecarboxylic and para-, meta-, ortho-fluorobenzoic acids are crystalline substances with a clear melting point, well soluble in water, ethanol, acetone. P-fluorobenzoates are obtained with better yields, m-fluorobenzoates occupy an intermediate position, and o-fluorobenzoates are formed with the lowest yields. The best yields of fluorobenzoates are obtained using dioxane as a solvent. Para-, meta-, ortho-fluorobenzoic esters of 1-(2-ethoxyethyl)-4-ethynyl-4-hydroxypiperidine coded A-4 – A-6 were studied for the presence of antimicrobial activity, the actions of these preparations were evaluated in vitro in relation to strains of gram-positive bacteria Staphylococcus aureus, Bacillus subtilis, gram-negative strains of Escheriсhia coli, Pseudomonas aeruginosa and to yeast fungus Сandida albicans by the diffusion method into agar (holes). Introduction of fluorine atom into the structure of cascaine lead to manifestation of antimicrobial activity.

scholarly journals Chemical Compositions and Antimicrobial Activity of the Essential Oils of Piper abbreviatum, P. erecticaule and P. lanatum (Piperaceae)

2014 ◽  
Vol 9 (12) ◽  
pp. 1934578X1400901 ◽  
Author(s):  
Wan Mohd Nuzul Hakimi Wan Salleh ◽  
Farediah Ahmad ◽  
Khong Heng Yen
Keyword(s):  
Antimicrobial Activity ◽  
Essential Oils ◽  
Antimicrobial Agents ◽  
Antimicrobial Activities ◽  
Dilution Method ◽  
Chemical Compositions ◽  
Gram Positive Bacteria ◽  
Aerial Parts ◽  
Ms Analysis ◽  
Weak Activity

The study was designed to examine the chemical composition and antimicrobial activities of essential oils extracted from the aerial parts of three Piper species: Piper abbreviatum, P. erecticaule and P. lanatum, all from Malaysia. GC and GC/MS analysis showed qualitative and quantitative differences between these oils. GC and GC-MS analysis of P. abbreviatum, P. erecticaule and P. lanatum oils resulted in the identification of 33, 35 and 39 components, representing 70.5%, 63.4% and 78.2% of the components, respectively. The major components of P. abbreviatum oil were spathulenol (11.2%), ( E)-nerolidol (8.5%) and β-caryophyllene (7.8%), whereas P. erecticaule oil mainly contained β-caryophyllene (5.7%) and spathulenol (5.1%). Borneol (7.5%), β-caryophyllene (6.6%) and α-amorphene (5.6%) were the most abundant components in P. lanatum oil. Antimicrobial activity was carried out using disc diffusion and broth micro-dilution method against nine microorganisms. All of the essential oils displayed weak activity towards Gram-positive bacteria with MIC values in the range 250–500 μg/mL. P. erecticaule oil showed the best activity on Aspergillus niger (MIC 31.3 μg/mL), followed by P. lanatum oil (MIC 62.5 μg/mL). This study demonstrated that the essential oils have potential as antimicrobial agents and may be useful in the pharmaceutical and cosmetics industries.

Screening of Antimicrobial Activity and Cytotoxic Effects of Two Cladonia Species

2013 ◽  
Vol 68 (5-6) ◽  
pp. 191-197 ◽  
Author(s):  
Birkan Açıkgöz ◽  
İskender Karaltı ◽  
Melike Ersöz ◽  
Zeynep M. Coşkun ◽  
Gülşah Çobanoğlu ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Antimicrobial Activities ◽  
Chloroform Extract ◽  
Cytotoxic Effects ◽  
Minimal Inhibitory Concentrations ◽  
E Coli ◽  
Methanol Extracts ◽  
Gram Positive Bacteria

The present study explores the antimicrobial activity and cytotoxic effects in culture assays of two fruticose soil lichens, Cladonia rangiformis Hoffm. and Cladonia convoluta (Lamkey) Cout., to contribute to possible pharmacological uses of lichens. In vitro antimicrobial activities of methanol and chloroform extracts against two Gram-negative bacteria (Pseudomonas aeruginosa and Escherichia coli), two Gram-positive bacteria (Enterococcus faecalis and Staphylococcus aureus), and the yeast Candida albicans were examined using the paper disc method and through determination of minimal inhibitory concentrations (MICs). The data showed the presence of antibiotic substances in the chloroform and the methanol extracts of the lichen species. The chloroform extracts exhibited more signifi cant antimicrobial activity than the methanol extracts. However, a higher antifungal activity was noted in the methanol extract of C. rangiformis. The maximum antimicrobial activity was recorded for the chloroform extract of C. convoluta against E. coli. The cytotoxic effects of the lichen extracts on human breast cancer MCF-7 cells were evaluated by the trypan blue assay yielding IC50 values of ca. 173 and 167 μg/ml for the extracts from C. rangiformis and C. convoluta, respectively.

scholarly journals Fungi Treated with Small Chemicals Exhibit Increased Antimicrobial Activity against Facultative Bacterial and Yeast Pathogens

2014 ◽  
Vol 2014 ◽  
pp. 1-13 ◽  
Author(s):  
Christoph Zutz ◽  
Dragana Bandian ◽  
Bernhard Neumayer ◽  
Franz Speringer ◽  
Markus Gorfer ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Antimicrobial Effect ◽  
Small Mass ◽  
Antimicrobial Compounds ◽  
Antimicrobial Drugs ◽  
Antimicrobial Substances ◽  
Log Reduction ◽  
Penicillium Restrictum ◽  
Test Organisms ◽  
Recent Sequencing

For decades, fungi have been the main source for the discovery of novel antimicrobial drugs. Recent sequencing efforts revealed a still high number of so far unknown “cryptic” secondary metabolites. The production of these metabolites is presumably epigenetically silenced under standard laboratory conditions. In this study, we investigated the effect of six small mass chemicals, of which some are known to act as epigenetic modulators, on the production of antimicrobial compounds in 54 spore forming fungi. The antimicrobial effect of fungal samples was tested against clinically facultative pathogens and multiresistant clinical isolates. In total, 30 samples of treated fungi belonging to six different genera reduced significantly growth of different test organisms compared to the untreated fungal sample (growth log reduction 0.3–4.3). For instance, the pellet ofPenicillium restrictumgrown in the presence of butyrate revealed significant higher antimicrobial activity againstStaphylococcus(S.)aureusand multiresistantS. aureusstrains and displayed no cytotoxicity against human cells, thus making it an ideal candidate for antimicrobial compound discovery. Our study shows that every presumable fungus, even well described fungi, has the potential to produce novel antimicrobial compounds and that our approach is capable of rapidly filling the pipeline for yet undiscovered antimicrobial substances.

scholarly journals Composition and Antimicrobial Activity ofEuphrasia rostkovianaHayne Essential Oil

2015 ◽  
Vol 2015 ◽  
pp. 1-5 ◽  
Author(s):  
Pavel Novy ◽  
Hana Davidova ◽  
Cecilia Suqued Serrano-Rojero ◽  
Johana Rondevaldova ◽  
Josef Pulkrabek ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Chemical Composition ◽  
Essential Oil ◽  
Bacterial Infections ◽  
Antimicrobial Effect ◽  
Main Constituent ◽  
Hexadecanoic Acid ◽  
Eye Infections ◽  
Gram Positive Bacteria ◽  
Health Disorders

Eyebright,Euphrasia rostkovianaHayne (Scrophulariaceae), is a medicinal plant traditionally used in Europe for the treatment of various health disorders, especially as eyewash to treat eye ailments such as conjunctivitis and blepharitis that can be associated with bacterial infections. SomeEuphrasiaspecies have been previously reported to contain essential oil. However, the composition and bioactivity ofE. rostkovianaoil are unknown. Therefore, in this study, we investigated the chemical composition and antimicrobial activity of the eyebright essential oil against some organisms associated with eye infections:Enterococcus faecalis,Escherichia coli,Klebsiella pneumoniae,Staphylococcus aureus,S. epidermidis,Pseudomonas aeruginosa, andCandida albicans. GC-MS analysis revealed more than 70 constituents, with n-hexadecanoic acid (18.47%) as the main constituent followed by thymol (7.97%), myristic acid (4.71%), linalool (4.65%), and anethole (4.09%). The essential oil showed antimicrobial effect against all organisms tested with the exception ofP. aeruginosa. The best activity was observed against all Gram-positive bacteria tested with the minimum inhibitory concentrations of 512 µg/mL. This is the first report on the chemical composition ofE. rostkovianaessential oil and its antimicrobial activity.

Antimicrobial Activity of Fractions and Compounds from Calophyllum brasiliense (Clusiaceae/Guttiferae)

2004 ◽  
Vol 59 (9-10) ◽  
pp. 657-662 ◽  
Author(s):  
Juliana B. Pretto ◽  
Valdir Cechinel-Filho ◽  
Vânia F. Noldin ◽  
Mara R. K. Sartori ◽  
Daniela E. B Isaias ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Pathogenic Bacteria ◽  
Protocatechuic Acid ◽  
Inhibitory Concentration ◽  
Gram Negative Bacteria ◽  
Gram Positive ◽  
Gram Positive Bacteria ◽  
Methanolic Extracts ◽  
Calophyllum Brasiliense

Abstract Calophyllum brasiliense (Clusiaceae/Guttiferae) is a native Brazilian medicinal plant traditionally used against several diseases, including infectious pathologies. Crude methanolic extracts (CME) and two fractions, denoted non-polar (soluble in chloroform) and polar (nonsoluble in chloroform), were prepared from different parts of the plant (roots, stems, leaves, flowers and fruits) and studied. The following compounds were isolated and tested against pathogenic bacteria and yeasts by determination of the minimal inhibitory concentration (MIC): brasiliensic acid (1), gallic acid (2), epicatechin (3), protocatechuic acid (4), friedelin (5) and 1,5-dihydroxyxanthone (6). The results indicated that all the parts of the plant exhibited antimicrobial activity against Gram-positive bacteria, which are selectively inhibited by components of C. brasiliense. No activity was observed against Gram-negative bacteria and yeasts tested. Regarding the isolated compounds, substance 4 showed antimicrobial activity against all the tested microorganisms, whereas compound 6 exhibited antimicrobial activity only against Gram-positive bacteria. The results from the current study confirm and justify the popular use of this plant to treat infectious processes.

scholarly journals Antimicrobial Effect of Bacteriocin produced Pediococcus pentosaceus on some clinical isolates

2017 ◽  
Vol 27 (5) ◽  
pp. 26 ◽  
Author(s):  
Nehad A. Taher
Keyword(s):  
Antimicrobial Activity ◽  
Protein Concentration ◽  
Antimicrobial Effect ◽  
Clinical Isolates ◽  
Gram Negative Bacteria ◽  
Gram Negative ◽  
Pediococcus Pentosaceus ◽  
Gram Positive Bacteria ◽  
Agar Plug ◽  
Made In

About 10 isolates of Pediococcus sp were isolated from different cheese made in Iraq, These isolates were identified morphologically and biochemically and Api20 kit, thus there was only 6 isolate were identified as Pediococcus pentosaceus (60%).In this study, we investigate, the effect of crude Bacteriocin from Pediococcus pentosaceus on 30 clinical isolates (5 E.coli, 5 Klepsiella pneumoniae, 5 Staphylococcus aureus, 5 Pseudomonas aeroginosa, 5 Bacillus subtilis, 5 Candida albicans). The protein concentration of this Bacteriocin was measured 67mg\ml by Bradford method and used as (1:2) by vol during the measuring the antimicrobial activity against the above clinical isolates by two methods wells and  agar plug assay. The results showed that  the inhibitory activity of this Bacteriocin was higher by wells method than agar pluq assay against Gram–positive bacteria or Gram-negative bacteria and yeast under this study.

Quinolizidine Alkaloid Profiles of Lupinus varius orientalis, L. albus albus, L. hartwegii, and L. densiflorus

2001 ◽  
Vol 56 (1-2) ◽  
pp. 21-30 ◽  
Author(s):  
Assem El-Shazly ◽  
Abdel-Monem M. Ateya ◽  
Michael Wink
Keyword(s):  
Mass Spectrometry ◽  
Antimicrobial Activity ◽  
Bacillus Subtilis ◽  
New World ◽  
Indole Alkaloids ◽  
Quinolizidine Alkaloids ◽  
World Species ◽  
Capillary Glc ◽  
First Time ◽  
Alkaloid Profiles

Abstract Alkaloid profiles of two Lupinus species growing naturally in Egypt (L. albus albus [synonym L. term is], L. varius orientalis) in addition to two New World species (L. hartwegii, L. densiflorus) which were cultivated in Egypt were studied by capillary GLC and GLC-mass spectrometry with respect to quinolizidine alkaloids. Altogether 44 quinolizidine, bipiperidyl and proto-indole alkaloids were identified; 29 in L. albus, 13 in L. varius orientalis, 15 in L. hartwegii, 6 in L. densiflorus. Some of these alkaloids were identified for the first time in these plants. The alkaloidal patterns of various plant organs (leaves, flowers, stems, roots, pods and seeds) are documented. Screening for antimicrobial activity of these plant extracts demonstrated substantial activity against Candida albicans, A spergillus flavus and Bacillus subtilis.

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