Content of fatty acids in pig muscle tissue after application of feed additives LG-MAX and SEL-PLEX

The energy density of diets can be increased by the inclusion of fat containing long chain fatty acids which may have antimicrobial activity. Their interaction with antibiotic feed additives is unknown. This study was designed to investigate the effects of monensin sodium (Romensin - Elanco Products Ltd) an antibiotic feed additive, and supplementary fat (BP fat prills - BP Nutrition Ltd) when fed either alone or together in the diets of barley-beef bulls.

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Synbiotics impact on dominant faecal microbiota and short-chain fatty acids production in sows

FEMS Microbiology Letters ◽

10.1093/femsle/fnz157 ◽

2019 ◽

Vol 366 (13) ◽

Cited By ~ 2

Author(s):

Katarzyna Śliżewska ◽

Agnieszka Chlebicz

Keyword(s):

Fatty Acids ◽

Intestinal Microbiota ◽

High Performance ◽

Anaerobic Bacteria ◽

Short Chain Fatty Acids ◽

Feed Additives ◽

Short Chain ◽

Control Group ◽

The Impact ◽

Chain Fatty Acids

ABSTRACT The aim of this study was to estimate the influence of synbiotics on intestinal microbiota and its metabolism in sows. Three different synbiotics were administered with feed to animals from three experimental groups. Two groups of sows were given commercially available probiotics (BioPlus 2B®, Cylactin® LBC) as forage additives for comparison. The control group of sows was given unmodified fodder. The study was conducted for 48 days (10 days before farrowing, and continued 38 days after) and faeces samples were collected four times. The scope of this work was to designate the dominant microbiota in sows’ faeces. Therefore, the total number of anaerobic bacteria, Bifidobacterium sp., Lactobacillus sp., Bacteroides sp., Clostridium sp., Enterococcus sp., Enterobacteriaceae, Escherichia coli and yeast was determined, using the plate method. Changes in the concentration of lactic acid, short-chain fatty acids (SCFAs) and branched-chain fatty acids (BCFAs) were also determined in correlation with the feed additives administered to the sows using high-performance liquid chromatography analysis (HPLC). Our results allowed us to conclude that synbiotics have a beneficial effect on intestinal microbiota of sows and its metabolism. We observed that the impact of the synbiotics on the microbiota was more significant than the one induced by probiotics.

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Comparative Analysis of Content of Omega-3 Polyunsaturated Fatty Acids in Food and Muscle Tissue of Fish from Aquaculture and Natural Habitats

Contemporary Problems of Ecology ◽

10.1134/s199542551803006x ◽

2018 ◽

Vol 11 (3) ◽

pp. 297-308 ◽

Cited By ~ 4

Author(s):

M. I. Gladyshev ◽

L. A. Glushchenko ◽

O. N. Makhutova ◽

A. E. Rudchenko ◽

S. P. Shulepina ◽

...

Keyword(s):

Fatty Acids ◽

Comparative Analysis ◽

Polyunsaturated Fatty Acids ◽

Muscle Tissue ◽

Omega 3 ◽

Natural Habitats

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Effects of plants containing secondary metabolites as feed additives on rumen metabolites and methanogen diversity of buffaloes

Animal Production Science ◽

10.1071/an15596 ◽

2016 ◽

Vol 56 (3) ◽

pp. 472 ◽

Cited By ~ 4

Author(s):

L. Samal ◽

L. C. Chaudhary ◽

N. Agarwal ◽

D. N. Kamra

Keyword(s):

Fatty Acids ◽

Lactic Acid ◽

Secondary Metabolites ◽

Dry Matter ◽

Volatile Fatty Acids ◽

Feed Additives ◽

Dry Matter Intake ◽

Control Group ◽

Rumen Ph ◽

Pooled Samples

Four fistulated adult Murrah buffaloes were fed on a basal diet consisting of wheat straw and concentrate mixture in a 4 × 4 Latin square design to study the effects of feeding plants containing secondary metabolites on rumen metabolites and methanogen diversity. The four groups were Control (no additive), Mix-1 (ajwain oil and lemon grass oil in a 1 : 1 ratio @ 0.05% of dry matter intake), Mix-2 (garlic and soapnut in a 2 : 1 ratio @ 2% of dry matter intake) and Mix-3 (garlic, soapnut, harad and ajwain in a 2 : 1 : 1 : 1 ratio @ 1% of dry matter intake). In each phase of 30 days’ duration, after 19 days of feeding, rumen liquor was sampled for two consecutive days at 0, 2, 4, 6 and 8 h post-feeding, whereas rumen content was sampled at 0 h feeding. The pH of the rumen liquor was recorded at every collection and then the rumen liquor of every collection was pooled day-wise and animal-wise. These pooled samples were used for estimation of rumen metabolites like ammonia, lactic acid and volatile fatty acids. Microscopic counting of protozoa was done in both 0 h and pooled samples of rumen liquor. Rumen contents collected from different locations of rumen were processed for enzyme estimation. The rumen contents were squeezed and the liquid portion was used for DNA isolation, which was further processed to determine methanogen diversity. Daily intake of feed was similar (P > 0.05) in all the four groups. The ammonia-N concentration and ciliate protozoa population were reduced significantly in the treatment groups supplemented with additives. Rumen pH, lactic acid, volatile fatty acids and enzyme activities were not affected (P > 0.05) by feeding of any of these additives. Methanogenic diversity comparison was made between the Control and Mix-1 group. The basic local alignment search tool (BLAST) analysis of the 133 (44 from the Control group and 89 from the Mix-1 group) sequences showed similarity of the sequences of rumen archaea by up to 97% to the known sequences of rumen methanogens. The sequences with minimum length of 750 bp were selected for phylogenetic analysis. Per cent identity of these sequences with that of the available nearest neighbour as calculated by MEGA 5.03 software showed identity of the clones in the range of 88–97%. The clones were similar with Methanobrevibacter smithii ATCC 35061, uncultured Methanobrevibacter sp. clone MEME95 and M. ruminantium M1. Overall, feeding of any of these feed additives to fistulated buffaloes did not affect feed intake, rumen pH, or rumen metabolites except ammonia and enzyme profile. Methanogen diversity showed the possibility of Methanobrevibacter as the major methanogen in buffalo rumen liquor.

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A sequence variant in the diacylglycerol O-acyltransferase 2 gene influences palmitoleic acid content in pig muscle

Scientific Reports ◽

10.1038/s41598-021-94235-z ◽

2021 ◽

Vol 11 (1) ◽

Author(s):

Emma Solé ◽

Roger Ros-Freixedes ◽

Marc Tor ◽

Ramona N. Pena ◽

Joan Estany

Keyword(s):

Fatty Acids ◽

Fatty Acid ◽

Palmitoleic Acid ◽

De Novo ◽

Positive Impact ◽

Subcutaneous Fat ◽

Sequence Variant ◽

Sequence Variants ◽

Triacylglycerol Synthesis ◽

Pig Muscle

AbstractThe bulk of body fat in mammals is in the form of triacylglycerol. Diacylglycerol O-acyltransferase 2 (DGAT2) catalyses the terminal step in triacylglycerol synthesis. The proximity of DGAT2 with stearoyl-CoA desaturase (SCD) in the endoplasmic reticulum may facilitate provision of de novo SCD-mediated fatty acids as substrate for DGAT2. Here, we first searched for sequence variants in the DGAT2 gene to then validate their effect on fat content and fatty acid composition in muscle, subcutaneous fat and liver of 1129 Duroc pigs. A single nucleotide polymorphism in exon 9 (ss7315407085 G > A) was selected as a tag variant for the 33 sequence variants identified in the DGAT2 region. The DGAT2-G allele increased DGAT2 expression in muscle and had a positive impact on muscular C14 and C16 fatty acids at the expense of C18 fatty acids. Although there was no evidence for an interaction of DGAT2 with functional SCD genotypes, pigs carrying the DGAT2-G allele had proportionally more palmitoleic acid relative to palmitic acid. Our findings indicate that DGAT2 preferentially uptakes shorter rather than longer-chain fatty acids as substrate, especially if they are monounsaturated, and confirm that fatty acid metabolism in pigs is subjected to subtle tissue-specific genetic regulatory mechanisms.

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Enhancing unsaturated fatty acids in ewe's milk by feeding rapeseed or linseed oil

Czech Journal of Animal Science ◽

10.17221/1704-cjas ◽

2010 ◽

Vol 55 (No. 1) ◽

pp. 496-504 ◽

Cited By ~ 1

Author(s):

A. Cieslak ◽

J. Kowalczyk ◽

M. Czauderna ◽

A. Potkanski ◽

M. Szumacher-Strabel

Keyword(s):

Fatty Acids ◽

Milk Fat ◽

Rapeseed Oil ◽

Unsaturated Fatty Acids ◽

Linseed Oil ◽

Fatty Acid Content ◽

Latin Square ◽

Feed Additives ◽

Control Group ◽

Experimental Conditions

The aim of our research was to evaluate whether rapeseed and linseed oils used as feed additives to ewe's diets allow to achieve the higher secretion of unsaturated fatty acids into milk with extended emphasis on the conjugated linoleic acid concentration. Two experiments were carried out on 6 lactating ewes in two independent 3 × 3 Latin square designs. In each feeding cycle the animals were subjected to one of the treatments: (1) without oil supplementation, (2) with 3.5% of rapeseed oil (RS) or linseed oil (LS), (3) with 7.0% of rapeseed oil or linseed oil. Feeding diets rich in mono- and polyunsaturated fatty acids to lactating ewes under our experimental conditions resulted in demanded changes in milk fatty acid content without causing milk fat depression or modification of other milk constituents. Milk fat was improved in unsaturated fatty acids including C18:2 c9 t11 CLA isomer without changing the milk fat concentration. The C18:2 c9 t11 concentration increased from 0.03 (FAME, %) in the control group to 0.21 and 0.12 (FAME, %) in groups receiving 3.5 and 7% RS, respectively. The diet with 7% LS also elevated the C18:2 c9 t11 level in milk from 0.04 (FAME, %) in the control group to 0.11 (FAME, %). Hence a conclusion is drawn that plant oils may be an important source of monoenic and also polyenic fatty acids in milk, however, our research and analysis of literature data indicate that results are strictly dietary and supplement-dependent.

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Influence of Dietary Fatty Acid and Î±-Tocopherol Supply on Tissue Fatty Acid Profiles, Î±-Tocopherol Content and Lipid Oxidation in Pigs

Proceedings of the British Society of Animal Science ◽

10.1017/s0308229600031147 ◽

1996 ◽

Vol 1996 ◽

pp. 147-147 ◽

Cited By ~ 1

Author(s):

G.E. Onibi ◽

J.R. Scaife ◽

V.R. Fowler ◽

I. Murray

Keyword(s):

Fatty Acids ◽

Fatty Acid ◽

Lipid Oxidation ◽

Unsaturated Fatty Acids ◽

Tocopherol Content ◽

Quality And Safety ◽

Fatty Acid Profiles ◽

Healthy Human ◽

Muscle Lipid ◽

Pig Muscle

Unsaturated fatty acids especially n-3 polyunsaturated fatty acids (PUFA) are recognised as important components of a healthy human diets and increased intake has been shown to reduce the incidence of cardiovascular diseases (BNF, 1992). These fatty acids are susceptible to oxidation and lipid oxidation in meat may adversely affect meat quality and safety. However, tissue Î±-tocopherol (AT) may reduce oxidative changes. In this study, the effect of increased dietary supply of AT and unsaturated fatty acids on tissue AT content, fatty acid profiles and oxidative stability of pig muscle lipid was assessed.

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Fatty Acid Composition and Contents of Fish of Genus Salvelinus from Natural Ecosystems and Aquaculture

Biomolecules ◽

10.3390/biom12010144 ◽

2022 ◽

Vol 12 (1) ◽

pp. 144

Author(s):

Michail I. Gladyshev ◽

Alexander A. Makhrov ◽

Ilia V. Baydarov ◽

Stanislava S. Safonova ◽

Viktor M. Golod ◽

...

Keyword(s):

Fatty Acids ◽

Muscle Tissue ◽

Nutritive Value ◽

Ecological Factors ◽

Natural Ecosystems ◽

Farmed Fish ◽

Fa Composition ◽

Label Information ◽

Wet Weight ◽

Docosahexaenoic Acids

Fatty acids (FA) of muscle tissue of Salvelinus species and its forms, S. alpinus, S. boganidae, S. drjagini, and S. fontinalis, from six Russian lakes and two aquacultures, were analyzed. Considerable variations in FA compositions and contents were found, including contents of eicosapentaenoic and docosahexaenoic acids (EPA and DHA), which are important indicators of fish nutritive value for humans. As found, contents of EPA+DHA (mg·g−1 wet weight) in muscle tissue of Salvelinus species and forms varied more than tenfold. These differences were supposed to be primarily determined by phylogenetic factors, rather than ecological factors, including food. Two species, S. boganidae and S. drjagini, had the highest EPA+DHA contents in their biomass and thereby could be recommended as promising species for aquaculture to obtain production with especially high nutritive value. Basing on revealed differences in FA composition of wild and farmed fish, levels of 15-17-BFA (branched fatty acids), 18:2NMI (non-methylene interrupted), 20:2NMI, 20:4n-3, and 22:4n-3 fatty acids were recommended for verifying trade label information of fish products on shelves, as the biomarkers to differentiate wild and farmed charr.

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