Formulation of Hair Tonic of Meniran (Phyllanthus niruri L.) Ethanol Extract as Hair Grower in Male White Rat (Rattus norvegicus) Wistar Strain

Uji Efektivitas Kombinasi Ekstrak Etanol Jamur Kayu (Ganoderma lucidum) dengan Daun Sirsak (Annona muricata) terhadap Tikus Putih Galur WIstar (Rattus norvegicus) Hiperglikemia(Effectivity Test of Wood Mushroom (Ganoderma lucidum) with Soursop Leaves (Annona muricata) Ethanol Extract Combination to Hyperglicemic Wistar Strain White Mouse (Rattus norvegicus) Jesica Christy Naibaho*, Rooije R.H. Rumende, Hanny PontororingProgram Studi Biologi FMIPA Universitas Sam RatulangiJl. Kampus Unsrat, Manado 95115*Email korespondensi: [email protected], (Article History: Received 31-12-2019; Revised 15-01-2020; Accepted 24-01-2020) Abstrak Diabetes merupakan penyakit dengan indikasi pankreas tidak dapat memproduksi hormon insulin dalam jumlah cukup ataupun ketidakmampuan tubuh mempergunakan produksi insulin dengan baik. Penelitian ini bertujuan untuk menguji efektifitas dua konsentrasi ekstrak kombinasi jamur kayu (G. lucidum) dengan daun sirsak (A. muricata) dibandingkan dengan obat diabetes jenis glibenklamid. Penelitian menggunakan metode eksperimental toksisitas akut dengan lima kelompok perlakuan dan lima ulangan. Analisis data yang digunakan adalah uji Friedman dengan uji lanjut Mann-Whitney. Hasil penelitian menunjukkan jumlah konsentrasi ideal kombinasi ekstrak jamur kayu (G. lucidum) dengan daun sirsak (A. muricata) yang dapat diinduksi terhadap tikus galur wistar hiperglikemia adalah 150 mg/kgBB dan 300 mg/kgBB tikus. Kombinasi ekstrak berpengaruh terhadap kontrol gula darah tikus galur wistar. Konsentrasi optimum kombinasi ekstrak di antara kedua dosis uji ekstrak adalah 300 mg/kgBB. Dosis 300 mg/kgBB kombinasi ekstrak dibandingkan dengan glibenklamid.Kata Kunci: Diabetes, pankreas,hormon insulin,glibenklamid Abstract Diabetes is a pancreatic disease that can not produce enough insulin hormone or body’s inability to employ insulin hormones properly.The purpose of this study was to tested the effectiveness of two concentrations of wood mushrooms (G. lucidum) with soursop leaves (A. muricata) extract compared to glibenclamide as one of diabetes remedy. Research used acute toxicity experimental methods with five treatment groups and five repetitions. Data were analyzed using Friedman’s test and Mann-Whitney’s test. The results showed that the ideal number of wood mushrooms (G. lucidum) with soursop leaves (A. muricata) extract which can induced to wistar hyperglycemic mouse were 150 mg/kgBM and 300 mg/kgBM. Extract combination has an effect on mice glucose control. Optimum concentration of Extract Combination between the two extract test doses was 300 mg/kgBM. 300 mg/kgBM dose of wood mushrooms (G. lucidum) and soursop leaves (A. muricata) extract avowed more effective than glibenclamide.Key words: Diabetes, pancreas, insulin hormone, glibenklamide

Download Full-text

UJI EFEKTIVITAS SENYAWA FLAVONOID DARI EKSTRAK ETANOL DAUN SAWILANGIT (Vernonia cinerea (L.) Less) SEBAGAI ANTIPIRETIK PADA TIKUS PUTIH JANTAN GALUR WISTAR (Rattus norvegicus) YANG DIINDUKSI VAKSIN DPT

PHARMACON ◽

10.35799/pha.9.2020.31367 ◽

2020 ◽

Vol 9 (4) ◽

pp. 572

Author(s):

Asriyani Samiun ◽

Edwin De Queljoe ◽

Irma Antasionasti

Keyword(s):

Rattus Norvegicus ◽

Ethanol Extract ◽

Male Rats ◽

Test Material ◽

Control Group ◽

White Rats ◽

Wistar Strain ◽

One Way Anova ◽

Vernonia Cinerea ◽

Dpt Vaccine

ABSTRACTSawilangit leaves (Vernonia Cinerea (L.) Less) is a plant that contains flavonoids as an antipyretic. The purpose of this study was to determine the effectiveness of flavonoid compounds from ethanol extract of sawilangit (Vernonia cinerea (L.) Less) leaves as an antipyretic in male white rats Wistar strain (Rattus norvegicus) induced by DPT vaccine. A total of 15 male rats were divided into 5 treatment groups, namely the negative control group (CMC 1%), the positive control group (paracetamol), and the ethanol extract group of sawilangit leaves with a dose of 200 mg, a dose of 400 mg, a dose of 800 mg. Each mouse was induced intramuscularly with a DPT vaccine at a dose of 0.3 ml. Temperature measurements were carried out before and after induction, then every 30 minutes after giving the test material from the 30th minute to the 180th minute. Data were analyzed statistically with One Way ANOVA and continued with LSD test to see significant differences between treatments. The results showed that the ethanol extract of sawilangit leaves had an antipyretic effect on male white rats Wistar strain..Key words: Antipyretics, Vernonia Cinerea, male white rats ABSTRAKDaun Sawilangit (Vernonia Cinerea (L.) Less) adalah salah satu tanaman yang mengandung zat Flavonoid sebagai antipiratik. Tujuan penelitian ini untuk mengetahui adanya efektivitas senyawa flavonoid dari ekstrak etanol daun sawilangit (Vernonia cinerea (L.) Less) sebagai antipiretik pada tikus putih jantan galur wistar (Rattus norvegicus) yang diinduksi vaksin DPT. Sebanyak 15 ekor tikus putih jantan dibagi 5 kelompok perlakuan yaitu kelompok kontrol negatif (CMC 1%), kelompok kontrol positif (parasetamol), dan kelompok ekstrak etanol daun sawilangit dosis 3,6 mg, dosis 7,2 mg, dosis 14,4 mg. setiap tikus diinduksi vaksin DPT dosis 0,3 ml secara intramuscular. Pengukuran suhu dilakuan sebelum dan sesudah induksi, kemudian setiap 30 menit setelah pemberian bahan uji dari menit ke-30 sampai menit ke-180. Data dianalisis secara statistika dengan One Way ANOVA dan dilanjutkan uji LSD untuk melihat beda nyata antar perlakuan. Hasil penelitian menunjukan bahwa Ekstrak etanol daun sawilangit mempunyai efek antipiretik terhadap tikus putih jantan galur wistar. Kata kunci: Antipiretik, Vernonia Cinerea, tikus putih jantan

Download Full-text

Lotion Formulation of Ethanolic Extract of Meniran (Phyllanthus niruri L.) As Hair Growth Promoter in Male White Rats (Rattus norvegicus) of Wistar Strain

Pharmaceutical Sciences and Research ◽

10.7454/psr.v6i1.4266 ◽

2019 ◽

Vol 6 (1) ◽

Author(s):

Sri Luliana ◽

◽

Rise Desnita ◽

Sehro Sehro ◽

◽

...

Keyword(s):

Rattus Norvegicus ◽

Hair Growth ◽

Ethanolic Extract ◽

Growth Promoter ◽

Phyllanthus Niruri ◽

White Rats ◽

Wistar Strain

Download Full-text

Uji Efektivitas Kombinasi Ekstrak Etanol Jamur Kayu (Ganoderma lucidum) dengan Daun Sirsak (Annona muricata) terhadap Tikus Putih Galur WIstar (Rattus norvegicus) Hiperglikemia

JURNAL BIOS LOGOS ◽

10.35799/jbl.10.1.2020.27979 ◽

2020 ◽

Vol 10 (1) ◽

pp. 13

Author(s):

Jesica Christy Naibaho ◽

Rooije R.H. Rumende ◽

Hanny Pontororing

Keyword(s):

Rattus Norvegicus ◽

Ganoderma Lucidum ◽

Ethanol Extract ◽

Pancreatic Disease ◽

White Mouse ◽

Annona Muricata ◽

Treatment Groups ◽

Wistar Strain ◽

Friedman's Test ◽

Ideal Number

Uji Efektivitas Kombinasi Ekstrak Etanol Jamur Kayu (Ganoderma lucidum) dengan Daun Sirsak (Annona muricata) terhadap Tikus Putih Galur WIstar (Rattus norvegicus) Hiperglikemia(Effectivity Test of Wood Mushroom (Ganoderma lucidum) with Soursop Leaves (Annona muricata) Ethanol Extract Combination to Hyperglicemic Wistar Strain White Mouse (Rattus norvegicus) Jesica Christy Naibaho*, Rooije R.H. Rumende, Hanny PontororingProgram Studi Biologi FMIPA Universitas Sam RatulangiJl. Kampus Unsrat, Manado 95115*Email korespondensi: [email protected], (Article History: Received 31-12-2019; Revised 15-01-2020; Accepted 24-01-2020) Abstrak Diabetes merupakan penyakit dengan indikasi pankreas tidak dapat memproduksi hormon insulin dalam jumlah cukup ataupun ketidakmampuan tubuh mempergunakan produksi insulin dengan baik. Penelitian ini bertujuan untuk menguji efektifitas dua konsentrasi ekstrak kombinasi jamur kayu (G. lucidum) dengan daun sirsak (A. muricata) dibandingkan dengan obat diabetes jenis glibenklamid. Penelitian menggunakan metode eksperimental toksisitas akut dengan lima kelompok perlakuan dan lima ulangan. Analisis data yang digunakan adalah uji Friedman dengan uji lanjut Mann-Whitney. Hasil penelitian menunjukkan jumlah konsentrasi ideal kombinasi ekstrak jamur kayu (G. lucidum) dengan daun sirsak (A. muricata) yang dapat diinduksi terhadap tikus galur wistar hiperglikemia adalah 150 mg/kgBB dan 300 mg/kgBB tikus. Kombinasi ekstrak berpengaruh terhadap kontrol gula darah tikus galur wistar. Konsentrasi optimum kombinasi ekstrak di antara kedua dosis uji ekstrak adalah 300 mg/kgBB. Dosis 300 mg/kgBB kombinasi ekstrak dibandingkan dengan glibenklamid.Kata Kunci: Diabetes, pankreas, hormon insulin, glibenklamid Abstract Diabetes is a pancreatic disease that can not produce enough insulin hormone or body’s inability to employ insulin hormones properly.The purpose of this study was to tested the effectiveness of two concentrations of wood mushrooms (G. lucidum) with soursop leaves (A. muricata) extract compared to glibenclamide as one of diabetes remedy. Research used acute toxicity experimental methods with five treatment groups and five repetitions. Data were analyzed using Friedman’s test and Mann-Whitney’s test. The results showed that the ideal number of wood mushrooms (G. lucidum) with soursop leaves (A. muricata) extract which can induced to wistar hyperglycemic mouse were 150 mg/kgBM and 300 mg/kgBM. Extract combination has an effect on mice glucose control. Optimum concentration of Extract Combination between the two extract test doses was 300 mg/kgBM. 300 mg/kgBM dose of wood mushrooms (G. lucidum) and soursop leaves (A. muricata) extract avowed more effective than glibenclamide.Key words: Diabetes, pancreas, insulin hormone, glibenklamide

Download Full-text

Effects of Sambiloto Ethanol Extract on Fatty Liver, SGOT/SGPT Levels and Lipid Profile of Wistar Strain White Rat (Rattus norvegicus) Exposed to High-Fat Diet

Folia Medica Indonesiana ◽

10.20473/fmi.v54i2.8856 ◽

2018 ◽

Vol 54 (2) ◽

pp. 89

Author(s):

FX Himawan Haryanto Jong ◽

Ari Gunawan ◽

Mochamad Wirono Aman Santoso ◽

Susilowati Anjani ◽

Ni Wayan Tirthaningsih ◽

...

Keyword(s):

Fatty Liver ◽

Lipid Profile ◽

Rattus Norvegicus ◽

High Fat Diet ◽

Density Lipoprotein ◽

Ethanol Extract ◽

Homogeneity Test ◽

High Fat ◽

White Rats ◽

Wistar Strain

The aim of this study was to analyze the effects of ethanol extract of sambiloto (Andrographis paniculata) on fatty liver percentage, serum glutamic oxaloacetic transaminase (SGOT) level and lipid profile of wistar strain white rat exposed to high fat diet. The study used randomized post test only control group design. Total sample was 50 male wistar strain rats (Rattus norvegicus) divided randomly into 5 groups (randomization). The normality test used was Kolmogorov Smirnov test (a=0.05). The homogeneity test used was Levene test (a=0.05). The comparative test was done using Anova test (analysis of variance) (a=0.05) or Brown-Forsythe test (a=0.05). The correlation test was done using Pearson test (a=0.05). The administration of sambiloto ethanol extract with doses of 100, 200 and 400 milligrams (mg)/kilogram (kg) body weight (BW) decreased the percentage of fatty liver (r=-0.950), SGOT (r=-0.964)/SGPT (r=(R=-0.973)/LDL (low-density lipoprotein) (r=-0.960) and increased HDL (high-density lipoprotein) levels (r=-0.923)=0.956) in white rats exposed to a high-fat diet. In conclusion, increased dose of ethanol extract of sambiloto can decrease the percentage of fatty liver, SGOT/SGPT and total cholesterol/TG/LDL and increase HDL level of white rats exposed to high fat diet.

Download Full-text

The Hepatotoxic and Nephrotoxic Effects of Purwoceng (Pimpinella pruatjan Molk.) Roots Ethanol Extract Administration in Subchronic Dose

Molekul ◽

10.20884/1.jm.2021.16.2.729 ◽

2021 ◽

Vol 16 (2) ◽

pp. 163

Author(s):

Fitranto Arjadi ◽

Nur Signa Aini Gumilas ◽

Ika Murti Harini ◽

Vitasari Indriani ◽

Lantip Rujito

Keyword(s):

Dose Level ◽

Rattus Norvegicus ◽

Ethanol Extract ◽

Control Group ◽

Serum Glutamic Pyruvic Transaminase ◽

Control Group Design ◽

Wistar Strain ◽

Post Test ◽

Group A ◽

Group B

Purwoceng (Pimpinella pruatjan Molk) is an original Indonesian herb which is known to have an aphrodisiac effect. The active compounds in Purwoceng potentially have hepatotoxic and nephrotoxic effects.This study was aimed to analyze the effect of subchronic administration of Purwoceng roots ethanol extract to Wistar Strain of Rattus norvegicus rats. The method of this subchronic toxicity study was an experimental post test only with control group design. Forty male Rattus norvegicus were randomly divided into four groups and get 28-days treatment. Group A as control received aquadest and 1% CMC (carboxymethyl cellulose), group B, C, and D were given Purwoceng roots ethanol extract of 42, 84, and 168 mg/KgBW/day and 1% CMC. Parameters tested were the levels of urea, creatinine, SGPT (serum glutamic pyruvic transaminase), SGOT(serum oxaloacetic pyruvic transaminase), kidney and hepar histopatology.The results showed a statistically significant for the liver histopathological in group B, creatinine, urea, and kidney histopathology in group C. Our study concluded that subchronic administration of Purwoceng (Pimpinella pruatjan Molk) roots ethanol extract could induce hepatotoxicity at the 42 mg/KgBW/day dose level and nephrotoxicity at the 84 mg/KgBW/day dose level.

Download Full-text

Effectiveness of Sargassum sp. ethanolic extract on traumatic ulcers healing in the labial mucosa of Wistar strain (Rattus norvegicus)

Padjadjaran Journal of Dentistry ◽

10.24198/pjd.vol31no1.16513 ◽

2019 ◽

Vol 31 (1) ◽

pp. 73

Author(s):

Annisya Ristie Arwidasari ◽

Twi Agnita Cevanti ◽

Isidora Karsini Soewondo

Keyword(s):

Wound Healing ◽

Rattus Norvegicus ◽

Economic Value ◽

Methyl Cellulose ◽

Ethanolic Extract ◽

Ethanol Extract ◽

Test Results ◽

Wistar Strain ◽

Acid Gel ◽

Iron And Calcium

Introduction: Oral ulcer lesions are one of the most common lesions in the oral cavity. The prevalence of traumatic ulcers is quite high compared to other oral lesions. Brown algae, Sargassum sp. is one of the algae contains flavonoids, vitamin A, vitamin C, iron, and calcium, which play a role in wound healing and has economic value. This study was aimed to determine the effectiveness of Sargassum sp. ethanolic extract on traumatic ulcers healing in the labial mucosa of Wistar strain (Rattus norvegicus). Methods: 28 Wistar strain (Rattus norvegicus) weighed 150-200 grams. The samples were divided into 4 groups (K1, K2, P1, P2); each group consisted of 7 mice. K1 group was administered with Hydroxy Propyl Methyl Cellulose (HPMC), K2 was administered with 0.2% hyaluronic acid gel, P1 was administered with 50% Sargassum sp. extract gel, and P2 was administered with 75% Sargassum sp. extract gel. In each group, the ulcer was made on the mice labial mucosa; then the ulcer diameter was measured and observed on day 1 and day 7. Results: The mean diameter ± standard deviation of traumatic ulcer calculations were K1 (1.09 ± 0.40), K2 (1.81 ± 0.67), P1 (1.39 ± 0.43) and P2 (2.16 ± 0.49) respectively. One way ANOVA test results showed significant differences in K1, K2, P1, and P2 groups. Conclusion: Ethanol extract of Sargassum sp. is effective in accelerating the traumatic ulcers healing at the concentration of 75%.Keywords: Traumatic ulcer, Sargassum sp., wound healing.

Download Full-text

UJI EFEK ANALGESIK EKSTRAK ETANOL DAUN KELOR (Moringa oleifera Lam.) PADA TIKUS PUTIH JANTAN GALUR WISTAR (Rattus norvegicus)

PHARMACON ◽

10.35799/pha.9.2020.30015 ◽

2020 ◽

Vol 9 (3) ◽

pp. 325

Author(s):

Alyah A.P Tamimi ◽

Edwin De Queljoe ◽

Jainer P. Siampa

Keyword(s):

Rattus Norvegicus ◽

Analgesic Effect ◽

Moringa Oleifera ◽

Ethanol Extract ◽

Negative Control ◽

Test Material ◽

Control Group ◽

White Rats ◽

Wistar Strain ◽

Moringa Oleifera Lam

ABSTRACTAnalgesic medicinals are medicinals that can inhibit pain. Moringa leaves (Moringa oleifera Lam.) are known to contains alkaloid and flavonoid which have analgesic effects.The purpose of this study was to determine the analgesic effects of the ethanol extract of Moringa leaves on male white rats wistar strain (Rattus norvegicus). A total of 15 rats were divided into 5 treatment groups, namely the negative control group (CMC), positive control group (paracetamol), and the group of ethanol extract of Moringa leaves with different doses of 0.1 g, 0.2 g, and 0.4 g. Rat response was observed before giving test material and after giving test material from the 30th, 60th, 90th, and 120th minutes. Data were analyzed using ANOVA then continued with LSD test. The results showed that the ethanol extract of Moringa leaves with dosages of 0.1 g, 0.2 g, and 0.4 g had an analgesic effect on male white rats wistar strain. And among these three doses the best in providing analgesic effect is a dose of 0.4 g. Keywords: Analgesic, Moringa leaf (Moringa oleifera Lam.), Rattus norvegicus. ABSTRAKObat analgesik merupakan obat yang dapat menghambat rasa nyeri. Daun kelor (Moringa oleifera Lam.) diketahui mengandung alkaloid dan flavonoid yang memiliki efek analgesik. Tujuan penelitian ini untuk mengetahui adanya efek analgesik dari ekstrak etanol daun kelor (Moringa oleifera Lam.) pada tikus putih jantan galur wistar (Rattus norvegicus). Sebanyak 15 ekor tikus dibagi 5 kelompok perlakuan, yaitu kelompok kontrol negatif, kelompok kontrol positif, dan kelompok ekstrak etanol daun kelor dengan dosis berbeda 0,1 g, 0,2 g, dan 0,4 g. Respon tikus diamati sebelum pemberian bahan uji dan setelah pemberian bahan uji dari menit ke 30, 60, 90, dan 120. Data dianalisis menggunakan ANOVA kemudian dilanjutkan uji LSD. Hasil penelitian menunjukkan bahwa ekstrak etanol daun kelor dengan dosis 0,1 g 0,2 g, dan 0,4 g memiliki efek analgesik terhadap tikus putih jantan galur wistar. Dan diantara ketiga pemberian dosis tersebut yang paling baik dalam memberikan efek analgesik yaitu dosis 0,4 g. Kata kunci: Analgesik, Daun Kelor (Moringa oleifera Lam.), Rattus norvegicus.

Download Full-text