scholarly journals Uji Efek Analgetik Infusa Daun Beluntas (Pluchea indica L.) Pada Mencit Jantan Galur Swiss

2018 ◽  
Vol 7 (2) ◽  
pp. 61-67
Author(s):  
Sri S. Wahyuningsih ◽  
Linda Widyastuti
Keyword(s):  
Body Weight ◽  
Acetic Acid ◽  
Positive Control ◽  
Test Substance ◽  
Male Mice ◽  
Group Iii ◽  
Group I ◽  
Homogeneity Of Variance ◽  
Kolmogorov Smirnov ◽  
Swiss Strain

Beluntas leaves (Pluchea indica L.) have been known as analgetic reducer. The study about this research has been going on right now. The goal of this research is to study the analgesic effect of beluntas (P. indica) leaf infused into male mice of swiss strain. The method used is the stretching chemical stimuli using acetic acid as an inducer of pain. Healthy male mice of Swiss strain were divided into five groups, and each group consisted of 5 mice. Group I was given paracetamol at a dose of 65 mg/kg of body weight, group II were given distilled water , the group III-V were given beluntas leaf infuse in the variation of 10 %, 20 % and 40 %. Thirty minutes after test substance application, acetic acid of 100 mg/kg of body weight were given intraperitoneally in all groups and stretching of mice was observed every 5 minutes for 1 hour. The data was analyzed by using normality test of Kolmogorov-Smirnov; the  test of homogeneity of variance was analyzed by ANOVA and post hoc tests were to differenciate the percentage analgetic of every group. Statistical test showed normal distributed and homogeneous data; there are significant differences of percentage analgesic between paracetamol and beluntas leaf infuse of 10, 20, and 40 % (p < 0.05). There was significant differences between infuse of beluntas leaves with positive control (parasetamol) in mice. Key words: Analgetic, leave of beluntas (P. indica), infuse, mice.

scholarly journals UJI EFEK ANALGETIKA EKSTRAK BUAH KAKTUS (Opuntia elatior Mill.) PADA MENCIT (Mus musculus) YANG DIINDUKSI ASAM ASETAT

2015 ◽  
Vol 1 (1) ◽  
pp. 16-21
Author(s):  
Gissa Amellia ◽  
Ihwan Ihwan ◽  
Akhmad Khumaidi
Keyword(s):  
Body Weight ◽  
Acetic Acid ◽  
Effective Dose ◽  
Mus Musculus ◽  
Positive Control ◽  
Negative Control ◽  
Group Iv ◽  
Group V ◽  
Group Iii ◽  
Group I

Pain is a mechanism of body defense. One way to allay or decreasing the pain is using medicines that have analgetic effect. The aim of this study is determine the analgetic effect of cactus fruits (Opuntia elatior Mill.) extract and the most effective dose to giving analgetic effect. This study used writhing method by the injection acetic acid 1% as induced. Group I as a negative control used natrium carboxy methylcellulose 1%, group II as a positive control used aspirin suspension which dosage was 1,3 mg/20g BB, group III as a extract of cactus fruits give to dose 50 mg/kg BB, group IV dose 100 mg/kg BB, and group V dose 150 mg/kg BB. The result shows that cactus fruits extract to giving analgetic effect judging from the reduction strength writhing reflex to protection percentage at dose 50 mg/kg BB (11,26%), 100 mg/kg BB (42,10%), 150 mg/kg BB(60,14%) and the positif control (75,34%), at the positif control shows that higher protection percentage. Effectiveness percentage analgetic extract of cactus fruits at dose of 50 mg/kg BB (14,4%), 100 mg/kg BB (55,36%), 150 mg/kg BB (79,95%) and positif control (100%). The data which is got from the calculation later is analyzed by Kruskal Wallis. The result showed that at dose of 150 mg/kg BB is p: 0,016 (p<0,05) when compared with the positive control. So that is different significantly between dose 150 mg/kg BB and the positive control. The extract showed a optimal analgetic effect characterized by a significant reduction in the number of writhes or abdominal stretches in mice with dose 150 mg/kg BB body weight used when compared to the cactus fruits extract dose 50 and 100 mg/kg BB

scholarly journals Application of Scaevola taccada (Gaertn.) Roxb. Reduce Pro-inflammatory Cytokines Interleukin-1β in Sprague Dawley Mice Suffering from Mastitis

2020 ◽  
Vol 8 (A) ◽  
pp. 423-427
Author(s):  
Prihantono Prihantono ◽  
Salman Ardi Syamsu ◽  
Nilam Smaradhania ◽  
Mardiana Ahmad ◽  
Nurul Aini Siagian ◽  
...  
Keyword(s):  
Body Weight ◽  
Positive Control ◽  
Mean Value ◽  
Negative Control ◽  
Interleukin 1Β ◽  
Sprague Dawley ◽  
Leaf Extracts ◽  
Group Iii ◽  
Group I ◽  
The Mean

BACKGROUND: Mastitis is an inflammation of the breast tissue, usually caused by bacteria. Mastitis stimulates pro-inflammatory cytokines. The cytokine interleukin-1β (IL-1β) is a crucial mediator of the inflammatory response. This cytokine has adverse effects of hosting immunity that mediates resistance to pathogens and also exacerbates damage during chronic disease and acute tissue injury. Scaevola taccada (Gaertn.) Roxb. has been used as an ethnomedicine for healing sores in several provinces in Indonesia. AIM: This study aimed to assess the profile of pro-inflammatory cytokine IL-1β through the treatment effect of leaf extracts of S. taccada (Gaertn.) Roxb. as adjuvant for healing mastitis. METHODS: This study was a true control group experiment using the pre-test-post-test control design aimed to measure the effect of hydroalcoholic compounds in leaf extracts of S. taccada on the systemic pro-inflammatory activity of interleukin-1β (IL-1β). The treated animals were 18 mice of Sprague Dawley strain induced by Staphylococcus aureus. These treated mice were divided into three groups in which each group consisted of six mice. The mice in the Group I (negative control) were given 1 ml aquabides/250 g body weight, those in the Group II (positive control) were delivered with 9.6 mg/ml amoxicillin/250 g body weight, and those in the Group III (experimental) were given 9.6 mg amoxicillin/250 g body weight + 400 mg/ml leaf extracts of S. taccada/g body weight for 5 days, respectively. Pathological examinations were carried out from the inflamed tissues to prove the healing process of the treated mice. IL-1Β levels were determined using the enzyme-linked immunosorbent assay method. Data were analyzed using ANOVA and post hoc tests. RESULTS: There were statistically significant differences of IL-1β levels after the administration of leaf extracts of S. taccada among all the treated mice groups at p < 0.05. The Group III had the lowest IL-1β level with the mean value ± 1.45 pg/ml compared to the IL-1β level in the Group II (positive control) with the mean value ± 3.82 pg/ml and the IL-1β level in the Group I (negative control) with the with mean value ± 5.22 pg/ml. The pathological analysis of breast tissues of the treated mice proved that leaf extracts of S. taccada (Gaertn Roxb.) could reduce damaged tissues, cellular infiltration, and subcutaneous edema induced by this pathogenic microorganism. CONCLUSION: Leaf extracts of S. taccada had a significant function as adjuvant for healing mastitis by reducing pro-inflammatory cytokine IL-1β.

scholarly journals PENGARUH PEMBERIAN EKSTRAK AKAR PASAK BUMI (EURYCOMA LONGIFORA JACK) TERSTANDAR TERHADAP GAMBARAN HISTOPATOLOGIK TESTIS TIKUS WISTAR

2017 ◽  
Vol 13 (3) ◽  
Author(s):  
Condro Suro Miyarso ◽  
Tri Cahyani Widiastuti ◽  
Naelaz Zukhruf Wakhidatul Kiromah
Keyword(s):  
Body Weight ◽  
Testosterone Level ◽  
Positive Control ◽  
Negative Control ◽  
Male Rats ◽  
Histopathological Changes ◽  
Group Iii ◽  
Eurycoma Longifolia ◽  
Group I ◽  
Eurycoma Longifolia Jack

The root of pasak bumi (Eurycoma longifolia, Jack) is one of plant from Indonesia known as aphrodisiac. The study about its standardized extract as aphrodisiac was show increasing libido, testosterone level, FSH and LH on male rats. This study was conducted to evaluate testosterone level and histopathological changes of the testes of male rats of standardized extract of pasak bumi root. There are 50 old male rats which ages 3-4 months old divided to five groups. Group I as negative control was administered aquadestilata. Group II as positive control was administered testosterone (Andriol®). Group III, IV and V ware given standardized extract of pasak bumi root at the dose 50, 100 and 200 mg/kg body weight Respectively. The extract was given orally twice a day for six days and forty nine days and then the testes was taken out on 7th and 50th day. The histopathological of the testes was evaluated using quantitative parameter by the presence of testes weight, number and diameter of Leydig cells. Testosterone level was taken on 7th and 50th day used ELISA method. The results were analyzed using parametric test, one way ANOVA (P<0,05), post hoc Dunnet (2-sided) (P < 0,05). The result of this study shows that the standardized extract of pasak bumi root was not effect on histopathological changes of the testes but it could increased the level of testosterone m at the dose 100 and 200 mg/kg body weight.  Key word : Eurycoma longifolia, Jack, aphrodisiac, histopathological testes testosteron

scholarly journals Pengembangan Produk Minuman Tonikum Kulit Kayu Akway (Drymis piperita): Prospek Penambah Stamina Tubuh Secara In Vivo

2021 ◽  
Vol 13 (1) ◽  
pp. 44-51
Author(s):  
Noviana Hallik ◽  
Rani D. Pratiwi ◽  
Elsye Gunawan
Keyword(s):  
Body Weight ◽  
Positive Control ◽  
Total Period ◽  
Physical Quality ◽  
Group Iii ◽  
Group I ◽  
The One ◽  
Base Group

Akway bark (Drymis piperita) from Papua is widely used by local people in Arfak Mountains of West Papua to increase stamina. The purpose of this study was to determine the physical quality and tonic effects of Akway bark (bark tonic drink D. piperita) on mice (Mus musculus). The swimming resistance used to determine the tonic effect of mice (24 individuals) which divided into 6 groups. Group I was given water, group II tonic drink base, group III as positive control of 50 mg caffeine energy drinks, groups IV, V, and VI were given bark tonic drink 10mg /kg body weight, 30 mg/kg body weight, and 50mg /kg body weight respectively. The total period swimming until fatigue was measured and used as an index of swimming capacity. The results of  physical quality of the Akway bark tonic drink has a sweet and sour taste, with clear dark brown color, had distinctive aroma with pH of 4, and its volume was 100 ml with solubility deposits on day 21. The results of pre-clinical testing shown the highest dose was 50 mg/ weight swimming time 9.28 minutes and having better stamina. The next was with dose of 30 mg/kgBW, swimming for for 6.01 minutes and the last was dose of 10 mg /kgBW swimming for 3.23 minutes. The results of the one-way ANOVA test showed that it was significantly different between the 10 mg/kgBW, 30 mg/kgBW and 50 mg/kgBW groups. Key words: drink; D. piperita tonic; effect; mice; Papua. 

scholarly journals The Influence On Bodyweight In Male Mice (Mus Musculus) Induced By Monosodium Glutamate (Msg)

2018 ◽  
Vol 1 (1) ◽  
pp. 01-03
Author(s):  
Yunita Sari Pane
Keyword(s):  
Body Weight ◽  
Monosodium Glutamate ◽  
Group Iv ◽  
Post Treatment ◽  
The Body ◽  
Male Mice ◽  
Group Iii ◽  
Group I ◽  
Significant Difference ◽  
Group Ii

The used of monosodium glutamate (MSG) oftenly found in almost every food that we consumed everyday. It suggested that MSG can increase appetite, can caused intake much foods, and increased the bodyweight. The aim of study is to prove the changes that occur in male mice by induce of MSG. The experimental study used 24 male mice, 30-45 gram, 12-14 weeks divided into 4 groups, ie group-I (negative control/placebo given aquadest 0.2 cc/20gBW mice); group-II, MSG with the dose of 5 mg/gBW mice); group-III, MSG with the dose of 10 mg/gBW mice and group-IV, MSG with the dose of 20 mg/gBW mice. All of these treatments were administered orally for 14 days. Permanent cervical fracture execution was performed at the end of the study. The body weight measured in pre and post treatment. The significant difference of data between different groups was compared by ANOVA followed by Tukey test. The compared of between pre –post treatment used paired test to analyze the changes of bodyweight This study proved that the comparison of mean values ± SEM of body weight between groups were not significantly different; group I (39,83±3,26), group II (39,33±2,04), group III (38,00±1,39) and group IV (38,33±1,76) (p = 0.930) and the changes of bodyweight pre and post treatmen also not significantly different, whereas p > 0.05. The study concluded that no changes in the body weight between groups and pasca induced by MSG. In the future studies, we consider to examining the effects MSG with longer of duration and more variations doses in MSG.

scholarly journals EVALUATION OF ANTI-DIARRHOEAL EFFECT OF CURCUMIN

2019 ◽  
pp. 1-2
Author(s):  
Anil Kumar
Keyword(s):  
Body Weight ◽  
Castor Oil ◽  
Positive Control ◽  
Albino Rats ◽  
Dependent Manner ◽  
Standard Drug ◽  
Group Iii ◽  
Hepatic Diseases ◽  
Group I ◽  
Diarrheal Episode

Turmeric has been used in traditional medicine as household remedy for various diseases including biliary diseases, cough, hepatic diseases, wound healing and diarrhea etc. Curcumin is the active salt present in turmeric. This study was carried out to provide scientic basis for the use of curcumin in diarrhea and to compare it with standard medicine i.e. Loperamide. The albino rats were divided into ve groups of six animals in each group. Group I (control) received vehicular uid. Group II (positive control) received standard drug for diarrhea i.e. loperamide at the dose of 2mg/kg body weight. Group III, IVand Vwere administered curcumin intragastrically by the naso-gastric tube, in the dose of 500mg/kg, 750mg/kg and 1 gm/kg body weight respectively, suspended in normal saline. After one hour of above treatment, 1 ml of castor oil was given intragastrically to all the overnight-fasted animals to induce diarrhea. Each animal was housed separately and observed for time of onset and number of diarrheal episode for 4 hours. The data was analyzed by using SPSS 11. Remarkable anti-diarrheal effect of curcumin against castor oil induced diarrhea was observed in dose dependent manner. From the present study, we can draw a conclusion that, Curcumin have signicant anti-diarrheal effect. It may be used as an adjuvant for the treatment of diarrhea, and irritable bowel syndrome.

scholarly journals UJI EFEK TONIKUM EKSTRAK ETANOL DAUN SAMBILOTO (Andrographis paniculata, Nees.) TERHADAP MENCIT JANTAN (Mus musculus L.) GALUR SWISS

1970 ◽  
Vol 6 (02) ◽  
pp. 17-22 ◽  
Author(s):  
Susi Endrawati ◽  
Feni Indriyani
Keyword(s):  
Mus Musculus ◽  
Ethanol Extract ◽  
Positive Control ◽  
Negative Control ◽  
Probit Analysis ◽  
Control Group ◽  
Test Substance ◽  
Male Mice ◽  
Group I ◽  
Body Strength

The tonic is a substance that can improve our body strength. It can recover the staff our body shortly. It can also make our body stronger and can stimulates our appetite. Knowing the most effective tonic effect dose variation among ethanol extract of bitter leaf on male mice (Mus musculus L.) Swiss Strain. This study is an experimental research design with True experimental design approach Posttest Pretest with control group. The ethanol extract of bitter leaf is made with bitter leaf quote using ethanol 96% with maceration method. The provision of treatment in test animals distinguished on several variations of dosage. In preparation extracts there are 5 groups, namely: group I, II, III, IV, V treated positive control caffeine 13 mg / kg, cooking oil as a negative control, the ethanol extract at a dose of 50 mg / kg, 100 mg / kg, 200 mg / kg. Data obtained tonic effect of added time the mice's ability to defend itself when direnangkan. Data were analyzed by ANOVA using SPSS 18.0 for windows followed by a test post hoc test and ED50 probit analysis to determine the most effective dose as a tonic. Ethanol extract of bitter leaf at a dose of 50 mg / kg have a tonic effect of 9.2 minutes, a dose of 100 mg / kg have a tonic effect of 13.4 minutes, and a dose of 200 mg / kg have a tonic effect 23 minutes. Results yield of ethanol extract of bitter leaf maceration 7.8% b/b. The ethanol extract of bitter leaf tested in test animals male mice (Mus muculus L.) at all doses provide a tonic effect, and the effect will increase along with increasing doses of test substance preparation.

Ultrastructural Lesions Produced by Alcohol and Sucrose in the Heart and Liver of C57BL Mice

1970 ◽  
Vol 28 ◽  
pp. 208-209
Author(s):  
K.K. SEKHRI ◽  
C.S. ALEXANDER ◽  
H.T. NAGASAWA
Keyword(s):  
Osmium Tetroxide ◽  
Male Mice ◽  
Alcohol Group ◽  
Group Iii ◽  
Group I ◽  
C57bl Mice ◽  
Group Ii

C57BL male mice (Jackson Lab., Bar Harbor, Maine) weighing about 18 gms were randomly divided into three groups: group I was fed sweetened liquid alcohol diet (modified Schenkl) in which 36% of the calories were derived from alcohol; group II was maintained on a similar diet but alcohol was isocalorically substituted by sucrose; group III was fed regular mouse chow ad lib for five months. Liver and heart tissues were fixed in 2.5% cacodylate buffered glutaraldehyde, post-fixed in 2% osmium tetroxide and embedded in Epon-araldite.

Seasonal variations in wool growth and heat tolerance of sheep. I. Wool growth

1960 ◽  
Vol 11 (1) ◽  
pp. 75 ◽  
Author(s):  
M Wodzicka
Keyword(s):  
Body Weight ◽  
Day Treatment ◽  
The Other ◽  
Seasonal Rhythm ◽  
Group Iii ◽  
Wool Growth ◽  
Group I ◽  
The Mean ◽  
Group Ii ◽  
The Difference

The monthly wool growth of three groups of rams was studied at Beltsville, Maryland. Group I received natural daylight (at 38° 53' N.) and was shorn monthly. Group II had a 7:17 hours of daylight to hours of darkness rhythm and was shorn every 6 months, once in winter and once in summer. Group III received natural daylight and was likewise shorn every 6 months. The rams of all groups produced more wool in summer than in winter. This difference was significant (P<0.001). The mean body weight and food intake were both greater in the winter months, which indicated that the seasonal rhythm of wool growth was not a consequence of poorer feeding in winter. The rams which were shorn monthly (group I) grew considerably more wool than the other two groups, but the difference was not statistically significant. The short-day treatment of group II did not increase the annual wool production nor decrease the seasonal rhythm of wool growth. The balance of evidence from this and other experiments indicates that temperature rather than light controls the seasonal rhythm of wool growth.

Suppression of Mesangial-Cell Proliferation by Trapidil in Glomerulonephritis Induced by Anti-Thymocyte Serum in Rats

1995 ◽  
Vol 23 (6) ◽  
pp. 458-466 ◽  
Author(s):  
M S Razzaque ◽  
M Cheng ◽  
T Taguchi
Keyword(s):  
Cell Proliferation ◽  
Body Weight ◽  
Single Dose ◽  
Growth Factor ◽  
Mesangial Cell ◽  
Platelet Derived Growth Factor ◽  
Group Iii ◽  
Group I ◽  
Mesangial Cell Proliferation ◽  
Group Ii

Trapadil (Mochida Pharmaceuticals, Japan), an antiplatelet drug, suppresses the growth of several cell types and is thought to antagonize platelet-derived growth factor. The effects of trapidil on mesangial-cell proliferation in glomerulonephritis induced by anti-thymocyte serum in Wistar rats were investigated. Control rats were treated with phosphate-buffered saline (group I); group II rats were injected with a single dose of anti-thymocyte serum (8 ml/kg body weight), and group III rats were treated with both a single dose of anti-thymocyte serum (8 ml/kg body weight) and with trapidil (5 mg/kg body weight/day). Three rats in each group were killed on day 3, and the other three on day 10. Control rats showed no significant histological changes on day 3 or day 10. In group II, on day 3, there was a marked decrease in glomerular cell numbers, with mesangiolysis. Histologically severe mesangial-cell proliferation with expansion of mesangial areas was noted on day 10. None of the rats in group III showed mesangial alterations, histologically, indicating that mesangial-cell proliferation was suppressed by trapidil. This suppression may result from antagonism of the binding of platelet derived growth factor to the specific surface receptors in the mesangial cells. Trapidil may have clinical value in the treatment of mesangial-cell proliferative glomerular diseases.

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