scholarly journals The Effectiveness of Wild Honey In Inhibiting The Growth of Bacterium on The Positive Widal Blood Culture of Enterobacteriaceae Familia

2019 ◽  
Vol 1 (2) ◽  
pp. 91-97
Author(s):  
Sri Darmawati ◽  
Eko Naning Sofyanita ◽  
Sri Sinto Dewi
Keyword(s):  
Blood Culture ◽  
Agar Plate ◽  
Enterobacter Cloacae ◽  
Inhibition Zone ◽  
Salmonella Typhi ◽  
Klebsiella Pneumonia ◽  
Medical Field ◽  
Bacteria Growth ◽  
Esherichia Coli ◽  
Inhibition Potency

Honey is a product produced by bees which is believed has many benefits in the medical field. Wild honeys are more natural than livestock honey, and it has high antimicroba activities, but the effect of the antimicroba towards the bacteria on the positive widal blood culture of Enterobacteriaceae familia member was not clear yet. This research was to find out The effectiveness of wild honey in inhibiting the growth of bacteria on the positive widal blood culture of Enterobacteriaceae familia member. Method in this research was an experimental research which was using combination of diffusion and draw well method using Klebsiella pneumonia, Esherichia coli, Salmonella typhi, Serratia marcescens, and Enterobacter cloacae bacteria sample. Wild honey as a test solution with a 25%, 40%, 55%, 70%, 85% and 100% concentrate. The result is that wild honey is effective to inhibit the growth of the Enterobacteriaceae bacteria familia, the inhibition zone which is performed on the 70% Nutrien Agar Plate (NAP) concentrate medium is a minimal concentrate that sensitive to all bacteria with the  inhibition zone similar to Kloramfinekol antibiotics. S. typhi is sensitive to all wild honey concentrate within 26,5mm, 29mm, 31,5mm, 32mm, 35mm, and 38mm inhibition zone. Wild honey on E.coli and Serratia marescens is sensitive in all concentrate except on the 25% concentrate. On the Klebsiella pneumonia the wild honey is sensitive on the 70%, 85%, and 100% concentrate while on the Enterobacter cloacae the wild honey is sensitive on 55%, 70%, 85% and 100% concentrate. Conclusion : wild honey is effective to inhibit bacteria growth on the positive widal blood culture of Enterobacteriaceae familia member and there are effect of various wild honey concentrate with the growth of Enterobacteriaceae bacteria which the higher concentrate of the wild honey, the greater inhibition potency towards the bacteria.

scholarly journals DIFFERENCES OF INHIBITION ZONE FOREST PURE HONEY AND CINNAMON (Cinnamomum burmani) AS ANTIBACTERIAL BACTERIAL COMPOUNDS OF MEMBERS OF THE Enterobacteriaceae FAMILY

2021 ◽  
Vol 10 (1) ◽  
pp. 7-11
Author(s):  
Eko Naning Sofyanita ◽  
Roni Afriansya
Keyword(s):  
Klebsiella Pneumoniae ◽  
Serratia Marcescens ◽  
Agar Plate ◽  
Enterobacter Cloacae ◽  
Inhibition Zone ◽  
Salmonella Typhi ◽  
Combination Method ◽  
E Coli ◽  
Esherichia Coli ◽  
Active Substances

Honey and cinnamon are plant products that have many properties, one of which is used as an antibacterial agent for various diseases. The effectiveness of the two active substances against bacteria in the Enterobacteriaeae family is unclear. The study was conducted to determine the differences in the effectiveness of forest honey and cinnamon in inhibiting the growth of bacteria in the Enterobacteriaceae family. This research method is an experimental study with a combination method of diffusion and wells using samples of the bacteria Klebsiella pneumoniae, Esherichia coli, Salmonella typhi, Serratia marcescens, and Enterobacter cloacae. Forest pure honey and cinnamon were prepared as test solutions with concentrations of 25%, 40%, 55%, 70%, 85% and 100%. The results obtained were the inhibition zone formed on Nutrient Agar Plate (NAP) media, in forest pure honey the inhibition zone formed in S. typhi, E. coli, Klebsiella pneumoniae, Serratia marcescens, and Enterobacter cloacae was greater at 100% concentration, namely 30.5mm, 32.5mm, 38mm, 29.5mm, 26mm, while the 100% concentration of cinnamon showed results, namely 12mm, 10mm, 8.5mm, 11mm, and 11mm. Conclusion is Forest pure honey was more effective at inhibiting bacteria from the Enterobacteriaceae family than cinnamon.

scholarly journals Daya Hambat Kayu Manis (Cinnamomum burmanni) terhadap Pertumbuhan Bakteri Kultur Darah Widal Positif Anggota Familia Enterobacteriaceae

2021 ◽  
Vol 3 (2) ◽  
pp. 227-230
Author(s):  
Windya Nazmatur Rahmah
Keyword(s):  
Blood Culture ◽  
Research Result ◽  
Family Members ◽  
Agar Plate ◽  
Inhibition Zone ◽  
Salmonella Typhi ◽  
Positive Control ◽  
Test Solution ◽  
Klebsiella Pneumonia ◽  
Nutrient Agar Plate

Cinnamon has a high activity of antibacterial. Still, the antibacterial effect toward the bacteria on the positive Widal blood culture of Enterobacteriaceae family members was not clear yet. This research aimed to find out the inhibition of cinnamon on the growth of bacterium on the positive Widal blood culture of Enterobacteriaceae family members. The method used in the research was experimental research which was using the draw well method and using Salmonella typhi, Escherichia coli, Klebsiella pneumonia, Serratia marcescens, and Enterobacter cloacae bacteria samples. Cinnamon as a test solution with a 2%, 4%, 6%, 8% and 10% concentrate. The research result was showed the inhibiting zone was able to form on the Nutrient Agar Plate (NAP) on growth of the Enterobacteriaceae, 10% concentrate that high sensitive showed to S. typhi, Ser. marcescens, E. cloacae, E.coli, and Kleb. pneumonia squent was 12 mm, 11 mm, 11 mm, 10 mm, and 8,5 mm. At the same time, 8%, 6%, 4%, and 2% concentrate of cinnamon could not perform inhibition zone 100 µl of the test solution with chloramphenicol as a positive control.

FUNDAMENTAL AUTARCHICAL SCREENING AND MICROBES EXERTION OF GERMANE SYLVESTRE

2021 ◽  
Author(s):  
Johns H. ◽  
Keyword(s):  
Agar Plate ◽  
Life Forms ◽  
Salmonella Typhi ◽  
National Committee ◽  
Klebsiella Pneumonia ◽  
Proteus Vulgaris ◽  
Dissemination Strategy ◽  
Chemical Laboratory ◽  
Microbial Strains

The current investigation was done to assess the autarchical and antimicrobes action of Germane sylvestre against ten microbial strains causing oral contaminations. The subjective autarchical examinations were completed after the Ukn pharmacopeia and the techniques. The MIC estimations of the plant extricates were resolved against the chose test life forms utilizing the techniques as depicted by National Committee for Chemical Laboratory Standard and the in vitro antimicrobes movement was controlled by utilizing the agar plate dissemination strategy. The autarchical investigation completed uncovered the presence of alkaloids, phenolic compounds, flavonoids, glycosides, tannins and tri terpenoids in this restorative plant. The antimicrobes movement of five distinct concentrates of therapeutic plants were assessed utilizing admirably dissemination technique against Staphylococcus aureus, Streptococcus faecalis, Escherichia coli, Pseudomonas aeruginosa, Klebsiella pneumonia, Proteus vulgaris, Salmonella typhi, Chromobacterium violaceum, Burkolderia mallei and Candida albicans separately. The chloroform concentrates of this plant shown best antimicrobes movement against chose organisms. The outcomes give defense to the utilization of the restorative plants to treat different oral contaminations.

The Role of Bacteria in Urinary Tract Infection Incidence in Students University

2019 ◽  
Vol 10 (04) ◽  
pp. 731-734
Author(s):  
Mytham Abas Makki
Keyword(s):  
Escherichia Coli ◽  
Prevalence Rate ◽  
Vibrio Parahaemolyticus ◽  
Bacterial Species ◽  
Enterobacter Cloacae ◽  
Salmonella Typhi ◽  
Klebsiella Pneumonia ◽  
Prevalence Rates ◽  
The University

This study was carried out from November 2016 to March 2017. One hundred sixty study samples were collected from the students of the university weekly, which were studied by conducting bacteriological tests on them within 24 hours from the date of collecting the sample, the results of the study showed the isolation and diagnosis of different bacterial species with varying prevalence rates. The Escherichia coli showed the highest incidence of 50% followed by Enterococcus fecalis 36.2%, Pseudomonas aeuroginosa 34.8%, Enterobacter cloacae 26.8%, Staphylococcus aureus had a prevalence rate of 22.5%. Salmonella typhi had a prevalence rate of 16.6%. Proteus mirabilis showed an average prevalence of 12%. Klebsiella pneumonia was 9.4%, while Vibrio parahaemolyticus and showed the lowest incidence of 0.7%. The results of the study showed that the total number of infected cases was 138 of the studied samples, 86.2%, distributed among 80(50%) in female infection cases, and In males (58) infection cases, a rate of 36.2%.

scholarly journals TO STUDY THE ANTIBACTERIAL ACTIVITY OF VARIOUS EXTRACTS OF CLAUSENA DENTATA (WILLD.) ROEM.

2019 ◽  
pp. 69-73
Author(s):  
ANNAMALAI MADURAM ◽  
RAJU KAMARAJ
Keyword(s):  
Antibacterial Activity ◽  
Tamil Nadu ◽  
Pathogenic Bacteria ◽  
Volatile Oil ◽  
Stem Bark ◽  
Salmonella Typhi ◽  
Chloroform Extract ◽  
Klebsiella Pneumonia ◽  
Human Pathogens ◽  
Human Pathogenic Bacteria

Objectives: The objectives of the study were to study the antibacterial activity for the various extracts of Clausena dentata against human pathogens. Clausena (Rutaceae) is a genus of about 23 species of unarmed trees and shrubs. The stem bark of C. dentata is used in veterinary medicine for the treatment of wounds and sprains. Even though C. dentata has a lot of potential medical uses, the study of microbiological properties is very scarce. Methods: The plant C. dentata was collected from Kadagaman, near Tiruvannamalai, Tamil Nadu, India, and authenticated by Centre for Advanced Study in Botany, University of Madras, Chennai. The dry powder of stem bark was extracted with hexane, chloroform, and methanol. The extracts were subjected to qualitative phytochemical screening and antibacterial activity against human pathogenic bacteria such as Escherichia coli, Salmonella Typhi, Klebsiella pneumonia, Vibrio cholerae, and Staphylococcus aureus and compared with ciprofloxacin. Results: Qualitative chemical tests revealed the presence of various phytochemicals such as alkaloids, glycosides, carbohydrate, proteins and amino acids, phytosterols, and volatile oil. The antibacterial activity result reveals that all the extracts were are more active against V. cholerae. The activity against Pseudomonas aeruginosa was mild. Conclusion: The activity against V. cholerae was comparable with that of 5 μg/mL ciprofloxacin at the concentration of C. dentata 40 μg/mL. The orders of antibacterial activity against human pathogenic bacteria are hexane, methanol, and chloroform extract of C. dentata.

scholarly journals Composition and biological assays of the leaf essential oil of Asparagus flagellaris (Kunth) Bak

2021 ◽  
Vol 7 (1) ◽  
Author(s):  
Oluwakayode O. Odeja ◽  
Michael Gabriel Ibok ◽  
Ejike O. Okpala
Keyword(s):  
Free Radical ◽  
Essential Oil ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Salmonella Typhi ◽  
Antimicrobial Activities ◽  
Free Radical Scavenging ◽  
Gas Chromatography Mass Spectrometry ◽  
Klebsiella Pneumonia ◽  
Leaf Essential Oil

Abstract Background Asparagus flagellaris leaves are ethnomedicinally used to treat syphilis, gonorrhea and other sexually transmitted diseases (STDs), with no reports on the volatile constituents. This study was aimed to quantitatively and qualitatively characterise the composition of essential oil, evaluates the free radical scavenging and antimicrobial capacity of the essential oil. Methods The essential oil was isolated by hydrodistillation method using all-glass Clevenger-type apparatus, while the identification and quantification of constituents were performed by gas chromatography-mass spectrometry (GC-MS) technique. The antioxidant activity on 2, 2-diphenyl-1-picrylhydrazyl (DPPH) was evaluated and the oil was also tested against 10 strains of microorganisms consisting of 6 bacteria: Escherichia coli, Salmonella typhi, Klebsiella pneumonia, Pseudomonas aeruginosa, Staphylococcus aureus and Bacillus subtilis and 4 fungi: Candida albicans, Penicillium notatum, Aspergillus niger and Rhizopus spp. using broth dilution and surface plate methods, respectively. Results A pale yellow essential oil with a characteristic scent was obtained, with a yield of 0.80% (w/w). A total of 28 compounds accounting for 97.41% of the total oil contents were identified. The oil was predominated by Thymol and its derivatives, accounting for 57.48%. The most abundance (% area) constituents of the essential oil were 5-Thymyl tiglate (18.49%), Thymyl-2-methyl butyrate (17.34%), Thymol hydroquinone dimethyl ether (10.52%), Thymol methyl ether (9.42%) and 5-Propyl-1, 3-benzodioxole (4.59%). The essential oil showed a significant free radical scavenging activity compared to the standard antioxidant drugs used in this study, with % inhibition varying from 88.06 ± 0.0001 to 93.05 ± 0.0006. The leaf essential oil exhibited antimicrobial activity on all the tested organisms at 500–125 μg/mL, with an 18–10 mm inhibitory zone. Conclusion The leaf essential oil of A. flagellaris contains notable chemical compounds responsible for its antioxidant and antimicrobial activities.

scholarly journals Stool shedding of Salmonella Typhi in children with blood culture-confirmed typhoid fever

2020 ◽  
Vol 101 ◽  
pp. 123
Author(s):  
M. Srinivasan ◽  
S. Giri ◽  
S. Kulandaipalayam Natarajan ◽  
N. Kumar ◽  
V.R. Mohan ◽  
...  
Keyword(s):  
Blood Culture ◽  
Typhoid Fever ◽  
Salmonella Typhi

Bioactivity of Some Natural and Semisynthetic Coumarin Derived Compounds

2021 ◽  
Vol 19 (6) ◽  
pp. 129-138
Author(s):  
Yasser Fakri Mustafa ◽  
Moath Kahtan Bashir ◽  
Mahmood Khudhayer Oglah ◽  
Raghad Riyadh Khalil ◽  
Eman Tareq Mohammed
Keyword(s):  
Pathogenic Fungi ◽  
Salmonella Typhi ◽  
Positive Influence ◽  
Shigella Dysenteriae ◽  
Klebsiella Pneumonia ◽  
Bacterial Strains ◽  
Cancerous Cell ◽  
Free Hydroxyl ◽  
Antibacterial And Antifungal ◽  
Diffusion Assay

A couple of natural coumarins was identified in the seeds of two apples’ cultivars commonly known as Granny Smith and Red Delicious. The effect of the phenolic hydroxyl moieties found in these products was evaluated on the bioactivity. This evaluation included the structural alteration of these moieties into less hydrophilic ones to explore the significance of the parent moieties on the biological activity. The investigated biopotentials were antioxidant, antiproliferative, antibacterial, and antifungal effects. The antioxidant potential was investigated by detecting the ability of the natural and semisynthetic coumarins to trap the free hydroxyl and DPPH radicals. The antiproliferative potential was assessed via an MTT-depended assay versus eight cancerous-cell lines, included HeLa, SK-OV-3, AR42J, MCF-7, AB12, KYSE-30, LC540, and AMN3. The antibacterial potential was tested versus six common pathogenic bacterial strains via a well-defined disc diffusion assay. These pathogens were Escherichia coli, Salmonella typhi, Klebsiella pneumonia, Haemophilus influenzae, Shigella dysenteriae, and Pseudomonas aeruginosa. The antifungal potential was also screened by utilizing a similar microbiological technique versus three pathogenic fungi, involved Candida albicans, Aspergillus flavus, and Aspergillus niger. It is concluded that the investigated chemical moiety has a positive influence on the antioxidant and antiproliferative potentials of the natural derivatives, and a negative one on their antibacterial and antifungal potentials.

scholarly journals AKTIVITAS ANTIMIKROBA YOGURT BERBASIS AIR KELAPA MENGHAMBAT BAKTERI PATOGEN SECARA IN VITRO

1970 ◽  
Vol 17 (2) ◽  
Author(s):  
Lindawati S. A. ◽  
Haniyah Y. S. ◽  
Miwada I N. S. ◽  
Inggriati N. W. T. ◽  
Hartawan M. ◽  
...  
Keyword(s):  
Salmonella Typhi ◽  
Lactobacillus Bulgaricus ◽  
Plate Count ◽  
Klebsiella Pneumonia ◽  
E Coli ◽  
Total Plate Count

Yogurt merupakan minuman susu yang difermentasi dengan menggunakan bakteri asam laktat Lactobacillus bulgaricus dan Streptococcus thermophyllus sebagai starter. Tujuan penelitian untuk mengetahui kemampuan tertinggi dari yogurt berbasis air kelapa dalam menghambat pertumbuhan bakteri pathogen (Salmonella, Staphylococcus, Klebsiela pneumonia dan E.coli) secara in vitro. Penelitian ini menggunakan rancangan acak lengkap (RAL) dengan empat perlakuan dan empat ulangan. Keempat perlakuan tersebut: Ko: susu skim 12%+air; K1: susu skim 12%+air kelapa gading; K2: susu skim 12%+kelapa gading bulan; K3: susu skim 12%+air kelapa hijau. Peubah yang diamati adalah aktivitas antimikroba yogurt terhadap bakteri pathogen (Salmonellatyphi, Staphylococcus, Klebsiella pneumonia dan E.coli), total plate count (TPC), total bakteri asam laktat dan E,coli. Hasil penelitian menunjukkan bahwa aktivitas antimikroba tertinggi diperoleh pada yogurt berbasis air kelapa bulan terhadap bakeri Salmonella typhi dan Staphylococcus masing-masing sebesar 0,21 dan 0,26 cm dengan total bakteri asam laktat1,1×107 cfu/mL sedangkan untuk Klebsiella pneumonia dan E.coli diperoleh pada yogurt berbasis air kelapa gading sebesar 0,38 dan 0,64 mm dengan total bakteri asam laktat 3,1×106 cfu/mL.Dari hasil penelitian dapat disimpulkan bahwa yogurt berbasis air kelapa gading, bulan, hijau dan tanpa air kelapa memiliki aktivitas antimikroba berspektrum luas (menghambat bakteri gram positif dan negatif).

scholarly journals EFFECTIVITY ANTIBACTERIAL ZINC OXIDE EUGENOL WITH ZINC OXIDE PROPOLIS FOR ENDODONTIC TREATMENT IN PRIMARY TEETH

2019 ◽  
Vol 6 (2) ◽  
pp. 113
Author(s):  
Erwid Fatchur Rahman ◽  
Sandy Christiono
Keyword(s):  
Zinc Oxide ◽  
Enterococcus Faecalis ◽  
Agar Plate ◽  
Inhibition Zone ◽  
Blood Agar Plate ◽  
Control Group ◽  
Control Group Design ◽  
Normality Test ◽  
Zinc Oxide Eugenol ◽  
Post Test

Background: Enterococcus faecalis is generally found on the failure of root canal treatments. Zinc oxide propolis is believed to have an antibacterial effect on that bacteria. This research aimed to compare bacteriostatic effect of zinc oxide eugenol (ZOE) and zinc oxide propolis (ZOP) as the sealer materials of root canal.Method: This was an experimental research with post-test only control group design with two different groups (ZOE and ZOP). Culture of Enterococcus faecalis bacteria was smeared on Blood Agar Plate media with six times replication per group and kept inside incubator for 24 hours. The result was obtained from the inhibition zone formed around the pasta.Result: The average result of ZOE and ZOP was 27.7 mm and 13.45 mm respectively. Normality test using Shapiro-Wilks showed that data was normal (p>0.05). Then, the data was analysed using Independent Samples T-test. The result showed that there was different inhibition zone between ZOE group and ZOP group (p<0.05).Conclusion: Based on the result, it can be concluded that ZOP has lower antibacterial effectiveness of the Enterococcus faecalis than ZOE.

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