scholarly journals ACCURATE DETECTION OF VIRAL SEROTYPE DENGUE HEMORRHAGIC FEVER THROUGH AEDES SP MOSQUITOES USING REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION (RT-PCR)

2021 ◽  
Vol 10 (2) ◽  
pp. 148-152
Author(s):  
Erlin Yustin Tatontos ◽  
Fihiruddin Fihiruddin ◽  
Nurul Inayati

Dengue Hemorrhagic Fever (DHF) caused Dengue virus which transmitted through the bite of the Aedes sp. Detection of dengue virus serotypes is essential for epidemiology as well as potential disease pathogens. RT-PCR method was more effective in mosquitoes, because the virus contained in the mosquito body is passed on to the next generation (trans ovarian). The purpose of this research is to know the serotype of DHF virus accurately through vector mosquito using RT-PCR method in Mataram City, so that vector control, early diagnosis and management of DHF disease could be done quickly and precisely. This research was a laboratory explorative research with cross sectional design that determines serotype of DHF virus through vector mosquito using RT-PCR method in Mataram City. The population of this study were Aedes aegypti female mosquitoes from eggs. Collected from houses located in Community Health Center Mataram city worked area with high dengue cases: Pagesangan, Karang Taliwang, Karang Pule, Tanjung Karang, Mataram, and Selaparang especially around the house’s sufferers. The results showed that only the mosquito group from the worked area of the Mataram Community Health Center showed the ribbon image with the base pair in accordance with serotype 1 (DENGUE 1), the other negative.

2017 ◽  
Vol 9 (1) ◽  
pp. 56 ◽  
Author(s):  
Siwi Pramatama Mars Wijayanti ◽  
Dian Anandari ◽  
Arrum Firda Ayu Maghfiroh

Mosquito density could predict the risk of Dengue Virus (DENV) transmission in certain area. Ovitrap Index (OI) is one of the methods to measure the mosquito density beside other methods such as Stegomyia Indices and free larvae index. Ovitrap index is cheaper, more appliacable and sensitive to detect Aedes sp activity to lay their eggs on the wall of container. The aims of this research were to measure ovitrap index in endemic DENV areas, to find out the preferences of female Aedes sp to lay their eggs, and find out the correlation between OI and dengue cases in endemic DENV areas. Type of this study was decriptive with cross sectional design. The area of study was in Community Health center Purwokerto Timur II in Kranji, Sokanegara and Purwokerto Lor. Ovitrap were installed at 50 houses of each vilagges (one indoor, one outdoor), with total ovitrap 100 in each villages. After 6 days of installation, ovistrips were collected and the eggs were counted. The Ovitrap index in three villages were observed at percentage more than 50%, with the highest OI detected in Kranji Village (97.56%). Female Aedes sp were monitored to lay their eggs outdoor than indoor. The OI is not always relevant to predict the dengue cases but could inform the risk of DENV tranmission in certain area. The community should do efforts to minimize potential breeding sites, mainly unused container outdoor to reduce the mosquito density.


2017 ◽  
Vol 9 (01) ◽  
pp. 47 ◽  
Author(s):  
Siwi Pramatama Mars Wijayanti ◽  
Dian Anandari ◽  
Arrum Firda Ayu Maghfiroh

Mosquito density could predict the risk of Dengue Virus (DENV) transmission in certain area. Ovitrap Index (OI) is one of the methods to measure the mosquito density beside other methods such as Stegomyia Indices and free larvae index. Ovitrap index is cheaper, more appliacable and sensitive to detect Aedes sp activity to lay their eggs on the wall of container. The aims of this research were to measure ovitrap index in endemic DENV areas, to find out the preferences of female Aedes sp to lay their eggs, and find out the correlation between OI and dengue cases in endemic DENV areas. Type of this study was decriptive with cross sectional design. The area of study was in Community Health center Purwokerto Timur II in Kranji, Sokanegara and Purwokerto Lor. Ovitrap were installed at 50 houses of each vilagges (one indoor, one outdoor), with total ovitrap 100 in each villages. After 6 days of installation, ovistrips were collected and the eggs were counted. The Ovitrap index in three villages were observed at percentage more than  50%, with the highest OI detected in Kranji Village (97.56%). Female Aedes sp were monitored to lay their eggs outdoor than indoor. The OI is not always relevant to predict the dengue cases but could inform the risk of DENV tranmission in certain area. The community should do efforts to minimize potential breeding sites, mainly unused container outdoor to reduce the mosquito density.


2018 ◽  
Vol 6 (1) ◽  
Author(s):  
Erike Yunicha Viridula

ABSTRAKGangguan pertumbuhan bayi pada usia dini menyebabkan bayi diberikan MP-ASI terlalu dini dan ibunya tidak memberikan ASI eksklusif selama 6 bulan pada awal kehidupan bayi. Dari hasil studi pendahuluan pada bayi berusia 6-11 bulan yang mendapatkan ASI eksklusif hampir sepenuhnya meragukan perkembangan. Jika ini terus berlanjut, bayi tidak dapat berkembang dengan baik di usia berikutnya. Tujuan penelitian ini untuk mengetahui perbedaan perkembangan pada bayi usia 6-11 bulan yang mendapat ASI eksklusif dan ASI non eksklusif di Puskesmas kecamatan kecamatan Durenan Trenggalek tahun 2017.Desain penelitian ini adalah studi inferensial dengan pendekatan cross sectional. Sampel yang diambil dalam penelitian ini adalah ibu dengan bayi 6-11 bulan pada bulan Oktober di Puskesmas kecamatan kecamatan Durenan Trenggalek pada tahun 2017 berjumlah 40 responden yang diambil menggunakan metode probability sampling dari jenis sistem simple random sampling. Data dianalisis dengan analisis univariat bahwa distribusi frekuensi variabel independen dan dependen dan analisis bivariat menggunakan Mann-Whitney U.Hasil penelitian menunjukkan bahwa dari 20 responden 95% yang mendapatkan ASI eksklusif memiliki perkembangan yang sesuai, 5% memiliki perkembangan yang meragukan, 0% mengalami gangguan perkembangan. Sementara 20 responden diberi 40% menyusui non eksklusif memiliki perkembangan yang sesuai, 60% memiliki perkembangan yang meragukan, dan 0% mengalami gangguan perkembangan. Hasil analisis statistik menunjukkan ada perbedaan perkembangan pada bayi usia 6-11 bulan yang mendapat ASI eksklusif dan ASI non eksklusif di Puskesmas kecamatan Durenan Trenggalek tahun 2017.Jadi dapat disimpulkan bahwa ada perbedaan perkembangan pada bayi usia 6-11 bulan yang mendapatkan ASI eksklusif dan ASI non eksklusif di Puskesmas kecamatan Durenan Trenggalek tahun 2017. Sehingga diharapkan bayi mendapat ASI eksklusif selama 6 bulan pertama dan dilanjutkan hingga 2 tahun dengan makanan pendamping (MP-ASI).Kata kunci : ASI eksklusif, ASI tidak eksklusif, pengembangan ABSTRACTDisorder of Infant growth in early life caused baby are given Complementary feeding (MP-ASI) too early and the mother didn’t give exclusive breastfeeding for 6 months in early life of baby. From the results of preliminary studies in infants aged 6-11 months who get non exclusive breastfeeding had almost entirely dubious developments. If this continues, the baby can’t develop properly in the next age. The purpose of this study to determine development differences in infants aged 6-11 months who get exclusive breastfeeding  and non- exclusive breasfeeding in community health center sub district Durenan Trenggalek district in 2017. The design of this study is inferential study with cross sectional approach.  Samples which taken in this study were mothers with infants 6-11 months in October in community health center sub district Durenan Trenggalek district in 2017 amounted to 40 respondents drawn using probability sampling method of the type system simple random sampling.  Data were analyzed by univariate analysis that the frequency distribution of independent and dependent variables and bivariate analysis using Mann-Whitney U. The results showed that of the 20 respondents 95% who get exclusive breastfeding had appropriate development, 5% had dubious developments, 0% had disorder development. While 20 respondents were given 40% non exclusive breastfeeding had appropriate development, 60% have dubious development, and 0% had disorder development. Statistical analysis showed there are differences development in infants aged 6-11 months who get exclusive breastfeeding and non-exclusive breasfeeding in community health center sub district Durenan Trenggalek in 2017. So can be concluded that there are differences development in infants aged 6-11 months who get exclusive breastfeeding and non-exclusive breasfeeding in community health center sub district Durenan Trenggalek in 2017.So expected the babies got exclusive breastfeeding for the first 6 months and continued to 2 years with complementary foods (MP-ASI).Key Words : exclusive breastfeding, non exclusive breastfeding, development


2018 ◽  
Vol 3 (3) ◽  
pp. 568
Author(s):  
Ainal Mardiah ◽  
Arni Amir ◽  
Andi Friadi ◽  
Ellyza Nasrul

<p><em>Iron deficiency anemia is anemia caused by iron deficiency in the blood. Maternal iron deficiency affects the low iron reserves in neonates </em><em>and it also influences on </em><em>Brain Derived Neurotropic Factor (BDNF) </em><em> which affects cognitive function.</em><em> </em><em>The purpose of this study was to determine the difference mean of BDNF in neonates from normal pregnant women and pregnant women with iron deficiency. </em><em>The design of this research was Cross Sectional</em><em> </em><em>design. This research was conducted in Community Health Center of Lubuk  Buaya, Ambacang Community Health Center, Community Health Center of Ikur Koto Health Center and Biomedical Laboratory of Andalas University on February 2017 to April 2018. There were 42 pregnant women was selected as sample e of this research. The samples were chosen by Consecutive Sampling. Then, the sample is divided into two groups: normal pregnant women and pregnant women with iron deficiency anemia. BDNF are examined by the ELISA. Next, the data were analyzed by using T test. The levels of BDNF neonates in normal pregnant group was 3.65(ng/ml) and the anemia pregnant group was 1.74(ng/ml) (p &lt;0.05). There was significant difference of BDNF levels in neonates from normal pregnant women and pregnant women with iron deficiency anemia. </em><em>The conclusion of this study is there is a difference of average BDNF in neonates from normal pregnant women and pregnant women with iron deficiency.</em></p><p> </p><p>Anemia defisiensi besi adalah anemia yang disebabkan karena kekurangan zat besi dalam darah. Defisiensi besi  maternal berdampak pada rendahnya cadangan besi pada neonatus dan berdampak terhadap ekspresi Brain Derived Neurotropic Factor (BDNF) yang berpengaruh pada fungsi kognitif. Tujuan penelitian ini adalah untuk mengetahui perbedaan rerata kadar BDNF pada neonatus dari ibu hamil normal dan ibu hamil defisiensi besi. Desain penelitian ini adalah Cross Sectional. Penelitian dilakukan di Puskesmas Lubuk Buaya, Puskesmas Ambacang, Puskesmas Ikur Koto dan Laboratorium Biomedik Universitas Andalas pada bulan Februari 2017 – Juli 2018. Sampel Penelitian adalah ibu hamil sebanyak 42 orang yang dipilih secara Consecutive Sampling, sampel dibagi menjadi dua kelompok yaitu ibu hamil normal dan ibu hamil anemia defisiensi besi. BDNF diperiksa dengan metode ELISA. Data dianalisa menggunakan uji T test. Kadar BDNF neonatus pada ibu kelompok normal adalah 3,65(ng/ml) dan kelompok ibu anemia adalah 1,74(ng/ml) (p&lt;0,05). Terdapat perbedaan bermakna kadar BDNF pada neonatus dari ibu hamil normal dan ibu anemia defisiensi besi. <em></em></p>


2022 ◽  
Vol 3 (1) ◽  
pp. 202-208
Author(s):  
Balqis Dwiyanti Haedar ◽  
Rauly Ramadhani ◽  
Andi Sitti Rahma

Exclusive breastfeeding is a condition in which infants are only given breast milk without the addition of other fluids and foods. until the baby reaches 6 months old. Breast milk strengthen the immune system in infants so they can avoid various diseases including diarrhea. This study aims to determine the relationship between exclusive breastfeeding and the incidence of diarrhea in infants aged 0-6 months in the Sudiang Community Health Center. This research is an analytical survey research with a cross-sectional design. This research was conducted at the Sudiang Health Center, Makassar City in the period November 1st to December 31st, 2019. The total respondents were 89 mothers with babies aged 0-6 months. The results showed that most of the respondents were mothers with male babies (61%), most of the mothers' education was high school graduates (46%), housewives (75%) with low economic background (56%). Exclusive breastfeeding related to the incidence of diarrhea in children aged 0-6 months in the Sudiang Community Health Center with a value of p <0.05 (p = 0.001). In addition, no significant relationship on maternal nipple hygiene to diarrhea in children aged 0-6 months p <0.05 (p = 0.075). In conclusion, this study showed there is a relationship between exclusive breastfeeding and the incidence of diarrhea in children aged 0-6 months in Sudiang Health Center. There is a relationship between hand hygiene and the incidence of diarrhea in children aged 0-6 months in Sudiang Health Center, Makassar.


Author(s):  
Wahyuni Herda ◽  
Insan Sosiawan A Tunru ◽  
Yusnita Yusnita

Tuberculosis is a disease of global concern. By 2015 six countries contributing to 60% of the global total are India, Indonesia, China, Nigeria, Pakistan and South Africa. China, India and Indonesia alone accounted for 45% of cases in the world.The World Health Organization (WHO)has recommended the Directly Observed Treatment Shortcourse (DOTS) strategy for TB control by involving Drug Supervisors (PMO). It aims to achieve patient recovery, prevent transmission, and avoid drug resistant cases. This study aims to determine the relation between the roles of treatment observers (PMO) with the success of tuberculosis treatment at community health center of Johar Baru Central Jakarta in 2016.This research was conducted by Cross-Sectional non-experimental quantitative method. Population and sample are the patient of adult pulmonary and extrapulmonary tuberculosis at community health center of Johar Baru Central Jakarta in 2016. Samples are selected by using Simple Random Sampling. The data were collected by interview using questionnaire. Data analysis using SPSS with Chi-Square test.There were 45 respondents (80,4%) succeed in TB treatment and respondent with PMO roles category were 40 (71,4%). Result of statistical test using Chi-Square test obtained P value = 1,000 (> 0,05).There is no relation between the roles of treatment observers (PMO) with the success of tuberculosis treatment at community health center of Johar Baru Central Jakarta in 2016.


2018 ◽  
Vol 25 (1) ◽  
pp. 9-17
Author(s):  
Nurul Khairani ◽  
Santoso Ujang Effendi ◽  
Desi Kurnia Putri

This study was based on the still high nutrient deficiency of  elderly in                  the working area of  Bentiring  Community  Health  Center   Bengkulu. This study aimed to study the relationship between education and knowledge with                        nutritional  status of  elderly in  Elderly  Integrated  Service  Post  working  area  of   Bentiring  Community  Health  Center Bengkulu. The  research type was Analytical Survey and the desain of research was Cross Sectional. The population of  this  study  was  all  elderly who visited  Elderly Integrated  Service Post                in  the  working  area  of Bentiring Community  Health  Center  Bengkulu                   in January to April 2017 which amounted to 52 elderly. Sampling technique in this research used   Total  Sampling. The data collected in this study used primary data and secondary data with data analysis technique done by univariate and bivariate analysis with Chi-Square test (c²) through SPSS 16 program. The result of  this research were : from 52 elderly,  there were  35 elderly (67,3%)  with basic education, 27 elderly  (51,9%)  who had  less knowledge, 20 elderly  (38,5%)  had  less  nutritional  status, there was significant relationship  between  education  with  nutritional  status of  elderly  with  medium category, and there was a significant relationship between knowledge with nutritional status  of  elderly with close category. It was expected to Bentiring Community Health Center Bengkulu to improve health services to the elderly by providing nutrition education and medical  examination  for  elderly.  Keywords : education, elderly, knowledge, nutritional status, elderly


2018 ◽  
Vol 12 (02) ◽  
Author(s):  
Abdul Malik Lawira

ABSTRACTThis research aims to identify factors that can effect the behavior of mothers during exclusive breastfeeding for infants aged 0-6 months in Malei Community Health Center in Poso. The design of this research is observational analytic with Cross Sectional design. The sample of 82 respondents, who were the total of population, consisted of mothers who had babies aged 6-12 months. Data collection tool was questionnaires and techniques of data analysis were univariate data and bivariate data. The results showed age factors (p = 0.158), education (p = 0.360) and employment (p = 0.543), did not have a meaningful relationship on exclusive breastfeeding behavior. While the duration of breastfeeding factors (p = 0.007), family support (p = 0.005) and knowledge (p = 0.005), have a significant relationship with exclusive breastfeeding behavior in Malei Community Health Center, Poso. Conclusions, the duration of breastfeeding factors, family support and knowledge, have a relationship towards mother's exclusive breastfeeding behavior in Malei Community Health Center, Poso Regency. Thus, this study suggests to increase family support and duration of breastfeeding for each mother and providing knowledge about exclusive breastfeeding for antenatal activities and postnatal lactation support, both at the hospital and at the community health center up to six months after giving birth.Keywords: Duration of breastfeeding, Family support, knowledge, Exclusive breastfeeding


2020 ◽  
Vol 2 (2) ◽  
pp. 15-23
Author(s):  
Rusmini Marslan Arsyad ◽  
Engelina Nabuasa ◽  
Enjelita M. Ndoen

DHF is one of the widespread  infectious diseases in Indonesia, with an increased infected number of sufferers. DHF case is closely related to environmental sanitation, wich causes the availability of breeding places for the Aedes aegypti mosquito vectors. The study was to determine the relationship between environmental sanitation behavior and the case of DHF in the working area of the Tarus Community Health Center in 2020. The study design was descriptive-analytical with a cross-sectional study approach. The sample was_99 respondents taken by simple random sampling technique. Data was collected from interviews and analyzed using the Chi-square test. The results showed that the varuabels of  knowledge (p = 0.000), attitudes (p = 0.021), and actions to environmental sanitation (p = 0.000) were related to the DHF case. The Tarus Community Health Center should increase outreach activities and family empowerment efforts related to the prevention and control of DHF.


2020 ◽  
Vol 7 (1) ◽  
pp. 16
Author(s):  
Nita Pujianti ◽  
Lia Anggraini

ABSTRAKPneumonia merupakan penyebab banyak kematian balita di dunia. Selama tahun 2016 terdapat 568.146 (65,27%) jumlah kasus pneumonia pada balita yang ada di Indonesia. Berdasarkan data Dinas Kesehatan Provinsi Kalimantan Selatan tercatat kasus pneumonia tahun 2017 sebesar 66,52%, data tertinggi pada Puskesmas Beruntung Raya dengan angka 105 kasus (10,80%) di tahun 2017. Tujuan penelitian ini adalah untuk menganalisis faktor-faktor yang berhubungan dengan kepatuhan penggunaan antibiotika pada orang tua pasien anak yang terdiagnosa pneumonia di wilayah kerja Puskesmas Beruntung Raya serta menganalisa faktor yang paling dominan mempengaruhi. Penelitian ini menggunakan rancangan observasional analitik melalui pendekatan cross sectional. Populasi dalam penelitian ini adalah semua orang tua yang memeriksakan anaknya dalam satu tahun terakhir di Puskesmas Beruntung Raya. Sampel dalam penelitian ini berjumlah 77 responden ditentukan dengan teknik purposive sampling dan berlangsung selama 3 (tiga) bulan. Analisa data menggunakan uji chi square dan regresi logistik biner dan kuisioner sebagai Instrumen penelitian. Hasil penelitian menunjukkan bahwa terdapat hubungan antara kedisiplinan (p-value= 0,001), sugesti sembuh (p-value= 0,012), dan komunikasi (p-value= 0,025). Variabel kedisiplinan merupakan yang paling berpengaruh dominan terhadap kepatuhan penggunaan antibiotika, sehingga pasien yang disiplin lakan lebih patuh dalam meminum obat antibiotika daripada pasien yang tidak disiplin.Kata-kata kunci: Kepatuhan, pneumonia, kedisiplinan, sugesti sembuh, komunikasiABSTRACTPneumonia is the cause of many under-five deaths in the world. During 2016 there were 568,146 (65.27%) of the number of pneumonia cases in children under five in Indonesia. From the data of the South Kalimantan Provincial Health Office recorded pneumonia cases in 2017 amounted to 66.52%, the highest data in Beruntung Jaya Community Health Center with a number of 105 cases (10.80%) in 2017. The purpose of this study was to analyze the factors related by adhering to the use of antibiotics in the parents of pediatric patients with diagnosed with pneumonia in the work area of Beruntung Jaya Community Health Center and analysis the most dominant factor influencing. This study uses an observational analytic design through a cross sectional approach. The population in this study were all parents who had their children examined in the past year at Beruntung Raya Health Center. The sample in this study amounted to 77 respondents determined by purposive sampling technique and lasted for 3 (three) months. Data analysis using chi square test and binary logistic regression and questionnaires as research instruments. The results showed that there was a relationship between discipline (p-value= 0.001), recovery suggestions (p-value= 0.012), and communication (p-value= 0.025). Disciplinary variable is the most dominant influence on adherence to the use of antibiotics, so that disciplined patients are more obedient in taking antibiotic drugs than patients who are not disciplined.Key words: Compliance, pneumonia, discipline, healing suggestion, communication


Sign in / Sign up

Export Citation Format

Share Document