UHPLC, ATR-FTIR analysis of Nymphoides indica rhizome extract and determination of antioxidant & antibiofilm potential

2021 ◽  
pp. 1-10
Author(s):  
Muhammad Hanif ◽  
Muzammil Ahmad Khan ◽  
Abdul Rafey ◽  
Imran Aziz ◽  
Mohsin Ali Khan ◽  
...  
Keyword(s):  
Klebsiella Pneumoniae ◽  
Ethyl Acetate ◽  
Biofilm Formation ◽  
Biological Activities ◽  
Significant Inhibition ◽  
Phytochemical Analysis ◽  
Ftir Analysis ◽  
Antioxidant Assay ◽  
Nymphoides Indica

The aim of the study was to perform comprehensive phytochemical analysis, UHPLC, ATR-FTIR profiling and evaluation of various biological activities of N. Indica rhizome. The phytochemical analysis indicated presence of alkaloids, saponins, and triterpenes. In the antioxidant assay, the methanolic (IC50 40.3±0.04) and chloroform fractions (IC50 40.05±0.21) showed highest DPPH inhition. Like wise, methanolic fraction showed highest FRAP value (756.2±0.06) followed by chloroform (225.0±0.04) and ethyl acetate fractions (193.0±0.21). In the antimicrobial assays, chloroform (MIC <  0.156 mg/ml) and methanol fractions (MIC 0.625 mg/ml) fractions showed significant inhibition of Klebsiella pneumoniae. Also a significant antibiofilm of biofilm formation was recorded by chloroform (IC50 1.73 mg/ml) and ethyl acetate fractions (IC50 1.76 mg/ml). It was concluded that the N. Indica rhizome posess antioxidant, antimicrobial and antibiofilm potential, that may be attributed to high flavonoid contents.

scholarly journals Phytochemical Content, Antibacterial and Antioxidant Potential of Endemic Plant Anabasis aretioïdes Coss. & Moq. (Chenopodiaceae)

2020 ◽  
Vol 2020 ◽  
pp. 1-16
Author(s):  
Souad Senhaji ◽  
Fatima Lamchouri ◽  
Hamid Toufik
Keyword(s):  
Antioxidant Activity ◽  
Quantitative Analysis ◽  
Ethyl Acetate ◽  
Ethyl Acetate Extract ◽  
Biological Activities ◽  
Diffusion Method ◽  
Total Phenolic ◽  
Phytochemical Analysis ◽  
Endemic Plant

This study aims to investigate the biological activities of endemic plant Anabasis aretioïdes harvested in eastern Morocco. Various extracts were prepared from aerial part by aqueous and organic extraction using a Soxhlet and cold maceration. Preliminary phytochemical analysis was carried out on the powder and on the different extracts by standard phytochemical tests, and was confirmed by a quantitative analysis based on the determination of total polyphenols and cathechic tannins. Antioxidant activity was evaluated in vitro by five methods: H2O2, DPPH, ABTS, FRAP, and RP, and the antibacterial activity was carried out by disc diffusion method and the determination of MIC and MBC. Phytochemical screening revealed the presence of cathechic tannins, saponins, and sterols and quantitative analysis showed that Ethyl acetate extract presented the high level of phenolic and cathechic tannins contents (46.79 ± 0.75 µg GAE/mg E and 46.46 ± 0.67 µg CE/mg E). A highest hydrogen peroxide activity was observed in aqueous macerated extract (7.84 ± 0.44%) and the macerated methanol extract has the highest rates for the other four antioxidant activity tests: It was able to reduce DPPH with an IC50 of 52.91 ± 0.24 µg/ml, the highest ABTS•+ radical scavenging capacity (48.99 ± 1.316 µg TE/mg E), it showed also the highest antioxidant activity by the FRAP and reducing power test (99.73 ± 3.570 µg TE/mg E and 72.176 ± 0.540 µg AAE/mg E). Antibacterial screening showed that the maximum zone of inhibition was noted for ethyl acetate extract against Staphylococcus aureus (13.5 mm). The lowest MIC value was obtained with methanolic and macerated methanolic extracts against Protéus mirabilis strain (MIC = 3.125 mg/ml). Principal component analysis showed that the four methods ABTS, DPPH, FRAP, and RP are highly correlated and a correlation between the antioxidant activity and the total phenolic contents of the extracts indicated that phenolic compounds were the dominant contributors to the antioxidant activity of the plant.

scholarly journals Composition Analysis and Inhibitory Effect ofSterculia lychnophoraagainst Biofilm Formation byStreptococcus mutans

2016 ◽  
Vol 2016 ◽  
pp. 1-9 ◽  
Author(s):  
Yang Yang ◽  
Bok-Im Park ◽  
Eun-Hee Hwang ◽  
Yong-Ouk You
Keyword(s):  
Biofilm Formation ◽  
Ethanol Extract ◽  
Inhibitory Effect ◽  
Significant Inhibition ◽  
Control Group ◽  
Composition Analysis ◽  
Phytochemical Analysis ◽  
Chinese Drug ◽  
Nutrient Contents ◽  
Ethanol Extracts

Pangdahai is a traditional Chinese drug, specifically described in the Chinese Pharmacopoeia as the seeds ofSterculia lychnophoraHance. Here, we separatedS. lychnophorahusk and kernel, analyzed the nutrient contents, and investigated the inhibitory effects ofS. lychnophoraethanol extracts on cariogenic properties ofStreptococcus mutans, important bacteria in dental caries and plaque formation. Ethanol extracts ofS. lychnophorashowed dose-dependent antibacterial activity againstS. mutanswith significant inhibition at concentrations higher than 0.01 mg/mL compared with the control group (p<0.05). Furthermore, biofilm formation was decreased byS. lychnophoraat concentrations > 0.03 mg/mL, while bacterial viability was decreased dose-dependently at high concentrations (0.04, 0.08, 0.16, and 0.32 mg/mL). Preliminary phytochemical analysis of the ethanol extract revealed a strong presence of alkaloid, phenolics, glycosides, and peptides while the presence of steroids, terpenoids, flavonoids, and organic acids was low. TheS. lychnophorahusk had higher moisture and ash content than the kernel, while the protein and fat content of the husk were lower (p<0.05) than those of the kernel. These results indicate thatS. lychnophoramay have antibacterial effects againstS. mutans, which are likely related to the alkaloid, phenolics, glycosides, and peptides, the major components ofS. lychnophora.

scholarly journals Study on biological activities of extracted fractions from Typhonium flagelliforme (Lodd.) Blume

2017 ◽  
Vol 33 (2S) ◽  
Author(s):  
Le Quy Thuong ◽  
Bach Tuyet Mai ◽  
Nguyen Minh Chau ◽  
Le Thi Phuong Hoa ◽  
Nguyen Quang Huy
Keyword(s):  
Ethyl Acetate ◽  
Cytotoxic Activity ◽  
Biological Activities ◽  
Ethyl Acetate Fraction ◽  
Positive Control ◽  
Chemical Compositions ◽  
Active Components ◽  
Stomach Pain ◽  
Ic50 Values

Typhonium flagelliforme is a medicinal plant that has variety of uses. In medicinal traditional T. flagelliforme is used to treatment cough, headache, stomach pain chronic, and tracheitis. Moreover, use fresh bulbs treatment furuncle, the bites of poisonous insects. The active components in T. flagelliforme are flavonoids. In this study, the T. flagelliforme extract was obtained by methanol  to determine the chemical composition. Then, The extracts of methanol are extracted with polarization increases gradually solvents such as haxane, dichloromethane and ethyl acetate. Determination of antioxidant activity, cytotoxic activity of extracted fractions. Results obtained showed that the chemical compositions by the qualitative reaction preliminary were identified from T. flagelliforme containing reducing sugars, amino acids, organic acids, flavonoids, alcaloids, sterols. The antioxidant capacity of the ethyl acetate fraction reached 94.76 μg/ml, 10 times higher than the positive control is Quercetin. Cytotoxic activity of the haxane and diclomethane extracted fractions from T. flagelliforme exhibited cytotoxic activity on all three experimental cancers cell lines: KB, HepG2, Lu after 72h of culture with IC50 values ​​range from 92.8 to 107.76 μg/ml. From dichlomethane extracted of T. flagelliforme was purified TF1 as Stigmast-4-en- 3-on.    

scholarly journals Determination of Biological Activities of the Root Bark of Senna singueana

2020 ◽  
pp. 25-34
Author(s):  
Kidanemariam Teklay Hilawea ◽  
Zelalem Yibralign Desta
Keyword(s):  
Antibacterial Activity ◽  
Biological Activities ◽  
Total Flavonoid ◽  
Diffusion Method ◽  
Total Phenolic ◽  
Phytochemical Analysis ◽  
Root Bark ◽  
Total Flavonoid Content ◽  
Flavonoid Content

Aims: Senna singueana is a medicinal plant which is used for the treatment of different kinds of diseases and the plant was selected for the study because of its numerous uses. The main objective of this study was to determine the antioxidant and antibacterial activity of the extracts of the root barks of S. singueana. Methodology: The antibacterial activities of the extracts (determination of Minimal Inhibitory “MIC” and Minimal Bactericidal Concentration “MBC”) were determined by using agar well diffusion method. In addition to this the total flavonoid and total phenolic contents were determined by using aluminum chloride colorimetric complex assay and Folin-Ciocalteu method respectively. Results: Our results revealed that the total flavonoid content of the extracts is ranged from 30.39 mgQE/100 g to 240.83 mgQE/100 g. The extracts also showed good antioxidant activity and total phenolic content as well as weak to moderate antibacterial activity against some bacteria. Conclusions: The extracts of the root bark of Senna sinueana showed good total flavonoid content, DPPH radical scavenging activity and antibacterial activity. In addition to this, the extracts also showed the presence of some important compounds by phytochemical analysis.

scholarly journals Phytochemical Analysis, Antibacterial Activity, FTIR and GCMS Analysis of Ceropegia juncea Roxb.

2018 ◽  
Vol 9 (07) ◽  
Author(s):  
Visveshwari M. ◽  
Subbaiyan B. ◽  
Thangapandian V.
Keyword(s):  
Antibacterial Activity ◽  
Functional Group ◽  
Methanol Extract ◽  
Biological Activities ◽  
Phytochemical Analysis ◽  
Ftir Analysis ◽  
Major Peak ◽  
Zone Of Inhibition ◽  
Natural Health ◽  
Aerial Parts

The present study was focused on the preliminary phytochemical, antibacterial activity, FTIR and GC-MS analysis of aerial parts of C. juncea. Phytochemical analysis of three extracts showed that the presence of alkaloids, tannins and flavonoids. The methanol extract of aerial parts were found to be exhibit highest zone of inhibition against S. aureus (19.3mm), P. florescence (17.6mm) and K. pneumonia (17.0mm). FTIR analysis of the methanol extract given the major peak observed was at wavenumber 3354.08cm-1 that indicates the presence of O-H Alcohol functional group. 29 components were identified through the GCMS analysis of methanol extract. From results to confirm the fact that C. juncea possesses potential of bioactive compounds which are responsible for the biological activities that is useful for natural health.

The Antioxidant Activity and Phytochemical Analysis of Medicinal Plant Veronica Biloba

2020 ◽  
Vol 5 (1) ◽  
Author(s):  
AMIR HASSAN ◽  
Himayat Ullah ◽  
Muhammad Israr
Keyword(s):  
Antioxidant Activity ◽  
Medicinal Plant ◽  
Ethyl Acetate ◽  
Biological Activities ◽  
Continuous Process ◽  
Natural Antioxidants ◽  
Positive Control ◽  
Phytochemical Analysis ◽  
Uniform Size ◽  
Health Related

A medicinal plant veronica genus has 450 well known species and found across both temperate and hemisphere region their 26 species are endemic and known in a total of 79 popular species and are widely utilized throughout the world due to important biological activities. In this study fully powdered uniform size specie veronica biloba plant taken in porous bag were manually subjected to soxhlet hot continuous process for cyclization of extraction using ethanol (300 mL) a concentrated dried extract obtained after solvent evaporation. Furthermore, liquid-liquid extracted fractions as water, dichloromethane, n-hexane, and ethyl acetate yields results founds polar fraction with highest percentage (water 47.51 %). The phytochemical screening of veronica biloba has shown all major compounds entirely present in extracts. One of the primitive phenolic compound flavonoid is present in plant and show potency towards antioxidants. All the extracted fractions of plant showed excellent antioxidant activity using a stable DPPH (1,1-diphenyl-2-picryl-hydrazyl) method at concentration (range from 31.25 to 500µg/mL). Primary our ethyl acetate extract fraction showed highest inhibition potential at IC50 = 1.70±0.05µg/mL which is much closer to a standard positive control Propyl gallate showed IC50 = 1.6±0.05µg/mL percent potential. The purification and isolation of these extract is important which can provide us help in novel antioxidants discovery also natural antioxidants currently in cosmetics products, food and therapeutics health related products significantly demanded because they are very effective, efficient and harmless as compared to synthetic one.

scholarly journals Phytochemical composition and biological investigation of Trichilia emetica Vahl. seed extracts

2020 ◽  
Vol 9 (2) ◽  
pp. 1111-1116
Keyword(s):  
Antifungal Activity ◽  
Ethyl Acetate ◽  
Fungal Pathogens ◽  
Methanol Extract ◽  
Biological Activities ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Phytochemical Analysis ◽  
Biological Investigation ◽  
Seed Extracts

The current study investigated the phytochemical analysis, chemical composition, antioxidant, cytotoxic and antifungal activity of Trichilia emetica seed extracts. Preliminary phytochemical screening and quantification were realized by GC-MS. Consecutive extractions (methanol, ethanol, ethyl acetate, hexane, chloroform and distilled water) were carried out and phytochemical tests performed to represent the variety of phytochemicals. Seed extracts variably displayed alkaloids, cardiac glycosides, phenols, sterols, terpenoids and flavonoids but there was no indication of saponins and tannins in all extracts. The biological investigation of the methanolic extract showed significant biological activities. The results obtained revealed that methanol extract has presented significant IC50 =5.94 µg/mL radical scavenging activity. Minimum inhibitory concentration (MIC) of hexane and chloroform fractions displayed antifungal activity (40.95-100 and 76.27-100.11, respectively) against all three fungal pathogens, whilst the methanol extract inhibited C. krusei and C. parapsilosis and the ethyl acetate inhibited C. parapsilosis only. The results obtained revealed the medicinal importance of the plant and methanol extract of T. emetica would aid researchers to exploit as potential antifungal and antioxidant agent.

scholarly journals Biological activities and phytochemical analysis of extracts Afrostyrax lepidophyllus Mildbr. seeds

2017 ◽  
Vol 6 (2) ◽  
pp. 102-106
Author(s):  
Namkona Armel Frédéric ◽  
◽  
Bolevane Ouantinam Serge Florent ◽  
Moustapha Fatia ◽  
Worowounga Xavier ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Ethyl Acetate ◽  
Dry Matter ◽  
Inhibitory Concentration ◽  
Biological Activities ◽  
Phytochemical Analysis ◽  
Tannin Content ◽  
Weak Correlation ◽  
Polyphenol Content ◽  
Human Diet

Oxidative stress, one of the causes of certain diseases, prompts the search for new antioxidant molecules to reduce or limit the damage caused by it. In this study, the antioxidant, anti-inflammatory and anti-xanthine oxidase activity of the extracts (cyclohexane, dichloromethane, ethyl acetate and methanol) of the Afrostyrax lepidophillus seeds used in the human diet and the Central African Pharmacopoeia Been measured. Total pholyphenols, flavonoids, tannins and anthocyanins were also quantified in this work .The IC50 (inhibitory concentration which reduces free radicals by 50%) of the antioxidant activity of their extracts are respectively of the order of 3890,5±4,3, 3313,8±3,5, 1872,8±1,0 et 248,4±0,1 mg/L. The phytochemical analysis of the various extracts (cyclohexane, dichloromethane, ethyl acetate and methanol) made it possible to quantify the polyphenol content of 13,1±0,4, 30,5±0,5, 51,8±1,6 et 33,2±4,0 mg eq AG/g Dry matter (MS). The tannin content is of the order of 2,35±0,3, 10,68±0,1, 7,78±0,2, mg eq Cat/g MS, That of anthocyanins is 0.79 ± 0.04, 0.65 ± 0.02, 1.65 ± 0.07 and 0.18 ± 0.03 mg eq C3GE / g MS. The analysis of the different correlations between the chemical families and the antioxidant activity showed that there is a weak correlation between the biological activity and the phenolic compounds.

scholarly journals BIOLOGICAL ACTIVITIES OF GARCINIA MANGOSTANA

2017 ◽  
Vol 10 (9) ◽  
pp. 272 ◽  
Author(s):  
Jebalsy Lalitha ◽  
Prince Clarance P ◽  
Jerin Sales T ◽  
Mary Archana A ◽  
Agastian P
Keyword(s):  
Mass Spectrometry ◽  
Gas Chromatography ◽  
Ethyl Acetate ◽  
Antioxidant Activities ◽  
Biological Activities ◽  
Gas Chromatography Mass Spectrometry ◽  
Antioxidant Compounds ◽  
Phytochemical Analysis ◽  
Garcinia Mangostana

  Objective: The aim of the present study was to investigate in vitro antibacterial, antifungal, and antioxidant activities of different solvent extracts of Garcinia mangostana leaves.Methods: The powdered leaf was subjected to sequential extraction using hexane, ethyl acetate (EA), and methanol. The extracts were subjected to quantitative and qualitative phytochemical analysis, antimicrobial, and antioxidant activities. The best solvent extract was subjected to gas chromatography–mass spectrometry (GC-MS) analysis.Results: The highest activity was recorded in EA extract which was subjected to GC-MS analysis revealing the presence of squalene (17.09%).Conclusion: From this present study, we conclude that EA is the best solvent for extracting antimicrobial and antioxidant compounds from the leaves of G. mangostana.

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