Effects of Eight Weeks of Resistance Exercises on Expression of Neurotrophins and Trk Receptors in Alzheimer Model Male Wistar Rats

This study aims to evaluate the effects of eight weeks of resistance exercises on expression of neurotrophins and Trk receptors in Alzheimer model male Wistar rats. For this purpose, 32 mature male Wistar rats with mean weights of 230 to 280 g were chosen and divided into Alzheimer and Sham groups. Rats in the Sham group received normal saline while rats in the Alzheimer group received STZ via intraventricular injection. These rats were then divided into the following four subgroups: 1. Resting Sham, 2. Exercising Sham, 3. Resting Alzheimer, and 4. Exercising Alzheimer. The two exercising rat subgroups, exercised three times a week for a period of eight weeks. A weight was attached to their tails and they had to carry this weight on a 26-step ladder in each cycle. Resting groups were handled every day to minimize the effects of stress level. At the end of the 8th week and 24 hours after the last exercise session (to avoid the effects of the last exercise session), the rats were put under deep anesthesia and scarified by head separation. Hippocampus tissues were precisely extracted and samples were sent to laboratory for molecular and cellular tests. In order to investigate gene expression, Quantitative RT-PCR was used. Results of the tests for comparing the means of BDNF, NT3, NGF, TrkA, and TrkB in two rat groups showed that with error levels of less than 5% and confidence of over 95%, there is a significant difference in the amounts of BDNF, NT3, NGF, TrkA, and TrkB between exercising rats and resting rats. These amounts were much higher in exercising Alzheimer rats group. Eight weeks of resistance exercises increased the expression of BDNF, NT3, and NGF genes as well as TrkA and TrkB receptors in Alzheimer model Wistar rats.

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Pengaruh Profilaksis Ekstrak Ubi Jalar Ungu (Ipomoea batatas L.) terhadap Kadar Malondialdehida Hepar Tikus Putih (Rattus norvegicus) Jantan Galur Wistar yang Diinduksi Karagenan

Hang Tuah Medical journal ◽

10.30649/htmj.v15i2.66 ◽

2018 ◽

Vol 15 (2) ◽

pp. 146

Author(s):

BRILIAN DINANTI ◽

FITRI HANDAJANI

Keyword(s):

Sweet Potato ◽

Ipomoea Batatas ◽

Wistar Rats ◽

Group Method ◽

Control Group ◽

Intraplantar Injection ◽

Significant Difference ◽

Male Wistar Rats ◽

Potato Extract ◽

Purple Sweet Potato

Liver is an organ with complex metabolism. When the liver is inflamed, cellular immunity will defend against inflammatory agents by stimulating immune cells to produce reactive oxygen species (ROS). Excessive ROS accumulation cause oxydative stress with increased liver malondialdehyde (MDA) level. Some researches showed that purple sweet potato contain flavonoids (anthocyanins) that functioned as antioxydants. This study aimed to show the prophylactic effect of purple sweet potato extract to the liver MDA level of male Wistar rats induced by carrageenan.This study used post-only control group method using 18 male Wistar rats divided into 3 groups: group of rats without treatment, group of rats induced by 0,1 ml of 1% carrageenan by intraplantar injection on day-8, and group of rats given with 872 mg/kgBW of purple sweet potato extract for 7 days and induced by 0,1 ml of 1% carrageenan. In the end of the study, the liver MDA levels were measured by Thio-Barbituric Acid method on each groups.The results of One-Way ANOVA test showed there was no significant difference (p = 0,290) between group of rats without treatment (x̅= 207,50) and group of rats induced by carrageenan (x̅=233,17). Then, there is no significant difference (p = 0.978) between group of rats induced by carrageenan and group of rats given with prophylactic purple sweet potato extract and induced by carrageenan (x̅= 232,50).The conclusion of this study is giving intraplantar injection of carrageenan can increase liver MDA level insignificantly and giving prophylactic purple sweet potato extract has an effect to decrease the liver MDA level of rats induced by carragenan insignificantly because it contains anthocyanins as antioxidants. Keywords: Liver, Ipomoea batatas L., Malondialdehyde, Anthocyanins

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Potential Antiseptic of Rhodomyrtus tomentosa leaves extract on Healing wound in Male Wistar rats

Research Journal of Pharmacy and Technology ◽

10.52711/0974-360x.2021.00548 ◽

2021 ◽

pp. 3143-3149

Author(s):

Endang Sri Purwanti Ningsih ◽

Noorlaila Noorlaila ◽

Ikhwan Rizki Muhammad ◽

Windy Yuliana Budianto

Keyword(s):

Wound Healing ◽

Wistar Rats ◽

Leaf Extract ◽

Wound Care ◽

Positive Control ◽

Negative Control ◽

Control Group ◽

Significant Difference ◽

Male Wistar Rats ◽

Rhodomyrtus Tomentosa

Background: The process of wound healing is influenced by various factors such as age, hormones, and wound care. Wound care is done to accelerate wound healing which can be done by various methods, one of them is traditional care. Traditional wound care can use medicinal plants. Rhodomyrtus tomentosa is a medicinal plant that has an antioxidant, anti-inflammatory, antitumor and antibacterial content. Thus this study aims to evaluate the effectiveness of the antiseptic solution of the Rodhomyrtus tomentosa leaf extract on wound healing in male Wistar rats. Method: this research is pure experimental research with post test only control group design. Thirty male white rats were divided into five groups, namely negative control, positive control, Rhodomyrtus tomentosa leaf extract 15%, 30%, and 60%. Rhodomyrtus tomentosa leaf extraction was carried out by maceration method with 70% ethano solvent. The extraction results are divided into 3 concentrations (15%, 30% and 60%). The wound healing process was evaluated by measuring the length of the wound manually from 0 to 10 days in each group. Meanwhile, the number of fibroblast cells was calculated through hematoxylin eosin (HE) staining and observed using an Olympus CX41 microscope with a 10x magnification and objective lens magnification in 3 fields. Result: There was a significant difference in the reduction in wound length (p =< 0,000) between the five experimental groups (Rhodomyrtus tomentosa leaf extract solution 15%, 30% and 60%, negative control and positive control. Solution of rhodomyrtus tomentosa leaf extract accelerated the increase in the number of fibroblasts compared to the negative control group (p = 0.003), but did not make a difference (p = 0.403) with the positive control group. Rhodomyrtus tomentosa leaf extraction solution had the same microscopic effect on the number of fibroblasts with a positive control group given 0.9% NaCl solution. Conclusion: There was a significant difference in the number of fibroblasts between all groups, but no difference in wound healing length.

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PENGARUH PEMBERIAN GEL KOMBINASI EKSTRAK GETAH PEPAYA (Carica papaya L) DAN EKSTRAK DAGING LIDAH BUAYA (Aloe vera) TERHADAP PROSES PENYEMBUHAN ULKUS TRAUMATIKUS PADA MALE WISTAR RATS YANG MENDERITA DIABETES MELITUS

ODONTO Dental Journal ◽

10.30659/odj.2.2.41-46 ◽

2015 ◽

Vol 2 (1) ◽

pp. 41

Author(s):

Umi Kulsum ◽

Ratnawati Hendari ◽

Siti Chumaeroh

Keyword(s):

Wound Healing ◽

Ulcer Healing ◽

Wistar Rats ◽

Povidone Iodine ◽

Healing Process ◽

Aloe Vera ◽

Control Group ◽

Significant Difference ◽

Male Wistar Rats ◽

Papaya Latex

Introduction: Research on the use of papaya and aloevera sap on wound healing each been done, but effects the combination of the two extracts on wound healing in diabetes mellitus condition has not been done.Purpose: This study aims to determine the effect of a gel combination of papaya latex and aloevera flesh extracts on the traumatics ulcer healing process in diabetic rats.Methods: True experimental laboratory research with the post-test-only control group design that performed on 28 male wistar rats which were divided intofour groups: DM and non-DM rat that treatment with povidone iodine and gel combination of papaya latex and aloevera flesh extract. The treatment was done for 7 days with smearing that was given 2x/day. Traumatics ulcer healing seen from the number of fibroblasts that then were analyzed by One WayAnova and post hoc LSD test.Results: The results showed the number of fibroblasts in DM and non-DM rat with povidone iodine were 44.6 and 42.73; in the DM and non-DM rat with a gel combination of papaya latex and aloe vera flesh extracts were 61.10 and 77.03. One Way Anova test resulted p-value = 0.000, it was mean that there were at least a significant difference on the number of fibroblasts in the two groups. Differences of the number fibroblasts was shown in all groups, except in the group DM and non-DM rats with povidone iodine (p = 0.764).Conclusion: Conclusion of the study was the provision of a gel combination of papaya latex and aloevera flesh extracts effect on the traumatics ulcer healing process in diabetic rats.

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PENGARUH EKSTRAK DAGING LIDAH BUAYA (Aloe Vera) TERHADAP PENYEMBUHAN ULSERASI MUKOSA MULUT PADA MALE WISTAR RATS

ODONTO Dental Journal ◽

10.30659/odj.1.1.25-28 ◽

2015 ◽

Vol 1 (1) ◽

pp. 25

Author(s):

Laila Fitrotuz Zahroh ◽

Rahmawati Sri Praptiningsih ◽

Moh. Baehaqi

Keyword(s):

Oral Mucosa ◽

Wistar Rats ◽

Healing Process ◽

Research Result ◽

Aloe Vera ◽

Control Group ◽

Control Group Design ◽

Significant Difference ◽

Male Wistar Rats

Background: Oral mucosa ulceration which often occurs usually in the form of white-yellowish spot with concave surface, reddish edge and pain. Based on previous research, Aloe vera process anti-inflammation substance that could help quickening ulceration healing process. This research aims to know the effect of Aloe vera flesh extract on Male wistar rats oral mucosa ulceration in-vivo. Method: this research was quasi experimental research with the post-test only control group design using Male wistar rats as the testing animal. In the research, there were three treatment groups: The first groups which was given aquadest treatment, second groups with Aloe vera flesh extract, and third groups which was given chlorhexidine gluconate 0,2% treatment. The data collecting was based on histopathology observation concerning the increase of fibroblast quantity. Result: The research result based on comparison test among the three groups with One Way Anova showed that on Day 3th, the average quantity of fibroblast didn't have significant difference between the treatment group and control group positive that was p>0,05, meanwhile on Day 7th every group showed significant difference p<0,05. Conclusion: It concluded that Aloe vera flesh extract has influence on the healing of Male wistar rats oral mucosa ulceration as shown by fibroblast increasing quantity.

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Chemoprevention by celecoxib in reflux-induced gastric adenocarcinoma in Wistar rats that underwent gastrojejunostomy

Acta Cirurgica Brasileira ◽

10.1590/s0102-86502009000300005 ◽

2009 ◽

Vol 24 (3) ◽

pp. 189-194 ◽

Cited By ~ 7

Author(s):

Frederico Theobaldo Ramos Rocha ◽

Laercio Gomes Lourenço ◽

Mário Jorge Jucá ◽

Valéria Costa ◽

Antenor Teixeira Leal

Keyword(s):

Gastric Adenocarcinoma ◽

Wistar Rats ◽

Exploratory Laparotomy ◽

Gastric Body ◽

Gastrojejunal Anastomosis ◽

Significant Difference ◽

Male Wistar Rats ◽

Laparotomy Group ◽

Group 2 ◽

Group 1

PURPOSE: To evaluate chemoprevention by celecoxib in cases of reflux-induced gastric adenocarcinoma, in Wistar rats that underwent gastrojejunostomy. METHODS: Sixty male Wistar rats of average age three months underwent surgery and were distributed into three groups: group 1, exploratory laparotomy; group 2, gastrojejunostomy; and group 3, gastrojejunostomy and daily celecoxib administration. After 53 weeks, the animals were sacrificed. Changes in the mucosa of the gastric body of group 1 and in the gastrojejunal anastomosis of groups 2 and 3, observed in histopathological and immunohistochemical examinations, were compared. All statistical analyses were performed using Epi-Info®, version 3.4.3. RESULTS: Comparison between groups 2 and 3 relative to the presence of adenocarcinoma showed a statistically significant difference (p=0.0023). Analysis of the association between groups 2 and 3 relative to COX-2 expression also showed a statistically significant difference (p=0.0018). CONCLUSION: Celecoxib had an inhibiting effect on gastric carcinogenesis induced by enterogastric reflux in an animal model.

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Effect of rifampicin on the biosynthesis of testosterone in rat testis

Acta Endocrinologica ◽

10.1530/acta.0.0970573 ◽

1981 ◽

Vol 97 (4) ◽

pp. 573-576 ◽

Cited By ~ 4

Author(s):

Lieselotte Nocke-Finck ◽

H. Breuer

Keyword(s):

Wistar Rats ◽

Microsomal Fraction ◽

Hydroxysteroid Dehydrogenase ◽

Tris Buffer ◽

Metabolic Pattern ◽

Rat Testis ◽

Significant Difference ◽

Male Wistar Rats ◽

17 Hydroxyprogesterone ◽

Generating System

Abstract. Male Wistar rats were treated with rifampicin (10 mg/kg) daily for 5 days. After 6 days, the rats were killed and the testes homogenised in tris buffer. The microsomal fractions were incubated with [4-14C]4-progesterone, [4-14C]17-hydroxyprogesterone or [4-14C]-androstene-3,17-dione, respectively, and a NADPH generating system. In rifampicin-treated animals, less [4-14C]progesterone was recovered after incubation with testicular microsomes than in controls. There was no significant difference in the formation of 17-hydroxyprogesterone between rifampicin-treated animals and the controls; however, a significant increase was found in the androstenedione fraction (+ 83%) and the testosterone fraction (+ 48%) under the influence of rifampicin. When [4-14C]17-hydroxyprogesterone or [4-14C]4-androstene-3,17-dione were incubated, no differences in the metabolic pattern were observed between treated and untreated rats. These findings suggest that rifampicin stimulates the activity of 17α-hydroxylase in the microsomal fraction of rat testes, thus leading to an increased formation of testosterone and androstenedione; in contrast, the 17,20-lyase and 17β-hydroxysteroid-dehydrogenase remain unaffected by rifampicin.

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Effect of Kefir, Yacon (Smallanthus sonchifolius) and the Association Between Yacon and Kefir on Intestinal Physiology in Rats With Colon Cancer

Current Developments in Nutrition ◽

10.1093/cdn/nzab037_019 ◽

2021 ◽

Vol 5 (Supplement_2) ◽

pp. 309-309

Author(s):

Neuza Costa ◽

Keila Zanardi ◽

Caroline Woelffel ◽

Andre Costa ◽

Mirelle Viana ◽

...

Keyword(s):

Colon Cancer ◽

Intestinal Permeability ◽

Wistar Rats ◽

Short Chain Fatty Acids ◽

Diet Group ◽

Standard Diet ◽

Smallanthus Sonchifolius ◽

Intestinal Physiology ◽

Significant Difference ◽

Male Wistar Rats

Abstract Objectives To evaluate the effect of consumption of yacon flour, kefir and the association between them on colon cancer induced by 1,2-dimethylhydrazine (DMH) in male Wistar rats, SCFA production, fecal pH and intestinal permeability. Methods The study was conducted with 60 adult Wistar rats divided into 5 groups. For 5 weeks, groups T, Y, K and YK received 1,2-dimethylhydrazine (DMH) to induce colon cancer. After 5 weeks of DMH administration, animals in groups C and T received the standard diet, group Y received a diet with yacon flour with 5% FOS, group K received 1mL /day of kefir and the YK group received a diet with yacon and kefir, for 15 weeks. After euthanasia, intestinal lesions, intraluminal pH, short-chain fatty acids (SCFA) and intestinal permeability were analyzed. Results An increase in macroscopic lesions was observed in groups K (58%) and YK (42%) and a reduction of 5% in group Y, compared to group T. In addition, an increase in neoplastic changes was observed in all groups compared to group T: Y (33%), K (67%) and YK (78%). There was no significant difference in the concentrations of acetate and proprionate, pH, lactulose and mannitol between groups, and butyrate was not found in the samples. Conclusions The consumption of yacon flour, kefir and their association did not influence intestinal physiology and promoted the worsening of the development of colon carcinogenesis in rats. Funding Sources FAPES - Fundação de Amparo à Pesquisa e Inovação do Espírito Santo CNPq - Conselho Nacional de Desenvolvimento Científico e Tecnológico

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Infiltrasi Neutrofil pada Tikus dengan Periodontitis setelah Pemberian Ekstrak Etanolik Kulit Manggis

Majalah Kedokteran Gigi Indonesia ◽

10.22146/majkedgiind.8520 ◽

2014 ◽

Vol 21 (1) ◽

pp. 33

Author(s):

Rendra Chriestedy Prasetya ◽

Nunuk Purwanti ◽

Tetiana Haniastuti

Keyword(s):

Inflammatory Disease ◽

Wistar Rats ◽

Ethanolic Extract ◽

Inflammatory Cells ◽

Neutrophil Infiltration ◽

Chronic Inflammatory Disease ◽

Periodontopathic Bacteria ◽

Significant Difference ◽

Male Wistar Rats ◽

Hematoxylin Eosin

Periodontitis adalah inflamasi kronis yang disebabkan oleh bakteri periodontopatogen. Pada periodontitis terjadi peningkatan infiltrasi neutrofil yang berfungsi untuk memfagositosis bakteri yang menginfiltrasi jaringan gingiva. Kulit manggis mempunyai bahan aktif yaitu xanton yang mempunyai efek antiinflamasi dengan jalan menghambat sintesis PGE2 sehingga akan menurunkan infiltrasi sel inflamasi. Penelitian ini bertujuan untuk mengetahui infiltrasi sel neutrofil pada periodontitis setelah pemberian ekstrak etanolik kulit manggis. Tikus wistar jantan sebanyak empat puluh delapan ekor diinduksi periodontitis dengan ligasi pada daerah subgingiva gigi anterior rahang bawah selama 7 hari. Setelah hari ke-7, ligasi dilepas selanjutnya tikus dibagi menjadi 4 kelompok perlakuan yaitu ekstrak kulit manggis 30 mg/kg BB dan 60 mg/kg BB, ibuprofen dan saline. Tikus dikorbankan pada hari ke-1, 3, 5 dan 7 setelah perlakuan. Jaringan pada bagian anterior rahang bawah ditanam dalam paraffin dan dilakukan pemotongan serial kemudian diwarnai dengan hematoxylin eosin. Jumlah neutrofil dihitung di bawah mikroskop dengan perbesaran 400x. Data jumlah neutrofil dianalisa dengan Two Way Anova. Hasil Two Way Anova menunjukkan perbedaan yang bermakna rerata sel neutrofil antar kelompok perlakuan (p<0,05) mengindikasikan pemberian ekstrak etanolik kulit manggis berpengaruh terhadap jumlah sel neutrofil. Dapat disimpulkan bahwa pemberian ekstrak etanolik kulit manggis mampu menurunkan infiltrasi sel neutrofil pada tikus yang diinduksi periodontitis.Neutrophil infiltration in rats with periodontitis after the granting of Ethanolic Extract Skin Mangosteen. Periodontitis is a chronic inflammatory disease caused by periodontopathic bacteria. When periodontitis occurs are followed by neutrophil infiltration. Mangosteen rind contains xanthone, an anti-inflammatory substance which inhibits the synthesis of PGE2 and therefore reduces inflammatory cells infiltration. This research aimed to study neutrophil cells infiltration in experimental-induced periodontitis rats after mangosteen rind ethanolic extract administration. Forty-eight male wistar rats were induced the periodontitis by putting silk ligature subgingivally around the neck of the anterior lower teeth for seven days. After the ligation had been taken out, the rats were divided into four groups and treated orally with mangosteen rind extract 60 mg/kg BB, 30 mg/kg BB, ibuprofen, and saline. The rats were sacrificed on the 1st, 3rd, 4th, seventh day after the treatment. Their anterior lower jaws were processed for paraffin embedded tissue, cut serially and stained with hematoxylin-eosin. The neutrophil cells were observed and counted under the microscope (400x). The data were analyzed using Anova. Anova result showed a significant difference among group (p<0,05) indicating that mangosteen rind ethanolic extract affected the number of neutrophils. In conclusion, mangosteen rind ethanolic extract reduced the number of neutrophil infiltration in periodontitis rats.

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The Effectiveness Fig Leaves (FICUS CARICA L) Steeping Water Towards SGOT SGPT Serum on Male Wistar Rats in Critical Hepatitis Model

Abstract Proceedings International Scholars Conference ◽

10.35974/isc.v7i1.2087 ◽

2019 ◽

Vol 7 (1) ◽

pp. 826-835

Author(s):

Yosi Julianti ◽

Untung Sudharmono

Keyword(s):

Treatment Group ◽

Wistar Rats ◽

Ficus Carica ◽

Negative Group ◽

Positive Group ◽

Treatment Groups ◽

Significant Difference ◽

Male Wistar Rats ◽

Food And Drink ◽

One Way Anova

Critical Hepatitisis is the inflammation of the liver that is caused by viruses, alchol and drugs which are characterized by the increasing values of SGOT SGPT. The purpose of this study is to determine the effectiveness of fig leaves (Ficuss carica L) steeping water towards the decrease of SGOT SGPT serum on male wistar rats in critical hepatitis model. The object of this study is 30 male wistar rats with the age of 2-3 months and weight 180-200 grams and randomly divide into 3 groups, which are the negative group that is only by giving an usual food and drink, the positive group and the treatment groups are given paracetamol 120mg/ oral for 7 days. The treatment group is given 0.65 gram/ day of fig leaves steeping water for 7 days. One way ANOVA of SPSS version 24 is used to analyze this research. The result of the study shows that there is a significant difference in SGOT serum level between the treatment group, positive group and negative group (p<0,05). And there is a significant difference in SGPT level between the treatment group and the positive group (p<0,05). The conclusion of this study is tin leaves (Ficus carica L) steeping water has an effect towards the decrease of SGOT SGPT in male Wistar rats in critical hepatitis model.

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Bioefficiency of Chromolaena odorata (Linn.) on hematological and lipid profiles in sublethal cyanide poisoning in male wistar rats

Journal of Applied and Natural Science ◽

10.31018/jans.v12i1.2215 ◽

2020 ◽

Vol 12 (1) ◽

pp. 13-18

Author(s):

Adeola. Folashade Ehigie ◽

Gbadebo Emmanuel Adeleke ◽

Fiyinfoluwa. D. Ojeniyi ◽

Leonard Ona Ehigie

Keyword(s):

Lipid Profile ◽

Blood Cells ◽

Wistar Rats ◽

Hematological Parameters ◽

Chromolaena Odorata ◽

Sodium Thiosulphate ◽

Cyanide Group ◽

Significant Difference ◽

Male Wistar Rats ◽

The Impact

Chromolaena odorata is an invasive plant which is acclaimed to have cyanide remediation potential from contaminated sites. This examination means to decide the impact of ethanol concentrates of C. odorata (ECO), sodium thiosulphate and a mix of both on hematological parameters and blood lipid profile of rodents presented to potassium cyanide. (KCN). A sum of thirty five male Wistar rats partitioned into seven groups of five units were used. KCN Group rats were administered with KCN alone. Rats in 100ECO, 150ECO, 200ECO groups were administered with 100, 150 and 200 mg/kg body weight of ECO respectively. Rats under Na2sS2O3 and Na2S2O3+ECO groups were administered 200 mg/kg sodium thiosulphate and sodium thiosulphate with ECO at 200 mg/kg respectively. The trial was done in about a month. Toward the finish of the investigation, the packed cell volume (PCV), hemoglobin level (Hb), Red blood cells (RBC) and white blood cells (WBC) were resolved utilizing known biochemical methodology. The outcomes demonstrated a noteworthy increment (p ? 0.05) in PCV, Hb, RBC and WBC level of remedial groups when contrasted with the cyanide group. Total cholesterol (TC), and triglyceride (TG) were altogether lower while HDL-cholesterol was fundamentally expanded in all the treated groups when contrasted and the untreated group given cyanide alone. However, no significant difference in LDL-cholesterol was indicated in all therapeutic groups compared with the cyanide group. The study revealed that C. odorata at the tested doses was able to improve the hematological parameters and lipid profile in cyanide exposed rats.

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