scholarly journals Bioefficiency of Chromolaena odorata (Linn.) on hematological and lipid profiles in sublethal cyanide poisoning in male wistar rats

2020 ◽  
Vol 12 (1) ◽  
pp. 13-18
Author(s):  
Adeola. Folashade Ehigie ◽  
Gbadebo Emmanuel Adeleke ◽  
Fiyinfoluwa. D. Ojeniyi ◽  
Leonard Ona Ehigie
Keyword(s):  
Lipid Profile ◽  
Blood Cells ◽  
Wistar Rats ◽  
Hematological Parameters ◽  
Chromolaena Odorata ◽  
Sodium Thiosulphate ◽  
Cyanide Group ◽  
Significant Difference ◽  
Male Wistar Rats ◽  
The Impact

Chromolaena odorata is an invasive plant which is acclaimed to have cyanide remediation potential from contaminated sites. This examination means to decide the impact of ethanol concentrates of C. odorata (ECO), sodium thiosulphate and a mix of both on hematological parameters and blood lipid profile of rodents presented to potassium cyanide. (KCN). A sum of thirty five male Wistar rats partitioned into seven groups of five units were used. KCN Group rats were administered with KCN alone. Rats in 100ECO, 150ECO, 200ECO groups were administered with 100, 150 and 200 mg/kg body weight of ECO respectively. Rats under Na2sS2O3 and Na2S2O3+ECO groups were administered 200 mg/kg sodium thiosulphate and sodium thiosulphate with ECO at 200 mg/kg respectively. The trial was done in about a month. Toward the finish of the investigation, the packed cell volume (PCV), hemoglobin level (Hb), Red blood cells (RBC) and white blood cells (WBC) were resolved utilizing known biochemical methodology. The outcomes demonstrated a noteworthy increment (p ? 0.05) in PCV, Hb, RBC and WBC level of remedial groups when contrasted with the cyanide group. Total cholesterol (TC), and triglyceride (TG) were altogether lower while HDL-cholesterol was fundamentally expanded in all the treated groups when contrasted and the untreated group given cyanide alone. However, no significant difference in LDL-cholesterol was indicated in all therapeutic groups compared with the cyanide group. The study revealed that C. odorata at the tested doses was able to improve the hematological parameters and lipid profile in cyanide exposed rats.

scholarly journals Some hematological parameters of Wistar rats treated with Chromolaena odorata leave extracts

2017 ◽  
Vol 90 (1) ◽  
Author(s):  
Henshaw Uchechi Okoroiwu ◽  
Ifeyinwa Maryann Okafor ◽  
Emmanuel Kufre Uko ◽  
Item Justin Atangwho
Keyword(s):  
Cell Volume ◽  
Wistar Rats ◽  
Hematological Parameters ◽  
Ethanol Extract ◽  
Control Group ◽  
Chromolaena Odorata ◽  
Mean Cell Volume ◽  
Significant Difference ◽  
The Mean ◽  
Ethanol Extracts

This study was designed to investigate the effects of the different extracts of Chromolaena odorata leave on the hematopoietic system of Wistar rats. Solvent extraction was used for the ethanol and aqueous extractions while decoction method was used for the crude extraction. Fifty Wistar rats of both sexes weighing 140-180 g were used for this study. They were divided into ten groups each containing five rats. The animals were fed the extracts by oral gavage once daily for 21 days. Blood sample was collected via cardiac artery. Hematological parameters were analyzed using automation method. The ethanol extract gave the highest extract yield. The aqueous, ethanol and crude extraction had median lethal toxicity (LD50) of 2738.6 mg/kg, 1581.1 mg/kg and 224.7 mg/kg, respectively. Significant difference (P<0.05) in the total white blood cell count was observed in the 75 mg/kg ethanol and 300 mg/kg crude extracts when compared with control group. Significant difference (P<0.05) in the hemoglobin concentration was observed in the 150 mg/kg ethanol extracts when compared with the control group. Significant difference (P<0.05) in the packed cell volume was seen in the 75 mg/kg aqueous, 150 mg/kg aqueous and 75 mg/kg ethanol extracts in respect to the control group. The mean cell volume, the mean platelet volume and platelet large cell ratio of the 75 mg/kg aqueous extract were significantly different (P<0.05) when compared with the control group. The present study showed possible treatment-induced hematopoietic function of C. odorata leave extracts.

scholarly journals A Comparative study on Efficacy of Lutein and Atorvastatin on Lipid Profile and Lipoprotein(A) in Hypercholesterolemic male Wistar Rats

2021 ◽  
Vol 14 (01) ◽  
pp. 503-511
Author(s):  
Soundarya Priyadharsini K ◽  
Mali Kalpana Ramanna ◽  
Somu L ◽  
Krishna Prasad T
Keyword(s):  
Body Weight ◽  
Lipid Profile ◽  
Wistar Rats ◽  
Treated Group ◽  
Diet Group ◽  
Lipoprotein A ◽  
Group I ◽  
Significant Difference ◽  
Male Wistar Rats ◽  
Progression Of Atherosclerosis

Background Hypercholesterolemia is the predominantfactor in developing atherosclerosis and myocardial diseases.A major contributor for the progression of atherosclerosis is abnormalities in lipid and lipoprotein metabolism.Hence the objectives of the study was to estimatethe comparative efficacy of Lutein with atorvastatin on lipid profile and lipoprotein(a) and to estimate the histopahthological changes in hypercholesterolemic male wistar rats. Materials and Methods Experimental Wistar rats (male) were grouped into six. Each group contains 6 rats. Group I is control. Group II received cholesterol rich diet. Group III received cholesterol rich diet and the drug Atorvastatin 5mg/kg. Group IV received cholesterol rich diet and the drugLutein 25mg/kg. Group V received cholesterol rich diet and the drugLutein 50mg/kg. Group VI received cholesterol rich diet and the drug Lutein100mg/kg. At the end of 16 weeks, Blood samples from each rats was taken through retro-orbital puncture to evaluate serum lipoproteinsand lipoprotein(a) and thenwistar rats were sacrificed underinjection I.M Ketamine,Aortaand Liverwere dissected out and sent for histopathological studies. Results The plasma LDL, VLDL, Triglycerides, total cholesterol, lipoprotein(a) levels were reduced in all lutein treated groups and atorvastatin treated group compared to high cholesterol diet group. A significant rise in HDL levels was noted in all Lutein treated groups and atorvastatin treated group. No statistically significant difference was seen between Atorvastatin 5mg/kg body weight and Lutein 100mg/kg body weight on reduction of total cholesterol.The efficacy of the drug Lutein in progression of atherosclerosis and its cytoprotective action in liver was proved in this study. Conclusion This study indicates that Lutein has effect onreducing plasma lipoproteins&the study had shown significant antiatherogenic effect.

scholarly journals Assessment of Some Biomarkers in Liver, Kidney and Stomach Following Pineapple Juice Administration

2016 ◽  
Vol 4 ◽  
pp. 27-35
Author(s):  
Tolulope O. Oyesola ◽  
Olusoji A. Oyesola ◽  
S.O. Ayodeji Odukoya ◽  
Stephen Taiye Adelodun
Keyword(s):  
Total Protein ◽  
Wistar Rats ◽  
Hematological Parameters ◽  
Young Male ◽  
High Density Lipoproteins ◽  
Control Group ◽  
Water Control ◽  
Pineapple Juice ◽  
Significant Difference ◽  
Male Wistar Rats

This study looked into the physiochemical assessment of kidney, liver and stomach following pineapple juice (PJ) administration in normal Wistar rats. Thirty-two young male Wistar rats weighing between 180 - 200 g were used for this study. The juice was administered through oral cannula for 30 days. The animals were grouped into four (A-D). Group A received 3 ml of distilled water (Control), Group B received 3 ml/kg PJ, Group C received 6 ml/kg PJ administered at 3 ml twice daily and Group D received 9 ml/kg PJ at 3 ml three times daily for one month. Blood samples were collected a day post-administration through cardiac puncture for hematological and biochemical studies. Mucosal tissues were scraped from the stomach and homogenized to check for total protein and some other gastric enzymes. The study showed a significant decrease (P < 0.05) in cholesterol and triglycerides (113.4 ± 1.60-104.4 ± 1.66 and 104.4 ± 1.66-74.0 ± 2.95) while low density and high density lipoproteins showed no significant difference. Electrolyte concentrations of sodium, potassium, chloride, bicarbonate, urea and creatinine showed significant decreases. Liver enzyme parameters were significantly increased (P > 0.05) for ALP (40.0 ± 4.05 -79.4 ± 6.61 IU/L), AST (115.2 ± 1.46-178.4 ± 2.00 IU/L), ALT (86.0 ± 2.68-72.2 ± 3.90 IU/L) and ALB (3.16 ± 0.09 - 3.84 ± 0.02 IU/L). Results of Total Protein showed a significant increase while bilirubin is insignificantly different compared with the control. Results for SOD, TBAR, CATAL and MDA showed no significant differences. The study revealed that the hematological parameters assayed: Hb (11.85 ± 2.0 - 14.12 ± 1.5 g/100 ml), RBC count (6.27 ± 0.1-9.92 ± 1.5×106/mm3) and Total WBC counts (2.35 ± 0.6 - 4.47 ± 0.5 × 103/mm3) were significantly higher (P < 0.05). We concluded that the oral administration of PJ to Wistar rats for one month is beneficial by reducing cholesterol level and some toxic gastric enzymes. It also increased hematopoietic outputs, and served as an immune booster judging from the Total WBC count results while its effect on liver function is minimal based on this present study

scholarly journals Effect of orchidectomized and exogenous administration of testosterone on the quantification of platelets in male wistar rats

2021 ◽  
Vol 21 (4) ◽  
pp. 727-735
Author(s):  
Milagros A. Fuentes Vargas ◽  
Lilian C. Lovón Caso ◽  
Marcia A. Paredes Salazar ◽  
Jheydi A. Cahuana Gutierrez ◽  
Ana M. Apaza Choquehuanca ◽  
...  
Keyword(s):  
Wistar Rats ◽  
Hormone Replacement ◽  
Study Groups ◽  
Physiological Solution ◽  
The Other ◽  
Anabolic Androgenic Steroids ◽  
Significant Difference ◽  
Male Wistar Rats ◽  
Androgenic Steroids ◽  
The Impact

Introduction: Anabolic-androgenic steroids (AAS) modify the physiological functioning of the cardiovascular system and have possible effects on the origin of cardiovascular thrombosis. Objective: To determine the impact of the testosterone metabolite on platelet quantification in ORX rats with or without DHT replacement. Material and methods: 24 male 45-day-old Wistar rats underwent orchidectomy with a simple scrotal incision. At 2.5 months of age, the 24 rats were divided into 4 study groups. Half of the 12 male ORX rats received hormone replacement with dihydrotestosterone propionate (DHT) at a 2 mg/kg dose via subcutaneous injection for 7 days and the other half received a physiological solution (0.9% NaCl). The same occurred in the 12 non-ORX males (SHAM). After 7 days of the administration, blood was collected by orbital puncture, and platelet quantification was performed. Results: A significant difference (ANOVA, p < 0.005) was found between the 4 groups. When performing the Dunn Method, a significant difference (p < 0.05) was found in the endogenous administration of DHT between Sham rats and rats ORX + 0.9% NaCl but not with ORX + DHT. Conclusions: DHT induces an increase in platelet quantification inrats Sham and not in ORX rats with DHT, which may affect the increase in platelet quantification.

Pengaruh Profilaksis Ekstrak Ubi Jalar Ungu (Ipomoea batatas L.) terhadap Kadar Malondialdehida Hepar Tikus Putih (Rattus norvegicus) Jantan Galur Wistar yang Diinduksi Karagenan

2018 ◽  
Vol 15 (2) ◽  
pp. 146
Author(s):  
BRILIAN DINANTI ◽  
FITRI HANDAJANI
Keyword(s):  
Sweet Potato ◽  
Ipomoea Batatas ◽  
Wistar Rats ◽  
Group Method ◽  
Control Group ◽  
Intraplantar Injection ◽  
Significant Difference ◽  
Male Wistar Rats ◽  
Potato Extract ◽  
Purple Sweet Potato

<p>Liver is an organ with complex metabolism. When the liver is inflamed, cellular immunity will defend against inflammatory agents by stimulating immune cells to produce reactive oxygen species (ROS). Excessive ROS accumulation cause oxydative stress with increased  liver malondialdehyde (MDA) level. Some researches showed that purple sweet potato contain flavonoids (anthocyanins) that functioned as antioxydants. This study aimed to show the prophylactic effect of purple sweet potato extract to the liver MDA level of male Wistar rats induced by carrageenan.</p><p>This study used post-only control group method using 18 male Wistar rats divided into 3 groups: group of rats without treatment, group of rats induced by 0,1 ml of 1% carrageenan by intraplantar injection on day-8, and group of rats given with 872 mg/kgBW of purple sweet potato extract for 7 days and induced by 0,1 ml of 1% carrageenan. In the end of the study, the liver MDA levels were measured by Thio-Barbituric Acid method on each groups.</p><p>The results of One-Way ANOVA test showed there was no significant difference (p = 0,290) between group of rats without treatment (<em>x̅</em>= 207,50) and group of rats induced by carrageenan (<em>x̅</em>=233,17). Then, there is no significant difference (p = 0.978) between group of rats induced by carrageenan and group of rats given with prophylactic purple sweet potato extract and induced by carrageenan (<em>x̅</em>= 232,50).</p><p>The conclusion of this study is giving intraplantar injection of carrageenan can increase liver MDA level insignificantly and giving prophylactic purple sweet potato extract has an effect to decrease the liver MDA level of rats induced by carragenan insignificantly because it contains anthocyanins as antioxidants.</p><p> </p><strong>Keywords: </strong>Liver, <em>Ipomoea batatas</em> L., Malondialdehyde, Anthocyanins

Hematological condition of breeding bulls after a long winter period of operation in the conditions of the Udmurt Republic

2022 ◽  
pp. 67-73
Author(s):  
A. Abilov ◽  
A. Azhmyakov ◽  
I. Novgorodova ◽  
N. Bogolyubova
Keyword(s):  
Red Blood Cells ◽  
Reference Values ◽  
Blood Cells ◽  
Hematological Parameters ◽  
White Blood Cells ◽  
Winter Period ◽  
Black And White ◽  
Significant Difference ◽  
White Breed ◽  
Udmurt Republic

Purpose: to study hematological parameters of blood in bulls-producers of dairy breeds on the day of semen collection in the Udmurt Republic after a long winter period of operation, depending on the breeds, age and place of selection.Materials and methods. The work was performed at the Federal Research Center for Animal Husbandry named after Academy Member L. K. Ernst on the basis of AO "Udmurtplem" of the Udmurt Republic in the period from 2020 to 2021 on dairy bulls (n=20) aged 15-69 months, including a purebred Holstein breed of domestic selection (n=6), a Holstein black-and-white breed of European selection (the Netherlands, n=6), a black-and-white breed with blood transfusion on Holsteins on at the level of 94-98% (n=8). The content of white blood cells, red blood cells, hemoglobin and hematocrit, depending on age and breed, was studied on the ABC VET hematological analyzer on the day of taking the seed.Results. It was found that on average, in 20 bulls aged 15-69 months, the level of white blood cells was at the level of reference values of 8.8±0.25 x 109/l, red blood cells 10.3 x 1012/l, which is 50% more than the reference values. The concentration of hemoglobin is 128.0±2.92 g/l, hematocrit is on average 54.3%, with a norm of 24-46%. Breeding bulls at a reliable level, differing in age, showed that some animals had high indicators for red blood cells of 10.3±0.26 x 102, for hematocrit of 54.3% against 24-46% of reference values. Also, according to hematological indicators, there was a tendency to increase the concentration of hemoglobin and hematocrit in European-bred bulls.Conclusion. The study of the variability in hematological parameters depending on the selection showed that there is no significant difference in leukocytes and all indicators are at the level of reference values, and in erythrocytes more than 50% than the highest indicators. The highest hemoglobin values were at the level of 141-156 g/l instead of 128 g/l according to the highest reference values. Hematocrit also showed high max values in all groups in comparison with the reference values of 59-66% versus 46% in the norm. It is necessary when analyzing hematological blood parameters in addition to the average statistical indicators (M+m) also, monitor the variability (min-max) in order to obtain more objective information.

Potential Antiseptic of Rhodomyrtus tomentosa leaves extract on Healing wound in Male Wistar rats

2021 ◽  
pp. 3143-3149
Author(s):  
Endang Sri Purwanti Ningsih ◽  
Noorlaila Noorlaila ◽  
Ikhwan Rizki Muhammad ◽  
Windy Yuliana Budianto
Keyword(s):  
Wound Healing ◽  
Wistar Rats ◽  
Leaf Extract ◽  
Wound Care ◽  
Positive Control ◽  
Negative Control ◽  
Control Group ◽  
Significant Difference ◽  
Male Wistar Rats ◽  
Rhodomyrtus Tomentosa

Background: The process of wound healing is influenced by various factors such as age, hormones, and wound care. Wound care is done to accelerate wound healing which can be done by various methods, one of them is traditional care. Traditional wound care can use medicinal plants. Rhodomyrtus tomentosa is a medicinal plant that has an antioxidant, anti-inflammatory, antitumor and antibacterial content. Thus this study aims to evaluate the effectiveness of the antiseptic solution of the Rodhomyrtus tomentosa leaf extract on wound healing in male Wistar rats. Method: this research is pure experimental research with post test only control group design. Thirty male white rats were divided into five groups, namely negative control, positive control, Rhodomyrtus tomentosa leaf extract 15%, 30%, and 60%. Rhodomyrtus tomentosa leaf extraction was carried out by maceration method with 70% ethano solvent. The extraction results are divided into 3 concentrations (15%, 30% and 60%). The wound healing process was evaluated by measuring the length of the wound manually from 0 to 10 days in each group. Meanwhile, the number of fibroblast cells was calculated through hematoxylin eosin (HE) staining and observed using an Olympus CX41 microscope with a 10x magnification and objective lens magnification in 3 fields. Result: There was a significant difference in the reduction in wound length (p =< 0,000) between the five experimental groups (Rhodomyrtus tomentosa leaf extract solution 15%, 30% and 60%, negative control and positive control. Solution of rhodomyrtus tomentosa leaf extract accelerated the increase in the number of fibroblasts compared to the negative control group (p = 0.003), but did not make a difference (p = 0.403) with the positive control group. Rhodomyrtus tomentosa leaf extraction solution had the same microscopic effect on the number of fibroblasts with a positive control group given 0.9% NaCl solution. Conclusion: There was a significant difference in the number of fibroblasts between all groups, but no difference in wound healing length.

scholarly journals PENGARUH PEMBERIAN GEL KOMBINASI EKSTRAK GETAH PEPAYA (Carica papaya L) DAN EKSTRAK DAGING LIDAH BUAYA (Aloe vera) TERHADAP PROSES PENYEMBUHAN ULKUS TRAUMATIKUS PADA MALE WISTAR RATS YANG MENDERITA DIABETES MELITUS

2015 ◽  
Vol 2 (1) ◽  
pp. 41
Author(s):  
Umi Kulsum ◽  
Ratnawati Hendari ◽  
Siti Chumaeroh
Keyword(s):  
Wound Healing ◽  
Ulcer Healing ◽  
Wistar Rats ◽  
Povidone Iodine ◽  
Healing Process ◽  
Aloe Vera ◽  
Control Group ◽  
Significant Difference ◽  
Male Wistar Rats ◽  
Papaya Latex

Introduction: Research on the use of papaya and aloevera sap on wound healing each been done, but effects the combination of the two extracts on wound healing in diabetes mellitus condition has not been done.Purpose: This study aims to determine the effect of a gel combination of papaya latex and aloevera flesh extracts on the traumatics ulcer healing process in diabetic rats.Methods: True experimental laboratory research with the post-test-only control group design that performed on 28 male wistar rats which were divided intofour groups: DM and non-DM rat that treatment with povidone iodine and gel combination of papaya latex and aloevera flesh extract. The treatment was done for 7 days with smearing that was given 2x/day. Traumatics ulcer healing seen from the number of fibroblasts that then were analyzed by One WayAnova and post hoc LSD test.Results: The results showed the number of fibroblasts in DM and non-DM rat with povidone iodine were 44.6 and 42.73; in the DM and non-DM rat with a gel combination of papaya latex and aloe vera flesh extracts were 61.10 and 77.03. One Way Anova test resulted p-value = 0.000, it was mean that there were at least a significant difference on the number of fibroblasts in the two groups. Differences of the number fibroblasts was shown in all groups, except in the group DM and non-DM rats with povidone iodine (p = 0.764).Conclusion: Conclusion of the study was the provision of a gel combination of papaya latex and aloevera flesh extracts effect on the traumatics ulcer healing process in diabetic rats.

scholarly journals PENGARUH EKSTRAK DAGING LIDAH BUAYA (Aloe Vera) TERHADAP PENYEMBUHAN ULSERASI MUKOSA MULUT PADA MALE WISTAR RATS

2015 ◽  
Vol 1 (1) ◽  
pp. 25
Author(s):  
Laila Fitrotuz Zahroh ◽  
Rahmawati Sri Praptiningsih ◽  
Moh. Baehaqi
Keyword(s):  
Oral Mucosa ◽  
Wistar Rats ◽  
Healing Process ◽  
Research Result ◽  
Aloe Vera ◽  
Control Group ◽  
Control Group Design ◽  
Significant Difference ◽  
Male Wistar Rats

Background: Oral mucosa ulceration which often occurs usually in the form of white-yellowish spot with concave surface, reddish edge and pain. Based on previous research, Aloe vera process anti-inflammation substance that could help quickening ulceration healing process. This research aims to know the effect of Aloe vera flesh extract on Male wistar rats oral mucosa ulceration in-vivo. Method: this research was quasi experimental research with the post-test only control group design using Male wistar rats as the testing animal. In the research, there were three treatment groups: The first groups which was given aquadest treatment, second groups with Aloe vera flesh extract, and third groups which was given chlorhexidine gluconate 0,2% treatment. The data collecting was based on histopathology observation concerning the increase of fibroblast quantity. Result: The research result based on comparison test among the three groups with One Way Anova showed that on Day 3th, the average quantity of fibroblast didn't have significant difference between the treatment group and control group positive that was p>0,05, meanwhile on Day 7th every group showed significant difference p<0,05. Conclusion: It concluded that Aloe vera flesh extract has influence on the healing of Male wistar rats oral mucosa ulceration as shown by fibroblast increasing quantity.

scholarly journals In-vivo Antiplasmodial Effect of Dihydroartemisinin-Piperaquine-Nitrofurantoin on Plasmodium berghei- Infected Mice

2021 ◽  
pp. 12-20
Author(s):  
Udeme O. Georgewill ◽  
Festus Azibanigha Joseph ◽  
Elias Adikwu
Keyword(s):  
Plasmodium Berghei ◽  
Blood Cells ◽  
Hematological Parameters ◽  
White Blood Cells ◽  
Positive Control ◽  
Negative Control ◽  
Antiplasmodial Activity ◽  
Significant Difference ◽  
Tract Infections

Nitrofurantoin (NT) used for the treatment of urinary tract infections may have antiplasmodial activity. Dihydroartemisinin-piperaquine (DP) is an artemisinin based combination therapy used for the treatment of malaria. This study evaluated the antiplasmodial effect of dihydroartemisinin-piperaquine-nitrofurantoin (DP-NT) on mice infected with Plasmodium berghei. Adult Swiss albino mice (30-35 g) of both sexes were used. The mice were randomly grouped, inoculated with Plasmodium berghei, and treated orally with DP (1.7/13.7 mg/kg), NT (57.1 mg/kg) and DP-NT (1.71/13.7/ 57.1 mg/kg), respectively using curative, prophylactic and suppressive tests. The negative control was orally treated with normal saline (0.3 mL), while the positive control was orally treated with chloroquine CQ (10mg/kg). After treatment, blood samples were collected and evaluated for percentage parasitemia, inhibitions and hematological parameters. Liver samples were evaluated for histological changes. The mice were observed for mean survival time (MST). Treatment with DP-NT decreased parasitemia levels when compared to individual doses of DP and NT with significant difference observed at p<0.05. DP-NT prolonged MST when compared to individual doses of DP and NT with significant difference observed at p<0.05. The decrease in packed cell volume, red blood cells, hemoglobin and increase in white blood cells in parasitized mice were significantly restored by DP-NT  when compared to individual doses of DP and NT with difference observed at p<0.05. DP-NT eradicated liver Plasmodium parasite.  NT remarkably increased the antiplasmodial activity of DP. DP-NT may be used for the treatment of malaria.

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