scholarly journals To the question of amino acids and whey consumption

Author(s):  
O. O. Korytko

The article summarizes, in a concise form, information about the importance of amino acids in the context of solving the problem of fodder protein deficiency. Amino acids, as structural components of proteins, are central to the exchange of nitrogen-containing compounds and are vital for life processes. Essential amino acids are not synthesized by the body of animals and humans, but are prepared as a part of diets. Vegetable proteins are less complete than animal proteins due to the absence of some essential amino acids. Therefore, in feeding, plant feeds are balanced by limiting amino acids, which are obtained by chemical or microbiological synthesis, or combine protein sources, taking into account their amino acid composition. Amino acids are the primary microbial metabolites that are synthesized by microorganisms in the process of life. Due to the large-scale cultivation of microorganisms in industrial conditions, amino acids, proteins, preparations for increasing the productivity of crops and animals are receive. Appropriate types of microorganisms use ammonia oxidation energy to synthesize their own organic matter. Different bacteria for the synthesis of amino acids also use nitrogen, nitrates, urea. The microbiological synthesis and accumulation of metabolites in the substrate depends on the component composition, temperature. Microbial growth is usually limited by the nutrient component. Sulfur deficiency limits the utilization of nitrogen by microorganisms. The introduction of sulfur compounds into the environment stimulates microbial synthesis in general, including sulfur-containing amino acids. As a result of incubation of the biosubstrate for 3 days at a temperature of 18 °C, the synthesis of most amino acids by microbial association was activated. Incubation with sodium sulfate (at a dose of 0.3% by weight of biosubstrate) for 3 days at 25 °C had the best stimulating effect on amino acid biosynthesis. Such a biosubstrate can be used as a source of amino acids in the form of a fertilizer or feed additive. In the XXI century anthropogenic impact causes an imbalance of the ecological situation, one of the manifestations of which is the reduction of soil fertility, which can be restored by the introduction of mineral and organic fertilizers, as well as preparations containing amino acids and stimulate seed germination, increase the yield, fertility, and fertility. Amino acids are used as additives in food production, in the treatment of diseases of different etiologies and for other purposes.

2006 ◽  
Vol 400 (1) ◽  
Author(s):  
Susan M. Hutson

BCAAs (branched-chain amino acids) are indispensable (essential) amino acids that are required for body protein synthesis. Indispensable amino acids cannot be synthesized by the body and must be acquired from the diet. The BCAA leucine provides hormone-like signals to tissues such as skeletal muscle, indicating overall nutrient sufficiency. BCAA metabolism provides an important transport system to move nitrogen throughout the body for the synthesis of dispensable (non-essential) amino acids, including the neurotransmitter glutamate in the central nervous system. BCAA metabolism is tightly regulated to maintain levels high enough to support these important functions, but at the same time excesses are prevented via stimulation of irreversible disposal pathways. It is well known from inborn errors of BCAA metabolism that dysregulation of the BCAA catabolic pathways that leads to excess BCAAs and their α-keto acid metabolites results in neural dysfunction. In this issue of Biochemical Journal, Joshi and colleagues have disrupted the murine BDK (branched-chain α-keto acid dehydrogenase kinase) gene. This enzyme serves as the brake on BCAA catabolism. The impaired growth and neurological abnormalities observed in this animal show conclusively the importance of tight regulation of indispensable amino acid metabolism.


1974 ◽  
Vol 31 (1) ◽  
pp. 47-57 ◽  
Author(s):  
A. K. Said ◽  
D. M. Hegsted ◽  
K. C. Hayes

1. Adult rats were fed on diets free of either lysine, methionine, threonine or protein. The threonine- and protein-deficient animals lost weight at approximately the same rate, about 100 g in 14 weeks, at which time several were moribund. In contrast, lysine-deficient animals lost only about 30 g in 14 weeks and had lost only 46 g after 22 weeks, when they were killed. Methionine-deficient animals showed an intermediate response. Losses in weight of several tissues – kidney, heart and two muscles – were related to, but not necessarily proportional to, the loss of body-weight. Liver weights relative to body-weights were large in lysine- and threonine-deficient animals and smallest in methionine-deficient animals.2. Adult rats were fed on diets containing zero, a moderate amount (about twice the estimated minimal requirement) or an excess (about four times the estimated requirement) of lysine or threonine in all combinations (3 × 3 design). Analysis of variance of the body-weights, tissue weights and tissue nitrogen contents indicated, in general, a significant effect of each amino acid, as expected, but also, in most instances, a significant interaction. Plasma concentrations of lysine and threonine were affected by the intakes of the respective amino acids, but plasma lysine concentrations were also affected by the threonine intake.3. Liver histology also suggested significant interactions between the two amino acids. Animals given no lysine but moderate amounts of threonine developed severely fatty livers; next most severely affected were animals receiving excess of both amino acids. Threonine deficiency, in the presence or absence of lysine, produced moderately fatty livers similar to those seen in protein-deficient animals.4. Since animals have varying ability to conserve body nitrogen when they are fed on diets limiting in different essential amino acids, measurements of biological value (BV) and net protein utilization by conventional methods, over a short period of time, over-estimate nutritive value relative to amino acid score and probably over-estimate the true nutritive value of poor-quality proteins, particularly those limiting in lysine. If so, this is a serious error, since it leads to underestimates of the protein requirements if BV is used. The fact that certain tissues, particularly the liver, do not necessarily lose nitrogen in proportion to total body nitrogen and may show specific pathological effects depending on the limiting amino acid or the proportions of amino acids in the diet also indicates that general measures of nitrogen economy may not be sufficiently discriminating tests of the nutritive value of proteins.


2018 ◽  
Vol 12 (2) ◽  
Author(s):  
O. Fursik ◽  
I. Strashynskiy ◽  
V. Pasichny ◽  
О. Kochubei-Lytvynenko

. In the article, the data are given of research carried out in vitro to determine the amino acid composition and the degree of digestibility of the reference and experimental samples of cooked sausage, with the use of the protein-containing composition developed. The protein digestibility-corrected amino acid score (PDCAAS) has been calculated to clarify the assimilation of amino acids that enter the body as part of proteins in experimental cooked sausage samples.It has been established that replacing a part of the meat raw material with the protein-containing composition in the formula of cooked sausages does not affect significantly the amino acid composition of the finished product. The addition of mechanically deboned poultry meat reduces the amount of such essential amino acids as isoleucine by 68 %, compared with the control formula, leucine by 38 %, and valine by 48 %. At the same time, the content of lysine significantly increases by 1.5 times.  The in vitro index of digestibility for an experimental sample of cooked sausages with protein-containing composition at the pepsinolysis stage is slightly reduced compared with the reference sample (by an average of 7 %). At the second stage of hydrolysis (trypsin enzyme), this parameter does not differ from the reference one. During the two stages of hydrolysis, this parameter, with mechanically deboned poultry meat introduced, decreased by an average of 20 %, compared with the reference sample.Calculated PDCAAS has allowed establishing that the true efficiency of proteins in cooked sausages is different from the in vitro index of digestibility, which is due to the presence of limiting values of the essential amino acids content in the product.


Author(s):  
U. Ch. Сhomanov ◽  
G. E. Zhumalieva ◽  
M. Ch Tultabayev ◽  
G. S. Aktokalova ◽  
R. K. Kassimbek ◽  
...  

In this paper, the amino acid composition of protein filling for extruded grain products is studied. According to research, essential amino acids make up more than 1/3 of all amino acids, which means that the protein filling is of high quality. The article considers covering the daily requirement of amino acids of the body with protein filling. It was found that the protein filling contains a rich amino acid composition, and allows you to get extruded grain products with a long shelf life.


Author(s):  
Y. Poberezhets

The research has proved that the usage of probiotyk in feeding of broiler chicken facilitate increasing of the digestibility of amino acids including irreplaceable. Revealed that the digestibility of amino acids was highest in broilers consumption average dose supplements investigated. It should be noted that the increased digestibility of the essential probiotic amino acids, such as: lysine, histidine, arginine, valine, methionine, isoleucine, leucine. Thus, the use of probiotic supplements improve the usefulness of protein supply. Such positive changes result in the increasing of broiler efficiency. The research has proved that the usage of different doses of probiotic supplements «Entero-active» has positively effects on amino acid content in meat of broiler chickens. Specifically, the thoracic and femoral muscles of poultry raised the level of most essential amino acids. Thus, consumption of probiotic feed broilers improves meat quality.


2013 ◽  
Vol 796 ◽  
pp. 21-24
Author(s):  
Yu Qiao Jin ◽  
Bing Li

In order to study eri-silkworm Cordyceps militaris culture condition and the amino acid composition features in different parts of it, this experiment optimized eri-silkworm Cordyceps militaris artificial culture conditions, and determined the amino acid composition in different parts. The results showed that the inoculated eri-silkworm Cordyceps militaris which was kept at low temperature (18 ° C) and seventeen dark days grew well and began to use natural light in the eighteenth day with humidity 85%, temperature 21 ° C, which could induce the germination of the sporophore. The artificial cultivation period was about 40 days. Amino acid test shows that its sporophore and stroma are rich in 17 kinds of amino acids, including 7 kinds of essential amino acids. The higher levels of amino acids in its sporophore are lysine 15.28%, glutamate 15.05% and aspartate 7.55%. The higher levels of the amino acids in the stroma are glycine 16.11%, lysine 9.16% and glutamate 8.30%. The results indicate that eri-silkworm Cordyceps militaris can be artificially cultured. As there are differences of amino acid composition in different parts of eri-silkworm Cordyceps militaris, this study lays the foundation for its large-scale cultivation and the exploitation of its amino acid nutrition.


2021 ◽  
Author(s):  
Sarah L Gautrey ◽  
Mirre J P Simons

Dietary restriction (DR) is one of the most potent ways to extend health- and lifespan. Key progress in understanding the mechanisms of DR, and ageing more generally, was made when dietary protein, and more specifically essential amino acids (EAA), were identified as the key dietary component to restrict to obtain DRs health and lifespan benefits. This role of dietary amino acids has strongly influenced work on ageing mechanisms, especially in nutrient sensing, e.g. Tor and insulin(-like) signalling networks. Experimental biology in Drosophila melanogaster has been instrumental in generating and confirming the now dominant hypothesis that EAA availability is central to ageing. Here, we expand on previous work testing the involvement of EAA in DR through large scale (N=6,238) supplementation experiments across four diets and two genotypes in female flies. Surprisingly, we find that EAA are not essential to DRs lifespan benefits. Importantly, we do identify the fecundity benefits of EAA supplementation suggesting the supplemented EAA were bioavailable. Furthermore, we find that the effects of amino acids on lifespan vary by diet and genetic line studied and that at our most restricted diet fecundity is constrained by other nutrients than EAA. We suggest that DR for optimal health is a concert of nutritional effects, orchestrated by genetic, diet and environmental interactions. Our results question the universal importance of amino acid availability in the biology of ageing and DR.


2018 ◽  
Vol 20 (88) ◽  
pp. 69-71
Author(s):  
I. S. Danilova

Amino acids are organic compounds that are the structural component of the protein. That is, protein is the main building material of the tissues of the body. It is needed for muscle mass growth and is indispensable in fat burning – all about amino acids, of which protein is formed. The main source of amino acids are food that is rich in protein. However, based on the content of one or another amino acid proteins contained in food, can be divided into complete and inferior. Complete proteins contain all the essential amino acids. These products include, primarily, products of animal origin: meat, poultry, fish, eggs, dairy products. In order to plant sources of full protein is soybean. Inferior proteins – in their composition there is at least one irreplaceable acid. Accordingly, in their “quality” defective proteins can be very different. The source of defective proteins is mainly products of planting: legumes, cereals, nuts and seeds. The purpose of our work was to determine the content of amino acids in raw and cooked meat of the food species Helix pomatia, Helix aspersa maxima and Helix aspersa muller and to make an analysis on the effect of heat treatment on the amino acid composition of snail meat. This article presents the results of the content of amino acids in meat of food snails using electrophoresis “Capel-105/105M”. Three types of snails have identified the presence and amount of amino acids. In general, the meat of snails contains amino acids – arginine, histidine, serine, alanine, glycine, tyrosine and proline, and irreplaceable – lysine, phenylalanine, leucine + isoleucine, methionine, valine, threonine.


2020 ◽  
pp. 62-66
Author(s):  
Ilya Anatolyevich Kibkalo Ilya ◽  
Olga Borisovna Kameneva ◽  
Ekaterina Aleksandrovna Zhuk ◽  
Vera Valeryevna Bychkova

The amino acid composition of protein in mixed feed samples of experimental formulations for young cattle is Analyzed. The variation of the content of individual amino acids depending on the component composition of the feed was revealed. The possibilities of combining the high content of individual essential amino acids in one version of feed are considered.


Author(s):  
N. Vovkotrub ◽  
A. Melnyk ◽  
L. Bogatko ◽  
O. Piddubnyak ◽  
S. Sokolenko

It has been established that the level of blood parameters in the body of sheep is not always stable and often depends on the breed, sex, their physiological state, the level of productivity, feeding and housing conditions. Among the factors that determine the optimal sheep nutritional level, not the last place is occupied by their provision with a sufficient amount of essential amino acids, especially lysine, methionine, cystine, and vitamins, given their physiological importance for the animal body. The overwhelming amount of them is able to be synthesized in the sheep rumen with the help of microorganisms of the gastrointestinal tract, however, some of them are irreplaceable, that is, those whose balance must be replenished through feed rations. The article describes the use of the vitamin-amino acid complex "Аlphabet for Animals" in sheep, containing a complex of fat-and water-soluble vitamins, as well as amino acids, which contribute to the normalization of metabolism in the body of small ruminants, increase its resistance, have a positive effect on productivity, safety and reproductive animals functions. During the research, the effect of this feed additive on hemocytopoiesis and the exchange of essential micronutrients in ewes were studied. After a double use of the additive, an increase in the processes of erythrocytopoiesis was established, as evidenced by an increase in the number of blood erythrocytes by 21.2% and the hemoglobin content by 11.2%, stabilization of the size and degree of saturation of erythrocytes with hemoglobin, as evidenced by a decrease in MCH and MCV indices. There were no significant changes in the content of essential micronutrients in the blood serum of sheep under the influence of the "Alphabet for Animals", and a slight increase of zinc content in the blood was noted in sheep after the second injection of the drug. At the same time, in the blood of experimental animals group a significant increase the iron content on average up to 129.5±7.33 μg/100 ml (p<0.05) was established, and the number of animals with a low amount of blood iron was only 12.5 %. The number of leukocytes in the sheep blood of both groups was within the normal range and during the second and third blood tests did not change significantly. Key words: sheep, feeding, feed additives, amino acids, vitamins, hemocytopoiesis, essential micronutrients.


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