scholarly journals Investigation of the parameters of acute and acute toxicity of the “VetMicoDerm” liniment under the skin application

2018 ◽  
Vol 20 (92) ◽  
pp. 59-63
Author(s):  
V. Martynyshyn
Keyword(s):  
Body Weight ◽  
White Blood Cells ◽  
Skin Toxicity ◽  
Functional Condition ◽  
The Body ◽  
Skin Area ◽  
Experimental Animals ◽  
Laboratory Rats ◽  
Grade 5 ◽  
External Stimuli

The toxicity parameters of “VetMikoDerm” for one-time and long-term skin treatment were investigated. The studied liniment as an off-white substance has a thio derivative of 1,2,4-triasole.  Spotted thistle oil is used as a solvent. The research of the determination of acute and low acute skin toxicity has been performed on young, healthy, healthy skin of the laboratory rats. The drug was applied in different doses to the stripped areas of the skin (area is not less than 10% of the total area of the skin). It was established that after a single application of the drug (24 hours) in a dose of less than 2000 mg/kg of body weight, the death of experimental animals or any signs of general intoxication of the body is not observed.  Local reaction (redness, itching, allergy, combing) is absent. According to GHS under the assessment in this dose studying the “VetMicoDerm” drug belongs to the 5th safety category (Grade 5, not classified). During a long-lasting application of the drug (28 days), the death of the rats was not adversed, the animals had a preserved appetite, responded adequately to external stimuli, reflex excitability was completely preserved.  Under hematological and biochemical studies of blood of the experimental animals, it was found that against the backdrop of insignificant increase in the number of white blood cells in their blood, the number of neutrophils increases and the percentage of lymphocytes decreases significantly. However, under the usage of the drug in the therapeutic dose (50 mg/kg body weight) the liver function has a tendency to increase. Increasing in dose (500 mg/kg) of the VetMikoDerm a 10-fold continued its application which  led to an increase in serum concentrations of urea and creatinine,increasing  the mass index can serve as a marker of renal decline functional condition of the body.

Toxicological characteristics of a medicinal product for veterinary «Ketoprofen 10%» in laboratory rats (subchronic toxicity)

2021 ◽  
Vol 1 (6) ◽  
pp. 34-41
Author(s):  
A. N. Shkatova ◽  
◽  
D. A. Devrishov ◽  
O. B. Litvinov ◽  
V. E. Brylina ◽  
...  
Keyword(s):  
Body Weight ◽  
Medicinal Product ◽  
Intramuscular Injection ◽  
Study Drug ◽  
The Body ◽  
Subchronic Toxicity ◽  
Internal Organs ◽  
Experimental Animals ◽  
Laboratory Rats

In a subchronic experiment with intramuscular injection in rats, it was found that a dose of 70,7 mg/kg is toxic, and doses of 35,4 and 17,2 mg/kg are inactive (safe). It was noted that the 14 day administration of the drug «Ketoprofen 10%» in a dose 70,7 mg/kg caused a decrease in the body weight of experimental animals, contributed to the development of hepatotoxic action, a change in the mass coefficients of internal organs. The above effects were reversible 10 days after discontinuation of the study drug.

scholarly journals Toxicity assessment of a technical product of the triazole class

2020 ◽  
Vol 99 (11) ◽  
pp. 1276-1279
Author(s):  
Valery N. Rakitskii ◽  
Tatiana M. Epishina ◽  
Elena G. Chkhvirkiya
Keyword(s):  
Body Weight ◽  
The Body ◽  
Male Rats ◽  
Acute Oral Toxicity ◽  
Oral Toxicity ◽  
High Biological Activity ◽  
Experimental Animals ◽  
Technical Product ◽  
White Rats ◽  
Toxicological Studies

Introduction. Historically, pesticides are evaluated more strictly from a medical point of view than other chemicals. Since their features, such as deliberate introduction into the environment, the possibility of contact with them by large masses of the population, and the high biological activity determine their potential danger to humans. Purpose of research - study of the biological effect of a technical product derived from triazoles when it is repeatedly ingested orally in mammals (rats), establishment of inactive and active doses, justification of the permissible daily dose (DSD) for humans. Material and methods. In acute experiments, white rats were used, including 6 animals in the group. Tested dose: 500-4000 mg/kg of body weight. A chronic (12 months) experiment was performed on 80 male rats with a bodyweight of 180-190 g at the beginning of the study. Tested doses: 5.0; 16.0 and 55.0 mg/kg of body weight (1 control and 3 experimental animals, 20 individuals each). In the dynamics of the experiment, we observed the condition and behavior of animals, water, and food consumption, recorded the timing of death, changes in body weight, physiological, biochemical, and hematological indices. Results. Indices of the acute oral toxicity on the studied product LD50 male rats were 2250 ± 483 mg/kg body weight. The dose of 5.0 mg / kg of body weight was not found to cause significant changes in all studied indices. The doses of 16.0 and 55.0 mg/kg of body weight had a polytropic effect on the body in experimental animals. Discussion. The studied product for the acute oral toxicity refers to low-hazard compounds, the doses of 16.0 and 55.0 mg/kg of body weight has a polytropic effect on the mammalian body, causing changes in carbohydrate, lipid, and lipoprotein metabolism in the body of rats - was accepted as acting. The dose of 5.0 mg / kg of body weight, when administered in rats, there are no changes in all the studied parameters throughout the experiment, is accepted as invalid. Based on the inactive dose-5.0 mg/kg of body weight and taking into account the reserve factor of 100, we have scientifically justified DSD for a person at the level of 0.05 mg/kg. Summary. The conducted sanitary and Toxicological studies indicate the need to assess the toxicity of new technical products to the mammalian body, to increase the reliability of the developed hygiene standards in environmental objects and food products.

Effect of Bitters on the Body Weight, Lipid Profile, Catalase and Lipid Peroxidation in Experimental Animals

2012 ◽  
Vol 13 (1) ◽  
pp. 62-66 ◽  
Author(s):  
M.A. Alabi ◽  
R.M. Sunday ◽  
T. Olowokere ◽  
F.A. Kareem ◽  
F. Osanaiye
Keyword(s):  
Lipid Peroxidation ◽  
Body Weight ◽  
Lipid Profile ◽  
The Body ◽  
Experimental Animals

SH2B1 CpG-SNP Is Associated with Body Weight Reduction in Obese Subjects Following a Dietary Restriction Program

2014 ◽  
Vol 66 (1) ◽  
pp. 1-9 ◽  
Author(s):  
Maria Luisa Mansego ◽  
Fermin Ignacio Milagro ◽  
Maria Angeles Zulet ◽  
José Alfredo Martinez
Keyword(s):  
Dna Methylation ◽  
Body Weight ◽  
Weight Reduction ◽  
Molecular Mechanisms ◽  
White Blood Cells ◽  
The Body ◽  
Mrna Levels ◽  
Bdnf Mrna ◽  
Body Weight Reduction ◽  
Obese Subjects

The objective of this study was to examine whether 7 SNPs previously associated with obesity-related traits that add or remove potential sites of DNA methylation are accompanied by differential DNA methylation and subsequently affect adiposity variables or body weight reduction in WBC from obese subjects under an energy-restricted program. Material and Methods: Anthropometric measurements were assessed in 47 volunteers recruited within the RESMENA study (Spain). At baseline, DNA from white blood cells was isolated and 7 obesity-related trait CpG-SNPs were genotyped by TaqMan-PCR. Then, methylation levels of CpG-SNP sites were quantified by MassArray® EpiTyper™ or MS-HRM approaches. Results: Differential DNA methylation levels were observed by genotypes in all of the CpG-SNPs analyzed. The FTO and BDNF methylation levels were further correlated with baseline body weight and, BDNF mRNA levels and body weight change, respectively. Moreover, the rs7359397 (SH2B1) was associated with the body weight, body mass index, and truncal fat mass reduction. Conclusions: Our results reveal the interaction of epigenetic and genetic variations in CpG-SNPs, especially in BDNF and SH2B1 genes, and how allele-specific methylation may contribute to elucidate the possible molecular mechanisms as these SNPs are affecting the decrease of mRNA levels and contributing to a lower body weight reduction. © 2014 S. Karger AG, Basel

scholarly journals FURTHER STUDIES ON EFFECTS OF TISSUE EXTRACTS ON STAPHYLOCOCCUS AUREUS

1946 ◽  
Vol 84 (3) ◽  
pp. 247-261 ◽  
Author(s):  
Leo G. Nutini ◽  
Sister Eva Maria Lynch
Keyword(s):  
Staphylococcus Aureus ◽  
Body Weight ◽  
The Body ◽  
Brain Extract ◽  
Toxicity Tests ◽  
Control Series ◽  
Experimental Animals ◽  
Tissue Extracts

1. The ability of alcoholic-precipitated extracts of beef tissue—brain, spleen, heart, and kidney—to stimulate the growth of Staphylococcus aureus, in vitro, and to convert the yellow S form to a white R variant with altered biochemical characteristics conforming to those of an avirulent organism, has been confirmed. 2. The avirulence of the white R variant has been established by tests in vivo on mice. 3. Staphylococcus aureus infections induced subcutaneously, intraperitoneally, and intravenously in mice responded favorably to brain extract following subcutaneous or oral administration. The mortality was 2 per cent in 444 experimental animals and 81 per cent in 448 control animals. 4. The extracts appeared equally efficient when used therapeutically (mortality 2 per cent of 162 experimental animals and 90 per cent in the control series) or prophylactically (mortality 2 per cent of 282 experimental animals and 76 per cent in 286 control mice). Extracts of brain and spleen were more effective than those of either heart or kidney. 5. Studies concerning the mechanism of action of the tissue extracts indicate that they prevented the formation of toxin by Staphylococcus aureus, and had but little effect on toxin actions. 6. Toxicity tests revealed that the brain and spleen extracts were relatively non-toxic, dosages equivalent to 2 per cent of the body weight being well tolerated. Kidney and heart extracts were much more toxic, producing mortality in dosages as low as 0.3 per cent of the body weight.

Impact of Increased Body Weight on Acute Toxicity and Outcome of Autologous Stem Cell Transplantation for Non-Hodgkin’s Lymphoma: A Southwest Oncology Group Analysis.

Blood ◽  
2004 ◽  
Vol 104 (11) ◽  
pp. 352-352 ◽  
Author(s):  
Patrick Stiff ◽  
Joseph Unger ◽  
Stephen Forman ◽  
Michael LeBlanc ◽  
Thomas Miller ◽  
...  
Keyword(s):  
Body Weight ◽  
Stem Cell ◽  
Actual Body ◽  
Death Rate ◽  
Skin Toxicity ◽  
High Dose ◽  
Cell Transplant ◽  
Actual Body Weight ◽  
Grade 5 ◽  
Grade 3

Abstract Little is known about the optimal dosing of chemotherapy agents in patients significantly above their ideal body weight (IBW) who undergo high dose therapy with an autologous or allogeneic hematopoietic stem cell transplant. While dose attenuation is often advocated for overweight patients and appears to reduce acute toxicities, its effect on progression-free and overall survival (PFS/OS) is unknown. Due to a high relapse rate after autografts for relapsed/refractory NHL, SWOG has long investigated the use of augmented preparative regimens that utilize high-dose etoposide based on actual body weight (ABW) of 60 mg/kg, along with 12 Gy of TBI and cyclophosphamide (100 mg/kg) which is dosed on unadjusted IBW. We determined acute toxicities and PFS/OS using this approach in a recently completed study (S9438) of 358 patients undergoing autografts for relapsed/refractory NHL, comparing all grade 5 toxicities, grade 3–4 skin toxicities (known to be associated with high dose etoposide) and all other grade 4 toxicities in patients in this study based on % above IBW. The Devine formula for IBW with an adjustment for patients <5 feet was used. Patients at or below their IBW were dosed using their actual body weight. All patients received the preparative regimen as stated followed by autologous peripheral blood stem cells. Overall there were 31 patients with grade 5 toxicities. This group was a mean 42% above their IBW vs 24% for those surviving transplant (p=.001). Increasing % above IBW predicted grade 5 toxicity (p=.002). Even those at or 10% above their IBW had a higher toxic death rate (10.1 vs 3.6%; p=.04). While increasing weight above IBW did not predict for grade 4 lung, liver, cardiac toxicities or infections (p=.12), it did predict for grade 3/4 skin toxicities (p< .001). Skin toxicity, most commonly hand/foot syndrome was seen at rates up to 27.3% vs 7.3% for those ≥ 50 vs < 50% over IBW (p < .001). However, even those just ≥ 10% over IBW had nearly a 4 fold higher risk of skin toxicity (13.4 vs 3.6%; p=.005). While survival by % over IBW is confounded by comorbidities in overweight patients, we found no evidence of a different 2-year PFS or OS for overweight patients, even those ≥ 50% above IBW (PFS=42 vs 45%, p = .28; OS=52 vs 62%, p = .38). These multicenter trial data do establish a correlation of the ABW dosing of etoposide with significant skin toxicity. Without an apparent later PFS/OS benefit to the use of unadjusted etoposide dosing for overweight patients but higher acute skin toxicity and a higher early death rate, etoposide will be dosed using adjusted IBW [ IBW + .4 (ABW − IBW)] in subsequent studies of this regimen.

Acute and sub-chronic oral toxicities of DA.AMLODEPON HVD hard capsule in experimental animals

2021 ◽  
Vol 148 (12) ◽  
pp. 58-67
Author(s):  
Pham Thi Van Anh ◽  
Nguyen Van Dam ◽  
Nguyen Van Dat ◽  
Pham Thanh Ky ◽  
Nguyen Trong Thong ◽  
...  
Keyword(s):  
Body Weight ◽  
Acute Toxicity ◽  
Chronic Toxicity ◽  
The Body ◽  
Toxicity Study ◽  
World Health ◽  
Histopathological Analysis ◽  
Experimental Animals ◽  
Health Organization ◽  
General Conditions

Assessment of toxicities of DA.AMLODEPON HVD hard capsule on experimental animals. The acute toxicity of DA.AMLODEPON HVD was assessed on Swiss mice according to World Health Organization Guidance, and LD50 determination according to the method of Litchfield – Wilcoxon. The sub-chronic toxicity study of DA.AMLODEPON HVD at two doses (0.42 g/kg/day and 1.26g/kg/day) was conducted in rats for four consecutive weeks. After administration, general conditions and the body weight of rats were evaluated. Blood samples were collected for analyzing serum parameters before treatment (T0), second week (T1), and fourth week (T2). Histopathological analysis of livers and kidneys was observed at the end of the experiment. The results revealed that mice were taken up to a maximum dose of 39.15 g/kg with no symptoms of acute toxicity, LD50 of DA.AMLODEPON HVD has not been determined. The sub-chronic toxicity study at two doses did not change the body weight of rats, general conditions. The parameters for structures and functions of livers and kidneys and microscopic of the livers and kidneys are in a normal range during the study period.

Effect of dietary supplementation of vitamin E, zinc, and chromium supplementation on growth performance and hematological characteristics of broiler chickens

2017 ◽  
Author(s):  
Nazim Ali ◽  
Mohammad Akram ◽  
Ahmad Fahim ◽  
Balvir Singh ◽  
Mohammad Imran
Keyword(s):  
Body Weight ◽  
Vitamin E ◽  
Growth Performance ◽  
Broiler Chickens ◽  
Body Weight Gain ◽  
White Blood Cells ◽  
The Body ◽  
Broiler Chicks ◽  
Feed Conversion ◽  
Wood Shavings

The study investigated the effect of supplementation of Vitamin E, Zn and Cr on growth performance and hematological characteristics of broiler chickens. The 240 day old Cobb 400 chicks were randomly distributed in eight groups consisting of 30 chicks in each. These chicks were kept on deep litter houses comprising of wood shavings. The supplementation of Vitamin E, zinc and chromium either individually or in combination improved the feed conversion ratio and body weight gain in broiler chicks. The body weight of broiler chickens at different age from day old to the 6th week were significantly different among treatments. The highest body weight was recorded in T8 groups. Heamoglobin, red blood corpuscles and white blood cells levels increased whereas heterophil: lymphocyte ratio statistically decreased with vitamin E, zinc or chromium supplemented groups either individually or in combinations.

DYNAMICS OF PROTEIN METABOLISM AND ACTIVITY OF AMINOTRANSFERASES OF DOGS BY ADDING DIHYDROQUERCETIN

2016 ◽  
Vol 1 (2) ◽  
pp. 60-63
Author(s):  
Полищук ◽  
Sergey Polishchuk ◽  
Молянова ◽  
Galina Molyanova
Keyword(s):  
Body Weight ◽  
Blood Cells ◽  
Hematological Parameters ◽  
White Blood Cells ◽  
The Body ◽  
German Shepherd ◽  
Physiological Indicators ◽  
Formalin Edema ◽  
And Function ◽  
Experimental Group

The purpose of research is to increase protective and adaptive reactions, the service and the working potential of an organism of dogs through the use of DHQ. The article presents the results of an experiment to the effect of DHQ on the dynamics of hematological parameters of dogs blood. Dihydroquercetin – an active antioxidant, a natural scavenger of oxygen free radicals, hepatoprotective, has anti-inflammatory action due to limitations of the formalin edema and histamine, and inhibits the formation of serous fluid, pain killers, immunocorrectional properties. Due to the high complexing properties it displays the body of heavy metals, including radionuclides, it helps to restore the tone of blood vessels, normalization of the lipid spectrum of the blood and slows the development of atherosclerotic plaques. Investigations were carried out in Dogs State Service Ministry of I A of Russia zonal center in Samara Region by clinically healthy of dog breed German Shepherd, age 2-4 years with an average body weight of 30 kg on the background conditions and feeding, adopted by the enterprise. Dogs experimental group received Dihydroquercetin to 0.001 g/kg body weight dose once a day with food. When added to the basic diet Dihydroquercetin dog experimental group show an increase in indicators such as red blood cells – by 18.3% (p&#60;0.01), hemoglobin – by 11.7% (p &#60;0.01) hematocrit – 7.1% (p&#60;0.01), white blood cells – by 9.1% (p&#60;0.05) compared to control data. The research results give reason to believe that the use of dietary supplements in Dihydroquercetin in dogs diet dose 0.001 g/kg allows significantly increase the morphological and physiological indicators of animal organism. In this case a significant increase erythrocytes and hemoglobin and hematocrit indicates increased oxidative metabolism and function of the intensity of the blood in dogs treated with the drug, and increase in the blood indicates an increase in immune status.

The effect of the drug "Hepaton" on the reaction of lipid peroxidation

2020 ◽  
Vol 2 ◽  
pp. 112-115
Author(s):  
V.S. Ponamarev ◽  
◽  
O.S. Popova ◽  
Keyword(s):  
Lipid Peroxidation ◽  
Body Weight ◽  
Toxic Hepatitis ◽  
Antioxidant Properties ◽  
The Body ◽  
Intragastric Administration ◽  
Bile Formation ◽  
Laboratory Rats ◽  
Acute Toxic Hepatitis ◽  
Acute Toxic

The composition of the drug "Hepaton" includes many bioactive compounds that provide the antioxidant properties of the drug, manifested by the neutralization of reactive oxygen species (ROS) with the breakaway chain free radical reactions. The aim of the study was to determine the effect of the Hepaton preparation on lipid peroxidation reactions in laboratory rats. Evaluation of the antioxidant effect of the drug was carried out on twenty laboratory rats of both sexes with a body weight of 180-220 g, divided into 2 groups. Acute toxic hepatitis in rats was induced by a single intragastric administration of 1.0 ml of a dichloroethane solution. At the same time, rats of the experimental group (n = 10) 1 hour before the introduction of di-chloroethane were injected with a solution of the drug “Hepaton” in the amount of 10 ml / kg body weight and then 1 time per day for 21 days after the use of toxicants [1]. On the 21st day after the administration of toxicants, a blood was drawn for a biochemi-cal study, which took into account the pa-rameters of the antioxidant system (diene conjugates (DC), ketodienes (CD) and malondialdehyde (MDA), as well as the level of endogenous intoxication (according to the content of MSM). Based on the results obtained, it can be con-cluded that the use of “Hepaton” in modeling acute toxic hepatitis made it possible to re-store the disturbed homeostasis of the labora-tory animal organism, the structure and in-tegrity of the hepatocyte membranes, inhibit lipid peroxidation as one of the links in the pathogenesis of hepatitis, stimulate antioxi-dant defense and power the endogenous anti-oxidant system of the body, bile formation and biliary excretion, as well as activate the reparative processes of the liver tissue at the cellular and intracellular levels.

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