scholarly journals Fusobacterium Nucleatum : Bakteri Anaerob pada Lingkungan Kaya Oksigen (Dihubungkan dengan Staterin Saliva)

2018 ◽  
Vol 1 (1) ◽  
pp. 181-188
Author(s):  
Yumi Lindawati ◽  
Ameta Primasari ◽  
Dwi Suryanto
Keyword(s):  
Bacterial Colonization ◽  
Anaerobic Bacteria ◽  
Fusobacterium Nucleatum ◽  
Cross Sectional ◽  
Obligate Anaerobic ◽  
Supragingival Plaque ◽  
Whole Saliva ◽  
Dental Plaques ◽  
Cross Sectional Design ◽  
Unstimulated Whole Saliva

Fusobacterium nucleatum pada plak gigi berperan penting sebagai jembatan peralihan antara kolonisasi bakteri awal plak gigi dengan kolonisasi bakteri berikutnya, khususnya bakteri anaerob obligat. Fusobacterium nucleatum merupakan bakteri anaerob obligat, namun membran terluar F. nucleatum secara spesifik mengikat asam amino ke-21 dan 26 dari molekul staterin yang berasal dari saliva sehingga memungkinkan bakteri ini dapat tumbuh pada saliva dan plak supragingiva. Penelitian ini bertujuan untuk mengetahui keberadaan dan jumlah koloni F. nucleatum, konsentrasi staterin, korelasi keduanya pada saliva dan plak supragingiva. Penelitian observasional ini menggunakan desain cross- sectional pada 28 sampel Unstimulated whole saliva menggunakan metode spitting dan plak supragingiva matang dilekatkan pada paper point nomor 60 dari pasien di Instalasi Periodonsia RSGMP FKG USU. Konsentrasi staterin dihitung menggunakan ELISA, jumlah koloni F. nucleatum dengan mengkultur sampel pada media Crystal Violet Erythromicyn, dilakukan perhitungan manual pada piring petri. Penelitian ini menemukan pertumbuhan koloni F. nucleatum dari sampel saliva dan plak supragingiva dalam jumlah sedikit. Median konsentrasi staterin diperoleh pada saliva 1,5 µg/ml (x=2,35±2,71 µg/ml) pada plak supragingiva 223 µg/ml (x=332±242 µg/ml). Secara teoritis bakteri ini berhubungan dengan staterin, akan tetapi pada penelitian ini secara statistik tidak dapat dikorelasikan pertumbuhannya dengan staterin. Simpulan penelitian ini membuktikan bahwa F. nucleatum dapat diperoleh dari lingkungan yang mengandung oksigen seperti pada saliva dan plak supragingiva, akan tetapi keterkaitannya dengan staterin saliva pada lingkungan tersebut memerlukan penjelasan lebih lanjut. Fusobacteriumnucleatum on dental plaque plays an important role as a transitional bridge between early bacterial colonization of dental plaques and subsequent bacterial colonization, especially obligate anaerobes. Fusobacteriumnucleatum is an obligate anaerobic bacteria, but the outer nucleatum membrane specifically binds to the 21st and 26th amino acids from the statherin molecule which comes from saliva, allowing this bacteria to grow in saliva and supragingival plaque. The objective of this study was to determine the presence and the number of fusobacteriumnucleatum colonies, statherine concentrations, their correlation to saliva and supragingival plaque. This observational study used a cross-sectional design in 28 unstimulated whole saliva samples using spitting and mature supragingival plaque methods attached to number 60 paper points from patients at Installation Periodonsia RGGMP FKG USU. The concentration of statherin was calculated using ELISA, the number of F. nucleatum colonies by culturing samples on the Violet Erythromicyn Crystal media. Manual calculations were made on petri dishes. This study found small amounts of F. nucleatum colonies from saliva and supragingival plaque samples. The median statherine concentration was found at saliva 1.5 µg / ml (x = 2.35 ± 2.71 µg / ml) and at supragingival plaques 223 µg / ml (x = 332 ± 242 µg / ml). Theoretically, this bacteria is related to statherine, but its growth can not be statistically correlated with statherine in this study. The conclusion of this study proved that F. nucleatumcould be obtained from oxygen rich environment such as saliva and supragingival plaque, but its association with saliva in the temperature required further explanation.

scholarly journals Detection of Salivary miRNAs Reflecting Chronic Periodontitis: A Pilot Study

Molecules ◽  
2019 ◽  
Vol 24 (6) ◽  
pp. 1034 ◽  
Author(s):  
Kohei Fujimori ◽  
Toshiki Yoneda ◽  
Takaaki Tomofuji ◽  
Daisuke Ekuni ◽  
Tetsuji Azuma ◽  
...  
Keyword(s):  
Pilot Study ◽  
Chronic Periodontitis ◽  
Pocket Depth ◽  
Cross Sectional ◽  
Relative Expression ◽  
Reverse Transcription Pcr ◽  
Probing Pocket Depth ◽  
Severe Periodontitis ◽  
Whole Saliva ◽  
Unstimulated Whole Saliva

The purpose of this cross-sectional pilot study was to find salivary microRNAs (miRNAs) reflecting periodontal condition in chronic periodontitis. One hundred and twenty chronic periodontitis patients (mean age, 68.4 years) participated in the study, from whom unstimulated whole saliva was collected. A multiphase study was conducted to explore salivary miRNAs as biomarkers of periodontitis. At first, a polymerase chain reaction (PCR) array was performed to compare salivary miRNAs profiles in no and mild (no/mild) and severe periodontitis patients. Next, the relative expression of salivary miRNAs on individual samples was assessed by real-time reverse transcription-PCR. The numbers (%) of patients were 26 (21.6%, no/mild), 58 (48.3%, moderate) and 36 (30.0%, severe), respectively. Among 84 miRNAs, only the relative expression of hsa-miR-381-3p in the severe periodontitis group was significantly higher than that of the no/mild periodontitis group (p < 0.05). Among the 120 patients, there was also a significant correlation between the relative expression of hsa-miR-381-3p and the mean probing pocket depth (PPD) (r = 0.181, p < 0.05). Salivary hsa-miR-381-3p was correlated with periodontitis condition in chronic periodontitis patients.

scholarly journals Investigation of Salivary RANKL and OPG Levels in Periodontitis Patients at Hospital Universiti Sains Malaysia

2021 ◽  
Author(s):  
Saba Asif ◽  
Basaruddin Ahmad ◽  
Syed Ameer Hamza ◽  
Haslina Taib ◽  
Nur Karyatee Kassim ◽  
...  
Keyword(s):  
Cross Sectional Study ◽  
Periodontal Pocket ◽  
Pocket Depth ◽  
Cross Sectional ◽  
Plaque Score ◽  
Mean Values ◽  
Severe Periodontitis ◽  
Whole Saliva ◽  
Elisa Technique ◽  
Unstimulated Whole Saliva

Abstract Background This study was aimed to determine the levels of salivary receptor activator of NF-κB ligand (RANKL) and osteoprotegerin (OPG) and its association with periodontal status among periodontitis patients. Patients and Methods A cross-sectional study was designed and performed at the Dental Clinic, Hospital Universiti Sains Malaysia (HUSM). Random sampling was employed to identify 88 participants into three groups: 30 mild periodontitis, 30 moderate to severe periodontitis, and 28 healthy (nonperiodontitis) patients. Periodontal parameters: periodontal pocket depth (PPD), clinical attachment level (CAL), plaque score (PS), and gingival bleeding index (GBI) were recorded. In total, 4 mL of unstimulated whole saliva was collected to determine the levels of salivary RANKL and OPG proteins by using ELISA technique. Data were analyzed by using SPSS software version 24.0. Results Mean values for PPD (5.3 ± 0.5) and CAL (5.6 ± 0.5) were observed higher for moderate to severe periodontitis as compared with values (4.4 ± 0.2) (4.5 ± 0.2) in mild periodontitis patients. The mean salivary RANKL and OPG was 0.23 ± 0.07 ng/mL and 1.78 ± 0.70 ng/mL respectively in moderate to severe periodontitis. Only salivary RANKL levels were significantly and positively correlated with all the clinical periodontal parameters. Conclusion The levels of salivary RANKL were higher as opposed to lower OPG levels in periodontitis patients in contrast to healthy (nonperiodontitis) patients. RANKL levels were significantly associated with the periodontal parameters. Therefore, we can conclude that RANKL can potentially aid as an adjunctive diagnostic protein in evaluating periodontal disease.

Evaluation of the Microbiological Profile of Alveolar Residual Screws and Cleft-Adjacent Teeth in Individuals With Complete Unilateral Fissures

2020 ◽  
Vol 57 (10) ◽  
pp. 1182-1189
Author(s):  
Ronaldo Iurovschi ◽  
Claudia Regina Joaquim ◽  
Marcelo de Faveri ◽  
Tamires Szeremeske de Miranda ◽  
Magda Feres ◽  
...  
Keyword(s):  
Bacterial Species ◽  
Fusobacterium Nucleatum ◽  
Wilcoxon Test ◽  
Hybridization Technique ◽  
Cross Sectional ◽  
Prevotella Nigrescens ◽  
Prevotella Melaninogenica ◽  
Neisseria Mucosa ◽  
Cross Sectional Design ◽  
Prevalent Species

Objective: To evaluate the microbiota profile of residual alveolar slits and teeth adjacent to the cleft in fissured individuals. Designs: This study used a cross-sectional design. Participants: Twenty individuals, aged 14 to 24 years, who had a residual fissure in the maxillary alveolar ridge region were selected. Main outcome measures: Three sites per individual were selected for microbiological collection (the site of the residual cleft and the 2 nearest teeth). The samples were analyzed using the Checkerboard DNA–DNA hybridization technique for 73 species of bacteria. Results: All the species analyzed were found in the 2 niches (slits and teeth). The bacterial species present in the largest number in the residual cracks were Prevotella melaninogenica, Prevotella nigrescens, and Streptococcus mitis. With regard to the bacterial profiles in the mesial and distal faces, the most prevalent species were P nigrescens, Veillonella parvula, and Fusobacterium nucleatum sp vicentii. The analysis of all the collected samples demonstrated very similar profiles for the mesial and distal faces, with these 2 sites even presenting the same species in greater frequencies. Higher counts of 20 bacterial species (Wilcoxon test) were observed in the dental niche, in relation to the fissure, particularly, P nigrescens, V parvula, F nucleatum sp vicentii, and Neisseria mucosa. Conclusion: Some species were significantly more prevalent in the residual alveolar fissures and in adjacent teeth. The comparison between the profiles of the 2 niches demonstrated large differences in the most frequent species in the teeth, and no qualitative differences with regard to specific pathogens.

Fluoride in Dental Biofilm Varies across Intra-Oral Regions

2017 ◽  
Vol 51 (4) ◽  
pp. 402-409 ◽  
Author(s):  
Line Staun Larsen ◽  
Vibeke Baelum ◽  
Livia Maria Andaló Tenuta ◽  
Alan Richards ◽  
Bente Nyvad
Keyword(s):  
Sampling Site ◽  
Fluoride Concentration ◽  
Cross Sectional Study ◽  
Anterior Region ◽  
Cross Sectional ◽  
Dental Biofilm ◽  
Whole Saliva ◽  
Biofilm Fluid ◽  
Fluoride Distribution ◽  
Unstimulated Whole Saliva

Information on differences in biofilm fluoride concentration across intra-oral regions may help explain the distribution of caries within the dentition. The aim of this cross-sectional study was to describe the fluoride concentration in saliva and in biofilm fluid and biofilm solids across 6 intra-oral regions. Unstimulated whole saliva was collected from 42 participants and biofilm harvested from the buccal sites in the 4 molar and 2 anterior regions. Samples were collected at least 1 h after use of fluoride dentifrice. No attempt was made to control the participants' food consumption or use of other topical agents. Centrifuged saliva, biofilm fluid, and biofilm solids were analysed for fluoride using a fluoride ion-selective electrode, adapted for microanalysis. Fluoride in biofilm varied across intra-oral regions. The mean biofilm fluid fluoride concentrations across the oral cavity ranged from 11.6 to 16.8 µM, being statistically significantly higher in the upper anterior region than in any other region. In all regions the fluoride concentration in biofilm fluid was higher than in saliva. For biofilm solids the fluoride concentration was highest in the lower anterior region (2,461 μmol/kg) and lowest in the lower molar regions (388 and 406 μmol/kg, respectively). Within biofilm, the solids contained most of the fluoride (81 to >99%). The biofilm fluid fluoride concentration was significantly positively associated with salivary fluoride and only marginally associated with that of biofilm solids. In conclusion, this study has shown pronounced differences in fluoride distribution across intra-oral regions and compartments. This shows that the sampling site is a crucial factor for studies of biofilm fluoride.

scholarly journals Unstimulated Whole Saliva Cortisol Levels during Ramadan in Iranian Muslims

2014 ◽  
Vol 15 (3) ◽  
pp. 341-344 ◽  
Author(s):  
Iraj Mirzaii-Dizgah ◽  
Behnoosh Vasaghi-Gharamaleki
Keyword(s):  
Repeated Measures ◽  
Cross Sectional Study ◽  
Cortisol Concentration ◽  
Cross Sectional ◽  
Great Opportunity ◽  
Saliva Cortisol ◽  
Whole Saliva ◽  
Unstimulated Saliva ◽  
Cortisol Levels ◽  
Unstimulated Whole Saliva

ABSTRACT Aims Ramadan is a great opportunity for scientific research due to its peculiar nature. This study was designed to investigate whether morning saliva cortisol pick can change during and after Ramadan compared to before it. Materials and methods A total of 30 healthy volunteer men (aged between 30 and 76 years) were asked to participate in a cross-sectional study between August 12 and September 10, 2010. Saliva was collected 2 weeks before the beginning of Ramadan (BR), during the first week (R1), middle (R2), the last week (R3) of Ramadan and 3 weeks after Ramadan (AR). Cortisol concentration was analyzed by ELISA. Statistical analysis of one-way repeated measures analysis of variance (ANOVA) was used. Results The mean unstimulated saliva cortisol concentration and its output were significantly higher in the BR than during and after Ramadan. Conclusion Mean saliva cortisol concentration and its output tended to be lower during and 3 weeks after Ramadan. Clinical significance Cortisol as a stress hormone seems to be low during Ramadan. How to cite this article Vasaghi-Gharamaleki B, Mirzaii-Dizgah I. Unstimulated Whole Saliva Cortisol Levels during Ramadan in Iranian Muslims. J Contemp Dent Pract 2014;15(3):341-344.

scholarly journals ANALYSIS OF CHARACTERISTICS AND AMBIENT AIR SULFIDE HYDROGEN CONCENTRATION RISKQUOTIENTON THE LUNG VITAL CAPACITY OF PEOPLE LIVING AROUND LANDFILL AREA

2018 ◽  
Vol 12 (3) ◽  
Author(s):  
Mohammad Zulkarnain ◽  
Rostika Flora ◽  
Novrikasari Novrikasari ◽  
Toto Harto ◽  
Dwi Apriani ◽  
...  
Keyword(s):  
Air Pollution ◽  
Nutritional Status ◽  
Vital Capacity ◽  
Anaerobic Bacteria ◽  
Ambient Air ◽  
P Value ◽  
Management Service ◽  
Cross Sectional ◽  
Open Dumping ◽  
Cross Sectional Design

AbstractWaste is one of factors causing air pollution in Palembang City. Volume of waste that should be processed increases every day, while condition of waste management service is still 70% of the total volume. The waste processing is managed by using open dumping system, which affects on the increase of air pollution. One of gases that exist as effect of the process of organic compound decomposition of anaerobic bacteria from garbage is sulfide hydrogen (H2S) pollutant which can promote health disorders, especially respiratory system. This study aimed to analyze correlation between characteristics (age, sex, nutritional status, smoking, and living distance) and ambient air H2S concentration risk quotient to lung capacity of people around landfill area. This study used cross-sectional design with the sample of 78 people around landfill area. Data analysis used double logistic regression. Results showed that nutritional status (p value = 0.222, OR = 12.085) and RQ (p value = 0.016; OR = 7.547) significantly related to lung vital capacity of people around landfill area. People around landfill area having worse nutrition and lower RQ than the median were potential to have lung vital capacity disorder. The dominant variable significantly influencing to lung vital capacity of people living around Sukawinatan Landfill is nutritional status.AbstrakSampah merupakan salah satu penyebab terjadinya polusi udara di Kota Palembang. Setiap hari terjadi peningkatan volume sampah yang harus diolah, sedangkan kondisi pelayanan pengelolaan sampah baru mencapai sekitar 70% dari total sampah secara keseluruhan. Pengolahan sampah yang dilakukan menggunakan sistem open dumping berdampak terhadap peningkatan terjadinya polusi udara. Gas hidrogen sulfida (H2S) yang diperoleh dari proses penguraian senyawa organik oleh bakteri anaerob pada tumpukan sampah dapat mengganggu kesehatan, terutama sistem pernapasan. Penelitian ini bertujuan untuk menganalisis hubungan antara karakteristik (usia, jenis kelamin, status gizi, merokok, dan jarak tempat tinggal) dan besaran risiko konsentrasi H2S udara ambien terhadap kapasitas vital paru penduduk di sekitar tempat pembuangan akhir (TPA) sampah. Penelitian ini menggunakan desain potong lintang dengan sampel 78 orang penduduk yang berada di sekitar TPA. Analisis data menggunakan uji regresi logistik ganda. Hasil menunjukkan nilai status gizi (p value = 0,022, OR = 12,085) dan RQ (p value = 0,016; OR = 7,547) berhubungan secara signifikan terhadap kapasitas vital paru penduduk yang berada di sekitar TPA. Penduduk yang berada di sekitar TPA dengan nilai status gizi buruk dan nilai RQ lebih rendah dari median berpotensi mengalami gangguan kapasitas vital paru. Variabel dominan yang secara signifikan memengaruhi kapasitas vital paru penduduk yang berada di sekitar TPA sampah Sukawintan adalah status gizi.

scholarly journals DIFFERENCE OF pH AND CALCIUM LEVEL OF SALIVARYIN CARIES AND CARIES FREE STUDENTS OF FAKULTAS KEDOKTERAN GIGI UNIVERSITAS SUMATERA UTARA

2016 ◽  
Vol 19 (2) ◽  
pp. 128-132
Author(s):  
Lisna Unita R ◽  
Rahayu Nisak
Keyword(s):  
Calcium Level ◽  
Salivary Flow ◽  
Mean Value ◽  
Control Group ◽  
Cross Sectional ◽  
Balanced Diet ◽  
Whole Saliva ◽  
Significant Difference ◽  
Cross Sectional Design ◽  
Tooth Tissue

Dental caries is a disease of hard tooth tissue in enamel, dentin and cementum, caused by bacterial activity that ferments carbohydrates leading to the dissolution of enamel due to acid produced by bacteria. Saliva contains protein, antibacterial, fluoride, calcium and phosphate ions that can protect teeth from caries. Balanced diet and adequate salivary flow will preserve to protect and maintain the health of hard tooth tissue. The caries process begins with Streptococcus mutants fermenting carbohydrates to form acids that produced a low pH of oral cavity. Low concentrated calcium in saliva can increase caries risk. The purpose of this study was to determine the volume, pH and saliva’s calcium level in caries and caries-free (control group) students of Fakultas Kedokteran Gigi Universitas Sumatera Utara. The research was an observational analytic study with cross-sectional design. The saliva studied was stimulated whole saliva taken from 46 subjects, 23 were caries and 23 were caries-free. Subjects were instructed to chew paraffin wax for 5 minutes and spitting the saliva into the pot. The examination to determine salivary volume was conducted in Laboratories of Oral Biology and Fakultas Farmasi Universitas Sumatera Utara. The result of this study showed the mean value of  salivary volume in caries group is  3.55 ± 0.633 ml/5minutes and caries-free is 6.94 ± 1.482 ml/5 minutes, caries saliva pH 5.26 ± 0.219 and caries-free 6.68 ± 0.362, and calcium saliva caries 0.98 ± 0.310 mmol/L and caries-free 1.55 ± 0.312 mmol/L. There was a significant difference (p < 0.05) between the caries group and caries-free in volume, pH and salivary calcium levels. The conclusion of this research is the caries group has lower volume, pH and calcium level of saliva than non caries group.

Relationship of Self-Compassion, Hope, and Emotional Control to Perceived Burdensomeness, Thwarted Belongingness, and Suicidal Ideation

Crisis ◽  
2020 ◽  
pp. 1-7
Author(s):  
Laura R. Umphrey ◽  
John C. Sherblom ◽  
Paulina Swiatkowski
Keyword(s):  
College Students ◽  
Suicidal Ideation ◽  
Emotional Control ◽  
Thwarted Belongingness ◽  
Perceived Burdensomeness ◽  
Cross Sectional ◽  
Self Compassion ◽  
Positive Feelings ◽  
Cross Sectional Design ◽  
Relationship Of

Abstract. Background: Cultivating positive feelings of self in relationships with others can affect perceptions of belongingness and burdensomeness. Aims: The present study examines the relationships of self-compassion, hope, and emotional control to thwarted belongingness, perceived burdensomeness, and suicidal ideation. Method: Participants were 481 college students who completed scales measuring self-compassion, hope, emotional control, thwarted belongingness, perceived burdensomeness, and suicidal ideation. Results: Correlation and parallel mediation analysis results show relationships between self-compassion, hope, and emotional control with perceived burdensomeness, thwarted belongingness, and suicidal ideation. Limitations: The study is limited by its cross-sectional design, sample demographics, and inability to distinguish between individuals with suicidal ideation and those who attempt suicide. Conclusion: The results show that the relationships of self-compassion, hope, and emotional control to perceived burdensomeness, thwarted belongingness, and suicidal ideation are worth further investigation.

Rorschach-CS Scores of Six Groups of Patients

2013 ◽  
Vol 34 (1) ◽  
pp. 24-55 ◽  
Author(s):  
Caspar C. Berghout ◽  
Jolien Zevalkink ◽  
Abraham N. J. Pieters ◽  
Gregory J. Meyer
Keyword(s):  
Mental Health ◽  
Health Care ◽  
Health Care Organizations ◽  
Total Sample ◽  
Psychoanalytic Psychotherapy ◽  
Cross Sectional ◽  
Treatment Groups ◽  
Treatment Type ◽  
Psychotherapy Groups ◽  
Cross Sectional Design

In this study we used a quasiexperimental, cross-sectional design with six cohorts differing in phase of treatment (pretreatment, posttreatment, 2-year posttreatment) and treatment type (psychoanalysis and psychoanalytic psychotherapy) and investigated scores on 39 Rorschach-CS variables. The total sample consisted of 176 participants from four mental health care organizations in The Netherlands. We first examined pretreatment differences between patients entering psychoanalysis and patients entering psychoanalytic psychotherapy. The two treatment groups did not seem to differ substantially before treatment, with the exception of the level of ideational problems. Next, we studied the outcome of psychoanalysis and psychoanalytic psychotherapy by comparing the Rorschach-CS scores of the six groups of patients. In general, we found significant differences between pretreatment and posttreatment on a relatively small number of Rorschach-CS variables. More pre/post differences were found between the psychoanalytic psychotherapy groups than between the psychoanalysis groups. More research is needed to examine whether analyzing clusters of variables might reveal other results.

HUBUNGAN LAMA WAKTU MENDERITA STROKE DENGAN TINGKAT KEMANDIRIAN KLIEN DALAM MELAKUKAN AKTIVITAS PEMENUHAN KEBUTUHAN SEHARI-HARI

2018 ◽  
Vol 1 (2) ◽  
pp. 58
Author(s):  
Setia Budi ◽  
Ria Dila Syahfitri
Keyword(s):  
Data Analysis ◽  
Univariate Analysis ◽  
Sampling Technique ◽  
P Value ◽  
Cross Sectional ◽  
Stroke Incidence ◽  
Bivariate Data ◽  
Cross Sectional Design ◽  
Interviewing Techniques ◽  
Need For Rehabilitation

The rate of stroke incidence is about 200 per 100,000 people throughout the world. This study aims to determine the Relation Suffer Stroke With Independence Level In Neurology Polyclinic TK II DR Ak Gani Palembang Year Hospital 2017. The research method used is descriptive quantitative with cross sectional design that is done by interviewing techniques with questionnaires on 42 respondents with Accidental sampling technique. This research was conducted in August 2017. Data analysis used is univariate data analysis and bivariate data analysis with one way anova test result. The results of univariate analysis showed that the duration of the respondents suffering from stroke was between 2.10 years to 3.38 years. Also found that most respondents were at the level of independence f; independent, except bathing, dressing, moving, and one other function with a total of 12 respondents. The results showed that there was a significant relationship between the long suffering stroke with the level of independence with the value of p value 0.025. For that the need for rehabilitation to patients and families of patients in order to help improve the independence of stroke patients in doing their daily activities. Keywords : Long Suffer Stroke, Level of Independence

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