scholarly journals Effect of a Fungi Complex in Nine Ecotypes of Cenchrus purpureus (Schumach) Morrone

2021 ◽  
Vol 13 (12) ◽  
Author(s):  
Primavera Pérez-Ramos ◽  
Yuri Villegas-Aparicio ◽  
Rigoberto Castro-Rivera ◽  
Ernesto Castañeda-Hidalgo ◽  
Aarón Martínez-Gutiérrez ◽  
...  
Keyword(s):  
Differential Effect ◽  
Leaf Length ◽  
Growth And Yield ◽  
Total Biomass ◽  
Growth Strategies ◽  
Randomized Design ◽  
Fungal Consortium ◽  
Number Of Leaves ◽  
Completely Randomized Design ◽  
Four Levels

Objective: The objective of this study was to evaluate the response of different concentrations of a fungal consortium on the growth and yield of Cenchrus purpureus ecotypes under storm conditions.Design/Methodology/Approach: An experiment was established under a completely randomized design with a 9X2X4 factorial arrangement. The factors were 9 ecotypes of Cenchrus purpureus, two seasons of the year (Summer-Fall and Winter-Spring), and four levels of mycorrhizal consortium. The variables evaluated were: number of buds, number of leaves, height of the bud, leaf length, total biomass, and leaf-stalk ratio.Results: The response of the ecotypes had a differential effect on the variables NB, LLL, TB and L/SR, while the season affected the variables LN, PH, LLL, MLL, SLL, TB and L/SR. Low inoculation levels increased the variables NB, PH, LLL, MLL and TB.Study Limitations/Implications: The evaluated factors indicate that the level of inoculant and the season determine the growth and yield of Cenchrus purpureus.Findings/Conclusions: The mycorrhizal consortium dose and season of the year mainly determined the growth and yield of C. purpureus. Ecotypes respond differently to changes in the season and in mycorrhizal consortium dose. The evaluated factors indicate that the mycorrhiza dose and the season of the year determine the growth and yield of C.purpureus. Two growth strategies of the ecotypes are visualized: 1) many buds, with few small leaves and 2) few buds with many large leaves

scholarly journals Perlakuan Berbagai Dosis Pupuk ZA Pertumbuhan Dan Hasil Tanaman Bayam Merah (Amaranthus gangeticus)

2021 ◽  
Vol 5 (2) ◽  
pp. 180
Author(s):  
Edy Kustiani ◽  
Mariyono Mariyono ◽  
Bela Citra Ayuningtyas
Keyword(s):  
Analysis Of Variance ◽  
Nutritional Value ◽  
Environmental Design ◽  
Dry Weight ◽  
Growth And Yield ◽  
Dose Treatment ◽  
Randomized Design ◽  
Number Of Leaves ◽  
Completely Randomized Design ◽  
The Ideal

Red spinach (Amaranthus gangeticus) is one of the most popular vegetables because of its high nutritional value and easy cultivation. Efforts to increase the production of red spinach are to fertilize to meet the needs of both macro and micronutrients. This study aimed to determine the effect of ZA fertilizer dose on the growth and yield of red spinach. The environmental design used was a non-factorial, completely randomized design with four replications. The factors tested were the dose of ZA fertilizer which consisted of 6 levels, namely: without ZA fertilizer (D0); 0.4 gr/polybag (D1); 0.8 gr/polybag (D2); 1.2 gr/polybag (D3); 1.6 gr/polybag (D4); 2 gr/polybag (D5). The data obtained were analyzed using analysis of variance, and if the results were significantly different, the BNT test was continued at a 5% level. The growth and yield of red spinach showed significant differences due to the ZA fertilizer dose treatment. The ZA fertilizer dose treatment of 1.2 g/polybag (D3) resulted in the highest plant height, the number of leaves, and leaf area of red spinach at 14, 21, and 28 days after planting. The ZA fertilizer dose treatment of 1.2 g/polybag (D3) resulted in the highest wet and dry weight plants of 49.15 and 4.38 grams, respectively. The ZA 1.2 gr/polybag dose is the ideal dose to increase the productivity of red spinach.Bayam merah (Amaranthus gangeticus) termasuk sayuran penting yang disukai masyarakat karena bernilai gizi tinggi dan mudah pembudidayaannya. Upaya peningkatan hasil bayam merah salah satunya dengan pemupukan untuk menambah nutrisi yang dibutuhkan tanaman. Tujuan dari penelitian ini adalah mempelajari respon pertumbuhan dan hasil tanaman bayam merah pada perlakuan dosis pupuk ZA. Pelaksanaan penelitian ini menggunakan Rancangan Acak Lengkap (RAL) satu faktor. Faktor yang diujikan adalah dosis pupuk ZA, terdiri 6 taraf, yaitu: tanpa pupuk ZA (D0); 0.4 gr/polybag (D1); 0.8 gr/polybag (D2); 1.2 gr/polybag (D3); 1.6 gr/polybag (D4); 2 gr /polybag (D5). Percobaan dilakukan dengan ulangan sebanyak 4 kali. Analisis data menggunakan analysis of variance (ANOVA), apabila berbeda nyata dilanjutkan uji Beda Nyata Terkecil (BNT) taraf 5%. Perlakuan dosis pupuk ZA berpengaruh nyata terhadap pertumbuhan dan hasil tanaman bayam merah. Perlakuan dosis pupuk ZA 1.2 gr/polybag (D3) menghasilkan tinggi, jumlah dan luas daun tanaman bayam merah tertinggi pada pengamatan umur 14, 21, dan 28 HST. Dosis pupuk ZA 1.2 gr/polybag (D3) juga menunjukkan berat basah dan berat kering tanaman tertinggi sebesar 49.15 dan 4.38 gram. Dosis ZA 1.2 gr/polybag merupakan dosis ideal untuk meningkatkan produktivitas tanaman bayam merah.

scholarly journals In vitro development of Encyclea cordigera in different concentrations of honey

2015 ◽  
Vol 46 (4) ◽  
pp. 590-592 ◽  
Author(s):  
Cibele Mantovani ◽  
Kathia Fernandes Lopes Pivetta
Keyword(s):  
Culture Media ◽  
Leaf Length ◽  
In Vitro Development ◽  
Randomized Design ◽  
Sucrose Medium ◽  
Number Of Leaves ◽  
Completely Randomized Design ◽  
Set Up ◽  
Vitro Development

ABSTRACT: The objective of this paper was to evaluate the effects of different honey concentrations in culture media, in comparison to sucrose medium, for the in vitro development of the epiphytic Encyclea cordigera orchid, in order to improve the process of propagation of the species. The in vitro germination was prepared on a reduced Murashige & Skoog (MS) medium. After 90 days, the seedlings were divided into different treatments, where they remained for another 90 days. Six treatments were set up (30g L-1 of sucrose; 15, 30, 45, and 60g L-1 of honey; and absence of any carbohydrates) in a completely randomized design. Plants were removed from the vials 270 days after the start of the experiment, and the number of roots, length of the largest leaf, length of the longest root, number of leaves, and fresh and dry masses were evaluated. Data concerning the number of leaves and roots were (x+1)1/2 transformed and subjected to an analysis of variance (ANOVA); the means were compared by a Tukey's test set at 5% probability. Medium containing 60g L-1 of honey proved to be superior to the sucrose medium traditionally used, favoring the in vitro growth and development of Encyclea cordigera. This medium can therefore be recommended for the propagation of this species, which is usually cultivated as an ornamental plant.

scholarly journals Utilização de diferentes substratos na propagação de Pyrostegia venusta através de estacas

2015 ◽  
Vol 10 (4) ◽  
pp. 38
Author(s):  
J. G. Crispim ◽  
M. M. Rêgo ◽  
E. R. Rêgo ◽  
Â. M. S. Pessoa ◽  
P. A. Barroso
Keyword(s):  
Analysis Of Variance ◽  
Leaf Length ◽  
Cattle Manure ◽  
Main Root ◽  
Plastic Tube ◽  
Tukey Test ◽  
Randomized Design ◽  
Number Of Leaves ◽  
Completely Randomized Design ◽  
Pyrostegia Venusta

<p>Este trabalho teve por objetivo avaliar o desenvolvimento de clones de <em>Pyrostegia venusta </em>em diferentes tipos de substratos. O experimento foi conduzido em delineamento inteiramente casualizado, com 06 tratamentos e 10 repetições, cada repetição foi constituída por um tubete de polietileno com capacidade de 103cm<sup>3</sup>. Os tratamentos testados foram: (T1) 100% de areia lavada; (T2) 50% areia lavada + 50% substrato comercial Plantmax®; (T3) 100% de substrato comercial Plantmax®; (T4) 50% areia lavada + 50% de esterco bovino; (T5) 1/3 areia lavada + 1/3 esterco bovino +1/3 de Plantmax® e (T6) 50% esterco bovino + 50% de Areia lavada. Após 30 dias foram avaliados o número de estacas sobreviventes, comprimento do broto (cm), número de folhas, comprimento da folha (cm), número de raízes e comprimento da raiz principal (cm).  Os dados foram submetidos a análise de variância e as médias comparadas pelo teste de Tukey a 5% de probabilidade. Por meio da análise de variância (p&lt;0,01), detectou-se significância estatística, para as variáveis, sobrevivência das estacas e número de brotos por estaca de <em>P. venusta</em>, quando cultivadas em substrato composto por 50% areia lavada + 50% substrato comercial Plantmax®. Essa mistura apresenta a vantagem de ser mais econômica quando comparado ao substrato comercial Plantmax®, sendo portanto, recomendada na produção de mudas de <em>cipó-de-São João</em><em>.</em></p><p align="center"><strong><em>Utilization of different substrates in the propagation of Pyrostegia venusta by cuttings</em></strong></p><p><strong>Abstract</strong><strong>: </strong>The experiment was carried out in a completely randomized design with 06 treatments and 10 replicates, each replicate consisted of polyethylene plastic tube the size of 103cm<sup>3</sup>. The treatments were: (T1) 100% washed sand; (T2) 50% washed sand + 50% commercial substrate Plantmax®; (T3) 100% substrate commercial Plantmax®; (T4) 50 % washed sand + 50% cattle manure; (T5) 1/3 washed sand + 1/3 manure +1/3 Plantmax® and Plantmax (T6) 50 % cattle manure + 50% washed sand. After 30 days, were evaluated number of cutting survival, bud length in cm, number of leaves, leaf length in cm, number of roots and length of the main root. Data were submitted to analysis of variance and means were compared by Tukey test at 5% probability. By analysis of variance (p&lt; 0.01), there was significant differences for the variables, czutting survival and number of shoots per cutting when cultured on substrate composed of 50% washed sand + 50% commercial substrate Plantmax®. Furthermore, this mixture has the advantage to be cheaper as compared to substrate Plantmax®, so we recommend the seedlings of Saint John’s vine.</p><p><em><br /></em></p>

scholarly journals EFEK KONSENTRASI DAN WAKTU APLIKASI PUPUK ORGANIK CAIR KULIT PISANG TERHADAP PERTUMBUHAN DAN HASIL TANAMAN KUMIS KUCING (Orthosiphon aristatus)

2020 ◽  
Vol 22 (1) ◽  
pp. 31-37
Author(s):  
Detri Saputra ◽  
Entang Inoriah Sukarjo ◽  
Masdar Masdar
Keyword(s):  
Organic Fertilizer ◽  
High Growth ◽  
Growth And Yield ◽  
Application Time ◽  
Plant Weight ◽  
Randomized Design ◽  
Number Of Leaves ◽  
Tea Production ◽  
Completely Randomized Design ◽  
The City

[EFFECT OF CONCENTRATION AND APPLICATION TIME OF ORGANIC FERTILIZER LIQUID BANANA PEELS ON GROWTH AND YIELD OF JAVA TEA (Orthosiphon aristatus)]. Java tea are medicinal plants that have many health benefits but java tea production is very low. Efforts are made to increase the growth and yield of java tea, namely the use of liquid organic fertilizer (LOF) banana peels. This study aims to obtain concentration, application time of LOF banana peels, and interactions between the two that produce high growth and yield of java tea. The study was conducted from November 2018 to February 2019 in the city of Bengkulu. The experiments were arranged based on a completely randomized design factorial pattern. The first factor is the LOF concentration of banana peels 25 mL/L, 50 mL/L, 75 mL/L, and 100 mL/L. The second factor is the time of LOF application which consists of 1 week application, 2 weeks application, and 3 weeks application. The results showed that independently giving concentration and application time and interaction did not significantly influence the variable thickness of leaves, total leaf area, shoot length, number of leaves, fresh plant weight, root length, and dry plant weight.

scholarly journals Respon Pertumbuhan Tanaman Gelombang Cinta Akibat Pemberian Pupuk Daun Petrovita di Pembibitan Lanjutan

2013 ◽  
Vol 13 (1) ◽  
pp. 10-18
Author(s):  
Djoko Eko Hadi Susilo ◽  
Sri Handayani Wahyuningsih
Keyword(s):  
Love Wave ◽  
Growth Response ◽  
Leaf Length ◽  
Stem Height ◽  
Efficient Treatment ◽  
Randomized Design ◽  
Number Of Leaves ◽  
Completely Randomized Design ◽  
Leaf Fertilizer ◽  
Treatment Design

This study aims to determine the growth response of the love wave plant (Anthurium plowmanii) on Petrovita leaf fertilizer in an advanced nursery conducted in August - October 2011 in Panarung Village, Pahandut District, Palangka Raya City. Single treatment design (one factor) with 10 (ten) repetitions in the form of Petrovita leaf fertilizer (D) consisting of 5 concentration levels, namely: d1 = 0.5 ml l-1 (0.05%); d2 = 1.0 ml l-1 (0.10%); d3 = 1.5 ml l-1 (0.15%); d4 = 2.0 ml l-1 (0.20%); and d5 = 2.5 ml l-1 (0.25%) using a completely randomized design. Observations were made when the plants in the advanced nurseries had received treatment applications 4 times at intervals of 2 weeks. Observations were made on plant height (cm); stem height (cm); number of leaves (leaves); leaf length (cm); leaf width (cm); and leaf area (cm2). The results of this study indicate that Petrovita complete leaf fertilizer has a very significant effect on increasing the growth of love wave plants aged 8 MST in advanced nurseries. Petrovita fertilizer concentration of 0.2% or 2 ml l-1 (d4) is the best and most efficient treatment for love wave plants in advanced nurseries. It is recommended to increase the growth of love wave plants in advanced nurseries using Petrovita 0.2% concentration but tried with an interval of less than 2 weeks (for example once a week).

scholarly journals Leek (Allium fistulosum, L.) Growth and Yield as Affected by Cow Manure and Guava Waste Liquid Organic Fertilizer

2021 ◽  
Vol 4 (3) ◽  
pp. 305-313
Author(s):  
Nanik Setyowati ◽  
Nova Hardianto ◽  
Widodo Widodo ◽  
Zainal Muktamar
Keyword(s):  
Organic Fertilizer ◽  
Experimental Treatment ◽  
Growth And Yield ◽  
Allium Fistulosum ◽  
Cow Manure ◽  
Fresh Weight ◽  
Good Source ◽  
Randomized Design ◽  
Number Of Leaves ◽  
Completely Randomized Design

Fruit waste can be a good source of liquid organic fertilizer (LOF) because it contains macro and micronutrients needed by plants. This study aims to determine the type and concentration of LOF for the growth and yield of leek (Allium fistulosum, L.). This research was conducted from June to August 2019, in Pematang Gubernur, Muara Bangkahulu, Bengkulu City, Indonesia. Treatment consist of types of Liquid Organic Fertilizer (LOF) which were cow manure (CM) and guava waste (GW) at concentrations of 25%, 50%, and 75%. Experimental treatment included; without LOF, CM LOF (25%), CM LOF (50%), CM LOF (75%), GW LOF (25%), GW LOF (50%), GW LOF (75%), CM LOF + GW LOF (25%), CM LOF + GW LOF (50%) and CM LOF + GW LOF (75%). The study used a completely randomized design (CRD), one factor, repeated five times. Differences in LOF concentration and source significantly affected the number of leaves and tiller diameter. Nonetheless, it did not affect the number of tillers, plant height, and fresh weight. Cow manure liquid organic fertilizer at a 50 % concentration raised the number of leaves by 35 %, whereas guava waste increased the leaves numbers by 25%. With the application of guava waste liquid organic fertilizer at a concentration of 75%, so the number of tillers increased by 23%.

scholarly journals UJI BERBAGAI JENIS MIKROORGANISME LOKAL (MOL) TERHADAP PERTUMBUHAN DAN HASIL TANAMAN PAKCHOY (Brassica Rapa L) SECARA HIDROPONIK

2020 ◽  
Vol 3 (1) ◽  
Author(s):  
Tony Tony ◽  
Setiawan Setiawan ◽  
Rahmawaty Rahman ◽  
Yulianti Rasud
Keyword(s):  
Leaf Length ◽  
Plant Diseases ◽  
Growth And Yield ◽  
Banana Weevil ◽  
Fresh Weight ◽  
Local Resources ◽  
Randomized Design ◽  
Number Of Leaves ◽  
Organic Pesticides ◽  
Biological Fertilizer

ABSTRACTLocal microorganisms (MOL) are fermented solutions made from various local resources. MOL solution containsmicro and macro nutrients as well as containing bacteria that have the potential to remodel organic matter,stimulate growth, and as a controlling agent for pests and plant diseases, so that MOL can be used both as adecomposer, biological fertilizer and organic pesticides, especially as a fungicide. The aim of this experiment wasto determine the most suitable MOL types of the growth and yield of pakchoy plants. This experiment used aCompletely Randomized Design (CRD) with treatments to be tried, namely various types of MOL at aconcentration of 10 ml. L-1 consisting of 5 treatments, namely B1 = Control (without MOL), B2 = MOL Rice,B3 = MOL Vegetables , B4 = MOL Banana weevil, B5 = MOL Banana skin. The results showed that theadministration of rice mole was a better treatment of plant height, number of leaves, leaf length, leaf width untilthe fourth week of 25.94 cm each; 19.98 strands; 9.59 cm; 5.71 cm; and the fresh weight of pakcoy plants is 68.48g.Keyword : Local Mikroorganisms, Pakchoy, HidroponicABSTRAKMikroorganisme Lokal (MOL) adalah larutan hasil fermentasi yang berbahan dasar dari berbagai sumber dayalokal. Larutan MOL mengandung unsur hara mikro dan makro serta mengandung bakteriyang berpotensi sebagaiperombak bahan organik, perangsang pertumbuhan, dan sebagai agen pengendali hama dan penyakit tanaman,sehingga MOL dapat digunakan baik sebagai dekomposer, pupuk hayati maupun pestisida organik terutamasebagai fungisida.Penelitian ini bertujuan untuk untuk menentukan jenis MOL yang lebih baik terhadappertumbuhan dan hasil tanaman pakcoy. Penelitian ini menggunakan Rancangan Acak Lengkap (RAL) denganperlakuan yang akan dicobakan yaitu berbagai jenis MOL pada konsentrasi 10 ml.L-1 yang terdiri dari 5perlakuan, yaitu B1 = Kontrol (Tanpa MOL), B2 = MOL Nasi , B3 = MOL Sayuran, B4 = MOL Bonggol Pisang,B5 = MOL Kulit Pisang. Data diolah dengan analisis ragam dan perbedaan antar perlakuan ditentukan dengan UjiBNJ 5%. Hasil penelitian menunjukkan pemberian mol nasi merupakan perlakuan yang lebih baik terhadap tinggitanaman, jumlah daun, panjang daun, lebar daun hingga minggu keempat yaitu masing-masing 25,94 cm ; 19,98helai; 9,59 cm; 5,71 cm; dan berat segar tanaman pakcoy yaitu 68,48 g.Kata Kunci : Mikroorganisme Lokal, Pakchoy, Hidroponik

scholarly journals The Results of Testing Several Potato Varieties (Solanum tuberosum L.) Under The Stands of Immature Coffee Plants

2020 ◽  
Vol 3 (1) ◽  
pp. 12-15
Author(s):  
Benni Satria
Keyword(s):  
Production Systems ◽  
Cropping Systems ◽  
Solanum Tuberosum L ◽  
Leaf Length ◽  
Growth And Yield ◽  
Multiple Cropping ◽  
Potato Varieties ◽  
Best Response ◽  
Number Of Leaves ◽  
Four Levels

Potatoes have the potential to be developed as a source of carbohydrates in supporting food diversification programs. Reduced potatoes production has resulted from a decrease in the land area for potato cultivation. The effort that can be made to increase land productivity is through multiple cropping systems such as intercropping, intermittent intercropping, streak planting, annual intercropping, and agroforestry. It is necessary to use idle land under plantation stands that have not been optimized, in increasing productivity and efficiency of agricultural production systems. This study aims to get the varieties that have the best growth and yields planted under immature coffee stands. This research applies a Single Factorial Experiment in Completely Random Design, where the factor is four levels of potato varieties, Granola (V1), Bliss (V2), Kentang Merah (V3) and Batang Hitam (V4). Observations made include plant height, number of leaves, leaf length, leaf width, tuber length, tuber diameter, weight per tuber, number of tubers/plat, and total tuber by grade tubers (Small, Medium, Large, Very large). Based on the analysis variance of each observation variable shows, there is an effect of the variable observation except the width of the leaves. Granola provides the best response to the growth and yield of potato plants

scholarly journals PENGARUH KOMBINASI PUPUK ORGANIK DAN PUPUK HAYATI TERHADAP PERTUMBUHAN, PRODUKSI DAN MUTU PEGAGAN

2021 ◽  
Vol 32 (1) ◽  
pp. 23
Author(s):  
Rahma Widyastuti ◽  
Nurul Husniyati Listyana ◽  
Erri Setyo Hartanto
Keyword(s):  
Dry Weight ◽  
Growth And Yield ◽  
Stem Length ◽  
Fresh Weight ◽  
Chemical Fertilizers ◽  
Cultivation Method ◽  
Organic Cultivation ◽  
Randomized Design ◽  
Number Of Leaves ◽  
Completely Randomized Design

<em>Gotu kola is one of the medicinal plants that has begun to be cultivated by the Indonesian people. It’s cultivation needs to follow the GAP to produce the qualified yield, including the use of fertilizers. Biofertilizers have been widely developed to reduce adverse effects of chemical fertilizers to the environment, thus an organic cultivation method can be an alternative. The aim of this study was to determine the effect of biofertilizer on growth and yield of gotu kola. This research was an experimental study with a completely randomized design (CRD) using 8</em> <em>planting media treatments consisting of (a) Soil + manure + dolomite; (b) Soil manure + 1 g biofertilizer; (c) Soil + dolomite + 1g biofertilizer; (d) Soil + manure + 3 biofertilizer; (e) Soil + dolomite + 3 biofertilizer; (f) Soil + manure + 5 g biofertilizer; (g)  Soil + dolomite + 5 g  biofertilizer;  and  (h)  Soil +  3 g</em> <em>biofertilizer. Parameters observed were leaf area, stem length, number of leaves, number of tillers, fresh weight, dry weight and asiaticoside content. The results showed that combination treatment of biofertilizer (1 g) with dolomite (30 g) gives better effect in increasing the number of tillers and number of leaves, while the combination of biofertilizer (5 g) and dolomite (30 g) is the best for asiaticoside levels in gotu cola.</em>

scholarly journals Peran Chitosan sebagai Pemacu Pertumbuhan Kultur Anggrek Dendrobium lasianthera J.J.Sm. secara in vitro

2020 ◽  
Vol 12 (1) ◽  
pp. 43-49
Author(s):  
Verena Agustini ◽  
Irma Rahayu ◽  
Leonardo A. Numberi ◽  
Ziyadatun Ni’mah
Keyword(s):  
Positive Influence ◽  
Leaf Length ◽  
Maximum Effect ◽  
Observation Data ◽  
Chitosan Concentration ◽  
Randomized Design ◽  
The Media ◽  
Number Of Leaves ◽  
Completely Randomized Design

The effect of chitosan  on micropropagation of Dendrobium lasianthera J.J.Sm was observed. The media used was Vacin and Went (VW) modified with the addition of coconut water and given chitosan with several concentrations. The study was conducted at the Plant Tissue Culture Laboratory of the Department of Biology, Faculty of Mathematics and Natural Sciences, Cenderawasih University for 6 months, from April to September 2019. Experiments using a Completely Randomized Design (CRD) of 8 treatments with 3 explants in each bottle for total 3 bottle as a replication. The total explants used in this experiment was 72 explants. The concentration of chitosan were 0% , 1%, 5%, 10%, 15%, 20%, 40% and 50% separately. Observation data were analyzed using ANOVA, if there were significant differences followed by DMRT at the 5% level. Observation variables included number and length of roots, number and length of leaves. The results showed that giving chitosan 10% had a maximum effect on the number of roots and number of leaves, 6.67 and 4.67. As for the highest leaf length of 17.43 mm  and the longest root length 19.21 mm were in the media with the addition of chitosan concentration of 15%. The observations showed that chitosan  had a positive influence on the growth and development of orchids D. lasianthera plantlet  in vitro.Keywords : Chitosan, orchid, Vacin and Went, plantlet.

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