scholarly journals PURPLE SWEET POTATO (Ipomoea batatas L.) PEELS EXTRACT AS AN ALTERNATIVE DYE FOR BACTERIA GRAM STAINING

2020 ◽  
Vol 2 (2) ◽  
pp. 76-84
Author(s):  
Nastasya Nunki ◽  
Diah Titik Mutiarawati ◽  
Endah Prayekti
Keyword(s):  
Sweet Potato ◽  
Ipomoea Batatas ◽  
Gentian Violet ◽  
Glass Slide ◽  
Control Group ◽  
Anthocyanin Pigment ◽  
Extract Concentration ◽  
Gram Staining ◽  
Potato Peels ◽  
Purple Sweet Potato

Crystal violet and Safranin are dyes in Gram staining, which are carcinogenic. Alternative safe materials are needed to minimize the use of carcinogenic properties. Purple sweet potato (Ipomoea batatas L.) peels were the candidate of the alternative dye source because of its high anthocyanin pigment. The purpose of this study was to determine purple sweet potato (Ipomoea batatas L.) peels extract as an alternative to Gentian violet in Gram staining of bacteria. Extracts obtained from purple sweet potato peels studied with varying concentrations of 50%, 60%, and 75% for 1, 3, and 5 min as a substitute for Gentian violet on Bacillus sp. The parameters observed from this study based on visual field clarity, glass slide cleanliness, contrast, bacterial shape, bacterial colour. Each extract concentration compared with a control group using Gentian violet. The results showed that optimum staining in 50% concentration for 5 min, 60% concentration for 5 min, 75% concentration for 3 min, and 5 min respectively. The present study exhibited the potency of Ipomoea batatas L. peels extract as an alternative staining agent.

Pengaruh Profilaksis Ekstrak Ubi Jalar Ungu (Ipomoea batatas L.) terhadap Kadar Malondialdehida Hepar Tikus Putih (Rattus norvegicus) Jantan Galur Wistar yang Diinduksi Karagenan

2018 ◽  
Vol 15 (2) ◽  
pp. 146
Author(s):  
BRILIAN DINANTI ◽  
FITRI HANDAJANI
Keyword(s):  
Sweet Potato ◽  
Ipomoea Batatas ◽  
Wistar Rats ◽  
Group Method ◽  
Control Group ◽  
Intraplantar Injection ◽  
Significant Difference ◽  
Male Wistar Rats ◽  
Potato Extract ◽  
Purple Sweet Potato

<p>Liver is an organ with complex metabolism. When the liver is inflamed, cellular immunity will defend against inflammatory agents by stimulating immune cells to produce reactive oxygen species (ROS). Excessive ROS accumulation cause oxydative stress with increased  liver malondialdehyde (MDA) level. Some researches showed that purple sweet potato contain flavonoids (anthocyanins) that functioned as antioxydants. This study aimed to show the prophylactic effect of purple sweet potato extract to the liver MDA level of male Wistar rats induced by carrageenan.</p><p>This study used post-only control group method using 18 male Wistar rats divided into 3 groups: group of rats without treatment, group of rats induced by 0,1 ml of 1% carrageenan by intraplantar injection on day-8, and group of rats given with 872 mg/kgBW of purple sweet potato extract for 7 days and induced by 0,1 ml of 1% carrageenan. In the end of the study, the liver MDA levels were measured by Thio-Barbituric Acid method on each groups.</p><p>The results of One-Way ANOVA test showed there was no significant difference (p = 0,290) between group of rats without treatment (<em>x̅</em>= 207,50) and group of rats induced by carrageenan (<em>x̅</em>=233,17). Then, there is no significant difference (p = 0.978) between group of rats induced by carrageenan and group of rats given with prophylactic purple sweet potato extract and induced by carrageenan (<em>x̅</em>= 232,50).</p><p>The conclusion of this study is giving intraplantar injection of carrageenan can increase liver MDA level insignificantly and giving prophylactic purple sweet potato extract has an effect to decrease the liver MDA level of rats induced by carragenan insignificantly because it contains anthocyanins as antioxidants.</p><p> </p><strong>Keywords: </strong>Liver, <em>Ipomoea batatas</em> L., Malondialdehyde, Anthocyanins

scholarly journals Effect of purple sweet potato (Ipomoea batatas L) extract on malondialdehyde levels of male white rat (Rattus norvegicus Wistar strain) model of atherosclerosis

2019 ◽  
Author(s):  
Meddy Setiawan ◽  
Fakih Nadhil
Keyword(s):  
Sweet Potato ◽  
Rattus Norvegicus ◽  
Ipomoea Batatas ◽  
Control Treatment ◽  
Control Group ◽  
Experimental Animals ◽  
Wistar Strain ◽  
Potato Extract ◽  
Purple Sweet Potato ◽  
Strain Model

Cardiovascular disease is a disease caused by impaired functions of heart and blood vessel, one of those is coronary heart disease. This study aimed to identify the effect of purple sweet potato (Ipomoea batatas L) extract on MDA (Malondialdehyde) levels of male white rat (Rattus norvegicus Wistar strain) model of atherosclerosis. This research was an experimental study using Post-Test Only Control Group Design method, used 5 groups (positive control group, negative control, treatment 1, treatment 2, and treatment 3), each group contained 5 rats plus 2 reserve rats. ANOVA one way test obtained a significance of 0.00 (p <0.05). The regression test results showed the coefficient of determination R² = 0.71, which means a decrease in serum MDA levels of experimental animals is influenced by the dose of purple sweet potato extract administration as much as 71% while the remaining 29% can be influenced by factors outside the study. It can be concluded that purple sweet potato extract can affect serum MDA levels of experimental animals.

The Effect of Fermented Purple Sweet Potato (Ipomoea batatas L) Skin in Diets on Feed and Anthocyanin Consumption, Carcass Characteristics, Anthioxidant Profile and Meat Texture of Bali Duck

2016 ◽  
Vol 2 (9) ◽  
pp. 57
Author(s):  
T.G. Belawa Yadnya ◽  
AA. A.S. Trisnadewi ◽  
I Ketut Sukada ◽  
I Gusti Lanang Oka
Keyword(s):  
Superoxide Dismutase ◽  
Sweet Potato ◽  
Ipomoea Batatas ◽  
Control Diet ◽  
Carcass Characteristics ◽  
Carcass Weight ◽  
Control Group ◽  
Antioxidant Profile ◽  
Potato Skin ◽  
Purple Sweet Potato

An experiment was carried out to determine the effect of fermented purple sweet potato (Ipomoea batatas L) skin in diets on feed and anthocyanin consumption, carcass characteristics antioxidant profile and meat texture of Bali duck.. Five  treatment diets were used in a completely randomized design (CRD) consisted of control diet A (diet without containing purple sweet potato skin), diet B containing 10% purple sweet potato skin, diet C containing 10% fermented purple sweet potato skin, diet D containing 20% purple sweet potato skin, diet E containing 20% fermented purple sweet potato skin.Each treatment consisted of four replications and each replicate had five ducks. The variables observed were feed and anthocyanin consumption,slaughter weight, carcass weight, carcass percentage, carcass physical composition,  meat antioxidant profile (capacity antioxidant (CA), malondialdehide (MDA), and superoxide dismutase (SOD). Experiment results showed that diets with fermented or unfermented purple sweet potatoin diets treatments B, C, D, and E increased the anthocyanin  consumption,  slaughter weight, carcass weight, carcass percentage and meat percentage of duck carcass but decreased  their fat and skin. Fermented purple sweet potato (Ipomoea batatas L) skin  in  C and E increased the antioxidant capacity(AC)  and superoxide dismutase (SOD) while  treatment B and  D were not significantly different (P>0.05) to the control group.  Malondialdehide (MDA) of the duck meat in treatment B,C, and D were significantly lower (P<0.05) compared to the control diet. Treatment E significantly  increased the perimesium and endomesium of meat texture , but its experiment was not significantly different to the control group. It was concluded that substitution of fermented purple sweet potato (Ipomoea batatas L) skin in diets could improve of feed and anthocyanin, carcass characteristics, antioxidant profile, and meat texture of bali duck.

scholarly journals UMBI UBI JALAR UNGU BALI (Ipomoea batatas) DI TRANSAMINASE SERUM, MALONDIALDEHIDE HEPAR DAN ALKOHOL KRONIS

2018 ◽  
Vol 17 (3) ◽  
pp. 151
Author(s):  
I Wayan Putu Sutirta-Yasa ◽  
I Made Jawi ◽  
Ida Bagus Ngurah ◽  
Anak Agung Ngurah Subawa
Keyword(s):  
Sweet Potato ◽  
Liver Tissue ◽  
Ipomoea Batatas ◽  
Ethanol Extract ◽  
Antioxidant Effect ◽  
Control Group ◽  
Chronic Alcoholic ◽  
Purple Sweet Potato ◽  
Group Post ◽  
Alcoholic Intake

Oxidative stress as a consequence of chronic alcoholic intake causes lesions of liver tissue. The objective of this study was to identifythe antioxidant effect of ethanol extract derived from Balinese purple sweet potato on the liver tissue in mice after chronic consumptionof alcohol. Subjects of this study were 32 adults male Swiss mice (12–14 weeks) divided into 4 groups with control group post-testonly design. Post-treatment of blood and liver samples were collected from each group of 8 mice. The MDA liver was quantified withthiobarbituric acid reactive substances (TBARS) method. Samples were collected from control group of 8 mice without sweet potatoextract and alcohol, 8 mice with 1 mg extract of Balinese purple sweet potato, 8 mice with 0.8 gram alcohol, and 8 mice with combineof alcohol and extract of Balinese purple sweet potato, each treatment was carried out every day for 4 weeks. The results showed asignificant increase of serum SGOT, SGPT and liver MDA level, after chronic consumption of alcohol (p = 0.00). The decrease of serumSGOT, SGPT and liver level MDA was significant in the group with extract of Balinese purple sweet potato (p = 0.00). The researchersconcluded that the extract of Balinese purple sweet potato had an antioxidant effect in mice which consumed alcohol chronically. Basedon this study it is concluded that extract of Balinese purple sweet potato has a protective effect on the liver cell in mice which consumedalcohol chronically.

scholarly journals Neuroprotective Effects of Purple Sweet Potato Balinese Cultivar in Wistar Rats With Ischemic Stroke

2018 ◽  
Vol 6 (11) ◽  
pp. 1959-1964 ◽  
Author(s):  
I Made Oka Adnyana ◽  
AA Raka Sudewi ◽  
DPG Purwa Samatra ◽  
DN Suprapta
Keyword(s):  
Ischemic Stroke ◽  
Cytochrome C ◽  
Sweet Potato ◽  
Ipomoea Batatas ◽  
Caspase 3 ◽  
Neuroprotective Effect ◽  
Control Group ◽  
Neuroprotective Effects ◽  
Apoptosis Rate ◽  
Purple Sweet Potato

BACKGROUND: Purple sweet potato (Ipomoea Batatas L.) is one of the sources for anthocyanin, which promotes the health through antioxidant, anti-inflammatory, anti-cancer, neuroprotection, and anti-apoptosis activities. Oxidative stress has been shown to be the cause of apoptosis in ischemic stroke. AIM: The objective of this research was to delineate the pleiotropic effects of anthocyanin for neuroprotection during an acute stroke event. METHODS: Anthocyanin was extracted from Balinese cultivar of purple sweet potato and subsequently administered to rat models of induced ischemic stroke (labelled as treatment group), as well as a placebo (labelled as a control group). Several parameters were in turn evaluated, i.e. the activities of anti-apoptotic (Bcl-2) as well as pro-apoptotic (cytochrome c, caspase-3) molecules, and apoptosis rate. Bcl-2 levels were determined using the histochemical method, cytochrome c and caspase-3 via ELISA method, while apoptosis rate was measured by TdT-medicated Dutp-Nick End Labeling (TUNEL) assay. RESULTS: Bcl-2 expression demonstrated significantly higher Bcl-2 expression in the treatment compared with control group (median 31.2 vs. 1.1; p = 0.001). Accordingly, pro-apoptotic cytochrome c and caspase-3 levels were also found significantly lower in the treatment as opposed to control group (mean 4.17 vs. 8.06; p = 0.001; mean 3.81 vs. 8.02; p = 0.001). Ultimately, apoptosis rate was found markedly lower among treatment than control groups (mean 3.81 vs. control 21.97; p = 0.003). CONCLUSION: The results of this study indicated a significant neuroprotective effect of anthocyanin derived from Balinese cultivar of PSP. Anthocyanin was able to increase and reduce anti-apoptotic and pro-apoptotic protein levels, respectively, resulting in lesser cellular apoptotic rate when compared with placebo. The potential mechanism was thought mainly due to its anti-oxidant properties.

scholarly journals Pengaruh Pemberian Ekstrak Etanol Daun Ubi Jalar Ungu (Ipomoea Batatas (L.) Lam) terhadap Kadar Kolesterol Ldl Tikus Hiperkolesterolemia

2019 ◽  
Vol 2 (2) ◽  
pp. 184
Author(s):  
Siti Fatimah ◽  
Yuliana Prasetyaningsih
Keyword(s):  
Treatment Group ◽  
Cholesterol Level ◽  
Sweet Potato ◽  
Ipomoea Batatas ◽  
Ldl Cholesterol ◽  
Ethanol Extract ◽  
Control Group ◽  
Cholesterol Levels ◽  
Purple Sweet Potato ◽  
Sweet Potato Leaves

Hypercholesterolemia is still a health problem today because it is associated with the onset of cardiovascular disorders with various complications. High cholesterol levels carried by the blood will accumulate in the arteries that cause atherosclerosis. Blood clots and blockage of blood vessels can result in the stroke or heart attack. Flavonoids that are found in purple sweet potato leaves are quercetin. Quercetin has antioxidant effects that can improve blood vessel endothelial function, reduce LDL sensitivity to free radical effects, and reduce blood lipid levels. This study aims to determine the effect of ethanol extract of purple sweet potato leaves on LDL cholesterol level of hypercholesterolemia rat. The experiment was conducted experimentally using 2 groups of Wistar rats (each group consisting of 5 tails), namely the control group and the treatment group. Induction of hypercholesterolemia was performed with a high-fat diet for 7 days. The purple sweet potato ethanol extract was administered sonde in the treatment group with a quercetin dose of 2 mg /kg/day for 14 days and the measurement of LDL cholesterol was performed on days 10 and 24 by the CHOD-PAP method. Data were analyzed by Paired t-test. The results showed that LDL cholesterol level in the treatment group showed that 5 mice had decreased, the mean decrease of 32,49 mg/dl while control group 3 decreasing, mean 0,65 mg /dl. There was a significant difference (p <0.05) on changes in LDL cholesterol levels in the treatment group. Provision of purple sweet potato ethanol extract with quercetin dose of 2 mg/kg for 14 days can decrease LDL cholesterol level of hypercholesterolemia rat. From the results of this study is expected to optimize the utilization of purple sweet potato leaves (Ipomoea batatas (L.) Lam) as a drug for lowering LDL cholesterol.

scholarly journals PENGARUH EKSTRAK ETANOL DAUN UBI JALAR UNGU (Ipomoea batatas L.) TERHADAP LEUKOSIT DAN LIMFOSIT MENCIT JANTAN BALB/C YANG DI INDUKSI VAKSIN HEPATITIS B

2021 ◽  
Vol 4 (01) ◽  
pp. 59-71
Author(s):  
Siti Hamidatul Aliyah ◽  
Desi Sagita ◽  
Devi Kurnia
Keyword(s):  
Immune Response ◽  
Hepatitis B ◽  
Sweet Potato ◽  
Ipomoea Batatas ◽  
Leaf Extract ◽  
Control Group ◽  
Immunomodulatory Activity ◽  
Potato Leaf ◽  
Group I ◽  
Purple Sweet Potato

Abstract   Background: Purple sweet potato leaves (Ipomoea batatas L.) which has been known as immunomodulatory activity, which contains chemical compounds, such as vitamins, minerals, flavonoids (anthocyanin), especially iron found in a higher proportion in the leaves. Therefore, it is necessary to study the immunomodulatory activity of the leaf extract of purple sweet potato (Ipomoea batatas L.) on the immune response in Balb/c mice who induced by hepatitis B vaccines. Method: Inducing performed at day 0 and day 15. Mice with weights ± 20 g, which has been divided into normal control group, I, II, III, IV and V (each 5 mice), respectively were given orally by the leaf extract of purple sweet potato (Ipomoea batatas L.) (doses 100 mg / kg, 200 mg / kg, 300 mg / kg, 400 mg / kg and 500 mg / kg), normal controls (CMC Na 0,5%). With the volume of giving 1% of body weight in mice, performed during the study (20th days). Blood sampling is done via the tail vein (laeralis vein), to calculate the number of leukocytes (21st days) and lymph decision to count lymphocytes (21st days). Results: The results that the leaf extract of purple sweet potato (ipomoea batatas L.), especially a dose of 500 mg / kg as immunomodulatory activity can increase the number of lymphocytes proliferation cell. Conclusion: Purple sweet potato leaf extract has activity against the increase in the number of lymphocytes cell especially on dose 500 mg/kg BW who induced by hepatitis B vaccine that can stimulated an immune response.      

PENGARUH PEMBERIAN FRAKSI ETILASETAT KULIT UBI JALAR UNGU TERHADAP KADAR MALONDIALDEHID (MDA) SERUM MENCIT PUTIH JANTAN HIPERGLIKEMIA

2018 ◽  
Vol 8 (2) ◽  
pp. 144
Author(s):  
Ria Afrianti
Keyword(s):  
Blood Glucose ◽  
Statistical Analysis ◽  
Sweet Potato ◽  
Ethyl Acetate Fraction ◽  
Serum Levels ◽  
Negative Control ◽  
Control Group ◽  
Glucose Levels ◽  
Blood Glucose Levels ◽  
Purple Sweet Potato

This study aims to determine the effect giving of ethylacetate fraction of leather  purple sweet potato (Ipomoea batatas (L.) Lam, on levels of malondialdehyde (MDA) serum in mice hyperglicemia were induced with streptozocin dose of 50 mg/kgBW. Mice were divided into 5 groups, each group consisting of 3 tails, group I is a negative control, group II is a positive control, group III,IV and V is given ethylacetate fraction a dose of 100 mg/kgBW, 300 mg/kgBW, and 600 mg/kgBW. Ethyl Acetate Fraction leather purple sweet potato given orally for 15 days after the animal is declared hyperglicemia and measurement of blood glucose levels on 5, 10, and 15 day after giving test preparation in animal experiments. On the 16 day throughout the mice were taken serum levels measured malondialdehid. The statistical analysis results showed that giving of ethyl acetate fraction of leather purple sweet potato at a dose of 100 mg/kgBW, 300 mg/kgBW, and 600 mg/kgBW can lower blood glucose levels in mice hyperglycemia significantly (p<0.05). Malondialdehid levels on average in each group is 1.35 nmol/ml, 3.00 nmol/ml, 2.72 nmol/ml, 2.20 nmol/ml and 2.61 nmol/ml, the results of statistical analysis showed a decrease in melondialdehid serum levels were significantly (p<0.05), where a dose of 300 mg/kgBW is an effective dose for lowering blood glucose levels followed by decreased levels of malondialdehid which give effect approaching negative control.

scholarly journals Enhancement of the Antiobesity and Antioxidant Effect of Purple Sweet Potato Extracts and Enhancement of the Effects by Fermentation

Antioxidants ◽  
2021 ◽  
Vol 10 (6) ◽  
pp. 888
Author(s):  
Seul Gi Lee ◽  
Jongbeom Chae ◽  
Dong Se Kim ◽  
Jung-Bok Lee ◽  
Gi-Seok Kwon ◽  
...  
Keyword(s):  
Sweet Potato ◽  
Ipomoea Batatas ◽  
Radical Scavenging ◽  
The Body ◽  
Brown Adipocyte ◽  
Obese Mice ◽  
Adipose Tissues ◽  
White Adipocytes ◽  
Beige Adipocytes ◽  
Purple Sweet Potato

The browning of white adipocytes, which transforms energy-storing white adipocytes to heat-producing beige adipocytes, is considered a strategy against metabolic diseases. Several dietary compounds, such as anthocyanins, flavonoids, and phenolic acids, induce a brown adipocyte-like phenotype in white adipocytes. In this study, we demonstrated that purple sweet potato (Ipomoea batatas) extract (PSP) exhibited potent radical scavenging activity. In addition, PSP was found to contain large amounts of phenolic, flavonoid, and anthocyanin compounds; the amount of these compounds was affected by fermentation. Functionally, PSP-induced adipose browning in high-fat-diet (HFD)-induced obese mice. The administration of PSP significantly suppressed the body weight gain and abnormal expansion of white adipose tissues in the obese mice. The expression of adipose browning-related genes was higher in the inguinal white adipose tissues from the PSP-treated mice than those in the HFD-fed mice. Moreover, PSP-treated 3T3-L1 adipocytes formed multilocular lipid droplets, similar to those formed in the 3T3-L1 adipocytes treated with a browning induction cocktail. The PSP-treated cells had an increased expression level of mitochondria and lipolysis-related genes. The browning effects of PSP were enhanced by fermentation with Lactobacillus. This study, to our knowledge, is the first to identify a new mechanism to increase the antiobesity effects of PSP by inducing adipocyte browning of adipocytes.

Sign in / Sign up

Export Citation Format

Share Document