scholarly journals Біохімічні показники крові свиноматок за профілактики порушень обміну мінеральних речовин

2017 ◽  
Vol 19 (82) ◽  
pp. 71-76 ◽  
Author(s):  
N.G. Grushanska ◽  
V.М. Kostenko
Keyword(s):  
Blood Serum ◽  
Protein Content ◽  
Total Protein ◽  
Calcium Concentration ◽  
Emission Spectrometry ◽  
Control Group ◽  
Total Protein Content ◽  
Total Calcium ◽  
Pig Production ◽  
Experimental Group

Of all domestic animals, pigs suffer the most from deficiency of minerals. This is due to the specificity of pig production, feeding technology, retention and anatomical – physiological characteristics of pigs. In order to obtain the best qualities of pig production, it is necessary to make correction the ration for swines of various technological groups using mineral feed additives that take into consideration not only the physiological needs for minerals of the corresponding age groups and classes of swines but also the biogeochemical zone or the province where the farm is located. Also, conditions that accompany the emergence of a mineral deficit are taken into account. The development of new, eco-friendly, non-toxic means of preventing disorders of metabolism of minerals in pigs is a promising direction of veterinary medicine.The research was carried out ont the farm of the Kyiv region (northeastern biogeochemical zone). Biochemical parameters of blood were studied using biochemical analyzer «Labline-010» with standard sets of reagents. The content of chemical elements in water was investigated by the method of atomic emission spectrometry on the Optima 210 DV device.The paper presents the authors’ own research on determining the biochemical status of an organism of lactating sows in the northeastern biogeochemical zone of Ukraine at the prevention of microelementosis using new experimental eco-friendly means.The content of total protein, albumin, glucose, total calcium, inorganic phosphorus, cholesterol, bilirubin, activity of ALT, AST, AF in the blood serum of sows of the northern-eastern biogeochemical zone of Ukraine before and after the application of experimental drugs was investigated. The positive influence of prophylactic agents on the parameters of metabolism of proteins and calcium was defined.In the blood serum of the sows of the first experimental group, 28 days later, the total protein content on 5.3% lower and the total calcium concentration on 16.5% higher than the control group  of animals were found.In the blood serum of sows in the second experimental group,  28 days later, the total protein content on 8.3%  lower and the total calcium concentration on 19.9% higher than the control group of animals were found.

scholarly journals State of protein and mineral exchange in broiler-chickens for the use of Zinc and Mangane chelates

2019 ◽  
pp. 85-94
Author(s):  
V. Sakara ◽  
A. Melnyk ◽  
F. Markhenkov
Keyword(s):  
Total Protein ◽  
Broiler Chickens ◽  
Zinc Content ◽  
Calcium Content ◽  
Inorganic Phosphorus ◽  
The Body ◽  
Control Group ◽  
Total Protein Content ◽  
Total Calcium ◽  
Experimental Group

The results of the application of a complex of Zinc and Manganese chelates to protein, macro- and micro-mineral exchanges in the body of broiler chickens of the Cobb 500 cross-breed are presented. The administration of Zn and Mn chelates in a dose of 0,2 ml/l for chicken broilers of 23 days of age (1st experimental group) during 14 days influenced the increase in the total protein content to 35,3±0,8 g/l, which is 8 % higher compared with the control – 32,5±0,7 g/l (p<0,01 ) The concentration of total Calcium in serum of broiler chickens 37-day-old (end of trial) was higher in the first experimental group by 7,3 % (2,05±0,06 mmol/l) compared to control – 1,9±0,03 mmol/l. In the second group, where chelates were administered in a dose of 0,4 ml/l of water, the Calcium content was 2,0±0,02 mmol/l, which is 5 % more than the control group. The two-week application of Zn and Mn chelates also led to an increase in the level of inorganic Phosphorus in the serum of the first group to 2,6±0,12 mmol/l (+ 11,5 %) compared with the control group. After administration of chelates Zn and Mn at doses of 0,2 ml/l water, the Zinc content was higher by 4,3% (23,5±0,2 μmol/l) compared to control – 22,5±0,34 μmol/l (p<0,01). In the second group, where the dose of chelates was 0,4 ml/l, the concentration of Zinc was 23,6±0,16 μmol/l, which is more than 4,6 % for control (p<0,01). At the end of the study, the content of Manganese in serum of broiler chickens in the first group was 1,9±0,07 μmol/l (p<0,05), which is 10,4% higher compared with the control – 1,7±0,06 μmol/l (3rd sampling). The level of this element in the second group was 2,0±0,08 μmol/l (p<0,01), which is higher by 16,7 % relative to the control. At the end of the experiment, the coupon concentration in the first and second experimental groups was 12,6 and 9,3 % higher, compared with control, 7,0±0,37 μmol/l. In turn, the serum Ferrum content of the first group was greater by 9,3 % (19,3±0,69 μmol/l), and the second by 6,7 % (18,8±0,47 μmol/l) compared to the bird of the control group. Key words: chicken broilers, chelates, Zinc, Manganese, Cuprom, Ferrum, metabolism.

The effect of ethanol on some hematological parameters of the australian red claw crayfish (Cherax quadricarinatus)

2022 ◽  
pp. 85-91
Author(s):  
D. Skafar ◽  
D. Shumeyko
Keyword(s):  
Body Weight ◽  
Total Protein ◽  
Hematological Parameters ◽  
Group Versus ◽  
Control Group ◽  
Total Protein Content ◽  
Cherax Quadricarinatus ◽  
Total Hemocyte Count ◽  
Red Claw Crayfish ◽  
Experimental Group

Purpose: to study the effect of ethanol on the parameters of THC, the percentage of granulocytes and total protein in the hemolymph of the Red claw crayfish (Cherax quadricarinatus).Materials and methods. The object of this experiment was 26 males of the Australian red-clawed crayfish (Cherax quadricarinatus) weighing from 23 to 83 g. The individuals were evenly divided into two experimental groups - with an injection of ethanol and a control group without an injection of 13 crayfish for each group. The injection dose was 2515 mg per 100 g of body weight. A day after the introduction of ethanol, hemolymph was taken with a syringe from the ventral sinus, the syringe was pre-washed with a 4% EDTA-Na2 solution. Three parameters were determined: the total hemocyte count (THC), percent granulocytes and percent total protein content. Counting of hemocytes and determination of granulocytes were performed in a Goryaev chamber under a light microscope. The total protein was determined by the refractometric method.Results. Differences in THC and total protein between the groups were statistically unreliable (p>0,05). THC in the experimental group is 36% more than in the control group. The total protein after the introduction of ethanol actually increased by 0,7%, and relatively by 14%. There were statistically different indicators of the proportion of granulocytes (p<0,05) - the average value of 33,1% in the experimental group versus 24,5% in the control group. A reliable (p<0,05) strong feedback was revealed between the total protein and the mass of individuals in both experimental groups, while in the experimental group there is a visible shift in the values of dependent hemolymph indicators towards an increase in smaller individuals.Conclusion. A single injection of ethyl alcohol with a dosage of 2515 mg per 100 g of body weight into the hemolymph of C. quadricarinatus does not cause significant changes in the THC and total protein after 24 hours. At the same time, the proportion of granulocytes actually increases by 9%, relative to 37%. This may indicate that granulocytes are involved in the formation of cancer defense mechanisms when exposed to toxic substances. The effect of different dosages of ethanol injections and the duration of its effect on hematological parameters requires additional consideration. It is necessary to investigate its effect on other indicators, such as the pH and buffer capacity of the hemolymph, the concentration of hemocyanin, glucose, lactates and calcium.

scholarly journals Effect of fundectomy, antrectomy and gastrectomy on pancreatic and brush border enzyme activity in rats

2018 ◽  
Vol 74 (1) ◽  
pp. 6031-2018
Author(s):  
MAŁGORZATA KAPICA ◽  
IWONA PUZIO
Keyword(s):  
Enzyme Activity ◽  
Protein Content ◽  
Brush Border ◽  
Total Protein ◽  
Control Group ◽  
Total Protein Content ◽  
Surgical Removal ◽  
Hormonal Changes ◽  
Serum Samples ◽  
Distal Jejunum

The aim of our study was to investigate the possible effects of the removal of different parts of the stomach (fundectomy, antrectomy, gastrectomy) on the total protein content and enzyme activity in the pancreas and the brush border of the intestinal mucosa. Twenty-four 2.5-month-old male Wistar rats were divided into four groups: sham-operated animals (SHO) and those subjected to gastrectomy (Gx), fundectomy (Fx), and antrectomy (ANT). After a six-week experiment, the rats were sacrificed, and blood was collected for further gastrin analysis in serum. Samples of the pancreas, duodenum, and jejunum (proximal part in 25% of length, middle part in 50% of length, and distal part in 75% of length) were collected to determine the total protein content and enzyme activity. The rats subjected to fundectomy, antrectomy and gastrectomy showed an increased total protein content and enzyme activity (amylase, trypsin) in pancreatic tissue. They exhibited an increase in the total protein content in the homogenates of the mucosa of the proximal, middle and distal jejunum, compared to the control, and a statistical increase in maltase activity. Compared with the control group, the rats subjected to Fx and ANT showed a decreased sucrase activity in the homogenates of the mucosa of the duodenum and of the proximal, middle and distal jejunum. In the gastrectomized rats, there was a statistically significant increase in the total protein content in the homogenates of the mucosa of the jejunum, compared to the control, while the activities of lactase and sucrase were decreased. There was a statistically significant increase in the gastrin level in all experimental groups (Fx, ANT, Gx). We suggest that surgical removal of a part of the stomach radically changes the level of hormones that determine many functions of the organism. Hormonal changes may have an impact on the pancreas and the activity of brush border enzymes. .

scholarly journals The biochemical indicators of a cow organizm at the prevention of microelementosis

2018 ◽  
Vol 20 (83) ◽  
pp. 183-188
Author(s):  
N.G. Grushanska
Keyword(s):  
Total Protein ◽  
Chemical Elements ◽  
Organic Phosphorus ◽  
Zinc Content ◽  
Emission Spectrometry ◽  
Control Group ◽  
Total Calcium ◽  
Creatinine Concentration ◽  
Animal Group ◽  
Mineral Substances

Correction of cattle feed, taking into account the physiological needs of their organism in the mineral substances of the corresponding biogeochemical zone or the province, where the farm is located, as well as the conditions that accompany the emergence of the deficit, promotes the obtaining of quality livestock products. An urgent task of the present is the search for eco-friendly, non-toxic and highly effective, preventive drugs of complex action, which positively affect the metabolism of mineral substances in the animal organism. The research was carried out on the farm of Kyiv region (central biogeochemical zone). We studied the biochemical parameters of blood and saliva using biochemical analyzer «Labline-010» with standard sets of reagents. The content of chemical elements in blood and saliva was investigated by the method of atomic emission spectrometry with inductively coupled plasma using Optima 210 DV device. The paper presents the research on determining the biochemical status of an organism of lactating cows at the prevention of microelementosis using new experimental eco-friendly drug. The content of total protein, albumin, glucose, total calcium, non-organic phosphorus, cholesterol, total bilirubin, aluminium, chrome, copper, zinc, nickel and activity of ALT, AST, ALP, GGT in cow blood and saliva in the first and 28th day for the use of the drug oxyminkor. The diagnostic informativity of saliva content according to the content of mineral substances is determined. In cow blood at the use of the drug oxyminkor for 28th day of the experiment, the content of total protein was determined to be 17% higher, albumin was 19% higher, total calcium was 36 % higher and creatinine concentration was 25% lover, compared with the indicators of the control group. In the cow saliva at the use of the drug oxyminkor on the 28th day of the experiment, the content of zinc was 2.1 times higher, compared with the control animal group. The correlation coefficient for zinc content in cow blood and saliva was 0.80, which points the informativity of this indicator. We defined the positive influence of the prophylactic agent on the metabolism of proteins, minerals and functional state of the cow liver. The introduction of new methods of non-invasive diagnosis and the development of ecological, non-toxic agents for the prevention of mineral disbolism among cows is a promising area of research.

scholarly journals Dynamics of total protein content in blood of sows depending on peculiarities of nervous system activity

2019 ◽  
Vol 21 (94) ◽  
pp. 51-56
Author(s):  
R. Postoi ◽  
V. Karpovskyi ◽  
O. Danchuk ◽  
D. Kryvoruchko
Keyword(s):  
Nervous System ◽  
Autonomic Nervous System ◽  
Blood Serum ◽  
Protein Content ◽  
Total Protein ◽  
Weak Type ◽  
Nervous Activity ◽  
Total Protein Content ◽  
Experimental Animals ◽  
Higher Nervous Activity

The article presents the results of studies on the dynamics of total protein content in blood serum of sows depending on the cortical and vegetative mechanisms of regulation under exposure to technological stimulus. Experiments were carried out on 3 years old pigs of large white breed. Types of higher nervous activity in pigs were determined using method of conditioned food reflexes. According to the studying of conditioned reflex activity 4 experimental groups were formed, 5 animals in each. The 1-st group consisted of sows with strong balanced mobile, the 2-nd – strong balanced inert, the 3-rd – strong unbalanced, and the 4-th – weak types of higher nervous activity. Then in experimental animals we studied the tone of the autonomic nervous system using trygeminovagal test, by the results of which we formed 3 experimental groups (normotonics, sympathicotonics, vagotonics), 5 animals in each. Before exposure to technological stimulus and in 1, 3, 7, 14 and 28 days after its impact, blood samples were taken for biochemical studies in all experimental animals. Before exposure to technological stimulus content of total protein in swine blood serum between animals with strong types of higher nervous activity did not differ significantly. Animals with weak type of higher nervous activity had lower level of this metabolite than animals with strong balanced mobile type. After exposure to technological stimulus the total protein level in swine blood serum significantly decreased. In sows with strong balanced mobile type, the content of total protein starting from the 7-th day after exposure to technological stimulus returned to values that were before stress factor impact. At the same time, in sows with weak type of higher nervous activity, the level of total protein in blood returned to the reference values only in 28 days after exposure to technological stimulus. The basal level of total protein in blood serum in pigs with different tone of autonomic nervous system didn’t differ significantly. In sows normotonics was found a significantly higher content of total protein in blood serum than in sows vagotonics and sympathicotonics during first week after exposure to stress factor.

scholarly journals The effect of herbal phytoextract on metabolic parameters of sows and piglets

2021 ◽  
Vol 22 (3) ◽  
pp. 428-435
Author(s):  
A. A. Ivanovsky ◽  
N. A. Latushkina ◽  
E. Yu. Timkina
Keyword(s):  
Total Protein ◽  
Biochemical Parameters ◽  
Control Group ◽  
Total Protein Content ◽  
Herbal Extracts ◽  
Control Groups ◽  
Normal Range ◽  
Experimental Group ◽  
Experimental Pigs

The purpose of the research was to study the effect of a phytocomplex containing herbal extracts of R. carthamoides, S. coronata, F. ulmaria on blood biochemistry of milking sows, the development and viability of piglets born by the time of weaning. For the experiment, the animals were divided into an experimental (Phytoadditive) and a control (SPK-2 compound feed) groups, 10 animals each. Phytoadditive in dry form was introduced into the diet of pigs of the experimental group during the sucking period (30 days) daily, once per head per day - 10 grams (individually). Before the beginning and at the end of the experiment, the blood of sows was examined for biochemical parameters. The multiplicity, the quality of the piglets born, the weight of the nest and one pig after birth and before weaning, as well as viability were determined. As the result, it was found that the concentration of the investigated extractives in the Phytoadditive was 13.5 g/kg, of which 10.5 g were ecdysteroids and 3.0 g of flavonoid rutin. Significant changes in the total protein content in the blood compared with the beginning of the experiment were noted in experimental pigs from 60.2±0.5 to 67.3±0.2 g/l (P <0.05) and in the control group from 61.0±0.2 to 66.1±0.1 g/l (P <0.05), the "Ca" content from 2.1±0.01 to 2.7±0.02 mmol/l (P <0.05) in the experimental group and from 2.0±0.01 to 2.5±0.02 mmol/l (P <0.05) in the control groups, while being within the normal range. The amount of albumin significantly increased only in the experimental group from 40.8±1.2 to 49.3±2.8 g/l (P <0.05), in the control from 42.2±2.1 to 46.0±1.5 g/l (P> 0.05). The number of piglets with low viability in the experimental group was 2 times less than in the control group. The viability of piglets in the experimental group by the time of weaning was 92.6 %, in the control ‒ 83.2 %.

scholarly journals Use of sweet yellow clover (Melilotus officinalis) extract in sheep feeding

2021 ◽  
Vol 291 ◽  
pp. 02007
Author(s):  
Elena Ulrikh ◽  
Olga Babich ◽  
Stanislav Sukhikh
Keyword(s):  
Blood Serum ◽  
Total Protein ◽  
Live Weight ◽  
Control Group ◽  
Melilotus Officinalis ◽  
Experimental Group ◽  
Different Doses

During the study, it was found that an increase in the dose of sweet yellow clover extract from 0.5 to 2.5 mg/kg of live weight per day contributed to an increase in the number of: hemoglobin by 1.83 g/l (1.79%), leukocytes by 0.14 thousand 109/l (2.34%), total protein in blood serum of sheep by 0.43 g/l (0.7%), glucose in blood of sheep by 0.18 mmol/l (4.19%), calcium in blood of sheep by 0.14 mmol/l (5.32%), phosphorus in blood of sheep by only 0.08 mg/100 ml (1.6%), contributed to an increase in the live weight of sheep by 220 g (2.18%). In the control group, two sheep out of six got bronchitis. The percentage of sheep with bronchitis was 33.33%. After feed to the sheep different doses of sweet yellow clover extract, the sheep in groups I and II of the experimental groups had one sheep each (16.67%), while in groups III, IV and V there were no sheep with bronchitis. Thus, increasing the sweet yellow clover dosage in sheep’ diet reinforced their immunity. In the control group and in the first experimental group, one sheep out of six came down with dyspepsia. The percentage of sheep with dyspepsia was 16.67%. After feed to the sheep doses of sweet yellow clover extract, there were no dyspeptic diseases in sheep.

scholarly journals Stress state and immunological status of young pigs

2019 ◽  
pp. 95-98
Author(s):  
M. V. Chornyi
Keyword(s):  
Stress State ◽  
Blood Serum ◽  
Bactericidal Activity ◽  
Total Protein ◽  
Live Weight ◽  
Natural Resistance ◽  
Control Group ◽  
Immunological Status ◽  
Young Pigs ◽  
Experimental Group

The paper presents the results of studies on the effect of regrouping of piglets at different ages on the resistance of their organisms, immunological status, productive qualities and safety of young pigs. The work was performed at “Stas” LLC on large white breed pigs and their crossbreeds. For the experiment, three groups of piglets were formed from suckling sows — analogues. In the experiment 60 animals from birth up to two months of age were used, 20 animals in each experimental group. The control group of piglets was raised from birth up to 60 days of age in nests, and then they were moved to the rearing workshop; Experimental 1 group was kept up to 10 days old in nests, and then a one-time rearrangement was performed according to live weight. Piglets from experimental group 2 were regrouped according to live weight at 5, 10, 15, 21, 30 days of age. To assess the natural resistance of piglets due to the above groups, hematological, biochemical (total protein, protein fractions) methods, immunological (immunoglobulins of classes IgA, IgM, IgG), and natural resistance (bactericidal activity of blood serum) were used, serum lysozyme activity (LASK), phagocytic neutrophil activity (FAN), phagocytic index (FI), ethological, zootechnical, mathematical. To assess the natural resistance of piglets the following research methods were used: generally accepted zoo veterinary methods (live weight, morbidity, safety), hygienic (microclimate conditions, sanitary regime), hematological (morphological blood composition), biochemical (total protein, protein fractions), immunological (immunoglobulins of classes IgA, IgM, IgG), natural resistance (bactericidal activity of blood serum), ethological and mathematical methods. Results of work. It was found that when growing piglets, nesting, without moving, caused their growth and development without any noticeable physiological disturbances, both from the blood and ethology. In animals (Experimental-1 groups) which underwent a single regrouping in a 10 day reward, their lag in live weight by 12.65% was recorded, compared with the control, with two and three-time movement (Experimental-2) — by 16.10%. Their SSPs were 28% less and 14.7% lower than in the control piglets. Patients with symptoms of diarrhea were identified in the control: in 3–4 daily reimbursement — 1%, in Experimental-1 — 5%, Experimental-2 — 10–15%. The resistance of young animals to gastrointestinal diseases according to the Melenberg coefficient in the Experimental-2 group was 6.13–7.48, in control group it was 0.34, and the safety did not exceed 80.2%. According to the level of immune status, animals from Experimental-2 group were inferior: by BASK — by 25.15% (28 days of age), by LASK — by 7.56%, by FAN — by 10.7% compared with peers from Experimental-1 groups. By the number of eosinophils (Experimental-2 group), characterizing the stress state of piglets, their decrease was observed within 10–12 days, not more than 5 days — in animals from Experimental-1 group and up to 3 days — from control

scholarly journals Functional condition of broiler chicken liver using multienzyme preparation «Natyzyme»

2020 ◽  
pp. 64-69
Author(s):  
O. V. Matsenko ◽  
◽  
L. L. Kusch ◽  
Y. V. Maslak ◽  
◽  
...  
Keyword(s):  
Blood Serum ◽  
Protein Content ◽  
Aspartate Aminotransferase ◽  
Broiler Chickens ◽  
Efficiency Index ◽  
Alanine Transaminase ◽  
Chicken Liver ◽  
Synthetic Activity ◽  
Control Group ◽  
Experimental Group

The results of investigation using multienzyme preparation «Natyzyme» for broiler chickens to provide ultimate digestion of feed by preventive treatment of digestive system organs, increase of poultry growth intensity and correction of physiological spectrum of homeostasis have been shown in this article. Fifty chickens of cross Ross 308 of first (experimental) group since the sixth day of growth were inserted preparation «Natyzyme» added to feed in a dose of 35 gr on 100 kg of feed during 30 days, while the poultry of second (control) group was not used that preparation. The influence of enzyme preparation «Natyzyme» was estimated on 14th and 36th day of poultry birth according to analysis of productive indices, the results of biochemical and histological researches. It was defined the chick livability and liveweight gain of poultry during the period of growth, feed conversion ratio and EEI (European efficiency index) of poultry operation. Blood sampling was carried out in above mentioned period to define indices, characterized functional liver state: protein contain, albumin, alanine transaminase, aspartate aminotransferase, cholinesterase, that were investigated spectrophotometrically. The liver samples were taken for histological research on the 36th day of poultry birth. Obtained digital data were processed mathematically of variation statics. The use of preparation «Natyzyme» contributed to broiler body weight gain on 5.1 % (р ≤ 0,05), viability – on 4.0 %. The results of biochemical researches of blood serum showed higher protein content in chickens of experimental group to 14 and 36th day of experience on 3.2 and 8.9 % (р ≤ 0,05) accordingly. In connection with increasing protein content the index of synthetic activity of liver has grown – cholinesterase contain: on 14th day of birth on 7.1 %, 36th day of birth – on 14.6 % (р ≤ 0,05). The indices of enzymes transamination activity - alanine transaminase, aspartate aminotransferase in blood serum of experimental chicken group on 14th and 36th day of birth accurately have not differed from control data. According to the histological analysis of liver the positive influence of multienzyme preparation on the chicken broilers body has been diagnosed. Unlike the chicken liver of control group, where the presence of liver beam decomplexation in the composition of particles and fatty degeneration of hepatocytes was detected, the chickens of experimental group did not have the such features of the microscopic structure. As a result, the use of preparation «Natyzyme» contributed to greater growth and safety of broiler chickens, reducing of feed costs per 1kg of poultry gain, increasing the European efficiency index of poultry, had a positive effect on the functional state of the liver, that blood biochemical results and data of histological researches have been proved, that shows the advantages of its use in the feeding of broiler chickens.

scholarly journals Етіологія та клініко-біохімічні показники крові за аліментарної остеодистрофії корів

2017 ◽  
Vol 19 (73) ◽  
pp. 79-83
Author(s):  
L. Slivinska ◽  
S. Demydjuk ◽  
А. Shcherbatyy ◽  
V. Fedorovich ◽  
I. Tyndyk
Keyword(s):  
Clinical Study ◽  
Body Temperature ◽  
Total Protein ◽  
Control Group ◽  
Total Protein Content ◽  
Total Calcium ◽  
Carotene Content ◽  
Excess Calcium ◽  
Caudal Vertebrae ◽  
Mucous Membranes

Established etiological factors of nutritional osteodystrophy cows LLC Danylo Galician Yavoriv district, Lviv region. A clinical study on nutritional osteodystrophy cows. A decrease in productivity, alotriophagy, strained course, lameness, thickening and tenderness of the joints, softening of the caudal vertebrae, in severe cases – the formation of animals have been established. The fatness of most animals was below average, hypotension and atony of the predniegens were observed, body temperature was within the norm. In 50% of cows pallor of the mucous membranes was noted, in 10 cows – tachypnea (47.0 ± 0.45) and tachycardia (110.0 ± 3.65). In 10% of the cows, the thorax was pulled up, and the vertebral trunk – curved upwards. In 5 cows, fluctuations of the teeth were noted. Analyzing the diet of feeding cows, a shortage of phosphorus (10–15 g), cobalt (4–5 mg), zinc (27–110 mg), iodine (5–6 mg), sugar (120–180 g), copper (24–26 mg), carotene content (20–30 mg), excess calcium (14–28 g), iron (1400–2200 mg), manganese (210–360 mg). In the blood of experimental cows, the level of hemoglobin was reduced (by 10.6%), the number of erythrocytes (18.8%) in comparison with the animals in the control group. Leukoformula in cows was characterized by a decrease in the number of basophils (9%), eosinophils (47.6%), stabnoid (82.3%) and segmented (4%) neutrophils, monocytes (13.3%) and an increase in lymphocyte count (11% ). In the blood serum of the experienced, a decrease in the total protein content (7.2%), total calcium (26.3%), carotene (22.8%) and cobalt (58.8%) was established.

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