Analysis of Wound Healing from Andaliman Fruit Essential Oil Ointment (Zanthoxylum Canthopodium Dc.) on Wistar Rats (Rattus Norvegicus)

Injuries are cases of injury that are often experienced by every human being. A wound is the loss or damage of some body tissue. Many andaliman fruits contain many phytochemicals such as phenols, saponins, flavonoids, triterpioids, and alkaloids. Its terpenoid content has antioxidant and antimicrobial activity repellents. Iris / incision wounds, which are wounds caused by sharp object slices such as knives, cause damage to vessels that are large enough if the slice is deep enough. This study aims to find out the effect of Andaliman Fruit Essential Oil Saleb (Zanthoxylum acanthopodium DC.) on wound healing in white rats. This experimental study with the approach of Pre-test and Post-test group only control design was conducted January to February 2021, at the Herbarium Medanese FMIPA USU, the Pharmacognosive Laboratory of the Faculty of Pharmacy USU, and the Laboratory of Pharmaceutical Pharmacology USU. The samples were Andaliman Fruit (Zanthoxylum acanthopodium DC.), and male white rats. The number of samples using frederer formula, so determined the number of as many as 25 rats selected randomly divided into 5 groups. Data analysis uses the normality test statistical data test, and the ANOVA test. The results of the study that andaliman fruit extract has several bioactive compounds such as alkaloids, flavonoids, saponins, and tannins that play a role in wound healing. The optimum concentration of Saleb Andaliman fruit essential oil that can heal wounds in white rats is 5%. The highest percentage of cures on the 14th day was in positive control (Bioplacenton®) which was 95% and followed by an extract of 5% v/v with a cure percentage of 90%. Essential oil of andaliman fruit extract has the ability to approach Bioplacenton® in healing wounds in mice.

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Esktrak ikan haruan (Channa striata) menurunkan jumlah makrofag pada fase inflamasi proses penyembuhan luka (Extract of haruan (Channa striata) decreases macrophages count in inflammation phase of wound healing process )

Journal of Dentomaxillofacial Science ◽

10.15562/jdmfs.v14i1.417 ◽

2015 ◽

Vol 14 (1) ◽

pp. 6

Author(s):

Putri Sri Hartini ◽

Nurdiana Dewi ◽

Lisda Hayatie

Keyword(s):

Wound Healing ◽

Treatment Group ◽

Wistar Rats ◽

Healing Process ◽

Positive Control ◽

Wound Healing Process ◽

Treatment Groups ◽

Accelerate Wound Healing ◽

Post Test ◽

Channa Striata

Haruan is a kind of freshwater fishes commonly found in South Kalimantan. Haruan extract contains nutritional substancessuch as albumin, Zn, Fe, Cu, and unsaturated fatty acid which can help accelerate wound healing. The aim of this studywas to histopathologically assess the effect of 100%, 50%, and 25%haruan extract compared to aquadest and ibuprofenon macrophages count in inflammation phase of wistar rats’ buccal mucosa wound healing. This research was a trueexperimental with post test-only with control design. Samples used were 30 wistar rats divided into 5 groups, 100%, 50%,25% haruan extract treatment groups, ibuprofen treatment group as positive control, and aquadest treatment group asnegative control. Mean macrophages counted on day 3 of 100%, 50%, 25% haruan extract treatment groups aquadest,and ibuprofenl were 2.05, 4.4, 3.9, 3.3, 2.4 respectively. In conclusion, haruan extract had a significant effect in decreasingmacrophages count in inflammation phase of wound healing.

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The Effectiveness of the Antiinfamation Combination Gel of Okra Fruit (Abelmoschus esculentus) Extracts and Shallots Extract (Allium cepa L.)

Journal Of Tropical Pharmacy And Chemistry ◽

10.25026/jtpc.v5i1.228 ◽

2020 ◽

Vol 5 (1) ◽

Author(s):

Sara Nurmala ◽

Moerfia Moerfia ◽

Santi Novianti

Keyword(s):

Wound Healing ◽

Allium Cepa ◽

The United States ◽

Abelmoschus Esculentus ◽

Male Rats ◽

Cell Multiplication ◽

Fruit Extract ◽

White Rats ◽

Allium Cepa L ◽

Anti Inflammatory

Wound is a condition that is the destruction of the unity or network components, which are specifically found the substance of tissue damaged or lost, either damage the continuity of the skin, mucous membranes and bones or other organs. Wound healing is a very complex process involving many cells. The process of wound healing is portrayed as occurs in biological surgical wound occurred. Several phases of wound healing are: the process of inflammation (inflammatory), the process of cell multiplication (proliferation) and cell maturation process (maturase). Okra is a plant that allegedly came from Southeast Asia. Okra has grown in many countries in almost all over the world. In the UK okra known as the Lady's Finger, in India it is called by the name of Bhindi while the United States called Gumbo. In Indonesia, this plant also has a local name that is Rabamea (Bima), Coffee Java (Java), Arabian Coffee (Sulawesi), Hoinu (Southeast Sulawesi), but better known by the name of okra. This study aims to determine the effective concentration and determine the length of time of wound healing using a combination gel okra fruit extract (Abelmoschus esculentus) And shallots (Allium cepa L.) as an anti-inflammatory against cuts in male white rats (Sprague -Dawley) with a length of observation for 9 days. This study uses Betadine® ointment as a positive control. The results showed the combination of fruit extract gel okra 6% and shallots 2% contained in the formula 3 most effective as an anti-inflammatory against cuts in male rats by administration for 6 days.

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Effects of Ciplukan Fruit Extract (Physallis peruvenia) on T4 levels in White Rats

Biomedical Journal of Indonesia: Jurnal Biomedik Fakultas Kedokteran Universitas Sriwijaya ◽

10.32539/bji.v5i3.9872 ◽

2019 ◽

Vol 5 (3) ◽

pp. 121-125

Author(s):

Nita Parisa

Keyword(s):

Thyroid Hormone ◽

Positive Control ◽

Negative Control ◽

Research Subjects ◽

Fruit Extract ◽

Hormone Synthesis ◽

Fruit Extracts ◽

Physalis Peruviana ◽

White Rats

Hyperthyroidism is a condition where there is an increase in thyroid hormone synthesis and secretion so that the level of thyroid hormone in the blood increases. In 2013, data showed 0.4% of Indonesia's population experienced hyperthyroidism. Ciplukan fruit (Physalis peruviana) is an Indonesian herb that is thought to have an effect on thyroid hormone. This research is an in vivo experimental study. The research subjects were 30 Wistar strain white rats divided into 5 groups: negative control (distilled water), positive control (PTU 100 mg / kgBW), groups 3, 4, and 5 Ciplukan fruit extracts at a dose of 250 mg/kgBW, 500 mg/kgBW, and 1000 mg/kgBW. The results of the examination showed a decrease in T4 levels in the treatment with positive control and administration of ciplukan fruit extracts. There was a difference in the reduction in T4 levels for the group giving ciplukan extract doses of 1, 2, and 3 (p <0.05). It can be concluded that Ciplukan fruit extract with a dose of 250 mg/kgBW, 500 mg/kgBW, and 1000 mg/kgBW has potential as an anti-hyperthyroidism.

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Efek Pemberian Ekstrak Daun Pegagan (Centella Asiatica) Terhadap Penyembuhan Luka Sayat Pada Tikus Putih Jantan (Rattus Norvegicus) Galur Wistar

Jurnal Ilmiah Kedokteran Wijaya Kusuma ◽

10.30742/jikw.v9i1.664 ◽

2020 ◽

Vol 9 (1) ◽

pp. 21

Author(s):

I Made Subhawa Harsa

Keyword(s):

Wound Healing ◽

Rattus Norvegicus ◽

Leaf Extract ◽

Centella Asiatica ◽

Negative Control Group ◽

Positive Control ◽

Negative Control ◽

Control Group ◽

White Rats ◽

Wistar Strain

Wound refers to partly damage or loss of body tissue. One way that has been used by the people in helping the process of wound healing is the use of medicinal plants, such as gotu kola (Centella asiatica). Gotu kola (Centella asiatica) is kind of plant that found almost in all territories of Indonesia which functions as a cell revitalizing substance which quickens wound healing those are burnt, skin ulcer and to prevent keloid. This study is aimed to know the effect of giving gotu kola leaf extract (Centella Asiatica) against to the healing of the wound incision on male white rats (Rattus Norvegicus) wistar strain. This study is a pure experimental research using the randomized post test only control group design with the subjects are 30 male white rats (Rattus norvegicus) wistar strain that were divided into 3 groups, namely K1, K2, and K3. The incision wound were made on the back of all white rat in each group with a length of ± 2 cm and a depth of ± 2 mm. The wound in the negative control group (K1) was not given any substance; in the positive control group (K2) was given povidone iodine 10%; and in the treatment group (K3) was given gotu kola leaf extract at a dose of 37,5 mg/150 grBB. The study was done for 10 days. Furthermore, wound observation and monitoring is held. Then the results were processed by using One Way ANOVA. The result study of wound incision healing on male white rats (Rattus norvegicus) wistar strain shows that there is significant difference among the negative control group (K1), the positive control group (K2), and the treatment group (K3) on One Way ANOVA test which significant p-value are 0,027 (p < 0.05). The result of Post-Hoc LSD test also shows a significant differences among the K1 and K3 groups, and K2 and K3 groups which significant p-values are 0,011 and 0,038 (p < 0,05). From these results, it can be concluded that gotu kola leaf extract (Centella asiatica) effective on quickens wound healing of the wound incision on male white rats (Rattus norvegicus) wistar strain.

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Test of the Potential of Ethanol Extracts, Simplician Medium and Forest Umbi Juice (Eleutherine bulbosa (Factory) Urb.) against Blood Glucose Levels of Rats, and Histopatology Ratkreas Rat (Rattus norvegicus) Hypercolesterolemia Model Diabetic

Journal Of Tropical Pharmacy And Chemistry ◽

10.25026/jtpc.v5i1.229 ◽

2020 ◽

Vol 5 (1) ◽

Author(s):

Joni Tandi ◽

Tien Wahyu Handayani ◽

Ni Wayan Ika Purwasih

Keyword(s):

Blood Glucose ◽

Test Group ◽

Ethanol Extract ◽

Positive Control ◽

Pancreatic Tissue ◽

Glucose Levels ◽

Onion Bulbs ◽

White Rats ◽

Blood Glucose Levels ◽

Group I

This study aims to examine the presence or absence of secondary metabolite compounds in ethanol extract, simplicia steeping and forest onion tuber juice, in male glucose parameters and pancreatic histopathology. Using test animals as many as 30 male white rats divided into 6 groups and each group consisted of 5 rats with group I details as normal controls, group II as negative controls given 0.5% Na-CMC suspension, group III as positive control who were given Metformin 45 mg / kg BB and IV, V, VI as the test group were given ethanol extract, simplicia extract and forest onion tuber juice with each dose of 20 g / kg BW. The results showed that there were secondary metabolites in the ethanol extract of forest onion tubers, namely flavonoids, saponins, alkaloids, steroids and tannins, giving ethanol extract of forest onion bulbs had the effect of reducing blood glucose levels in white mice with a dose of 20g / kgBW with an average of 132 and ethanol extract of forest onion bulbs at a dose of 20g / kg BB with an average of 0.5 can regenerate pancreatic tissue.

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Formulation and Evaluation of Helichrysum italicum Essential Oil-Based Topical Formulations for Wound Healing in Diabetic Rats

Pharmaceuticals ◽

10.3390/ph14080813 ◽

2021 ◽

Vol 14 (8) ◽

pp. 813

Author(s):

Marijana Andjić ◽

Biljana Božin ◽

Nevena Draginić ◽

Aleksandar Kočović ◽

Jovana N. Jeremić ◽

...

Keyword(s):

Wound Healing ◽

Essential Oil ◽

Redox Status ◽

Positive Control ◽

Negative Control ◽

Diabetic Rats ◽

Wound Management ◽

Hydroxyproline Content ◽

Wound Model ◽

Repairing Effect

As proper wound management is crucial to reducing morbidity and improving quality of life, this study evaluated for the first time the wound healing potential of H. italicum essential oil (HIEO) prepared in the form of ointment and gel in streptozotocin-induced diabetic wound models in rats. After creating full-thickness cutaneous wounds, forty-eight diabetic rats were divided into six groups: (1) negative control; (2) positive control; (3) ointment base; (4) gel base; (5) 0.5% HIEO ointment (6) 0.5% HIEO gel. Wound healing potential was determined by the percentage of wound contraction, hydroxyproline content, redox status, and histological observation. A significant decrease in the wound size was observed in animals treated with HIEO formulations compared with other groups. The HIEO groups also showed a higher level of total hydroxyproline content, and more pronounced restitution of adnexal structures with only the underlying muscle defect indicating the incision site. Hence, our results legitimate the traditional data of the pro-healing effect of HIEO because HIEO in both formulations such as gel and ointment exhibited the significant wound repairing effect in the incision wound model.

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Pengaruh Spirulina terhadap VEGF dari Trofoblast Tikus Putih Hamil Strain Wistar yang Diinduksi Interleukin 6

Jurnal Ilmiah Kedokteran Wijaya Kusuma ◽

10.30742/jikw.v9i2.750 ◽

2020 ◽

Vol 9 (2) ◽

pp. 123

Author(s):

Harry K Gondo ◽

Elizabeth Haryanti

Keyword(s):

Interleukin 6 ◽

Positive Control ◽

Control Group ◽

Vascular Endothelial ◽

Control Group Design ◽

Blue Green Algae ◽

White Rats ◽

Post Test ◽

Endothelial Growth Factor ◽

Vegf Level

Preeclampsia is a pregnancy disorder with hypertension as one of its common symptoms. Preeclampsia in pregnancy is characterized by increasing of proteinuria, blood pressure and also interleukin 6. Spirulina sp is a thread like shaped blue-green algae, similar to chain of cylindrical cells with 1 to 12 μm diameter of cell membrane. The purpose of this research was to know the effect of spirulina on VEGF level in improving of trophoblast function in pregnant white Rats Wistar. The design of the study was laboratory experimental with post-test only control group design. Twenty-five rats with preeclampsia model induced by interleukin 6, were divided into 5 groups. The groups were control group, positive control group (P0) injected by interleukin-6 dose of 5 ng/ 100 gram body weight (BW), group with spirulina dose of 10 mg/100 gram BW (P1), group with spirulina dose of 20 mg/100 gram BW (P2); and group with spirulina dosage 40 mg/100 gram BW (P3). The Vascular endothelial growth factor (VEGF) levels were in experimental groups measured compared to control. The averages of group spirulina were 81.31 (10 mg/100 gram BW), 65.99 (20 mg/100 gram BW), and 49.62 (40 mg/100 gram BW). In comparison with control group (62.70), VEGF level in group administered by spirulina dose of 20 mg/100 gram BW were close to control group.

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Formula of Yellow Kepok Banana (Musa acuminata x Musa balbisiana) Corm Extracts As Antiinflamation

Buletin Penelitian Tanaman Rempah dan Obat ◽

10.21082/bullittro.v30n2.2019.100-110 ◽

2020 ◽

Vol 30 (2) ◽

pp. 100

Author(s):

Desy Muliana Wenas ◽

Lisana Sidqi Aliya ◽

Wisma Merry Anjani

Keyword(s):

Wound Healing ◽

Positive Control ◽

Negative Control ◽

Inflammatory Activity ◽

Sprague Dawley ◽

Treatment Groups ◽

Accelerate Wound Healing ◽

White Rats ◽

Anti Inflammatory ◽

Post Hoc

Bananas have many types and benefits. One of them is the Yellow Kepok Banana. Banana stem, leaf midrib, and corm contain phytochemicals compound as medicinal properties. The study aimed to determine the effect of the yellow kepok banana corm extract formula as an anti-inflammatory. Banana corm was cut into 0.3 x 1 x 3 cm pieces, dried in the oven with temperature 60 °C for 6 hours, then made 40 mesh powder. The powder extracted using 70 % ethanol by maceration technique for 24 hours, repeated three times. Anti-inflammatory testing was carried out on male Sprague Dawley white rats. A total of 24 white rats were divided into six treatment groups, consists of different concentrations of the extract (10, 15, and 20 %), positive control (commercial ointment), negative control, and a normal control (giving a wound without being given any formula). The experiment was designed as a randomized complete, with four replications, data were analyzed using ANOVA and the Post Hoc Games Howell Test. The results showed that the yellow kepok banana extract formula had the potential for wound healing. The 20 % extract formula can accelerate wound healing in a day than the 10 and 15 % formulas. The speed is shown by wound healing without scars from the 20 % extract formula, which occurs earlier than other formulas. The anti-inflammatory activity of the 20 % formula was comparable to the positive control of commercial ointment. The anti-inflammatory activity of the yellow kepok banana ointment formula is related to the compound of saponins, flavonoids, and tannins. The results indicate that the yellow kepok banana corm extract has the potential to be developed as an anti-inflammatory drug.

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UJI EFEKTIVITAS SALEP EKSTRAK DAUN JARAK PAGAR (Jatropha curcas L.) TERHADAP PENYEMBUHAN LUKA SAYAT PADA TIKUS PUTIH JANTAN GALUR WISTAR (Rattus norvegicus)

PHARMACON ◽

10.35799/pha.9.2020.29283 ◽

2020 ◽

Vol 9 (2) ◽

pp. 284

Author(s):

Repatri A Bawotong ◽

Edwin De Queljoe ◽

Deby A Mpila

Keyword(s):

Wound Healing ◽

Jatropha Curcas ◽

Ethanol Extract ◽

Positive Control ◽

Negative Control ◽

Steroid Glycoside ◽

Treatment Groups ◽

Jatropha Curcas L ◽

White Rats ◽

Wistar Strain

ABSTRACTJatropha leaves (Jatropha curcas L.) contains alkaloid, saponin, tannin, terpenoid, steroid, glycoside, phenol compound and flavonoid through ethanol extract. Until today the community still used the plant as a wound healing medicine. The aim of this study is to examine the effectiveness of jatropha curcas L. leaf extract ointment for healing in male white rats wistar strain. Jatropha leaves extract ointment was tested on 5 which consists of 3 rats in each treatment groups, that is base ointment (negative control), ointment betadine (positive control), ointment extract of Jatropha leaves 10%, 20% and 40%. Observations has made until the 14th day. And data were tested using ANOVA (Analysis of Variants) and continued with LSD (Least Significant Different) test. Statistical test results showed a difference in the duration of wound healing in male white rats wistar strain between the ointment extract of jatropha leaf with ointment betadine and vaseline ointment. Keywords: Ointment, Jatropha extract (Jatropha curcas L.), Galur wistar, Wound.ABSTRAK Daun jarak pagar (Jatropha curcas L.) mengandung zat-zat alkaloid, saponin, tannin, terpenoid, steroid, glikosida, senyawa fenol dan flavonoid melalui ekstrak etanol. Hingga saat ini masyarakat masih menggunakan tanaman tersebut sebagai obat penyembuh luka. Penelitian ini bertujuan untuk menguji efektivitas salep ekstrak etanol daun jarak pagar terhadap penyembuhan luka sayat pada tikus putih jantan galur wistar. Salep ekstrak daun jarak pagar diujikan pada hewan uji tikus sebanyak 5 yang terdiri dari 3 ekor tikus disetiap kelompok perlakuan, yaitu basis salep (kontrol negatif), salep betadine (kontrol positif), salep ekstrak etanol daun jarak pagar 10%, 20% dan 40%. Pengamatan dilakukan sampai hari ke 14. Data diuji dengan menggunakan ANOVA (Analisis Of Variant) dan dilanjutkan dengan uji LSD (Least Significant Different). Hasil uji statistik menunjukan adanya perbedaan terhadap lamanya penyembuhan luka pada tikus putih jantan galur wistar antara sediaan salep ekstrak daun jarak pagar dengan salep salep betadine dan basis salep. Kata Kunci: Salep, Daun jarak pagar (Jatropha curcas L.), Galur wistar, Luka sayat.

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Efficacy of mengkudu leaves extract (morinda citrifolia) on wound healing rate post tooth extraction in white rats (rattus norvegicus)

Journal of Dentomaxillofacial Science ◽

10.15562/jdmfs.v3i1.547 ◽

2018 ◽

Vol 3 (1) ◽

pp. 28

Author(s):

Irfan Rasul ◽

Desy Setiady

Keyword(s):

Wound Healing ◽

Treatment Group ◽

Tooth Extraction ◽

Positive Control ◽

Negative Control ◽

Male Rats ◽

Positive Control Group ◽

Control Group ◽

Accelerate Wound Healing ◽

White Rats

Objective: This study aims to determine the efficacy of mengkudu leaves extract to accelerate wound healing post tooth extraction in white rats.Material and Methods: Laboratory experimental study was carried out using post-test only and control group design. This study was measured by observing the duration of wound healing post tooth extraction, starting from the formation of sockets after tooth extraction until the socket closed, with sample of 27 white rats in three study groups in accordance with inclusion and exclusion criteria that have been determined.Results: The average healing time in the negative control group is 19 days, the positive control group is 9 days, and the treatment group with mengkudu leaves extract is 7 days. Therefore, the fastest time of wound healing occurs in the treatment group, followed by the positive control group and lastly, in the negative control group.Conclusion: Mengkudu leaves accelerate wound healing post tooth extraction in white male rats.

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