scholarly journals Studies on Antibacterial Activity and Brine Shrimp Toxicity of Leaf Extract of Cassia grandis

2009 ◽  
Vol 3 (1) ◽  
pp. 17-19 ◽  
Author(s):  
MA Awal ◽  
Syed Ashrafuzzaman ◽  
E Ekramul Haque
Keyword(s):  
Antibacterial Activity ◽  
Brine Shrimp ◽  
Ethanol Extract ◽  
Salmonella Typhi ◽  
Diffusion Method ◽  
Gram Negative Bacteria ◽  
Disc Diffusion Method ◽  
Microbial Diseases ◽  
Lc50 Value ◽  
Bioassay Test

Antibacterial activity and toxicity of ethanol extract of Cassia grandis leaves to Brine Shrimp was evaluated. Five Gram-positive and 4 Gram-negative bacteria, namely Sarcina lutea, Bacillus megaterium, Bacillus subtilis, Streptococcus ß-haemolyticus, Staphylococcus aureus, Pseudomonas aeruginosa, Escherichia coli, Salmonella typhi and Klebsiella pneumoniae were tested using disc diffusion method. The extract was inactive at the concentration of 30 μg/disc but exhibited moderate to good activity at concentration of 200 μg/disc against the tested bacteria. In Brine Bhrimp lethality bioassay test, it was observed that LC50 value of the extract was 10.68 μg/ml. From these findings, it is indicative that C. grandis may be useful against microbial diseases. Key words: Antibacterial activity, Brine shrimp, Cassia grandis   doi: 10.3329/bjmm.v3i1.2966 Bangladesh J Med Microbiol 2009; 03 (01): 17-19

scholarly journals Aktivitas Antibakteri Ekstrak Etanol Daun Gatal (Laportea aestuans (L.) Chew)

2018 ◽  
Vol 9 (1) ◽  
pp. 1-7
Author(s):  
Eva S. Simaremare ◽  
Agustina Ruban ◽  
Dirk Y.P. Runtuboi
Keyword(s):  
Antibacterial Activity ◽  
Local Community ◽  
Ethanol Extract ◽  
Salmonella Typhi ◽  
The Body ◽  
Diffusion Method ◽  
Disc Diffusion Method ◽  
E Coli ◽  
Indigenous Plant ◽  
Made In

Daun gatal (Laportea aestuans) is an indigenous plant of Papua hich has been widely used for pain relief as traditional medication in the local community. The leaves were just picked then treated to cure for painful body. This treatment are giving itchy sense as an indication that medication is working on the body as local people assumed. The aim of the study was to determine the activity of antibacteria of daun gatal against Escherichia coli, Staphylococcus aureus and Salmonella typhi. Samples of daun gatal were collected from Biak Numfor Papua. The methods are started with filtering of daun gatal using 100 mash and extracted with ethanol. Extract was made in 250, 500, 750, 1000 ppm, with chloramphenicol as a control positive, while the test of antibacterial activity was done by disc diffusion method. The results showed that daun gatal has antibacterial activity against E. coli, S. aureus and S. typhi. Daun gatal extract (L. aestuans) is effective in inhibiting the growth of bacterias with zone inhibition of 1000 ppm extracts which were 8.55 mm (E. coli), 9.02 mm (S. typhi), dan 9.37 mm (S. aureus). Key words: Antibacteria, E. coli, L. aestuans, S. aureus and S. typhi.

scholarly journals Antibacterial Activity of Leaf Extracts ofBaeckea frutescensagainst Methicillin-ResistantStaphylococcus aureus

2014 ◽  
Vol 2014 ◽  
pp. 1-5 ◽  
Author(s):  
Somayeh Razmavar ◽  
Mahmood Ameen Abdulla ◽  
Salmah Binti Ismail ◽  
Pouya Hassandarvish
Keyword(s):  
Antibacterial Activity ◽  
Ethanol Extract ◽  
Diffusion Method ◽  
Disc Diffusion ◽  
Phytochemical Screening ◽  
Bioactive Constituents ◽  
Disc Diffusion Method ◽  
Leaf Extracts ◽  
Baeckea Frutescens ◽  
High Degree

This study was based on screening antibacterial activity of the ethanol extract ofBaeckea frutescensL. against MRSA clinical isolates, analyzes the potential antibacterial compound, and assesses the cytotoxicity effect of the extract in tissue culture. Leaves ofBaeckea frutescensL. were shade dried, powdered, and extracted using solvent ethanol. Preliminary phytochemical screening of the crude extracts revealed the presence of alkaloids, flavonoids, steroids, terpenoids, phenols, and carbohydrates. The presence of these bioactive constituents is related to the antibacterial activity of the plant. Disc diffusion method revealed a high degree of activity against microorganisms. The results confirm thatBaeckea frutescensL. can be used as a source of drugs to fight infections caused by susceptible bacteria.

scholarly journals Antibacterial Activity of Ethanol Extract of Spirulina platensis

2021 ◽  
Vol 7 (2) ◽  
pp. 47-51
Author(s):  
Prayoga Pannindrya ◽  
Mega Safithri ◽  
Kustiariyah Tarman
Keyword(s):  
Antibacterial Activity ◽  
Spirulina Platensis ◽  
Ethanol Extract ◽  
Positive Control ◽  
Diffusion Method ◽  
Test Results ◽  
Nutritional Content ◽  
Disc Diffusion Method ◽  
Antibacterial Test ◽  
E Coli

Spirulina is a microalgae that has been widely used as various supplements and medicines because of its high nutritional content. The need for new antibacterial sources to solve the problem of antibiotic resistance, makes the potential of Spirulina as an antibacterial agent necessary to be researched. This study aims to analyze the antibacterial activity of ethanol extract of Spirulina platensis obtained from the Indonesian region against Staphylococcus aureus and Escherichia coli. Spirulina samples were macerated in ethanol solvent in a ratio of 1:10 (w / v). The antibacterial test used was the disc diffusion method with clindamycin positive control. Antibacterial test results showed that S. platensis in this study did not have antibacterial activity against E. coli and S. aureus.

scholarly journals A Study on Antibacterial Activity of Silver Nanoparticles

2018 ◽  
Vol 29 (2) ◽  
pp. 37-41
Author(s):  
M Iqbal Hossain ◽  
M Anwar Habib ◽  
Nazimuddin Ahmed
Keyword(s):  
Silver Nanoparticles ◽  
Antibacterial Activity ◽  
Antibacterial Effect ◽  
Diffusion Method ◽  
Gram Negative Bacteria ◽  
Disc Diffusion ◽  
Disc Diffusion Method ◽  
Reference Drug ◽  
Gram Negative ◽  
Quasi Experimental

This quasi experimental study was designed to evaluate antibacterial activity of chemically synthesized silver nanoparticles (AgNPs) from silver nitrate (AgNO3) solutions on gram negative bacteria like E.Coli using disc diffusion method. Different concentrations of AgNPs, AgNO3 and reference drug ciprofloxacin were used to find out the antibacterial activity which revealed that AgNPs possessed significant antibacterial effect compared to AgNO3 solutions but relatively less antibacterial effect than that of ciprofloxacin. So chemical synthesis guided AgNPs may have some antibacterial effects.TAJ 2016; 29(2): 37-41

scholarly journals New Diarylheptanoid from Garuga pinnata Roxb

2013 ◽  
Vol 61 (2) ◽  
pp. 131-134 ◽  
Author(s):  
Mst Taslima Khatun ◽  
M. Mahboob Ali Siddiqi ◽  
Al-Mansur MA ◽  
MH Sohrab ◽  
AFM Mustafizur Rahman ◽  
...  
Keyword(s):  
Brine Shrimp ◽  
Microbial Growth ◽  
Stem Bark ◽  
Diffusion Method ◽  
Gram Negative Bacteria ◽  
Disc Diffusion ◽  
Disc Diffusion Method ◽  
Gram Negative ◽  
Wide Range ◽  
Bacteria And Fungi

9´-Desmethylgaruganin I has been isolated from the dichloromethane extract of the stem bark of Garuga pinnata Roxb. The crude extract was screened for antimicrobial activity against a wide range of gram-positive and gram-negative bacteria and fungi by disc diffusion method and cytotoxicity by brine shrimp lethality bioassay. The dichloromethane extract showed moderate inhibitory activity to microbial growth and weak cytotoxicity having LC90 25.703?g-mL–1 DOI: http://dx.doi.org/10.3329/dujs.v61i2.17058 Dhaka Univ. J. Sci. 61(2): 131-134, 2013 (July)

scholarly journals Synthesis and in vitro antibacterial activity of Cephradine Benzoate

1970 ◽  
Vol 10 (3) ◽  
pp. 148-151
Author(s):  
M Ahmad ◽  
M Rahman ◽  
P Kumar Paul
Keyword(s):  
Antibacterial Activity ◽  
Salmonella Typhi ◽  
Shigella Dysenteriae ◽  
Diffusion Method ◽  
Disc Diffusion ◽  
Disc Diffusion Method ◽  
Nmr Studies ◽  
Shigella Boydii ◽  
Group B

Aims & Methods: The present study was undertaken to compare the antibacterial activity of a cephradine derivative with that of the parent antibiotic cephradine. Cephradine was converted to its benzoyl derivative by Schotten-Baumann method for the first time. Disc diffusion method was employed to find out the antibacterial activity against EPEC, ETEC, E. Agg, Salmonella typhi, Salmonella group B, Shigella boydii, Shigella dysenteriae 1, Shigella dysenteriae 2, Shigella flexinariae and Shigella sonnei. Melting point, TLC, HPLC, UV, FTIR and <sup>1</sup>H NMR studies were carried out to check the purity and confirm that the derivative was cephradine benzoate. Results: The benzoyl derivative showed promising activity against tested bacteria. The results obtained from the study demonstrate that the benzoyl derivative could be a potential antibacterial agent. Key words: Cephradine benzoate, antibacterial activity, disc diffusion method.   DOI: http://dx.doi.org/ 10.3329/bjms.v10i3.8356 BJMS 2011; 10(3): 148-151

scholarly journals Skrining Aktivitas Antibakteri Ekstrak Etanol Daun Terhadap Salmonella Typhi Resisten Kloramfenikol

2017 ◽  
Vol 2 (02) ◽  
pp. 66
Author(s):  
Ika Trisharyanti
Keyword(s):  
Antibacterial Activity ◽  
Inhibitory Concentration ◽  
Minimum Bactericidal Concentration ◽  
Ethanol Extract ◽  
Salmonella Typhi ◽  
Diffusion Method ◽  
Dilution Method ◽  
Tea Leaves ◽  
Disk Diffusion Method ◽  
Phaleria Macrocarpa

<em><span lang="EN-US">Typhoid fever is an infectious disease caused by Salmonella typhi, antibiotic chloramphenicol can be use to treatment, but S. typhi resistant with antibiotic choramphenicol so need to alternative treatment. The purpose of this study was to determine the antibacterial activity of ethanol extract of ten leaves against Salmonella typhi, and knowing the compounds contained in extracts of leaves that have the best antibacterial activity. The leaves was extracted with  ethanol 96% by maceration method. Screening antibacterial activity used disk diffusion method with 10% extract concentration. The best antibacterial activity was determined Minimum Inhibitory Concentration and Minimum Bactericidal Concentration used liquid dilution method, identification test of compounds with thin-layer chromatograpy (TLC), and bioautografi test. There were six extracts had antibacterial activity against S. Typhi, Phaleria macrocarpa leaves, Acalypha siamensis leaves, cherry leaves, clove leaves, tea leaves, and Eugenia polyantha leaves. Minimum Bactericidal Concentration from clove leaf extract was 2,5%. The results of identification by TLC compounds were alkaloids, phenolics, flavonoids, terpenoids, triterpenoids, and saponins. Based on bioautografi test,  phenolic can inhibit the growth of Salmonella typhi.</span></em>

scholarly journals Isolation of bacteria from Apis cerana hive, their antibacterial potency and cytotoxicity

2019 ◽  
Vol 20 (9) ◽  
Author(s):  
YEKKI YASMIN ◽  
Lenni Fitri ◽  
FAUZIAH ◽  
FITRI WASLIYAH
Keyword(s):  
Antibacterial Activity ◽  
Toxicity Test ◽  
Apis Cerana ◽  
Ethanol Extract ◽  
Probit Analysis ◽  
Diffusion Method ◽  
Disk Diffusion Method ◽  
Bacterial Extract ◽  
Lc50 Value ◽  
Antibacterial Assay

Abstract. Yasmin Y, Fitri L, Fauziah, Wasliyah F. 2019. Isolation of bacteria from Apis cerana hive, their antibacterial potency and cytotoxicity. Biodiversitas 20: 2733-2738. This study aimed to identify bacteria from Apis cerana hive; to determine their antibacterial activity, and cytotoxic effect of hive extract as well as bacterial extract. Identification of bacteria isolated from the hive was carried out based on morphological and biochemical characters. The antibacterial assay of beehive bacteria isolates was done by disk diffusion method against Staphylococcus aureus and Escherichia coli. Toxicity test of beehive extract and beehive bacterial extract were carried out using Brine Shrimp Lethality Test (BSLT) with six final concentrations (32 ppm, 64 ppm, 126 ppm, 250 ppm, 500 ppm, and 1000 ppm). The LC50 value was determined by probit analysis using SPSS. Bacterial isolation showed there were four bacterial isolates from A. cerana hive were the genus Bacillus (BSL1, and BSL3), the genus Micrococcus (BSL2) and the genus Neisseria (BSL4). The antibacterial assay showed that BSL4 isolate has the highest antibacterial activity against S. aureus with the diameter of the inhibitory zone was 32.6 mm. Toxicity test showed that A. cerana hive extract and bacterial ethanol extract from A. cerana hive had LC50 value of 67,744 ppm and 86.985 ppm respectively and categorized as toxic.

scholarly journals UJI AKTIVITAS ANTIBAKTERI EKSTRAK ETANOL BAWANG DAYAK (Eleutherine americana Merr) TERHADAP BAKTERI Staphylococcus aureus, Escherichia coli DAN Salmonella typhi

PHARMACON ◽  
2020 ◽  
Vol 9 (4) ◽  
pp. 525
Author(s):  
Rafi’a Adinda Putri ◽  
Herny E.I. Simbala ◽  
Deby A. Mpila
Keyword(s):  
Antibacterial Activity ◽  
Ethanol Extract ◽  
Inhibition Zone ◽  
Salmonella Typhi ◽  
Diffusion Method ◽  
Disk Diffusion Method ◽  
E Coli ◽  
Activity Test ◽  
Paper Disk ◽  
Eleutherine Americana

ABSTRACTDayak onions (Eleutherine americana Merr) has been used as a traditional plant which has  benefits  for  many  diseases,  one  of  them  is  antibacterial. This study aims to determine the growth inhibition of ethanol extract of Dayak onions leaves on the bacterium S. aureus, E. coli and  S. typhi using three concentrations of 20%, 40% and 60%. Extraction was done by maceration method using ethanol 96%. Antibacterial activity test was performing using the paper disk diffusion method (Kirby-Bauer diffusion). The results showed that ethanol extract of bawang dayak has the potential as an antibacterial and has antibacterial strength against S. aureus, E. coli and S. typhi. At concentrations of 20%, 40% and 60% forming inhibition zone diameters (16.23 mm, 19.18 mm, 21.25 mm) S. aureus, (10.7 mm, 13.98 mm, 15.87 mm) E. coli, and  (14.43 mm, 14.6 mm, 17.2 mm) S. typhi. At concentration of 20%, 40% and 60% included strong groups to inhibit E. coli and  S. typhi, while at concentration of 60%, it is included in a very strong group to inhibit the S. aureus bacteria. Keywords: Antibacterial activity, Bawang Dayak , Kirby-Bauer diffusion method   ABSTRAK Bawang Dayak (Eleutherine americana Merr) telah digunakan sebagai tanaman tradisional yang bermanfaat bagi banyak penyakit salah satunya sebagai antibakteri. Penelitian ini bertujuan untuk mengetahui ekstrak etanol bawang dayak terhadap bakteri S. aureus, E.coli dan S. typhi menggunakan tiga kosentrasi yaitu 20%, 40% dan 60%. Ekstraksi dilakukan dengan metode maserasi menggunakan etanol 96%. Pengujian aktivitas antibakteri dilakukan dengan  menggunkan metode difusi dengan cakram kertas (difusi Kirby-Bauer). Hasil penelitian menunjukkan bahwa ekstrak etanol bawang dayak berpotensi sebagai antibakteri dan memiliki kekuatan antibakteri terhadap S. aureus, E. coli dan S. typhi. Pada konsentrasi 20%, 40% dan 60% membentuk diameter zona hambat (16.23 mm, 19.18 mm, 21.25 mm) pada S. aureus, (10.7 mm, 13.98 mm, 15,87 mm) pada E. coli, (14.43 mm, 14.6 mm, 17.2 mm) pada S. typhi. Pada konsentrasi 20%, 40% dan 60% termasuk golongan kuat untuk menghambat bakteri E. coli dan S. typhi, sedangkan pada konsentrasi 60% termasuk dalam golongan sangat kuat untuk menghambat bakteri S. aureus. Kata kunci: Aktivitas antibakteri, Bawang Dayak, metode difusi Kirby-Bauer

scholarly journals Antibacterial activity test of Simargaolgaol (Aglaonema modestum Schott ex Engl) leaves extract against Escherichia coli and Salmonella typhi bacteria

2021 ◽  
Vol 13 (2) ◽  
pp. 151-158
Author(s):  
Tiurma Solomasi Zega ◽  
◽  
Putri Mandaoni Pakpahan ◽  
Rahmayani Siregar ◽  
Givinda Sitompul ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Antibacterial Activity ◽  
Ethyl Acetate ◽  
Leaf Extract ◽  
Ethanol Extract ◽  
Salmonella Typhi ◽  
Diffusion Method ◽  
Activity Test ◽  
Inhibitory Power ◽  
Ethanol Extracts

The Simargaolgaol (Aglaonema modestum Schott ex Engl) plant is a plant that grows wild in Barus District, Central Tapanuli, North Sumatra which is used by the community as a medicinal plant. This study aims to determine the antibacterial activity of n-hexane, ethyl acetate and ethanol extracts from Simargaolgaol leaves against Escherichia coli and Salmonella typhi bacteria. The antibacterial activity test of Simargaolgaol leaves was carried out using the disc diffusion method. The results of antibacterial activity showed that the highest inhibitory power of Simargaolgaol leaf extract was ethanol extract (polar), ethyl acetate extract (semi polar) and n-hexane extract (non polar). The inhibitory power of ethanol extract, ethyl acetate, and n-hexane against Escherichia coli bacteria was 13.1 mm (strong); 9.7 mm (medium); 8.0 mm (medium) and for Salmonella typhi bacteria respectively 11.2 mm (strong); 10.7 mm (strong) and 9.3 mm (medium). With the concentration of the extract in the diameter is 10%. Based on this, it can be concluded that Simargaolgaol leaf extract has potential as an antibacterial. Keywords: Aglaonema modestum Schott ex Engl, Antibacterial activity, Escherichia coli, Salmonella typhi

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