scholarly journals Biodegradable Zinc Oxide Nanoparticles Doped with Iron as Carriers of Exogenous Iron in the Living Organism

2021 ◽  
Vol 14 (9) ◽  
pp. 859
Author(s):  
Paula Kiełbik ◽  
Aneta Jończy ◽  
Jarosław Kaszewski ◽  
Mikołaj Gralak ◽  
Julita Rosowska ◽  
...  
Keyword(s):  
Hematological Parameters ◽  
Iron Concentration ◽  
Living Organism ◽  
Blood Parameters ◽  
The Body ◽  
Zno Nanoparticle ◽  
Hepcidin Expression ◽  
Adult Mice ◽  
Tissue Iron ◽  
Average Size

Iron plays an important role in various crucial processes in the body and its deficiency is considered currently as a serious health problem. Thus, iron supplementation strategies for both humans and animals need to be effective and safe. According to our previous studies, zinc-based nanoparticles provide safe, biodegradable, fast and efficient transport system of orally given substances to the tissues. In the current manuscript we present results of a study aimed at investigation of the ZnO nanoparticle-based Fe supplementation system (average size 100 × 250 nm). Nanostructures were orally (gavage) administered to adult mice. Animals were sacrificed at different time points with collection of blood and internal organs for analyses (tissue iron concentration, hepatic level of hepcidin, blood parameters, liver and spleen levels of ferritin, histopathology). Initial experiment was performed to compare the biological effect of doping type (Fe3+ doping vs. a mixture of Fe3+ and Fe2+). Then, the effect of acute/chronic exposure models was determined. The increase in ferritin, along with improved, crucial hematological parameters and lack of the influence on hepcidin expression indicated the chronic application of Fe3+,2+ doped ZnO nanostructures to be the most effective among tested.

scholarly journals The effect of Ricinus communis seeds extract on reproductive activity and blood values of male rabbits

2019 ◽  
Vol 12 (1) ◽  
pp. 97-106
Author(s):  
AL-Khafaji Nazar
Keyword(s):  
Estrogenic Activity ◽  
Ricinus Communis ◽  
Hematological Parameters ◽  
Abdominal Cavity ◽  
Hemoglobin Concentration ◽  
Reproductive Activity ◽  
Treated Group ◽  
Blood Parameters ◽  
The Body ◽  
Tropical Regions

Ricinus communis L. of Euphorbiaceae family is a widespread plant in tropical regions. It is used in traditional medicines as an anti- fertility agent in India and different parts of the world. The ether soluble portion of the methanol extract of R. communis var minor possesses anti-implantation, anti - conceptive and estrogenic activity in rats and mice when administered subcutaneously.The study was conducted on 10 local breed male rabbits, 1-2 years old, of 1-2 kg body weight. The animals were divided into two groups, control non – treated group and treated group in which animals were treated with single daily dose of 50 mg /kg b. wt. P.O. of decorticated and defatted castor seeds (DDCS) for 14 days. 28th day post treatment, animals were anesthetized by diethyl ether, sacrificed, abdominal cavity was open. The sexual organ (testes, epididymis, prostate and seminal vesical) weighed. In addition to take a biopsy from each one for histopathological changes. The study also included clinical and hematological parameters, in addition to sperm counts and the changes in sperm morphology.Body weight, body temperature increased significantly in treated males. While in non- treated group there were no significant changes. Respiratory rates and heart rate were none significantly changed in treated and non- treated males.Bleeding time none significantly increased in treated males, but increased significantly in none treated males. Clotting times decreased none significantly in treated and non- treated males. The blood parameters including, total erythrocytes count, hemoglobin concentration, PCV% , MCV, MCH, MCHC, total leucocyte and differential leucocyte counts were either increased or decreased none significantly in both groups. The results revealed that the effects of exposure to extract of ricin for 14 days on reproductive efficiency of rabbits, exhibited Significant decrease in weights of testes, epididymis, tails, heads of epididymis, seminal vesicles and prostate in treated males in comparison with those of non- treated males. While the body of epididymis did not show a significant changes.Significant decrease in live sperm numbers, number of sperms in epididymal head, in addition to deformities in high numbers of sperm, including enlarged or small sperms. breaks head, and its detachment, presence of two heads in one sperm, bifurcation of tail and its breaking, sperm coiling in samples from treated males in comparison with those from non-treated males.Histological changes were hyperplasia of lining epithelial cells and vacuolar degenerative changes, loss of spermatogenesis, and spermatocytes necrosis in those from treated males.

scholarly journals Effects of Imidacloprid-Contaminated Feed Exposure on Hematological Parameters in Adult Rabbits (Oryctolagus Cuniculus)

2020 ◽  
Vol 7 (3) ◽  
pp. 439-444
Author(s):  
Md Alamgir Kobir ◽  
Latifa Akther ◽  
Imam Hasan ◽  
Md Ahosanul Haque Shahid ◽  
Ziaul Haque ◽  
...  
Keyword(s):  
Leukocyte Count ◽  
Hematological Parameters ◽  
Blood Parameters ◽  
The Body ◽  
Standard Diet ◽  
Cell Analysis ◽  
Differential Leukocyte Count ◽  
Once Daily ◽  
Important Index ◽  
Fresh Vegetables

Blood parameters are an important index to assess the body homeostasis in mammals. Here, the effect of pesticide (imidacloprid) contaminated feed exposure on the haematological parameters of adult rabbits (n=15) was studied. Pesticides (Imidacloprid, Bildor® 0.5ml (100mg)/L water spray on vegetables and green grass) exposed feed fed to rabbits with wash (washed feed rabbit) or without a wash (not washed feed rabbit) in the once-daily morning for every alternative day up to 15 days. Control rabbits have received a standard diet (fresh vegetables and green grass). The blood cell analysis showed that the total erythrocyte count, packed cell volume, and haemoglobin values were not changed significantly in pesticide exposed rabbit. For differential leukocyte count, the percentage of neutrophil and eosinophil was significantly decreased in the pesticide-exposed rabbit as compared to the control rabbit. The lymphocyte percentage was increased significantly in pesticide-exposed rabbits compared to control rabbits. Interestingly, the number of lymphocytes was significantly increased in not washed feed rabbits compared to wash feed rabbits. The results of the present study suggest that residue of agriculturally used pesticides may affect the body homeostasis of animals. Res. Agric., Livest. Fish.7(3): 439-444,  December 2020

Hematological Indicators of Moose in Domestication Process

2020 ◽  
Keyword(s):  
Hematological Parameters ◽  
Physiological State ◽  
Blood Parameters ◽  
The Body ◽  
Sex Characteristics ◽  
Hematological Indices ◽  
Blood Indices ◽  
Hematological Indicators ◽  
Age And Sex ◽  
Selection Of

Presented hematological indices for a stress-free selection of material from moose in the process of domestication depending on age and sex characteristics. Fluctuations in the blood parameters of moose have been established within various limits, taking into account the influence of various factors - the climatic, environmental, technological, physiological state of the animal, and age and sex characteristics. Considerable attention is paid to the selection of blood from moose, one of the most labor-intensive processes when working with them. Some tendency to fluctuations in hematological parameters in young moose has been recorded. This, possibly, is associated with a decrease in the action of colostral immunity, the formation of a specific defense of the body. Differences in blood indices in moose depending on gender were unreliable. However, in males, some of them - in terms of the content of erythrocytes, basophils, and monocytes - was higher than in females, and vice versa, in females - the number of leukocytes, lymphocytes, and eosinophils. It was shown that in young animals of 21-50 days of age, a neutrophilic profile prevails with increased content of segmented forms, and in adults - a lymphocytic profile. Compared with moose, which are in the process of domestication in animals living in natural conditions, expressed leukocytosis was recorded. As a result of the studies, the estimated norms of morphological parameters of the blood of moose in the process of domestication are established, depending on age and gender.

Erythroferrone Regulates Hepcidin Expression Independently of Matriptase 2

Blood ◽  
2016 ◽  
Vol 128 (22) ◽  
pp. 3616-3616 ◽  
Author(s):  
Leon Kautz ◽  
Sharraya Aschemeyer ◽  
Victoria Gabayan ◽  
Tomas Ganz ◽  
Elizabeta Nemeth
Keyword(s):  
Limit Of Detection ◽  
Hematological Parameters ◽  
Isolated Hepatocytes ◽  
Bmp Signaling ◽  
The Body ◽  
Deficiency Anemia ◽  
Wild Type ◽  
Hepcidin Expression ◽  
Undetectable Plasma ◽  
Transmembrane Serine Protease

Abstract Introduction: The iron-regulatory hormone hepcidin regulates the body iron stores and its expression is repressed when erythropoietic activity intensifies to meet the iron requirements for erythropoiesis (e.g. during anemia). Under the influence of erythropoietin (EPO), the hormone erythroferrone (ERFE) is secreted by erythroid precursors in the bone marrow and the spleen, and suppresses hepcidin synthesis to facilitate the recovery from anemia. However, the mechanism by which ERFE suppresses hepcidin is unknown. In contrast with forms of anemia in which hepcidin is suppressed, patients with mutations in transmembrane serine protease 6 (TMPRSS6) have iron-refractory iron deficiency anemia (IRIDA) but increased hepcidin production despite a severe anemia and elevated EPO levels. Recently, it has been suggested that matriptase-2 activity facilitates ERFE-mediated suppression of hepcidin. We therefore investigated the potential crosstalk between ERFE and Matriptase 2. Methods: We first measured serum ERFE concentration in Tmprss6-/- mice. To assess the contribution of ERFE to the phenotype of Tmprss6-/-mice, we next generated Tmprss6-/-mice with disrupted Erfe (Erfe+/- Tmprss6-/-; Erfe-/- Tmprss6-/- and Erfe+/+ Tmprss6-/-). To determine whether ERFE requires TMPRSS6 to regulate hepcidin production, we treated freshly isolated hepatocytes from wild-type (WT) or Tmprss6-/- mice with conditioned medium from cells expressing recombinant ERFE or not. Results: While wild-type mice have undetectable plasma ERFE (below the 500 pg/ml limit of detection), plasma ERFE concentration was elevated in Tmprss6-/- to levels comparable to those of WT animals 24 hours after phlebotomy (~3 ng/ml) but was lower than ERFE levels in thalassemic mice (~10 ng/ml). Ablation of Erfe in Tmprss6-/- mice did not result in any change in hematological parameters, hepcidin expression and iron levels compared to Tmprss6-/- animals at 6 weeks of age. However, treatment of WT and Tmprss6-/-hepatocytes with ERFE resulted in a comparable suppression of hepcidin mRNA expression. Conclusion: Although matriptase-2 may dampen the BMP signaling under the influence of EPO, it is not part of the ERFE signaling pathway. Disclosures Ganz: Intrinsic Lifesciences: Other: shareholder and scientific advisor; Merganser Biotech: Other: shareholder and scientific advisor; Silarus therapeutics: Other: shareholder and scientific advisor; Keryx Biopharmaceuticals: Consultancy. Nemeth:Intrinsic Lifesciences: Other: shareholder and scientific advisor; Merganser Biotech: Other: shareholder and scientific advisor; Silarus therapeutics: Other: shareholder and scientific advisor.

scholarly journals Selected blood parameters altered by different doses of pesticide Malathion in albino rat (Rattus-norvegicus)

2020 ◽  
Vol 21 (3) ◽  
pp. 93-103
Author(s):  
C.D. Sharma ◽  
Neha Shukla ◽  
Geeta Bansal
Keyword(s):  
Body Weight ◽  
Hematological Parameters ◽  
Blood Parameters ◽  
Control Agent ◽  
The Body ◽  
Hematological Parameter ◽  
Albino Rats ◽  
Albino Rat ◽  
Toxicological Effects ◽  
Different Doses

Organophosphate pesticides Malathion used as pest control agent in agriculture, household, kitchen and other places. Malathion is found in market with different trade names. It is used with its action properties against various insects and pest. It is used with the largest group of poisonous substances that are widely broadcast today. Present study is based on the effects of different doses of Malathion on routine hematological parameters like RBCs, WBCs, Hb%, CT, PT, ESR, PCV, MCV, MCH, and MCHC. Four doses were selected according to the body weight of albino rats. The doses were selected as four types on the basis of per kilogram body weight of albino rat. They were 25, 50, 75,100 mg per Kg body weight for 7 and 15 days of treatment.The findings indicate that the above parameter fluctuates significantly as amount of dose increases. Hematological parameter indicates that the total body of the animals gets affected by the Malathion. It causes various other toxicological effects on the body of albino rats. There was a combined study of male and female albino rats.

scholarly journals Ecotoxical influence of plumbum and cadmіum on gematological parameters of sylver carp (hypopthalmichtys molitrix)

2017 ◽  
Vol 19 (74) ◽  
pp. 24-28
Author(s):  
N. I. Hradovych
Keyword(s):  
Heavy Metals ◽  
Hematological Parameters ◽  
Silver Carp ◽  
Ecological Factors ◽  
Blood Parameters ◽  
The Body ◽  
Control Group ◽  
Research Station ◽  
Hematological Indices ◽  
Experimental Part

Many authors noticed the actuality of fish hematological parameters usage as indices of anthropogenic influence. Blood system reflects the reaction of the body on the action of different ecological factors. The problem of heavy metals influence on hematological parameters of silver cap (Hypopthalmichtys molitrix) needs to be investigated. The aim of the work was studying of ecotoxical Plumbum and Cadmium influence on hematological indices of silver carp (Hypopthalmichtys molitrix) during the period exposition. The results of the investigations concerning the influence of Plumbum and Cadmium on some hematological indices of silver carp (Hypopthalmichtys molitrix) for 12 days exposition up to the action of these heavy metals are given in this article. Experimental part was realized at the Lviv research station of Institute of fisheries NAAS (Lviv oblast, Horodok region, Velykyi Lyubin). Experimental groups contained 10 two-years individuals each. Fish were adopted to laboratory conditions for 5 days under the temperature 19 ± 2ºС, рН = 7.3 ± 0.2, oxygen content 7–8 mg/l. Blood parameters were measured on the sixth and twelfth days at the control group and groups which were under the influence on the level of 2 and 5 MPC of the appropriate metal. It was proved that the influence of Plumbum and Cadmium causes the lowering of erythrocytes number, hemoglobin and corpuscular volume: the effect becomes stronger when he concentration increases and in time. The effect from intoxication by Cadmium comes faster than by Plumbum.

scholarly journals Antioxidant Effect of Saccharomyces cerevisiae Enriched with Selenium Against Pseudomonas aeruginosa Infection

2021 ◽  
Vol In Press (In Press) ◽  
Author(s):  
Khadijeh Mansouri-Habibabadi ◽  
Mahnoosh Fatemi ◽  
Fereshteh Ghandehari
Keyword(s):  
Oxidative Stress ◽  
Saccharomyces Cerevisiae ◽  
Pseudomonas Aeruginosa ◽  
Hematological Parameters ◽  
White Blood Cells ◽  
Blood Parameters ◽  
The Body ◽  
Control Group ◽  
Antioxidant Agents ◽  
Total Antioxidant

Background: The reaction of immune cells to the introduction of pathogens into the body is an increase in reactive oxygen species, which leads to the induction of oxidative stress. Reducing the level of oxidative stress through the use of antioxidants strengthens the immune system. Objectives: This study aimed to investigate the antioxidant effects of Saccharomyces cerevisiae and selenium-enriched S. cerevisiae against the oxidative stress induced by Pseudomonas aeruginosa in rats. Methods: A total of 36 rats were randomly divided into six groups, including control (A), S. cerevisiae (B), S. cerevisiae enriched with selenium (C), P. aeruginosa infection treated with S. cerevisiae (D), P. aeruginosa infection treated with S. cerevisiae enriched with selenium (E), and P. aeruginosa infection (F). At the end of the treatment period, the blood samples were collected to evaluate hematological parameters, total antioxidant capacity, glutathione concentration, and glutathione peroxidase activity. Results: The concentration and activity of antioxidant agents in group F were significantly decreased compared to those of the control group; however, in groups D and E, the levels of the aforementioned factors showed a significant increase compared to those of the infected group. The number of white blood cells in group F showed a significant increase, compared to that of the control group. In other groups, the observed differences in antioxidant factors and blood parameters were not significant, compared to those reported for the control group. Conclusions: The results showed that the induction of oxidative stress by P. aeruginosa was partially reduced by treatment with yeast and selenium-enriched yeast. Further studies are essential to confirm the aforementioned results.

scholarly journals Hematological indexes of dogs at dirofilariosis

2020 ◽  
pp. 77-84
Author(s):  
D. Kryvoruchenko ◽  
Y. Prykhodko ◽  
G. Vikulina ◽  
O. Mazannyi
Keyword(s):  
Hematological Parameters ◽  
Vascular Wall ◽  
Final Diagnosis ◽  
Blood Parameters ◽  
The Body ◽  
Laboratory Diagnostics ◽  
Heartworm Disease ◽  
Inflammatory Processes ◽  
And Migration ◽  
Intensity Of Invasion

Laboratory diagnostics for any animal pathology refers to special research methods and helps not only in establishing the final diagnosis, but also in expanding data on the course of primary pathology and determining secondary complications based on changes in blood parameters. For this purpose, a clinical study of the blood of dogs with varying degrees of intensity of dirofilariasis invasion was carried out. It was found that the number of erythrocytes decreased in animals of the experimental groups, depending on the intensity of invasion. Severe erythropenia was found in the group of animals with the highest degree of invasion intensity (2.04±0.50 1012/l (p<0.001). It increased with an increasing in the degree of invasion and ESR up to 40.28±12.67 mm/h (p<0.05) and 62.50±14.16 mm/h (p<0.01). Thrombocyto-penia at 199.13±55.31 109/l was found in dogs in the group with the highest degree of invasion. Oligochromemia in animals also depended on the intensity of invasion and was 94.44±12.30 g/l (p<0.05) and 52.00±12.69 g/l (p<0.001). In dogs with the highest number of dirofilaria larvae, the hematocrit was 19.75±6.34 % (p<0.01) and leukocytosis developed (20.53±8.91 109/l). By calculating the leukocyte count in dogs with the highest degree of dirofilariasis invasion, a significant increase in the number of segmented neu-trophils was found to 86.00±10.43 % (p<0.1), eosinophils up to 27.88±5.54 % (p<0.001), basophils up to 1.75±1.39 % (p<0.001), appeared in blood of sick animals young neutro-phils (0.50±1.07 %, p<0.001). The established changes in hematological parameters are associated with the development of hemolytic anemia during invasion and the presence of latent bleeding associated with the destruction of the vascular wall by dirofilariae. The relative leukocytosis, which were diagnosed in sick dogs, is associated with the presence of nematodes and the development of inflammatory processes at the sites of localization and migration of dirofilaria. It is possible that leukocytosis and a shift of the leukocyte formula to the left are associated with intoxication of the body. Key words: dogs, heartworm disease, Dirofilaria im-mitis, blood, erythrocytes, leukocytes, leukocyte formula, hemoglobin, hematocrit, erythrocyte sedimentation rate.

Erythrocyte and reticulocyte parameters during biological monitoring of lead exposure to the body

2020 ◽  
Vol 60 (11) ◽  
pp. 873-876
Author(s):  
Anastasiya G. Khotuleva ◽  
Mariya S. Kozyreva
Keyword(s):  
Biological Monitoring ◽  
Hematological Parameters ◽  
Lead Level ◽  
Statistical Processing ◽  
Absorption Spectrometry ◽  
The Body ◽  
Control Group ◽  
Processing Plant ◽  
Hematological Disorders ◽  
Globin Synthesis

Introduction. The most susceptible to lead is the hematopoietic system of hematopoietic organs due to lead inhibition of heme and globin synthesis and cytotoxic effect on the membrane of Mature red blood cells. The aim of study was to evaluate the informative value of the study of erythrocyte and reticulocyte parameters determined on modern hematological analyzers in patients working in contact with lead during medical and biological monitoring. Materials and methods. 45 employees of the lead battery processing plant and 30 persons of control group were examined. The level of lead in the blood was determined by atomic absorption spectrometry, δ-ALA in the urine-by the reaction of pyrol formation with acetylacetone in terms of gram of creatinine, the study of hematological parameters was performed on a Sysmex HT-2000i analyzer. Statistical processing of the results was performed using the program STATISTICA 10.0. Results. Significant changes in erythrocytic (RDW) and reticulocytic (RET, IRF, LFR, MFR, HFR, RET-He) parameters, erythropoietin in workers in contact with lead compared to the control group, changes in MCV, MCH, RDW, RET indicators in the group working in dynamics after 2 years were revealed. Associations of hematological parameters with biomarkers of exposure and effect (lead level in blood and ALA in urine) were revealed. Conclusions. Assessment of erythrocyte (MCV, MCH, RDW) and reticulocyte parameters (RET% and their distribution by maturity) in dynamics during periodic medical examinations of workers in contact with lead allows us to detect the development of hematological disorders at early stages.

scholarly journals Effect of Chronic Administration of 5-(3-chlorophenyl)-4-Hexyl-2,4 -Dihydro-3H-1,2,4-Triazole-3-Thione (TP-315)—A New Anticonvulsant Drug Candidate—On Living Organisms

2021 ◽  
Vol 22 (7) ◽  
pp. 3358
Author(s):  
Anna Makuch-Kocka ◽  
Marta Andres-Mach ◽  
Mirosław Zagaja ◽  
Anna Śmiech ◽  
Magdalena Pizoń ◽  
...  
Keyword(s):  
Hematological Parameters ◽  
Anticonvulsant Drug ◽  
Living Organism ◽  
Drug Candidate ◽  
In Vitro Tests ◽  
Maximal Electroshock ◽  
Tp 315 ◽  
The Impact ◽  
Chronic Use

About 70 million people suffer from epilepsy—a chronic neurodegenerative disease. In most cases, the cause of the disease is unknown, but epilepsy can also develop as the result of a stroke, trauma to the brain, or the use of psychotropic substances. The treatment of epilepsy is mainly based on the administration of anticonvulsants, which the patient must most often use throughout their life. Despite significant progress in research on antiepileptic drugs, about 30% of patients still have drug-resistant epilepsy, which is insensitive to pharmacotherapy used so far. In our recent studies, we have shown that 4-alkyl-5-aryl-1,2,4-triazole-3-thiones act on the voltage-gated sodium channels and exhibit anticonvulsant activity in an MES (maximal electroshock-induced seizure) and 6Hz test in mice. Previous studies have shown their beneficial toxic and pharmacological profile, but their effect on a living organism during chronic use is still unknown. In the presented study, on the basis of the previously conducted tests and the PAMPA (parallel artificial membrane permeability assay) BBB (blood–brain barrier) test, we selected one 1,2,4-triazole-3-thione derivative—TP-315—for further studies aimed at assessing the impact of its chronic use on a living organism. After long-term administration of TP-315 to Albino Swiss mice, its effect on the functional parameters of internal organs was assessed by performing biochemical, morphological, and histopathological examinations. It was also determined whether the tested compound inhibits selected isoforms of the CYP450 enzyme system. On the basis of the conducted tests, it was found that TP-315 does not show nephrotoxic nor hepatotoxic effects and does not cause changes in hematological parameters. In vitro tests showed that TP-315 did not inhibit CYP2B6, CYP2D6, CYP3A4, or CYP3A5 enzymes at the concentration found in the serum of mice subjected to long-term exposure to this compound.

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