scholarly journals Efek Ekstrak Etanol Daging Putih Semangka dan Simvastatin Terhadap Aktivasi Nuclear Factor Kappa Beta (NF- kβ) Aorta Tikus Rattus Norvegicus yang Diberi Diet Aterogenik

2018 ◽  
Vol 11 (2) ◽  
Author(s):  
Dafrosia Darmi Manggasa
Keyword(s):  
Rattus Norvegicus ◽  
Atherogenic Diet ◽  
Control Group ◽  
Antioxidant Compounds ◽  
Standard Diet ◽  
Nuclear Factor ◽  
Control Group Design ◽  
Nuclear Factor Kappa ◽  
Watermelon Rind ◽  
Nuclear Factor Kappa Beta

Atherogenic diet can lead to dyslipidemia condition that trigger increase Reactive Oxygen Species (ROS) and oxidative stress. The increasing of ROS can activate transcription factor Nuclear Factor Kappa Beta (NF-kβ). Activated NF-kβ induce proinflammatory cytokines that will increase the atherosclerosis progressivity. Watermelon rind had proven to have antioxidant compounds that can inhibit ROS. This study was aimed to prove the effect of watermelon rind extract and simvastatin to decrease the NF-kβ aktivation of Rattus norvegicus with atherogenic diet. This research was using Post Test Only Control Group Design. The samples were divided into five groups: (i) standard diet (K(-)), (ii) atherogenic diet (K(+)), (iii) atherogenic diet + watermelon rind extract 250 mg/kgBW/day, (iv) atherogenic diet + watermelon rind 500 mg/kgBW/day, and (v) the atherogenic diet + simvastatin 0.9 mg/kgBW/day. Atherogenic diet was given for 8 weeks. NF-kβ were measured by immunohistochemistry. Data were analyzed with ANOVA and post hoc test. The results showed a significant decrease mean NF-kβ activation after treatment of watermelon rind extract of 250 mg/kg/day (p =0,000), dose of 500 mg/kg/day (p = 0.000), and simvastatin dose of 0.9 mg/kg/day could reduced NF-kβ significantly (p = 0,000) compared with atherogenic diet group. It concluded that the watermelon rind extract dose 500 mg/kgBW/day was equivalent to simvastatin 0,9 mg/kgBW/day in lowering the NF-kβ activation rats with atherogenic diet.

scholarly journals The Role of Bamboo Shoot Gigantochloa apus Extract in Decreasing MDA and Increasing IL-10 at The Atherosclerosis

2019 ◽  
Vol 3 (1) ◽  
pp. 23-27
Author(s):  
Edy Soesanto ◽  
Edi Dharmana ◽  
Soeharyo Hadisaputro ◽  
Siti Fatimah Muis
Keyword(s):  
Atherogenic Diet ◽  
Control Group ◽  
Antioxidant Compounds ◽  
Bamboo Shoot ◽  
Control Group Design ◽  
Treatment Groups ◽  
New Zealand White Rabbits ◽  
Post Test ◽  
Free Radical Formation

Introduction: Bamboo shoot Gigantochloa apus extract has antioxidant compounds that act as lipid peroxidation inhibitors and reduce free radical formation so that it can be used as an anti-inflammatory and anti-oxidative stress in the atherosclerosis. Aim: Knowing the effect of bamboo shoot Gigantochloa apus extract in reducing MDA levels and IL-10 increasing levels in rabbits given atherogenic diet. Methods: This experiment used randomized pre-test and post-test with control group design, in 24 New Zealand White rabbits divided into 4 groups randomly. MDA and IL-10 levels were examined by the ELISA method. Results and conclusion: Bamboo shoot Gigantochloa apus extract can reduce MDA levels and increase IL-10 levels significantly in accordance with increasing doses. The increase of MDA levels in the control group with all treatment groups was different (p = 0.0001), and between the treatment groups and other treatment groups there were also differences (p

scholarly journals Borate reduces experimental supra-celiac aortic clamping-induced oxidative stress in lung and kidney, but fails to prevent organ damage

2021 ◽  
Vol 29 (3) ◽  
pp. 320-329
Author(s):  
Tünay Kurtoğlu ◽  
Selim Durmaz ◽  
Ömer Faruk Rahman ◽  
Nesibe Kahraman Çetin ◽  
Mustafa Yılmaz ◽  
...  
Keyword(s):  
Dimethyl Sulfoxide ◽  
Ischemia Reperfusion ◽  
Interleukin 1 ◽  
Control Group ◽  
Nuclear Factor ◽  
Necrosis Factor Alpha ◽  
Aortic Clamping ◽  
Nuclear Factor Kappa ◽  
Histological Damage ◽  
Nuclear Factor Kappa Beta

Background: This study aims to investigate the effects of 2-aminoethoxydiphenyl borate (2-APB) on aortic clamping-induced lung and kidney tissue oxidation, tissue inflammation, and histological damage in a rat model. Methods: A total of 28 adult female Wistar albino rats were randomly allocated to four equal groups: Control group, ischemia-reperfusion group, dimethyl sulfoxide group, and 2-APB group. Animals in the control group underwent median laparotomy. In the remaining groups, supra-celiac aorta was clamped for 45 min and, then, reperfusion was constituted for 60 min. The 2-APB (2 mg/kg) was administered before clamping. The remaining groups received saline (ischemia-reperfusion group) or dimethyl sulfoxide (dimethyl sulfoxide group). Kidney and lung tissue samples were harvested at the end of reperfusion. Results: Aortic occlusion caused increased tissue total oxidant status and reduced total antioxidant status and glutathione levels in the ischemia-reperfusion and dimethyl sulfoxide groups. Tissue interleukin-1 beta and tumor necrosis factor-alpha levels, nuclear factor kappa beta activation, and histological damage severity scores were also higher in these groups. The 2-APB treatment eliminated the increase in total oxidant status and the decrease in total antioxidant status and glutathione levels. It also caused a decrease in the interleukin-1 beta levels, although it did not significantly alter the tumor necrosis factor-alpha levels, nuclear factor kappa beta immunoreactivity, and histological damage scores. Conclusion: Borate exerted a beneficial antioxidant effect as evidenced by reduced oxidative stress; however, it did not inhibit nuclear factor kappa beta activation and prevent histological damage in supra-celiac aortic clamping-induced kidney and lung injury in rats.

scholarly journals Pengaruh Pemberian Sari Buah Markisa Ungu (Passiflora edulis Var. Edulis sims) terhadap Ketebalan Dinding Aorta Tikus (Rattus norvegicus ) Strain Wistar yang Diberi Diet Aterogenik

2020 ◽  
Vol 3 (2) ◽  
pp. 159
Author(s):  
Hanif Alamudin Manshur ◽  
Hiya Alfi Rahmah
Keyword(s):  
Rattus Norvegicus ◽  
Aortic Wall ◽  
Optical Microscope ◽  
Atherogenic Diet ◽  
Passion Fruit ◽  
Control Group ◽  
Control Group Design ◽  
Passiflora Edulis ◽  
Wistar Strain ◽  
Purple Passion Fruit

Coronary heart disease which still become a health problem in the world and included the main causes of death is a clinical manifestation of atherosclerosis. Atherosclerosis is an accumulation of cholesterol crystals on arteries wall. Purple passion fruit concentrate (Passiflora edulis var. edulis Sims) contains β-carotene, vitamine C and flavonoids compounds. Those are antioxidants that may inhibit atherosclerosis through hypolipidemic and antioxidative effects. The purpose of this study was to determine the effect of purple passion fruit concentrate (Passiflora edulis var.edulis Sims) to the thickness of aortic wall of rats (Rattus norvegicus wistar strain) that had been given atherogenic diet. This research was experimental, with posttest only control group design. The study was conducted for 60 days using 30 male wistar rats which was divided randomly into 5 groups: K (-) (normal diet), K (+) (atherogenic diet), P1 (atherogenic diet + passion fruit concentrate 17,5 ml/kgBW ), P2 (atherogenic diet + passion fruit concentrate 21,87 ml/kgBW ), and P3 (atherogenic diet + passion fruit juice 26,25 ml /kgBW). Dependent variable was the thickness of the aortic wall, which was measured by using a optical microscope completed with ocular micrometer at a magnification of 400 times. Analysis of data using One Way ANOVA followed by Post Hoc Tuckey. The results showed that administration of the purple passion fruit concentrate (Passiflora edulis var. Edulis Sims) could reduce the thickness of aortic wall of rats (Rattus norvegicus wistar strain) which were exposed to atherogenic diet with an optimal dose was 17.5 ml / kgBW/day (p <0.001). Suggestions of this study was to test the effectiveness of the purple passion fruit concentrate when applied to humans.

scholarly journals COMPARISON OF THE EFFECTIVENESS OF PHYSICAL TRAINING AND EXTRACT OF SOURSOP LEAF TO HISTOPATHOLOGY OF ABDOMINAL AORTA FOAM CELLS IN HIPERCOLESTEROLEMIA-DIABETES RAT

2020 ◽  
Vol 3 (1) ◽  
pp. 37-50
Author(s):  
Tika Hamidah Apriliana Nai ◽  
Retno Yulianti ◽  
Wahyunia Likhayati Septiana ◽  
Yuni Setyaningsih
Keyword(s):  
Diabetes Mellitus ◽  
Physical Training ◽  
Public Health Problem ◽  
Foam Cells ◽  
Atherogenic Diet ◽  
Control Group ◽  
Standard Diet ◽  
Control Group Design ◽  
Significant Difference ◽  
Post Test

Background: Diabetes mellitus has become a public health problem globally. Type 2 diabetes mellitus, caused by lifestyle and high lipid diet, is the most common form. Hypercholesterolemia-diabetes is able to create endothelial injury that results in formation of atherosclerosis, starting with formation of foam cells. Objective: This research aimed to see the effectiveness of physical training and soursop (Annona muricata) leaves extract in lowering the amount of foam cells.Methods: This research was an experimental study which used the post test-only control group design with 35 Wistar strain rats which were randomly divided into 7 groups, each consisting of 5 rats. K1 was given standard diet, K2 was given atherogenic diet and alloxan, while K3, K4, K5, K6, and K7 were given atherogenic diet, alloxan, and different treatments based on designed group. K3 was given simvastatin, K4 was given metformin, K5 was given physical training, K6 was given soursop leaves extract (SLE), and K7 was given both physical training and SLE. The rats were euthanized after three weeks and aorta was taken to be made into histology slides for foam cells observation.Results: The result of Kruskal-Wallis and Mann-Whitney Post-Hoc Test showed significant difference (p<0,05) between K2 and K5, K6, K7 while it no significant difference (p>0,05) between K3, K4 and K5, K6, K7.Conclusion: Physical training, SLE, and the combination of both have a role in lowering formation of foam cells in atherosclerosis.

scholarly journals Pengaruh pemberian ekstrak jamur merang (volvariella volvacea) terhadap kadar kolesterol total, enzim lppla2 dan mda darah

2016 ◽  
Vol 4 (1) ◽  
pp. 48-54
Author(s):  
Amelia Eka Damayanty ◽  
Lisyani B. Suromo ◽  
RA Kisdjamiatun
Keyword(s):  
Total Cholesterol ◽  
Test Method ◽  
Control Group ◽  
Antioxidant Compounds ◽  
Standard Diet ◽  
Control Group Design ◽  
Colorimetric Test ◽  
Straw Mushroom ◽  
Post Test ◽  
Blood Total Cholesterol

Background: Consumption of high-fat diet (HFD) is increasing blood total cholesterol, LpPLA2 enzyme and MDA level which is a cause of atherosclerosis that should be controlled by antioxidants. Paddy straw mushroom (Volvariella v.) extract has an antioxidant compounds. This research proves the effect of Volvariella v. extract on blood total cholesterol (TC), LpPLA2 enzyme and MDA level, and proves correlation between TC with LpPLA2 enzyme and MDA level.Methods: A randomized post test only control group design to twenty four of 8-10 weeks old  male Rattus novergicus Wistar strain with 180-200 gram were divided into four group; K1 with standard diet, K2 with HFD, each X1 and X2 with HFD+Volvariella v. extract 500 mg/kg.bwt/day and 1000 mg/kg.bwt/day. All treatments for 56 days. TC levels assesed by enzymatic colorimetric test method, LpPLA2 enzyme with ELISA method and MDA with TBARs method. Data were analyzed by one way Anova, LSD post hoc and Pearson’s correlation.Results: TC mean of K1 (91,28±1,55 mg/dL), K2 (181,96±4,19 mg/dL), X1 (122,94±2,75 mg/dL) and X2 (105,86±2,25 mg/dL); p=0,001. Enzyme LpPLA2 mean of K1 (2,65±3,19 ng/mL), K2 (1,29±1,35 ng/mL), X1 (3,92±2,18 ng/mL),  X2 (2,05±3,29 ng/mL); p=0,385 and blood MDA mean of K1 (1,17±0,12 nmol/mL), K2 (5,75±0,21 nmol/mL), X1 (2,96±0,91 nmol/mL) and X2 (1,77±0,92 nmol/mL); p=0,001. Correlation between TC and LpPLA2 enzyme was shown by X1 group (p=0,042; r = - 0,827).Conclusion:Volvariella v. extract decreased blood total cholesterol and MDA level significantly, did not significant to LpPLA2 enzyme. There wss a correlation between TC and LpPLA2 enzyme but was not to TC and blood MDA. 

scholarly journals The Effect of Mesenchymal Stem Cell Wharton’s Jelly on Nuclear Factor Kappa Beta and Interleukin-10 Levels in Osteoarthritis Rat Model

2020 ◽  
Vol 8 (B) ◽  
pp. 350-357
Author(s):  
Vivi Sofia ◽  
Moch Saiful Bachri ◽  
Endrinaldi Endrinaldi
Keyword(s):  
Stem Cell ◽  
Mesenchymal Stem Cell ◽  
Wharton’S Jelly ◽  
Joint Disease ◽  
Control Group ◽  
Nuclear Factor ◽  
Wharton's Jelly ◽  
Knee Oa ◽  
Nuclear Factor Kappa ◽  
Nuclear Factor Kappa Beta

BACKGROUND: Osteoarthritis (OA) is a degenerative joint disease in one or more joints characterized by changes in pathological structures such as cartilage, hypertrophy, and remodeling of the subchondral bone and secondary inflammation of the synovium membrane, causing changes in joint components such as cells, matrices, and molecular production. At the molecular level, an imbalance between catabolic and anabolic activities in joint cartilage results in OA. Nuclear factor kappa beta (NFκβ) is a cytokine that plays an important role in the signaling pathway of the pathogenesis of OA in causing an inflammatory reaction, whereas interleukin (IL)-10 is an anti-inflammatory cytokine that is involved in the pathogenesis of OA. AIM: This study aims to prove the influence before and after administration mesenchymal stem cells from Wharton’s jelly on the serum NFκβ and IL-10 levels in OA rat models. MATERIALS AND METHODS: This research is an experimental study with the design of post-test-only control group design. The sample consisted of 16 OA rats as a control group and 16 OA rats treated with MSC-WJ as a treatment group. OA induction is done by injection of monosodium iodoacetate (MIA) into the intra-articular right knee. Giving MSC-WJ is done in the 3rd week after MIA induction. The serum NFκβ and IL-10 levels were measured after 3 weeks treated with MSC-WJ using the ELISA method. The statistical test used is an independent t-test. p < 0.05 was said to be statistically significant. RESULTS: From the research results obtained, serum levels of knee OA of rat knee OA treated with mesenchymal stem cell Wharton jelly are lower than serum NFκβ levels of knee OA of the rat that is not treated, but the difference in levels of NFκβ is not significant (p > 0.05). The serum IL-10 level of rat OA of knee treated with mesenchymal stem cell Wharton jelly was higher than the serum IL-10 level of rat OA of the knee that was not treated, difference in levels of IL-10, is significant (p < 0.05). CONCLUSION: This study concluded that MSC-WJ significantly decreased the serum NFκβ levels of OA rats and not significantly increased the serum IL-10 levels of OA rats.

Pengaruh Pemberian Ekstrak Rumput Laut Merah (Kappaphycus alvarezii) terhadap Kadar LDL Pada Tikus Putih (Rattus Norvegicus) Jantan Galur Wistar Yang Diberi Diet Tinggi Lemak

2018 ◽  
Vol 15 (2) ◽  
pp. 112
Author(s):  
MENTARI AMENDA SAPUTRI ◽  
HERIN SETIANINGSIH
Keyword(s):  
Rattus Norvegicus ◽  
Kappaphycus Alvarezii ◽  
Control Group ◽  
Control Group Design ◽  
Group Design ◽  
Post Test ◽  
One Way Anova ◽  
Test Control

<p class="Default">Penyakit kardiovaskular merupakan penyebab kematian nomor satu di dunia. Gaya hidup masyarakat terutama dalam mengkonsumsi diet yang tidak sehat dapat meningkatkan kadar LDL yang dapat menyebabkan  penyakit kardiovaskular. Rumput laut merah (<em>Kappaphycus alvarezii</em>)<em> </em>yang banyak dibudidayakan di Indonesia mengandung flavonoid dan triterpenoid yang diduga dapat menurunkan kadar LDL. Penelitian ini bertujuan untuk mengetahui pengaruh pemberian ekstrak rumput laut merah (<em>Kappaphycus alvarezii</em>) <em> </em>terhadap kadar LDL pada tikus putih (<em>Rattus norvegicus</em>) jantan galur Wistar yang diberi diet tinggi lemak. Penelitian ini adalah penelitian eksperimental murni laboratorik dengan rancangan penelitian <em>Post Test Control Group Design. </em>Sampel yang digunakan adalah 24 ekor tikus putih (<em>Rattus norvegicus</em>) jantan galur Wistar yang dibagi ke dalam tiga kelompok: kelompok yang diberi diet standar selama 28 hari (K1), kelompok yang diberi diet tinggi lemak selama 28 hari (K2), dan kelompok yang diberi diet tinggi lemak selama 28 hari dan pada hari ke-15 sampai hari ke-28 diberi ekstrak rumput laut merah (<em>Kappaphycus alvarezii</em>) dengan dosis 140mg/200grBB/hari (K3). Hasil analisis statistik <em>One Way Anova </em>menunjukkan bahwa terdapat perbedaan kadar LDL yang signifikan antara ketiga kelompok pada penelitian ini (p&lt;0,001). Kadar LDL pada K2 (=16,00±3,29) meningkat secara bermakna dibandingkan dengan K1 (=10,62±1,77). Sedangkan kadar LDL pada K3 (=6,88±2,42) menurun secara bermakna dibandingkan dengan K2. Dari hasil penelitian ini dapat disimpulkan bahwa ekstrak rumput laut merah (<em>Kappaphycus alvarezii</em>) berpengaruh terhadap kadar LDL darah pada tikus putih (<em>Rattus norvegicus</em>) jantan galur Wistar yang diberi diet tinggi lemak.</p><p><strong>Kata kunci</strong> : diet tinggi lemak, LDL, <em>Kappaphycus alvarezii</em></p>

PERBEDAAN PROFIL LIPID TIKUS RATTUS NORVEGICUS PADA PEMBERIAN DIET KETOGENIK LEMAK JENUH DAN LEMAK TIDAK JENUH SELAMA EMPAT MINGGU

2021 ◽  
Vol 4 (2) ◽  
pp. 178-188
Author(s):  
Dewa Ayu Liona Dewi ◽  
Ari Christy Muliono ◽  
Gladdy Lysias Waworuntu ◽  
Evelyn Ongkodjojo
Keyword(s):  
Rattus Norvegicus ◽  
Control Group ◽  
Control Group Design ◽  
Group Design

Latar Belakang. Diet ketogenik merupakan diet populer untuk menurunkan berat badan dengan efek terhadap profil lipid masih inkonsisten. Tujuan Penelitian. Menganalisis perbedaan profil lipid tikus Rattus norvegicus pada pemberian pakan ketogenik dengan komposisi  lemak 80% (jenuh dan tidak jenuh), protein 15% dan karbohidrat 5%  selama 4 minggu. Metodologi. Desain penelitian true experimental dengan posttest only control group design. Tikus jantan dengan berat badan 125-150 g  dibagi secara random dalam 3 kelompok (P0, P1 dan P2) menerima diet 25-30 kkal/hari sesuai dengan kebutuhan energi harian tikus. P0 kelompok kontrol menerima pakan tikus biasa. P1 menerima diet ketogenik lemak jenuh dari margarin. P2 menerima diet ketogenik lemak tak jenuh dari minyak zaitun ekstra virgin. Kolesterol total, LDL, HDL dan trigliserida diperiksa pada minggu ke 2 dan 4. Data dianalisis dengan Uji t Independen dan Mann Whitney (p<0,05). Hasil. Pada P1 terdapat perbedaan kadar HDL yang signifikan (38 menjadi 22,40 mg/dL, p=0,005) namun perbedaan kadar kolesterol total, LDL dan kadar trigliserida tidak signifikan. Pada P2 didapatkan perbedaan yang signifikan pada kadar kolesterol total (93,20 menjadi 67,80 mg/dL, p=0,042), HDL (29,40 menjadi 22,20 mg/dL, p=0,004), LDL (25,60 menjadi 13,80 mg/dL, p=0,009), dan trigliserida (100,80 menjadi 51,40 mg/dL, p=0,016). Pada P0 tidak ada perbedaan yang signifikan. Kesimpulan. Diet ketogenik dengan lemak tidak jenuh selama 4 minggu  menunjukkan efek yang menguntungkan dalam menurunkan kadar kolesterol total, LDL dan trigliserida pada tikus Rattus norvegicus. kata kunci: diet ketogenik, lemak jenuh, lemak tidak jenuh, kolesterol total, HDL, LDL, trigliserida

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