STUDY THE HARMFUL EFFECTS OF SODIUM METABISULFITE ON LIVER, TESTEs, AND LIPID PEROXIDATION IN MALE MICE, (MUS MUSCULUS)
The present study was designed to evaluate the toxic effect of Sodium metabisulfite (SMB) on testes and liver of 8 week- old mouse, which were grouped as control (C) and dose group, SM (500 mg/kg B.W SMB). The Experimental group was injected 0.1ml SM (500 mg/g B.W + 10 ml distilled water) for twenty-one days, while a control group was given 0.1 ml water for the same durations. Recovered testes were subjected to morphological, morphometric, and histological analysis. A Hormone evaluation was also conducted. Methods Dose group SM (500 mg/kg B.W SMB) showed morphological defects like wrinkles on the surface, shrinkage, small size, whereas the testes of control group appeared normal just like control (C). Morphometric analysis of dose group SM (500 mg/g B.W SMB) showed significant decrease in length, width and weight of testis as compared to control group. Blood analysis showed significantly levels of testosterone and glutathione reductions in dose group SM (500 mg/g B.W SMB) as compared to the control group. While the level of Melanodialdehyde (MDA) increased significantly in dose group SM (500 mg/g B.W SMB) as compare to the control group. Histological defects were observed in testes of dose group SM (500 mg/g B.W SMB) like deshaped somniferous tubule, degenerated spermatids, vacuolization, rupturing of germinal epithelium, absence of sperms in the lumen, degenerated peritubular myoid cells, hyalinization, cluster of degenerated spermatozoa in lumen and absence of leydig cells. Histology of the liver showed various defects like mitotic cells, vacuolization, degeneration of hepatic cells and cell shrinkage, broad suicidal spacing, absence of bile canaliculus cells, regenerated kuppfer cell, and interrupted sheet of hepatocytes. Conclusion - The harmful impact of sodium metabisulfite on the liver and reproductive system of male mice. A Sharp decrease in the level of testosterone was formed to be a key factor for studied deformation in morphology, morphometry, and histol