scholarly journals Lack of Offspring Nrf2 Does Not Exacerbate the Detrimental Metabolic Outcomes Caused by In Utero PCB126 Exposure

2021 ◽  
Vol 12 ◽  
Author(s):  
Brittany B. Rice ◽  
Sara Y. Ngo Tenlep ◽  
Obadah Tolaymat ◽  
Attaas T. Alvi ◽  
Fallon R. Slone ◽  
...  
Keyword(s):  
Body Composition ◽  
Body Weight ◽  
Glucose Tolerance ◽  
In Utero ◽  
Whole Body ◽  
Glucose Challenge ◽  
Nrf2 Expression ◽  
Average Body ◽  
Average Body Weight ◽  
Pcb Exposure

Human environmental exposures to toxicants, such as polychlorinated biphenyls (PCBs), increase oxidative stress and disease susceptibility. Such exposures during pregnancy and/or nursing have been demonstrated to adversely affect offspring health outcomes. Nuclear factor erythroid-2-related factor 2 (Nrf2) regulates the antioxidant response and is involved in the detoxification of coplanar PCBs, like PCB126. The purpose of this study was to investigate glucose tolerance and body composition in PCB-exposed offspring expressing or lacking Nrf2. We hypothesized that offspring lacking Nrf2 expression would be more susceptible to the long-term health detriments associated with perinatal PCB exposure. During gestation, whole-body Nrf2 heterozygous (Het) and whole-body Nrf2 knockout (KO) mice were exposed to vehicle or PCB126. Shortly after birth, litters were cross-fostered to unexposed dams to prevent PCB exposure during nursing. Offspring were weaned, and their body weight, body composition, and glucose tolerance were recorded. At two months of age, PCB exposure resulted in a significant reduction in the average body weight of offspring born to Nrf2 Het dams (p < 0.001) that primarily arose from the decrease in average lean body mass in offspring (p < 0.001). There were no differences in average body weight of PCB-exposed offspring born to Nrf2 KO dams (p > 0.05), and this was because offspring of Nrf2 KO dams exposed to PCB126 during pregnancy experienced a significant elevation in fat mass (p = 0.002) that offset the significant reduction in average lean mass (p < 0.001). Regardless, the lack of Nrf2 expression in the offspring themselves did not enhance the differences observed. After an oral glucose challenge, PCB-exposed offspring exhibited significant impairments in glucose disposal and uptake (p < 0.05). Offspring born to Nrf2 Het dams exhibited these impairments at 30 min and 120 min, while offspring born to Nrf2 KO dams exhibited these impairments at zero, 15, 30, 60 and 120 min after the glucose challenge. Again, the interactions between offspring genotype and PCB exposure were not significant. These findings were largely consistent as the offspring reached four months of age and demonstrate that the lack of offspring Nrf2 expression does not worsen the metabolic derangements caused by in utero PCB exposure as we expected. Future directions will focus on understanding how the observed maternal Nrf2 genotypic differences can influence offspring metabolic responses to in utero PCB exposure.

CASTRATION-INDUCED OBESITY IN MICE.

1966 ◽  
Vol 53 (4) ◽  
pp. 571-583 ◽  
Author(s):  
Franz X. Hausberger ◽  
Brigitte C. Hausberger
Keyword(s):  
Body Composition ◽  
Body Weight ◽  
Adrenal Cortex ◽  
Body Fat ◽  
Obese Mice ◽  
Commercial Diet ◽  
Obesity In Mice ◽  
Islet Changes ◽  
Average Body ◽  
Average Body Weight

ABSTRACT Body composition and histology of the adrenal cortex and the islets of Langerhans were compared in yellow-obese mice, their intact non-obese littermates and their castrated, originally non-obese littermates. In our colony, yellow females were heavier and fatter than males. Obesity developed in yellow mice of both sexes which were fed a commercial diet containing 12% fat. The extractable body fat of the yellow animals was more than twice as high as that of controls. The fat content of gonadectomized animals was significantly higher than that of the yellow obese mice although their average body weight was slightly less. The adrenal cortex of the castrates showed subcapsular hypertrophy, and the islets of the pancreas were enlarged due to hypertrophy and hyperplasia of the beta cells. The literature concerning obesity and adrenal cortical and islet changes, after castration, has been reviewed and the thesis has been discussed that cortical changes after castration stimulate accelerated release of insulin and obesity.

Effects of chronic spinal cord injury on body weight and body composition in rats fed a standard chow diet

2007 ◽  
Vol 293 (3) ◽  
pp. R1102-R1109 ◽  
Author(s):  
Stefany D. Primeaux ◽  
Melissa Tong ◽  
Gregory M. Holmes
Keyword(s):  
Spinal Cord ◽  
Spinal Cord Injury ◽  
Body Composition ◽  
Body Weight ◽  
Glucose Tolerance ◽  
Fat Mass ◽  
Female Rats ◽  
Whole Body ◽  
Interscapular Brown Adipose Tissue ◽  
Cord Injury

The inability to maintain body weight within prescribed ranges occurs in a significant portion of the human spinal cord injury (SCI) population. Using a rodent model of long-term high thoracic (spinal level T3) spinal cord transection (TX), we aimed to identify derangements in body weight, body composition, plasma insulin, glucose tolerance, and metabolic function, as measured by uncoupling protein 1 (UCP1) expression in interscapular brown adipose tissue (IBAT). Sixteen weeks after SCI, body weights of injured female rats stabilized and were significantly lower than surgical control animals. At the same time point, SCI rats had a significantly lower whole body fat:lean tissue mass ratio than controls, as measured indirectly by NMR. Despite lower body weight and fat mass, the cumulative consumption of standard laboratory chow (4.0 kcal/g) and mean energy intake (kcal·day−1·100 g body wt−1) of chronic SCI rats was significantly more than controls. Glucose tolerance tests indicated a significant enhancement in glucose handling in 16-wk SCI rats, which were coupled with lower serum insulin levels. The post mortem weight of gonadal and retroperitoneal fat pads was significantly reduced after SCI and IBAT displayed significantly lower real-time PCR expression of UCP1 mRNA. The reduced fat mass and IBAT UCP1 mRNA expression are contraindicative of the cumulative caloric intake by the SCI rats. The prolonged postinjury loss of body weight, including fat mass, is not due to hypophagia but possibly to permanent changes in gastrointestinal transit and absorption, as well as whole body homeostatic mechanisms.

Effect of supplementation with select human milk oligosaccharides on artificially reared newborn rats

2021 ◽  
pp. 1-26
Author(s):  
W. Wang ◽  
C. Mu ◽  
N.A. Cho ◽  
E.W. Noye Tuplin ◽  
D.E. Lowry ◽  
...  
Keyword(s):  
Body Composition ◽  
Body Weight ◽  
Glucose Tolerance ◽  
Human Milk ◽  
Fecal Microbiota ◽  
Organ Weight ◽  
Whole Body ◽  
Human Milk Oligosaccharides ◽  
Milk Oligosaccharides ◽  
Fecal Samples

Abstract Early life nutrition fundamentally influences neonatal development and health. Human milk oligosaccharides (HMOs) are key components of breast milk, but not standard infant formula that support establishment of the newborn gut microbiota. Using an artificial rearing system, our objective was to test the effect of two HMOs on whole body and organ growth, adiposity, glucose tolerance, and fecal microbiota in young rat pups. From postnatal day 4 to 21, Sprague Dawley rats were randomized to receive one of: 1) CTR (rat milk substitute); 2) 2’FL (CTR +1.2 g/L 2’-fucosyllactose); 3) 3’SL (CTR+1.2 g/L 3’-sialyllactose); 4) 2’FL+3’SL (CTR+0.6 g/L 2’-FL+0.6 g/L 3’-SL). Body weight, bowel movements and food intake were monitored daily, fecal samples collected each week, and oral glucose tolerance, body composition, and organ weight measured at weaning. No significant differences were observed between groups in growth performance, body composition, organ weight and abundance of dominant fecal microbes. A decreased relative abundance of genus Proteus in week1 fecal samples and Terrisporobacter in week3 fecal samples (P<0.05) was suggestive of a potential pathogen inhibitory effect of 3’SL. Longitudinal changes in the fecal microbiota of artificially reared suckling rats were primarily governed by age (P =0.001) and not affected by the presence of 2’-FL and/or 3’-SL in rat milk substitutes (P =0.479). Considering the known protective effects of HMOs, further investigation of supplementation with these and other HMOs in models of premature birth, extremely low body weight, or malnutrition may show more pronounced outcomes.

Finishing steers on sorghum grain and sorghum silage. Effects of grain-to-roughage ratio, urea supplementation and hexoestrol implantation on rate of body-weight gain, feed efficiency and carcass compositions

1966 ◽  
Vol 67 (2) ◽  
pp. 191-197 ◽  
Author(s):  
J. G. Morris
Keyword(s):  
Body Composition ◽  
Body Weight ◽  
Weight Gain ◽  
Feed Efficiency ◽  
Body Weight Gain ◽  
Finishing Steers ◽  
Sorghum Silage ◽  
Sorghum Grain ◽  
Average Body ◽  
Average Body Weight

1. A study of the suitability of rations of predominately sorghum grain for the intensive finishing of steers is reported. Six groups, each of ten steers, 18 months old and of an average body-weight of 544 lb., were fed, according to a set schedule, increasing quantities of grain in the ration until two groups were each consuming rations containing 80, 90 or 100% grain. One of the two groups, fed each grain-to-roughage ratio, received 60 g. urea per head daily. Individual steers were slaughtered when they attained 900 lb. body-weight. A seventh group of ten comparable steers was slaughtered at the commencement of feeding in order to measure changes in body composition due to the nutritional treatments.

THE EFFECT OF (Morinda citrifolia L.) MEAL IN DIET ON PERFORMANCE OF BROILER

2012 ◽  
Vol 2 (1) ◽  
pp. 21-27
Author(s):  
Yosi Fenita
Keyword(s):  
Body Weight ◽  
Body Weight Gain ◽  
Morinda Citrifolia ◽  
Feed Consumption ◽  
Feed Conversion ◽  
Randomized Design ◽  
Different Levels ◽  
Effect Of Feeding ◽  
Average Body ◽  
Average Body Weight

The objective of the research was to evaluate to effect of feeding mengkudu on performances of broilers. The research design used was completely randomized design. One hundred broilers were distributed into five treatments. The treatments were different levels of mengkudu meal (0, 0.75%, 1.5%, 2.25 % and 3%). The observed measured were feed consumption, average body weight (gain) and feed conversion. Results showed that feeding mengkudu (Morinda Citrifolia L.)  no effect significant (P>0.05) on feed consumption, average body weight and feed conversion.  In conclusion, feeding mengkudu meal up to 3% (in diet) does not negatively affect feed consumption, average body weight, and feed conversion.

scholarly journals The Effects of Feed Color on Broiler Performance between Day 1 and 21

Animals ◽  
2021 ◽  
Vol 11 (6) ◽  
pp. 1511
Author(s):  
Joseph P. Gulizia ◽  
Kevin M. Downs
Keyword(s):  
Body Weight ◽  
Weight Gain ◽  
Treatment Effects ◽  
Body Weight Gain ◽  
Experimental Period ◽  
Feed Consumption ◽  
Feed Conversion ◽  
Broiler Performance ◽  
Average Body ◽  
Average Body Weight

Two trials were conducted to determine feed color effects on broiler performance. A completely randomized design was used. Trial 1 included four treatments: control (complete broiler starter diet), red, green, and blue; and Trial 2 included four treatments: control, orange, yellow, and purple. Each trial had 4 treatments with 4 replicates (60 birds/treatment) fed to 240 male Cobb 500 broilers during a 21 d grow out. Data were analyzed using the GLM procedure. In Trial 1, there were no treatment effects on average body weight, body weight gain, and feed consumption (p > 0.05). Adjusted feed conversion for control (1.23) was less than red (1.27; p = 0.001) and green (1.26; p = 0.009), with blue (1.25; p = 0.056) tending to be different during the experimental period. In Trial 2, there were no treatment effects on average body weight, feed consumption, and adjusted feed conversion during this study (p > 0.05). Body weight gain between d 1 to 14 for purple (490.78 g/bird) was more than orange (467 g/bird; p = 0.013) and yellow (461 g/bird; p= 0.004), with control (474 g/bird; p = 0.052) tending to be different. Results indicate that these feed colors had some, albeit limited, influence on broiler performance parameters.

scholarly journals Minimal impact of age and housing temperature on the metabolic phenotype of Acc2−/− mice

2015 ◽  
Vol 228 (3) ◽  
pp. 127-134 ◽  
Author(s):  
Amanda E Brandon ◽  
Ella Stuart ◽  
Simon J Leslie ◽  
Kyle L Hoehn ◽  
David E James ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Body Composition ◽  
Energy Expenditure ◽  
Glucose Tolerance ◽  
Fat Mass ◽  
Knockout Mice ◽  
Muscle Glycogen ◽  
Insulin Action ◽  
Metabolic Phenotype ◽  
Whole Body

An important regulator of fatty acid oxidation (FAO) is the allosteric inhibition of CPT-1 by malonyl-CoA produced by the enzyme acetyl-CoA carboxylase 2 (ACC2). Initial studies suggested that deletion of Acc2 (Acacb) increased fat oxidation and reduced adipose tissue mass but in an independently generated strain of Acc2 knockout mice we observed increased whole-body and skeletal muscle FAO and a compensatory increase in muscle glycogen stores without changes in glucose tolerance, energy expenditure or fat mass in young mice (12–16 weeks). The aim of the present study was to determine whether there was any effect of age or housing at thermoneutrality (29 °C; which reduces total energy expenditure) on the phenotype of Acc2 knockout mice. At 42–54 weeks of age, male WT and Acc2−/− mice had similar body weight, fat mass, muscle triglyceride content and glucose tolerance. Consistent with younger Acc2−/− mice, aged Acc2−/− mice showed increased whole-body FAO (24 h average respiratory exchange ratio=0.95±0.02 and 0.92±0.02 for WT and Acc2−/− mice respectively, P<0.05) and skeletal muscle glycogen content (+60%, P<0.05) without any detectable change in whole-body energy expenditure. Hyperinsulinaemic–euglycaemic clamp studies revealed no difference in insulin action between groups with similar glucose infusion rates and tissue glucose uptake. Housing Acc2−/− mice at 29 °C did not alter body composition, glucose tolerance or the effects of fat feeding compared with WT mice. These results confirm that manipulation of Acc2 may alter FAO in mice, but this has little impact on body composition or insulin action.

Early sex determination of Turkey by observation of differences in body weight between male and female

2021 ◽  
Vol 31 (3) ◽  
pp. 218-226
Author(s):  
S Akter ◽  
SC Das ◽  
AS Apu ◽  
T Ahmed ◽  
A Lahiry ◽  
...  
Keyword(s):  
Body Weight ◽  
Male And Female ◽  
Live Body Weight ◽  
Commercial Broiler ◽  
Percent Accuracy ◽  
Similar Body ◽  
Black Color ◽  
Average Body ◽  
Average Body Weight

The present study was conducted to determine the early sex in turkeys by observation of the differences in body weight between male and female birds. A total of 30-day old black color unsexed poults having almost similar body weight at hatching were considered for the experimentation and housed at the Poultry Farm of Bangladesh Agricultural University, Mymensingh-2202, Bangladesh. All birds were reared up to 12 weeks of age under intensive management with supplementation of commercial broiler starter and grower feeds. Birds were reared under similar management conditions. Significantly higher (p<0.01) body weight was attained in male poults (104g/bird) than the female (90g/bird) at the end of 1st week of age. Similarly, at the end of 2nd week of age higher (p<0.01) body weight attained by male poults (198.31g/bird) than the female (162.13g/bird). At the end of 3rd weeks of age male poults attained higher (p<0.01) body weight (307.23g/bird) than the female (251.33g/bird). After 4 weeks of rearing, male turkeys attained significantly higher (p<0.01) live body weight (424.46g/bird) than the female turkeys (347.87g/bird). The weekly average body weight gains of male and female birds were 94.18g/bird and 76.5g/bird, respectively. Thus, the male and female birds were successfully identified on the basis of differences in their body weight. Weekly feed intake for both the male and female birds was also increased with their age. Up to 4 weeks of age, both the male and female poults consumed same amount of feed (753.46g/bird). The FCR of male and female poults differed non-significantly in 1st, 3rd and 4th week. On the contrary, in 2nd week of age the FCR of male poults (1.60) was significantly lower (p<0.01) than female (2.11). Survivability was 100% up to 4th week of age irrespective of sex of the poults. The birds were reared up to 12 weeks of age until to confirm their sex by observation of the phenotypic appearance. Results of the phenotypic observation of male and female birds correspondence hundred percent accuracy with the results obtained in body weight based differences between male and female birds. It is therefore concluded that farmers can identify male or female poults as early as first week of age on the basis of body weight differences. Progressive Agriculture 31 (3): 218-226, 2020

scholarly journals Quantitative characteristics of the carcasses of Moxotó and Canindé goats fed diets with two different energy levels

2010 ◽  
Vol 39 (7) ◽  
pp. 1565-1570 ◽  
Author(s):  
Ana Cristina Chacon Lisboa ◽  
Dermeval Araújo Furtado ◽  
Ariosvaldo Nunes de Medeiros ◽  
Roberto Germano Costa ◽  
Rita de Cássia do Egypto Queiroga ◽  
...  
Keyword(s):  
Body Weight ◽  
Energy Level ◽  
Energy Levels ◽  
Experimental Period ◽  
Carcass Yield ◽  
Final Weight ◽  
Quantitative Characteristics ◽  
Energetic Level ◽  
Average Body ◽  
Average Body Weight

The objective of this work was to evaluate the quantitative characteristics of carcasses of Moxotó and Canindé goats finished in feedlots and fed diets of two levels of energy. It was used 40 castrated young goats, 20 from Moxotó breed and 20 from Canindé breed, each one with an average body weight of 15.2 kg. The animals were fed with diets containing 2.20 and 2.71 (Mcal/kg DM). The experimental period lasted 86 days. There was no difference among the barometric measures of Moxotó and Canindé kids. Canindé goats showed higher final weight, higher weight after fasting and higher hot and cold carcass weights when compared to the Moxotó animals. Animals that consumed diets with higher energy level had higher final weight and conformation as well as higher carcass yield. Considering the percentage values of the commercial meat cuts, no difference among treatments was observed. The diet was the factor that most influenced the non-constituent components of the carcass of Moxotó and Canindé goats. In general, animals belonging to the Canindé breed presented higher quantitative characteristics than the Moxotó breed. Supply of diets with higher energetic level (2.71 Mcal/kg DM) for goats from Moxotó and Canindé breeds, in feedlots, favors the production of carcass with higher yields and heavier commercial meat cuts.

scholarly journals INTERAKSI GENOTIPE DENGAN LINGKUNGAN, ADAPTABILITAS, DAN STABILITAS PENAMPILAN FENOTIPIK EMPAT VARIETAS UNGGUL IKAN NILA (Oreochromis niloticus)

2015 ◽  
Vol 10 (1) ◽  
pp. 1
Author(s):  
Didik Ariyanto ◽  
Nunuk Listiyowati
Keyword(s):  
Body Weight ◽  
Oreochromis Niloticus ◽  
Crude Protein ◽  
Genetic Factor ◽  
Environment Factors ◽  
Pelleted Feed ◽  
Environment Factor ◽  
Net Cages ◽  
Average Body ◽  
Average Body Weight

Penampilan fenotipik suatu organisme ditentukan oleh faktor genetik dan dipengaruhi oleh faktor lingkungan tempat organisme tersebut hidup. Dalam beberapa kasus, interaksi antara faktor genetik dengan lingkungan juga berpengaruh terhadap penampilan fenotipik yang dihasilkan. Penelitian ini bertujuan untuk mempelajari interaksi antara faktor genetik dengan lingkungan, daya adaptabilitas dan stabilitas penampilan fenotipik khususnya bobot individu pada empat varietas unggul ikan nila yang dipelihara di tiga lokasi berbeda. Ikan nila varietas GMT, merah NIFI, NIRWANA dan BEST dipelihara di tambak payau di Indramayu, kolam air tenang di Sukamandi dan karamba jaring apung di Waduk Cirata, Kabupaten Cianjur, selama empat bulan pemeliharaan. Benih ikan nila diberi pakan pelet komersial dengan kandungan protein kasar 28%-30%, sebanyak 10%; 7,5%; 5%; dan 2,5% dari biomassa ikan masing-masing pada bulan pertama, kedua, ketiga, dan keempat. Pakan diberikan dengan frekuensi dua kali setiap hari. Hasil penelitian menunjukkan bahwa bobot ikan nila secara nyata dipengaruhi oleh faktor genetik, lingkungan, serta interaksi antara kedua faktor tersebut. Selain ikan nila GMT, tiga varietas ikan nila yang diuji mempunyai adaptabilitas lingkungan relatif sempit, serta stabilitas penampilan fenotipik karakter bobot individu relatif rendah. Hal ini mengindikasikan bahwa pengembangan budidaya ikan nila GMT dapat dilakukan di semua lokasi, sedangkan ikan nila merah NIFI, NIRWANA, dan BEST akan optimal jika dilakukan di lokasi tertentu saja.Phenotypic performance was determined by genetic and environment factors and their interaction. This study was conducted to examinethe present of interaction both of genetic and environment factors, adaptability, and stability of phenotypic performance of four varieties of tilapia which maintained in three different location. GMT, red NIFI, NIRWANA, and BEST tilapia strains were reared for four months in net cages in Cirata reservoir in Cianjur, in earthen ponds in Sukamandi and in brackish water ponds in Indramayu. All fish were fed with commercial pelleted feed contained of crude protein 28%-30%, about 10%, 7.5%, 5%, and 2.5% each day in 1st, 2nd, 3rd, and 4th month, respectively. The results showed that tilapia’s phenotipic performance especially the average body weight was significantly affected by genetic factor, environment factor, and interaction both of them factors. Except the GMT, all tilapia strains used in this study have narrow phenotypic adaptability and stability, especially in average body weight. This results indicated that GMT tilapia strain can be farmed in various areas, but red NIFI, NIRWANA, and BEST tilapia strains are most likely in a specific location.

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