scholarly journals Huperzia serrata Extract ‘NSP01’ With Neuroprotective Effects-Potential Synergies of Huperzine A and Polyphenols

2021 ◽  
Vol 12 ◽  
Author(s):  
N. Callizot ◽  
ML Campanari ◽  
L Rouvière ◽  
G Jacquemot ◽  
A. Henriques ◽  
...  
Keyword(s):  
Memory Deficits ◽  
Huperzine A ◽  
Neuroprotective Activity ◽  
Reversible Inhibitor ◽  
Step Method ◽  
Neuroprotective Effects ◽  
Huperzia Serrata ◽  
Assisted Extraction ◽  
One Step ◽  
Experimental Findings

Huperzia serrata (Thunb.) Trevis is widely used in traditional asiatic medicine to treat many central disorders including, schizophrenia, cognitive dysfunction, and dementia. The major alkaloid, Huperzine A (HA), of H. serrata is a well-known competitive reversible inhibitor of acetylcholinesterase (AChE) with neuroprotective effects. Inspired by the tradition, we developed a green one-step method using microwave assisted extraction to generate an extract of H. serrata, called NSP01. This green extract conserves original neuropharmacological activity and chemical profile of traditional extract. The neuroprotective activity of NSP01 is based on a precise combination of three major constituents: HA and two phenolic acids, caffeic acid (CA) and ferulic acid (FA). We show that CA and FA potentiate HA-mediated neuroprotective activity. Importantly, the combination of HA with CA and FA does not potentiate the AChE inhibitory property of HA which is responsible for its adverse side effects. Collectively, these experimental findings demonstrated that NSP01, is a very promising plant extract for the prevention of Alzheimer’s disease and memory deficits.

scholarly journals Development of a Capillary Electrophoretic Method for the Determination of Huperzine A Concentration in Vietnamese Huperzia serrata

2021 ◽  
Vol 16 (9) ◽  
pp. 1934578X2110332
Author(s):  
Ngoc Chuong Nguyen ◽  
Dinh Vinh ◽  
Duc Tuan Nguyen ◽  
Huynh Van Thi Nguyen ◽  
Cong Luan Tran ◽  
...  
Keyword(s):  
High Efficiency ◽  
Limit Of Detection ◽  
Fused Silica ◽  
Huperzine A ◽  
Effective Length ◽  
Reversible Inhibitor ◽  
Limit Of Quantification ◽  
Electrophoretic Method ◽  
Huperzia Serrata ◽  
Relative Standard

Huperzine A, isolated from Huperzia serrata, is a potent, specific, and reversible inhibitor of acetylcholinesterase with high efficiency and low toxicity. To evaluate the presence of huperzine A in Vietnamese H serrata, a reliable capillary zone electrophoresis method was developed. The analytical conditions were established using 80 mM ammonium acetate buffer, pH 6.0, hydrodynamic injection at 50 mbar for 5 s, applied voltage of 20 kV, temperature at 25 °C, uncoated fused-silica capillary, 56 cm (50 cm effective length) × 70 µm inner diameter, and ultraviolet detection at 310 nm. The recovery rates ranged from 98.05% to 100.64%, with a relative standard deviation <2%. Good linear regression was observed in the concentration range of 1 to 500 µg/mL, with a correlation coefficient of 0.9994. The limit of detection and limit of quantification were 0.33 and 1.0 µg/mL, respectively. These results demonstrate that this method is simple, selective, and suitable for performing quality control for huperzine A derived from Vietnamese H serrata.

Neuroprotective Effects of Extracts from the Radix Curcuma aromatica on H2O2-induced Damage in PC12 Cells

2018 ◽  
Vol 21 (8) ◽  
pp. 571-582 ◽  
Author(s):  
Juxiang Liu ◽  
Lianli Zhang ◽  
Dan Liu ◽  
Baocai Li ◽  
Mi Zhang
Keyword(s):  
Oxidative Stress ◽  
Pc12 Cells ◽  
Caspase 3 ◽  
Cell Damage ◽  
Positive Control ◽  
Neuroprotective Activity ◽  
Antioxidant Enzyme Activities ◽  
Morphologic Change ◽  
Neuroprotective Effects ◽  
Etoh Extract

Aim & Objectives: Curcuminoids are characteristic constituents in Curcuma, displaying obviously neuroprotective activities against oxidative stress. As one of the Traditional Chinese Medicines from Curcuma, the radix of Curcuma aromatica is also rich in those chemicals, but its neuroprotective activity and mechanism remain unknown. The aim of the current study is to evaluate the neuroprotective effects of extracts from the radix of C. aromatica (ECAs) on H2O2-damaged PC12 cells. Material and Methods: The model of oxidative stress damage was established by treatment of 400 µM H2O2 on PC12 to induce cell damage. After the treatment of ECWs for 24 h, the cell viability, LDH, SOD, CAT and GSH were measured to evaluate the neuroprotection of ECAs on that model. The potential action mechanism was studied by measurement of level of ROS, cell apoptosis rate, mitochondrial membrane potential (MMP), morphologic change, the intracellular Ca2+ content (F340/F380) and the expressions of Bcl-2, Bax and Caspase-3. Additionally, the constituents from tested extracts were analyzed by HPLC-DAD-Q-TOF-MS method. Results: Compared with a positive control, Vitamin E, 10 µg/ml of 95% EtOH extract (HCECA) and 75% EtOH extract (MCECA) can markedly increase the rate of cell survival and enhance the antioxidant enzyme activities of SOD, CAT, increase the levels of GSH, decrease LDH release and the level of ROS, attenuate the intracellular Ca2+ overloading, reduce the cell apoptotic rate and stabilize MMP, down-regulate Bcl-2 expression, up-regulate Bax and caspase-3 expression, and improve the change of cell morphology. The chemical analysis showed that diarylheptanoids and sesquiterpenoids are the major chemicals in tested extracts and the former were richer in HCECA and MCECA than others. Conclusions: These findings indicated that the effects of HCECA and MCECA on inhibiting the cells damage induced by H2O2 in PC12 are better than other extracts from the radix of C. aromatica, and the active constituents with neuroprotective effects consisting in those two active extracts are diarylheptanoids.

Effect of Dynamic Crosslinking on Mechanical Properties of PP/EPDM Blends

1993 ◽  
Vol 58 (11) ◽  
pp. 2642-2650 ◽  
Author(s):  
Zdeněk Kruliš ◽  
Ivan Fortelný ◽  
Josef Kovář
Keyword(s):  
Mechanical Properties ◽  
Impact Strength ◽  
Shear Modulus ◽  
High Impact ◽  
Necessary Condition ◽  
Step Method ◽  
Melt Mixing ◽  
One Step ◽  
High Impact Strength ◽  
Dynamic Curing

The effect of dynamic curing of PP/EPDM blends with sulfur and thiuram disulfide systems on their mechanical properties was studied. The results were interpreted using the knowledge of the formation of phase structure in the blends during their melt mixing. It was shown, that a sufficiently slow curing reaction is necessary if a high impact strength is to be obtained. Only in such case, a fine and homogeneous dispersion of elastomer can be formed, which is the necessary condition for high impact strength of the blend. Using an inhibitor of curing in the system and a one-step method of dynamic curing leads to an increase in impact strength of blends. From the comparison of shear modulus and impact strength values, it follows that, at the stiffness, the dynamically cured blends have higher impact strength than the uncured ones.

Bismuth particles imbedded degradable nanohydrogel prepared by one-step method for tumor dual-mode imaging and chemo-photothermal combined therapy

2019 ◽  
Vol 375 ◽  
pp. 122000 ◽  
Author(s):  
Yang Xuan ◽  
Xian-Lin Song ◽  
Xiao-Quan Yang ◽  
Ruo-Yun Zhang ◽  
Zi-Yu Song ◽  
...  
Keyword(s):  
Combined Therapy ◽  
Dual Mode ◽  
Step Method ◽  
One Step

scholarly journals Bio-Based Polyurethane Networks Derived from Liquefied Sawdust

Materials ◽  
2021 ◽  
Vol 14 (11) ◽  
pp. 3138
Author(s):  
Kamila Gosz ◽  
Agnieszka Tercjak ◽  
Adam Olszewski ◽  
Józef Haponiuk ◽  
Łukasz Piszczyk
Keyword(s):  
Biomass Conversion ◽  
Structural Features ◽  
Mechanical Analysis ◽  
Process Efficiency ◽  
Hydroxyl Number ◽  
Step Method ◽  
One Step ◽  
Liquefaction Process ◽  
The Fourier Transform ◽  
Liquefaction Temperature

The utilization of forestry waste resources in the production of polyurethane resins is a promising green alternative to the use of unsustainable resources. Liquefaction of wood-based biomass gives polyols with properties depending on the reagents used. In this article, the liquefaction of forestry wastes, including sawdust, in solvents such as glycerol and polyethylene glycol was investigated. The liquefaction process was carried out at temperatures of 120, 150, and 170 °C. The resulting bio-polyols were analyzed for process efficiency, hydroxyl number, water content, viscosity, and structural features using the Fourier transform infrared spectroscopy (FTIR). The optimum liquefaction temperature was 150 °C and the time of 6 h. Comprehensive analysis of polyol properties shows high biomass conversion and hydroxyl number in the range of 238–815 mg KOH/g. This may indicate that bio-polyols may be used as a potential substitute for petrochemical polyols. During polyurethane synthesis, materials with more than 80 wt% of bio-polyol were obtained. The materials were obtained by a one-step method by hot-pressing for 15 min at 100 °C and a pressure of 5 MPa with an NCO:OH ratio of 1:1 and 1.2:1. Dynamical-mechanical analysis (DMA) showed a high modulus of elasticity in the range of 62–839 MPa which depends on the reaction conditions.

scholarly journals In Vitro Propagation, Huperzine A Content and Antioxidant Activity of Three Genotypic Huperzia serrata

Plants ◽  
2021 ◽  
Vol 10 (6) ◽  
pp. 1112
Author(s):  
Yan Yang ◽  
Liangfang Dai ◽  
Decai Wu ◽  
Limin Dong ◽  
Yisheng Tu ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
High Performance ◽  
In Vitro Propagation ◽  
Huperzine A ◽  
Induction Medium ◽  
Naphthalene Acetic Acid ◽  
Regeneration Rate ◽  
Huperzia Serrata ◽  
Chromatography Analysis

Huperzia serrata is a traditional herb and endangered Chinese medicinal material, which has attracted much attention due to its production of Huperzine A (HupA). In vitro propagation of H. serrata is considered a new way to relieve the resource pressure of H. serrata. In this study, three different genotypic wild H. serrata were used for in vitro propagation. Then, the antioxidant activity and the content of HupA in the regenerated H. serrata were investigated. The results showed the survival rate of the explant was increased to 25.37% when using multiple sterilization processes. The best induction medium for H. serrata was the Schenk and Hildebrandt (SH) medium supplemented with 0.5 mg·L−1 Naphthalene acetic acid (NAA) and 0.1 mg·L−1 2,4-Dichlorophenoxyacetic acid (2,4-D), where the regeneration rate of the explant was to 57.04%. The best proliferation medium was the SH medium with NAA (1.0 mg·L−1), as the biomass of in vitro tissue increased 164.17 ± 0.41 times. High-performance liquid chromatography analysis showed that the in vitro culture of three genotypes could produce HupA and the content of HupA was 53.90–87.17 µg·g−1. The antioxidant experiment showed that the methanol extract of in vitro H. serrata had higher antioxidant activity than that of wild H. serrata. This study provides a reliable in vitro H. serrata culture protocol and laid an important foundation for the antioxidant capacity of the thallus and the content of HupA.

Discussion on Repair Method of Concrete Highway Pavement

2013 ◽  
Vol 321-324 ◽  
pp. 209-212
Author(s):  
Chun Yan Xia
Keyword(s):  
Mechanical Properties ◽  
Preparation Program ◽  
Step Method ◽  
Concrete Technology ◽  
Concrete Material ◽  
Repair Method ◽  
Ordinary Concrete ◽  
One Step ◽  
Highway Pavement

nfluences on properties of the concrete highway pavement were analyzed in this paper, and the optimal formulation materials were gotten to use in the repair of used-broken cement blocks in the experiment. Polyurethane concrete material was prepared, combing the ordinary concrete technology with one-step method of the synthesis of polyurethane hard bubble, and then its mechanical properties of the relevant parameters were measured to determine the optimal preparation program. The results show that the polyurethane concrete also has sufficiently good mechanical properties while it has the characteristic of fast patching.

scholarly journals Endophytic Fungal Community of Huperzia serrata: Diversity and Relevance to the Production of Huperzine A by the Plant Host

Molecules ◽  
2021 ◽  
Vol 26 (4) ◽  
pp. 892
Author(s):  
Lingli Cui ◽  
Hamza Armghan Noushahi ◽  
Yipeng Zhang ◽  
Jinxin Liu ◽  
Andreea Cosoveanu ◽  
...  
Keyword(s):  
Alzheimer’S Disease ◽  
Alzheimer's Disease ◽  
Fungal Community ◽  
Slow Growth ◽  
Specific Treatment ◽  
Huperzine A ◽  
Ionization Mass ◽  
Correlation Index ◽  
Plant Host ◽  
Huperzia Serrata

As the population ages globally, there seem to be more people with Alzheimer’s disease. Unfortunately, there is currently no specific treatment for the disease. At present, Huperzine A (HupA) is one of the best drugs used for the treatment of Alzheimer’s disease and has been used in clinical trials for several years in China. HupA was first separated from Huperzia serrata, a traditional medicinal herb that is used to cure fever, contusions, strains, hematuria, schizophrenia, and snakebite for several hundreds of years in China, and has been confirmed to have acetylcholinesterase inhibitory activity. With the very slow growth of H. serrata, resources are becoming too scarce to meet the need for clinical treatment. Some endophytic fungal strains that produce HupA were isolated from H. serrate in previous studies. In this article, the diversity of the endophytic fungal community within H. serrata was observed and the relevance to the production of HupA by the host plant was further analyzed. A total of 1167 strains were obtained from the leaves of H. serrata followed by the stems (1045) and roots (824). The richness as well as diversity of endophytic fungi within the leaf and stem were higher than in the root. The endophytic fungal community was similar within stems as well as in leaves at all taxonomic levels. The 11 genera (Derxomyces, Lophiostoma, Cyphellophora, Devriesia, Serendipita, Kurtzmanomyces, Mycosphaerella, Conoideocrella, Brevicellicium, Piskurozyma, and Trichomerium) were positively correlated with HupA content. The correlation index of Derxomyces with HupA contents displayed the highest value (CI = 0.92), whereas Trichomerium showed the lowest value (CI = 0.02). Through electrospray ionization mass spectrometry (ESI-MS), it was confirmed that the HS7-1 strain could produce HupA and the total alkaloid concentration was 3.7 ug/g. This study will enable us to screen and isolate the strain that can produce HupA and to figure out the correlation between endophytic fungal diversity with HupA content in different plant organs. This can provide new insights into the screening of strains that can produce HupA more effectively.

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