scholarly journals Slight Disruption in Intestinal Environment by Dextran Sodium Sulfate Reduces Egg Yolk Size Through Disfunction of Ovarian Follicle Growth

2021 ◽  
Vol 11 ◽  
Author(s):  
Takahiro Nii ◽  
Takashi Bungo ◽  
Naoki Isobe ◽  
Yukinori Yoshimura
Keyword(s):  
Gene Expression ◽  
Oral Administration ◽  
Blood Plasma ◽  
Sodium Sulfate ◽  
Egg Production ◽  
Laying Hens ◽  
Ovarian Follicle ◽  
Egg Yolk ◽  
Dextran Sodium Sulfate ◽  
Intestinal Environment

Intestinal environments such as microbiota, mucosal barrier function, and cytokine production affect egg production in laying hens. Dextran sodium sulfate (DSS) is an agent that disrupts the intestinal environment. Previously, we reported that the oral administration of dextran sodium sulfate (DSS: 0.9 g/kg BW) for 5 days caused severe intestinal inflammation in laying hens. However, the DSS concentration in the previous study was much higher to induce a milder disruption of the intestinal environment without heavy symptoms. Thus, the goal of this study was to determine the effects of a lower dose of DSS on the intestinal environment and egg production in laying hens. White Leghorn laying hens (330-day old) were oral administered with or without 0.225 g DSS/kg BW for 28 days (DSS and control group: n = 7 and 8, respectively). Weekly we collected all laid eggs and blood plasma samples. Intestinal tissues, liver, ovarian follicles, and the anterior pituitary gland were collected 1 day after the final treatment. Lower concentrations of orally administered DSS caused (1) a decrease in the ratio of villus height/crypt depth, occludin gene expressions in large intestine and cecal microbiota diversity, (2) a decrease in egg yolk weight, (3) an increase in VLDLy in blood plasma, (4), and enhanced the egg yolk precursor accumulation in the gene expression pattern in the follicular granulosa layer, (5) an increase in FSH and IL-1β gene expression in the pituitary gland, and (6) an increase in concentration of plasma lipopolysaccharide binding protein. These results suggested that the administration of the lower concentration of DSS caused a slight disruption in the intestinal environment. This disruption included poor intestinal morphology and decreased cecal microbiome diversity. The change in the intestinal environment decreases egg yolk size without decreasing the VLDLy supply from the liver. The decrease in egg yolk size is likely to be caused by the dysfunction of egg-yolk precursor uptake in ovarian follicles. In conclusion, the oral administration of a lower dose of DSS is an useful method to cause slight disruptions of intestinal environment, and the intestinal condition decreases egg yolk size through disfunction of ovarian follicle.

scholarly journals Comparative Effects of Flaxseed Sources on the Egg ALA Deposition and Hepatic Gene Expression in Hy-Line Brown Hens

Foods ◽  
2020 ◽  
Vol 9 (11) ◽  
pp. 1663
Author(s):  
Muhammad Suhaib Shahid ◽  
Tausif Raza ◽  
Yuqin Wu ◽  
Mazhar Hussain Mangi ◽  
Wei Nie ◽  
...  
Keyword(s):  
Gene Expression ◽  
Egg Production ◽  
Laying Hens ◽  
Low Density Lipoprotein ◽  
Density Lipoprotein ◽  
Egg Yolk ◽  
Lipoprotein Cholesterol ◽  
Egg Mass ◽  
Low Density ◽  
Low Density Lipoprotein Cholesterol

Healthy diets are necessary for both humans and animals, including poultry. These diets contain various nutrients for maintenance and production in laying hens. Therefore, research was undertaken to explore the efficiency of various dietary flaxseed sources on the n-3 deposition in the egg yolk and gene expression in laying hens. Five dietary groups were analyzed, i.e., (i) a corn-based diet with no flaxseed (FS) as a negative control (NC), (ii) a wheat-based diet supplemented with 10% whole FS without multi-carbohydrase enzymes (MCE) as a positive control (PC), (iii) ground FS supplemented with MCE (FS), (iv) extruded flaxseed meal was supplemented with MCE (EFM), (v) flaxseed oil supplemented with MCE (FSO). Results indicated that egg weight was highest in the NC, FS, EFM, and FSO groups as compared to PC in the 12th week. Egg mass was higher in enzyme supplemented groups as compared to the PC group, but lower than NC. In the 12th week, the HDEP (hen day egg production) was highest in the FS and EFM groups as compared to FSO, PC, and NC. The FCR (feed conversion ratio) was better in enzyme supplemented groups as compared to the PC group. Enzyme addition enhanced the egg quality as compared to PC in the 12th week. The HDL-C (high-density lipoprotein cholesterol) was increased, while LDL-C (low-density lipoprotein cholesterol), VLDL-C (very-low-density lipoprotein cholesterol), TC (total cholesterol), and TG (total triglycerides) were reduced in the enzyme supplemented groups as compared to PC and NC. The FSO deposit more n-3 PUFA and docosahexaenoic acid (DHA) in the egg yolk as compared to FS and EFM groups. The expression of ACOX1, LCPT1, FADS1, FADS2, and ELOV2 genes were upregulated, while PPAR-α was downregulated in the FSO group. The LPL mRNA expression was upregulated in the FS, EFM, and FSO groups as compared to the PC and NC groups. It was inferred that FSO with enzymes at 2.5% is cost-effective, improves the hen performances, upregulated the fatty acid metabolism and β-oxidation genes expression, and efficiently deposits optimal n-3 PUFA in the egg as per consumer’s demand.

scholarly journals 137 Dietary Supplementations of Ethanolamine and Serine Enhance Docosahexaenoic Acid Deposition in Eggs of Laying Hens

2020 ◽  
Vol 98 (Supplement_4) ◽  
pp. 107-108
Author(s):  
Andrew D Magnuson ◽  
Xingen Lei
Keyword(s):  
Gene Expression ◽  
Docosahexaenoic Acid ◽  
Laying Hens ◽  
Total Phospholipid ◽  
Choline Chloride ◽  
Basal Diet ◽  
Egg Yolk ◽  
Analysis Data ◽  
Mrna Levels ◽  
Dha Enrichment

Abstract Enrichment of docosahexaenoic-acid (DHA) into eggs of laying-hens may be limited by the availability phospholipids as a deposition sink. The present study was to determine if dietary supplementations of phospholipid-component molecules or synthesis-enhancers: choline, serine, and ethanolamine could elevate phospholipid and DHA enrichment in the eggs and tissues of hens. A total of 50-White-Leghorn-Shavers (42-wk old) were individually caged and divided into 5 groups (n = 10/group). The 5 groups of hens were fed the following diets for 3 wk: Diet-1 = a corn soybean-meal basal-diet, Diet-2 = Diet-1 + 4%-microalgae (Aurantiochytrium, Heliae, Gibert, AZ, 1.81 g-DHA/kg) + choline-chloride (26.3 g/kg diet, 60% purity, DSM-Co., Basel, Switzerland), Diet-3 = Diet-2 + 1.41% of L-serine (100% purity, Ajinomoto-Co., Inc., Kawasaki, Japan), Diet-4 = Diet-2 + 100 mg of ethanolamine/kg (99% purity, Sigma-Aldirch-Co., St Loius, MO), and Diet-5 = Diet-3 + 100 mg of ethanolamine/kg. At the end of study, eggs, liver, ovary, and adipose samples were collected from 6 hens/group for biochemical analysis. Data were analyzed by one-way ANOVA. Compared with Diet-1, Diet-2 enhanced (P < 0.05) DHA concentrations in egg yolk and liver by 213 mg/egg and 2.98 mg/g tissue, respectively, but decreased (P < 0.05) total phospholipid-concentrations of yolk and liver by 50%, and hepatic-mRNA levels of elongases-2/5 and desaturases-4/6 by 25–50%. Compared with Diet-2, Diet-5 enhanced (P < 0.05) DHA (by 20%) and phospholipid (by 40%) concentrations in the egg yolk, and upregulated (P < 0.05) lipid-metabolism genes involved in the citicoline (CDP, up-to-3-fold) and CDP-ethanolamine (up-to-2.5-fold) pathways in the liver and ovary-tissue. In comparison, Diets-3 and 4 resulted in only 3–11% higher (P < 0.05) DHA-concentrations in the liver over Diet-2. In conclusion, feeding hens a high DHA and choline diet enriched DHA in the egg yolk and down-regulated lipogenesis-gene-expression in the tissues. Supplementing the diet with extra-serine and ethanolamine further-enhanced the DHA enrichment in the egg yolk and restored the high DHA-mediated changes in the gene-expression. (Supported in part by DOE-MAGIC-grant DE-EE0007091, USDA-grant 2019-69012-29905, and Cornell-University-Hatch-grants NYC-127419/127302).

scholarly journals Production of laying hens in different rearing systems under hot weather

2017 ◽  
Vol 40 ◽  
pp. 37677
Author(s):  
Daniel Araujo Netto ◽  
Héder José D'Ávila Lima ◽  
Julia Rodrigues Alves ◽  
Bianca Corrêa de Morais ◽  
Mauricio Silva Rosa ◽  
...  
Keyword(s):  
Egg Production ◽  
Laying Hens ◽  
Production Systems ◽  
Egg Yolk ◽  
Feed Conversion ◽  
Negative Effects ◽  
External Quality ◽  
Weight Variation ◽  
Randomized Design

 The environment is very important for the performance of laying hens; thus, techniques are required to improve production systems, providing better welfare for poultry and consequent increase in the quality of the final product, the egg. This study aimed to evaluate the effects of rearing system, on the floor and in cage, on the performance and egg internal and external quality of laying hens. A total of 320 Hysex Brown laying hens, with 34-43 weeks days of age, was distributed in a completely randomized design, with two treatments, floor and cage, with 10 replicates each. The parameters evaluated were: individual feed intake, egg production per replicate, feed conversion per dozen eggs, egg weight; weight and percentage participation of shell, albumen and yolk, specific gravity, body weight variation and viability of birds. The results show that the birds raised on the floor showed best results as to egg production, besides the best internal and external quality, with greater weight of egg, yolk, albumen, shell and with lower losses of eggs. Because of the negative effects of the cage system, in general, hens presented lower results when compared to results of those raised on the floor. 

scholarly journals Extraction of Uronic Acid From Sargassum crassifolium and Its Feeding Effects On The Eggs Production and Quality of Lohmann Chicken Eggs

2020 ◽  
Vol 7 (3) ◽  
pp. 264
Author(s):  
Veybe Gresje Kereh ◽  
Ivonne Maria Untu ◽  
Marie Najoan ◽  
T. Lumi
Keyword(s):  
Drinking Water ◽  
Uronic Acid ◽  
Egg Production ◽  
Pathogenic Bacteria ◽  
Laying Hens ◽  
Brown Seaweed ◽  
Egg Yolk ◽  
Egg Mass ◽  
Chicken Eggs

ABSTRACTThe use of antibiotics in chicken feed is currently banned because it can cause resistance to pathogenic bacteria and cause residues in products. This study aimed to see the effect of drinking water containing uronic acid extracted from Sargassum crassifolium (S. crassifolium) on the physical and chemical quality of Lohmann chicken eggs. One hundred and twenty laying hens were divided into 2 groups: (1) chickens given commercial feed containing antibiotics and (2) feed without additional antibiotics. The chickens were randomly assigned to one of the 5 brown seaweed supplementation treatments in drinking water A1=0.0% S.crassifolium (control); A2=2.5% S.crassifolium; A3=5.0% S.crassifolium; A4=7.5% S.crassifolium; A5=10.0% S.crassifolium. The study used a completely randomized factorial design of 5 treatments, 2 factors, and 3 replications. Each replication consisted of 6 heads of laying hens. There were no differences between treatments on the performance of laying hens (egg production, egg weight, egg mass, feed conversion); physical quality (eggshell, egg yolk, egg white, shell thickness, egg yolk, Haugh unit, egg index) and chemistry (superoxide dismutase production, thiobarbituric acid reactive substances (TBARS) and Lohmann chicken egg cholesterol) but there was a difference in feed intake. The uronic acid extracted from S. crassifolium has not been able to increase the production and quality of Lohmann chicken eggs.Keywords: lohmann chicken, quality of eggs, Sargassum crassifolium, uronic acid

scholarly journals Effectivity of BS4 enzyme complex on the performance of laying hens fed with different ingredients

2016 ◽  
Vol 21 (1) ◽  
pp. 1
Author(s):  
Arnold P. Sinurat ◽  
Tresnawati Purwadaria ◽  
Tuti Haryati
Keyword(s):  
Rice Bran ◽  
Egg Production ◽  
Laying Hens ◽  
Palm Kernel ◽  
Egg Yolk ◽  
P Value ◽  
Enzyme Complex ◽  
Recorded Data ◽  
Egg Shell ◽  
Digestible Amino Acids

<p class="abstrak2">An experiment was carried out to evaluate the effectivity of an enzyme complex produced by Eupenicilium javanicum BS4 on the performance of laying hens fed with different feed ingredients. Three diets were formulated with similar nutrients (protein, ME, digestible amino acids, Ca and available P) value to meet the nutrient requirement of laying hens.  Diets were formulated based on maize, palm kernel cake (PKC) or rice bran.  The diets were either supplemented or not with BS4 enzyme complex and fed to Isa Brown pullet from 19 to 37 weeks old. Each diet was fed to 24 birds and the performances were recorded. Data were analysed statistically with 2 X 3 factorial design with six replicates. Results showed that supplementation of BS4 enzyme into laying hens diet did not influence body weight change, feed intake, mortalities, egg weight and egg shell thickness but significantly increased egg production (P&lt;0.05) and the FCR (P&lt;0.01) in any feedstuff tested. It also improved egg yolk colour score on diet contained 20% PKC. Laying hens fed 20% PKC produced more eggs than those fed maize or rice bran. The egg shell of laying hens fed rice bran were thicker than those fed with maize or PKC. It is concluded that BS4 enzyme complex is effective when supplemented into laying hens diet.</p>

scholarly journals Effect of Feeding Achiote Seed on Egg-Yolk Pigmentation

1969 ◽  
Vol 46 (2) ◽  
pp. 91-96
Author(s):  
F. T. Landagora
Keyword(s):  
Drinking Water ◽  
Puerto Rican ◽  
Egg Production ◽  
Laying Hens ◽  
Egg Yolk ◽  
Egg Yolks ◽  
Orange Color ◽  
Effect Of Feeding ◽  
Basal Ration

The yolk-coloring effect of "achiote" seed was investigated. Hens fed a ration supplemented with whole achiote seed laid eggs with deep-colored yolks. The red-orange color that was observed in the egg yolks of the achiote group persisted in both fresh and boiled eggs. Ground achiote seed fed to laying hens did not impart color to the egg yolks when added to the basal ration or when mixed with the drinking water given. The addition of whole achiote seed to the ration fed to laying hens not only colored the egg yolks more deeply, but also increased egg production. Since Puerto Rican consumers prefer eggs with deeper colored yolks this rinding has potential commercial significance.

scholarly journals EDIBLEEGGQUALITYWHENIODINEISINTRODUCEDINTOLAYINGHENBODY

2021 ◽  
pp. 65-70
Author(s):  
L.V. Rastopshina ◽  
Keyword(s):  
Egg Production ◽  
Laying Hens ◽  
Egg Quality ◽  
Federal District ◽  
Egg Yolk ◽  
Egg White ◽  
Egg Laying ◽  
The Body ◽  
Control Group ◽  
Trace Element Deficiency

The Altai Region along with the regions of the Siberian Federal District belongs to the zone with trace element deficiency including iodine deficiency. The research goal was to study the effect of iodine on edible egg quality of laying hens of the Rodonit cross. At the final stage of egg-laying, 4 groups of 4 laying hens were formed. No iodine was administered to the hens of the 1st control group. Starch-based iodine product was administered to the birds of the 2nd, 3rd and 4th trial groups into the lower third of the neck, under the wing and into the rump area in a dose of 1.5 mg per head. The egg production intensity in the trial groups was higher by 21.3-28.8%. Egg weight in the 1st control group was 65.6 ± 1.33g which was lower than that in the trial groups by 4.4% (2nd group); 3.7% (3rd group) and 1.5% 4th group). All eggs belonged to “select” grade. The 3rd and 4th trial groups had less egg white by 3.8 and 2.7%; the egg-yolk content increases in the eggs of the 2nd, 3rd and 4th trial groups by 1.2%, 9.4% and 2.4%, respectively, and the eggshell weight by 1.2-9.4% as com-pared to the control. The ratio of egg white and egg-yolk weight in the eggs of the trial groups was lower than in the control group by 9.38-19.29%, but the ratio of egg-yolk to egg white was by 10.4-23.9% higher. The following indices were higher in the trial groups as compared to the control: egg white index -by 1.4-17.1%; egg-yolk -by 3.2-6.4%; Haugh unit -by 7.2-11.1%; eggshell thickness -by 3.9-8.3%. The best results were obtained with the administra-tion of iodine to the hens in the region of the lower third of the neck. The enrichment of the body of laying hens of the Rodonit cross with iodine makes it possible to obtain the profitability of edible egg production at the level of 28.6-41.0%.

scholarly journals Supplementation of spirulina (Spirulina platensis) on yolk colour, egg quality and production performance of laying hens

2018 ◽  
Vol 34 (2) ◽  
pp. 71-78
Author(s):  
M Ahammed ◽  
S Sharmin ◽  
A Khatun ◽  
KMS Islam
Keyword(s):  
Spirulina Platensis ◽  
Egg Production ◽  
Laying Hens ◽  
Egg Quality ◽  
Dietary Supplementation ◽  
Egg Yolk ◽  
Production Performance ◽  
Dietary Treatments

A total of 96 Shaver 579 laying hens of 78 weeks of age were studied for the effects on egg production of dietary supplementation with spirulina (Spirulina platensis). The hens were divided into four dietary treatments with six replications of four birds in each group. Birds of three groups were supplemented with 0.1%, 0.2% and 0.3% spirulina and one group was kept as control. Yolk colour significantly improved (P<0.01) as compared to control, without affecting any other production performances. It is suggested that dietary supplementation of spirulina at 0.2 to 0.3% levels in aged laying hens enhanced egg yolk colour. Bangl. vet. 2017. Vol. 34, No. 2, 71-78

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