scholarly journals Morphometric Characteristics, Polyphenols and Ascorbic Acid Variation in Brassica oleracea L. Novel Foods: Sprouts, Microgreens and Baby Leaves

Agronomy ◽  
2020 ◽  
Vol 10 (6) ◽  
pp. 782 ◽  
Author(s):  
Maria Concetta Di Bella ◽  
Agnieszka Niklas ◽  
Stefania Toscano ◽  
Valentina Picchi ◽  
Daniela Romano ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Ascorbic Acid ◽  
Plant Growth ◽  
Growth Stage ◽  
Added Value ◽  
Growth Stages ◽  
Novel Food ◽  
Main Compound ◽  
Novel Foods ◽  
Freeze Dried

In the present study, we investigated the content and profile of polyphenols (PPH), ascorbic acid (AA), the Folin–Ciocalteu index (FCI), and antioxidant activity (1,1-diphenyl-2-picrylhydrazyl (DPPH) and peroxyl radical (ROO)) variation during three different plant growth stages (sprouts, microgreens and baby leaves) of two broccoli types, the traditional Sicilian sprouting broccoli landrace (‘Broccolo Nero’) and the broccoli standard (‘Cavolo broccolo Ramoso Calabrese’), and the standard commercial cultivar of kale (‘Cavolo Lacinato Nero di Toscana’). All biomasses collected were freeze-dried for PPH, AA, FCI, DPPH and ROO analysis. The highest polyphenol content was observed for ‘Broccolo Nero’ (BN) and ‘Cavolo Broccolo Ramoso Calabrese’ (CR), and generally sprouts showed significantly higher values compared to the microgreens and the baby leaves. The AA, FCI, DDPH and ROO significantly vary with regards to the cultivar and the plant growth stage, showing interaction between the two experimental factors analyzed. The interaction detected showed higher values for the antioxidant traits of the proposed novel food, especially for the two broccoli cultivars in the sprout growth stage in comparison to the microgreens and baby leaves. Our results suggest that the antioxidant activity is partially dependent on kaempferol and apigenin. The PPH compounds showed the highest values of kaempferol and apigenin for ‘Broccolo nero’, whereas for the other two cultivars studied, only kaempferol was the main compound represented. The data acquired are of interest for increasing the healthy traits of the novel food proposed showing the contribution offered by the neglected LRs until now underutilized and at risk of extinction. The germplasm conserved in several world genebanks could support and diversify the organic vegetable items, providing us with added-value products for organic food supply chains.

scholarly journals Dosage of 2,6-Bis (1.1-Dimethylethyl)-4-Methylphenol (BHT) in the Plant ExtractMesembryanthemum crystallinum

2010 ◽  
Vol 2010 ◽  
pp. 1-5 ◽  
Author(s):  
Bouftira Ibtissem ◽  
Mgaidi Imen ◽  
Sfar Souad
Keyword(s):  
Antioxidant Activity ◽  
Plant Growth ◽  
Plant Extract ◽  
Growth Stage ◽  
Mesembryanthemum Crystallinum ◽  
Growth Stages ◽  
Naturally Occurring ◽  
Plant Growth Stages

A naturally occurring BHT was identified in the leaves of the halophyte plantMesembryanthemum crystallinum. This phenol was extracted in this study by two methods at the different plant growth stages. One of the methods was better for BHT extraction; the concentration of this phenol is plant growth stage dependent. In this study, the floraison stage has the highest BHT concentration. The antioxidant activity of the plant extract was not related to BHT concentration. The higher antioxidant activity is obtained at seedlings stage.

scholarly journals Hormesis with glyphosate depends on coffee growth stage

2013 ◽  
Vol 85 (2) ◽  
pp. 813-822 ◽  
Author(s):  
LEONARDO B. DE CARVALHO ◽  
PEDRO L.C.A. ALVES ◽  
STEPHEN O. DUKE
Keyword(s):  
Plant Growth ◽  
Weed Management ◽  
Growth Stage ◽  
Growth Stages ◽  
Coffee Plant ◽  
Herbicide Application ◽  
Coffee Plantations ◽  
Plant Growth Stages ◽  
Coffee Plants ◽  
Almost All

Weed management systems in almost all Brazilian coffee plantations allow herbicide spray to drift on crop plants. In order to evaluate if there is any effect of the most commonly used herbicide in coffee production, glyphosate, on coffee plants, a range of glyphosate doses were applied directly on coffee plants at two distinct plant growth stages. Although growth of both young and old plants was reduced at higher glyphosate doses, low doses caused no effects on growth characteristics of young plants and stimulated growth of older plants. Therefore, hormesis with glyphosate is dependent on coffee plant growth stage at the time of herbicide application.

scholarly journals Scale-Dependent Effects of Growth Stage and Elevational Gradient on Rice Phyllosphere Bacterial and Fungal Microbial Patterns in the Terrace Field

2022 ◽  
Vol 12 ◽  
Author(s):  
Pei Wang ◽  
Jianping Dai ◽  
Luyun Luo ◽  
Yong Liu ◽  
Decai Jin ◽  
...  
Keyword(s):  
Plant Growth ◽  
Growth Stage ◽  
Elevational Gradient ◽  
Fungal Communities ◽  
Growth Stages ◽  
Strong Positive Correlation ◽  
Elevation Gradients ◽  
Positive Correlation ◽  
Α Diversity ◽  
Phyllosphere Bacteria

The variation of phyllosphere bacterial and fungal communities along elevation gradients may provide a potential link with temperature, which corresponds to an elevation over short geographic distances. At the same time, the plant growth stage is also an important factor affecting phyllosphere microorganisms. Understanding microbiological diversity over changes in elevation and among plant growth stages is important for developing crop growth ecological theories. Thus, we investigated variations in the composition of the rice phyllosphere bacterial and fungal communities at five sites along an elevation gradient from 580 to 980 m above sea level (asl) in the Ziquejie Mountain at the seedling, heading, and mature stages, using high-throughput Illumina sequencing methods. The results revealed that the dominant bacterial phyla were Proteobacteria, Actinobacteria, and Bacteroidetes, and the dominant fungal phyla were Ascomycota and Basidiomycota, which varied significantly at different elevation sites and growth stages. Elevation had a greater effect on the α diversity of phyllosphere bacteria than on that phyllosphere fungi. Meanwhile, the growth stage had a great effect on the α diversity of both phyllosphere bacteria and fungi. Our results also showed that the composition of bacterial and fungal communities varied significantly along elevation within the different growth stages, in terms of both changes in the relative abundance of species, and that the variations in bacterial and fungal composition were well correlated with variations in the average elevation. A total of 18 bacterial and 24 fungal genera were significantly correlated with elevational gradient, displaying large differences at the various growth stages. Soluble protein (SP) shared a strong positive correlation with bacterial and fungal communities (p < 0.05) and had a strong significant negative correlation with Serratia, Passalora, unclassified_Trichosphaeriales, and antioxidant enzymes (R > 0.5, p < 0.05), and significant positive correlation with the fungal genera Xylaria, Gibberella, and Penicillium (R > 0.5, p < 0.05). Therefore, it suggests that elevation and growth stage might alter both the diversity and abundance of phyllosphere bacterial and fungal populations.

scholarly journals Changes in the Content of Some Groups of Phenolic Compounds and Biological Activity of Extracts of Various Parts of Heather (Calluna vulgaris (L.) Hull) at Different Growth Stages

Plants ◽  
2020 ◽  
Vol 9 (8) ◽  
pp. 926
Author(s):  
Victoria Chepel ◽  
Valery Lisun ◽  
Liubov Skrypnik
Keyword(s):  
Antioxidant Activity ◽  
Ascorbic Acid ◽  
Biological Activity ◽  
Phenolic Compounds ◽  
Calluna Vulgaris ◽  
Growth Stages ◽  
Flowering Stage ◽  
Leaf Extracts ◽  
Frap Assay ◽  
Different Growth Stages

Heather (Calluna vulgaris (L.) Hull.) is noted for a diverse chemical composition and a broad range of biological activity. The current study was aimed at monitoring changes in the accumulation of certain groups of phenolic compounds in various organs of heather (leaves, stems, roots, rhizomes, flowers, and seeds) at different growth stages (vegetative, floral budding, flowering, and seed ripening) as well as studying antioxidant (employing the DPPH and FRAP assays) and antibacterial activity of its extracts. The highest total amount of phenolic compounds, tannins, flavonoids, hydroxycinnamic acids, and proanthocyanidins was detected in leaves and roots at all growth stages, except for the flowering stage. At the flowering stage, the highest content of some groups of phenolic compounds (flavonoids, proanthocyanidins, and anthocyanins) was observed in flowers. Highest antioxidant activity was recorded for the flower extracts (about 500 mg of ascorbic acid equivalents per gram according to the DPPH assay) and for the leaf extract at the ripening stage (about 350 mg of ascorbic acid equivalents per gram according to the FRAP assay). Strong correlation was noted between antioxidant activity (DPPH) and the content of anthocyanins (r = 0.75, p ≤ 0.01) as well as between antioxidant activity (FRAP) and the total content of phenolic compounds (r = 0.77, p ≤ 0.01). Leaf extracts and stem extracts turned out to perform antibacterial action against both gram-negative and gram-positive bacteria, whereas root extracts appeared to be active only against B. subtilis, and rhizome extracts against E. coli.

scholarly journals Freeze Dried Acerola (Malpighia emarginata) Pulp and Pomace: Physicochemical Attributes, Phytochemical Content and Stability during Storage

2017 ◽  
Vol 1 (1) ◽  
pp. 17 ◽  
Author(s):  
Francisca Pereira De Moraes ◽  
Aline Cardoso Gonçalves ◽  
Thaís Bezerra Veríssimo Miguel ◽  
Kátia Cristina Borges ◽  
Roberta T P Correia
Keyword(s):  
Antioxidant Activity ◽  
Ascorbic Acid ◽  
Gum Arabic ◽  
Total Phenolic ◽  
Food Ingredients ◽  
Phytochemical Content ◽  
Freeze Dried ◽  
Malpighia Emarginata ◽  
Bioactive Content ◽  
Monomeric Anthocyanins

In this study, acerola pulp and acerola pomace were freeze dried with and without gum Arabic (GA) and analyzed in regard to their physicochemical attributes, bioactive content, and phytochemical stability (total phenolic content, ascorbic acid, total monomeric anthocyanins and antioxidant activity), during storage at 4 oC and 25 oC. The freeze dried products were highly porous and had low water activity (0.273 to 0.300). Freeze dried acerola pulp with GA were the most soluble samples (64.1% to 73.7 %). The addition of GA to freeze dried acerola pomace increased samples hygroscopicity from 4.5% to 11.1%. High ascorbic acid retention in freeze dried acerola pulp was observed for all samples (65.2 % at 25 °C and 88.9% at 4 °C). It was observed that freeze dried acerola pomace showed increased antioxidant activity during storage (101.9% at 4 °C and 135.9% at 25 °C). Our results demonstrate the potential of freeze dried acerola pulp and pomace as novel food ingredients with concentrated phytochemical content and desirable physicochemical attributes. 

Efficacy ofColletotrichum coccodesand Thidiazuron for Velvetleaf (Abutilon theophrasti) Control in Soybean (Glycine max)

1988 ◽  
Vol 2 (4) ◽  
pp. 473-480 ◽  
Author(s):  
Richard H. Hodgson ◽  
Lee A. Wymore ◽  
Alan K. Watson ◽  
Robert H. Snyder ◽  
Anne Collette
Keyword(s):  
Glycine Max ◽  
Plant Growth ◽  
Growth Regulator ◽  
Plant Growth Regulator ◽  
Growth Stage ◽  
Pathogenic Fungus ◽  
Abutilon Theophrasti ◽  
Colletotrichum Coccodes ◽  
Growth Stages ◽  
Wet Weather

The plant pathogenic fungusColletotrichum coccodes(Cc) and the plant growth regulator thidiazuron (TDZ) were evaluated in Maryland and Quebec for velvetleaf control in ‘Williams' and ‘Maple Arrow’ soybean. TDZ was applied at 0, 0.1, 0.2, 0.3, and 0.4 kg ai/ha alone or was combined with Cc at 109spores/m2when velvetleaf was at the 1- to 2-leaf (Trial 1) or 4- to 6-leaf (Trial 2) growth stages. Velvetleaf control increased with TDZ rate, and TDZ combined with Cc further increased control. TDZ reduced velvetleaf biomass and height, and Cc increased velvetleaf mortality. In Quebec, Cc also reduced the biomass of velvetleaf treated in Trial 1 and interacted positively with TDZ at this growth stage. Cc nearly halved the rates of TDZ required for 90 and 75% mortality of velvetleaf treated at the 1- to 2-leaf and 4- to 6-leaf stages to 0.09 and 0.12 kg/ha, respectively, in Quebec. Cc similarly lowered the rate of TDZ required for 75% stand reduction of velvetleaf in Trial 1 to 0.17 kg/ha in Maryland. Cool wet weather in Quebec contrasted with warm, dry weather in Maryland. Soybean biomass and yield were increased significantly by treatment with TDZ plus Cc in Trial 1 at both locations.

Dinitrogen fixation in Phaseolus vulgaris at low temperatures: interaction of temperature, growth stage, and time of inoculation

1982 ◽  
Vol 60 (8) ◽  
pp. 1423-1427 ◽  
Author(s):  
R. J. Rennie ◽  
G. A. Kemp
Keyword(s):  
Phaseolus Vulgaris ◽  
Plant Growth ◽  
Root Growth ◽  
Low Temperatures ◽  
Growth Stage ◽  
Growth Stages ◽  
Trifoliate Leaf ◽  
Temperature Growth ◽  
Southern Alberta ◽  
Plant Growth Stages

Nodulation and N2 fixation have not been reported in beans (Phaseolus vulgaris L.) below a temperature of 13 °C but, in southern Alberta, temperatures at planting may be as low as 10 °C. Two varieties of pea beans, 'Aurora' and 'Kentwood,' were inoculated at three growth stages (seeding, primary leaf horizontal, or first trifoliate leaf open) and grown at 10, 12, 14, or 16 °C. Nodulation and acetylene (C2H2) reduction occurred in both varieties at temperatures as low as 10 °C. At the lower temperatures, cold adaptability of the plant for early root growth determined the ability for nodulation and N2 fixation. At higher temperatures, plant-growth stage was a determining factor. 'Aurora' was superior to 'Kentwood' at 10 °C in nodulation, dry matter (DM), N yield, and N2 fixation because of its tolerance to low temperatures during early root growth. Inoculation with Rhizobium phaseoli at more advanced plant-growth stages decreased the time for nodulation at all four temperatures but resulted in higher yield and more N2 fixation in 'Aurora' only at 14 and 16 °C. At 10 °C, inoculation at seeding was more effective than at the other two growth stages for both varieties. Thus plant growth stages and growth temperature both determined the ability of a bean variety to support N2 fixation at various low temperatures.

scholarly journals Developing Rainfall- and Temperature-Based Models to Describe Infection of Canola Under Field Conditions Caused by Pycnidiospores of Leptosphaeria maculans

2009 ◽  
Vol 99 (7) ◽  
pp. 879-886 ◽  
Author(s):  
Kaveh Ghanbarnia ◽  
W. G. Dilantha Fernando ◽  
Gary Crow
Keyword(s):  
Plant Growth ◽  
Growth Stage ◽  
Leptosphaeria Maculans ◽  
The Other ◽  
Field Conditions ◽  
Maximum Temperature ◽  
Growth Stages ◽  
Upper Limit ◽  
Average Maximum ◽  
Two Stages

Blackleg, also known as Phoma stem canker, caused by Leptosphaeria maculans (Phoma lingam), is one of the most serious diseases of canola worldwide. In this study, the mean disease severity (Ds) and incidence (Di) of canola cv. Westar plants infected at the cotyledon, three-leaf, and six-leaf stages by pycnidiospores of L. maculans were monitored in the greenhouse after infection of the plants under field conditions in two successive years and associated with meteorological data during infection time. Pearson's correlation coefficient showed that total rainfall per week (R) was significantly correlated to Ds on plants infected at the cotyledon, three-leaf, and six-leaf stages, and average maximum temperature per week (Tmax) only showed significant correlation with plants infected at the cotyledon and six-leaf stages. These results also indicated that there is correlation between Di and R for plants infected at all three growth stages. A nonlinear model was developed to evaluate the combined effects of R and Tmax on Ds. The best model comprised monomolecular function and β probability density function for plants infected at the above three growth stages. Parameters, including maximum potential for Ds at a given rainfall (dmax), rate of changes with respect to rainfall (k), constant of integration (B), maximum potential for Ds with respect to Tmax (e), rate of increase with increasing Tmax to optimum (n), and rate of decrease as Tmax increased and passed the optimum Tmax (p), were estimated for plants infected at the above three growth stages. The effect of plant growth stage was characterized by differences in the upper limit parameter a. This parameter was greater for the plants infected at the cotyledon stage than for plants infected at the other two stages. The estimate of parameter k was the same for the plants infected at the cotyledon and three-leaf stages. This parameter was much lower for the plants infected at the six-leaf stage compared with two other stages. The logistic model could describe the disease incidence with respect to R slightly better than the other two models in the plants infected at all three growth stages. Based on the model, upper-limit estimate (dmax) was ≈100, 94.4, and 88.8% in the plants infected at cotyledon, three-leaf, and six-leaf stages, respectively. Di increased until rainfall reached ≈18, 10, and 13 mm/week and became constant in the plants at cotyledon, three-leaf, and six-leaf stages, respectively. Effects of plant growth stage on the rate of change with respect to R (parameter k) were lower in the plants infected at cotyledon than at the other two stages. The accuracy of the nonlinear models suggests that they could be used to develop a comprehensive model to evaluate epidemics of blackleg based on pycnidiospores as sources of inoculum. However, additional years of data collection should improve model fit and evaluation of introduced models and contribute to the development of a more robust predictive model.

scholarly journals Effect of Selenium Application and Growth Stage at Harvest on Hydrophilic and Lipophilic Antioxidants in Lamb’s Lettuce (Valerianella locusta L. Laterr.)

Plants ◽  
2021 ◽  
Vol 10 (12) ◽  
pp. 2733
Author(s):  
Liubov Skrypnik ◽  
Tatiana Styran ◽  
Tamara Savina ◽  
Nadezhda Golubkina
Keyword(s):  
Antioxidant Activity ◽  
Phenolic Compounds ◽  
Growth Stage ◽  
Dry Weight ◽  
Growth Stages ◽  
Chlorophyll B ◽  
Cultivation Conditions ◽  
Lipophilic Compounds ◽  
Food Value ◽  
Β Carotene

Lamb’s lettuce (Valerianella locusta L. Laterr.) is a leafy green vegetable that is rich in various biological active compounds and is widely used in ready-to-eat salads. The cultivation conditions and growth stage could affect the secondary metabolism in plants and thereby modify their food value. In the present study, the effect of selenium (Se) application in various concentrations (5.0, 10.0, and 20.0 µM) on the contents of Se, phenolic compounds, vitamin C, carotenoids, chlorophylls, and antioxidant activity of hydrophilic and lipophilic extracts of lamb’s lettuce harvested at three growth stages (38, 52, and 66 days after sowing (DAS)) was studied. Se application significantly increased the Se concentration in the shoots (up to 124.4 μg g−1 dry weight), as well as the contents of chlorogenic acid, total flavonoids, total phenolics, ascorbic acid, chlorophyll b, and the antioxidant activity of hydrophilic and lipophilic extracts. A higher content of phenolic compounds and higher antioxidant activity of hydrophilic extracts was observed at the first growth stage (38 DAS). On the contrary, higher contents of lipophilic compounds (chlorophyll a, chlorophyll b, lutein, β-carotene) and higher antioxidant activity of lipophilic extracts were found for shoots harvested at later stages (52 and 66 DAS).

scholarly journals Phenolics and Carotenoids Contribute to Antioxidant Activity in Navel Orange

HortScience ◽  
2006 ◽  
Vol 41 (4) ◽  
pp. 1000A-1000
Author(s):  
G.K. Jayaprakasha ◽  
Clark Wilson ◽  
Bhimanagouda S. Patil
Keyword(s):  
Antioxidant Activity ◽  
Ascorbic Acid ◽  
Ethyl Acetate ◽  
Antioxidant Capacity ◽  
Fruits And Vegetables ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Navel Orange ◽  
Scavenging Activity ◽  
Freeze Dried

Consumption of fruits and vegetables has been associated with reduced risk of disease, such as neurodegenerative disorders and certain forms of cancer, and aging. Antioxidants prevent the damage to macromolecules and cells by interfering with the free radicals. Several natural compounds that posses antioxidant activity have been reported from plant sources and are commercially promoted as nutraceuticals. Citrus fruits contain certain bioactive compounds such as phenolics, flavonoids, limonoids, carotenoids, and ascorbic acid. In this context, navel oranges were freeze-dried and extracted with five different solvents, such as hexane, ethyl acetate, acetone, methanol, and 8 methanol: 2 water. The extracts were dried under vacuum and screened for their radical scavenging activity using the 1,1-diphenyl-2-picrylhydrazyl method at 250, 500, and 1000 ppm. The methanol: water and methanol extracts of navel orange were found to be maximum (92.9%) and minimum (63.89%) radical scavenging activity at 1000 ppm. Furthermore, the antioxidant capacity of all extracts was assayed through the phosphomolybdenum method and expressed as equivalent to ascorbic acid (μmol/g of the extract). The order of antioxidant capacity for navel orange extracts was found to be ethyl acetate > acetone > methanol: water > methanol > hexane. It seems that the antioxidant capacity of the extracts is in accordance with the amount of phenolics/lycopene present in each fraction and may provide a good source of antioxidants. This project is based upon work supported by the USDA–CSREES under Agreement USDA IFAFS #2001-52102-02294 and USDA #2005-34402-14401 “Designing Foods for Health” through the Vegetable and Fruit Improvement Center.

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