Metabolomic, Ionomic and Microbial Characterization of Olive Xylem Sap Reveals Differences According to Plant Age and Genotype

Vascular pathogens are the causal agents of main diseases threatening the health and growth of olive crops worldwide. The use of endophytic microorganisms represents a challenging and promising strategy for management of vascular diseases in olive. Although current research has been focused on analyzing the structure and diversity of the endophytic microbial communities inhabiting the olive xylem, the characterization of this ecological niche has been overlooked and to date remain unexplored, despite that the characterization of the xylem sap composition is essential to unravel the nutritional requirements of xylem-limited microorganisms. In this study, branches from plantlets and adult olive trees of cultivars Picual and Arbequina were selected to characterize the chemical and microbial composition of olive xylem sap extracted using a Scholander pressure chamber. Metabolome and ionome analyses of xylem sap were performed by proton nuclear magnetic resonance (NMR) spectroscopy-based and by inductively coupled plasma with optical emission spectroscopy (ICP-OES), respectively. Olive xylem sap metabolites included a higher relative percentage of sugars (54.35%), followed by alcohols (28.85%), amino acids (8.01%), organic acids (7.68%), and osmolytes (1.12%). Within each of these groups, the main metabolites in the olive xylem sap were mannitol, ethanol, glutamine, acetic acid, and trigonelline, whereas K and Cl− were the main element and inorganic anion, respectively. Metabolomic profile varied when comparing olive plant age and genotype. The levels of glucose, fructose, sucrose and mannitol, choline, B and PO43− were significantly higher in adult trees than in plantlets for both olive genotypes, whereas NO3− and Rb content showed the opposite behavior. On the other hand, levels of aspartic acid, phenylalanine, and Na were significantly higher in ‘Picual’ than in ‘Arbequina’, whereas Fe showed the opposite behavior, but only for adult trees. Microbiome composition identified Firmicutes (67%), Proteobacteria (22%) and Actinobacteriota (11%) as the main phyla, while at the genus level Anoxybacillus (52%), Cutibacterium (7%), Massilia (6%), and Pseudomonas (3%) were the most representative. Both non-supervised hierarchical clustering analysis and supervised PLS-DA analysis differentiated xylem sap chemical and microbial composition first, according to the age of the plant and then by the olive genotype. PLS-DA analysis revealed that B, ethanol, Fe, fructose, glucose, mannitol, sucrose, and Sr, and Anoxybacillus, Cutibacterium, and Bradyrhizobium were the most significant chemical compounds and bacterial genera, respectively, in the discrimination of adult olive trees and plantlets. Knowledge of the chemical composition of xylem sap will lead to a better understanding of the complex nutritional requirements of olive xylem-inhabiting microorganisms, including vascular pathogens and their potential antagonists, and may allow the better design of artificial growing media to improve the culturing of the olive microbiome.

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Metabolomic Characterization of Olive Xylem Sap Reveals Differences According to Plant Age and Genotype

10.20944/preprints202103.0210.v1 ◽

2021 ◽

Author(s):

Manuel Anguita-Maeso ◽

Carmen Haro ◽

Miguel Montes-Borrego ◽

Leonardo De La Fuente ◽

Juan A. Navas-Cortés ◽

...

Keyword(s):

Chemical Composition ◽

Xylem Sap ◽

Plant Age ◽

Proton Nuclear Magnetic Resonance ◽

Main Element ◽

Nutritional Requirements ◽

Adult Trees ◽

Opposite Behavior ◽

Vascular Pathogens

Vascular pathogens are the causal agents of main diseases threatening the health and growth of olive crops worldwide. The use of endophytic microorganisms represents a challenging and promising strategy for management of vascular diseases in olive. Although current research has been focused on analyzing the structure and diversity of the endophytic microbial communities inhabiting the olive xylem, the characterization of this ecological niche has been overlooked and to date remain unexplored, despite that the characterization of the xylem sap composition is essential to unravel the nutritional requirements of xylem-limited microorganisms. In this study, branches from plantlets and adult olive trees of cultivars ‘Picual’ and ‘Arbequina' were selected to characterize the chemical composition of olive xylem sap extracted using a Scholander pressure chamber. Metabolome and ionome analyses of xylem sap were performed by proton nuclear magnetic resonance (NMR) spectroscopy-based and by inductively coupled plasma with optical emission spectroscopy (ICP-OES), respectively. Olive xylem sap metabolites included a higher relative percentage of sugars (54.35%), followed by alcohols (28.85%), amino acids (8.01%), organic acids (7.68%) and osmolytes (1.12%). Within each of these groups, the main metabolites in the olive xylem sap were mannitol, ethanol, glutamine, acetate and trigonelline, whereas K and Cl- were the main element and inorganic anion, respectively. Metabolomic profile varied when comparing olive plant age and genotype. The levels of glucose, fructose, sucrose and mannitol, choline, B and PO43 were significantly higher in adult trees than in plantlets for both olive genotypes, whereas NO3- and Rb content showed the opposite behavior. On the other hand, levels of aspartate, phenylalanine and Na were significantly higher in ‘Picual’ than in ‘Arbequina’ whereas Fe showed the opposite behavior but only for adult trees. Non-supervised hierarchical clustering analysis separated xylem sap composition firstly according to the plant age and then by the olive cultivar. Supervised PLS-DA analysis revealed that B, ethanol, Fe, Fructose, glucose, mannitol, sucrose and Sr were the most significative compounds discriminating adult trees from plantlets, whereas asparagine, aspartate, glutamate and phenylalanine or aspartate, arginine, ethanol and Sr were the most contributory compounds in the discrimination of both olive genotypes for adult trees or plantlets, respectively. Knowledge of the chemical composition of xylem sap will lead to a better understanding of the complex nutritional requirements of olive xylem-inhabiting microorganisms, including its vascular pathogens, and would allow the design of artificial growing media to improve culturing the olive microbiome.

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Analytical Characterization of Water-Soluble Constituents in Olive-Derived By-Products

Foods ◽

10.3390/foods10061299 ◽

2021 ◽

Vol 10 (6) ◽

pp. 1299

Author(s):

Pablo Doménech ◽

Aleta Duque ◽

Isabel Higueras ◽

José Luis Fernández ◽

Paloma Manzanares

Keyword(s):

Olive Tree ◽

Mediterranean Area ◽

Water Soluble ◽

Olive Pomace ◽

Olive Leaves ◽

Olive Stone ◽

Olive Trees ◽

Tree Pruning ◽

By Products

Olive trees constitute one of the largest agroindustries in the Mediterranean area, and their cultivation generates a diverse pool of biomass by-products such as olive tree pruning (OTP), olive leaves (OL), olive stone (OS), and extracted olive pomace (EOP). These lignocellulosic materials have varying compositions and potential utilization strategies within a biorefinery context. The aim of this work was to carry out an integral analysis of the aqueous extractives fraction of these biomasses. Several analytical methods were applied in order to fully characterize this fraction to varying extents: a mass closure of >80% was reached for EOP, >76% for OTP, >65% for OS, and >52% for OL. Among the compounds detected, xylooligosaccharides, mannitol, 3,4-dihydroxyphenylglycol, and hydroxytyrosol were noted as potential enhancers of the valorization of said by-products. The extraction of these compounds is expected to be more favorable for OTP, OL, and EOP, given their high extractives content, and is compatible with other utilization strategies such as the bioconversion of the lignocellulosic fraction into biofuels and bioproducts.

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Processing Effect and Characterization of Olive Oils from Spanish Wild Olive Trees (Olea europaea var. sylvestris)

Molecules ◽

10.3390/molecules26051304 ◽

2021 ◽

Vol 26 (5) ◽

pp. 1304

Author(s):

Francisco Espínola ◽

Alfonso M. Vidal ◽

Juan M. Espínola ◽

Manuel Moya

Keyword(s):

Phenolic Compounds ◽

Volatile Compounds ◽

Olive Tree ◽

Extraction Yield ◽

Point Of View ◽

Human Consumption ◽

Analytical Point ◽

Wild Olive ◽

Olive Trees

Wild olive trees have important potential, but, to date, the oil from wild olives has not been studied significantly, especially from an analytical point of view. In Spain, the wild olive tree is called “Acebuche” and its fruit “Acebuchina”. The objective of this work is to optimize the olive oil production process from the Acebuchina cultivar and characterize the oil, which could be marketed as healthy and functional food. A Box–Behnken experimental design with five central points was used, along with the Response Surface Methodology to obtain a mathematical experimental model. The oils from the Acebuchina cultivar meet the requirements for human consumption and have a good balance of fatty acids. In addition, the oils are rich in antioxidants and volatile compounds. The highest extraction yield, 12.0 g oil/100 g paste, was obtained at 90.0 min and the highest yield of phenolic compounds, 870.0 mg/kg, was achieved at 40.0 °C, and 90.0 min; but the maximum content of volatile compounds, 26.9 mg/kg, was obtained at 20 °C and 30.0 min. The oil yield is lower than that of commercial cultivars, but the contents of volatile and phenolic compounds is higher.

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Towards In Vivo Monitoring of Ions Accumulation in Trees: Response of an in Planta Organic Electrochemical Transistor Based Sensor to Water Flux Density, Light and Vapor Pressure Deficit Variation

Applied Sciences ◽

10.3390/app11114729 ◽

2021 ◽

Vol 11 (11) ◽

pp. 4729

Author(s):

Davide Amato ◽

Giuseppe Montanaro ◽

Filippo Vurro ◽

Nicola Coppedé ◽

Nunzio Briglia ◽

...

Keyword(s):

Vapor Pressure ◽

Flux Density ◽

Precision Agriculture ◽

Vapor Pressure Deficit ◽

Accumulation Rate ◽

Xylem Sap ◽

Water Flux ◽

Electrochemical Transistor ◽

Olive Trees

Research on organic electrochemical transistor (OECT) based sensors to monitor in vivo plant traits such as xylem sap concentration is attracting attention for their potential application in precision agriculture. Fabrication and electronic aspects of OECT have been the subject of extensive research while its characterization within the plant water relation context deserves further efforts. This study tested the hypothesis that the response (R) of an OECT (bioristor) implanted in the trunk of olive trees is inversely proportional to the water flux density flowing through the plant (Jw). This study also examined the influence on R of vapor pressure deficit (VPD) as coupled/uncoupled with light. R was hourly recorded in potted olive trees for a 10-day period concomitantly with Jw (weight loss method). A subgroup of trees was bagged in order to reduce VPD and in turn Jw, and other trees were located in a walk-in chamber where VPD and light were independently managed. R was tightly sensitive to diurnal oscillation of Jw and at negligible values of Jw (late afternoon and night) R increased. The bioristor was not sensitive to the VPD per se unless a light source was coupled to trigger Jw. This study preliminarily examined the suitability of bioristor to estimate the mean daily nutrients accumulation rate (Ca, K) in leaves comparing chemical and sensor-based procedures showing a good agreement between them opening new perspective towards the application of OECT sensor in precision agricultural cropping systems.

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Study of the nutritional dynamic in olive leaves: periods of analytical stability

Spanish Journal of Soil Science ◽

10.3232/sjss.2017.v7.n1.04 ◽

2017 ◽

Vol 7 ◽

Author(s):

Juana Nieto ◽

Antonio García-Fuentes ◽

Llenalia M. García ◽

Emilia Fernández-Ondoño

Keyword(s):

Nutritional Deficiencies ◽

Winter Period ◽

Olive Leaves ◽

Nitrogen And Phosphorus ◽

Analytical Stability ◽

Opposite Behavior ◽

Zinc Concentrations ◽

Calcium Magnesium ◽

Olive Trees ◽

One Year

The aim of this study was to establish the evolution curves of macro- and micro-nutrients in olive leaves and the periods of time in which they are statistically stable and can be considered suitable for a nutritional diagnosis. The study was carried out in two farms of the province of Jaén (Andalucia, Southern Spain), with olive trees of the Picual variety, under an irrigation regime and no nutritional deficiencies over the entire year. The evolution curves of each nutrient had great similarities when comparing between farms and between periods. Only in some periods the farm “Poco Humo” had higher concentrations, probably due to the most favorable edaphic characteristics of this farm. Nitrogen and phosphorus showed minimum concentrations when the leaves were young and when they were one year old, and maximum concentrations during the winter. Potassium and boron showed higher concentrations when the leaves were young, and the concentrations decreased throughout the first year of life. Concentrations of calcium, magnesium and manganese had the opposite behavior: these accumulated in leaf until reaching maximum values in winter and then remained stable, with some oscillations but without statistically significant differences. No changes were observed in the zinc concentrations in the sampled periods. Periods of at least two consecutive months without statistically significant differences were found during the winter period for all elements except nitrogen. Analytical stability was observed from the second fortnight of May till the first fortnight of August for all elements except phosphorus and boron.

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Activated sludge population dynamics

Water Science & Technology ◽

10.2166/wst.1994.0556 ◽

1994 ◽

Vol 30 (11) ◽

pp. 159-169 ◽

Cited By ~ 6

Author(s):

Jiri Wanner

Keyword(s):

Population Dynamics ◽

Activated Sludge ◽

Organic Pollution ◽

Paper Briefly ◽

Microbial Composition ◽

Selection Mechanisms ◽

Sludge Settling

The paper briefly surveys topics covered by the IAWQ Specialist Group on Activated Sludge Population Dynamics. The activated sludge population dynamics has been formulated as a branch of water science and technology concerned with phenomena governing the relationships between activated sludge microorganisms and their functions. The characterization of organic pollution fractions in wastewaters according to their rate of biodegradation has been discussed and the role of wastewater as an inoculum stressed. The characterization of activated sludge biomass has been evaluated from two viewpoints: grouping according to metabolic abilities and identification and classification of activated sludge microorganisms. The basic selection mechanisms influencing the microbial composition of activated sludge have been described. The problems with activated sludge settling and thickening properties have been mentioned as a typical example of applied population dynamics research.

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Hydroxyapatite Production from Cuttlebone as Bone Scaffold Material Preparations

Jurnal Pengolahan Hasil Perikanan Indonesia ◽

10.17844/jphpi.v22i1.25869 ◽

2019 ◽

Vol 22 (1) ◽

pp. 1 ◽

Cited By ~ 1

Author(s):

Krisman Umbu Henggu ◽

Bustami Ibrahim ◽

Pipih Suptijah

Keyword(s):

Calcination Temperature ◽

Physicochemical Characteristics ◽

Main Element ◽

Bone Scaffold ◽

Scaffold Material ◽

Inorganic Element ◽

Material Preparations ◽

Calcination Treatment ◽

High Absorption

The increasing production of cuttlefish has been associated with the increasing of by-product waste particularly cuttlebone. Cuttlebone is known to contain an inorganic element in form of calcium carbonate<br />(CaCO3) which can be utilized as a source of calcium oxide (CaO) for hydroxyapatite synthesis. This study was aimed to determine the physicochemical characteristics of the cuttlebone and the optimum calcination temperature for CaO extraction and hydroxyapatite synthesis. This study was divided into three steps. Firstly, analysis of the cuttlebone physicochemical properties; secondly, extraction and characterization of the CaO with different calcination temperature (500°C, 600°C, 700°C for 6 hours); and thirdly, hydroxyapatite synthesis using a combination of hydrothermal method at 200°C 6 hours and different calcination treatments (800°C, 900°C, 1,000°C for 1 hour). The results showed that the cuttlebone contained moisture 3.54±0.11%,<br />lipid 0.32±0.19%, protein 4.78±0.23%, carbohydrate 5.29±0.02%, and ash 89.61±0.26. The main element of the ash was CaCO3 aragonite characterized by the high absorption at wavelengths of 1,795; 1,507;<br />1,083; 871; 713 and 700 cm-1. The calcination treatment of 700°C produced the highest amount of CaO. The hydroxyapatite produced with a combination of hydrothermal and calcination temperature 1,000°C<br />had calcium phosphate ratio (Ca/P) 1.66, crystalline level 90.10%, amorphous level 9.90% and particles morphology of rod-shaped.

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Development and characterization of microsatellite loci for Cedrela fissilis Vell (Meliaceae), an endangered tropical tree species

Silvae Genetica ◽

10.1515/sg-2014-0031 ◽

2014 ◽

Vol 63 (1-6) ◽

pp. 240-243 ◽

Cited By ~ 4

Author(s):

F. B. Gandara ◽

E. V. Tambarussi ◽

Alexandre Magno Sebbenn ◽

E. M. Ferraz ◽

M. A. Moreno ◽

...

Keyword(s):

Genomic Library ◽

Population Level ◽

Fixation Index ◽

Allele Number ◽

Neotropical Tree ◽

Expected Heterozygosity ◽

Adult Trees ◽

Ssr Loci ◽

Polymorphic Microsatellite

Abstract The timber of the Neotropical tree Cedrela fissilis is used in construction, shipbuilding, carpentry and for medical purposes. In this study, polymorphic microsatellite (SSR) markers derived from an enriched genomic library were characterized using 120 adult trees from four different C. fissilis populations. No substantial genotypic linkage disequilibrium was detected among all possible pairs of SSR loci. The number of alleles per locus ranged from 2 to 20, the average allele number ranged from 8 to 9.7, depending on the population. The observed heterozygosity among the different SSR loci varied from 0.0 to 1.00 , the expected heterozygosity varied from 0.07 to 0.95 On the population level, the average observed and expected heterozygosities ranged from 0.50 to 0.63 and from 0.64 to 0.70, respectively. The average fixation index among populations ranged from 0.09 to 0.24. Thus, the SSR loci revealed high poly - morphism rates and can be used to study the genetic diversity,structure, mating system, and gene flow in C. fissilis.

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The formation of Hfr strains inEscherichia coliK12

Genetics Research ◽

10.1017/s0016672300010296 ◽

1967 ◽

Vol 9 (1) ◽

pp. 35-47 ◽

Cited By ~ 32

Author(s):

Paul Broda

Keyword(s):

Growth Rate ◽

Bacterial Chromosome ◽

Nutritional Requirements ◽

Isolation And Characterization

The isolation and characterization of an isogenic series of twelve Hfr strains is described. From their points of origin it was concluded that the sex factor integrates at a limited number of sites on the bacterial chromosome. Although the nutritional requirements of these Hfr strains were similar to those of the parent F+strain, it was observed that one group had a markedly slower growth rate. The relevance of these observations to theories on the nature of F+fertility and on the formation of Hfr strains is discussed.

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Characterization of the Residual Stresses in Plastically Deformed Ferrite-Martensite Steels Using Barkhausen Noise Measurements

Materials Science Forum ◽

10.4028/www.scientific.net/msf.500-501.655 ◽

2005 ◽

Vol 500-501 ◽

pp. 655-662 ◽

Cited By ~ 1

Author(s):

Xavier Kleber ◽

Aurélie Hug-Amalric ◽

Jacques Merlin

Keyword(s):

Plastic Deformation ◽

Tensile Stress ◽

Residual Stresses ◽

Barkhausen Noise ◽

Opposite Behavior ◽

Noise Measurements ◽

Two Phases ◽

Noise Signature ◽

Compressive Residual Stresses

In this work, we show that the measurement of the Barkhausen noise allows the residual stresses in each of the two phases of ferrite-martensite steels to be characterized. We have first studied the effect of a tensile and a compressive stress on the Barkhausen noise signature. We observed that for a ferrite-martensite steel, the application of a tensile stress increases the Barkhausen activity of the martensite and ferrite phases, whereas a compressive one reduces it. In a second time, we induced residual stresses by applying a plastic deformation to ferrite-martensite steels. After a tensile plastic deformation, we observed that (i) compressive residual stresses appear in ferrite, and (ii) tensile residual stresses appear in martensite. An opposite behavior is observed after a compressive plastic deformation. These results show that the Barkhausen noise measurement makes it possible to highlight in a nondestructive way the distribution of the stresses in each of the two phases of a ferrite-martensite steel. This result could be used to characterize industrial Dual- Phases steels that are plastically deformed during mechanical processes.

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