scholarly journals Studying the Effects of Collagenase (Type 1) on the Collagen in Woody Breast Meat

Animals ◽  
2020 ◽  
Vol 10 (9) ◽  
pp. 1602
Author(s):  
Amit Morey ◽  
Meredith Lane Johnson ◽  
Jasmine Kataria ◽  
John Michael Gonzalez
Keyword(s):  
Type I ◽  
Breast Meat ◽  
Water Addition ◽  
Insoluble Collagen ◽  
Honestly Significant Difference ◽  
Significant Difference ◽  
Major Breast ◽  
One Way Anova ◽  
Control Samples

Abnormal collagen infiltration in the Pectoralis major, breast muscle, of fast-growing big broilers has led to woody breast (WB) myopathy resulting in meat quality issues. Mechanisms to degrade the collagen were investigated to potentially improve WB texture. Freshly deboned WB fillets (n = 5 per trial; 3 trials) were ground and divided in to 25 g portions. Aqueous collagenase Type I solution (1 mL) from concentrations of 2.5, 5, and 10 mg/mL were incorporated in ground WB samples (n = 3 samples/treatment × 3 trials). Ground WB with 1 mL water acted as a control. All the samples were placed at 4 °C for 24 h and frozen at −80 °C. Control samples without any treatment or water addition (n = 3/trial) were frozen immediately upon grinding. Data collected on total (TC), soluble (SC), and insoluble collagen (IC) content was analyzed using one-way ANOVA with Tukey’s honestly significant difference (HSD) (p ≤ 0.05). Fresh WB fillets had TC, SC, and IC content of 19.5, 4.9, and 14.6 mg/g, respectively. The addition of collagenase decreased (p ≤ 0.05) the IC to 5.8 mg/g in the 10 mg/mL treatment after 24 h. Converting IC to SC using collagenase can potentially help the poultry industry to reduce WB toughness.

scholarly journals Quality of Life in Romanian Children with Type 1 Diabetes: A Cross-Sectional Survey Using an Interdisciplinary Healthcare Intervention

Healthcare ◽  
2020 ◽  
Vol 8 (4) ◽  
pp. 382
Author(s):  
Constanta Urzeală ◽  
Aura Bota ◽  
Silvia Teodorescu ◽  
Mihaela Vlăiculescu ◽  
Julien S Baker ◽  
...  
Keyword(s):  
Quality Of Life ◽  
Type 1 Diabetes ◽  
Well Being ◽  
Healthcare Intervention ◽  
Type I ◽  
Cross Sectional ◽  
Significant Difference ◽  
Interdisciplinary Healthcare

Background: The purpose of this study was to assess the quality of life in Romanian type 1 diabetes mellitus (T1DM) children attending an early interdisciplinary healthcare intervention. Hypothesis: engaging T1DM children in leisure sports leads to a better quality of life. Methods: This research embeds a cross-sectional observational study, incorporating some clinical characteristics relevant for diabetes management. The Kidscreen 27 questionnaire was issued to 100 T1DM children aged between 7 and 17 years. Parents completed the questionnaire. All subjects received interdisciplinary healthcare in the previous year. Statistics were performed using SPSS, v20. The required sample size of 100 subjects was obtained with a confidence interval of 95% and a sampling error of 0.009. The tests were two-sided, with a type I error set at 0.05. Results: Subjects reached an increased level of physical well-being, psychological well-being, autonomy, parent relationships, peer and social support, and school inclusion. There was a significant difference (p < 0.05) between children who practice leisure activities and children who only participated in physical education (PE) classes, regarding their physical well-being (t = 2.123). ANOVA demonstrated significant differences between age groups regarding physical well-being. Conclusion: The interdisciplinary healthcare intervention increased the efficiency of T1DM management with positive effects on life quality.

Glycemic disorders in melanoma patients treated with anti-PD1.

2017 ◽  
Vol 35 (15_suppl) ◽  
pp. 9525-9525
Author(s):  
Quentin Magis ◽  
Caroline Gaudy Marqueste ◽  
Sandrine Monestier ◽  
Anderson Loundou ◽  
Marie-Aleth Richard ◽  
...  
Keyword(s):  
Clinical Trial ◽  
Weight Loss ◽  
Type 1 Diabetes ◽  
Type I ◽  
Significant Difference ◽  
Fulminant Diabetes ◽  
Diabetes Development ◽  
Melanoma Patients ◽  
Rare Group

9525 Background: Anti-PD1 are now the backbone of immunotherapy (IT) of metastatic melanoma (MM). Although they are overall well- tolerated, a number of severe immune-related adverse events (IRAE) have been described, among which type 1 diabetes. We observed 3 cases of fulminant diabetes (FD) in our center, and also had the impression that diabetics patients became more difficult to manage when receiving anti-PD1. Methods: Retrospective analysis of blood glucose samples collected before, during and after anti-PD1 treatment (trt ) in all mm patients (pts) receiving anti-PD1 in our department over a 36-month period. Study of FD cases observed. Results: A total of 163 pts were treated with 1920 cures of anti-PD1 including 27 treated within clinical trials. Anti-PD1 was the 1st line of IT in 70% of cases. As a whole, 1470 glycaemia were available. There was no significant difference between the median pre and post-trt glycaemia (5.37 +/-1.6 vs 5.6 +/-1.3 mmol/L (p = 0.033)). In the 28 pts with a type I (n = 0) or II (n = 28) diabetes prior to trt, there was very slight drift toward an increase of glycaemia along with the successive trt infusions (+0.05mmol/L/Cure, p = 0.004 with linear regression tendency test) .Three pts (1.84%) developed a FD revealed by a severe episode of ketosis with acute polyuria polydipsia, hyperglycaemia until 50mmol/L and weight loss. Two additional cases of FD were observed in pts treated within clinical trial comparing anti-PD1 with anti-CTLA4 in adjuvant and metastatic situation (imputability of anti-PD1 likely but uncertain until unblinding). None of these pts had any glucose increase in weeks prior to FD diagnosis. Four out of 5 FD cases had an HLA group at risk for type 1 diabetes development (HLA DRB3/4), a rare group in general population (1%). Conclusions: We could not document any systematic tendency to glycemic disorder in mm pts treated by anti-PD1. In diabetic pts prior to trt, a slight drift toward increase of glycaemia may be explained by other interfering factors (diet, metastatic disease itself, corticosteroids, anxiety etc). FD is not exceptional (2% of patients in our series) and does not seem to be announced by any minor preliminary glycemic disorder. Despite apparently stochastic onset, FD may be associated with HLA DRB3/4 subgroup.

scholarly journals Carcass Production and Meat Tenderness Characteristics of Culled Quail Fed with Azolla microphylla Flour Supplemented Basal Feed

2018 ◽  
Vol 42 (4) ◽  
Author(s):  
Elly Tugiyanti ◽  
Soegeng Herijanto
Keyword(s):  
Analysis Of Variance ◽  
Fat Content ◽  
Collagen Content ◽  
Meat Tenderness ◽  
Breast Meat ◽  
Azolla Microphylla ◽  
Randomized Design ◽  
Honestly Significant Difference ◽  
Significant Difference ◽  
Materials Used

The aim of this research was to understand the effect of Azolla microphylla flour supplementation in the basal feed on carcass production and meat tenderness characteristics of culled quail. The materials used in this research were 100 culled quails at 48-week-old age and commercial quail feed. The research was conducted as an experimental research and used Completely Randomized Design. Four treatments were done in this research, which was basal feed without Azolla microphylla flour supplementation; basal feed supplemented with 1% Azolla microphylla flour; basal feed supplemented with 2% Azolla microphylla flour, and basal feed supplemented with 3% Azolla microphylla flour. Each treatment was done 4 times with each fed into 5 quails. The observed variables include carcass production (carcass percentage, breast meat percentage, thigh meat percentage, back meat percentage, and wing meat percentage) and meat tenderness characteristics (meat fat content, collagen content, pH and meat tenderness). The obtained data were then analyzed by analysis of variance and if the result showed a significant effect, further analysis will be done by honestly significant difference test. The analysis of variance showed that Azolla microphylla flour supplementation showed significant effect (P<0.05) on the carcass percentage, breast meat percentage, meat fat content, collagen content, and meat tenderness but showed no significant effect (P>0.05) on the thigh meat percentage, back meat percentage, wings meat percentage and meat pH. The research concluded that Azolla microphylla flour supplementation in the basal feed will increase carcass production and meat tenderness of culled quail meat at maximum 2% of supplementation level.    

scholarly journals Multiple comparison test by Tukey’s honestly significant difference (HSD): Do the confident level control type I error

2021 ◽  
Vol 6 (1) ◽  
pp. 59-65
Author(s):  
Anita Nanda ◽  
Dr. Bibhuti Bhusan Mohapatra ◽  
Abikesh Prasada Kumar Mahapatra ◽  
Abiresh Prasad Kumar Mahapatra ◽  
Abinash Prasad Kumar Mahapatra
Keyword(s):  
Type I Error ◽  
Multiple Comparison ◽  
Type I ◽  
Level Control ◽  
Comparison Test ◽  
Multiple Comparison Test ◽  
Honestly Significant Difference ◽  
Significant Difference

scholarly journals Molecular and Structural Evaluation of Dentin Caries-Like Lesions Produced by Different Artificial Models

2013 ◽  
Vol 24 (6) ◽  
pp. 610-618 ◽  
Author(s):  
Larissa Ferreira Pacheco ◽  
Efani Caroline de Freitas Banzi ◽  
Eliana Rodrigues ◽  
Luis Eduardo Silva Soares ◽  
Fernanda Miori Pascon ◽  
...  
Keyword(s):  
Surface Hardness ◽  
Collagen Type I ◽  
Sem Analysis ◽  
Type I ◽  
Cross Sectional ◽  
Caries Lesion ◽  
Dentin Caries ◽  
Significant Difference ◽  
One Way Anova ◽  
Ft Raman

This study evaluated structural and molecular issues of dentin caries-like lesions produced by different artificial models (ACL) compared with natural caries lesions (NCL). One hundred twenty-four sound occlusal dentin blocks and 47 carious blocks were obtained and surface hardness was analyzed (SH1). They were assigned to groups according to ACL: GB: Biological; GC: Chemical; GIS: In situ; GNC: natural caries (control). Blocks from groups 1, 2 and 3 were submitted to caries lesion induction. NCL and ACL blocks were submitted to surface hardness (SH 2), FT-Raman and µEDXRF analysis. All blocks were longitudinally sectioned and one of the halves was submitted to cross-sectional hardness (CSH) and the other to SEM analysis. SH1 and SH2 data were submitted to t test (unpaired and paired, respectively), CSH and SEM data to two-way and one-way ANOVA respectively, and Tukey and t tests, respectively (p<0.05). Data from FT-Raman/µEDXRF were submitted to one-way ANOVA and Dunnett multiple-comparisons test (a=0.05). GB and GNC showed lowest SH2 values that were significantly different from GC and GIS. Regarding CSH, GB and GNC showed no significant difference between them. SEM showed similar caries lesion depth for GB and GNC, being significantly higher than for GC and GIS. µEDXRF showed similar values of calcium and phosphate for GB and GNC; GNC values were significantly different from GIS. No significant difference was found among the groups concerning phosphate, carbonate and CH bonds values. For collagen type I, GC values were significantly different compared to other groups. It may be concluded that caries-like lesions produced by GB were the closest model to NCL.

scholarly journals AKTIVITAS FISIK MAKSIMAL AKUT (ACUTE OVERTRAINING) MENYEBABKAN KERUSAKAN SEL β PANKREAS MENCIT

2015 ◽  
Vol 7 (2) ◽  
Author(s):  
Ferbian M. Siswanto ◽  
Boedi P. Yenniastuti ◽  
Tri A. Putra ◽  
I Made Kardena
Keyword(s):  
Oxidative Stress ◽  
Recovery Period ◽  
The Body ◽  
Physical Work ◽  
Type I ◽  
Β Cells ◽  
Randomized Design ◽  
Significant Difference ◽  
Completely Randomized Design ◽  
One Way Anova

Abstract: Excessive physical activity can cause oxidative stress characterized by increased free radicals in the body. This oxidative stress will cause damages to a variety of cells, inter alia β cells of islets of Langerhans in pancreas. This study aimed to determine that acute overtraining could increase the risk of type I diabetes mellitus through induction of pancreatic β cell damages. This study used a completely randomized design with total samples of 36 mice divided into 3 groups. Group P0 was the group of mice without treatment; group P1 was treated with overworking and a recovery period of 48 hours; and group P2 was treated with overworking and a recovery period of 24 hours. After 14 days of treatment, the pancreas of all groups were taken for histological examination using Gomori chrome hematoxylin phloxine B staining. The descriptive analysis showed that the pancreatic tissues of groups P1 and P2 were morphologically damaged compared to group P0. The results of quantitative observations were analyzed by using One way Anova test followed by LSD, indicated that the number of pancreatic β-cells was significantly decreased among the groups (P < 0.05). Conclusion: Acute overtraining may cause damages of pancreatic β cells.Keywords: excessive physical work, pancreatic β cellsAbstrak: Aktivitas fisik berlebihan dapat menyebabkan terjadinya stres oksidatif yang ditandai dengan meningkatnya radikal bebas di dalam tubuh. Stres oksidatif akan menyebabkan kerusakan berbagai macam sel, salah satunya sel β pulau langerhans pankreas. Penelitian ini bertujuan untuk mengetahui aktivitas fisik maksimal akut (acute overtraining) dalam meningkatkan risiko terjadinya diabetes melitus tipe I melalui induksi kerusakan sel β pankreas. Rancangan penelitian ini ialah completely randomized design dengan sampel 36 ekor mencit yang dibagi menjadi 3 kelompok. Kelompok P0 ialah kelompok mencit tanpa perlakuan; kelompok P1 diberi perlakuan beban kerja maksimal dengan periode pemulihan selama 48 jam; dan kelompok P2 diberi perlakuan beban kerja maksimal dengan periode pemulihan selama 24 jam. Setelah 14 hari perlakuan, keseluruhan kelompok sampel diambil organ pankreasnya untuk dibuat sediaan histologik dengan pewarnaan khusus Gomori chrome hematoxylin phloxine B. Analisis deskriptif kualitatif menunjukkan bahwa morfologi jaringan pankreas pada kelompok P1 dan P2 mengalami kerusakan jika dibandingkan P0. Hasil pengamatan kuantitatif dianalisis dengan menggunakan uji One way Anova dilanjutkan dengan Least Significant Difference (LSD), menunjukkan bahwa terjadi penurunan jumlah sel β pankreas yang signifikan antar kelompok P0, P1, dan P2 (P < 0,05). Simpulan: Aktivitas fisik maksimal akut dapat menyebabkan kerusakan sel β pankreas.Kata kunci: beban kerja maksimal, sel β pankreas

scholarly journals KOMPARASI MAKRO EKONOMI NEGARA-NEGARA ASEAN TAHUN 2015-2018

2021 ◽  
Vol 2 (1) ◽  
pp. 57
Author(s):  
Allenly Aldo Likuayang ◽  
Ernest Cornelius Matindas
Keyword(s):  
Brunei Darussalam ◽  
Honestly Significant Difference ◽  
Significant Difference ◽  
Tukey Honestly Significant Difference ◽  
Post Hoc ◽  
One Way Anova

Ekonomi global yang semakin kompetitif membuat setiap negara bekerja keras untuk meningkatkan kinerja ekonomi. Penelitian ini bertujuan untuk mengetahui perbandingan kondisi makro ekonomi negara-negara ASEAN yaitu Indonesia, Brunei Darussalam, Filipina, Kamboja, Laos, Malaysia, Myanmar, Singapura, Thailand dan Vietnam selama tahun 2015 - 2018 yaitu di masa periode pemerintahan Presiden RI Ir. H. Joko Widodo. Kondisi makro ekonomi setiap negara ASEAN digambarkan melalui beberapa indikator yaitu pertumbuhan ekonomi, pendapatan per kapita, tingkat inflasi, dan tingkat pengangguran.  Perbandingan makro ekonomi pada negara ASEAN dilakukan melalui analisis komparasi uji beda One Way ANOVA untuk menjawab apakah terdapat perbedaan yang signifikan antara indikator makro ekonomi Indonesia dan 9 negara ASEAN, dan teknik uji lanjut (post hoc test) menggunakan uji Tukey Honestly Significant Difference untuk menjelaskan pola perbedaan dalam hasil uji One Way ANOVA. Hasil uji beda One Way ANOVA menununjukan pertumbuhan ekonomi, pendapatan per kapita, tingkat inflasi dan tingkat pengangguran di Indonesia dan 9 negara ASEAN masing-masing memiliki nilai signifikansi α kurang dari 0.05 sehingga disimpulkan bahwa pertumbuhan ekonomi, pendapatan per kapita, tingkat inflasi dan tingkat pengangguran di Indonesia dan 9 negara ASEAN memiliki perbedaan yang signifikan. Kata kunci: ASEAN, makro ekonomi, pertumbuhan ekonomi, pendapatan per kapita, tingkat inflasi, tingkat pengangguran

scholarly journals AB0525 NATURAL HISTORY OF CRYOGLOBULINEMIA FROM 2000 TO 2018 FROM THE LABORATORY POINT OF VIEW: AN ANALYSIS OF CRYOGLOBULIN CHARACTERISTICS IN A SINGLE CENTER.

2020 ◽  
Vol 79 (Suppl 1) ◽  
pp. 1559.3-1560
Author(s):  
G. Sandri ◽  
A. Spinella ◽  
P. Natali ◽  
D. Debbia ◽  
D. Campioli ◽  
...  
Keyword(s):  
Big Data ◽  
Chronic Hepatitis ◽  
Rare Condition ◽  
Relevant Information ◽  
Strategic Decision ◽  
Type I ◽  
Type Ii ◽  
Type Iii ◽  
Significant Difference

Background:Big data refers to large amounts of information. With today’s ever-improving technologies created by the automation and digitization, it becomes easier to convert data into relevant information, which can be used to provide better patient management, especially when it occurs a rare condition such as cryoglobulinemia (CRG).CRG is due to an immunoglobulins (Ig) that precipitate at low temperatures. There are 3 types of CRG: type I: monoclonal Ig; type II: monoclonal Ig + polyclonal Ig; type III: 2 polyclonal Ig.Objectives:The aim of this study was to analyse the available data coming to the Department of Laboratory Medicine of Modena to describe the population of patients suffering of CRG.Methods:Data from the Modena Labs network were extracted by means of the software “Pagoda”, for statistical purposes, directly connected to the Laboratories Information System (LIS). Considered time period 2000-2018Results:The analysed samples were 28,847, 4901 (17%) of which positive to the cryoglobulins (CR) detection. The typing positive CR were 4190 (85%): type 1 7.8 %, type II 48.54% and type III 43.8% The positive samples belonged to 2528 patients. Patients were 1563 (62%) women, average age 66±16, and 965 (38%) men, average age 62±16. (female/male ratio = 1.62) No statistically significant difference regarding sex and age between the 3 types. The cryoglobulinemia phenomenon is quite complex and the typing of monoclonal, polyclonal components of the Ig classes and chains has made it possible to identify 41 possible combinationsOut of 115 patients with Type 1 cryoglobulin, 73% had a monoclonal IgM and 36% monoclonal IgG; 11.2% both monoclonal IgG and IgM. The к light chain was the most frequent: 55,6% IgM-k and 23,4% IgG-k vs 29% IgM-λ and 18.2% IgG-λ. Two patients had an IgA-k cryoglobulin.Patients with Type 2 cryo were 781: monoclonal IgM-k 587 (75.1 %), 126 IgM-λ (16.1 %), 52 IgG-k (6.6 %), IgG-λ (5%), 1 IgA-λ; 2.8% had both IgG and IgM. Out of 1204 patients with Type 3 cryo, 74.8% had both polyclonal IgG and IgM, 13,8% had isolated IgM or IgG (9%); 4.8 % IgG-IgA-IgM together, both IgG and IgA in only 0,5% of patients. Almost the 50% (1255) of the patients had a diagnosis supporting the request of CR:HCV, 604 (48%); chronic hepatitis, 177 (14%); rheumatologic disorder, 107 (9%); autoimmune diseases, 83 (7%), nephropathy, 80 (7%); others, 204 (15%).Genotyped HCV+ patients were 290 (48%), with the following results: 1b, 136 (47%); 2a/2c, 65 (22%); 3a, 26 (9%); others 63 (22%).Conclusion:The data show that CRG is a pathology which affects the elderly and especially women, as in autoimmune and rheumatological diseases. The predominant CRG type is the III and the most frequently related pathology to the request for CR is HCV+ and chronic hepatitis. The most common HCV genotype are 1b and 2a/2c The cryoglobulinemia phenomenon is complex and laboratory typing is intriguing.Starting from this preliminary descriptive study, it will be possible, with the available data, to perform elaborations that allow to relate CRG with the diagnosis and verify the effect of therapy.Big Data methods’ application represents an undeniable resource for public institutions when carrying out strategic decision-making processes.Disclosure of Interests:None declared

Concept complexity W. Weawer differ from the modern scientists

2015 ◽  
Vol 4 (1) ◽  
pp. 13-22
Author(s):  
Журавлева ◽  
A. Zhuravleva ◽  
Еськов ◽  
V. Eskov ◽  
Гудкова ◽  
...  
Keyword(s):  
Social Relations ◽  
Modern Science ◽  
Self Organization ◽  
Stochastic Methods ◽  
Type I ◽  
The Third ◽  
Basic Model ◽  
Significant Difference

The present paper shows that the term “complexity” includes absolutely different notions than now it seems to be presented in modern science and philosophy. Postnon-classics has come to this new recognition too close, but, actually, it is a new recognition of uncertainty for systems of the third type (not deterministic and not stochastic). We introduce the interpretation of a type I uncertainty that implies that stochastic methods show systems identified, but methods of the theory of chaos and self-organization and neurocomputing show significant difference of target systems (processes). With specific examples presented uncertainty of type 1 and gives an idea of the uncertainty of type 2, when the distribution function f (x) for different samples are the same. At the same neuro-computers not only divides the sample, but also shows the order parameters. In this case, at the same time solve the problem of system synthesis, which in society is now very difficult to solve (the basic model of social relations now – it&#180;s deterministic society).

scholarly journals Effect of Interval Time between Corrosive and Abrasive Challenges on a Nanoparticulate Composite Resin

2021 ◽  
Author(s):  
Ana Laura Rezende Vilela ◽  
Alexandre Coelho Machado ◽  
Lucas Lemes Queiroz ◽  
Pedro Henrique Mauro Batista ◽  
André Luís Faria-e-Silva ◽  
...  
Keyword(s):  
Composite Resin ◽  
Resin Composite ◽  
Analysis Data ◽  
Control Group ◽  
Interval Time ◽  
Interval Group ◽  
Honestly Significant Difference ◽  
Significant Difference ◽  
And Control ◽  
One Way Anova

Abstract Objective This study aimed to evaluate the effect of interval time after acidic beverage intake and brushing on roughness and hardness of resin composite. Materials and Methods Nanofilled resin composites were tested as per interval time (no interval, 15 or 30 minutes) between aging media (isotonic, sports drink) and brushing. Specimens (n = 9) were subjected to three cycles daily for 5 days with immersion in beverage followed by simulated brushing (585 strokes). The brushing (control) group was submitted only in brushing cycles. Roughness and microhardness were analyzed in the baseline and end of the experiment. Surface morphology was analyzed by using scanning electron microscopy (SEM). Statistical analysis Data were analyzed by one-way ANOVA and Tukey’s honestly significant difference (α = 0.05). Results Roughness was higher in no interval group and lower in 30 minutes and control. The 15 minutes present no statistical difference between control, 30 minutes and no interval. The hardness not present difference between groups. The SEM showed the no interval more roughness than 15 and 30 minutes, control and baseline. Conclusion The interval time between erosive and abrasive challenge is important to preserve the smoothness surface of composite resin.

Sign in / Sign up

Export Citation Format

Share Document