scholarly journals Allium-Based Phytobiotic Enhances Egg Production in Laying Hens through Microbial Composition Changes in Ileum and Cecum

Animals ◽  
2021 ◽  
Vol 11 (2) ◽  
pp. 448
Author(s):  
Miguel Rabelo-Ruiz ◽  
Juan José Ariza-Romero ◽  
María Jesús Zurita-González ◽  
Antonio Manuel Martín-Platero ◽  
Alberto Baños ◽  
...  
Keyword(s):  
Relative Abundance ◽  
Egg Production ◽  
High Throughput Sequencing ◽  
Laying Hens ◽  
Microbial Composition ◽  
Positive Effects ◽  
Rare Biosphere ◽  
Food Digestibility ◽  
Unweighted Unifrac ◽  
Experimental Group

Phytobiotics (bioactive compounds extracted from plants) are one of the explored alternatives to antibiotics in poultry and livestock due to their antimicrobial activity and its positive effects on gut microbiota and productive properties. In this study, we supplemented a product based on garlic and onion compounds in the diet to laying hens at the beginning of their productive life (from 16 to 20 weeks post-hatching). The experimental group showed a significant increase in the number of eggs laid and in their size, produced in one month compared to the control. This increase in production was accompanied by microbiota changes in the ileum and cecum by means of high throughput sequencing analyses. These bacterial shifts in the ileum were mainly the result of compositional changes in the rare biosphere (unweighted UniFrac), while in the cecum, treatment affected both majority and minority bacterial groups (weighted and unweighted UniFrac). These changes in the microbiota suggest an improvement in food digestibility. The relative abundance of Lactococcus in the ileum and Lactobacillus in the cecum increased significantly in the experimental group. The relative abundance of these bacterial genera are known to have positive effects on the hosts. These results are very promising for the use of these compounds in poultry for short periods.

scholarly journals The Influence of the Mineral-Microbial Preparation on Ammonia Concentration and Productivity in Laying Hens Houses

Atmosphere ◽  
2019 ◽  
Vol 10 (12) ◽  
pp. 751 ◽  
Author(s):  
Remigiusz Gałęcki ◽  
Michał Dąbrowski ◽  
Tadeusz Bakuła ◽  
Kazimierz Obremski ◽  
Adriana Nowak ◽  
...  
Keyword(s):  
Body Weight ◽  
Linear Correlation ◽  
Egg Production ◽  
Laying Hens ◽  
Animal Health ◽  
The Body ◽  
Control Group ◽  
Microbial Composition ◽  
Experimental Group ◽  
Campylobacter Spp

The aim of this study was to evaluate the influence of the biopreparation Deodoric® on ammonia (NH3) concentration, performance, and hygiene standards in laying hen (ROSS-308) production. Statistically significant differences in NH3 concentration and the body weight of laying hens were observed between the control group (C) and the experimental group (E) where Deodoric® was applied at the set dose. In the control group, an increase in NH3 concentration could have contributed to the decrease in the body weight of laying hens, egg production, and % hen day egg production, whereas no such correlations were observed in the experimental group. A moderate linear correlation between NH3 concentration vs. humidity (r = 0.68), air flow (r = 0.48) and weakly linear correlation between NH3 concentration and age of birds (r = 0.27) was noted in group C. In group E, NH3 concentration vs. temperature (r = 0.27) and humidity (r = 0.14) were weakly correlated. Statistical analysis of changes in the microbial counts isolated from manure revealed a significant decrease of mesophilic microorganisms on day 28 decrease of Campylobacter spp. days 14 and 84 in group E. However, for the entire experimental model no statistically significant changes in the number of Campylobacter spp. and mesophilic bacteria were found. The tested preparation did not cause changes in the microbial composition of tissue swabs. Deodoric® contributes to animal welfare by reducing the ammonia concentrations in poultry houses. It is also recommended for use in poultry farms to improve animal health and performance and to generate benefits for producers.

scholarly journals Effect of Boulardii Yeast Wall Polysaccharides on Intestinal Microflora in Jejunum, Cecum and Colon for Early-Weaned Lambs by 16S rRNA Sequence Analysis

2021 ◽  
Vol 26 (01) ◽  
pp. 156-168
Author(s):  
Mengjian Liu
Keyword(s):  
Relative Abundance ◽  
Intestinal Microflora ◽  
High Throughput Sequencing ◽  
Basal Diet ◽  
Microbial Flora ◽  
Control Group ◽  
Sequencing Analysis ◽  
Microbial Composition ◽  
Intestinal Microbial Flora ◽  
Experimental Group

This study was conducted to investigate the effects of Boulardiiyeast wall polysaccharide (BRYP) on the intestinal micro-ecosystem of early-weaned lambs. A total of 60 early-weaned lambs (35-days-old, Kazak♂*Altay♀*Suffolk♂) were randomly assigned into two treatments: a control group with a basal diet, an experimental group with a basal diet and added 0.05% BRYP. The HiSeq high-throughput sequencing analysis of 16SrRNA was used to investigate the differences in intestinal microbial flora composition, diversity, relative abundance, principle coordination analysis, and the correlation between intestinal microbial flora composition and immune indices. After feeding for 40days, the addition of 0.5% BRYP in milk replacer significantly enhanced the species richness in the cecum and colon, but decreased the diversity of species in the colon (P <0.05); Compared with the control group, the relative abundance of Bacteroidetes in the experimental group was significantly enhanced, but the Proteobacteria was significantly decreased in all tested intestinal segments (P <0.05).In the jejunum, the relative abundance of Lactobacillus, Prevotella, and Fibrobacter of the experimental group were significantly enhanced than that of the control group, but the Ruminobacter was significantly decreased (P <0.05); In the cecum, the relative abundance of Bacteroides, Lactobacillus, Oscillospira and Bifidobacterium of the experimental group were significantly enhanced than that of the control group, but the Blautia were significantly decreased (P <0.05); In the colon, the relative abundance of Akkermansia, Bifidobacterium, Lactobacillus and Faecalibacteriumof the experimental group were significantly enhanced than that of the control group, but the Prevotella, Streptococcus, and Escherichia were significantly decreased (P <0.05). There were significant correlations between intestinal immune indices (IL-6, IL-10, TNF-α) and intestinal microbial composition in the colon (P <0.05). These results indicated that BRYP may contribute to the promotion of the proportion of helpful microbial populations and enhancing the balance of intestinal; Besides, BRYP may indirectly improve the intestinal immune function by changes of intestinal microflora composition, but suppress the inflammatory response in the bottom of intestinal mucosa of early-weaned lambs.© 2021 Friends Science Publishers

Association between Single Nucleotide Polymorphisms of Atp2B1 Gene and Bone Parameters of Laying Hens

2018 ◽  
Vol 11 (3) ◽  
pp. 178-182
Author(s):  
Eliska Horecka ◽  
Cenek Horecky ◽  
Lenka Kovarikova ◽  
Anna Musilova ◽  
Ales Knoll ◽  
...  
Keyword(s):  
Single Nucleotide Polymorphisms ◽  
Egg Production ◽  
Laying Hens ◽  
Compact Bone ◽  
Chromosome 1 ◽  
Nucleotide Polymorphisms ◽  
Bone Thickness ◽  
Single Nucleotide ◽  
Experimental Group ◽  
Exon 10

Experiments were performed in 110 ISA Brown egg production hens (Gallus gallus), kept from 15 to 26 weeks of age in enriched (furnished) housing technology. The present objective was to investigate the presence of single nucleotide polymorphisms (SNPs) of the ATP2B1 gene and their effects on calcium homeostasis in laying hens. The plasma membrane calcium-transporting ATPase 1 gene (ATP2B1) in hens is located on chromosome 1, region 43 273 706 – 43 305 815 bp. The ATP2B1 gene has 21 exons, and in this study three were genotyped. In each experimental group of animals, only alleles without deletions in exon 10 and only allele A in exon 12 were found. In exon 8, only genotypes CC/CC, TT/CC and TT/TT were found. These genotypes are associated with femur breaking strength, bone diameter, bone marrow diameter and compact bone thickness. No significant effects of SNPs in exon 8 on bone characteristics were found.

scholarly journals Laying performance and eggshell quality in laying hens fed diets supplemented with prebiotics and organic acids

2010 ◽  
Vol 55 (No. 7) ◽  
pp. 294-306 ◽  
Author(s):  
S. Świątkiewicz ◽  
J. Koreleski ◽  
A. Arczewska
Keyword(s):  
Organic Acids ◽  
Egg Production ◽  
Volatile Fatty Acids ◽  
Laying Hens ◽  
Feed Additives ◽  
Egg Mass ◽  
Positive Effects ◽  
Eggshell Quality ◽  
Laying Performance ◽  
Calcium And Phosphorus

The aim of the study was to evaluate the effect of organic acids and the prebiotic fructans on egg production and eggshell quality when added to the layer diet with different levels of calcium and phosphorus. The experiment was carried out on 168 Bovans Brown hens, allocated to 14 groups of 12 replications. Each hen (replication) was kept in an individual cage 40 cm &times; 40 cm in size. A 2 &times; 7 factorial arrangement, with two dietary levels of calcium and phosphorus (normal &ndash; 3.70% Ca, 0.65% P, and reduced &ndash; 3.25% Ca, 0.60% P) and with diets supplemented by selected additives (none, 0.75% inulin, 0.75% oligofructose, 0.50% volatile fatty acids (VFA), 0.25% medium chain fatty acid (MCFA), 0.30% VFA + 0.20% MCFA, 0.75% inulin + 0.50% VFA) was used. The experiment was carried out over 34 weeks, from the age of 26 to 70 weeks. There were no statistically confirmed effects of the factors studied in this experiment on egg performance, i.e. laying rate, egg mass, feed intake and feed conversion. Reducing the dietary levels of Ca and P significantly decreased eggshell percent, thickness, density and breaking strength. The additives used had a considerable effect on eggshell quality at 46, 58 and 70 weeks of age, and these positive effects were most pronounced in the case of inulin and MCFA. There was no significant interaction between Ca and P dietary levels and the additives used. It was thus concluded that selected feed additives which lower the pH of the diet and intestinal content can beneficially influence eggshell quality in older high-producing laying hens. &nbsp;

scholarly journals Dysbiosis of gut microbiota and its correlation with dysregulation of cytokines in psoriasis patients

2021 ◽  
Author(s):  
Xinyue Zhang ◽  
Kun Guo ◽  
Linjing Shi ◽  
Ting Sun ◽  
Songmei Geng
Keyword(s):  
Gut Microbiota ◽  
Relative Abundance ◽  
Gut Microbiome ◽  
High Throughput Sequencing ◽  
Interleukin 2 ◽  
Negative Relationship ◽  
Healthy Individuals ◽  
Microbial Composition ◽  
Microbiome Composition ◽  
The Relationship

Abstract Background: Psoriasis is an inflammatory skin disease associated with multiple comorbidities and substantially diminishes patients’ quality of life. The gut microbiome has become a hot topic in psoriasis as it has been shown to affect both allergy and autoimmunity diseases in recent studies. Our objective was to identify differences in the fecal microbial composition of patients with psoriasis compared with healthy individuals to unravel the microbiota profiling in this autoimmune disease.Results: We collected fecal samples from 30 psoriasis patients and 30 healthy controls, sequenced them by 16S rRNA high-throughput sequencing, and identified the gut microbial composition using bioinformatic analyses including Quantitative Insights into Microbial Ecology (QIIME) and Phylogenetic Investigation of Communities by Reconstruction of Unobserved States (PICRUSt). Our results showed that different relative abundance of certain bacterial taxa between psoriasis patients and healthy individuals, including Faecalibacterium and Megamonas, were increased in patients with psoriasis. It’s also implicated that many cytokines act as main effect molecules in the pathology of psoriasis. We selected the inflammation-related indicators that were abnormal in psoriasis patients and found the microbiome variations were associated with the level of them, especially interleukin-2 receptor showed a positive relationship with Phascolarctobacterium and a negative relationship with the dialister. The relative abundance of Phascolarctobacterium and dialister can be regard as predictors of psoriasis activity. The correlation analysis based on microbiota and Inflammation-related indicators showed that microbiota dysbiosis might induce an abnormal immune response in psoriasis. Conclusions: We concluded that the gut microbiome composition in psoriasis patients has been altered markedly and provides evidence to understand the relationship between gut microbiota and psoriasis. More mechanistic experiments are needed to determine whether the differences observed in gut microbiota are the cause or consequences of psoriasis and whether the relationship between gut microbiota and cytokines was involved.

scholarly journals Microbiome Analysis Exploring Taxonomic Diversity in Kasaragod Dwarf and Holstein Crossbred Cattle

2021 ◽  
Author(s):  
Deepthi M ◽  
Kumar Arvind ◽  
Rituja Saxena ◽  
Joby Pulikkan ◽  
Vineet K Sharma ◽  
...  
Keyword(s):  
High Throughput Sequencing ◽  
Marker Gene ◽  
Taxonomic Diversity ◽  
Crossbred Cattle ◽  
Microbial Composition ◽  
Indigenous Cattle ◽  
Animal Products ◽  
Unifrac Distance ◽  
Unweighted Unifrac ◽  
Principle Coordinate Analysis

Abstract The indigenous cattle are efficient in converting low quality feeds and forage into animal products. Kasaragod Dwarf cattle, a unique non-descriptive native cattle of Kerala, India, are noted for their unique qualities, such as low feed intake, thermotolerance, greater resistance to diseases and A2 allelic variant milk. However, owing to the higher milk yield, Holstein crossbred cattle are given more importance over Kasaragod Dwarf. The hindgut microbiota plays a major role in various biological processes such as the digestion, vitamins synthesis, and immunity in cattle. In this study, we compared the hindgut microbiota of the Kasaragod Dwarf with the highly found, Holstein crossbred utilizing 16S rRNA high-throughput sequencing for a better understanding of the relationship between the host and microbial community. Four replicates of each 20 samples comprising two cattle type (n=10) were sequenced and analyzed. Marker gene-based taxonomic analysis affirmed variations in their microbial composition. Principle Coordinate Analysis (PCoA) using weighted and unweighted UniFrac distance matrices showed the distinct microbial architecture of the two cattle type. Random Forest analysis further confirmed the distinctness and revealed the signature taxa in K-Dwarf. The study observed the predominance of feed efficiency associated genera viz., Anaerovibrio, Succinivibrio, Roseburia, Coprococcus, Anaerostipes, Paludibacter, Elusimicrobium, Sutterella, Oribacterium, Coprobacillus, and Ruminobacter in Kasaragod Dwarf cattle. The study highlights the abundance of unique and beneficial hindgut microflora found in Kasaragod Dwarf, which may attest its importance over exotic cattle breeds viz., Holstein. To our knowledge, this is the first report of Kasaragod Dwarf cattle gut microbiome profiling. This study is pivotal towards developing genetic resources for the microbial population in K-Dwarf and how it could be differentiated from Holstein crossbred cattle.

scholarly journals Increased Hatchability of Chickens against the Background of the Use of Water-Soluble Antioxidants

2021 ◽  
pp. 493-500
Author(s):  
Konstantin S. Ostrenko ◽  
Alexander A. Deltsov ◽  
Vladimir I. Maximov ◽  
Elena V. Sukharenko
Keyword(s):  
Egg Production ◽  
Laying Hens ◽  
Low Density Lipoprotein ◽  
Effective Means ◽  
Density Lipoprotein ◽  
Egg Yolk ◽  
Water Soluble ◽  
Lipoprotein Fraction ◽  
Axillary Vein ◽  
Experimental Group

The use of antioxidants is an effective means of increasing egg production and hatchability of chickens. The difficulty in application is in the methods of administration of drugs to chickens. Fat-soluble antioxidants are mainly available on the market. Aims: The aim of the study was to study the effectiveness of water-soluble antioxidants on physiological and zootechnical indicators of egg incubation and hatchability of offspring. Methodology: The study was conducted on two groups of laying hens of Ostad, selected by random sampling of one hundred heads per group. For 41 days, the chickens of the experimental group received a basic diet enriched with dihydroetoxychine (DHE) in order to increase the antioxidant status at a dosage of 100 mg/kg of feed. Samples were taken from the axillary vein on the 25th day of application of the supplement (n=5) for physiological and biochemical studies. Results: During the study, it was found that in the experimental group, the concentration of cholesterol in the high-density lipoprotein fraction doubled (P<0.01), and in the low-density lipoprotein fraction decreased by almost 50% (P<0.01) compared to the control. The concentration of malondialdehyde (MDA) in the blood of chickens of the experimental group was 82.00% relative to that in the control, and in the egg yolk – only 37.42%. Egg production of chickens of the experimental group exceeded the control by 7.27%. Conclusion: The totality of the information provided confirms the physiological adequacy for laying hens of the introduction of dihydroethoxychine in the specified dose.

scholarly journals Effects of feed supplementation with manganese from its different sources on performance and egg parameters of laying hens

2014 ◽  
Vol 59 (No. 4) ◽  
pp. 147-155 ◽  
Author(s):  
K. Venglovská ◽  
Ľ. Grešáková ◽  
I. Plachá ◽  
M. Ryzner ◽  
K. Čobanová
Keyword(s):  
Egg Production ◽  
Laying Hens ◽  
Egg Quality ◽  
Protein Hydrolysate ◽  
Basal Diet ◽  
Egg Yolk ◽  
Control Group ◽  
Feed Supplementation ◽  
Positive Effects ◽  
Dietary Treatments

The objective of this study was to compare the effects of feed supplementation of laying hens with manganese from its inorganic and organic sources on performance and some parameters of egg quality. Ninety-six hens at 20 weeks of age were randomly allocated to 4 dietary treatments, each consisting of 6 replicates (4 birds per replicate). The control group was fed unsupplemented basal diet (BD) with only natural background Mn level of 46.4 mg/kg feed. For the three experimental treatments, the BD was supplemented with 120 mg Mn/kg either from Mn-sulphate or Mn-chelate of protein hydrolysate (Mn-Pro) or Mn-chelate of glycine hydrate (Mn-Gly). After 8 weeks of dietary treatments the egg production, egg weight, feed intake, and feed efficiency were not affected by dietary treatments. Regardless of the sources, Mn supplementation to feed resulted in significantly decreased percentages of cracked eggs compared to the unsupplemented control group. The thickness, weight, proportion, and index of eggshell were significantly elevated in all groups supplemented with Mn. The intake of Mn-Gly resulted in considerably increased Mn deposition in egg yolk compared to the control eggs. In the control and Mn-sulphate groups yolk malondialdehyde (MDA) started to increase after 20 and 30 days of egg storage respectively, whereas in eggs from hens given organic Mn-sources this parameter was not affected up to 40 days. Although there were no significant differences in MDA values between the treatments until 20 days of storage, the Mn-sulphate group showed significantly higher MDA concentration in yolks compared to the control group after 30 days of storage. These results demonstrate that supplementation of hens&rsquo; diet with Mn has positive effects on eggshell quality. Feed supplementation with Mn from organic sources appears to be more effective in preventing yolk lipid oxidation during cold storage of eggs than that from Mn-sulphate. &nbsp;

Influence of enzyme feed additive on the quality of eggs on the example of PJSC “Poultry plant Chelyabinskaya”

2020 ◽  
pp. 52-59
Author(s):  
S. Ermolov ◽  
V. Kosilov ◽  
Yu. Barabanova
Keyword(s):  
Egg Production ◽  
Laying Hens ◽  
Labour Productivity ◽  
Feed Additives ◽  
Poultry Meat ◽  
Feed Additive ◽  
Simultaneous Increase ◽  
Control Group ◽  
Experimental Group

Poultry farming is one of the major branches of animal husbandry, which develops through concentration, specialization and intensification on the basis of poultry plants and poultry farms. The increase in production of eggs and poultry meat is based on a significant increase in the productivity of poultry with a simultaneous increase in its population with a high payment of feed by production and the increase in labour productivity. The purpose of the work was to study the influence of enzyme feed additives in the diets of laying hens on the quality of eggs. The researches have been carried out in PJSC “Poultry plant Chelyabinskaya”, which produces high-quality chicken eggs obtained from the poultry cross “Loman LSL-classic”. The object of study was enzyme drugs produced by Sunzyme – a multi-enzyme drug that includes xylanase, cellulase and mannase activities. The dosage of enzyme drugs in the feed mixture of laying hens was: Sunfyze 5000 – 80 g/t, Sunzyme – 100 g/t. The results of researches show that in the groups that received enzyme drugs in their diet, egg production was higher than in the control group. So, for the entire period of researches the hens of the experimental groups were significantly superior to the control group in terms of egg production per average laying hen. The largest difference was in hens of the 1st experimental group (enzyme drug Sunfyze) – 10,8 eggs; the smallest in hens of the 3rd group (enzyme drugs Sunfyze and Sunzyme) – 5,7 eggs. In hens of the 2nd experimental group compared with hens of the control group egg production was higher by 7,9 eggs. The best indicators of production efficiency have been obtained in the 1st experimental group of laying hens, which were fed with the enzyme feed additive Sunfyze to reduce the nutritional value of the diet according to the matrix.

The effectiveness of the inclusion of domestic enzymes in the compound feed to increase the egg production of laying hens and the quality of eggs

2021 ◽  
pp. 33-41
Author(s):  
L. R. Mikhailova ◽  
L. V. Zhestyanova ◽  
A. Yu. Lavrentiev ◽  
N. M. Kostomakhin ◽  
V. S. Sherne
Keyword(s):  
Production Technology ◽  
Egg Production ◽  
Laying Hens ◽  
Feed Consumption ◽  
Control Group ◽  
Egg Weight ◽  
Chicken Eggs ◽  
Experimental Group ◽  
Poultry Farming

Compound feed are subject to requirements for their composition, since they use several types of grains that contain anti-nutritive substances that reduce the digestibility and use of nutrients. One of the tasks of domestic poultry farming is to reduce losses by increasing the digestibility and accessibility of feed. Among the most effective ways to solve this problem is to add enzymes to the feed of poultry. The inclusion of enzyme drugs in the composition of compound feed contributes to the inactivation of anti-nutritive substances, which contributes to an increase in the coeffi cient of digestibility of nutrients. The purpose of the work was to establish the feasibility and eff ectiveness of the enrichment of compound feed used in the production technology of chicken eggs with mixtures of enzyme drugs of domestic production of Amylosubtilin G3x, Cellolux-F and Protosubtilin G3x. Three groups of laying hens with 57 heads in each have been formed: a control group and two experimental groups. The duration of the experiment was 420 days. Laying hens of the control group have been received farm compound feed depending on their age. Laying hens of the 1st experimental group have been received compound feed of the control group enriched with the enzymes Amylosubtilin G3x and Cellolux-F depending on the age. In the compound feed of laying hens of the 2nd experimental group enzyme drugs were additionally introduced depending on the age Amilosubtilin G3x and Protosubtilin G3x. Higher egg production rates have been observed in the 1st experimental group – 357,81 eggs, which was 6,48 % higher than in the control group and 1,84 % higher than in the 2nd experimental group. The weight of eggs of laying hens of the 1st experimental group was 1,85 g or 2,97 % more, and the 2nd experimental group was 0,7 g or 1,12 % more than in the control group. The diff erence between the egg weight in laying hens of the 1st and 2nd experimental groups was 1,85 % or 1,15 g. The lowest feed consumption of 1,37 kg for the production of 10 eggs was in the laying hens of the 1st experimental group, which was less than the indicator of the control group by 10,94 and by 4,2 % than in the 2nd experimental group.

Sign in / Sign up

Export Citation Format

Share Document