scholarly journals Alleviating Effects of Vitamins C and E Supplementation on Oxidative Stress, Hematobiochemical and Histopathological Alterations Caused by Copper Toxicity in Broiler Chickens

Animals ◽  
2021 ◽  
Vol 11 (6) ◽  
pp. 1739
Author(s):  
Mohamed A. Hashem ◽  
Sahar S. Abd El Hamied ◽  
Eman M. A. Ahmed ◽  
Shimaa A. Amer ◽  
Aziza M. Hassan
Keyword(s):  
Oxidative Stress ◽  
Vitamin E ◽  
Vitamin C ◽  
Broiler Chickens ◽  
Copper Toxicity ◽  
Basal Diet ◽  
High Serum ◽  
Control Group ◽  
Broiler Chicks ◽  
High Doses

The current investigation evaluated the alleviating effects of vitamin C and vitamin E on oxidative stress, hematobiochemical, and histopathological changes in the kidney induced by copper sulfate (CuSO4) toxicity in chickens. Two hundred and fifty-one-day-old male broiler chicks were randomly allotted into five experimental groups (five replicates/group, ten chicks/replicate): 1st group—basal diet with no additives (control group), 2nd group—basal diet complemented with CuSO4 (300 mg/kg diet), 3rd group—basal diet with CuSO4 (300 mg/kg diet) + vitamin C (250 mg/kg diet), 4th group—basal diet with CuSO4 (300 mg/kg diet) + vitamin E (250 mg/kg diet), and 5th group—basal diet with CuSO4 (300 mg/kg diet) + vitamin C (250 mg/kg diet) + vitamin E (250 mg/kg diet) for a 42 day feeding period. The results showed a significant reduction in red blood cells (RBCs), hemoglobin (Hb) concentration, and hematocrit values as well as total leukocyte counts (WBCs), lymphocyte, heterophil, and monocyte counts in the CuSO4-intoxicated birds (2.42 × 106/µL, 9.54 g/dL, 26.02%, 15.80 × 103/µL, 7.86 × 103/µL, 5.26 × 103/µL, and 1.18 × 103/µL, respectively, at the 6th week) compared to (2.79 × 106/µL, 10.98 g/dL, 28.46%, 21.07 × 103/µL, 10.84 × 103/µL, 7.12 × 103/µL, and 1.60 × 103/µL, respectively) in the control group. Moreover, CuSO4-intoxicated birds showed hypoglycemia with a rise in serum uric acid and creatinine levels (122.68, 5.18, and 0.78 mg/dL at the 6th week) compared to (159.46, 4.41, and 0.61 mg/dL) in the control group. The CuSO4 toxicity in birds induced oxidative stress, indicated by a high serum malondialdehyde level (MDA) and diminished activity of the antioxidant enzymes (glutathione peroxidase (GSH-Px) and superoxide dismutase (SOD)) (2.01 nmol/mL, 37.66 U/mL, and 2.91 U/mL, respectively, at the 6th week) compared to (1.34 nmol/mL, 57.00 U/mL, 4.99 U/mL, respectively) in the control group. High doses of Cu exposure caused severe microscopic alterations in kidney architecture. The addition of vitamins C and E, singularly or in combination, displayed a beneficial effect in alleviating these harmful effects of Cu toxicity. These findings showed the possible mitigating impacts of dietary antioxidants on the hematobiochemical alterations, oxidative stress, and kidney damage induced by CuSO4 toxicity.

scholarly journals Comparison of Oxidant-Antioxidant Status in Patients with Vitiligo and Healthy Population

2015 ◽  
Vol 12 (2) ◽  
pp. 132-136 ◽  
Author(s):  
S Agrawal ◽  
A Kumar ◽  
TK Dhali ◽  
SK Majhi
Keyword(s):  
Oxidative Stress ◽  
Free Radicals ◽  
Vitamin E ◽  
Vitamin C ◽  
Control Group ◽  
Healthy Population ◽  
Significant Difference ◽  
Destruction Of Melanocytes ◽  
And Control

Background Vitiligo is a well-recognized pigmentary disorder of the skin and /or mucous membrane characterized by circumscribed ivory or chalky white macules devoid of identifiable melanocytes. The pathogenesis of vitiligo is complex and still not well understood. According to autocytotoxic hypothesis, oxidative stress has been suggested to be the initial pathogenic event in melanocyte degeneration. The role of free radicals and oxidative damage in the pathophysiology of vitiligo has been documented in recent studies.Objective To evaluate the role of oxidative stress in patients with vitiligo and of healthy controls by measuring levels of the oxidant malondialdehyde (MDA) and antioxidants vitamin C and vitamin E in serum and catalase (CAT) in erythrocytes.Method A total of 80 clinically diagnosed cases of vitiligo and 80 control subjects were included in the study to assess the activity of MDA, vitamin C and vitamin E in serum and CAT in erythrocytes of patients and controls by using the spectrophotometric assay.Result There was statistically significant increase in the levels of MDA in patients with vitiligo compared to the control group (p<0.001). No significant difference was found in the levels of vitamin C (p=0.411) and vitamin E (p=0.771) between the patients with vitiligo and control group. The levels of CAT in the vitiligo patients were found to be significantly lower than those of controls (p<0.001).Conclusion Increased oxidative stress and decreased catalase have been observed in vitiligo patients and the data suggesting that the free radicals may be involved in the destruction of melanocytes or dysregulation of melanogenesis.Kathmandu University Medical Journal Vol.12(2) 2014: 132-136

scholarly journals Effect of Adding Different Levels of Portulaca oleracea L. Seeds and Leaves Powder to The Diet on Productive and Physiological Performance of Broiler Chickens (Ross 308)

2021 ◽  
Vol 34 (1) ◽  
pp. 38-48
Author(s):  
Ahmed J. Shirshaab ◽  
Jaffar M. Jassim
Keyword(s):  
Broiler Chickens ◽  
Basal Diet ◽  
Serum Glucose ◽  
Portulaca Oleracea ◽  
Control Group ◽  
Broiler Chicks ◽  
Positive Role ◽  
Randomized Design ◽  
Escherichia Coli Bacteria ◽  
Different Levels

This study was conducted in the Poultry Field of the College of Agriculture, Basrah University for the period from 20/9/2019 to 24/10/2019 to investigate the effect of adding different levels of Portulaca oleracea L. seeds and leaves powder on some physiological characteristics of broilers. A total 216 one day old unsexed Ross-308 broiler chicks were used in this study. The chicks were randomly distributed into six treatments replicated three times (12 chicks each replicate). The experiment was designed as a completely randomized design (CRD). The first treatment was control (basal diet), P. oleracea seed powder was added as 5 & 10 g.kg-1 for the 2nd and 3rd treatments. Leaves powder was added as 5 and 10 g.kg-1 to the basal diet in treatment 4th and 5th. In the 6th treatment 5 gm of seed and 5 gm of leaves powder was added to the basal diet. The results showed that there was a significant (P <0.05) decrease in serum glucose, cholesterol and triglyceride concentrations in all additive treatments compared to control. There was also a significant decrease (P <0.05) in Escherichia coli bacteria count, as well as a significant improvement (P <0.05) in the number of Lactobacilli bacteria in all addition treatments compared to the control group. The addition of P. oleracea seeds and leaves powder had no significant effect on some haematological indices and on the total protein concentration in the serum. The study confirms the importance of adding powder of seeds or leaves in the broiler diet because it has a positive role in improving some of the physiological and microbial blood characteristics of the broiler.

Adverse effects in broiler chickens fed a high lycopene concentration supplemented diet

2013 ◽  
Vol 93 (2) ◽  
pp. 231-241 ◽  
Author(s):  
Luisa Pozzo ◽  
Martina Tarantola ◽  
Elena Biasibetti ◽  
Maria Teresa Capucchio ◽  
Maddalena Pagella ◽  
...  
Keyword(s):  
Antioxidant Enzymes ◽  
Adverse Effects ◽  
Broiler Chickens ◽  
Femoral Vein ◽  
Basal Diet ◽  
Bursa Of Fabricius ◽  
Control Group ◽  
Broiler Chicks ◽  
Breast Meat ◽  
Liver And Kidney

Pozzo, L., Tarantola, M., Biasibetti, E., Capucchio, M. T., Pagella, M., Mellia, E., Bergagna, S., Gennero, M. S., Strazzullo, G. and Schiavone, A. 2013. Adverse effects in broiler chickens fed a high lycopene concentration supplemented diet. Can. J. Anim. Sci. 93: 231–241. The present investigation was designed to assess the effects of the administration of a high dietary lycopene dose to broiler chickens on growth and slaughter performances, haemato–biochemical parameters, antioxidant enzymes, histological lesions and lycopene accumulation in the tissues. Thirty-six 1-d-old male Hubbard broiler chicks were examined and divided randomly into two groups (three replicates for each dietary treatment): the Control group, which received a basal diet, and the Lycopene group, which received the basal diet supplemented with 500 mg lycopene kg−1diet. The experimental period lasted 35 d and growth performance was recorded on a weekly basis. At the end of the experiment (day 35), blood samples (n=12) were collected from the femoral vein. Erythrocyte and leukocyte numbers were assessed and the heterophil-to-lymphocyte ratio was determined. The alpha-1-acid glycoprotein, lysozyme, total protein and the electrophoretic patterns were evaluated in serum samples. Nine chickens per group were slaughtered to measure the carcass yields and the weight of the liver, spleen, bursa of Fabricius and thymus. Spleen, liver, and bursa of Fabricius samples were collected for histological examination and fixed in 10% neutral buffered formalin. The lycopene concentration, thiobarbituric acid reactive substances (TBARS) and chemical composition were assayed in breast meat and thigh samples. The TBARS, glutathione content, catalase and superoxide dismutase activity were measured in liver and kidney samples. The high lycopene concentration supplemented diet did not affect the birds’ growth, slaughter performance or antioxidant enzymes in the breast meat, thigh meat, liver and kidney samples. The concentrations of lycopene in the breast and thigh of the lycopene-supplemented group were 0.10±0.05 mg kg−1and 0.42±0.35 mg kg−1, respectively, while no lycopene was found in the Control group. Spleen and bursa of Fabricius of birds fed a diet supplemented with a high level of lycopene showed weight decrease (P<0.05) and degenerative lesions (P<0.05). Moreover, lycopene supplementation reduced the serum protein concentration, albumin, alpha and the gamma globulin serum concentration (P<0.05).

scholarly journals Effect of Dietary Lead on Intestinal Nutrient Transporters mRNA Expression in Broiler Chickens

2015 ◽  
Vol 2015 ◽  
pp. 1-8 ◽  
Author(s):  
Roohollah Ebrahimi ◽  
Mohammad Faseleh Jahromi ◽  
Juan Boo Liang ◽  
Abdoreza Soleimani Farjam ◽  
Parisa Shokryazdan ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Gene Expression ◽  
Antioxidant Enzymes ◽  
Growth Performance ◽  
Feed Efficiency ◽  
Broiler Chickens ◽  
Body Weight Gain ◽  
Basal Diet ◽  
Broiler Chicks ◽  
Nutrient Transporters

Lead- (Pb-) induced oxidative stress is known to suppress growth performance and feed efficiency in broiler chickens. In an attempt to describe the specific underlying mechanisms of such phenomenon we carried out the current study. Ninety-six one-day-old broiler chicks were randomly assigned to 2 dietary treatment groups of 6 pen replicates, namely, (i) basal diet containing no lead supplement (control) and (ii) basal diet containing 200 mg lead acetate/kg of diet. Following 3 weeks of experimental period, jejunum samples were collected to examine the changes in gene expression of several nutrient transporters, antioxidant enzymes, and heat shock protein 70 (Hsp70) using quantitative real-time PCR. The results showed that addition of lead significantly decreased feed intake, body weight gain, and feed efficiency. Moreover, with the exception of GLUT5, the expression of all sugar, peptide, and amino acid transporters was significantly downregulated in the birds under Pb induced oxidative stress. Exposure to Pb also upregulated the antioxidant enzymes gene expression together with the downregulation of glutathione S-transferase and Hsp70. In conclusion, it appears that Pb-induced oxidative stress adversely suppresses feed efficiency and growth performance in chicken and the possible underlying mechanism for such phenomenon is downregulation of major nutrient transporter genes in small intestine.

Nephrotoxicity in rats induced by chlorpryfos-ethyl and ameliorating effects of antioxidantsy

2002 ◽  
Vol 21 (4) ◽  
pp. 223-230 ◽  
Author(s):  
M Oncu ◽  
F Gultekin ◽  
E Karaöz ◽  
I Altuntas ◽  
N Delibas
Keyword(s):  
Vitamin E ◽  
Vitamin C ◽  
Mononuclear Cells ◽  
Kidney Tissue ◽  
Thiobarbituric Acid ◽  
Glomerular Sclerosis ◽  
Control Group ◽  
Thiobarbituric Acid Reactive Substance ◽  
High Doses ◽  
Chlorpyrifos Ethyl

Nephrotoxicity induced by chlorpyrifos-ethyl (CE) and ameliorating effects of melatonin and vitamin E plus vitamin C were evaluated in rats exposed to CE. Experimental groups were as follows: control (C), CE treated (CE), vitamin E plus vitamin C treated (Vit), melatonin treated (Mel), vitamin E plus vitamin C plus CE treated (Vit+CE), and melatonin plus CE treated (Mel+CE). The rats in the CE, Vit+CE and Mel+CE groups were administered orally with CE in two equal doses of 41 mg/kg body weight (0.25 LD50). Melatonin and vitamins E and C were administrated intramuscularly at the doses of 10, 150 and 200 mg/kg, respectively. The levels of thiobarbituric acid reactive substance (TBARS) and antioxidant potential (AOP), and the activities of glutathione peroxidase (GSHPx), catalase (CAT) and superoxide dismutase (SOD) were studied in the homogenates of kidney tissue. There were no significant differences in the activities of SOD and CAT between the experimental groups. The level of TBARS increased significantly (P<0.05) while AOP decreased significantly (P<0.05) in the CE group compared with the C group. GSH-Px activity was significantly (P<0.05) lower in the CE group and higher in the melatonin group than the control group. Histopathological changes were found in the kidney tissue of rats treated with CE. These were infiltration in mononuclear cells at perivascular and peritubular areas, hydropic degenerations in tubule epithelium and glomerular sclerosis. The severity of the lesions was reduced by administration of vitamins and melatonin. These results suggest that CE increases lipid peroxidation and decreases AOP by increasing oxidative stress, and that high doses of melatonin and a combination of vitamin E plus vitamin C considerably reduce the toxic effect of CE on kidney tissue of rats.

scholarly journals Study the Effect of Vitamins (C and E) on Oxidative Stress and Antioxidants Changes Induced by VCM in Male Rats

2020 ◽  
Vol 11 (03) ◽  
pp. 430-434
Author(s):  
Shaymaa J. Shamran ◽  
Haider S. Jaffat
Keyword(s):  
Oxidative Stress ◽  
Vitamin E ◽  
Vitamin C ◽  
Male Rats ◽  
Control Group ◽  
Protective Effects ◽  
Vancomycin Group ◽  
Animal House ◽  
Antioxidant Effects ◽  
Oxidative Effect

The current study was designed to determine the antioxidant effects of vitamin C and vitamin E against oxidative stress induced by vancomycin in some antioxidants changes in the male rats. The study was conducted in the animal house of the Faculty of Science/University of Kufa for the period from April, 2018 to May, 2018 on 119 animals of male rats aged 2.5–3 months and the weight of 150-200 gm. Two experiments designed in this study addressed the first and two experiments to study the oxidative effect of vancomycin in addition to the protective effects of vitamin C and vitamin E to reduce these effects in the treatment of animals for one week and three weeks with vancomycin and vancomycin plus vitamins. The results indicated a significant increase (p less than 0.05) in the MDA, CAT, and significant decrease (p less than 0.05) in SOD, and GPX. In the animals treated with vancomycin 40,60 mg/kg only compared to the control group for the two periods of administration at the same time occur a significant decrease(p less than 0.05) in the MDA, CAT and a significant increase (p less than 0.05) in the SOD and GPX after treated animals with vancomycin 40,60 mg/kg with vitamin C and vitamin E for a period of one and three weeks compared with vancomycin group.

Phytogenics (Lemon and orange peels) influenced the performance, gut morphology and blood profile of broiler chickens

2020 ◽  
Vol 47 (3) ◽  
pp. 234-244
Author(s):  
A. O. Fafiolu ◽  
J. O. Alabi ◽  
C. G. Godwin ◽  
I. D. Dada ◽  
A. A. Orimogunje ◽  
...  
Keyword(s):  
Lipid Profile ◽  
Growth Performance ◽  
Broiler Chickens ◽  
Haemoglobin Concentration ◽  
Basal Diet ◽  
Feed Additives ◽  
Control Group ◽  
Broiler Chicks ◽  
Blood Profile ◽  
Gut Morphology

This study evaluated the effect of phytogenic feed additives (Lemon peel, LP and Orange peel, OP) added singly or in combination to basal diets on the performance, gut morphology, haematology, serum biochemistry and lipid profile of broiler chickens. Four nutritionally adequate Corn-Soybean based diets were generated by the addition of LPand/or OPto basal diet. A total of 320, 1-d-old Marshall strain broiler chicks were given the basal diet supplemented with no additive (T1), 500g/tonne of LP (T2), OP (T3), or LP+OP (T4) in a study which lasted for 42 days. Growth performance was assessed weekly while gut and blood samples were collected at d 42 for examination. Data generated were analyzed using ANOVA with a P<0.05 level of significance. Results showed that chickens fed diets supplemented with LP and OP had similar (P>0.05) growth performance compared with the control group. Higher (P<0.05) duodenal and jejunal villus height were observed in chickens fed LP and OP, respectively. Phytogenics inclusion caused reduced (P<0.05) laminal propria depth at the ileum segment. Higher (P<0.05) PCV (30.00%), total protein (6.89 g/L) and albumin (3.67 g/L) were observed in chickens on LP+OP. Haemoglobin concentration (Hb) and RBC were not affected by the treatments imposed. Serum creatinine, uric acid and liver enzymes (AST and ALT) activity were reduced (P<0.05) with inclusion of LP and OP in broiler diets. Increase (P<0.05) HDL and decrease total cholesterol, LDL and triglycerides were observed in broilers fed LP and OP. Dietary inclusion of LP and OP had positive influence on gut morphology, RBC production, plasma protein reserves and lipid profile of broiler chickens.

Evaluation of turmeric extract on performance indices impressed by induced aflatoxicosis in broiler chickens

2011 ◽  
Vol 27 (10) ◽  
pp. 956-960 ◽  
Author(s):  
Nader Rangsaz ◽  
Majid Gholami Ahangaran
Keyword(s):  
Body Weight ◽  
Broiler Chickens ◽  
Body Weight Gain ◽  
Curcuma Longa ◽  
Basal Diet ◽  
Control Group ◽  
Broiler Chicks ◽  
Feed Conversion ◽  
Performance Indices ◽  
Turmeric Extract

The purpose of this study was evaluation of ethanolic turmeric extract (ETE; Curcuma longa) effect on overall performance including body weight (BW), body weight gain (BWG), feed intake and feed conversion ratio (FCR) weekly and cumulative for a period of 4 weeks with 300 commercial broiler chicks (Ross strain). These chicks were randomly divided into four groups with three replicates of 15 chicks in each replicate. In group A, chickens were fed a basal diet, in group B, chickens were fed a basal diet plus 3 ppm productive aflatoxin. In group C, chickens consumed a basal diet plus 0.05% ETE and in group D, chickens received a basal diet with 0.05% ETE plus 3 ppm productive aflatoxin. Aflatoxin production by Aspergillus parasiticus (PTTC NO:1850) in maize was according to the Shotwell method. The results revealed that there were no significant differences in BW, BWG and FCR between groups fed turmeric at 0.05% and the control group. The supplement of ETE in a diet containing 3 ppm aflatoxin can significantly improve performance indices compared with the group that consumed aflatoxin alone. In conclusion, our results suggest that turmeric extract ( Curcuma longa) can provide protection against the negative effects of aflatoxin on performance of broiler chickens.

scholarly journals Pengaruh kombinasi vitamin c dan vitamin e terhadap Kadar malondialdehid plasma pasien diabetes mellitus tipe 2

2016 ◽  
Vol 4 (1) ◽  
pp. 1-8
Author(s):  
Yade Kurnia Yasin ◽  
Martha Irene Kartasurya ◽  
RA Kisdjamiatun RMD
Keyword(s):  
Oxidative Stress ◽  
Diabetes Mellitus ◽  
Vitamin E ◽  
Treatment Group ◽  
Vitamin C ◽  
Fasting Blood Glucose ◽  
Control Group ◽  
Double Blind ◽  
Control Group Design ◽  
Before And After

Background: Malondialdehyde (MDA) levels produced by oxidative stress in type 2 diabetes mellitus (T2DM) is higher than in non diabetes patients. Vitamin C and E inhibit oxidative stress and MDA production. The purpose of this study was to prove the effects of combined vitamin C and vitamin E on MDA plasma levels in T2DM patients.Methods: This double blind randomized pre post test control group design was carried out on 35 patients with T2DM without complication (age 40-60 yr) in Makassar. The treatment group (n=18) received vitamin C 250 mg/day plus vitamin E 400 IU/day and the control group (n=17) received placebo (seaweed powder 250 mg), for six weeks. Vitamin C was consumed before meal and vitamin E after meal. MDA levels were measured before and after supplementation by using TBARs method. Food intake and activity were derived from 24-hour recall method.Results: There was no different on MDA levels at baseline (p=0.151). At the end of the study, MDA levels increased in control group (5.8±2.74 to 7.2±3.00 nmol/ml; p=0.044) but not in the treatment group (7.2±2.88 to 7.7±2.02 nmol/ml; p=0.490). There was no difference in MDA alteration between the goups (p=0.316). There was no different in MDA levels at the end of the study (p=0.061) and confirmed after controlling the confounding variables: vitamin E intake and fasting blood glucose (p=0.809). Conclusion: Combined vitamin C 250 mg and vitamin E 400 IU for 6 weeks did not reduce MDA levels, but inhibit MDA production in T2DM patients.

scholarly journals Growth Performance, Nutrient Digestibility, Biochemical Properties, Hematological Traits, and Intestinal Histopathology of Broiler Chicks Fed Mannan Oligosaccharides

2021 ◽  
Vol 11 (4) ◽  
pp. 621-633
Author(s):  
Abdallah Ali Ghazalah ◽  
Mohamed Ahmed Fouad El-Manylawi ◽  
Hady Fathy Abbas Motawe ◽  
Marwa Salah Khattab ◽  
Yara Ibrahem Youssef
Keyword(s):  
Growth Performance ◽  
Broiler Chickens ◽  
Density Lipoprotein ◽  
Basal Diet ◽  
Blood Parameters ◽  
Nutrient Digestibility ◽  
The Other ◽  
Control Group ◽  
Broiler Chicks ◽  
Treatment Groups

, , , , and Antibiotics as growth promoters in poultry diets are currently restricted, so other feed additives, such as prebiotics and probiotics, have been suggested as an antibiotics alternative to improve the performance and gut health of poultry. The current experiment was conducted to study the effects of adding Mannan oligosaccharides (MOS) as a potential replacement for an antibiotic on productive performance, nutrient digestibility, some blood parameters, and caecal microbiota of broiler chickens. For conducting the current research experiment, a total of 180 one–day old Ross broiler chicks were randomly divided into one control group fed a basal diet and four dietary treatments with six replicates for each treatment. The treatment groups were fed a basal diet supplemented with different levels of MOS 0.5, 1, and 2 g/Kg diet and Lincomycin 4.4 mg/Kg diet during 35 days of the feeding trial. With exception of the growing period, the group fed a basal diet supplemented with 2 g MOS/Kg feed had considerably higher body weight and weight gain, while having the lowest feed consumption and best feed conversion ratio compared to the other treatment groups, during all experimental periods. Moreover, dietary supplementation of MOS resulted in a significant decrease in the counts of caecal E. coli and Enterococcus, while Lactobacillus and Yeast bacteria counts were significantly higher, compared to non-supplemented groups. Broiler chicks having a 2 g MOS/kg diet recorded lower values of cholesterol, low-density lipoprotein (LDL), urea, and liver enzymes, including Aspartate transaminase (AST) and Alanine aminotransferase (ALT), while they recorded significantly higher high-density lipoprotein (HDL), compared to the other experimental groups. Group treated with MOS 2 g/Kg feed improved digestibility of crude protein, ether extract, crude fiber, nitrogen-free extract, and organic matter, compared to the control group, Additionally, MOS supplementation also increased the intestinal villi length, width, and crypt depth and decreased intestinal inflammation, compared to the control group. In conclusion, supplementation of MOS at 2 g/kg diet improved growth performance, digestibility, and blood parameters without having adverse effects on the intestine of broiler chickens, comparable to the Lincomycin.

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