scholarly journals PEG-Functionalized Magnetite Nanoparticles for Modulation of Microbial Biofilms on Voice Prosthesis

Antibiotics ◽  
2021 ◽  
Vol 11 (1) ◽  
pp. 39
Author(s):  
Mara Caciandone ◽  
Adelina-Gabriela Niculescu ◽  
Aurelian Radu Roșu ◽  
Valentina Grumezescu ◽  
Irina Negut ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Magnetite Nanoparticles ◽  
Inhibitory Effect ◽  
Voice Prosthesis ◽  
Biologically Active ◽  
Precipitation Method ◽  
Laser Evaporation ◽  
Antibiofilm Activity ◽  
Bacterial Strains ◽  
Scanning Calorimetry

This study reports the fabrication of nanostructured coatings based on magnetite, polyethyleneglycol, and biologically active molecule (polymyxin B-PM) for producing biofilm-resistant surfaces (voice prosthesis). Magnetite nanoparticles (MNPs) have been synthesized and functionalized using a co-precipitation method and were further deposited into thin coatings using the matrix-assisted pulsed laser evaporation (MAPLE) technique. The obtained nanoparticles and coatings were characterized by X-ray diffraction (XRD), thermogravimetric analysis with differential scanning calorimetry (TGA-DSC), scanning electron microscopy (SEM), transmission electron microscopy with selected area electron diffraction (TEM-SAED), Fourier-transform infrared spectroscopy (FT-IR), and infrared microscopy (IRM). Their antibiofilm activity was tested against relevant Staphylococcus aureus and Pseudomonas aeruginosa bacterial strains. The Fe3O4@PEG/PM surface of modified voice prosthesis sections reduced the number of CFU/mL up to four orders of magnitude in the case of S. aureus biofilm. A more significant inhibitory effect is noticed in the case of P. aeruginosa up to five folds. These results highlight the importance of new Fe3O4@PEG/PM in the biomedical field.

scholarly journals Biofilm-Resistant Nanocoatings Based on ZnO Nanoparticles and Linalool

Nanomaterials ◽  
2021 ◽  
Vol 11 (10) ◽  
pp. 2564
Author(s):  
Vera Alexandra Spirescu ◽  
Raluca Șuhan ◽  
Adelina-Gabriela Niculescu ◽  
Valentina Grumezescu ◽  
Irina Negut ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Zno Nanoparticles ◽  
Intraperitoneal Administration ◽  
Physicochemical Characterization ◽  
Biologically Active ◽  
Precipitation Method ◽  
In Vitro Assays ◽  
Laser Evaporation ◽  
Zno Nps

Biofilms represent an increasing challenge in the medical practice worldwide, imposing a serious threat to public health. As bacterial strains have developed antibiotic resistance, researcher’s attention has been extensively focused on developing more efficient antimicrobial strategies. In this context, the present study reports the synthesis, physicochemical characterization, ex vivo biodistribution, and in vitro evaluation of the capacity of nanostructured surfaces based on zinc oxide (ZnO) and biologically active molecules to modulate clinically relevant microbial biofilms. ZnO nanoparticles (NPs) were synthesized through a co-precipitation method without thermal treatment. The matrix-assisted pulsed laser evaporation (MAPLE) was applied for preparing nanostructured coatings based on ZnO NPs surface modified with linalool that were further characterized by X-ray diffraction (XRD), thermogravimetric analysis with differential scanning calorimetry (TGA-DSC), scanning electron microscopy (SEM), transmission electron microscopy with selected area electron diffraction (TEM-SAED), Fourier-transform infrared spectroscopy (FT-IR), and infrared microscopy (IRM). Histological analyses carried out at 7 days and 14 days after the intraperitoneal administration of linalool modified ZnO NPs revealed the absence of the latter from the brain, kidney, liver, lung, myocardium, and pancreas. Through in vitro assays on prokaryotic cells, it was proven that ZnO coatings hinder microbial biofilm formation of both Gram-positive and Gram-negative bacteria strains.

scholarly journals Obtaining and Characterizing Thin Layers of Magnesium Doped Hydroxyapatite by Dip Coating Procedure

Coatings ◽  
2020 ◽  
Vol 10 (6) ◽  
pp. 510 ◽  
Author(s):  
Daniela Predoi ◽  
Simona Liliana Iconaru ◽  
Mihai Valentin Predoi ◽  
Mikael Motelica-Heino ◽  
Nicolas Buton ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Antimicrobial Properties ◽  
Dip Coating ◽  
Thin Layers ◽  
Precipitation Method ◽  
Bacterial Strains ◽  
Antimicrobial Studies ◽  
Hydroxyapatite Coatings ◽  
Coating Procedure ◽  
Co Precipitation

A simple dip coating procedure was used to prepare the magnesium doped hydroxyapatite coatings. An adapted co-precipitation method was used in order to obtain a Ca25−xMgx(PO4)6(OH)2, 25MgHAp (xMg = 0.25) suspension for preparing the coatings. The stabilities of 25MgHAp suspensions were evaluated using ultrasound measurements, zeta potential (ZP), and dynamic light scattering (DLS). Using transmission electron microscopy (TEM) and scanning electron microscopy (SEM) information at nanometric resolution regarding the shape and distribution of the 25MgHAp particles in suspension was obtained. The surfaces of obtained layers were evaluated using SEM and atomic force microscopy (AFM) analysis. The antimicrobial evaluation of 25MgHAp suspensions and coatings on various bacterial strains and fungus were realized. The present study presents important results regarding the physico-chemical and antimicrobial studies of the magnesium doped hydroxyapatite suspensions, as well as the coatings. The studies have shown that magnesium doped hydroxyapatite suspensions prepared with xMg = 0.25 presented a good stability and relevant antimicrobial properties. The coatings made using 25MgHAp suspension were homogeneous and showed remarkable antimicrobial properties. Also, it was observed that the layer realized has antimicrobial properties very close to those of the suspension. Both samples of the 25MgHAp suspensions and coatings have very good biocompatible properties.

scholarly journals Characterization and Antibacterial Behavior of MgO-PEG Nanoparticles Synthesized via Co-Precipitation Method

2016 ◽  
Vol 70 ◽  
pp. 33-41
Author(s):  
V. Karthikeyan ◽  
S Dhanapandian ◽  
C Manoharan
Keyword(s):  
Electron Microscopy ◽  
Crystallite Size ◽  
Pathogenic Bacteria ◽  
Analytical Techniques ◽  
Precipitation Method ◽  
Diffusion Method ◽  
Average Crystalline Size ◽  
Chemical Compositions ◽  
Bacterial Strains ◽  
Size And Morphology

A surfactant assisted precipitation method is employed for the preparation of nanostructured magnesium oxide with flake-like nanoparticles. The influence of surfactant on the crystallite size and morphology of MgO was studied using various parameters. The synthesized MgO nanomaterials were characterized by using FTIR, XRD, FE-SEM and EDAX analytical techniques in order to evaluate the formation, crystalline phase morphologies, microstructures and chemical compositions. The powder X-ray power diffraction (XRD) analysis revealed the average crystalline size of 15.34 nm with cubic structure. The crystallite size increased with increasing amount of PEGs. Field Emission Scanning Electron Microscopy (FFSEM) and Transmission Electron Microscopy (TEM) showed that the surfactant strongly affect the size and morphology of nanostructure. Fourier-transform infrared spectroscopy studies indicated the formation of MgO with the characteristic vibration mode of Mg-O. Further, the antibacterial effect of MgO nanoparticles evaluated against pathogenic bacteria by agar diffusion method showed that the nanoparticles have reasonable antibacterial activity against both gram positive (S.aureus) and gram negative (E.coli) pathogenic bacterial strains and retains potential application in pharmaceutical and biomedical industries.

Formulation and Characterization of Nateglinide Nanosuspension by Precipitation Method

2014 ◽  
Vol 7 (4) ◽  
pp. 2685-2691
Author(s):  
Amruta Papdiwal ◽  
Kishor Sagar ◽  
Vishal Pande
Keyword(s):  
Particle Size ◽  
Dissolution Rate ◽  
Water Solubility ◽  
Average Particle Size ◽  
Biologically Active ◽  
In Vitro Dissolution ◽  
Precipitation Method ◽  
Average Particle ◽  
Scanning Calorimetry

Poor water solubility and slow dissolution rate are major issues for the majority of upcoming and existing biologically active pharmaceutical compounds. Nateglinide is Biopharmaceutical Classification System Class-II drug that has low solubility and high permeability. The purpose of the present study was to improve the solubility and dissolution rate of Nateglinide by the preparation of nanosuspension by the nanoprecipitation technique. Nateglinide nanosuspension was evaluated for its particle size, in vitro dissolution study, and characterized by differential scanning calorimetry and scanning electron microscopy. The optimized formulation showed an average particle size of 207 nm and zeta potential of -25.8 mV. The rate of dissolution of the optimized nanosuspension was enhanced by 83% in 50 min relative to micronized suspension of nateglinide (37% in 50 min). This improvement was mainly due to the formulation of nanosized particles of Nateglinide. Stability study revealed that nanosuspension was more stable at room temperature and refrigerator condition with no significant change in particle size distribution. These results indicate that the nateglinide loaded nanosuspension may significantly improve in vitro dissolution rate and thereby possibly enhance the onset of therapeutic effect.

scholarly journals Nanostructured Thin Coatings Containing Anthriscus sylvestris Extract with Dual Bioactivity

Molecules ◽  
2020 ◽  
Vol 25 (17) ◽  
pp. 3866
Author(s):  
Irina Negut ◽  
Valentina Grumezescu ◽  
Alexandru Mihai Grumezescu ◽  
Alexandra Cătălina Bîrcă ◽  
Alina Maria Holban ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Plant Extracts ◽  
Water Solubility ◽  
Prolonged Exposure ◽  
Composite Coatings ◽  
Microbial Colonization ◽  
Precipitation Method ◽  
In Vitro Toxicity ◽  
Laser Evaporation ◽  
Anthriscus Sylvestris

Plant extracts are highly valuable pharmaceutical complexes recognized for their biological properties, including antibacterial, antifungal, antiviral, antioxidant, anticancer, and anti-inflammatory properties. However, their use is limited by their low water solubility and physicochemical stability. In order to overcome these limitations, we aimed to develop nanostructured carriers as delivery systems for plant extracts; in particular, we selected the extract of Anthriscus sylvestris (AN) on the basis of its antimicrobial effect and antitumor activity. In this study, AN-extract-functionalized magnetite (Fe3O4@AN) nanoparticles (NPs) were prepared by the co-precipitation method. The purpose of this study was to synthesize and investigate the physicochemical and biological features of composite coatings based on Fe3O4@AN NPs obtained by matrix-assisted pulsed laser evaporation technique. In this respect, laser fluence and drop-casting studies on coatings were performed. The physical and chemical properties of laser-synthesized coatings were investigated by scanning electron microscopy, while Fourier transform infrared spectroscopy comparative analysis was used for determining the chemical structure and functional integrity. Relevant data regarding the presence of magnetic nanoparticles as the only crystalline phase and the size of nanoparticles were obtained by transmission electron microscopy. The in vitro toxicity assessment of the Fe3O4@AN showed significant cytotoxic activity against human adenocarcinoma HT-29 cells after prolonged exposure. Antimicrobial results demonstrated that Fe3O4@AN coatings inhibit microbial colonization and biofilm formation in clinically relevant bacteria species and yeasts. Such coatings are useful, natural, and multifunctional solutions for the development of tailored medical devices and surfaces.

Facile Synthesis and Characterization of L-Aspartic Acid Coated Iron Oxide Magnetic Nanoparticles (IONPs) For Biomedical Applications

Drug Research ◽  
2017 ◽  
Vol 68 (05) ◽  
pp. 280-285 ◽  
Author(s):  
Marziyeh Salehiabar ◽  
Hamed Nosrati ◽  
Soodabeh Davaran ◽  
Hossein Danafar ◽  
Hamidreza Manjili
Keyword(s):  
Electron Microscopy ◽  
Iron Oxide ◽  
Magnetic Nanoparticles ◽  
Aspartic Acid ◽  
Precipitation Method ◽  
Scanning Calorimetry ◽  
One Pot ◽  
Iron Oxide Magnetic Nanoparticles

AbstractNatural L-aspartic acid coated iron oxide magnetic nanoparticles (Asp@IONPs) were prepared by a one pot, in-situ and green co–precipitation method in an aqueous medium. Functionalized iron oxide magnetic nanoparticles (IONPs) were characterized by Vibrating Sample Magnetometer (VSM), X-ray diffraction (XRD), differential scanning calorimetry (DSC), Fourier transform infrared spectroscopy (FTIR), Scanning electron microscopy (SEM) and Transmission electron microscopy (TEM) techniques. Cellular toxicity of IONPs was also investigated on HEK-293 cell lines. The results showed that the zeta potential of Asp@IONPs was about −21.1 mV and the average size was 17.80±3.09 nm. Cell toxicity results show that as prepared IONPs are biocompatible. Asp@IONPs show the possibility of using these nanoparticles in the development of in vitro and in vivo biomedical fields due to do not possess a toxic effect, good ζ-potential and related small and narrow size distribution.

scholarly journals Novel Cubic Magnetite Nanoparticle Synthesis Using Room Temperature Ionic Liquid

2012 ◽  
Vol 9 (3) ◽  
pp. 1070-1076 ◽  
Author(s):  
M. Sundrarajan ◽  
M. Ramalakshmi
Keyword(s):  
Electron Microscopy ◽  
Ionic Liquid ◽  
Iron Oxide ◽  
Magnetite Nanoparticles ◽  
Room Temperature ◽  
Average Particle Size ◽  
Precipitation Method ◽  
Average Particle ◽  
Face Centered Cubic ◽  
Ft Ir

Room Temperature Ionic liquids are relatively more useful in the synthesis of inorganic nanostructured materials because of their unique properties. To synthesize the iron oxide nanoparticle in simple precipitation method, a novel ionic liquid was used as the greener medium and stabilizing agent namely “1-n-butyl-3-methylimidazolium trifluoromethane sulfonate [BMIM][TfO]”. The crystallinity, chemical structure, morphology and magnetic properties of the synthesized magnetite nanoparticles have been characterized by using X-ray diffraction (XRD), Fourier Transform Infrared (FT-IR), Scanning electron microscopy (SEM), Atomic force microscopy(AFM), Transmission electron microscopy (TEM) and Vibrating sample magnetometer (VSM) studies. The XRD study is divulge that the synthesized magnetite nanoparticles have inverse spinel face centered cubic structure. The FT-IR vibration peaks show the formation of Fe3O4nanoparticles, where the vibration peak for Fe-O is deliberately presence at 584 cm-1. The average particle size of the synthesized nanoparticles is found to be 35 nm. Homogeneously dispersed cubic shape with superstructure is found through SEM, AFM and TEM examination studies. The synthesized iron oxide nanoparticles have a high saturation magnetization value of 25 emu/g, which is very much useful for biomedical applications.

scholarly journals Functionalized Magnetic Nanoparticles and Their Effect onEscherichia coliandStaphylococcus aureus

2015 ◽  
Vol 2015 ◽  
pp. 1-10 ◽  
Author(s):  
Mohamed S. A. Darwish ◽  
Nhung H. A. Nguyen ◽  
Alena Ševců ◽  
Ivan Stibor
Keyword(s):  
Electron Microscopy ◽  
Magnetite Nanoparticles ◽  
Biological Effect ◽  
Bacterial Strains ◽  
Gram Negative ◽  
E Coli ◽  
Functionalized Magnetic Nanoparticles ◽  
Nanoparticle Morphology ◽  
Average Size ◽  
Scanning Electron

Magnetite (Fe3O4) nanoparticles were prepared using coprecipitation and subsequently surface-functionalized with 3-aminopropyltriethoxysilane (APTS), polyethylene glycol (PEG), and tetraethoxysilane (TEOS). Nanoparticle morphology was characterized using scanning electron microscopy, while structure and stability were assessed through infrared spectroscopy and zeta potential, respectively. Average size of the nanoparticles analysed by dynamic light scattering was 89 nm, 123 nm, 109 nm, and 130 nm for unmodified magnetite and APTS-, PEG-, and TEOS-modified magnetite nanoparticles, respectively. Biological effect was studied on two bacterial strains: Gram-negativeEscherichia coliCCM 3954 and Gram-positiveStaphylococcus aureusCCM 3953. Most of modified magnetite nanoparticles had a significant effect onS. aureusand not onE. coli, whereas PEG-magnetite nanoparticles displayed no significant effect on the growth rate of either bacteria.

scholarly journals Antibiofilm and Antifungal Activities of Laurelia sempervirens (Chilean laurel) Essential Oil

2021 ◽  
Vol In Press (In Press) ◽  
Author(s):  
Olga Lobos ◽  
Carlos Padilla ◽  
Andrea Barrera ◽  
Zoraya Lopez-Cabana ◽  
Claudia Mora ◽  
...  
Keyword(s):  
Minimum Inhibitory Concentration ◽  
Essential Oil ◽  
Inhibitory Concentration ◽  
Antimicrobial Properties ◽  
Inhibitory Effect ◽  
Diffusion Method ◽  
Antibiofilm Activity ◽  
Bacterial Strains ◽  
Pathogenic Yeast ◽  
Antifungal Properties

Background: Laurelia sempervirens, better known as Chilean laurel, is endemic to Chile. For many years, the leaves and branches of this plant have been popularly used as medicinal agents. However, its antifungal properties and antibiofilm activity against bacterial strains have not been studied. Objectives: To determine antibiofilm and antimicrobial properties of Chilean laurel essential oil on human pathogenic strains. Methods: The antifungal and antibacterial activities of Chilean laurel essential oil were evaluated through the agar diffusion method, and its minimum inhibitory concentration was determined using the standard microdilution method. Antibiofilm activity was examined based on the formation and disruption of bacterial biofilms and evidenced by the crystal violet technique. Results: The results showed important antimicrobial activity against human pathogenic yeast strains, and the minimum inhibitory concentration of Chilean laurel essential oil was 64 µg.mL-1 against Candida albicans. The essential oil also showed an important inhibitory effect against the formation of biofilms produced by Staphylococcus aureus, since it inhibited the formation of biofilms by over 50% at the concentration of 64 µg.mL-1. With increasing the essential oil’s concentration to 128 µg.mL-1, its antibiofilm activity increased by 60%. Conclusions: These results approve the domestic use of Chilean laurel essential oil as an antimicrobial agent and provide knowledge about the antibiofilm and antifungal properties of L. sempervirens.

scholarly journals MAPLE Coatings Embedded with Essential Oil-Conjugated Magnetite for Anti-Biofilm Applications

Materials ◽  
2021 ◽  
Vol 14 (7) ◽  
pp. 1612
Author(s):  
Oana Gherasim ◽  
Roxana Cristina Popescu ◽  
Valentina Grumezescu ◽  
George Dan Mogoșanu ◽  
Laurențiu Mogoantă ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Essential Oil ◽  
Composite Coatings ◽  
Grazing Incidence ◽  
Precipitation Method ◽  
Laser Evaporation ◽  
Experimental Conditions ◽  
Nanostructured Composite ◽  
In Vitro Biocompatibility

The present study reports on the development and evaluation of nanostructured composite coatings of polylactic acid (PLA) embedded with iron oxide nanoparticles (Fe3O4) modified with Eucalyptus (Eucalyptus globulus) essential oil. The co-precipitation method was employed to synthesize the magnetite particles conjugated with Eucalyptus natural antibiotic (Fe3O4@EG), while their composition and microstructure were investigated using grazing incidence X-ray diffraction (GIXRD), Fourier transform infrared spectroscopy (FT-IR), thermogravimetric analysis (TGA), transmission electron microscopy (TEM) and dynamic light scattering (DLS). The matrix-assisted pulsed laser evaporation (MAPLE) technique was further employed to obtain PLA/Fe3O4@EG thin films. Optimal experimental conditions for laser processing were established by complementary infrared microscopy (IRM) and scanning electron microscopy (SEM) investigations. The in vitro biocompatibility with eukaryote cells was proven using mesenchymal stem cells, while the anti-biofilm efficiency of composite PLA/Fe3O4@EG coatings was assessed against Gram-negative and Gram-positive pathogens.

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