scholarly journals Heat-Treated Bifidobacterium longum CECT-7347: A Whole-Cell Postbiotic with Antioxidant, Anti-Inflammatory, and Gut-Barrier Protection Properties

Antioxidants ◽  
2021 ◽  
Vol 10 (4) ◽  
pp. 536
Author(s):  
Patricia Martorell ◽  
Beatriz Alvarez ◽  
Silvia Llopis ◽  
Veronica Navarro ◽  
Pepa Ortiz ◽  
...  
Keyword(s):  
Functional Properties ◽  
Bacterial Colonization ◽  
Bifidobacterium Longum ◽  
Model Systems ◽  
Gut Health ◽  
Gut Barrier ◽  
Whole Cell ◽  
Heat Treated ◽  
Anti Inflammatory ◽  
Immune System Modulation

Non-viable preparations of probiotics, as whole-cell postbiotics, attract increasing interest because of their intrinsic technological stability, and their functional properties, such as immune system modulation, gut barrier maintenance, and protection against pathogens. However, reports on Bifidobacteria-derived postbiotics remain scarce. This study aims to demonstrate the functional properties of a heat-treated (HT), non-viable, Bifidobacterium longum strain, CECT-7347, a strain previously selected for its anti-inflammatory phenotype and ability to improve biomarkers of intestinal integrity in clinical trials. The study used the nematode Caenorhabditis elegans and HT-29 cell cultures as eukaryotic model systems. Our results show that HT-CECT-7347 preserves the capacity to protect against oxidative stress damage, while it also reduces acute inflammatory response and gut-barrier disruption, and inhibits bacterial colonization, by activating pathways related to innate immune function. These findings highlight the interest of the ingredient as a novel postbiotic and pave the way to broaden the range of HT-CECT-7347 applications in gut health.

scholarly journals Ingestion of probiotic (Lactobacillus helveticus and Bifidobacterium longum) alters intestinal microbial structure and behavioral expression following social defeat stress

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Katherine A. Partrick ◽  
Anna M. Rosenhauer ◽  
Jérémie Auger ◽  
Amanda R. Arnold ◽  
Nicole M. Ronczkowski ◽  
...  
Keyword(s):  
Social Stress ◽  
Bifidobacterium Longum ◽  
Social Defeat ◽  
Lactobacillus Helveticus ◽  
Rrna Gene ◽  
Social Avoidance ◽  
Social Defeat Stress ◽  
Microbial Structure ◽  
Microbial Composition ◽  
Anti Inflammatory

AbstractSocial stress exacerbates anxious and depressive behaviors in humans. Similarly, anxiety- and depressive-like behaviors are triggered by social stress in a variety of non-human animals. Here, we tested whether oral administration of the putative anxiolytic probiotic strains Lactobacillus helveticus R0052 and Bifidobacterium longum R0175 reduces the striking increase in anxiety-like behavior and changes in gut microbiota observed following social defeat stress in Syrian hamsters. We administered the probiotic at two different doses for 21 days, and 16S rRNA gene amplicon sequencing revealed a shift in microbial structure following probiotic administration at both doses, independently of stress. Probiotic administration at either dose increased anti-inflammatory cytokines IL-4, IL-5, and IL-10 compared to placebo. Surprisingly, probiotic administration at the low dose, equivalent to the one used in humans, significantly increased social avoidance and decreased social interaction. This behavioral change was associated with a reduction in microbial richness in this group. Together, these results demonstrate that probiotic administration alters gut microbial composition and may promote an anti-inflammatory profile but that these changes may not promote reductions in behavioral responses to social stress.

scholarly journals Anti-Platelet Properties of Apple Must/Skin Yeasts and of Their Fermented Apple Cider Products

Beverages ◽  
2021 ◽  
Vol 7 (3) ◽  
pp. 54
Author(s):  
Donal Moran ◽  
Mary Fleming ◽  
Eimear Daly ◽  
Natasha Gaughan ◽  
Ioannis Zabetakis ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Platelet Aggregation ◽  
Functional Properties ◽  
Human Platelet ◽  
Omega 3 ◽  
Apple Cider ◽  
Human Platelet Aggregation ◽  
Yeast Strains ◽  
Omega 6 ◽  
Anti Inflammatory

Alcoholic beverages like apple cider are considered functional beverages with several health benefits, when consumed in moderation, which are mainly attributed to their microbiota and the plethora of their bioactive compounds. Among them, bio-functional polar lipids (PL) have recently been found in apple cider, which despite low quantities, have exhibited strong anti-inflammatory and anti-platelet properties, while fermentation seems to affect the functionality of apple cider’s PL bioactives. The aim of the present study was to elaborate yeast strains isolated from the complex mixtures of apple surface and must yeasts for evaluating their effects on the anti-platelet functional properties of PL bioactives from their final fermented apple cider products. First, bio-functional PL were extracted and separated from the biomass of the different isolated apple surface/must yeast strains, and were further assessed for their anti-platelet potency against human platelet aggregation induced by the potent inflammatory and thrombotic mediator platelet-activating factor (PAF), or by a classic platelet agonist like adenosine diphopshate (ADP). Novel functional apple ciders were then produced from the fermentation of apple juice by elaborating the most bioactive and resilient yeast strains isolated from the apple must with optimum fermentation properties. PL bioactives extracted from these novel apple cider products were also further assessed for their anti-platelet properties against both the PAF and ADP pathways of human platelet aggregation. These novel cider products were found to contain PL bioactives with lower IC50 values (~40 μg) and thus increased anti-platelet potency against platelet aggregation induced by PAF and ADP. GC-MS analysis of the PL bioactives extracted from these novel apple ciders showed that apple cider PL bioactives are rich in monounsaturated fatty acids (MUFA) and polyunsaturated fatty acids (PUFA), such as the omega-6 linoleic acid (LA) and the omega-3 alpha linolenic acid (ALA), with favorably lower levels for their omega-6/omega-3 PUFA ratio, which further support the observed strong anti-platelet properties putative anti-inflammatory potency for the apple cider PL bioactives. However, further studies are needed in order to elucidate and fully characterize the apple yeast strains that can be utilized for increasing the anti-inflammatory, anti-platelet and cardioprotective functional properties of their fermented apple cider products.

The synergy of dietary supplements Lactobacillus salivarius LI01 and Bifidobacterium longum TC01 in alleviating liver failure in rats treated with d-galactosamine

2021 ◽  
Author(s):  
Aoxiang Zhuge ◽  
Shengjie Li ◽  
Yin Yuan ◽  
Bo Li ◽  
Lanjuan Li
Keyword(s):  
Liver Injury ◽  
Gut Microbiota ◽  
Liver Failure ◽  
Dietary Supplements ◽  
Bifidobacterium Longum ◽  
Gut Barrier ◽  
Lactobacillus Salivarius

L. salivarius LI01 and B. longum TC01 synergize in liver injury via altering gut microbiota and protecting gut barrier.

Herbal Medicine and Nutritional Supplements for Gut Health

2019 ◽  
pp. 175-192
Author(s):  
Kara Fitzgerald ◽  
Nikky Contractor
Keyword(s):  
Health Care ◽  
Herbal Medicine ◽  
Nutritional Supplements ◽  
Gut Health ◽  
Gastrointestinal Conditions ◽  
Current Therapies ◽  
Gastrointestinal Complaints ◽  
Inflammatory Drugs ◽  
Anti Inflammatory ◽  
Digestive Health

Gastrointestinal complaints are among the most common health considerations. Current therapies such as acid blockers, non-steroidal anti-inflammatory drugs (NSAIDS) and antibiotics are not without their challenges and adverse effects. Herbal medicine provides an array of anti-inflammatory, antimicrobial, antiemetic, and carminative plants that provide additional tools to the health care professional. Quality dietary and nutritional supplements containing extracts of herbs, prebiotics, or probiotics offer a standardized delivery system for these ingredients. Herbal medicine and dietary supplements have been demonstrated to be effective in various gastrointestinal conditions. This chapter will highlight the nutraceutical products that have been proven in clinical trials to treat and mitigate digestive health ailments.

scholarly journals Draft Genome Sequence of Bifidobacterium longum BB-79, a Strain That Produces Acid Exopolysaccharide

2018 ◽  
Vol 7 (19) ◽  
Author(s):  
Linyan Cao ◽  
Junlin Yu ◽  
Huahai Chen ◽  
Yeshi Yin
Keyword(s):  
Genome Sequence ◽  
Immune Modulation ◽  
Bacterial Colonization ◽  
Bifidobacterium Longum ◽  
Draft Genome ◽  
Draft Genome Sequence ◽  
Content Type

The surface exopolysaccharide of Bifidobacterium spp. was found to be involved in many processes, including bacterial colonization and host immune modulation.

scholarly journals Antibacterial and anti-inflammatory effects of lime (citrus aurantifolia) peel extract in mice balb/c induced salmonella typhi

2020 ◽  
Author(s):  
Vivien Novarina Kasim ◽  
Mochammad Hatta ◽  
Rosdiana Natzir ◽  
Veni Hadju ◽  
Yusminah Hala ◽  
...  
Keyword(s):  
Bacterial Colonization ◽  
Serum Levels ◽  
Salmonella Typhi ◽  
Negative Control ◽  
Citrus Aurantifolia ◽  
Post Test ◽  
Anti Inflammatory ◽  
Lime Peel ◽  
Maintenance Intervention ◽  
Peel Extract

Lime (Citrus aurantifolia) is a traditional plant that is widely used as antibacterial. This study to prove the effect of lime peel extract (LPE) on the growth of bacterial colonization of S. Typhi medited by activity of IL-6. True experimental pre-post test design, mice were divided into; LPE 510 mg/kgbw, LPE 750 mg/kgbw, positive and negative control. The examination was carried out 3 times, the 5th day before the intervention, the 10th day after the intervention and the 30th day after maintenance. Intervention LPE for 5 days can decreased the number of S. Typhi colonies, even maintenance for 20 days after the intervention showed no bacterial growth . IL-6 pro-inflammatory cytokine activity increased on examination day 5 after S.Typhi injection and decreased after intervention on day 10, significantly different between pre and post at all groups except negative controls (p=0.15). The speed of decrease in IL-6 levels was greatest at the LPE 750 mg/kgbw (velocity=-5.64%). LPE decreased serum levels of IL-6 and inhibit the growth of S. Typhi colony in mice Balb/c. LPE have potential as antibacterial and anti-inflammatory.

Fermented ginseng attenuates lipopolysaccharide-induced inflammatory responses by activating the TLR4/MAPK signaling pathway and remediating gut barrier

2021 ◽  
Author(s):  
Jingjing Fan ◽  
Sitong Liu ◽  
Zhiyi Ai ◽  
Yiying Chen ◽  
Yonghong Wang ◽  
...  
Keyword(s):  
Signaling Pathway ◽  
Inflammatory Responses ◽  
Mapk Signaling ◽  
Physiological Effect ◽  
Mapk Signaling Pathway ◽  
Gut Barrier ◽  
Fermented Ginseng ◽  
Anti Inflammatory

Generally, ginsenosides have the physiological effect of an anti-inflammatory immunity.

scholarly journals Fructans in the diet cause alterations of intestinal mucosal architecture, released mucins and mucosa-associated bifidobacteria in gnotobiotic rats

2003 ◽  
Vol 89 (5) ◽  
pp. 597-606 ◽  
Author(s):  
Brigitta Kleessen ◽  
Ludger Hartmann ◽  
Michael Blaut
Keyword(s):  
Bacterial Colonization ◽  
Bifidobacterium Longum ◽  
Distal Colon ◽  
Goblet Cells ◽  
Mucosal Barrier ◽  
Standard Diet ◽  
Mucus Layer ◽  
Faecal Flora ◽  
Health Maintenance ◽  
Distal Jejunum

The effects of fructans in the diet on the mucosal morphometry (height of villi, depth of the crypts, number of goblet cells), the thickness of the epithelial mucus layer and the histochemical composition of intestinal mucosubstances in the distal jejunum and the distal colon were investigated by comparing germ-free (GF) rats, rats harbouringBacteroides vulgatusandBifidobacterium longum(diassociated (DA) rats), and rats with a human faecal flora (HFA). The rats were fed either a commercial standard diet (ST) or ST + (50 g oligofructose (OF)–long-chain inulin (lcIN))/kg. Changes in total bacteria, bifidobacteria andBacteroides–Prevotellain response to feeding these diets were investigated by fluorescentin situhybridization with 16S rRNA-targeted probes both in intestinal contents (lumen bacteria) and tissue sections (mucosa-associated bacteria). The OF–lcIN-containing diet resulted in higher villi and deeper crypts in bacteria-associated, but not in GF rats. In DA and HFA rats, the colonic epithelial mucus layer was thicker and the numbers of the goblet cells were greater than in GF rats. These effects were enhanced by the OF–lcIN-containing diet. In both dietary groups, bacterial colonization of GF rats caused an increase in neutral mucins in the distal jejunum and colon. Bacteria-associated rats had more acidic mucins in the colon than GF rats, and the OF–lcIN-containing diet stimulated sulfomucins as the predominant type of acidic mucins, while sialomucins dominated in the ST-fed groups. The number of mucosa-associated bifidobacteria detected in the colon of DA and HFA rats was greater with OF–lcIN than ST (4·9 and 5·4v. 3·5 and 4·0 log10/mm2mucosal surface respectively), whereas the number of luminal bifidobacteria was only affected by fructans in DA rats.Bacteroidesdid not differ between the groups. The stabilisation of the gut mucosal barrier, either by changes in the mucosal architecture itself, in released mucins or by stimulation of mucosal bifidobacteria with fructans, could become an important topic in the treatment and prophylaxis of gastrointestinal disorders and health maintenance.

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