scholarly journals Comprehensive Biological and Chemical Evaluation of Two Seseli Species (S. gummiferum and S. transcaucasicum)

Antioxidants ◽  
2021 ◽  
Vol 10 (10) ◽  
pp. 1510
Author(s):  
Gokhan Zengin ◽  
Dejan Stojković ◽  
Mohamad Fawzi Mahomoodally ◽  
Bibi Sharmeen Jugreet ◽  
Mehmet Yavuz Paksoy ◽  
...  
Keyword(s):  
Methanolic Extract ◽  
Biological Activities ◽  
Radical Scavenging ◽  
Water Extract ◽  
Herbal Remedies ◽  
Total Phenolic ◽  
Hacat Cells ◽  
Medicinal Uses ◽  
Water Extracts ◽  
Methanolic Extracts

Seseli L. is one of the largest genera of the Apiaceae family widely known for their traditional uses as herbal remedies. In the present study, the methanolic and water extracts of two Seseli species, S. gummiferum and S. transcaucasicum were evaluated for their bioactive contents and biological activities. The total phenolic and flavonoid contents in the extracts ranged from 19.09 to 24.33 mg GAE/g and from 0.45 to10.09 mg RE/g, respectively. Moreover, while narcissin was detected as the most abundant component in the methanolic extract of S. transcaucasicum (261.40 µg/g), chlorogenic acid was identified as the major component in all the other extracts, although a high amount was also present in the methanolic S. transcaucasicum extract (107.48–243.12 µg/g). The total antioxidant capacity was also determined by the phosphomolybdenum assay (0.66–1.18 mM TE/g). Other antioxidant assays such as the radical scavenging assays (DPPH: 5.51–11.45 mg TE/g; ABTS: 43.46–51.91 mg TE/g), reducing assays (CUPRAC: 41.67–53.20 mg TE/g; FRAP: 31.26–34.14 mg TE/g), as well as the metal chelating activity assay (14.38–38.57 mg EDTAE/g) were conducted. All the extracts showed inhibitory potential against the enzyme’s amylase (0.12–0.78 mM ACAE/g), acetyl- and butyryl-cholinesterase (0.15–9.71 mg GALAE/g), while only the methanolic extracts acted as inhibitors of tyrosinase (107.15 and 109.37 mg KAE/g) and only the water extract of S. gummiferum displayed anti-glucosidase activity (0.13 mM ACAE/g). Interestingly, the methanolic extracts of both Seseli species showed lower cytotoxicity towards HaCaT cells (IC50: >500 µg/mL), compared to the water extracts (IC50: 267.8 and 321.41 µg/mL). Besides, only the methanolic extracts showed a slight wound healing effect (28.21 and 31.23%). All extracts showed antibacterial action against Staphylococcus lugdunensis (minimum inhibitory and bactericidal concentrations: 0.025–2 mg/mL). S. gummiferum methanolic extract, which exhibited the highest antibacterial potency was found to inhibit adhesion and invasion of S. lugdunensis to HaCaT cells as well. Taken together, this study demonstrated the two Seseli species to harbour interesting bioactive components, in particular polyphenolics and to exhibit several biological properties that could be further investigated for their potential exploitation as healing agents as supported by various traditional medicinal uses.

scholarly journals Phytochemical Constituents and Biological Activities of the Unexplored Plant Rhinanthus angustifolius subsp. grandiflorus

2021 ◽  
Vol 11 (19) ◽  
pp. 9162
Author(s):  
Leilei Zhang ◽  
Gokhan Zengin ◽  
Gabriele Rocchetti ◽  
Ismail Senkardes ◽  
Jugreet B. Sharmeen ◽  
...  
Keyword(s):  
High Resolution Mass Spectrometry ◽  
Biological Activities ◽  
Radical Scavenging ◽  
Water Extract ◽  
The Other ◽  
Total Phenolic ◽  
Water Extracts ◽  
Extraction Solvent ◽  
Rhinanthus Angustifolius ◽  
Untargeted Profiling

In the present study, a total of 12 extracts of Rhinanthus angustifolius subsp. grandiflorus, an understudied hemiparasitic species, were obtained using different extraction techniques, namely, homogenizer-assisted extraction (HAE), maceration (MAC), soxhlet (SOX), infusion, and solvents (ethyl acetate, methanol, ethanol, and water), and were evaluated for their in vitro antioxidant and enzyme-inhibiting properties. Additionally, untargeted profiling based on high-resolution mass spectrometry targeted different phytochemical classes, namely, polyphenols, terpenoids, and alkaloids. The highest total phenolic and flavonoid contents were detected using methanol as the extraction solvent. Multivariate statistics following the untargeted profiling revealed that the extraction solvent had a hierarchically higher impact than the extraction method when considering the recovery of bioactive compounds. The methanolic extracts displayed the highest radical-scavenging antioxidant capacity, as provided by CUPRAC and FRAP assays. On the other hand, the water extracts (MAC and HAE) and the infusion extract showed the highest activity as metal chelators (25.66–27.51 mg EDTAE/g). Similarly, the water extract obtained by HAE and the infusion extract revealed the highest phosphomolybdenum activity (3.92 ± 0.14 and 3.71 ± 0.01 mmol TE/g, respectively). The different extracts also exhibited different enzyme inhibition potentials. For instance, HAE and MAC ethanolic extracts inhibited only α-amylase (0.69 ± 0.01 and 0.70 ± 0.01 mmol ACAE/g), while all the other extracts showed a dual inhibition against both carbohydrate-hydrolyzing enzymes tested (i.e., α-amylase: 0.07–0.69 mmol ACAE/g; α-glucosidase: 0.03–1.30 mmol ACAE/g). Nevertheless, the other extracts inhibited acetyl-, butyryl-cholinesterases, or both; MAC–water extract displayed no inhibition against the enzymes. Additionally, all the studied extracts were found to inhibit tyrosinase, ranging from 10.62 to 52.80 mg KAE/g. In general, the water extracts showed weaker inhibition towards the enzymes than the other extracts. This study demonstrated that R. angustifolius is an excellent source of natural antioxidants and enzyme inhibitors that could be further investigated and exploited for pharmaceutical purposes.

scholarly journals Phytochemical, Antibacterial, and Antioxidant Activities of Anthurium Hookerii leaves Extracts

2019 ◽  
Vol 26 (3) ◽  
pp. 101
Author(s):  
Atmira Sariwati ◽  
Inayah Fitri ◽  
Adi Setyo Purnomo ◽  
Sri Fatmawati
Keyword(s):  
Ethyl Acetate ◽  
Radical Scavenging ◽  
Water Extract ◽  
Maximum Diameter ◽  
Diffusion Method ◽  
Total Phenolic ◽  
Total Phenol Content ◽  
Water Extracts ◽  
The Family ◽  
Antibacterial Potential

Many plants of the family of Araceae possess significant benefit as medicinal plants. Anthurium hookerii is herbaceous genus of the family of Araceae. A. hookerii leaves were extracted with five dissimilarity solvents (methanolic, water, ethyl acetate, n-hexane, and dichloromethane). The extracts were evaluated for their phytochemical, total phenolic contents, and antibacterial potential. The presences of tannins and saponins were found in all crude extracts. The steroid was only found in dichloromethane extract, whereas flavonoid was obtained in methanol and water extracts. Besides; methanol, ethyl acetate, water, and n-hexane extracts showed triterpenoid contents. Alkaloid presences in ethyl acetate, methanolic, dichloromethane, and water extracts. The total phenol content was examined by Follin-Ciocalteu assay, which varied from 9.52-76.56 mg/g GAE. The highest total phenolic was found in methanol extract. Antioxidant activity was calculated based on diphenyl picryl hydrazyl radical scavenging ability that showed the scavenging activity with range 7.24-66.11%, which the methanoilic extract have the excellent antioxidant potential (IC50 232.90 µg/ml). Antibacterial activity of leaves extracts of A. hookerii was screened based on disc diffusion method. Water extract showed the wide spectrum antibacterial potential. Klebsiella sp., Bacillus subtilis, Pripioni agnes, and Strepticoccus mutans with maximum diameter of inhibition zone 10.30, 14.20, 9.60, and 15.10 mm, respectively.

scholarly journals Free-radical Scavenging and Quantitative Estimation of Flavonoids from the Polar Extracts of Corchorus depressus Leaves

2021 ◽  
pp. 210-219
Author(s):  
Khuntia Tapas Kumar ◽  
Nanda Upendra Nath ◽  
Senapati Aswini Kumar
Keyword(s):  
Nitric Oxide ◽  
Antioxidant Activity ◽  
Phenolic Content ◽  
Methanolic Extract ◽  
Biological Activities ◽  
Radical Scavenging ◽  
Aluminium Chloride ◽  
Total Phenolic ◽  
Nitric Oxide Radical ◽  
Polar Extracts

Background: The investigation of total flavonoids and antioxidant activity of polar extracts of Corchorus depressus is the major aim of this study. As observed from ancient literatures and folkloric claims the plant Corchorus depressus worshipped by the married women of Odisha, India, in the rituals called as “Jama Jutia”, possesses different biological activities including antioxidant property. Methods: The diphenyl picryl hydrazine, hydroxyl radical and nitric oxide radical scavenging methods were performed for measurement of the antioxidant activity at different extracts. The flavonoid and phenolic content of the extracts were determined by using aluminium chloride and Folin-Ciocalteau’s reagent (FCR) methods respectively. Results: The results for estimation of total phenolic content (mg/ 100 g) expressed as gallic acid equivalent (GAE) and total flavonoid (mg/ 100 g) in weight of quercetin equivalent (QE) was highest in methanolic extract 78.46 and  21.2 respectively, followed by 18.18 mg/100 g in GAE and 1.80 mg/100 g in QE for aqueous extract. Conclusion: The methanolic extract of C. depressus at 100µg/ml showed highest DPPH, hydroxyl and nitric oxide radical scavenging activity and this activity may be attributed to the presence of saponins and flavonoids as detected in the extract.

scholarly journals Quantification of Bioactive Compounds and Evaluation of the Antioxidant Activity of Carissa edulis Valh (Apocynaceae) Leaves

2019 ◽  
Vol 2019 ◽  
pp. 1-9 ◽  
Author(s):  
Sabine Adeline Fanta Yadang ◽  
Germain Taiwe Sotoing ◽  
Katoucha Sidoine Ngatcha Zouakeu ◽  
Muhammad Ahsan Khan ◽  
Gabriel Agbor Agbor ◽  
...  
Keyword(s):  
Antioxidant Capacity ◽  
Bioactive Compounds ◽  
Total Phenolic Content ◽  
Hplc Analysis ◽  
Phenolic Content ◽  
Methanolic Extract ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Total Phenolic ◽  
Methanolic Extracts

Carissa edulis is a plant used in the management of oxidative stress and inflammatory related disorders such as malaria, rheumatism inflammation, and cardiovascular diseases. The present study evaluates the total phenolic content, antioxidant capacity (DPPH, ABTS, and FRAP), and the bioactive compounds present in the various extracts of C. edulis (HEC, MEC, AEC, and PC). An HPLC analysis determined the different compounds present in the extracts. High concentration of total phenolic content was observed in aqueous and methanolic extracts more than in the hydroethanolic extract though not significantly different. Flavonoids were higher in the hydroethanolic and methanolic extracts, respectively, with 14.84 mg RE/g extract and 12.02 mg RE/g extract. Tannins were also found in large amounts in the same two extracts with 26.76 mg TAE/g extract and 34.67 mg TEE/g extract. The percentage radical scavenging activity DPPH ranged between 58.63% and 94.67% for aqueous extract and for ABTS between 51.39% and 94.12% for the methanolic extract. The highest FRAP was obtained in the methanolic extract (6.73 g AAE/100 g extract). HPLC analysis revealed the presence of quercetin, rutin, and gallic acid in the different extracts. C. edulis represents a potential source of bioactive components with antioxidant capacity.

scholarly journals Phytochemical evaluation and pharmacognostic standardization of Syzygium palghatense endemic to Western Ghats

2021 ◽  
Vol 7 (1) ◽  
Author(s):  
V. R. Snehalatha ◽  
A. R. Rasmi
Keyword(s):  
Western Ghats ◽  
Methanolic Extract ◽  
Biological Activities ◽  
Biological Properties ◽  
Biologically Active ◽  
Total Phenolic ◽  
Significant Activity ◽  
Pharmacological Characterization ◽  
Methanolic Extracts ◽  
Pharmacological Potential

Abstract Background The plants belonging to the genus Syzygium has received much attention since ancient times due to its multidimensional uses for mankind. These plants in general, reported to contain a wide array of phytomolecules with a broad spectrum of biological activities that include antihypoglycemic, antioxidant, anti-inflammatory, anticarcinogenic, and antihypertensive properties. The pharmacological potential of S. palghatense which is endemic to Western Ghats has not yet been investigated. The present study was intended to examine the potential benefits of the leaves and bark of S. palghatense. Results Morpho-anatomical studies of S. palghatense provided useful details for the identification. The pharmacological characterization of the plants shows significant results in their studied parameters. The total phenolic and flavonoid contents were higher in methanolic extracts of leaves and bark of S. palghatense. Methanolic extract of the leaves and bark have superior antioxidant capacity when compared with the chloroform, ethyl acetate, and distilled water extracts. A significant result in alpha amylase and alpha glucosidase enzyme activity was exhibited when antidiabetic study was performed. Conclusion The current study showed that the methanolic extract of S. palghatense leaves and bark have significant pharmacognostic properties and potential antioxidant and antidiabetic compounds. Phytochemical studies portray the presence of several biologically active secondary metabolites, including essential oil, alkaloid, glycosides, carbohydrate, tannins, reducing sugar, phenolics, and saponins, which may be the reason for its biological properties. As compared to bark, leaves of S. palghatense possess significant activity toward all the biological properties tested.

scholarly journals Propiedades antioxidante y antiglicosilante de extractos de Diplotaxis tenuifolia

Bionatura ◽  
2019 ◽  
Vol 4 (2) ◽  
pp. 852-855
Author(s):  
Romina B Parada ◽  
Marisol Vallejo ◽  
Emilio R Marguet
Keyword(s):  
Ascorbic Acid ◽  
Maillard Reaction ◽  
Dry Matter ◽  
Biological Activities ◽  
Antioxidant Properties ◽  
Radical Scavenging ◽  
Water Extract ◽  
Edible Plant ◽  
Water Extracts ◽  
Trolox Equivalent

The goal of this study was to investigate phenolic contents and biological activities in leaves of Diplotaxis tenuifolia collected in Patagonia (Argentina). Anti-glycation activities were tested by bovine serum albumin-glucose (BSA-glu) and Maillard reaction models. Only in the case of water extract of dry matter, Maillard reaction could be 40 % inhibited. The remaining results do not allow make definitive conclusions. Water extracts exhibited higher polyphenol content than methanolic ones (1074.25 and 647.5 mg Gallic acid equivalent/100 g of dry matter, respectively). On the other hand, flavonoid content was comparable between the water and methanolic extracts (218.2 and 213.4 mg quercetin equivalent/100 g of dry matter, respectively). Regarding antioxidant properties, CUPRAC (Cupric reducing antioxidant capacity) showed values of 655.6 mg Trolox equivalent/100 g of dry matter and 749.6 mg ascorbic acid equivalent/100 g of dry matter for water extracts and 1045,2 mg Trolox equivalent/100 g of dry matter and 1203,9 mg ascorbic acid equivalent/100 g of dry matter for methanolic ones. DPPH (2,2-diphenyl-1-picrylhydrazyl) radical scavenging assay displayed values of 203,3 mg Trolox equivalent/100 g of dry matter and 180.0 mg ascorbic acid equivalent/100 g of dry matter for water extracts, and 97.7 mg Trolox equivalent/100 g of dry matter and 89,3 mg ascorbic acid equivalent/100 g of dry matter for methanolic ones. The results obtained highlight the antioxidative potential of D. tenuifolia collected in extreme regions like Patagonia. This edible plant is an antioxidant-rich source that should be considered as functional food, its consumption is recommendable to prevent cell damage by scavenging deleterious free radicals.

scholarly journals Effect of extraction temperature and solvent type on the bioactive potential of Ocimum gratissimum L. extracts

2020 ◽  
Vol 10 (1) ◽  
Author(s):  
Confidence Onyebuchi ◽  
Doğa Kavaz
Keyword(s):  
Biological Activities ◽  
Radical Scavenging ◽  
Total Phenolic ◽  
Order Kinetic ◽  
Extraction Temperature ◽  
Ocimum Gratissimum ◽  
Methanolic Extracts ◽  
Bioactive Properties ◽  
Solvent Type ◽  
Wide Range

AbstractOcimum gratissimum is a shrub that belongs to the Lamiaceae family of plants. Despite the known biological activities and ethnomedicinal applications, comparative evaluation of the effects of different extraction techniques on the chemical and bioactive properties of O. gratissimum extracts has not yet been performed. This study adopted different analytical techniques to determine the effect of extraction temperature and solvent type on the phytochemical and bioactive properties of O. gratissimum extracts. Chemical profiling showed increased concentrations of compounds for both the ethanolic and methanolic extracts compared to the water extracts. The results also revealed that the extraction temperature had an effect on the total phenolic content and radical-scavenging properties of the different extracts. The antioxidant kinetic modeling achieved the best fit when using the second-order kinetic model. Methanolic extracts had the highest levels of antibacterial activity against Escherichia coli, Bacillus cereus, Staphylococcus aureus, and Salmonella typhimurium. At high concentrations, all extracts lowered the viability of the breast cancer cell line MDA-MB-231. In conclusion, the chemical and bioactive properties of all extracts showed significant dependence on the extraction temperature and solvent type. With proper extraction methods, they boast a wide range of promising applications in the medical, pharmaceutical, and food industries.

Biological activities of Saussurea lappa antioxidants recovered by solid-liquid, ultrasound and Ired-Irrad®

2020 ◽  
Vol 16 ◽  
Author(s):  
Hiba N. Rajha ◽  
Gisèle El Khoury ◽  
Nada El Darra ◽  
Karim Raafat ◽  
Espérance Debs ◽  
...  
Keyword(s):  
Response Surface Methodology ◽  
Response Surface ◽  
Biological Activities ◽  
Radical Scavenging Activity ◽  
Maximum Yield ◽  
Radical Scavenging ◽  
Total Phenolic ◽  
Medicinal Uses ◽  
Saussurea Lappa ◽  
Solid Liquid

Background: Saussurea lappa is a traditionally well-known plant appreciated for its biological activities and medicinal uses. Objective: In the present study, the recovery of antioxidants from Saussurea lappa was optimized using Response Surface Methodology (RSM). The efficiency of a newly-patented infrared (IR) technology, Ired-Irrad®, was compared to that of the emerging ultrasound method (US) and the conventional solid liquid water bath (WB) extraction. Methods: The effects of time (t) and temperature (T), mostly known to affect the extraction process, were tested on maximizing the total phenolic compounds concentration (TPC) and the radical scavenging activity (AA). Response surface methodology was used for the optimization process. Results: A multiple response optimization of both time (t) and temperature (T) was conducted, showing the best extraction conditions to be for WB: t= 43.86 min, T=33.79°C, for US: t= 65.47 min, T= 57.97°C and for IR: t= 42.5 min, T=34.19°C. The quantity of the optimally extracted polyphenols by WB, US and IR; as well as many of their bioactivities were compared. IR extraction gave the highest yield of TPC (15.3 mg GAE/g DM) followed by US (14.8 mg GAE/g DM) and lastly WB (13.9 mg GAE/g DM). The highest antioxidant and antiradical activities were also obtained by the IR treatment. The optimal IR extract inhibited the growth of Staphylococcus aureus and Escherichia coli up to 65 and 35%, respectively. Moreover, all Saussurea lappa extracts (WB, US and IR) inhibited up to 96% the production of Aflatoxin B1 (AFB1) by Aspergillus flavus. Conclusion: Our findings on the extraction of antioxidants from Saussurea lappa demonstrated that IR technology is an efficient novel method that can be used to extract the maximum yield of polyphenols, with the highest antioxidant, antiradical and antibacterial activities.

scholarly journals Phytochemicals Levels and Antioxidant Capacities of Figs Flowers Fruits

2020 ◽  
Vol 8 (1) ◽  
pp. 29-35
Author(s):  
Messaoudi Mohammed ◽  
Merah Maroua
Keyword(s):  
Free Radical ◽  
Antioxidant Capacity ◽  
Methanolic Extract ◽  
Radical Scavenging ◽  
Free Radical Scavengers ◽  
Total Phenolic ◽  
Phytochemical Analysis ◽  
Total Anthocyanin ◽  
Methanolic Extracts ◽  
Almost All

Since antiquity, phenolic compounds produced by plants were known as free radical scavengers and as powerful antioxidants. Huge interest has been made by researchers to the traditional uses of medicinal plants against illnesses related to oxidative stress. This study measures the correlation that can be existed between the antioxidant capacity and phytochemical s levels of four varieties of Ficus carica fruits, figs flowers or "Bakor" as called locally in Algeria. Therefore, extracts were assessed for determining their antioxidative potentials using both test of total antioxidant capacity and DPPH (1,1-diphenyl-2-picrylhydrazyl) free radical scavenging test followed by quantitative phytochemical analysis to estimate the total flavonoid level (TFL), the total phenolic level (TPL), the total anthocyanin level (TAL) and the condensed tannins level of plants methanolic extracts. A positive correlation was observed between phenolics content and the antioxidant capacity of figs flowers methanol extracts. The methanolic extract of Bechar (MeOH Var.2) chelated 87,9± 1,23 % of the DPPH free radical with IC50 value equal to 0,185 mg/g DW. A high antioxidant ability of almost all extracts is, probably, related to the appreciable rates of flavonoids, phenolics and tannins showed by those fig extracts. The highest value of phenolics level was detected among the variety 1 methanolic extract of Bechar (MeOH Var.1) of 10,4 mg GAE/g DW.

scholarly journals Antioxidant capacity and antioxidants of strawberry, blackberry, and raspberry leaves

2011 ◽  
Vol 29 (No. 2) ◽  
pp. 181-189 ◽  
Author(s):  
L. Buřičová ◽  
M. Andjelkovic ◽  
A. Čermáková ◽  
Z. Réblová ◽  
O. Jurček ◽  
...  
Keyword(s):  
Antioxidant Capacity ◽  
Ellagic Acid ◽  
Radical Scavenging ◽  
Water Extract ◽  
Rubus Idaeus ◽  
Total Phenolic ◽  
Water Extracts ◽  
Antioxidant Capacities ◽  
Dpph Method ◽  
Radical Scavenging Ability

The total phenolic content (Folin-Ciocalteu method), free radical scavenging ability expressed as DPPH value, ferric reducing antioxidant capacity (FRAP), and oxygen radical absorbance capacity (ORAC) were determined in water extracts of leaves from Rosaceae family plants (Fragaria vesca L., Rubus fructicosus L., and Rubus idaeus L.). The antioxidant capacities of the extracts (in the order of the above mentioned methods) were 73.6–88.9%, 60.1–71.4%, 49.7–78.0% respectively, and 45.3–66.5% of that of green tea water extract. Further, the presence of 15 compounds (gallic acid, rutin, ellagic acid, caffeic acid, p-coumaric acid, quercetin, kaempferol, myricetin, quercetin-3-d-glucoside, ascorbic acid, (+)-catechin, (–)-epicatechin, epicatechingallate, epigallocatechin, procyanidin B1) was studied by HPLC-ECD and their antioxidant capacities were compared to the antioxidant capacity of the extracts. Out of the compounds studied, mostly (+)-catechin, ellagic acid, and (–)-epicatechin participated in the antioxidant capacities of the studied plant leaves water extracts. The antioxidant capacity of leaves infusions (determined by DPPH method) was lower than those of red wines and tea infusions, but comparable to the antioxidant capacities of white wines and fruit beverages.

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