Seasonality Modulates the Cellular Antioxidant Activity and Antiproliferative Effect of Sonoran Desert Propolis

The main chemical composition and pharmacological potential of propolis from arid and semi-arid regions of the Sonoran Desert have been previously reported. Caborca propolis (CP), from an arid zone of the Sonoran Desert, has shown a polyphenolic profile that suggests a mixed plant origin, presenting poplar-type markers, as well as a 6-methoxylated flavonoid, xanthomicrol, characteristic of Asteraceae plants. In addition, CP has shown significant antioxidant properties and antiproliferative activity on cancer cells. In this study, we analyzed the influence of collection time on the chemical constitution, antiproliferative activity and protective capacity of CP against reactive oxygen species (ROS), by using HPLC–UV–diode array detection (DAD) analysis, 3-(4,5-dimethylthiazol-2-yl)-2,5-Dimethyltetrazoliumbromide (MTT) and 2,2-diphenyl-1-picryl-hydrazyl (DPPH) assays, as well as cellular antioxidant activity (CAA) assay on murine B-cell lymphoma M12.C3.F6 cells. HPLC–UV–DAD analyses of seasonally collected CP (one-year period) revealed quantitative differences among the most abundant CP constituents: pinocembrin, galangin, chrysin and pinobanksin-3-O-acetate. Though all seasonal samples of CP induced an antiproliferative effect in M12.C3.F6 cells, CP from autumn showed the highest inhibitory activity (IC50: 5.9 ± 0.6 µg/mL). The DPPH assay pointed out that CP collected in autumn presented the highest antioxidant potential (IC50: 58.8 ± 6.7 µg/mL), followed by winter (65.7 ± 12.2 µg/mL) and spring (67.0 ± 7.5 µg/mL); meanwhile, the summer sample showed a lesser antioxidant capacity (IC50: 98.7 ± 2.5 µg/mL). The CAA assay demonstrated that CP induced a significant protective effect against ROS production elicited by H2O2 in M12.C3.F6 cells. Pretreatment of M12.C3.F6 cells with CP from spring and autumn (25 and 50 µg/mL for 1 h) showed the highest reduction in intracellular ROS induced by H2O2 (1 and 5 mM). These results indicate that the antiproliferative effect and cellular antioxidant activity of CP are modulated by quantitative fluctuations in its polyphenolic profile due to its collection time.

Download Full-text

Effect of in vitro digestion of yerba mate ( Ilex paraguariensis A. St. Hil.) extract on the cellular antioxidant activity, antiproliferative activity and cytotoxicity toward HepG2 cells

Food Research International ◽

10.1016/j.foodres.2015.05.004 ◽

2015 ◽

Vol 77 ◽

pp. 257-263 ◽

Cited By ~ 22

Author(s):

Brunna Cristina Bremer Boaventura ◽

Renata Dias de Mello Castanho Amboni ◽

Edson Luiz da Silva ◽

Elane Schwinden Prudencio ◽

Patricia Faria Di Pietro ◽

...

Keyword(s):

Antioxidant Activity ◽

Antiproliferative Activity ◽

Hepg2 Cells ◽

In Vitro Digestion ◽

Ilex Paraguariensis ◽

Yerba Mate ◽

Cellular Antioxidant Activity

Download Full-text

The Effect of Adding the Leaves and Fruits of Morus alba to Rape Honey on Its Antioxidant Properties, Polyphenolic Profile, and Amylase Activity

Molecules ◽

10.3390/molecules25010084 ◽

2019 ◽

Vol 25 (1) ◽

pp. 84 ◽

Cited By ~ 2

Author(s):

Monika Tomczyk ◽

Michał Miłek ◽

Ewelina Sidor ◽

Ireneusz Kapusta ◽

Wojciech Litwińczuk ◽

...

Keyword(s):

Antioxidant Activity ◽

Phenolic Content ◽

Antioxidant Properties ◽

Inhibitory Effect ◽

Flavonoid Glycosides ◽

Total Phenolic ◽

Antioxidant Compounds ◽

Mulberry Leaves ◽

Hydrolyzing Enzymes ◽

Polyphenolic Profile

Two various species of mulberry (Morus sp.) were selected to enrich rape honey with dried leaves or lyophilized fruits (4% w/v). Finally, fruits and leaves of the ‘Ukraińska’ clone were introduced into the honey during creaming in concentrations from 1 to 4% w/v. The total phenolic content, antioxidant activity, anthocyanins content, and polyphenolic profile were tested in plant extracts and enriched honeys. Moreover, α-glucosidase, β-galactosidase, and diastase activities were investigated in honeys. For mulberry extracts, chlorogenic acid isomers and rutin were considered main antioxidant compounds. The antioxidant activity of honey enriched with mulberry leaves increased even more than 50 times, due to introducing numerous phenolic acids and flavonoid glycosides. A significant decrease in the diastase activity in honey depending on the content of added mulberry leaves (almost 50% decrease in the case of 4% addition) was found, suggesting the inhibitory effect of honey with mulberry leaves against carbohydrate hydrolyzing enzymes.

Download Full-text

Evaluation of cellular antioxidant activity of selected species of Caralluma and Boucerosia on cell lines

International Journal of Applied Sciences and Biotechnology ◽

10.3126/ijasbt.v2i1.9649 ◽

2014 ◽

Vol 2 (1) ◽

pp. 83-87 ◽

Cited By ~ 2

Author(s):

Madhuri Vajha ◽

Siva Rama Krishna Chillara

Keyword(s):

Antioxidant Activity ◽

Cell Lines ◽

Plant Extracts ◽

Inhibitory Concentration ◽

Antioxidant Properties ◽

Raw 264.7 Cells ◽

Cellular Antioxidant Activity ◽

Methanolic Extracts ◽

Normal Metabolism ◽

Promising Source

Objective: The aim of the present work was designed to study cellular antioxidant properties of selected plant extracts of Caralluma and Boucerosia on cell lines. Materials and Methods: The present study was carried out in four species of Caralluma R.Br. such as Caralluma adscendens (Roxb.) R. Brown var. attenuata (Wight) Grav. & Mayur. (CAA), Caralluma adscendens (Roxb.) R. Brown var. fimbriata (Wall.) Gravely & Mayur. (CAF), Caralluma stalagmifera C.E.C. Fisch. (CS) and Caralluma stalagmifera C.E.C. Fisch. var. longipetala Karupp. & Pull. (CSL) and as well as two species of Boucerosia Wight & Arn. such as Boucerosia lasiantha Wight. (BL) and Boucerosia umbellata (Haw.) Wight & Arn. (BU). The antioxidant efficacy was evaluated by ability of antioxidant molecules in the extract to scavenge ROS produced by normal metabolism by cells, observed by the oxidation of 2, 7- Dichloro fluorescein diacetate to dichlorofluorescein, followed by decrease in fluorescence intensity. Results: Cellular antioxidant activity was demonstrated by the inhibitory concentration 50% (IC50) of reactive oxygen species (ROS). The percentage ROS inhibition in Raw 264.7 cells when treated with different concentrations of methanolic extracts showed variations among selected species. Conclusion: The plant extracts were shown to be effective for antioxidant potential and can be a promising source of natural antioxidants.DOI: http://dx.doi.org/10.3126/ijasbt.v2i1.9649Int J Appl Sci Biotechnol, Vol. 2(1): 83-87

Download Full-text

Phytochemical content, cellular antioxidant activity and antiproliferative activity of Adinandra nitida tea (Shiyacha) infusion subjected to in vitro gastrointestinal digestion

RSC Advances ◽

10.1039/c7ra07429h ◽

2017 ◽

Vol 7 (80) ◽

pp. 50430-50440 ◽

Cited By ~ 12

Author(s):

Yongsheng Chen ◽

Xiang Ma ◽

Xiong Fu ◽

Rian Yan

Keyword(s):

Antioxidant Activity ◽

Southeast Asia ◽

Antiproliferative Activity ◽

Gastrointestinal Digestion ◽

Cellular Antioxidant Activity ◽

Phytochemical Content ◽

In Vitro Gastrointestinal Digestion

Adinandra nitida tea (Shiyacha) is a traditional eminent and flourishing tea with a long history in Southeast Asia.

Download Full-text

Fig (Ficus carica) Liquid Co-Products as New Potential Functional Ingredient: Physico-Chemical and In Vitro Antioxidant Properties

Natural Product Communications ◽

10.1177/1934578x1501000721 ◽

2015 ◽

Vol 10 (7) ◽

pp. 1934578X1501000

Author(s):

Manuel Viuda-Martos ◽

Esther Sendra ◽

Estrella Sayas ◽

José A. Pérez-Alvarez ◽

Juana Fernández-López

Keyword(s):

Antioxidant Activity ◽

Organic Acids ◽

Sugar Content ◽

Antioxidant Properties ◽

Total Phenolic ◽

Total Anthocyanin ◽

Physico Chemical ◽

Food Matrices ◽

Polyphenolic Profile

The aims of this study were to determine (i) the physico-chemical characteristics, (ii) the phenolic, flavonoid and anthocyanins profile, (iii) the organic acids and sugar content, and (iv) the antioxidant properties of the liquid co-product generated by the figs (FLC) processing industry in order to set standards for its applications as a potential ingredient of food products. The liquid co-products obtained from the peel and pulp of two fig varieties (colar and cuello de dama) were used. The polyphenolic profile and the organic acids and sugar were determined by HPLC. For the antioxidant activity, four different test systems were used (DPPH, FRAP, TBARS and FIC). The total phenolic, total flavonoid and total anthocyanin contents were also determined. The FLC from peel presented higher total phenolic, flavonoid and anthocyanin contents than FLC from pulp. The FLC obtained from peel and pulp had a high content of sugars, mainly glucose and fructose. As regards antioxidant activity, at all concentrations and with all methods, FLC obtained from peel presented higher antioxidant activity than the pulp FLC. FLC obtained from the peel and pulp could be an alternative for use as a natural preservative in food matrices due to its broad antioxidant activity.

Download Full-text

Study of the quality and antioxidant properties of tomatoes (Solanum lycopersicum L.) under different postharvest and dehydration conditions

RECUBRIMIENTO COMESTIBLE CON BASE DE PIÑON MEXICANO (Jatropha curcas) SOBRE LA CALIDAD DE CHAYOTE MINIMAMENTE PROCESADO ◽

10.32854/agrop.vi.1662 ◽

2020 ◽

Vol 13 (9) ◽

Author(s):

María Lorena Luna-Guevara ◽

Teresita González-Sánchez ◽

Adriana Delgado-Alvarado ◽

María Elena Ramos-Cassellis ◽

José Guillermo Pérez-Luna ◽

...

Keyword(s):

Antioxidant Activity ◽

Rural Communities ◽

Antioxidant Properties ◽

Titratable Acidity ◽

Physicochemical Characteristics ◽

Soluble Solids ◽

Convective Drying ◽

Tomato Fruits ◽

Solanum Lycopersicum L ◽

Storage Temperatures

Objective: To study the effect of storage temperatures and dehydration conditions (solar and convective drying; SD, CD), on the quality, physicochemical parameters and antioxidant properties of tomato fruits. Methodology: The physicochemical characteristics pH, titratable acidity, soluble solids (°Bx) and color parameters (L*, a* and b*), were evaluated. The lycopene, carotenoids and antioxidant activity percentages retention of tomatoes fruits stored at 7 and 22 ° C for 5 days and subjected to SD (Temperature (T) of 67 °C and luminescence of 685 lum/sqf) and CD (T 70 °C, flow rates 0.5, 1.0 and 1.5 m/s), were analyzed. Results: The fruits reached humidities of 17 and 15% for SD and CD. The parameters pH, °Bx, L*, a*, b* were highest with 22°C and CD (1.5 m/s). The value of the carotenoids was higher in fruits stored at 7 ° C and subjected to CD (1.0 and 1.5 m/s) and SD with values of 83.85, 85.98 and 99.43%, respectively. The CD (0.5 m/s) and SD improved lycopene (94.37 and 95.14%) and the antioxidant activity with values of 73.06 and 97.21%. Implications: The application of solar dehydration depends on luminescence condition; however, it is inexpensive and environmentally friendly alternative. Conclusions: The results derived in a viable alternative for the conservation and commercialization of tomato fruits in rural communities

Download Full-text

Antioxidant activity of cookiessupplemented with sugarbeet dietary fiber

Sugar Industry ◽

10.36961/si11177 ◽

2011 ◽

pp. 151-157 ◽

Cited By ~ 2

Author(s):

Marijana B. Saka ◽

Julianna F. Gyura ◽

Aleksandra Mišan ◽

Zita I. Šereš ◽

Biljana S. Pajin ◽

...

Keyword(s):

Antioxidant Activity ◽

Shelf Life ◽

Dietary Fiber ◽

Wheat Flour ◽

Room Temperature ◽

Antioxidant Activities ◽

Radical Scavenging Activity ◽

Antioxidant Properties ◽

Radical Scavenging ◽

Functional Characteristics

The antioxidant activity of cookies prepared by the addition of sugarbeet dietary fibers was investigated in order to estimate their influence on functional characteristics and shelf-life of cookies. Treated fiber (TF) was obtained from sugarbeet by extraction with sulfurous acid (75 °C at pH = 5.7during 60 min) and treatment with hydrogen peroxide (20 g/LH2O2 at pH = 11 during 24 h). The fiber obtained was dried (80 °C), ground and sieved. TF was investigated in comparison with commercially available Fibrex®. The cookies were prepared by the addition of 0, 7, 9 and 11% of sugarbeet dietary fiber as a substitute for wheat flour in the formulation of cookies. The antioxidant properties of cookies were tested every 7 days using a DPPH (1,1-diphenyl-2-picrylhydrazyl) radical scavenging activity test during 6 weeks of storage at room temperature (23 ± 1 ºC). The obtained results indicated that substitution of wheat flour with Fibrex® in the formulation of cookies upgraded the antioxidant activity, i.e. the functional characteristics of Fibrex®-enriched cookies and could prolong their shelf-life. In contrast, TF did not increase the antioxidant activity of TF-enriched cookies. The better antioxidant activities of Fibrex®-enriched cookies could be attributed to the presence of ferulic acid.

Download Full-text

Evaluation of diuretic and antioxidant properties in aqueous bark and fruit extracts of pine

Current Drug Discovery Technologies ◽

10.2174/1570163817666200206105231 ◽

2020 ◽

Vol 17 ◽

Cited By ~ 1

Author(s):

Hadi Shariati ◽

Mohammad Hassanpour ◽

Gholamreza Sharifzadeh ◽

Asghar Zarban ◽

Saeed Samarghandian ◽

...

Keyword(s):

Antioxidant Activity ◽

Phenolic Content ◽

Antioxidant Properties ◽

Pine Bark ◽

Male Rats ◽

Total Phenolic ◽

Dependent Manner ◽

Aqueous Extracts ◽

Antioxidant Power ◽

Dose Dependent

Objective: The present study has been carried out to evaluate the diuretic and antioxidant properties of pine herb in an animal model. Materials and Methods: 45 adult male rats were randomly divided into nine groups including: groups I (the negative control), groups II (positive control, furosemide 10 mg/kg), groups III to VIII (treatment groups received 100, 200, 400 mg/kg of the aqueous extracts of bark and fruit) and group IX received the combination of aqueous extract of bark (100 mg/kg) and the fruit (100 mg/kg). The urine output, glomerular filtration rate (GFR), electrolytes, urea, and creatinine levels were evaluated . Furthermore, the phenolic content and antioxidant activity of both extracts were also assessed using 2, 2-diphenyl-1-picrylhydrazyl (DPPH), ferric reducing antioxidant power (FRAP) and Folin–Ciocalteu methods. Results: The aqueous extracts of the pine bark and fruit increased the urinary output in a dose-dependent manner. The combination of the two extracts compared to the other extracts alone significantly increased the serum potassium level. This study also showed each extract increase creatinine clearance in a dose-dependent manner (p<0.01 and p<0.05). The increase of GFR in the combination group was not significant. The current data showed a significant increase in the total phenolic content in pine bark extract in compared with the fruit extract. Conclusion: The pine bark and fruit can be useful in the prevention and treatment of kidney stones due to the high antioxidant activity.

Download Full-text

Effects of Greek Pomegranate Extracts in the Antioxidant Properties and Storage Stability of Kefir

Current Bioactive Compounds ◽

10.2174/1573407214666180808113450 ◽

2019 ◽

Vol 15 (4) ◽

pp. 437-441 ◽

Cited By ~ 1

Author(s):

Vasiliki Lagouri ◽

Georgia Dimitreli ◽

Aikatarini Kouvatsi

Keyword(s):

Antioxidant Activity ◽

Dairy Products ◽

Storage Time ◽

Storage Stability ◽

Flow Behavior ◽

Antioxidant Properties ◽

Flow Behavior Index ◽

Ph Values ◽

Dpph Activity ◽

Behavior Index

Background: Oxidation reactions are known to shorten the shelf life and cause damage to foods rich in fat, such as dairy products. One way to limit oxidation and increase the shelf life of fermented dairy products is to use natural antioxidants. The aim of this study was to examine the effect of adding pomegranate extracts in the antioxidant properties, rheological characteristics and the storage stability of the fermented product of kefir. Methods: The Pomegranate Juice (PGJ) and Peel Extracts (PGPE) (5%, 10% w/v) were added to kefir and the antioxidant properties were evaluated by using the methods of radical scavenging activity (DPPH) and Ferric Reducing Antioxidant Power Activity (FRAP). Spectrophotometric and instrumental methods were used to determine the Total Phenols (TPs), pH values, viscosity and flow behavioral index values of enriched with pomegranate kefir samples. The same properties were tested when kefir samples stored at 4°C for 7, 14, 21 and 28 days. Results: The addition of PGJ and PGPE results in an increase in the antioxidant activity (DPPH, FRAP) and total phenol content (TPs) of kefir samples. Increasing the concentration of the added PGJ and PGPE, results in an increase in the TP content and the DPPH activity of kefir. As far as the storage time is concerned, the results showed an increase in the amount of TP at 7th day and a reduction in the DPPH activity in the 14th day of storage. In contrary to the DPPH method, the increase in storage time has resulted in a reduction in antioxidant activity by the FRAP method. The addition of PGJ and PGPE in kefir results in a decrease in pH values while the pH of kefir samples increased during storage at 4°C for 28 days. The addition of PGJ and PGPE to kefir samples results to a decrease in viscosity and an increase in the flow behavior index. Increasing storage time results in increased flow behavior index of kefir samples. Conclusion: The addition of PGJ and PGPE increased the antioxidant activity and total phenols of the kefir product and preserved its properties during the total storage time of 28 days at 4°C.

Download Full-text

Effect of particle size on phytochemical composition and antioxidant properties of Sargassum cristaefolium ethanol extract

Scientific Reports ◽

10.1038/s41598-021-95769-y ◽

2021 ◽

Vol 11 (1) ◽

Author(s):

E. S. Prasedya ◽

A. Frediansyah ◽

N. W. R. Martyasari ◽

B. K. Ilhami ◽

A. S. Abidin ◽

...

Keyword(s):

Antioxidant Activity ◽

Particle Size ◽

Antioxidant Activities ◽

Antioxidant Properties ◽

Ethanol Extract ◽

Total Phenolic ◽

Particle Sizes ◽

Epicatechin Gallate ◽

Particle Size Reduction ◽

Phytochemical Composition

AbstractSample particle size is an important parameter in the solid–liquid extraction system of natural products for obtaining their bioactive compounds. This study evaluates the effect of sample particle size on the phytochemical composition and antioxidant activity of brown macroalgae Sargassum cristaefolium. The crude ethanol extract was extracted from dried powders of S.cristeafolium with various particle sizes (> 4000 µm, > 250 µm, > 125 µm, > 45 µm, and < 45 µm). The ethanolic extracts of S.cristaefolium were analysed for Total Phenolic Content (TPC), Total Flavonoid Content (TFC), phenolic compound concentration and antioxidant activities. The extract yield and phytochemical composition were more abundant in smaller particle sizes. Furthermore, the TPC (14.19 ± 2.08 mg GAE/g extract to 43.27 ± 2.56 mg GAE/g extract) and TFC (9.6 ± 1.8 mg QE/g extract to 70.27 ± 3.59 mg QE/g extract) values also significantly increased as particle sizes decreased. In addition, phenolic compounds epicatechin (EC), epicatechin gallate (ECG), epigallocatechin (EGC), and Epigallocatechin gallate (EGCG) concentration were frequently increased in samples of smaller particle sizes based on two-way ANOVA and Tukey’s multiple comparison analysis. These results correlate with the significantly stronger antioxidant activity in samples with smaller particle sizes. The smallest particle size (< 45 µm) demonstrated the strongest antioxidant activity based on DPPH, ABTS, hydroxyl assay and FRAP. In addition, ramp function graph evaluates the desired particle size for maximum phytochemical composition and antioxidant activity is 44 µm. In conclusion, current results show the importance of particle size reduction of macroalgae samples to increase the effectivity of its biological activity.

Download Full-text