scholarly journals Antidepressant-Like Effects of Ethanol Extract of Ziziphus jujuba Mill Seeds in Mice

2020 ◽  
Vol 10 (20) ◽  
pp. 7374
Author(s):  
Jong Min Oh ◽  
Moongi Ji ◽  
Mi-Jin Lee ◽  
Geum Seok Jeong ◽  
Man-Jeong Paik ◽  
...  
Keyword(s):  
Ethanol Extract ◽  
Tail Suspension Test ◽  
Positive Control ◽  
Negative Control ◽  
Mild Stress ◽  
Dependent Manner ◽  
Behavioral Tests ◽  
Bdnf Expression ◽  
Ziziphus Jujuba ◽  
Negative Controls

The antidepressant-like activity of ethanol extract of Ziziphus jujuba Mill var. spinosa seeds (Semen Ziziphi Spinosae, SZS) was investigated by behavioral tests, such as a forced swimming test (FST), a tail-suspension test (TST), and an open field test (OFT), using mice exposed to chronic unpredictable mild stress (CUMS). In the TST, immobility times of the extract-treated groups E100 and E300 (CUMS + 100 and 300 mg/kg extract, respectively) were significantly decreased in a dose-dependent manner compared with the negative control (CUMS; p < 0.01, though those of E100 and E300 were higher than those of the positive control (CUMS + 15 mg/kg fluoxetine). In the FST, immobility times of E100 and E300 were decreased compared to the normal control. In the OFT, total and zone distances of E100 and E300 were significantly higher than those of negative controls (p < 0.01) with a dose dependency. In liquid chromatography-tandem mass spectrometry (LC-MS/MS) analysis after behavioral tests, norepinephrine (NE) and 5-hydroxytryptamine (5-HT) levels in the hippocampus tissues of E100 and E300 were significantly higher than those of negative controls. Brain-derived neurotrophic factor (BDNF) levels in the hippocampus tissues of E100 and E300 were higher than those of negative controls. From these results, the SZS ethanol extract exhibited significant antidepressant-like effects via immobility decrease, distance increase, hippocampal NE and 5-HT increase, and BDNF expression. These results suggest that the extract could be a potential antidepressant agent.

scholarly journals KULIT BUAH JERUK LIMAU (Citrus amblycarpa (Hassk.) Osche) SEBAGAI ANALGESIK

Jurnal Kimia ◽  
2020 ◽  
pp. 24
Author(s):  
R. A. I. K. Maharani ◽  
N. K. Cahyaningsih ◽  
M. D. Abimanyu ◽  
K. W. Astuti
Keyword(s):  
Body Weight ◽  
Analgesic Activity ◽  
Treatment Options ◽  
Ethanol Extract ◽  
Positive Control ◽  
Negative Control ◽  
Fruit Peel ◽  
Hot Plate ◽  
Inflammatory Drugs ◽  
Negative Controls

Nonsteroidal anti-inflammatory drugs (NSAIDs) are the treatment options for relieving pain. However, long-term use can trigger gastrointestinal bleeding. Therefore, alternative analgesics which have the same therapeutic effect with lower side effects are needed. Limau (Citrus amblycarpa) is an empirical drug for tingling and cramping. The aim of the study is to determine the analgesic activity of ethanol extract of C. amblycarpa fruit peel. The method used in testing analgesic activity is the Hot Plate method. The study was conducted by dividing 30 mice into 6 groups. The group given CMC-Na 1% was used as a negative control, the group given suspension of sodium diclofenac dose of 6.5 mg/kg of body weight was used as a positive control, and the group given suspension of ethanol extract of C. amblycarpa fruit peel with dose variations 100, 300 and 600 mg/kg of body weight. The test animals were placed on top of the Hot Plate with a temperature of 70°C at 30 minutes after giving suspension test and the response time of mice to heat was observed every 30 minutes for 3 hours with cut off time 15 second. Based on the test results, it can be concluded that the administration of ethanol extract of C. amblycarpa fruit peel with 100, 300 and 600 mg/kg of body weight gave analgesic activity on mice compared to the negative controls (CMC-Na 1%).   Keywords: C. amblycarpa, Fruit Peel, Analgesics, Hot Plate 

scholarly journals NEPHROPROTECTIVE ACTIVITY OF ETHANOL EXTRACT OF CURCUMA MANGGA VAL. IN PARACETAMOL-INDUCED MALE MICE

2018 ◽  
Vol 11 (13) ◽  
pp. 126
Author(s):  
Rosita Rosita ◽  
Yuandani Yuandani ◽  
Marianne Marianne
Keyword(s):  
Creatinine Level ◽  
Cell Damage ◽  
Ethanol Extract ◽  
Positive Control ◽  
Negative Control ◽  
Dependent Manner ◽  
Male Mice ◽  
Treatment Groups ◽  
Level Measurement ◽  
Nephroprotective Activity

Objective: This study aimed to evaluate nephroprotective activity of Curcuma mangga in paracetamol-induced male mice.Methods: Male mice were divided into several groups including normal control, negative, positive, and treatment groups. Treatment groups were orally administered with C. mangga extract at doses of 100, 200, and 400 mg/kg bw for 7 days. On day 7after 1 h of treatment, the mice were induced with paracetamol 1.05 g/kg bw. Serum creatinine level measurement and histopathotlogy study were performed at the end of experiment.Results: C. mangga extract was able to inhibit the increase of creatinine level and showed a significantly different from negative control (p<0.05) and did not different significantly from positive control (p>0.05). The result was supported by histopathology examination which did not show any cell damage. The nephroprotective effect of C. mangga was in a dose-dependent manner. C. mangga extract at dose of 400 mg/kg bw depicted the strongest nephroprotective effect.Conclusion: C. mangga extract was able to protect mice kidney induced by paracetamol.

scholarly journals Evaluation of Antidiarrheal Activity of the Ethanol Extract Leucaena leucocephala (Lam) de Wit Seed

2020 ◽  
Vol 8 (A) ◽  
pp. 278-282
Author(s):  
Saddam Husein ◽  
Marline Nainggolan ◽  
Yuandani Yuandani ◽  
Irzal Fanany
Keyword(s):  
Leucaena Leucocephala ◽  
Ethanol Extract ◽  
Positive Control ◽  
Negative Control ◽  
Intestinal Transit ◽  
Dependent Manner ◽  
Stool Weight ◽  
Time Frequency ◽  
Antidiarrheal Activity ◽  
Ethanol Extracts

BACKGROUND: Leucaena leucocephala belongs to the Leguminosae/Fabaceae family. L. leucocephala seeds contain alkaloids, flavonoids, and tannins which according to the previous research have antidiarrhea activity. AIM: This study was investigate the antidiarrheal activity of the ethanol extracts of Leucaena leucocephala (Lam) de Wit seeds induced by oleum ricini and intestinal transit methods for rats. MATERIALS AND METHODS: L. leucocephala seeds were extracted by maceration with 80% ethanol. Evaluation of antidiarrheal extract activity was performed by induction of oleum ricini and intestinal transit methods. The extract at dose doses of 50, 100, 200, and 400 mg/kg BW was orally administered to the animals 1 h after induction by oleum ricini. Then diarrhea time, frequency, consistency, stool weight, and duration of diarrhea were observed every 30 min for 6 h. In determining the intestinal transit method, a percentage of the distance of the Chinese ink determined. This study was used positive control as Loperamide (1 mg/kg BW) and 0.5% Na-CMC as a negative control. RESULT: In diarrhea induced by castor oil, L. leucocephala seed extract at doses of 100, 200, and 400 mg/kg bw has been shown to significantly delay the onset of diarrhea, reduce diarrhea frequency, stool weight and duration of diarrhea compared with Na CMC as a negative control (p < 0.05). The extract at a dose of 400 mg/kg bw did not differ significantly from loperamide as positive control (p > 0.05). In this study, L. leucocephala extract reduced the distance traveled by Chinese ink in the intestine but only at a dose of 400 mg/kg bw which has a comparable activity with loperamide significantly . The antidiarrheal activity of extract showed at a dose dependent manner. CONCLUSION: The ethanol extract of L. leucocephala seeds has antidiarrheal activity which supports its use in folk medicines.

scholarly journals EFFECT OF CURCUMA XANTHORRHIZA ROXB. ETHANOL EXTRACT ON THE VIABILITY OF STREPTOCOCCUS MUTANS AND STREPTOCOCCUS SANGUINIS DUAL-SPECIES BIOFILMS

2019 ◽  
pp. 75-78
Author(s):  
FARIDA ERVINTARI ◽  
RIA PUSPITAWATI ◽  
SRI UTAMI
Keyword(s):  
Streptococcus Mutans ◽  
Bacterial Culture ◽  
Ethanol Extract ◽  
Positive Control ◽  
Negative Control ◽  
Streptococcus Sanguinis ◽  
Curcuma Xanthorrhiza ◽  
Ethanol Extracts ◽  
Negative Controls

Objective: This study aimed to determine the effect of ethanol Curcuma extract on the viability of S. mutans and Streptococcus sanguinis in a dualspeciesin vitro biofilm model.Methods: Dual-species biofilms of S. mutans and S. sanguinis were exposed to ethanol Curcuma extract at various concentrations. The sample ofsaliva was gathered from healthy volunteers. Chlorhexidine 0.2% was used as a positive control, and bacterial culture without intervention servedas a negative control. The total suspensions of 10−as were prepared for S. mutans and S. sanguinis cells. The bacteria were incubated for 20 h (activematuration phase) and 24 h (maturation phase).Results: The result showed decreased S. mutans and S. sanguinis viability after exposure to 0.2%–25% Curcuma ethanol extracts during the activeaccumulation and maturation phases. The decrease in bacterial viability was significantly different in all concentrations of Curcuma ethanol extractscompared with negative controls (p<0.05) in the active accumulation and maturation phases.Conclusion: Temulawak ethanol extract (starting at 0.2%) can decrease the viability of S. mutans and S. sanguinis in a dual species in vitro biofilmmodel during the accumulation and maturation phases.

scholarly journals Antidepressant-Like Effects of Cordycepin in a Mice Model of Chronic Unpredictable Mild Stress

2014 ◽  
Vol 2014 ◽  
pp. 1-9 ◽  
Author(s):  
Zhang Tianzhu ◽  
Yang Shihai ◽  
Du Juan
Keyword(s):  
Preference Test ◽  
Antidepressant Activity ◽  
Tail Suspension Test ◽  
Positive Control ◽  
Mild Stress ◽  
Chronic Unpredictable Mild Stress ◽  
Mice Model ◽  
Bdnf Expression ◽  
Beneficial Effects ◽  
Control Drug

Cordycepin (3′-deoxyadenosine), a major bioactive component isolated fromCordyceps militaris, has multiple pharmacological activities. This study is attempted to investigate whether cordycepin (COR) possesses beneficial effects on chronic unpredictable mild stress- (CUMS-) induced behavioral deficits (depression-like behaviors) and explore the possible mechanisms. ICR mice were subjected to chronic unpredictable mild stress for 42 consecutive days. Then, COR and fluoxetine (FLU, positive control drug) were administered for 21 consecutive days at the last three weeks of CUMS procedure. The classical behavioral tests, open field test (OFT), sucrose preference test (SPT), tail suspension test (TST), and forced swimming test (FST), were applied to evaluate the antidepressant effects of COR. Then the serotonin (5-HT) and noradrenaline (NE) concentrations in hippocampal were evaluated by HPLC; tumor necrosis factor-α(TNF-α) and interleukin-6 (IL-6) in hippocampal were evaluated, and the proteins of TNF-α, IL-6, NF-κBP65 5-HTreceptor (5-HTR), and brain-derived neurotrophic factor (BDNF) in hippocampal were evaluated by Western blot. Our results indicated that 6 weeks of CUMS exposure induced significant depression-like behavior, with low 5-HT and NE levels, high TNF-αand IL-6 in brain and high hippocampal TNF-α, IL-6, P-NF-κBP65, and 5-HTR levels, and low BDNF expression levels. Whereas, chronic COR (20, 40 mg/kg) treatments reversed the behavioral deficiency induced by CUMS exposure, treatment with COR normalized the change of TNF-α, IL-6, 5-HT, and NE levels, which demonstrated that COR could partially restore CUMS-induced 5-HT receptor impairments and inflammation. Besides, hippocampal BDNF expressions were also upregulated after COR treatments. In conclusion, COR remarkably improved depression-like behavior in CUMS mice and its antidepressant activity is mediated, at least in part, by the upregulating BDNF and downregulating 5-HTR levels and inflammation in hippocampus.

UJI AKTIVITAS HEPATOPROTEKTOR KOMBINASI EKSTRAK ETANOL DAUN PANDAN WANGI (Pandanus amaryllifolius roxb) DAN KAYU MANIS (Cinnamomum burmanii) TERHADAP KADAR MDA YANG DIINDUKSI PARASETAMOL

2020 ◽  
pp. 68-73
Author(s):  
Yuni Asri Mulatsih Agami ◽  
Eka Wisnu Kusuma
Keyword(s):  
Oxidative Stress ◽  
Ethanol Extract ◽  
Optimal Dose ◽  
Experimental Methods ◽  
Positive Control ◽  
Negative Control ◽  
Male Rats ◽  
Negative Group ◽  
Ic50 Value ◽  
Dose Combination

Kasus penyakit hati semakin meningkat seiring penggunaan senyawa hepatotoksin salah satunya karena penggunaan parasetamol dengan dosis berlebih. Hal tersebut dapat meningkatkan produksi radikal bebas sehingga memicu terjadinya stress oksidatif yang dapat menimbulkan kerusakan jaringan yang ditandai dengan peningkatan kadar Malondialdehyde (MDA). Stress oksidatif dapat diatasi dengan antioksidan dari berbagai tanaman. Kulit kayu manis memiliki aktivitas antioksidan dengan nilai IC50 53ppm dan daun pandan wangi 39,7%  Penelitian ini bertujuan untuk mengetahui aktivitas kombinasi ekstrak etanol daun pandan wangi dan kayu manis dalam menurunkan kadar MDA. tikus yang diinduksi parasetamol. Penelitian menggunakan metode eksperimental, dilakukan selama 9 hari dengan 30 ekor tikus jantan dibagi menjadi 6 Kelompok, yaitu: Normal diberi aquadest, Kontrol Positif diberi silimarin 100 mg/kgBB, Kontrol Negatif diberi CMC-Na 0,05%, serta 3 kelompok lainnya diberi kombinasi ekstrak daun pandan wangi:kayu manis berturut-turut dosis I (25:75), dosis II (50:50), dosis III (75:25). Semua kelompok diinduksi parasetamol 2,5 g/kgBB pada hari ke-7  setelah 30 menit perlakuan, kecuali kelompok normal. Pada hari ke 9 dilakukan pengukuran kadar MDA dengan metode TBARs menggunakan spektrofotometri. Pemberian kombinasi ekstrak etanol daun pandan wangi dan kayu manis dapat menurunkan kadar MDA dengan kombinasi dosis yang paling optimal adalah 75:25 berdasarkan statistik dengan nilai signifikan 0,000<0,05 dibandingkan dengan kelompok negatif.    Cases of liver disease have increased with the use of hepatotoxin compounds, one of which is due to the use of paracetamol with excessive doses. This can increase the production of free radicals so that it triggers oxidative stress which can cause tissue damage which is characterized by increased levels of Malondialdehyde (MDA). Oxidative stress can be overcome with antioxidants from various plants. Cinnamomum burmanii has antioxidant activity with IC50 value of 53ppm and Pandanus amarrylifolius 39.7%. This study aims to determine the combined activity of ethanol extract of Pandanus amarrylifolius and Cinnamomum burmanii  in reducing MDA levels. Paracetamol-induced rats. Research using experimental methods, conducted for 9 days with 30 male rats divided into 6 groups, namely: Normal given aquadest, Positive Control were given silimarin 100 mg / kgBB, Negative Control was given CMC-Na 0.05%, and 3 other groups were given a combination of Pandanus amarrylifolius extract: Cinnamomum burmanii dose I (25:75), dose II (50:50), dose III (75:25). All groups induced paracetamol 2.5 g / kgBB on the 7th day after 30 minutes of treatment, except the normal group. On the 9th day MDA levels were measured using the TBARs method using spectrophotometry. Giving a combination of Pandanus amarrylifolius and Cinnamomum burmanii ethanol extract can reduce MDA levels with the most optimal dose combination is 75:25 based on statistics with a significant value of 0,000<0.05 compared with the negative group.

Efek Ekstrak Etanol Kulit Petai (Parkia speciosa Hassk) Terhadap Penurunan Kadar Glukosa Darah Mencit Jantan

2018 ◽  
Vol 3 (1) ◽  
pp. 1
Author(s):  
Verawaty Verawaty ◽  
Dhea Claudia Novel
Keyword(s):  
Blood Glucose ◽  
Ethanol Extract ◽  
Negative Control Group ◽  
Positive Control ◽  
Negative Control ◽  
Control Group ◽  
Male Mice ◽  
Glucose Levels ◽  
The Third ◽  
Blood Glucose Levels

<p>Penelitian ini bertujuan untuk melihat pengaruh pemberian ekstrak etanol kulit petai (Parkia speciosa Hassk) terhadap penurunan kadar glukosa darah mencit jantan yang diinduksi aloksan. Hewan percobaan dibagi atas 5 kelompok diantaranya kelompok kontrol negatif, kelompok kontrol positif,dosis I (280 mg/kgBB mencit), dosis II (560 mg/kg BB mencit), dosis III (840 mg/kg BB mencit). Penelitian dilakukan selama 21 hari. Persentase penurunan kadar glukosa darah mencit jantan setelah diberikan ekstrak etanol kulit petai pada hari ke-21 adalah dosis I (77,52 %) lebih besar dibandingkan dengan dosis II (69,5 %) dan dosis III (73,37 %). Data yang diperoleh dianalisis dengan uji Two Way Anova dengan program SPSS 17. Hasil penelitian ini menunjukkan bahwa pemberian ekstrak etanol kulit petai untuk tiga variasi dosis menyatakan perbedaan yang bermakna secara statistik terhadap penurunan kadar glukosa darah mencit jantan.</p><p><em>Petai (Parkia speciosa Hassk) has a compound β-sitosterol and stigmasterol that have efficacy to decreased blood glucose levels. This study aimed to determine the effect of ethanol extract of petai peel for decrease blood glucose levels of male mice induced by alloxan. Experimental animals were divided into 5 groups including negative control group, positive control group, the first dose (280 mg/kg in mice), the second dose (560 mg/kg in mice), the third dose (840 mg/kg in mice). The study was conducted for 21 days. After 21 days, the result found that the percentage of blood glucose levels after the male mice given the ethanol extract of petai peel was, the first dose (77.52%) biger than the second dose (69.5%) and the third dose (73.37%). The data obtained were analyzed by Two Way ANOVA using SPSS 17. The results showed that have signicantly difference between three dose variation of ethanol extract of petai peel in blood glucose levels.</em></p>

scholarly journals Antihyperglycemic activity of Centella asiatica (L.) Urb. leaf ethanol extract SNEDDS in zebrafish (Danio rerio)

2021 ◽  
Vol 19 (1) ◽  
pp. 184-188
Author(s):  
Farida Hayati ◽  
Lutfi Chabib ◽  
Faiza Dea Sekarraras ◽  
Wan Syarifah Faizah
Keyword(s):  
Glucose Solution ◽  
Fasting Blood Glucose ◽  
Centella Asiatica ◽  
Ethanol Extract ◽  
Tween 80 ◽  
Positive Control ◽  
Negative Control ◽  
Antidiabetic Activity ◽  
Peg 400 ◽  
Good Preparation

Abstract This study aimed to identify the effectiveness of SNEDDS of Pegagan Leaf Ethanol Extract (PLE) to reduce fasting blood glucose (FBG) levels in zebrafish. Centella asiatica (L.) Urb. or pegagan is among the medicinal plants widely used to treat diabetes in Indonesia. Maceration was employed with 70% ethanol to obtain a viscous extract for the formulation of SNEDDS with Capryol 90, Tween 80, and PEG 400 (1:6:3). Antihyperglycemic testing was conducted on five groups, consisting of normal, positive control, negative control, P I treatment, and P II treatment. On Day 1, all except the normal group was induced with 300 mg alloxan and soaked in 2% glucose solution for 7 days. On day 8, the treatment consisted of 25 mg/2 L metformin for the positive control, 100 mg/2 L SNEDDS for P I, 200 mg/2 L SNEDDS for P II, and no treatment for the negative control. The SNEDDS characterization obtained 100.6 ± 3.12 nm particle size and −7.93 ± 0.66 mV zeta potential, indicating that the SNEDDS had fulfilled the requirements of good preparation. The antidiabetic activity test found a 69.90% decline in FBG levels in 100 mg/2 L SNEDDS and 72.20% in 200 mg/2 L SNEDDS.

scholarly journals Evaluation of ionic degradation and slot corrosion of metallic brackets by the action of different dentifrices

2013 ◽  
Vol 18 (1) ◽  
pp. 86-93
Author(s):  
Gustavo Antônio Martins Brandão ◽  
Rafael Menezes Simas ◽  
Leandro Moreira de Almeida ◽  
Juliana Melo da Silva ◽  
Marcelo de Castro Meneghim ◽  
...  
Keyword(s):  
Chemical Composition ◽  
Artificial Saliva ◽  
Positive Control ◽  
Negative Control ◽  
Sem Analysis ◽  
Wilcoxon Test ◽  
Surface Polishing ◽  
Aluminium Ion ◽  
Negative Controls

OBJECTIVE: To evaluate the in vitro ionic degradation and slot base corrosion of metallic brackets subjected to brushing with dentifrices, through analysis of chemical composition by Energy Dispersive Spectroscopy (EDS) and qualitative analysis by Scanning Electron Microscopy (SEM). METHODS: Thirty eight brackets were selected and randomly divided into four experimental groups (n = 7). Two groups (n = 5) worked as positive and negative controls. Simulated orthodontic braces were assembled using 0.019 x 0.025-in stainless steel wires and elastomeric rings. The groups were divided according to surface treatment: G1 (Máxima Proteção Anticáries®); G2 (Total 12®); G3 (Sensitive®); G4 (Branqueador®); Positive control (artificial saliva) and Negative control (no treatment). Twenty eight brushing cycles were performed and evaluations were made before (T0) and after (T1) experiment. RESULTS: The Wilcoxon test showed no difference in ionic concentrations of titanium (Ti), chromium (Cr), iron (Fe) and nickel (Ni) between groups. G2 presented significant reduction (p < 0.05) in the concentration of aluminium ion (Al). Groups G3 and G4 presented significant increase (p < 0.05) in the concentration of aluminium ion. The SEM analysis showed increased characteristics indicative of corrosion on groups G2, G3 and G4. CONCLUSION: The EDS analysis revealed that control groups and G1 did not suffer alterations on the chemical composition. G2 presented degradation in the amount of Al ion. G3 and G4 suffered increase in the concentration of Al. The immersion in artificial saliva and the dentifrice Máxima Proteção Anticáries® did not alter the surface polishing. The dentifrices Total 12®, Sensitive® and Branqueador® altered the surface polishing.

Effect of Chayote (Sechium Edule Jacq. Swartz) Extract on Level of Interleukin-8 in Wistar Rats with Aspirin-Induced Gastritis

2020 ◽  
Author(s):  
Hendrika Andriana Silitonga ◽  
◽  
Gontar Alamsyah Siregar ◽  
Rosita Juwita Sembiring ◽  
Marline Nainggolan ◽  
...  
Keyword(s):  
Ethyl Acetate ◽  
Wistar Rats ◽  
Ethanol Extract ◽  
Ethyl Acetate Fraction ◽  
Negative Control Group ◽  
Positive Control ◽  
Negative Control ◽  
Interleukin 8 ◽  
Control Group ◽  
Sechium Edule

ABSTRACT Background: Recent studies showed that Interleukin-8 (IL-8), activated cytokine immune response which plays an important role in the development of acute and chronic gastritis. Harmless anti-inflammatory therapeutic alternatives have been proposed, for example, the consumption of Sechium Edule Jacq. Swartz (chayote). Antioxidant (flavonoid) and cell regeneration (alkaloid) agents were found in chayote. This study aimed to determine the effect of chayote Sechium Edule Jacq. Swartz extracts on the level of IL-8 in Wistar rats with aspirin- induced gastritis. Subjects and Method: This was a randomized controlled trial (RCT) conducted at the laboratory of Mathematics and Natural Science, Universitas Sumatra Utara from January to February 2020. A total of 35 male Wistar rats was selected for this study and randomly allocated into 7 groups: (1) Negative control; (2) Positive control; (3) 100 mg/ kg BW chayote ethanol extract ; (4) 200 mg/kg BW chayote ethanol extract; (5) 100 mg/ kg BW chayote ethyl acetate fraction; (6) 200 mg/kg BW chayote ethyl acetate fraction; and (7) 20 mg omeprazole. The rats in positive control and treatment groups were induced with aspirin (200mg/ kg BW). The negative control group received no intervention. The dependent variable was level of IL-8 measured by ELISA. The independent variables were treatment status. The data were analyzed by One Way Anova and post hoc test. Results: The mean differences of IL-8 level were not statistically significant between study groups (p= 0.327). Mean of IL-8 level was higher in positive control group (Mean= 160.80; SD= 6.90) than in negative control group (Mean= 141.20; SD= 10.98). The lowest IL-8 level was in 100mg/ kg BW chayote ethanol extract group (Mean= 149.94; SD= 40.4), followed by 200mg/ kg BW (Mean= 152.4; SD= 30.73) and 100mg/ kg BW (Mean= 164.60; SD= 25.04) chayote ethyl acetate fraction groups, 20 mg omeprazole group (Mean= 170.60; SD= 21.58), and 200 mg/ kg BW chayote ethanol extract group (Mean= 176.80; SD= 10.98). Conclusion: The low dose (100mg/ kg BW) chayote ethanol extract has the most potential antiinflammation effect on in vitro gastritis with the lowest IL-8 level of all doses of chayote ethanol extract, chayote ethyl acetate fraction, and omeprazole. Keywords: antiinflammation, IL-8, chayote ethanol extract, ethyl acetate fraction, omeprazole, aspirin induced gastritis Correspondence: Hendrika Andriana Silitonga. Department of Histology, Faculty of Medicine, Universitas Methodist Indonesia. Email: [email protected]. Mobile: +6281361430688. DOI: https://doi.org/10.26911/the7thicph.05.35

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