scholarly journals Assessment of the Suitability of Methods for Testing the Antioxidant Activity of Anti-Aging Creams

2021 ◽  
Vol 11 (4) ◽  
pp. 1358
Author(s):  
Belma Imamović ◽  
Irmela Ivazović ◽  
Amra Alispahić ◽  
Ervina Bečić ◽  
Mirza Dedić ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Free Radicals ◽  
Antioxidant Capacity ◽  
Aging Process ◽  
Skin Aging ◽  
Plant Pigments ◽  
Spectrophotometric Methods ◽  
Measurement Results ◽  
Sensitive Method

Anti-aging cosmetics are often sought after in order to slow down the aging process. Free radicals are one of the main causes of skin aging, and therefore antioxidants are used in anti-aging cosmetics. The aim of this study was to investigate which method is the most suitable for determining the antioxidant capacity of these products. Having samples extracted, the antioxidant capacity of the extracts obtained was determined by the following spectrophotometric methods: DPPH, Folin-Ciocalteu, FRAP, the ABTS method and the ferroion chelation method with ferrosine. The antioxidant capacity of the samples varied depending on the extract type and the method used. DPPH and ferroion chelation measurements with ferrosine were carried out in the part of the spectrum where plant pigments absorb. These pigments are often found in anti-aging products affecting these methods measurement results. The Folin-Ciocalteu method is suitable for researching the antioxidant capacity of hydrophilic extracts, but not lipophilic ones, where turbidity and the formation of a gelled ring occur. The FRAP method revealed similar results for all the samples and proved to be less sensitive than the others. The ABTS method for both types of extracts has proven to be the most suitable and sensitive method for determining the antioxidant capacity of anti-aging products.

scholarly journals Potency of Black Soybean (Glycine max (L.) Merr) Extract and Daidzein as Antioxidant and Antihyaluronidase

2019 ◽  
Vol 24 (1) ◽  
pp. 52
Author(s):  
Tandiah Asan ◽  
I Nyoman Ehrich Lister ◽  
Edy Fachrial ◽  
Annisa Amalia ◽  
Wahyu Widowati ◽  
...  
Keyword(s):  
Free Radicals ◽  
Glycine Max ◽  
Aging Process ◽  
Skin Aging ◽  
Active Compounds ◽  
Black Soybean ◽  
Colorimetric Test ◽  
Soybean Extract ◽  
Glycine Max L ◽  
Antioxidant Potency

Black soybean (Glycine max (L.) Merr.) is a plant that is widely planted and consumed in Indonesia. In addition, black soybean has unique content of isoflavones, such as daidzein, which is one of the active compounds that have the effect of fighting free radicals and can inhibit the aging process. The purpose of this study is to analyze the antioxidant potency possessed by black soybean extract (BSE) and daidzeinin inhibiting aging of the skin. The method used is a colorimetric test. The type of antioxidant test used is H2O2 scavenging and inhibiting the activity of the hyaluronidase enzyme for antiaging. BSE has better effectiveness of H2O2 scavenging (IC50: 286.24±11.16 (µg/mL)) than daidzein compound (IC50: 366.16±2.54 (µg/mL)). In the inhibition of hyaluronidase enzyme, the daidzein has more effective activities (IC50: 95.80±3.98 (µg/mL)) compared to BSE (IC50: 152.56±13.98 (µg/mL)). The antioxidant and anti-aging activities possessed by BSE make it possible to be used as a cosmetic ingredient for skin aging therapy.

scholarly journals Formulation of Peel-off Gel Mask containing Mung Bean (Vigna radiata (L.) Wilczek) Extract

2019 ◽  
Vol 1 (2) ◽  
Author(s):  
Patihul Husni ◽  
Ella Masliana Dewi
Keyword(s):  
Antioxidant Activity ◽  
Vigna Radiata ◽  
Mung Bean ◽  
Aging Process ◽  
Methyl Cellulose ◽  
Skin Aging ◽  
Alpha Tocopherol ◽  
Potassium Sorbate ◽  
Film Forming ◽  
Ic50 Value

Mung bean (Vigna radiata (L.) Wilczek) is one of the plants that rich in antioxidant compound. Antioxidant is a compound that can inhibit the skin aging process because of photoaging. The aim of this study was to formulate peel-off gel mask containing mung bean (Vigna Radiata (L.) Wilczek) extract  using polyvinyl alcohol (PVA) as a base of mask and Hidroxy Prophyl Methyl Cellulose (HPMC) as a viscosity increasing agent and to determine the antioxidant activity of the peel-off gel mask. Antioxidant activity was tested using DPPH (1,1-diphenyl-2-pycrilhidrazil) assay. Mung bean was extracted by maceration method using ethanol 96%. The concentration of mung bean extract in the peel-off mask gel was 4% and various concentration of PVA were 5% (F1), 7.5% (F2), 10%(F3). The evaluations were organoleptic, pH, viscosity, drying and film forming, and gel spreadness. The study result showed that the organoleptic of the gel was brownish yellow with pH approximately 6, 196-513 cps in viscosity, 0.0646-0.0730 cm/g in gel spreadness and 27.6-54.5 second in drying and film forming. F3 containing mung bean extract 4%, PVA 10%, HPMC 2%, propylene glycol 15%, potassium sorbate 0,2%, olive oil 0,5%, alpha tocopherol 0,05 and aquadest ad 100% was the best formula with IC50 value was 85,2793 ppm and significantly different than F1 and F2 (p < 0.05).Keywords: peel-off gel mask, mung bean extract, Vigna radiata (L.) Wilczek, antioxidant

scholarly journals Aktivitas Antioksidan Fraksi Etil Asetat Dari Ekstrak Metanol Sargassum sp.

2019 ◽  
Vol 8 (1) ◽  
pp. 41-46
Author(s):  
Hafida Salma ◽  
Sri Sedjati Sedjati ◽  
Ali Ridlo
Keyword(s):  
Antioxidant Activity ◽  
Free Radicals ◽  
Vitamin C ◽  
Ethyl Acetate ◽  
Antioxidant Capacity ◽  
Total Antioxidant Capacity ◽  
Methanol Extract ◽  
Ethyl Acetate Fraction ◽  
Rotary Evaporator ◽  
Total Antioxidant

Sargassum sp. adalah salah satu jenis rumput laut paling banyak di Indonesia dan memiliki manfaat sebagai antioksidan, obat penyakit jantung, stroke, dll. Penelitian ini bertujuan untuk menentukan kandungan antioksidan fraksi etil asetat dari ekstrak metanol Sargassum sp. Sampel diambil dari Pantai Sundak, Gunung Kidul, Yogyakarta. Metode yang digunakan adalah eksploratif diskriptif. Sampel dikeringkan dalam suhu ruangan selama 7 hari dan dimaserasi dengan pelarut metanol, lalu dievaporasi dengan rotary evaporator. Ekstrak metanol di fraksinasi menggunakan etil asetat. Pengujian aktivitas antioksidan dilakukan dengan menggunakan 2 metode, yaitu metode penangkapan radikal bebas DPPH dan total kapasitas antioksidan fosfomolibdat. Vitamin C digunakan digunakan sebagai standar penangkapan radikal bebas DPPH dan total kapasitas antioksidan fosfomolibdat. Kadar total fenolat diuji menggunakan larutan Folin-Ciocalteu dengan standar asam galat. Hasil penelitian menunjukkan bahwa kadar total fenolat fraksi etil asetat Sargassum sp. sebesar 64,42 mg GAE/g sampel. Aktivitas antioksidan dengan metode penangkapan radikal bebas DPPH memiliki nilai IC50 1.289 ppm, vitamin C memiliki IC50 sebesar 122,71 ppm, sedangkan total kapasitas antioksidan adalah 39,52 mg AAE/g sampel. Kesimpulannya, yaitu kandungan aktivitas antioksidan fraksi etil asetat dari ekstrak metanol Sargassum sp. dengan metode penangkapan radikal bebas DPPH diduga sangat lemah dan kandungan aktivitas antioksidan dengan menggunakan metode fosfomolibdat diduga tinggi.  Sargassum sp. is one of the many types of seaweed in Indonesia and has benefits as an antioxidant, a drug for heart disease, stroke, etc. This study aims to determine the antioxidant content of ethyl acetate fraction of methanol extract of Sargassum sp. Samples were taken from Sundak, Gunung Kidul, Yogyakarta. The method used is descriptive explorative. Samples were dried at room temperature for 7 days and macerated with methanol, then evaporated at a rotary evaporator. The methanol extract fractionation using ethyl acetate. Testing the antioxidant activity using two methods, the method of catching free radicals DPPH and total antioxidant capacity fosfomolibdat. Vitamin C is used as a standard arrest DPPH free radical and total antioxidant capacity fosfomolibdat. Levels of total phenolics were tested using the Folin-Ciocalteu solution with gallic acid standard. The results showed that levels of total phenolic fraction of ethyl acetate Sargassum sp. amounting to 64,42 mg GAE/g sample. The antioxidant activity with catching free radicals DPPH methods have IC50 values 1.289 ppm, vitamin C has an IC50 of 122,71 ppm, while the total antioxidant capacity was 39,52 mg AAE/g sample. In conclusion, the content of the antioxidant activity of ethyl acetate fraction of methanol extract of Sargassum sp. with catching free radicals DPPH methods allegedly extremely weak and the content of the antioxidant activity using methods fosfomolibdat predictably high.

scholarly journals Atividade Antioxidante, Conteúdo de Fenólicos Totais, Carotenoides e Provitamina A em Extratos Vegetais do Cerrado Goiano

UNICIÊNCIAS ◽  
2018 ◽  
Vol 22 (1) ◽  
pp. 28
Author(s):  
Antonio Carlos Pereira de Menezes Filho ◽  
Josemar Gonçalves de Oliveira Filho ◽  
Marcela Christofoli ◽  
Castro Frederico de Sousa
Keyword(s):  
Antioxidant Activity ◽  
Free Radicals ◽  
Bioactive Compounds ◽  
Total Phenolic ◽  
Chemical Characteristics ◽  
Dpph Radical ◽  
Carotene Content ◽  
Phenolic Contents ◽  
Spectrophotometric Methods ◽  
Food Condiments

Diferentes grupos vegetais encontrados no bioma Cerrado vêm sendo amplamente estudados quanto as suas possíveis características químicas, apresentando dentre essas biocompostos de interesse para a indústria de alimentos, como na produção de barras de cereais nutritivas e em condimentos alimentares capazes de inibir ações de radicais livres causadores de patologias. No entanto, ainda pouco se sabe sobre a grande variedade destes compostos bioativos, que compõem as características químicas das espécies rasteiras, arbustivas e arbóreas, que coabitam nas mais diversas variantes deste bioma. A descoberta dos efeitos deletérios dos radicais livres sobre as células, agindo como causadores de doenças, impulsionando a busca por novos compostos bioativos na área de alimentos, que a cada ano cresce com a produção de novos produtos alimentícios, com características mais saudáveis, permitindo a prevenção e minimizando os danos oxidativos sobre as células. O presente trabalho teve como objetivo avaliar a atividade antioxidante pela captura do radical DPPH, conteúdos de fenólicos totais pelo reagente de Folin-Ciocalteau, carotenoides, provitamina A em equivalente de Retinol por métodos espectrofotométricos UV-Vis dos extratos etanólicos foliares das espécies botânicas: Byrsonima coccolobifolia (Kunth.), Dimorphandra mollis (Benth.), Hymenaea stignocarpa (Mart. ex Hayne), Solanum lycocarpum (St. Hil.) e Cardiopetalum calophyllum (Schlecht.). Os resultados demonstraram a presença de atividade antioxidante pelo método de sequestro do radical DPPH, expressivos conteúdos de fenólicos totais, teores de β-caroteno, presença de licopeno, exceto nas espécies Dimorphandra mollis e Hymenaea stignocarpa, presença de baixos teores de provitamina A.Palavras-chave: Folhas. DPPH. Licopeno.AbstractDifferent vegetable  groups found in the Cerrado biome have been widely studied as to their possible chemical characteristics, presenting among them biocomposites of interest for the food industry, as in the production of nutritious cereal bars and in food condiments capable of inhibiting actions of free radicals causing  pathologies. But little is known about the great variety of these bioactive compounds that make up the chemical characteristics of the shrub, shrub and tree species that cohabit in the most diverse variants of this biome. The discovery of the deleterious effects of free radicals on cells, acting as cause of disease, driving the search for new bioactive compounds in the area of food that grows every year with the production of new food products with healthier characteristics allowing prevention and minimizing the oxidative damage on  cells. The objective of the present work was to evaluate the antioxidant activity by capturing DPPH radical, total phenolic contents by Folin-Ciocalteau reagent, carotenoids, provitamin A in Retinol equivalent by UV-Vis spectrophotometric methods of the foliar ethanolic extracts of botanical species: Byrsonima Coccolobifolia (Kunth.), Dimorphandra mollis (Benth.), Hymenaea stignocarpa (Mart. ex Hayne), Solanum lycocarpum (St. Hil.) and Cardiopetalum calophyllum (Schlecht.). The results showed the presence of antioxidant activity by the DPPH radical sequestration method, expressive total phenolic content, β-carotene content, lycopene content, except for Dimorphandra mollis and Hymenaea stignocarpa species, with low levels of provitamin A.Keywords: Sheets. DPPH. Lycopene.

scholarly journals Redox Potential and Antioxidant Capacity of Bovine Bone Collagen Peptides towards Stable Free Radicals, and Bovine Meat Lipids and Proteins. Effect of Animal Age, Bone Anatomy and Proteases—A Step Forward towards Collagen-Rich Tissue Valorisation

Molecules ◽  
2020 ◽  
Vol 25 (22) ◽  
pp. 5422
Author(s):  
Laurent Aubry ◽  
Claude De-Oliveira-Ferreira ◽  
Véronique Santé-Lhoutellier ◽  
Vincenza Ferraro
Keyword(s):  
Antioxidant Activity ◽  
Free Radicals ◽  
Redox Potential ◽  
Antioxidant Capacity ◽  
Bone Age ◽  
Bone Collagen ◽  
Bovine Bone ◽  
Collagen Peptides ◽  
Stable Free Radicals ◽  
Effect Of Age

Collagen antioxidant peptides are being widely studied. However, no research has paid attention to biological parameters such as the age and anatomy of collagen-rich tissues, which can determine a change in tissue structure and composition, and then in bioactivity. Moreover, only few research works have studied and assessed peptides antioxidant activity on the food matrix. This work aimed to investigate the effect of bovine’s bone age and anatomy, and of six different enzymes, on the antioxidant activity of collagen peptides. Collagen was extracted from young and old bovine femur and tibia; six different enzymes were used for peptides’ release. The redox potential, the quenching of stable free radicals, and the antioxidant capacity on bovine meat lipids and proteins was evaluated, under heating from ambient temperature to 80 °C. Age and anatomy showed a significant effect; the influence of anatomy becomes most important with age. Each enzyme’s effectiveness toward age and anatomy was not the same. The greatest amount of peptides was released from young bones’ collagen hydrolysed with papain. The antioxidant activity was higher at higher temperatures, except for meat proteins. Assessing the effect of age and anatomy of collagen-rich tissues can promote a better application of collagen bioactive peptides.

scholarly journals Oxidative Stress, Skin Aging and Antioxidant Therapy / Oksidacioni Stres, Starenje Kože I Antioksidaciona Terapija

2014 ◽  
Vol 31 (4) ◽  
pp. 207-217 ◽  
Author(s):  
Dragana Stojiljković ◽  
Dušica Pavlović ◽  
Ivana Arsić
Keyword(s):  
Oxidative Stress ◽  
Free Radicals ◽  
Defense Mechanisms ◽  
Aging Process ◽  
Skin Diseases ◽  
Skin Aging ◽  
Common Mechanism ◽  
Dna Mutation ◽  
Endogenous Protection ◽  
The Impact

Summary Skin aging is a dynamic process that occurs due to chronological and photo-aging caused by the influence of external factors, especially ultraviolet-UV radiation. Cumulative oxidative stress, formation of free radicals and their adverse effects on biological systems in the skin cells is a common mechanism of both skin aging processes. The formed reactive oxygen species-ROS can lead to the oxidation of cell`s building molecules. Lipid peroxidation, membrane protein damage and DNA mutation may lead to many structural, functional and aesthetic changes in the skin and can also initiate development of many diseases. One of the skin defense mechanisms is antioxidant defense where enzymes and other antioxidant substances react directly with ROS, preventing them from reaching their biological target. Antioxidant substances have the ability to bind free radicals, caused by oxidative stress, and may have significance in prevention and/or therapy of various skin diseases, as well as in slowing the skin aging process. Topical application of antioxidants, such as vitamin C and E, coenzyme Q10 and polyphenolic compounds may provide the strengthening of the skin endogenous protection system and protection from harmful effects of ROS and oxidative damage to the skin. This article outlines the impact of oxidative stress on the chronological and photo- aging, its mechanism of action, the changes that occur on/in the skin during the aging process. Substances with antioxidant characteristics, which can be used in the prevention of chronological and/or treatment of photo-aging of the skin are shown.

scholarly journals Use of Yoghurt Enhanced with Volatile Plant Oils Encapsulated in Sodium Alginate to Increase the Human Body’s Immunity in the Present Fight Against Stress

2020 ◽  
Vol 17 (20) ◽  
pp. 7588
Author(s):  
Ovidiu Tița ◽  
Maria Adelina Constantinescu ◽  
Mihaela Adriana Tița ◽  
Cecilia Georgescu
Keyword(s):  
Antioxidant Activity ◽  
Free Radicals ◽  
Antioxidant Capacity ◽  
Sodium Alginate ◽  
Human Body ◽  
Raw Materials ◽  
Stress Factors ◽  
Cow Milk ◽  
Aromatic Plants ◽  
Volatile Oils

(1) Background: The COVID–19 pandemic and the imposition of strict but necessary measures to prevent the spread of the new coronavirus have been, and still are, major stress factors for adults, children, and adolescents. Stress harms human health as it creates free radicals in the human body. According to various recent studies, volatile oils from various aromatic plants have a high content of antioxidants and antimicrobial compounds. An external supply of antioxidants is required to destroy these free radicals. The main purpose of this paper is to create a yoghurt with high antioxidant capacity, using only raw materials from Romania; (2) Methods: The bioactive components used to enrich the cow milk yoghurt were extracted as volatile oils out of four aromatic plants: basil, mint, lavender and fennel. Initially, the compounds were extracted to determine the antioxidant capacity, and subsequently, the antioxidant activity of the yoghurt was determined. The 2,2-diphenyl-1-picrylhy-drazyl (DPPH) method was used to determine the antioxidant activity; (3) Results: The results show that cow milk yoghurt enhanced with volatile oils of basil, lavender, mint and fennel, encapsulated in sodium alginate has an antioxidant and antimicrobial effect as a staple food with multiple effects in increasing the body’s immunity. The antioxidant activity proved to be considerably higher than the control sample. The highest antioxidant activity was obtained on the first day of the analysis, decreasing onwards to measurements taken on days 10 and 20. The cow milk yoghurt enriched with volatile basil oil obtained the best results; (4) Conclusions: The paper shows that yoghurts with a high antioxidant capacity were obtained, using only raw materials from Romania. A healthy diet, compliance with safety conditions and finding appropriate and safe methods to increase the body’s immunity is a good alternative to a major transition through harder times, such as pandemics. The creation of food products that include natural antioxidant compounds combines both the current great possibility of developing food production in Romania and the prevention and reduction of the effects caused by pandemic stress in the human body.

scholarly journals THE SPF VALUE DETERMINATION OF EXTRACT AND SUNSCREEN LOTIO OF LIME RIND EXTRACT BY SPEKTROFOMETRI UV-VIS METHOD

2018 ◽  
Vol 1 (1) ◽  
pp. 16-19
Author(s):  
Sri Rejeki
Keyword(s):  
Antioxidant Activity ◽  
Free Radicals ◽  
Aging Process ◽  
Ethanol Extract ◽  
Fruit Skin ◽  
Main Factors ◽  
Value Determination ◽  
Lime Peel ◽  
Peel Extract

Lime (Citrus urantifolia) contains vitamin C and flavonoids which have antioxidant activity that can be used as a sunscreen. Antioxidants can repaire the effects of damage to human skin caused by free radicals which are the main factors in the aging process and damage to skin tissue. This  purposes of this study were to determine the SPF value of ethanol extract of lime peel and lotion of lime peel extract. Firstly, lime fruit skin was extracted by remaceration method using 70% ethanol and followed by determination or SPF values, lotion formulated and tested for SPF values with UV-VIS Spectrophotometry.The results showed that SPF values of the ethanol extract of lime peel in various concentrations 100ppm, 150ppm, 200ppm, 250ppm and 300ppm were respectively 4.77 ± 0.12; 10.17 ± 0.01; 27.05 ± 0.02; 50.33 ± 0.18; 80.54 ± 0.49. The result showed that SPF  value of lime ethanol extract  sunscreen lotio in 300ppm was 80.54 ± 0.49. Ethanol extract of lime fruit skin has the potential as a sunscreen.

scholarly journals Phytochemical screening and antioxidant activity of Epidendrum nocturnum

Bionatura ◽  
2021 ◽  
Vol 6 (1) ◽  
pp. 1486-1489
Author(s):  
Fernando Mencias ◽  
Telmo Salazar ◽  
Marco Cerna
Keyword(s):  
Antioxidant Activity ◽  
Free Radicals ◽  
Secondary Metabolites ◽  
Antioxidant Capacity ◽  
Average Concentration ◽  
Test Solution ◽  
Phytochemical Screening ◽  
Ethanolic Extracts

The objective of this study was to evaluate the antioxidant capacity of Epidendrum nocturnum using the DPPH technique to determine the capacity for scavenging free radicals, as well as to identify secondary metabolites in ethanolic extracts of the previously mentioned species by phytochemical screening, with analysis of alkaloids, flavonoids, saponins, tannins, and triterpenes. The results determined in the phytochemical screening that the secondary metabolites that were most present were flavonoids, tannins, and saponins; no alkaloids or triterpenes were found. In the analysis of antioxidant activity, Epidendrum nocturnun in the three extracts showed that with an average concentration of 3.50 ppm, it could inhibit 50% of the free radicals present in the test solution.

scholarly journals Polyphenolic Profile and Antioxidant Activity of Juglans regia L. Leaves and Husk Extracts

Forests ◽  
2019 ◽  
Vol 10 (11) ◽  
pp. 988 ◽  
Author(s):  
Anna Masek ◽  
Malgorzata Latos-Brozio ◽  
Ewa Chrzescijanska ◽  
Anna Podsedek
Keyword(s):  
Free Radicals ◽  
Antioxidant Capacity ◽  
High Performance ◽  
Juglans Regia ◽  
Lipid Fraction ◽  
Differential Pulse ◽  
Alpha Linolenic Acid ◽  
Spectrophotometric Methods ◽  
Research Technology ◽  
Natural Substances

The aim of this study is to characterize the antioxidant capacity and establish the profile of polyphenolic compounds in walnut extracts (different extracts prepared from walnut leaf and green husks). The correlation between bioingredients of the product tested and their ability to scavenge free radicals and reduce them by chelating various metal ions were examined. Research technology combining TG (thermogravimetry), FTIR (Fourier-transform infrared spectroscopy), high-performance liquid chromatography system (HPLC) with electrochemical methods (cyclic and differential pulse voltammetry) and spectrophotometric methods (ABTS, FRAP, and DPPH assays) was used to rate the potential oxidation-reduction components of walnut extracts. A high affinity for scavenging free radicals ABTS and DPPH was found for natural substances present in leaves and green husks. The walnut is beneficial to health as it contains alpha-linolenic acid in its lipid fraction and, as demonstrated in this study, its husks are rich in polyphenolics with high antioxidant capacity.

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