scholarly journals Phytochemical screening and antioxidant activity of Epidendrum nocturnum

Bionatura ◽  
2021 ◽  
Vol 6 (1) ◽  
pp. 1486-1489
Author(s):  
Fernando Mencias ◽  
Telmo Salazar ◽  
Marco Cerna
Keyword(s):  
Antioxidant Activity ◽  
Free Radicals ◽  
Secondary Metabolites ◽  
Antioxidant Capacity ◽  
Average Concentration ◽  
Test Solution ◽  
Phytochemical Screening ◽  
Ethanolic Extracts

The objective of this study was to evaluate the antioxidant capacity of Epidendrum nocturnum using the DPPH technique to determine the capacity for scavenging free radicals, as well as to identify secondary metabolites in ethanolic extracts of the previously mentioned species by phytochemical screening, with analysis of alkaloids, flavonoids, saponins, tannins, and triterpenes. The results determined in the phytochemical screening that the secondary metabolites that were most present were flavonoids, tannins, and saponins; no alkaloids or triterpenes were found. In the analysis of antioxidant activity, Epidendrum nocturnun in the three extracts showed that with an average concentration of 3.50 ppm, it could inhibit 50% of the free radicals present in the test solution.

EVALUATION OF ANTIOXIDANT ACTIVITY AND PHYTOCHEMICAL ANALYSIS OF HARDWIKIA BINATA (ROXB.)

2021 ◽  
pp. 256-258
Author(s):  
Varsha D. Hutke ◽  
Vaishali Rathod
Keyword(s):  
Antioxidant Activity ◽  
Free Radicals ◽  
Secondary Metabolites ◽  
Aqueous Extract ◽  
Ethanol Extract ◽  
Single Species ◽  
Antioxidant Property ◽  
Phytochemical Analysis ◽  
Phytochemical Screening ◽  
The Family

The genus Hardwickia of the family Caesalpiniaceae is represented by a single species Hardwickia binata Roxb. The present study deals with screening of secondary metabolites and antioxidant activity of different extracts of leaf and bark of Hardwickia binata. Phytochemical screening revealed the presence of various secondary metabolites viz. alkaloids, saponins, phenolics compound, tannins, phenols and steroids. Both leaf and bark extracts scavenged free radicals signicantly. Whereas aqueous extract of bark and ethanol extract of leaf exhibited good antioxidant property

UJI AKTIVITAS ANTIOKSIDAN FRAKSI N-HEKSAN DAUN AFRIKA (Vernonia amygdalina Del.) DENGAN METODE DPPH

2020 ◽  
Vol 5 (2) ◽  
pp. 250-257
Author(s):  
Nurul Fatimah ◽  
◽  
Reksi Sundu
Keyword(s):  
Oxidative Stress ◽  
Antioxidant Activity ◽  
Free Radicals ◽  
Ethanolic Extract ◽  
Ethanol Extract ◽  
Hexane Fraction ◽  
Phytochemical Screening ◽  
Vernonia Amygdalina ◽  
Tropical Africa ◽  
Ic50 Value

Free radicals and reactive species are widely believed to contribute to the development of several diseases by causing oxidative stress and eventually oxidative. Vernonia amygdalina (Astereacea) is a small shrub or tree between 1 and 5m high growing throughout tropical Africa. Plants are generally known as bitter leaves is well cultivated and is a general market for merchandise in several countries. The purpose of this study was to determine the antioxidant activity of hexane fraction from ethanol extract od Frican leaves (Vernonia amygdalina Del.). The method used in this study was the DPPH (1,1-Diphenil-2-Picrylhydrazyl) method. The result of phytochemical screening showed that ethanolic extract of African leaves contained a composition of secondary metabolites of alkaloids, flavonoids, tannins, steroids/triterpenoids and saponins. The antioxidant activity of the extract of n-hexane fraction was classified as very weak with an IC50 value of 317.98 ppm.

scholarly journals Antioxidant activity and total phenolic content in Caulerpa filiformis (Chlorophyta) from Sechura Bay and Paracas Bay, Peru

2020 ◽  
Vol 27 (1) ◽  
pp. 061-066 ◽  
Author(s):  
Joyce Mamani ◽  
Jorge Chávez ◽  
Eder Apumayta ◽  
Patricia Gil-Kodaka
Keyword(s):  
Antioxidant Activity ◽  
Secondary Metabolites ◽  
Methanolic Extract ◽  
Total Phenol ◽  
North Coast ◽  
Total Phenolic ◽  
Total Phenol Content ◽  
Phytochemical Screening ◽  
Phenol Content ◽  
The North

In Peru, Caulerpa filiformis is a marine algae listed as an invasive species. For years, its distribution has been considered to be in the north coast (Isla Lobos de Afuera and Piura) until a recent report of its distribution in the central coast (Ancash, Lima, and Ica). The present investigation aims to determine the main groups of secondary metabolites, total phenol content, and antioxidant activity of the methanolic extract of C. filiformis from Sechura Bay (Piura) and Paracas Bay (Ica). The main chemical groups were determined through phytochemical screening, the content of phenols by the Folin–Ciocalteu method, and antioxidant activity by the ABTS method (2,2-azinobis-[3-ethylbenzothiazoline-6-sulfonic acid]) and 2,2′-diphenyl-1-picrylhydrazyl (DPPH). The phytochemical screening of the methanolic extract of C. filiformis from Sechura Bay and Paracas Bay revealed the presence of carbohydrates, polyphenols, tannins, flavonoids, lipids, alkaloids, steroids, and triterpenes for both extracts. The total phenol content of the extract of C. filiformis from Sechura Bay (39.31 ± 0.39 mg of AGE/g extract) was significantly higher (p < 0.05) than that from Paracas Bay (18.78 ± 0.31 mg of AGE/g extract). In the ABTS and DPPH assays, the antioxidant capacity of the Sechura C. filiformis extract (IC50 = 3.49 ± 0.01 and 2.18 ± 0.02 mg/mL) was significantly higher (p < 0.05) than that of the Paracas C. filiformis extract (IC50 = 6.41 ± 0.02 and 2.42 ± 0.04 mg /mL). These findings suggest that the methanolic extract of C. filiformis is a source of secondary metabolites with an antioxidant potential.

scholarly journals Aktivitas Antioksidan Ekstrak Metanol Daun Alpukat Menggunakan Metode DPPH

2021 ◽  
Vol 11 (2) ◽  
pp. 104-110
Author(s):  
Alfin Surya ◽  
Zaiyar Nazir ◽  
Anggun Syazulfa
Keyword(s):  
Antioxidant Activity ◽  
Ascorbic Acid ◽  
Free Radicals ◽  
Methanol Extract ◽  
Natural Antioxidants ◽  
Positive Control ◽  
Phytochemical Screening ◽  
Bioactive Components ◽  
Ic50 Value ◽  
Ic50 Values

Free radicals are molecules that lose electrons in their outer orbitals so that the number of electrons becomes odd and unstable. Free radicals can damage various macromolecule cells including proteins, carbohydrates, fats. Antioxidants are compounds that can donate one or more electrons to free radicals. Avocado leaves contain high bioactive components that can be used as a source of natural antioxidants. Avocado leaves that have been dried, mashed and then shifted with methanol. The extracts were tested by phytochemical screening, including flavonpoid, phenolic, tannin and antioxidant activity using the DPPH (2,2-diphenyl-1-picrylhidrazyl) method. This method is based on the reduction of 2,2-diphenyl-1-picrylhydrazyl (DPPH) against free radicals which causes a change from purple to yellow 2,2-diphenyl-1-picrylhydrazyl) (DPPH). From the research, the IC50 values ​​in avocado leaves were 118.8056 µg / mL and 7,276 µg / mL on ascorbic acid as a positive control. From the IC50 value obtained, it is known that the methanol extract of avocado leaves has strong antioxidant activity to fight free radicals.

scholarly journals UJI AKTIVITAS ANTIOKSIDAN EKSTRAK ETANOL KULIT BATANG MAHONI (Swietenia mahagoni Jacq.) MENGGUNAKAN METODE DPPH (1,1 diphenyl-2-picrylhydrazyl)

PHARMACON ◽  
2019 ◽  
Vol 8 (3) ◽  
pp. 548
Author(s):  
Amanda T. M. Turangan ◽  
Defny S. Wewengkang ◽  
Adithya Yudistira
Keyword(s):  
Antioxidant Activity ◽  
Free Radicals ◽  
Vitamin C ◽  
Medicinal Plant ◽  
Positive Control ◽  
Antioxidant Compounds ◽  
Phytochemical Screening ◽  
Oxidation Reactions ◽  
Dpph Method ◽  
Swietenia Mahagoni

Antioxidant are compounds that are able to inactivate the development of the oxidation reactions of the molecules or neutralize free radicals. Mahogany bark (Swietenia mahagoni Jacq.) has properties as a medicinal plant. Through the phytochemical screening, the mahogany bark (Swietenia mahagoni Jacq.) contains antioxidant compounds such as alkaloids, saponins and phenolics. The aim of this study was to determine the presence of antioxsidant activity in the extract of mahogany bark (Swietenia mahagoni Jacq.). using the DPPH method with a concentration of 250 mg / L, 200 mg / L, 150 mg / L and 100 mg / L and Vitamin C p.a as a positive control. Each sample made three repetitions of the test. The test uses a UV-Vis Spectrophotometer. The result of the study showed that mahogany bark (Swietenia mahagoni Jacq.) showed antioxidant activity with the percentage of 21,50% at the concentration at 250 mg / LKeywords : Swietenia mahagoni Jacq., Antioxidant, Ethanol, DPPHABSTRAKAntioksidan ialah senyawa yang mampu menginaktivasi berkembangnya reaksi oksidasi molekul lain atau menetralisir radikal bebas. Kulit Batang Mahoni (Swietenia mahagoni Jacq.) memiliki khasiat sebagai tanaman obat. Melalui skrining fitokimia, Swietenia mahagoni Jacq. Mengandung senyawa antioksidan seperti alkaloid, saponin dan fenolik. Penelitian ini bertujuan untuk mengetahui adanya aktivitas antioksidan didalam ekstrak tanaman Kulit Batang Switenia mahagoni Jacq. menggunakan metode DPPH dengan konsentrasi 250 mg/L, 200 mg/L, 150 mg/L dan 100 mg/L dan Vitamin C p.a sebagai kontrol positif. Masing-masing sampel dibuat tiga kali pengulangan uji. Pengujian menggunakan alat Spektrofotometer UV-Vis. Hasil penelitian menunjukkan bahwa ekstrak Kulit Batang Swietenia mahagoni Jacq. memiliki aktivitas antioksidan dengan presentase sebesar 21,50% pada konsentrasi 250 mg/L Kata Kunci : Swietenia mahagoni Jacq., Antioksidan, Etanol, DPPH

scholarly journals ANTIOXIDANT ACTIVITY OF TARAXACUM OFFICINALE

InterConf ◽  
2021 ◽  
pp. 264-280
Author(s):  
Ala Fulga ◽  
Valeriana Pantea ◽  
Lilia Andronache ◽  
Olga Tagadiuc ◽  
Mihail Todiras ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Antioxidant Activity ◽  
Free Radicals ◽  
Hypochlorous Acid ◽  
Electron Flow ◽  
Taraxacum Officinale ◽  
Glutathione S Transferase ◽  
Hydrogen Atoms ◽  
Rheumatic Arthritis ◽  
Ethanolic Extracts

Background. The oxygen is the ultimate electron acceptor in the electron flow system that produces energy. Problems appears when the electron flow becomes uncoupled thus, generating free radicals. While the majority of processes require oxygen, the last is a highly reactive molecule that can damage cells by producing reactive oxygen species, such as hydroxyl radical, superoxide radical, hydrogen peroxide and hypochlorous acid, all together defined as triggers of oxidative stress. The last is involved in many types of pathologies, such as cancer, atherosclerosis and rheumatic arthritis. Antioxidants can stop formation of free radical by giving hydrogen atoms or scavenging them. The antioxidant can be defined any molecule capable of preventing or blocking oxidation of lipids, proteins and nucleic acids. The reduced glutathione (GSH), as well enzymes, glutathione peroxidase (GPO), glutathione reductase (GR) and glutathione S-transferase (GST) represent antioxidants which prevent the formation of new free radicals, converting them into less harmful molecules. The present study was conducted to assess the antioxidant activity of Taraxacum officinale (TO) ethanolic extracts with different bioactive contents. This experiment was conducted with extracts prepared from dandelion (Taraxacum officinale F. H. Wigg) leaves. A series (10%, 20%, 25%, 40%, 50% and 80%) of ethanolic extracts were made. The influence of TO extracts on RBC’s GPO, GR, GST, glutathione (GSSG, GSH and total-GSH) and thiols (native and total) was evaluated by measuring absorbance spectrophotometrically. Conclusions. Taraxacum officinale ethanolic extracts exhibit a strong antioxidant activity. This plant is capable of preventing damage of cells during oxidative stress process, by using different targets of glutathione system. But, this activity depends of ethanol concentrations, maybe due to different bioactive content.

scholarly journals Secondary Metabolites and Bioactivity of Hyophila involuta (Hook) Jaeg.

2015 ◽  
Vol 7 (4) ◽  
pp. 456-459
Author(s):  
Amos M. MAKINDE ◽  
Eunice A. FAJUYIGBE ◽  
Musibau O. ISA
Keyword(s):  
Staphylococcus Aureus ◽  
Secondary Metabolites ◽  
Aspergillus Flavus ◽  
Cardiac Glycosides ◽  
Therapeutic Potential ◽  
Ethanolic Extract ◽  
Acetone Extract ◽  
Significant Activity ◽  
Phytochemical Screening ◽  
Ethanolic Extracts

The phytochemical screening of Hyophila involuta collected from the Biological Garden of the Obafemi Awolowo University, Ile-Ife, Nigeria, was carried out to investigate the presence or absence of some secondary metabolites and its antibiotic potentials, using different extracts (with acetone and ethanol) on selected organisms. The extracts obtained were screened for the presence of secondary metabolites like alkaloids, anthraquinones, cardiac glycosides, flavonoids, phlobatanins, saponins, steroids, tannins, triterpenes and xanthoproteins. Antimicrobial activity of the extracts was carried out on Escherichia coli, Staphylococcus aureus, Aspergillus flavus and Candida albicans. Only acetone extract tested positive for the presence of flavonoids, while alkaloids and cardiac glycosides were detected present in both the acetone and ethanolic extracts. Flavonoids were detected present only in the acetone extract. Saponins were detected present only in the ethanolic extract. The extracts (acetone and ethanolic) showed significant activity on Staphylococcus aureus and Aspergillus flavus. The results obtained from this study indicated that H. involuta has medicinally important compounds, having therapeutic potential from which effective antimicrobial medicine can be sourced.

scholarly journals PHYTOCHEMICAL STUDY AND IN VITRO ANTIOXIDANT ACTIVITIES OF HAMMADA SCOPARIA EXTRACTS FROM SOUTHEASTERN ALGERIA

2018 ◽  
Vol 11 (10) ◽  
pp. 187
Author(s):  
Benkherara Salah ◽  
Bordjiba O
Keyword(s):  
Antioxidant Activity ◽  
Secondary Metabolites ◽  
Condensed Tannins ◽  
Aerial Part ◽  
Reducing Power ◽  
Total Flavonoids ◽  
Total Phenols ◽  
Phytochemical Screening ◽  
Trolox Equivalent

Objective: This study was carried out to determine the phytochemical constituents and to evaluate the antioxidant potential of the aerial part extracts of Hammada scoparia (Pomel) Iljin to validate the medicinal potential of this Algerian plant species.Methods: Crude extracts were prepared by cold maceration with absolute methanol and distilled water. Preliminary phytochemical screening is carried out to detect the presence of the major secondary metabolites using qualitative characterization methods. Quantitative estimation of total phenols, total flavonoids, flavanols, flavonols, and condensed tannins contents is performed using gallic acid, rutin, catechin, and quercetin as standards. In vitro antioxidant activity was evaluated by the free radical scavenging activity by 2,2-diphenyl-1-picrylhydrazyl (DPPH), trolox equivalent antioxidant capacity by scavenging of 2,2′-azinobis-(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) radical cation assay, and the ferric reducing power assay (ferric reducing antioxidant power).Results: Phytochemical screening showed the presence of alkaloids, flavonoids, tannins, terpenes and sterols, saponins, and anthocyanins. Total phenols were present more in aqueous crude extract (ACE) with 336.756±0.855 mg gallic acid equivalent/g DM. Total flavonoids and flavonols were more abundant in methanolic crude extract (MCE) than ACE. However, condensed tannins and flavanols were present less in MCE with only 0.958±0.052 and 4.547±0.055 mg CE/g DM, respectively. The ACE of this plant species had greater antioxidant activity than the other extract by DPPH and ABTS assays (35.823±0.129 and 51.323±0.394 mg trolox equivalent/g DM). The better ferric reducing power (2060.535±2.566 mM Fe+2/g DM) was also recorded with the same extract.Conclusion: ACE of aerial part of H. scoparia (Pomel) Iljin showed a high amount of secondary metabolites. The obtained results confirmed that the extracting solvent influenced the antioxidant property estimations of this plant. Hence, the ACE can be further exploited further for in vitro and in vivo research work.

scholarly journals Antioxidant Capacity and Phenolic Content of four Myrtaceae Plants of the South of Brazil

2011 ◽  
Vol 6 (7) ◽  
pp. 1934578X1100600 ◽  
Author(s):  
Marcos José Salvador ◽  
Caroline C. de Lourenço ◽  
Nathalia Luiza Andreazza ◽  
Aislan C.R.F. Pascoal ◽  
Maria Élida Alves Stefanello
Keyword(s):  
Antioxidant Activity ◽  
Antioxidant Capacity ◽  
Transfer Reaction ◽  
Phenolic Content ◽  
Hydrogen Atom Transfer ◽  
Preliminary Evaluation ◽  
Dpph Assay ◽  
The South ◽  
Ethanolic Extracts ◽  
South Of Brazil

Antioxidant compounds can be useful to prevent several degenerative diseases or as preservative in food and toiletries. Species of the Myrtaceae family are able to accumulate phenolic substances and those are closely related to the antioxidant activity due to their capacity to scavenge free radicals, protect against lipid peroxidation and quench reactive oxygen species. These facts prompted us to investigate the antioxidant capacity of the ethanolic extracts of the leaves of four Myrtaceae plants collected of the south of Brazil: Eugenia chlorophylla O. Berg., Eugenia pyriformis Cambess, Myrcia laruotteana Cambess and Myrcia obtecta (Berg) Kiacrsk. The antioxidant potential was performed using the DPPH (a single electron transfer reaction based assay) and ORAC (Oxygen Radical Absorbance Capacity, a hydrogen atom transfer reaction based assay) assays. Moreover, the total soluble phenolic content was also measured using the Folin-Ciocalteu reagent. A preliminary evaluation of the ethanolic extracts of these Myrtaceae plants revealed high levels of phenolic compounds (343.7-429.3 mg GAE) as well as high antioxidant activity according to both methods (1338 a 3785 μmol of TE/g of extract in ORAC and SC50 in the range of 1.70 and 33.7 μg/mL in the DPPH). The highest antioxidant activity obtained by DPPH assay was exhibited by ethanol extract of the leaves of E. pyriformis (1.70 μg/mL), followed by extracts of M. laruotteana (3.38 μg/mL) and M. obtecta (6.66 μg/mL). In comparison with controls, in the DPPH assay, the extract of E. pyriformis was more active than trolox (SC50 = 2.55 μg/mL), while the extracts of M. laruotteana and M. obtecta were more actives than quercetin (SC50 = 7.80 μg/mL). In the ORAC assay, all species also show good antioxidant capacity (>1000 μmol of TE/g). Initial HPLC-UV/DAD and ESIMS confirmed the presence of phenolic acids constituents in the ethanol extracts. The results indicate the presence of compounds possessing promising antioxidant/free-radical scavenging activity in the analyzed extracts of Myrcia and Eugenia plants of the south of Brazil.

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