scholarly journals Alleviation of Osteoarthritis-Induced Pain and Motor Deficits in Rats by a Novel Device for the Intramuscular Insertion of Cog Polydioxanone Filament

2021 ◽  
Vol 11 (22) ◽  
pp. 10534
Author(s):  
Kilyong Lee ◽  
Geung Gyu Gang ◽  
Yun Gyu Kang ◽  
Sung Sam Jung ◽  
Hee-Gon Park ◽  
...  
Keyword(s):  
Motor Impairment ◽  
Rectus Femoris ◽  
Motor Deficits ◽  
Mobility Limitations ◽  
Necrosis Factor Alpha ◽  
Cross Sectional ◽  
Knee Oa ◽  
Novel Device ◽  
Structural Improvement ◽  
Factor Alpha

The importance of skeletal muscles in the development of osteoarthritis (OA) is known. However, in OA, strengthening the muscle is arduous process. This study investigated the effects of muscle enhancement and support therapy (MEST), a novel device for the intramuscular insertion of cog polydioxanone filament intended to hold and stimulate surrounding muscles, on OA-induced symptoms. In our results, the MEST attenuated OA-induced pain and mobility limitations, as evidenced by increases in withdrawal thresholds, rearing duration and travelled distance in an open cage, and fall latency from rotarod. It further restored atrophic rectus femoris muscle (RFM) in OA animals by increasing mass, decreasing nucleus density, and increasing the cross-sectional area of muscle fibers. Decreased collagen and insulin-like growth factor 1 levels in OA animals were restored without affecting the interleukin-6 and tumor necrosis factor-alpha levels in RFM. No evidence of structural improvement in the knee was observed via computed tomography after MEST. These results suggest that MEST in the quadriceps is effective for relieving pain and motor impairment in knee OA animals by restoring atrophic muscles, providing a novel therapeutic strategy for OA symptom management.

scholarly journals Potential Influence of Menstrual Status and Sex Hormones on Female Severe Acute Respiratory Syndrome Coronavirus 2 Infection: A Cross-sectional Multicenter Study in Wuhan, China

2020 ◽  
Author(s):  
Ting Ding ◽  
Jinjin Zhang ◽  
Tian Wang ◽  
Pengfei Cui ◽  
Zhe Chen ◽  
...  
Keyword(s):  
Severe Case ◽  
Potential Influence ◽  
Necrosis Factor Alpha ◽  
Cross Sectional ◽  
Female Patients ◽  
Menstrual Status ◽  
Case Rate ◽  
Factor Alpha ◽  
Lower Morbidity ◽  
Necrosis Factor

Abstract Background Recent studies have indicated that females with coronavirus disease 2019 (COVID-19) have a lower morbidity, severe case rate, and mortality and better outcome than those of male individuals. However, the reasons remained to be addressed. Methods To find the factors that potentially protect females from COVID-19, we recruited all confirmed patients hospitalized at 3 branches of Tongji Hospital (N = 1902), and analyzed the correlation between menstrual status (n = 509, including 68 from Mobile Cabin Hospital), female hormones (n = 78), and cytokines related to immunity and inflammation (n = 263), and the severity/clinical outcomes in female patients <60 years of age. Results Nonmenopausal female patients had milder severity and better outcome compared with age-matched men (P < .01 for both). Menopausal patients had longer hospitalization times than nonmenopausal patients (hazard ratio [HR], 1.91 [95% confidence interval {CI}, 1.06–3.46]; P = .033). Both anti-Müllerian hormone (AMH) and estradiol (E2) showed a negative correlation with severity of infection (adjusted HR, 0.146 [95% CI, .026–.824], P = .029 and 0.304 [95% CI, .092–1.001], P = .05, respectively). E2 levels were negatively correlated with interleukin (IL) 2R, IL-6, IL-8, and tumor necrosis factor alpha in the luteal phase (P = .033, P = .048, P = .054, and P = .023) and C3 in the follicular phase (P = .030). Conclusions Menopause is an independent risk factor for female COVID-19 patients. AMH and E2 are potential protective factors, negatively correlated with COVID-19 severity, among which E2 is attributed to its regulation of cytokines related to immunity and inflammation.

scholarly journals Correlation between tumor necrosis factor-alpha and septic shock in children

2013 ◽  
Vol 53 (1) ◽  
pp. 1 ◽  
Author(s):  
Khrisanti Dinata ◽  
Ari L. Runtunuwu ◽  
Jose M. Mandei ◽  
Julius H. Lolombulan
Keyword(s):  
Septic Shock ◽  
Tumor Necrosis Factor ◽  
Tumor Necrosis Factor Alpha ◽  
Tumor Necrosis ◽  
Strong Positive Correlation ◽  
Necrosis Factor Alpha ◽  
Cross Sectional ◽  
Positive Correlation ◽  
Factor Alpha ◽  
Necrosis Factor

Background The crucial role cytokines play in the pathophysiologyof sepsis is widely accepted. Infection stimulates the productionof cytokines in various cell types. Tumor necrosis factor-alpha(TNF-a) is one of the most extensively investigated cytokines inexperimental and clinical sepsis. Tumor necrosis factor-alpha hasbeen shown to mediate lethality in experimental sepsis.Objective To evaluate for a possible correlation between TNF-alevel and septic shock in children.Methods This cross-sectional study was conducted in Manadofrom June to September 2011. A total of 40 patients with arecent diagnosis of sepsis or septic shock were included. Plasmaspecimens were collected from subjects for measurement ofTNF-a concentration. Logistic regression analysis was used toassess the correlation between TNF-a level and sepsis, as well asthe probability of shock in children with sepsis, with P<0.05 asstatistically significant.Results There was a strong positive correlation betweenTNF-a level and the probability of shock in children with sepsis(regression coefficient = 0. 78, P = 0.002).Conclusions There is a strong positive correlation betweenTNF-a level with the probability of shock in children with sepsis.Higher plasma level ofTNF-a is associated with higher probabilityof septic shock.

scholarly journals Synergistic effects of hip/knee osteoarthritis and comorbidities on mobility and self-care limitations among older adults: Cross-sectional analysis of the Oxford pain, Activity and Lifestyle study

2020 ◽  
Vol 10 ◽  
pp. 2235042X2097452
Author(s):  
Philippa JA Nicolson ◽  
Esther Williamson ◽  
Hopin Lee ◽  
Alana Morris ◽  
Angela Garrett ◽  
...  
Keyword(s):  
Older Adults ◽  
Depressive Symptoms ◽  
Knee Osteoarthritis ◽  
Synergistic Effects ◽  
Self Care ◽  
Postal Questionnaire ◽  
Community Dwelling ◽  
Mobility Limitations ◽  
Cross Sectional ◽  
Knee Oa

Objective: To estimate synergistic effects of hip/knee osteoarthritis (OA) and comorbidities on mobility or self-care limitations among older adults. Methods: We used baseline, cross-sectional data from the Oxford Pain, Activity and Lifestyle (OPAL) study. Participants were community-dwelling adults aged 65 years or older who completed a postal questionnaire. Participants reported demographic information, hip/knee OA, comorbidities and mobility and self-care limitations. We used modified Poisson regression models to estimate the independent and combined relative risks (RR) of mobility or self-care limitations, the relative excess risk due to interaction (RERI) between hip/knee OA and comorbidities, attributable proportion of the risk due to the interaction and the ratio of the combined effect and the sum of the individual effects, known as the synergy index. Results: Of the 4,972 participants included, 1,532 (30.8%) had hip/knee OA, and of them 42.9% reported mobility limitations and 8.4% reported self-care limitations. Synergistic effects impacting self-care limitations were observed between hip/knee OA and anxiety (RR: 3.09, 95% Confidence Interval (CI): 2.00 to 4.78; RERI: 0.93, 95% CI: 0.01 to 1.90), and between hip/knee OA and depressive symptoms (RR: 2.71, 95% CI: 1.75 to 4.20; RERI: 0.58, 95% CI: 0.03 to 1.48). The portion of the total RR attributable to this synergism was 30% and 22% respectively. Conclusions: This study demonstrates that synergism between hip/knee OA and anxiety or depressive symptoms contribute to self-care limitations. These findings highlight the importance of assessing and addressing anxiety or depressive symptoms when managing older adults with hip/knee OA to minimize self-care limitations.

scholarly journals HYPEROXALURIA AND BIOMARKERS OF MUCOSAL IMMUNITY IN PATIENTS WITH RECURRENT PYELONEPHRITIS

2014 ◽  
pp. 42-48
Author(s):  
M. Kolesnyk ◽  
N. Stashevska ◽  
N. Stepanova ◽  
V. Dryyanskaya ◽  
A. Rudenko ◽  
...  
Keyword(s):  
Mucosal Immunity ◽  
Immunoglobulin A ◽  
Cross Sectional Study ◽  
Necrosis Factor Alpha ◽  
Cross Sectional ◽  
Oxalate Excretion ◽  
Group I ◽  
Intestinal Dysbiosis ◽  
Factor Alpha ◽  
Necrosis Factor

Summary: The aim of our study was to compare the performance of mucosal immunity in urine and saliva of patients with chronic recurrent pyelonephritis subject to availability of hyperoxaluria. Material and methods. To observational cross–sectional study included 40 women with chronic recurrent pyelonephritis, aged 21 to 48 years (31.6±7.7). Depending on the availability hyperoxaluria (oxalate excretion in the urine than 0.45 mmol per day) patients were divided into II Groups: for I (n=29) included women with hyperoxaluria, to II (n=11) – includes patients with normal excretion oxalate (7.2±2.4 vs 43.8±5.2; p <0.001). State of mucosal immunity was assessed by determining the content of lysozyme, lactoferrin, secretory immunoglobulin A (sIg A) and tumor necrosis factor alpha (TNF– a) in urine samples and content sIg A and class antibodies sIg A to lipopolysaccharide (LPS) of gram–negative bacteria (anti–LPS–sIgA) in saliva. Results. We have identified significantly higher levels of sIg A and anti–LPS–sIgA in the saliva ofpatients with recurrent pyelonephritis with hyperoxaluria (298±104 vs 150.1±79.3 mg/1, p<0.001) and (0.353±0.16 vs 0.211±0.09, p<0.001), respectively. In the urine ofwomen of group I we havefound a statistically significant increase in the content of TNF– a 44 [16.2–130.5] vs 21 [14.2–3.45] pg/ml (p=0.04) and lysozyme 14.0[2.5– 36.5]vs 1.45[0.12–7.5]ng/ml (p=0.002). All the studied parameters (anti–LPS–sIg A in saliva and sIg A, lactofer– rin and lysozyme urine) had a direct correlation with the level of daily oxalate excretion.   Conclusions. Overproduction of indicators of mucosal immunity may be explained by the formation of intestinal dysbiosis under the influence of continuous antibiotic therapy. The imbalance of intestinal microflora, in turn, leads to the formation of hyperoxaluria and increases the production of antibodies to LPS, sIg A, lactoferrin and lysozyme.  

Variables Associated with the Assessment of Systemic Tumor Necrosis Factor Alpha Levels during Hemodialysis

1992 ◽  
Vol 15 (11) ◽  
pp. 653-657 ◽  
Author(s):  
A. Jörres ◽  
P. Froese ◽  
C. Fischer ◽  
H. Safak ◽  
G.M. Gahl ◽  
...  
Keyword(s):  
Tumor Necrosis Factor ◽  
Tumor Necrosis Factor Alpha ◽  
Whole Blood ◽  
Tumor Necrosis ◽  
Individual Variability ◽  
Necrosis Factor Alpha ◽  
Cross Sectional ◽  
Factor Alpha ◽  
Necrosis Factor

Conflicting results have been published concerning the systemic induction of the cytokine tumor necrosis factor alpha (TNFα) during hemodialysis (HD). We therefore evaluated in vitro TNFα production in whole blood as well as in vivo variability of TNFα levels in patients on long-term HD. Whole blood was incubated at room temperature (RT) with or without exogenously added endotoxin (ET), and plasma-TNFα was measured after 5, 30, 120, 240, and 960 min by specific enzyme immunoassay. Additionally, plasma-TNFα before and after 120 and 240 min HD was studied longitudinally once a week over a period of 4 weeks in 36 patients on Cuprophan® (CU, n=23) or polysulfone-F60 (PSu, n=13) HD. Mean plasma TNFα levels in vitro rose from (mean) 8 pg/ml after 5 min to 12 pg/ml (120′) and 32 pg/ml (960′) even without ET addition, and to 18 pg/ml (after 120′) and 88 pg/ml (after 960′) when 0.1 μg/ml ET were added. Pre-dialytic as well as intradialytic TNFα levels in patients showed high intra-individual variability. A substantial (> 100%) increase in plasma TNFα was observed during only 14 out of 84 treatments with CU and 20 out of 47 with PSu, however, the increase in TNFα was not statistically significant in either group. We conclude that the sampling procedure, if not carefully standardized, is a potential source of artifacts with regard to “systemic” TNFα levels. The high intra and inter-individual variability of plasma TNFα suggests that results of cross-sectional studies are questionable.

scholarly journals TINGKAT KECUKUPAN VITAMIN A, SENG DAN ZAT BESI SERTA FREKUENSI INFEKSI PADA BALITA STUNTING DAN NON STUNTING

2018 ◽  
Vol 13 (2) ◽  
pp. 168 ◽  
Author(s):  
Nabilla Siti Hawa Fatimah ◽  
Bambang Wirjatmadi
Keyword(s):  
Growth Hormone ◽  
Vitamin A ◽  
Interleukin 1 ◽  
Iron Intake ◽  
Necrosis Factor Alpha ◽  
Cross Sectional ◽  
Tnf Α ◽  
Factor Alpha ◽  
Cross Sectional Design ◽  
Necrosis Factor

Stunting is a disturbance in linear growth. Defi ciency of micronutrients such as vitamin A, zinc, and iron may disturb secretion of growth hormone. Infection may improve Tumor Necrosis Factor Alpha (TNF-α) and Interleukin 1 (IL-1)that pressing secretion of growth hormone. The purpose of this study is to determine difference adequacy levels of vitamin A, zinc, iron and frequency infection in stunting and non stunting children under fi ve at Puskesmas BulakBanteng Surabaya. The study was conducted from January to may 2017 with cross sectional design. Thirty-eight children (19 stunting and 19 non stunting) were selected from random sampling. Mann Whitney test was conducted to analyze the differences between variabel in stunting and non stunting. Variable that were signifi cantly different between two groups were vitamin A (p=0.002), zinc (p=0.003) and iron adequacy level (p=0.030). There were no signifi cant differences in frequency of diarrhea and acute respiratory infection among both groups. Vitamin A, zinc, and iron intake should be fullfi lled in order to prevent stunting.

scholarly journals RESPON ADAPTASI MOLEKULER IMUNITAS TUBUH PENDUDUK YANG BERADA DI LINGKUNGAN TERPAPAR POLUSI UDARA

2018 ◽  
Vol 9 (2) ◽  
Author(s):  
Mohammad Zulkarnain ◽  
Rostika Flora
Keyword(s):  
Tumor Necrosis Factor ◽  
Tumor Necrosis Factor Alpha ◽  
Interleukin 6 ◽  
Tumor Necrosis ◽  
Necrosis Factor Alpha ◽  
Cross Sectional ◽  
Tnf Α ◽  
Factor Alpha ◽  
Necrosis Factor

Latar Belakang: Perindustrian di berbagai wilayah dunia telah berpengaruh terhadap polusi ataupencemaran udara. Paparan polusi udara secara terus-menerus dapat mengakibatkan penurunan sistem imun.Tujuan penelitian ini adalah untuk mengetahui respon molekuler imunitas tubuh penduduk yang berada dilingkungan terpapar polusi udara.Metode: Jenis penelitian ini adalah observasional analitik dengan rancangan cross sectional. Populasipenelitian ini adalah seluruh masyarakat yang tinggal di sekitar Pabrik Karet Gandus dan TPA sampahSukawinatan Palembang yang berjumlah 60 orang yang memenuhi kriteria inklusi. Pemeriksaan kadar TNF-α dan IL-6 menggunakan teknik ELISA Human kit, pengukuran kadar H2S dilakukan pada jarak 250 meter,dengan metode biru metilen.Hasil Penelitian: Kadar H2S di sekitar TPA Sampah Sukawinatan (0,428 ppm) lebih tinggi dibandingkankadar H2S disekitar Pabrik Karet Gandus (0,332 ppm). Tidak terdapat perbedaan yang bermakna rerata kadarTNF-α (p=0,701) dan rerata kadar IL-6(p=0,618) antara kedua lokasi. Nilai korelasi karakteristik respondendengan kadar TNF-α dan kadar IL-6 di dua lokasi penelitian sangat lemah dan tidak bermakna secarastatistik. Nilai korelasi antara dengan IL-6 sangat lemah dan tidak bermakna secara statistik di sekitar PabrikKaret Gandus (r= 0,284; p=0,128) dan di sekitar TPA sampah Sukawinatan (r=-0,258;p=0,169).Kesimpulan: Meskipun kadar H2S di sekitar TPA Sampah Sukawinatan lebih tinggi, diharapkan pendudukyang berada disekitar Pabrik karet dan TPA sampah menggunakan alat pelindung diri seperti masker saatberada diluar rumah dan menjaga asupan nutrisi dengan baik agar kekebalan tubuh terjaga.Kata kunci: Hidrogen Sulfida, tumor necrosis factor-alpha, interleukin-6.

scholarly journals Oral administration of EP4-selective agonist KAG-308 suppresses mouse knee osteoarthritis development through reduction of chondrocyte hypertrophy and TNF secretion

2019 ◽  
Vol 9 (1) ◽  
Author(s):  
Yasutaka Murahashi ◽  
Fumiko Yano ◽  
Ryota Chijimatsu ◽  
Hideki Nakamoto ◽  
Yuji Maenohara ◽  
...  
Keyword(s):  
Oral Administration ◽  
Cartilage Degeneration ◽  
Necrosis Factor Alpha ◽  
Chondrocyte Hypertrophy ◽  
Knee Oa ◽  
Selective Agonist ◽  
Histone Deacetylase 4 ◽  
Factor Alpha ◽  
Disease Modifying Treatment ◽  
Tnf Secretion

AbstractOsteoarthritis (OA) is one of the world’s most common degenerative diseases, but there is no disease-modifying treatment available. Previous studies have shown that prostaglandin E2 (PGE2) and PGE2 receptor 4 (EP4) are involved in OA pathogenesis; however, their roles are not fully understood. Here, we examined the efficacy of oral administration of KAG-308, an EP4-selective agonist, in surgically induced mouse knee OA. Cartilage degeneration and synovitis were significantly inhibited by the KAG-308 treatment. Chondrocyte hypertrophy and expression of tumor necrosis factor alpha (TNF) and matrix metalloproteinase 13 (Mmp13) in the synovium were suppressed in the KAG-308-treated mice. In cultured chondrocytes, hypertrophic differentiation was inhibited by KAG-308 and intranuclear translocation of histone deacetylase 4 (Hdac4) was enhanced. In cultured synoviocytes, lipopolysaccharide (LPS)-induced expression of TNF and Mmp13 was also suppressed by KAG-308. KAG-308 was detected in the synovium and cartilage of orally treated mice. TNF secretion from the synovia of KAG-308-treated mice was significantly lower than control mice. Thus, we conclude that oral administration of KAG-308 suppresses OA development through suppression of chondrocyte hypertrophy and synovitis. KAG-308 may be a potent candidate for OA drug development.

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