scholarly journals Effects of the Origin and Deacetylation Degree of Chitosan on Properties of Its Coatings on Titanium

Coatings ◽  
2020 ◽  
Vol 10 (2) ◽  
pp. 99
Author(s):  
Milena Supernak-Marczewska ◽  
Andrzej Zielinski
Keyword(s):  
Titanium Surface ◽  
Surface Oxidation ◽  
Chitosan Solution ◽  
Biological Properties ◽  
Contact Angles ◽  
Biological Behavior ◽  
Water Contact ◽  
Deacetylation Degree ◽  
Oxidation In Air

The properties of chitosan coatings on titanium surfaces may be influenced by a variety of factors, including their chemical characteristics and the deposition method. The aim of this research was to determine the influence of a chitosan’s origin (a type of shrimp) and deacetylation degree (DD), when deposited on a very smooth titanium surface, on adhesion and biological behavior. The tests were performed using chitosan of a degree of 87% or 84% of deacetylation and that originated from armor crabs or shrimp armor. The technology of fabrication of chitosan coatings was by surface polishing to a smooth surface, oxidation in air, and immersion in a chitosan solution. The surface topographies were analyzed with an atomic force microscope and their water contact angles were measured by a falling drop method with a goniometer. The bioactivity tests were done in in vitro on osteogenic cells, type MC3T3-E1, with a biological microscope. The abrasion of the coatings was examined using a nano tribotester. The obtained results revealed that the adhesion of the coatings onto a smooth, oxidized titanium surface is appropriate as they remain sufficiently adjacent to the surface after wear tests. The source of chitin has a significant influence on biological properties, and the deacetylation degree is much less critical. The performed tests demonstrated the crucial role that the source of chitosan and the applicability of the applied surface treatment play in the preparation of chitosan coatings.

Surface Modification of PEOT/PBT Membrane with Silk Fibroin Anchoring and its Potential Application in Artificial Salivary Gland Construct

2012 ◽  
Vol 538-541 ◽  
pp. 52-59
Author(s):  
Jie Zhu ◽  
Ming Shi Li ◽  
Li Qun Wang ◽  
Xiao Lin Zhu
Keyword(s):  
Salivary Gland ◽  
Silk Fibroin ◽  
Potential Application ◽  
Contact Angles ◽  
Water Contact ◽  
Polar Groups ◽  
Poly Ethylene Oxide ◽  
Poly Ethylene ◽  
Surface Modified

We reported the preparation of surface modified poly (ethylene oxide terephthalate) - poly (butylene terephthalate) membrane by the method of silk fibroin anchoring, namely SF/(PEOT/PBT). Its surface properties were characterized by contact angles and XPS and the biocompatibility of the composite membrane was further evaluated by human salivary epithelial cells (HSG cells) growth in vitro. Results revealed that SF/(PEOT/PBT) possessed the low water contact angle (48.0±3.0°) and immobilized a great amount of fibroin (fibroin surface coverage: 26.39 wt%), which attributed to the formation of polar groups such as hydrosulfide group, sulfonic group, carboxyl and carbonyl ones in the process of SO2 plasma treatment. HSG cells growth in vitro indicated that the silk fibroin anchoring could significantly enhance the biocompatibility of PEOT/PBT membrane, which suggested the potential application of fibroin anchoring PEOT/PBT for clinical HSG cells transplantation in the artificial salivary gland construct.

scholarly journals Polycaprolactone-Chitin Nanofibrous Mats as Potential Scaffolds for Tissue Engineering

2012 ◽  
Vol 2012 ◽  
pp. 1-9 ◽  
Author(s):  
Min Sup Kim ◽  
Sang Jun Park ◽  
Bon Kang Gu ◽  
Chun-Ho Kim
Keyword(s):  
Electron Microscopy ◽  
Tissue Engineering ◽  
Contact Angles ◽  
Dermal Fibroblasts ◽  
Human Dermal Fibroblasts ◽  
Water Contact ◽  
Mean Diameter ◽  
Poly Caprolactone ◽  
Scanning Electron

We describe here the preparation of poly(caprolactone) (PCL)-chitin nanofibrous mats by electrospinning from a blended solution of PCL and chitin dissolved in a cosolvent, 1,1,1,3,3,3-hexafluoro-2-propanol and trifluoroacetic acid. Scanning electron microscopy showed that the neutralized PCL-chitin nanofibrous mats were morphologically stable, with a mean diameter of340.5±2.6 nm, compared with a diameter of524.2±12.1 nm for PCL mats. The nanofibrous mats showed decreased water contact angles as the proportion of chitin increased. However, the tensile properties of nanofibrous mats containing30~50% (wt/wt) chitin were enhanced compared with PCL-only mats.In vitrostudies showed that the viability of human dermal fibroblasts (HDFs) for up to 7 days in culture was higher on composite (OD value:1.42±0.09) than on PCL-only (0.51±0.14) nanofibrous mats, with viability correlated with chitin concentration. Together, our results suggest that PCL-chitin nanofibrous mats can be used as an implantable substrate to modulate HDF viability in tissue engineering.

scholarly journals Surface Modification of Poly(lactic-co-glycolic acid) Microspheres with Enhanced Hydrophilicity and Dispersibility for Arterial Embolization

Materials ◽  
2019 ◽  
Vol 12 (12) ◽  
pp. 1959
Author(s):  
Jiao Wang ◽  
Jianbo Li ◽  
Jie Ren
Keyword(s):  
Glycolic Acid ◽  
Arterial Embolization ◽  
Contact Angles ◽  
In Vitro Cytotoxicity ◽  
Plga Microspheres ◽  
Sem Images ◽  
Water Contact ◽  
Prospective Application ◽  
Ft Ir

In this study, a series of poly(lactic-co-glycolic acid) (PLGA) microspheres with different particle sizes for arterial embolization surgery were prepared. The polydopamine (PDA) and polydopamine/polyethyleneimine (PDA/PEI) were respectively coated on the PLGA microspheres as shells, in order to improve the hydrophilicity and dispersibility of PLGA embolization microspheres. After modification, with the introduction of PDA and PEI, many hydrophilic hydroxyl and amine groups appeared on the surface of the PLGA@PDA and PLGA@PDA/PEI microspheres. SEM images showed the morphologies, sizes, and changes of the as-prepared microspheres. Meanwhile, the XPS and FT-IR spectra demonstrated the successful modification of the PDA and PEI. Water contact angles (WCAs) of the PLGA@PDA and PLGA@PDA/PEI microspheres became smaller, indicating a certain improvement in surface hydrophilicity. In addition, the results of in vitro cytotoxicity showed that modification had little effect on the biosafety of the microspheres. The modified PLGA microspheres suggest a promising prospective application in biomedical field, as the modified microspheres can reduce difficulties in embolization surgery.

Effects of Titanium Surface Wettability on Osteoblast Behavior In Vitro

2020 ◽  
Vol 985 ◽  
pp. 64-68
Author(s):  
Kenta Nisogi ◽  
Satoshi Okano ◽  
Sengo Kobayashi ◽  
Kensuke Kuroda ◽  
Takeaki Okamoto
Keyword(s):  
Contact Angle ◽  
Water Contact Angle ◽  
Titanium Surface ◽  
Pure Titanium ◽  
Hydrophobic Surface ◽  
Surface Wettability ◽  
In Vitro Assays ◽  
Water Contact ◽  
Heat Treated

Surface wettability is thought to influence the osteoconductivity of bone-substituting materials; however, the effects of surface wettability on osteoblast behavior are not well understood. In this study, we prepared both an as-polished pure titanium with a water contact angle (WCA) of 57° and heat-treated pure titanium with more hydrophobic surface and WCAs of 68°-98°. The effects of the surface wettability of pure titanium on osteoblast behaviors were evaluated by in vitro assays. Compared with the as-polished titanium, the proliferation rate of osteoblast increased on heat-treated titanium. This suggested that surface wettability affects osteoblast behaviors, meaning osteoconductivity is influenced by surface wettability.

scholarly journals A Study of Combining Elastin in the Chitosan Electrospinning to Increase the Mechanical Strength and Bioactivity

Marine Drugs ◽  
2021 ◽  
Vol 19 (3) ◽  
pp. 169
Author(s):  
Hengjie Su ◽  
Tomoko Fujiwara ◽  
Joel D. Bumgardner
Keyword(s):  
Bone Regeneration ◽  
Protein Extraction ◽  
Guided Bone Regeneration ◽  
Contact Angles ◽  
In Vivo Studies ◽  
Fiber Morphology ◽  
Water Contact ◽  
Degradation Rates ◽  
Chitosan Membranes

While electrospun chitosan membranes modified to retain nanofibrous morphology have shown promise for use in guided bone regeneration applications in in vitro and in vivo studies, their mechanical tear strengths are lower than commercial collagen membranes. Elastin, a natural component of the extracellular matrix, is a protein with extensive elastic property. This work examined the incorporation of elastin into electrospun chitosan membranes to improve their mechanical tear strengths and to further mimic the native extracellular composition for guided bone regeneration (GBR) applications. In this work, hydrolyzed elastin (ES12, Elastin Products Company, USA) was added to a chitosan spinning solution from 0 to 4 wt% of chitosan. The chitosan–elastin (CE) membranes were examined for fiber morphology using SEM, hydrophobicity using water contact angle measurements, the mechanical tear strength under simulated surgical tacking, and compositions using Fourier-transform infrared spectroscopy (FTIR) and post-spinning protein extraction. In vitro experiments were conducted to evaluate the degradation in a lysozyme solution based on the mass loss and growth of fibroblastic cells. Chitosan membranes with elastin showed significantly thicker fiber diameters, lower water contact angles, up to 33% faster degradation rates, and up to seven times higher mechanical strengths than the chitosan membrane. The FTIR spectra showed stronger amide peaks at 1535 cm−1 and 1655 cm−1 in membranes with higher concentrated elastin, indicating the incorporation of elastin into electrospun fibers. The bicinchoninic acid (BCA) assay demonstrated an increase in protein concentration in proportion to the amount of elastin added to the CE membranes. In addition, all the CE membranes showed in vitro biocompatibility with the fibroblasts.

scholarly journals Physical Properties and Biofunctionalities of Bioactive Root Canal Sealers In Vitro

Nanomaterials ◽  
2020 ◽  
Vol 10 (9) ◽  
pp. 1750
Author(s):  
Seung Bin Jo ◽  
Hyun Kyung Kim ◽  
Hae Nim Lee ◽  
Yu-Jin Kim ◽  
Kapil Dev Patel ◽  
...  
Keyword(s):  
Root Canal ◽  
Calcium Silicate ◽  
Biological Properties ◽  
Ion Release ◽  
Water Contact ◽  
Dimensional Changes ◽  
Sem Image ◽  
Potential Strategy ◽  
Root Canal Sealers

Calcium silicate-based bioactive glass has received significant attention for use in various biomedical applications due to its excellent bioactivity and biocompatibility. However, the bioactivity of calcium silicate nanoparticle-incorporated bioactive dental sealer is not much explored. Herein, three commercially available bioactive root canal sealers (Endoseal MTA (EDS), Well-Root ST (WST), and Nishika Canal Sealer BG (NBG)) were compared with a resin-based control sealer (AH Plus (AHP)) in terms of physical, chemical, and biological properties. EDS and NBG showed 200 to 400 nm and 100 to 200 nm nanoparticle incorporation in the SEM image, respectively, and WST and NBG showed mineral deposition in Hank’s balanced salt solution after 28 days. The flowability and film thickness of all products met the ISO 3107 standard. Water contact angle, linear dimensional changes, and calcium and silicate ion release were significantly different among groups. All bioactive root canal sealers released calcium ions, while NBG released ~10 times more silicon ions than the other bioactive root canal sealers. Under the cytocompatible extraction range, NBG showed prominent cytocompatibility, osteogenecity, and angiogenecity compared to other sealers in vitro. These results indicate that calcium silicate nanoparticle incorporation in dental sealers could be a potential strategy for dental periapical tissue regeneration.

Mechanobiological assessment of Ti-6Al-4V fabricated via selective laser melting technique: a review

2019 ◽  
Vol 25 (7) ◽  
pp. 1266-1284 ◽  
Author(s):  
Sahil Dhiman ◽  
Sarabjeet Singh Sidhu ◽  
Preetkanwal Singh Bains ◽  
Marjan Bahraminasab
Keyword(s):  
Selective Laser Melting ◽  
Human Life ◽  
Recent Decade ◽  
Biological Properties ◽  
Biological Behavior ◽  
Laser Melting ◽  
Content Type ◽  
Porous Ti

Purpose With technology advances, metallic implants claim to improve the quality and durability of human life. In the recent decade, Ti-6Al-4V biomaterial has been additively manufactured via selective laser melting (SLM) for orthopedic applications. This paper aims to provide state-of-the-art on mechanobiology of these fabricated components. Design/methodology/approach A literature review has been done to explore the potential of SLM fabricated Ti-6Al-4V porous lattice structures (LS) as bone substitutes. The emphasize was on the effect of process parameters and porosity on mechanical and biological properties. The papers published since 2007 were considered here. The keywords used to search were porous Ti-6Al-4V, additive manufacturing, metal three-dimensional printing, osseointegration, porous LS, SLM, in vitro and in vivo. Findings The properties of SLM porous biomaterials were compared with different human bones, and bulk SLM fabricated Ti-6Al-4V structures. The comparison was also made between LS with different unit cells to find out whether there is any particular design that can mimic the human bone functionality and enhance osseointegration. Originality/value The implant porosity plays a crucial role in mechanical and biological characteristics that relies on the optimum controlled process variables and design attributes. It was also indicated that although the mechanical strength (compressive and fatigue) of porous LS is not mostly close to natural cortical bone, elastic modulus can be adjusted to match that of cortical or cancellous bone. Porous Ti-6Al-4V provide favorable bone formation. However, the effect of design variables on biological behavior cannot be fully conclusive as few studies have been dedicated to this.

The Biological Behavior of Endothelial Progenitor Cells on Titanium Surface Immobilized by CD34 Antibody

2009 ◽  
Vol 79-82 ◽  
pp. 707-710 ◽  
Author(s):  
Cheng Chen ◽  
Jun Ying Chen ◽  
Quan Li Li ◽  
Jia Long Chen ◽  
Qiu Fen Tu ◽  
...  
Keyword(s):  
Progenitor Cells ◽  
Endothelial Progenitor Cells ◽  
Self Assembly ◽  
Titanium Surface ◽  
Protein A ◽  
Biological Behavior ◽  
Layer By Layer ◽  
Endothelial Progenitor ◽  
Implant Surface

The biological modification of biomaterials surface was an important means for surface endothelialization. In this work, an extracellular matrix-like (ECM-like) surface modification was developed for inducing endothelialization on titanium cardiovascular implant surface. To solve the problem of antibody denaturing caused in the randomly immobilizing, cluster of differentiation 34 (CD34) antibody was directly immobilized on titanium surface using a layer-by-layer self-assembly (LBL) technique. The biological behaviors of the endothelial progenitor cells (EPCs) on modified titanium surface were investigated by in vitro cell culture experiment. The results showed that the avidin, biotinylated protein A and the CD34 antibody were successfully assembled onto the NaOH etched titanium surface. The results of cells experiment suggested that the CD34 antibody immobilized surfaces promoted EPCs attachment and capture in vitro. It was believed that the response of adhesion, proliferation, differentiation of EPCs to titanium surface was regulated by modifying the surface chemistry which controlled the cell-biomaterial interactions. This work provided a surface biomodification means to increase the biocompatibility of titanium-based vascular implant surfaces.

scholarly journals Curcumin-Loaded Bacterial Cellulose/Alginate/Gelatin as A Multifunctional Biopolymer Composite Film

Molecules ◽  
2020 ◽  
Vol 25 (17) ◽  
pp. 3800 ◽  
Author(s):  
Nadda Chiaoprakobkij ◽  
Thapanar Suwanmajo ◽  
Neeracha Sanchavanakit ◽  
Muenduen Phisalaphong
Keyword(s):  
Oral Cancer ◽  
Bacterial Cellulose ◽  
Wound Care ◽  
Artificial Saliva ◽  
Water Vapor Permeability ◽  
Contact Angles ◽  
Vapor Permeability ◽  
Sem Images ◽  
Water Contact

Multifunctional biopolymer composites comprising mechanically-disintegrated bacterial cellulose, alginate, gelatin and curcumin plasticized with glycerol were successfully fabricated through a simple, facile, cost-effective mechanical blending and casting method. SEM images indicate a well-distributed structure of the composites. The water contact angles existed in the range of 50–70°. Measured water vapor permeability values were 300–800 g/m2/24 h, which were comparable with those of commercial dressing products. No release of curcumin from the films was observed during the immersion in PBS and artificial saliva, and the fluid uptakes were in the range of 100–700%. Films were stretchable and provided appropriate stiffness and enduring deformation. Hydrated films adhered firmly onto the skin. In vitro mucoadhesion time was found in the range of 0.5–6 h with porcine mucosa as model membrane under artificial saliva medium. The curcumin-loaded films had substantial antibacterial activity against E. coli and S. aureus. The films showed non-cytotoxicity to human keratinocytes and human gingival fibroblasts but exhibited potent anticancer activity in oral cancer cells. Therefore, these curcumin-loaded films showed their potential for use as leave-on skin applications. These versatile films can be further developed to achieve desirable characteristics for local topical patches for wound care, periodontitis and oral cancer treatment.

scholarly journals Fast Dissolving of Ferulic Acid via Electrospun Ternary Amorphous Composites Produced by a Coaxial Process

Pharmaceutics ◽  
2018 ◽  
Vol 10 (3) ◽  
pp. 115 ◽  
Author(s):  
Weidong Huang ◽  
Yaoyao Yang ◽  
Biwei Zhao ◽  
Gangqiang Liang ◽  
Shiwei Liu ◽  
...  
Keyword(s):  
Ferulic Acid ◽  
Drug Carriers ◽  
Contact Angles ◽  
Electrospinning Process ◽  
Poly Vinyl Alcohol ◽  
Coaxial Electrospinning ◽  
Water Contact ◽  
The Matrix ◽  
Combined Strategy

Enhancing the dissolution of insoluble active ingredients comprises one of the most important issues in the pharmaceutical and biomaterial fields. Here, a third generation solid dispersion (3rd SD) of ferulic acid was designed and fabricated by a modified coaxial electrospinning process. A traditional second generation SD (2nd SD) was also prepared by common one-fluid blending electrospinning and was used as a control. With poly(vinyl alcohol) as the fiber matrix and polyvinylpyrrolidone K10 as an additive in the 3rd SDs, the two electrospinning processes were investigated. The prepared 2nd and 3rd SDs were subjected to a series of characterizations, including X-ray diffraction (XRD), scanning electron microscope (SEM), hydrophilicity and in vitro drug dissolving experiments. The results demonstrate that both SDs were monolithic nanocomposites and that the drugs were amorphously distributed within the matrix. However, the 3rd SDs had better morphology with smaller size, narrower size distribution, and smaller water contact angles than the 2nd SDs. Dissolution tests verified that the 3rd SDs could release their loaded cargoes within 60 s, which was over three times faster than the 2nd SDs. Therefore, a combined strategy based on the modified coaxial electrospinning and the logical selections of drug carriers is demonstrated for creating advanced biomaterials.

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