scholarly journals Detection of Virulence Genes and Biofilm Forming Capacity of Diarrheagenic E. coli Isolated from Different Water Sources

Coatings ◽  
2021 ◽  
Vol 11 (12) ◽  
pp. 1544
Author(s):  
Sadaf Tariq ◽  
Sobia Tabassum ◽  
Sadia Aslam ◽  
Mika Sillanpaa ◽  
Wahidah H. Al-Qahtani ◽  
...  
Keyword(s):  
Drinking Water ◽  
Water Samples ◽  
Virulence Genes ◽  
Virulence Gene ◽  
Water Sources ◽  
Health Concern ◽  
Pcr Analysis ◽  
E Coli ◽  
Drinking Water Samples ◽  
Different Water Sources

Diarrheagenic Escherichia coli (DEC) are associated with frequent incidences of waterborne infections and pose health risk to individuals who contact water for domestic or recreational uses. Detection of DEC pathotypes in drinking water can be used as an indicator of fecal contamination. This study aimed to investigate the occurrence of DEC pathotypes and their capacity to form biofilms in drinking water samples collected from different water sources. In this study, PCR analysis was used to determine the occurrence of four clinically significant virulence genes of diarrheagenic E. coli, eaeA (Enteropathogenic E. coli), stx1, stx2 (Enterohemorrhagic E. coli) and sth (Enterotoxigenic E. coli), in drinking water samples (n = 35) by using specific primers and conditions. PCR amplicons were visualized by using agarose gel electrophoresis. A total of 12/35 (34%) samples were detected as positive for at least one of the four DEC virulence genes and 11/12 (91%) E. coli isolates harbored virulence gene while 1/12 (8%) E. coli isolates harbored none. The eaeA and sth genes were the most detected genes (75%), while stx1 and stx2 genes were least detected genes (66%). Biofilm assay confirmed that ETEC pathotypes can cause damage in enteric walls by attaching and effacing to persist diarrheal conditions. This study indicated that drinking water of different sources is contaminated with potential DEC pathotypes and it can be a source of diarrheal diseases. The amplification of four virulence genes associated with DEC pathotypes (EPEC, EHEC and ETEC) in drinking water demonstrates that potentially virulent DEC pathotypes are distributed in water sources and may be a cause of health concern. There is, therefore, an urgent need to monitor DEC pathotypes in drinking water.

scholarly journals Evaluation of Multi-Drug Resistant E. coli from Drinking Water Sources in a Tertiary Institution

2020 ◽  
pp. 1-9
Author(s):  
Udoh I.P., Iloghalu ◽  
Iloghalu, Ijenwa Amarachi ◽  
Aladenika S.T.
Keyword(s):  
Drinking Water ◽  
Multidrug Resistance ◽  
Water Samples ◽  
Antimicrobial Agents ◽  
Water Sources ◽  
Bottled Water ◽  
Health Concern ◽  
Storage Tanks ◽  
E Coli ◽  
Drinking Water Sources

Infectious diseases control of recent is a major health concern globally due to high increase in number of microorganisms that are resistant to conventional antimicrobial agents. This study aimed at ascertaining the microbiological quality and multiple antibiotic resistance profile of E. coli strains isolated from different sources of drinking water. A total of 136 water samples from different drinking water sources, including the storage tanks (the school and the commercial storage tank), sachet and bottle water were obtained from University of Nigeria Enugu Campus and analyzed. Standard microbiological techniques were employed for bacteria isolation, identification and antibiogram. From the water samples collected 25 E. coli strains were isolated. The school storage tanks account for 60% of the isolates, while bottled water showed no growth. 92% of the E. coli isolated showed resistance to the tested antibiotics. Resistant were higher with Augmentin (64%), Chloramphenicol (48%) and Streptomycin 11 (44%) while most were sensitive to Tarivid and Perfloxacine (100%). Isolates from school storage water sources showed the highest resistance to Augmentin (76.5%) while those from commercial storage water sources showed the highest resistance to Streptomycin (66.7%). Out of the 23 (92%) antibiotics resistant E. coli isolates 18 (78.3%) were multidrug resistance (MDR). The school storage water sources had the highest number of MDR E. coli 14 (77.8%) followed by the commercial storage water sources 4 (22.2%), but the sachet and bottled water had no MDR E. coli. In conclusion, drinking water may potentially contribute to the source of multidrug resistance E. coli in this community

Virulence and antimicrobial resistance genes associated with the in-vivo pathogenicity of avian pathogenic E. coli isolates

2021 ◽  
Vol 1 (1) ◽  
pp. 17-20
Author(s):  
Ahmed Abd El-Mawgoud ◽  
Azza El-Sawah ◽  
Soad Nasef ◽  
Al-Hussien Dahshan ◽  
Ahmed Ali
Keyword(s):  
Antimicrobial Resistance ◽  
Resistance Genes ◽  
Broiler Chickens ◽  
Virulence Genes ◽  
Virulence Gene ◽  
Health Concern ◽  
Pathogenicity Test ◽  
E Coli ◽  
Antimicrobial Resistance Genes

In the current study, ten avian pathogenic E. coli (APEC) isolates of the most predominant APEC serogroups isolated from broiler chickens in Egypt were screened for their virulence and antimicrobial resistance genes pattern using PCR. Five selected virulence gene patterns were further investigated for their in-vivo pathogenicity test. Results showed a 100% prevalence of the β-lactams and tetracyclines resistance genes. However, aminoglycoside and quinolone resistance genes were not detected. Also, 80% of the tested isolates harbored mcr-1 gene, colistin resistance gene. In-vivo pathogenic strains consistently harbored the virulence gene pattern of fimH, fimA, papC, iutA, and tsh. Additionally, the tsh gene was consistently detected with lethal APEC isolates in day-old chicks. These results highlighted the high prevalence of antimicrobial and virulence genes in APEC that potentially represent a public health concern. In this study, the virulence genes fimH, fimA, papC, iutA, and tsh were the most common virulence gene patterns associated with pathogenicity in day-old chicks.

scholarly journals Borehole water: a potential health risk to rural communities in South Africa

2018 ◽  
Vol 19 (1) ◽  
pp. 128-136 ◽  
Author(s):  
S. Taonameso ◽  
L. S. Mudau ◽  
A. N. Traoré ◽  
N. Potgieter
Keyword(s):  
Drinking Water ◽  
Rural Communities ◽  
Water Samples ◽  
Rural Areas ◽  
Total Coliform ◽  
Water Sources ◽  
Potential Health ◽  
Point Mapping ◽  
E Coli ◽  
Mapping Tool

Abstract Sporadic outbreaks of diarrhoea in children in the Vhembe rural areas could be an indication of contamination in drinking water sources. In areas where improved water sources are used, not all rural households experience the benefits of these improved water sources. Water samples were collected from boreholes in three wards in the Vhembe District to determine microbiological risks over a 5-month period. A Water Point Mapping tool was used to indicate the borehole distribution. Water samples were taken from each functional borehole and analysed for total coliform and Escherichia coli counts, electrical conductivity, pH and temperature. A multiplex PCR protocol was used for identification of pathogenic E. coli. A total of 125 boreholes were identified of which only 12 were functional. Seven boreholes tested positive for total coliforms and E. coli counts. Four boreholes (33.3%) tested positive for diarrhoeagenic E. coli. Fifty-eight percent (58%) of water samples were without health risks, 17% were low risk and 25% could cause infection according to the South African water quality standards. This study indicated the importance of the role of the Municipalities and the maintenance plans that need to ensure that all boreholes are functional and provide safe drinking water to the rural communities.

scholarly journals Investigation of Drinking Water Quality in Kosovo

2013 ◽  
Vol 2013 ◽  
pp. 1-9 ◽  
Author(s):  
Fatlume Berisha ◽  
Walter Goessler
Keyword(s):  
Drinking Water ◽  
Water Samples ◽  
Inductively Coupled Plasma ◽  
Tap Water ◽  
Health Concern ◽  
Inductively Coupled ◽  
Minor Elements ◽  
Important Health ◽  
Different Sources ◽  
Drinking Water Samples

In the recent years, not much environmental monitoring has been conducted in the territory of Kosovo. This study represents the first comprehensive monitoring of the drinking water situation throughout most of the territory of Kosovo. We present the distribution of major and minor trace elements in drinking water samples from Kosovo. During our study we collected 951 samples from four different sources: private-bored wells; naturally flowing artesian water; pumped-drilled wells; and public water sources (tap water). The randomly selected drinking water samples were investigated by routine water analyses using inductively coupled plasma mass spectrometry (ICPMS) for 32 elements (Li, Be, B, Na, Mg, Al, K, Ca, V, Cr, Mn, Fe, Co, Ni, Cu, Zn, Ga, As, Rb, Sr, Mo, Ag, Cd, Sn, Sb, Te, Ba, Tl, Pb, Bi, Th, U). Even though there are set guidelines for elemental exposure in drinking water worldwide, in developing countries, such as Kosovo, the lack of monitoring drinking water continues to be an important health concern. This study reports the concentrations of major and minor elements in the drinking water in Kosovo. Additionally, we show the variation of the metal concentration within different sources. Of the 15 regulated elements, the following five elements: Mn, Fe, Al, Ni, As, and U were the elements which most often exceeded the guidelines set by the EU and/or WHO.

scholarly journals Analysis of heavy metals and coliform in samples of drinking water collected from municipal ward offices of western suburbs of Mumbai, India

2014 ◽  
Vol 15 (1&2) ◽  
pp. 99-107
Author(s):  
G.V Zodape
Keyword(s):  
Heavy Metals ◽  
Drinking Water ◽  
Water Samples ◽  
Fecal Contamination ◽  
E Coli ◽  
Metallic Sheen ◽  
Differential Medium ◽  
Drinking Water Samples

14 drinking water samples were collected from Vile Parle to Dadar of Suburban’s of (P-South ward offices of B.M.C (Bombay Municipal Corporation) Mumbai in the month from June to December 2012. The samples of drinking-water were analyzed for Cu, Zn, Mn, Fe, As, Cr, Ni, Pb, Cd and Hg. From the results so obtained, the contamination due to heavy metals – Zn (3.115 ppm and 7.816 ppm), Mn (3.115 ppm and 7.426 ppm), Fe (1.124 ppm and 2.872 ppm), As (0.011 ppm to 0.091 ppm), Cr (0.188 ppm and 0.998 ppm ), Pb (1.587 ppm and 4.56 ppm) and Cd (0.011 ppm and 0.051 ppm ) was found to be high whereas the contamination due to Cu (0.012 ppm and 0.313 ppm), Ni (0.126 ppm and 0.774 ppm), were found below the acceptable limits and no Hg was detected in the samples of drinking-water. In the present work, MacConkey Broth was used as a differential medium for detection and enumeration of coliforms from a wide variety water samples. The presence of positive doubtful presumptive test immediately suggests that the water is non potable (i.e., both acid and gas develops in a tube after 48 hours incubation). Confirmation suggests that there is fecal contamination in the water under investigation and hence it is non potable. All the fourteen samples (streaked from positive Ma cConkey broth tubes) were found to be contaminated with E. coli, which was further confirmed by the presence of colonies with green metallic sheen observed under a microscope confirmed that the said samples were contaminated with E. coli - the major indicator of fecal contamination.

scholarly journals Sanitary Status and Occurrence of Some Water-Borne Pathogens in Well and Surface Waters of Panhauya Community and Ahmadu Bello University Farm, Zaria

2020 ◽  
Vol 5 (2) ◽  
pp. 138-147
Author(s):  
S. L. Afegbua ◽  
Keyword(s):  
Drinking Water ◽  
Surface Waters ◽  
Water Samples ◽  
Universal Access ◽  
Microbiological Quality ◽  
Health Concern ◽  
Salmonella Spp ◽  
Open Defecation ◽  
E Coli ◽  
Water Borne

The Sustainable Development Goal on sanitation aims to achieve universal access to good health, affordable drinking water, sanitation and an end to open defeacation by 2030. The recent ranking of Nigeria as first globally for open defecation is of public and environmental health concern. This study assessed the sanitary condition and the microbiological quality of well and surface waters of Panhauya community and Ahmadu Bello University farm, Zaria, and the antibiogram of the bacterial isolates.. Based on the WHO criteria, the sanitary inspection showed that 16.7%, 54.2%, 25% and 4.2% of the water sampling points had a very high, high, intermediate and low risk of contamination respectively. Occurrence of Escherichia coli, Giardia lamblia, Entamoeba histolytica, Pseudomonas aeruginosa, Salmonella spp and Vibrio cholerae in water samples from Panhuaya community was 87.5%, 75%, 68.8%, 50%, 25% and 12.5% respectively. In ABU farm Shika, the occurrence was; E. coli (75%), E. histolytica (63%), G. lamblia and Salmonella spp. All E. coli isolates exhibited high multidrug resistance to antibiotics screened with a MAR index of 0.3-0.8. The drinking water sources in Panhuaya and ABU farm were unsafe and the presence of these pathogens in the water samples may be attributed to a number of factors including poor sanitation, manure application and open defecation practice. This indicates a public health risk to the residents and emphasises the need for safe water supplies sanitation and antibiotic stewardship. Keywords: Well water; surface water; sanitary inspection; open defecation; water-borne pathogens; Zaria.

Characterisation of multidrug resistant Escherichia coli isolated from two commercial lettuce and spinach supply chains

2021 ◽  
Author(s):  
Muneiwa T. Ratshilingano ◽  
Erika Margarete du Plessis ◽  
Stacey Duvenage ◽  
Lise Korsten
Keyword(s):  
Escherichia Coli ◽  
Antibiotic Resistance ◽  
Irrigation Water ◽  
Virulence Genes ◽  
Disease Outbreaks ◽  
Virulence Gene ◽  
Multidrug Resistant ◽  
Water Sources ◽  
Point Of Sale ◽  
E Coli

ABSTRACT Leafy green vegetables have increasingly been reported as a reservoir of multidrug-resistant pathogenic Enterobacteriaceae; with Shiga toxin- producing Escherichia coli frequently implicated in disease outbreaks worldwide.  This study aimed to determine the presence and characteristics of antibiotic resistance, diarrheagenic virulence genes and phylogenetic groupings of E. coli isolates (n=51) from commercially produced lettuce and spinach from the farm, through processing and at the point of sale.  Multidrug resistance was observed in 33 of the 51 E. coli isolates (64.7%); with 35.7% (n=10/28) being generic and 100% (n=23/23) Extended Spectrum β-lactamase/AmpC- producing.  Resistance of E. coli isolates was observed against neomycin (100%; n=51/51), ampicillin (70.6%; n=36/51), amoxycilin (68.6%; n=35/51), tetracycline (45%; n=23/51), trimethoprim/ sulfamethoxazole (43%; n=22/51), chloramphenicol (25.5%; n=13/51), augmentin (11.8%; n=6/51) and gentamicin (7.8%; n=4/51); with 100% (n=51/51) susceptibility to imipenem. Virulence gene eae was detected in two E. coli isolates from irrigation water sources only, while none of the other virulence genes tested for were detected.   Most of the E. coli strains belonged to phylogenetic group B2 (25.5%; n=13), B1 (19.6%; n=10) and A (17.6%; n=9); with D (5.9%; n=3) less distributed. Although diarrheagenic E. coli were not detected, antibiotic resistance in E. coli prevalent in the supply chain was evident. Additionally, a clear link between E. coli isolates from irrigation water sources and leafy green vegetables through DNA fingerprinting was established which indicates the potential transfer of E. coli from irrigation water to minimally processed leafy green vegetables.

scholarly journals Detection of virulence genes of diarrheagenic Escherichia coli strains from drinking water in Khartoum State

2020 ◽  
Author(s):  
Ehssan H. Moglad ◽  
Omima Abdl El Jalil Adam ◽  
Maram M. Alnosh ◽  
Hisham N. Altayb
Keyword(s):  
Escherichia Coli ◽  
Drinking Water ◽  
Genomic Dna ◽  
Multiplex Polymerase Chain Reaction ◽  
Virulence Genes ◽  
Chain Reaction ◽  
E Coli ◽  
Diarrheagenic Escherichia Coli ◽  
Polymerase Chain ◽  
Drinking Water Samples

Abstract This study aimed to determine the prevalence of virulence genes in all the diarrheagenic Escherichia coli DEC strains (EAEC, EHEC, EIEC, EPEC, and ETEC) isolated from drinking water from Khartoum State, Sudan. A total of 46 drinking water samples obtained from different water sources were analyzed for the presence of E. coli as fecal contamination indicator and the antimicrobial-resistant pattern of isolated E. coli DEC strain was investigated. The bacterial genomic DNA was used as a template for multiplex polymerase chain reaction (MPCR) for the detection of the EHEC (stx gene), EIEC (ipaH gene), EPEC (eae gene), and EAEC (aggR gene) as virulence and biomarker genes. Our results showed that ipaH gene was found in 41.3% (19/46) of isolates, and aggR gene detected in 30.4% (14/46) of isolates. Both aggR and ipaH were found positive in 9 (19.5%) isolates and as well the combination of aggR and stx genes were detected in 2 (4.3%) isolates. In conclusion, this report confirmed the presence of DEC strains in drinking water from different resources and locations. Such findings require separate future clinical research studies to examine waterborne pathogens that exist in this state's water and find a management solution to stop or avoid potential outbreaks.

scholarly journals Abundance of pathogenic Escherichia coli, Salmonella typhimurium and Vibrio cholerae in Nkonkobe drinking water sources

2006 ◽  
Vol 4 (3) ◽  
pp. 289-296 ◽  
Author(s):  
Maggy N. B. Momba ◽  
Veronica K. Malakate ◽  
Jacques Theron
Keyword(s):  
Escherichia Coli ◽  
Drinking Water ◽  
Salmonella Typhimurium ◽  
Vibrio Cholerae ◽  
Water Samples ◽  
Pathogenic Bacteria ◽  
Culture Media ◽  
Water Sources ◽  
E Coli ◽  
Drinking Water Sources

In order to study the prevalence of enteric pathogens capable of causing infection and disease in the rural communities of Nkonkobe, bacterial isolates were collected from several surface water and groundwater sources used by the community for their daily water needs. By making use of selective culture media and the 20E API kit, presumptive Escherichia coli, Salmonella spp. and Vibrio cholerae isolates were obtained and then analysed by polymerase chain reaction assays (PCR). The PCR successfully amplified from water samples a fragment of E. coli uidA gene that codes for β-D-glucuronidase which is a highly specific characteristic of enteropathogenic E. coli, enterotoxigenic E. coli and entero-invasive E. coli. The PCR also amplified the epsM gene from water samples containing toxigenic V. cholerae. Although E. coli was mostly detected in groundwater sources, toxigenic V. cholerae was detected in both surface and groundwater sources. There was a possibility of Salmonella typhimurium in Ngqele and Dyamala borehole water samples. The presence of these pathogenic bacteria in the above drinking water sources may pose a serious health risk to consumers.

scholarly journals Prevalence and Virulence Gene Profiles of Escherichia coli O157 from Cattle Slaughtered in Buea, Cameroon

2020 ◽  
Author(s):  
Elvis Achondou Akomoneh ◽  
Seraphine Nkie Esemu ◽  
Achah Jerome Kfusi ◽  
Roland N. Ndip ◽  
Lucy M. Ndip
Keyword(s):  
Escherichia Coli ◽  
Virulence Genes ◽  
Foodborne Pathogen ◽  
Virulence Gene ◽  
Latex Agglutination ◽  
Health Concern ◽  
E Coli ◽  
Uremic Syndrome ◽  
Human Infections ◽  
Pcr Targeting

ABSTRACTBackgroundEscherichia coli O157 is an emerging foodborne pathogen of great public health concern. It has been associated with bloody diarrhoea, haemorrhagic colitis and haemolytic uremic syndrome in humans. Most human infections have been traced to cattle and the consumption of contaminated cattle products. In order to understand the risk associated with the consumption of cattle products, this study sought to investigate the prevalence and identify virulence genes in E. coli O157 from cattle in Cameroon.MethodA total of 512 rectal samples were obtained and analysed using conventional bacteriological methods (enrichment on modified Tryptone Soy Broth and selective plating on Cefixime-Tellurite Sorbitol Mac-Conkey Agar) for the isolation of E. coli O157. Presumptive E. coli O157 isolates were confirmed serologically using E. COLIPRO™ O157 latex agglutination test and molecularly using PCR targeting the rfb gene in the isolates. Characterisation of the confirmed E. coli O157 strains was done by amplification of stx1, stx2, eaeA and hlyA virulence genes using both singleplex and multiplex PCR.ResultsE. coli O157 was detected in 56 (10.9%) of the 512 samples examined. The presence of the virulence genes stx2, eaeA and hylA was demonstrated in 96.4% (54/56) of the isolates and stx1 in 40 (71.4%) of the 54. The isolates exhibited three genetic profiles (I-III) with I (stx1, stx2, eaeA and hlyA) being the most prevalent (40/56; 71.4%) while two isolates had none of the virulence genes tested.ConclusionA proportion of cattle slaughtered in abattoirs in Buea are infected with pathogenic E. coli O157 and could be a potential source of human infections. We recommend proper animal food processing measures and proper hygiene be prescribed and implemented to reduce the risk of beef contamination.

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