scholarly journals Biosynthesis of CeO2 Nanoparticles Using Egg White and Their Antibacterial and Antibiofilm Properties on Clinical Isolates

Crystals ◽  
2021 ◽  
Vol 11 (6) ◽  
pp. 584
Author(s):  
Shalendra Kumar ◽  
Faheem Ahmed ◽  
Nagih M. Shaalan ◽  
Osama Saber
Keyword(s):  
Bacterial Infections ◽  
Antibacterial Properties ◽  
Spherical Shape ◽  
Inhibition Zone ◽  
Egg White ◽  
Ceo2 Nanoparticles ◽  
Inorganic Materials ◽  
Ambient Conditions ◽  
Uniform Size ◽  
Tem Analysis

Bio-inspired synthesis is a novel and attractive environmentally friendly route to generating inorganic materials. In this work, the preparation of CeO2 NPs using egg white and investigation of their antibacterial properties both in liquid and solid growth medium against Escherichia coli and Staphylococcus aureus bacteria were reported. The CeO2 nanoparticles were characterized using X-ray diffraction (XRD), Field emission transmission electron microscope (FETEM), UV-Vis, Raman, and antibacterial measurements. The results from XRD and TEM analysis showed that the prepared nanoparticles were a single phase in the nano regime (5–7 nm) with spherical shape and uniform size distribution. Optical properties reflected the characteristics peaks of CeO2 in the UV-Vis range with a bandgap ~2.80 eV. The antibacterial activity of the synthesized NPs was achieved under ambient conditions with different bacteria and the results showed that the properties were different for both the bacteria. The highest activity with an inhibition zone of about 22 mm against S. aureus was obtained as compared with the 19 mm zone of inhibition obtained with E.coli. This finding will be of major significance that indicates a possibility to develop CeO2 NPs as antibacterial agents against extensive microorganisms to control and prevent the spread and persistence of bacterial infections.

scholarly journals Structural, Optical, Electrical and Antibacterial Properties of Fe-Doped CeO2 Nanoparticles

Crystals ◽  
2021 ◽  
Vol 11 (12) ◽  
pp. 1594
Author(s):  
Shalendra Kumar ◽  
Suliman Yousef AlOmar ◽  
Kavita Kumari ◽  
Fadwa Albalawi ◽  
Rajesh Kumar ◽  
...  
Keyword(s):  
Dielectric Constant ◽  
Ac Conductivity ◽  
Bacterial Infections ◽  
Antibacterial Properties ◽  
Ceo2 Nanoparticles ◽  
Fe Doping ◽  
Ambient Conditions ◽  
Xrd Pattern ◽  
Vis Spectroscopy ◽  
Doped Ceo2

This paper reports the structural, optical and antimicrobial study of Ce1−xFexO2−δ (0≤ x ≤20) nanoparticles (NPs) synthesized using a microwave-assisted hydrothermal method. The XRD pattern analysed using Rietveld refinement method clearly infers that all the samples exhibit single phase nature and exclude the possibility of an impurity phase. The lattice parameters and unit cell volume were found to decrease with an increase in Fe-doping content in CeO2 nanoparticles. The crystalline size determined using XRD pattern and TEM micrographs was found to decrease with Fe doping in CeO2. Selective area electron diffraction (SAED) pattern also demonstrated the crystalline nature of the Fe-CeO2 nanoparticles. Optical properties studied using UV–vis spectroscopy indicated that band gap decreased with an increase in Fe doping. The electrical properties have been investigated via dielectric constant, dielectric loss and AC conductivity. The dielectric constant was found to increase in the Fe-doped CeO2 nanoparticles, while AC conductivity was found to be reduced, which shows good dielectric behaviour of the Fe-doped CeO2 nanoparticles. The antibacterial activity of the synthesized NPs was achieved under ambient conditions with different bacteria, and the results showed that the properties were different for both bacteria. The antimicrobial activity reflects the possibility to develop Fe-doped CeO2 NPs as antibacterial agents against extensive microorganisms to control and prevent the spread and persistence of bacterial infections.

scholarly journals Microwave Mediated Fast Synthesis of Silver Nanoparticles and Investigation of Their Antibacterial Activities for Gram-Positive and Gram-Negative Microorganisms

Crystals ◽  
2021 ◽  
Vol 11 (6) ◽  
pp. 666
Author(s):  
Faheem Ahmed ◽  
Suliman Yousef AlOmar ◽  
Fadwa Albalawi ◽  
Nishat Arshi ◽  
Sourabh Dwivedi ◽  
...  
Keyword(s):  
Silver Nanoparticles ◽  
Bacterial Infections ◽  
Antibacterial Properties ◽  
Spherical Shape ◽  
Ammonium Bromide ◽  
Face Centered Cubic ◽  
Gram Positive ◽  
Tem Analysis ◽  
Gram Negative ◽  
Vis Spectroscopy

Herein, a simple one-step microwave irradiation technique has been used to synthesize the silver nanoparticles using silver nitrate (AgNO3) solution and cetyltrimethyl ammonium bromide (CTAB) as a stabilizing agent. The crystals of as-prepared nanoparticles were studied using X-ray diffraction (XRD) and a selected area electron diffraction (SAED) pattern, confirming the single-phase face-centered cubic structure. The optical property measured using UV-Vis spectroscopy shows an absorption maximum at 420 nm, which also confirms the formation of silver nanoparticles. Transmission electron microscopy (TEM) analysis revealed that the silver nanoparticles have a spherical shape with an average diameter of ~6 nm. The antibacterial properties of silver nanoparticles were investigated using both Gram-positive and Gram-negative microorganisms, such as Staphylococcus aureus, Pseudomonas aeruginosa, andEscherichia coli. Klebsiella pneumoniae, and Candida albicans. Results showed a highest zone of inhibition of about 35 mm against P. aeruginosa as compared with E. coli (21 mm), S. aureus (30 mm), K. pneumonia (28 mm), and C. albicans (29 mm). These studies suggested that silver nanoparticles prepared by this fast and effective method might be developed as antibacterial agents against an extensive range of microorganisms to control and stop the spreading and persistence of bacterial infections.

scholarly journals Synthesis of Chitosan/Zinc Oxide Nanoparticles Stabilized by Chitosan via Microwave Heating

2019 ◽  
Vol 14 (2) ◽  
pp. 450 ◽  
Author(s):  
Nurul Amira Ahmad Yusof ◽  
Norashikin Mat Zain ◽  
Norlin Pauzi
Keyword(s):  
Zinc Oxide ◽  
Microwave Heating ◽  
Zno Nanoparticles ◽  
Bacterial Infections ◽  
Pathogenic Bacteria ◽  
Antibacterial Properties ◽  
Inhibition Zone ◽  
Mean Value ◽  
Average Crystalline Size ◽  
Xrd Patterns

Nowadays, zinc oxide (ZnO) has attracted attention in research and development because of its remarkable antibacterial properties. Chitosan/ZnO nanoparticles were successfully synthesized via microwave heating. The objectives of this work were to investigate the effect of stabilizer, power heating and time heating on size of chitosan/ZnO nanoparticles and to determine antibacterial activity against pathogenic bacteria, where chitosan was used as a stabilizing agent. Chitosan/ZnO nanoparticles were analyzed  by Fourier Transform Infra Red (FTIR), X-ray Diffraction (XRD), Field Emission Scanning Electron Microscopy (FESEM), and Zetasizer instrument. The power heating and time heating were varied from 400 to 800 Watt and 4 to 8 minutes, respectively. The presence of chitosan has role on preventing the nanoparticles from agglomeration by producing a milky solution of chitosan/ZnO nanoparticles without any suspensions. The increase of power  and time heating improved the size of nanoparticles. The peak in FTIR spectrum at around 427 cm-1 was confirmed the existence of the ZnO phase. XRD patterns showed that the chitosan/ZnO nanoparticles materials were pure phase with average crystalline size is 130 nm. FESEM revealed that chitosan/ZnO nanoparticles were uniformly distributed with the mean value of size is 70 nm and spherical shaped. Further impact of power and time heating on the size of the chitosan/ZnO nanoparticles can be shown by a nanoparticles size distribution with the average of 30 to 90 nm. The results showed that chitosan/ZnO nanoparticles have displayed an antibacterial inhibition zone against Gram-positive S. aureus and Gram-negative E. coli which 16.0 and 13.3 mm, respectively. Chitosan/ZnO nanoparticles were synthesized in this work presented have potential application to prevent bacterial infections. Copyright © 2019 BCREC Group. All rights reserved 

scholarly journals Antibacterial properties of an Indian traditional medicinal plant, Rhynchosia scarabaeoides (L.) DC. (Fabaceae)

2011 ◽  
Vol 1 (1) ◽  
pp. 17 ◽  
Author(s):  
Surekha Challa ◽  
Kiran K. Rajam ◽  
Vishnu V. V. Satyanarayana Kasapu ◽  
Suresh Kumar Tanneeru ◽  
Venkata Siva Satyanarayana Kantamreddi
Keyword(s):  
Ethyl Acetate ◽  
Bacterial Infections ◽  
Antibacterial Properties ◽  
Inhibition Zone ◽  
Antibacterial Activities ◽  
Traditional Healers ◽  
Diffusion Method ◽  
Bacterial Strains ◽  
Plant Parts ◽  
Ethanol Extracts

<em>Rhynchosia scarabaeoides </em>(L.) DC plant parts are extensively used by traditional healers in India to treat a variety of bacterial diseases, such as dysentery, diarrohea and skin disorders. This article reports the antibacterial activities of n-hexane, ethyl acetate and ethanol extracts belonging to the leaf, stem and root parts of <em>R. scarabaeoides</em> against five bacterial strains, <em>Bacillus subtilis</em>, <em>Escherichia coli</em>, <em>Klebseilla pneumonia</em>, <em>Proteus vulgaris</em> and <em>Staphylococcus aureus</em>, using an agar gel diffusion method. The range of inhibition zone (IZ) was found to be 15-24 mm and the minimum inhibitory activity (MIC) was found to be 1 mL/well. The IZ was found to be higher in ethyl acetate extracts while this was moderate in ethanol extracts, and no activity was seen with n-hexane extracts or root extracts. The MIC value of leaf ethyl acetate extract was found to be 1 mg against bacterial strains <em>P. vulgaris</em> and <em>S. aureus</em>, whereas 2 mg was found against <em>B. subtilis</em>, <em>K. pneumoniae</em> and <em>E. coli</em>. These results support the traditional usage of R. scarabaeoides plant parts in the treatment of bacterial infections. Interestingly, this plant was screened for antibacterial activity for the first time and was found to be active. Detailed chemical investigations are, therefore, warranted.

scholarly journals Photosynthesis of Carboxymethyl Starch-Stabilized Silver Nanoparticles and Utilization to Impart Antibacterial Finishing for Wool and Acrylic Fabrics

2013 ◽  
Vol 2013 ◽  
pp. 1-9 ◽  
Author(s):  
M. A. El-Sheikh ◽  
L. K. El Gabry ◽  
H. M. Ibrahim
Keyword(s):  
Silver Nanoparticles ◽  
Bacterial Growth ◽  
Irradiation Time ◽  
Synthesis Method ◽  
Antibacterial Properties ◽  
Spherical Shape ◽  
Inhibition Zone ◽  
Water Soluble ◽  
Carboxymethyl Starch ◽  
Antibacterial Performance

The water soluble photoinitiator (PI) 4-(trimethyl ammonium methyl) benzophenone chloride/UV system is used in the synthesis of silver nanoparticles (AgNPs). Green synthesis method involved using PI/UV system, carboxymethyl starch (CMS), silver nitrate, and water. AgNPs obtained had a spherical shape morphology and a size of 1–7 nm. To impart antibacterial properties, wool and acrylic fabrics were treated with AgNPs obtained. The PI/UV system was further utilized to fix AgNPs onto wool and acrylic fabrics by photocrosslinking to impart durable antibacterial properties. The effect of irradiation time on the antibacterial performance before and after repeated washing cycles was studied. S. aureus (as G +ve) and E. coli (as G −ve) were used to estimate the antibacterial performance of the finished fabrics. The antibacterial performance was directly proportional to the irradiation time but inversely proportional to the number of washing cycles. However, after the 15th washing cycle, samples still have bacteriostatic effect; that is, although they show zero inhibition zone, they cannot be attacked by the bacterial growth and do not inhibit the bacterial growth. AgNPs finished wool fabrics showed more antibacterial activity than those of AgNPs finished acrylic fabrics.

scholarly journals Aktivitas Antibakteri Ekstrak Daun Widuri Terhadap Escherichia Coli Dan Staphylococcus Aureus

2020 ◽  
Vol 2 (1) ◽  
pp. 1-9
Author(s):  
Siti Zamilatul Azkiyah
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Bacterial Infections ◽  
Leaf Extract ◽  
Antibacterial Properties ◽  
Ethanol Extract ◽  
Inhibition Zone ◽  
The Body ◽  
And Staphylococcus Aureus

Antibiotics have a very important role in fighting bacterial infections in the body. However, the results given so far have not been optimal in overcoming the problem of bacterial infection. An alternative solution is to develop and study plants that have medicinal potential, for example thistle leaves (Calotrophis gigantean). The purpose of this study was to determine the antibacterial activity of thistle leaf extract against Escherichia coli and Staphylococcus aureus. This research is a laboratory-scale experimental research that is to test the activity of thistle leaf extract against Escherichia coli and Staphylococcus aureus including sample identification, extraction, activity testing by observing the inhibition zone of the two tested bacteria. Based on the results of the study stated that the ethanol extract of 70% Widuri leaves (Calotrophis gigantea) had antibacterial activity and could inhibit the growth of Escherichia coli and Staphylococcus aureus bacteria. The active compounds that are thought to have antibacterial properties in the ethanol extract of Widuri leaves (Calotrophis gigantea) are flavonoids, alkaloids and terpenoids.   Keywords: Widuri leaves, Escherichia coli, Staphylococcus aureus, Antibacterial ABSTRAK   Pentingnya penanggulangan infeksi bakteri menggunakan antibiotik adalah hal yang lazim dalam terapi farmakologi. Dampak dari pemberian antibiotik masih belum maksimal dalam penanggulangannya terhadap infeksi bakteri. Pemilihan alternatif bahan alam dengan kandungan kimia yang memiliki potensi sebagai antibiotik dapat menjadi pilihan lain dalam mengurangi penggunaan antibiotik sintetik, misalnya tanaman widuri (Calotrophis gigantean). Tujuan dari penelitian ini yaitu untuk mengetahui aktivitas antibakteri ekstrak daun widuri terhadap Escherichia coli dan Staphylococcus aureus. Desain penelitian meliputi identifikasi sampel, ekstraksi, hingga uji aktivitas dengan mengamati zona hambat dari kedua bakteri uji. Hasil penelitian menunjukkan bahwa ekstrak etanol 70% daun Widuri memiliki aktivitas antibakteri dan dapat menghambat pertumbuhan bakteri Escherichia coli dan Staphylococcus aureus. Senyawa aktif yang diduga bersifat antibakteri pada ekstrak etanol daun Widuri adalah senyawa golongan flavonoid, alkaloid dan terpenoid. Kata Kunci: Daun Widuri, Escherichia coli, Staphylococcus aureus, Antibakteri

Antibacterial Activity of Ag-TiO2 Nanoparticles with Various Silver Contents

2013 ◽  
Vol 756 ◽  
pp. 238-245 ◽  
Author(s):  
Nur Hidayati Ahmad Barudin ◽  
Srimala Sreekantan ◽  
Ong Ming Thong ◽  
Geethaa Sahgal
Keyword(s):  
Antibacterial Activity ◽  
Metallic Nanoparticles ◽  
Sol Gel ◽  
Antibacterial Properties ◽  
Antimicrobial Properties ◽  
Bacterial Count ◽  
Inhibition Zone ◽  
Light Irradiation ◽  
Great Promise ◽  
Uniform Size

Bacterial are highly transmitted in our environment and have been identified as a primary contributor to the problem of indoor air quality and consequently lead to the illness of the occupants. Recently, nanotechnology represents an innovative approach to develop new formulations based on metallic nanoparticles with antimicrobial properties. TiO2 has great promise to diminish bacterial activity. Antimicrobial activity of TiO2and Ag-TiO2 nanoparticles against Escherichia coli was examined in this study. TiO2 nanoparticles with various silver contents were synthesized by sol gel method to produce uniform size, unagglomerated state and homogeneous nanoparticles. The nanoparticles were characterized by X-Ray diffraction (XRD) and transmittance electron microscopy (TEM). The effects of different silver concentration were studied using cotton diffusion test under fluorescence light irradiation. 0.06 mol % Ag-TiO2 revealed best antibacterial activity. 0.06 mol % Ag-TiO2 have antibacterial inhibition zone of 38 mm at the concentration of 2.0 M against E. coli. Swab test bacterial counts on left palm, tile, mouse pad and cotton have been tested before and after spraying with 0.06 mol % Ag-TiO2. It showed that the bacterial count decreased for entire samples. The significant enhancement in the antibacterial properties of Ag-TiO2 nanoparticles under visiblelight irradiation is related to the effect of noble metal Ag by acting as electron traps in TiO2 band gap. The phase structure, crystallite size and crystallinity of TiO2 also play an important role inantibacterial activity. The killing mechanism of Ag-TiO2 undervisible light irradiation antibacterial activity over Ag-TiO2nanoparticles was proposed based on our observations.

Garlic Extract (Allium sativum L.) Effectively Inhibits Staphylococcus aureus and Escherichia coli by Invitro Test

2020 ◽  
Vol 2 (2) ◽  
pp. 61-68
Author(s):  
Agnina Listya Anggraini ◽  
Ratih Dewi Dwiyanti ◽  
Anny Thuraidah
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Bacterial Infections ◽  
Allium Sativum ◽  
Antibacterial Properties ◽  
Herbal Medicines ◽  
Garlic Extract ◽  
Dilution Method ◽  
Initial Stage

Infection is a disease caused by the presence of pathogenic microbes, including Staphylococcus aureus and Escherichia coli. Garlic (Allium sativum L.) has chemical contents such as allicin, alkaloids, flavonoids, saponins, tannins, and steroids, which can function as an antibacterial against Staphylococcus aureus and Escherichia coli. This study aims to determine the antibacterial properties of garlic extract powder against Staphylococcus aureus and Escherichia coli. This research is the initial stage of the development of herbal medicines to treat Staphylococcus aureus and Escherichia coli infections. The antibacterial activity test was carried out by the liquid dilution method. The concentrations used were 30 mg/mL, 40 mg/mL, 50 mg/mL, 60 mg/mL and 70 mg/mL. The results showed that the Minimum Inhibitory Concentration (MIC) against Staphylococcus aureus and Escherichia coli was 40 mg/mL and 50 mg / mL. Minimum Bactericidal Concentration (MBC) results for Staphylococcus aureus and Escherichia coli are 50 mg/mL and 70 mg/mL. Based on the Simple Linear Regression test, the R2 value of Staphylococcus aureus and Escherichia coli is 0.545 and 0.785, so it can be concluded that there is an effect of garlic extract powder on the growth of Staphylococcus aureus and Escherichia coli by 54.5% and 78.5%. Garlic (Allium sativum L.) extract powder has potential as herbal medicine against bacterial infections but requires further research to determine its effect in vivo.

Fluoroquinolone-3-carboxamide Amino Acid Conjugates: Synthesis, Antibacterial Properties And Molecular Modeling Studies

2020 ◽  
Vol 17 (1) ◽  
pp. 71-84
Author(s):  
Riham M. Bokhtia ◽  
Siva S. Panda ◽  
Adel S. Girgis ◽  
Hitesh H. Honkanadavar ◽  
Tarek S. Ibrahim ◽  
...  
Keyword(s):  
Experimental Data ◽  
Antibacterial Activity ◽  
Amino Acid ◽  
Bacterial Infections ◽  
Antibacterial Agents ◽  
Antibacterial Properties ◽  
Computational Studies ◽  
Protecting Group ◽  
Amino Acid Conjugates ◽  
Molecular Modeling Studies

Background: Bacterial infections are considered as one of the major global health threats, so it is very essential to design and develop new antibacterial agents to overcome the drawbacks of existing antibacterial agents. Method: The aim of this work is to synthesize a series of new fluoroquinolone-3-carboxamide amino acid conjugates by molecular hybridization. We utilized benzotriazole chemistry to synthesize the desired hybrid conjugates. Result: All the conjugates were synthesized in good yields, characterized, evaluated for their antibacterial activity. The compounds were screened for their antibacterial activity using methods adapted from the Clinical and Laboratory Standards Institute. Synthesized conjugates were tested for activity against medically relevant pathogens; Escherichia coli (ATCC 25922), Pseudomonas aeruginosa (ATCC 27856) Staphylococcus aureus (ATCC 25923) and Enterococcus faecalis (ATCC 19433). Conclusion: The observed antibacterial experimental data indicates the selectivity of our synthesized conjugates against E.Coli. The protecting group on amino acids decreases the antibacterial activity. The synthesized conjugates are non-toxic to the normal cell lines. The experimental data were supported by computational studies.

scholarly journals Au–ZnO Conjugated Black Phosphorus as a Near-Infrared Light-Triggering and Recurrence-Suppressing Nanoantibiotic Platform against Staphylococcus aureus

Pharmaceutics ◽  
2021 ◽  
Vol 13 (1) ◽  
pp. 52
Author(s):  
Atanu Naskar ◽  
Sohee Lee ◽  
Kwang-sun Kim
Keyword(s):  
Staphylococcus Aureus ◽  
Bacterial Infections ◽  
Near Infrared ◽  
Au Nanoparticles ◽  
Synthesis Method ◽  
Antibacterial Properties ◽  
Black Phosphorus ◽  
Infrared Light ◽  
Resistant Bacteria ◽  
Near Infrared Light

Antibiotic therapy is the gold standard for bacterial infections treatment. However, the rapid increase in multidrug-resistant (MDR) bacterial infections and its recent use for secondary bacterial infections in many COVID-19 patients has considerably weakened its treatment efficacy. These shortcomings motivated researchers to develop new antibacterial materials, such as nanoparticle-based antibacterial platform with the ability to increase the chances of killing MDR strains and prevent their drug resistance. Herein, we report a new black phosphorus (BP)-based non-damaging near-infrared light-responsive platform conjugated with ZnO and Au nanoparticles as a synergistic antibacterial agent against Staphylococcus aureus species. First, BP nanosheets containing Au nanoparticles were assembled in situ with the ZnO nanoparticles prepared by a low-temperature solution synthesis method. Subsequently, the antibacterial activities of the resulting Au–ZnO–BP nanocomposite against the non-resistant, methicillin-resistant, and erythromycin-resistant S. aureus species were determined, after its photothermal efficacy was assessed. The synthesized nanocomposite exhibited excellent anti-S. aureus activity and good photothermal characteristics. The non-resistant S. aureus species did not produce drug-resistant bacteria after the treatment of multiple consecutive passages under the pressure of the proposed nanoantibiotic, but rapidly developed resistance to erythromycin. This work clearly demonstrates the excellent photothermal antibacterial properties of Au–ZnO–BP nanocomposite against the MDR S. aureus species.

Sign in / Sign up

Export Citation Format

Share Document