Characterization of Anaerobic Degradability and Kinetics of Harvested Submerged Aquatic Weeds Used for Nutrient Phytoremediation

Fuel particles for the High-Temperature Gas-Cooled Reactor (HTGR) contain a layer of pyrolytic silicon carbide to act as a miniature pressure vessel and primary fission product barrier. Optimization of the SiC with respect to fuel performance involves four areas of study: (a) characterization of as-deposited SiC coatings; (b) thermodynamics and kinetics of chemical reactions between SiC and fission products; (c) irradiation behavior of SiC in the absence of fission products; and (d) combined effects of irradiation and fission products. This paper reports the behavior of SiC deposited on inert microspheres and irradiated to fast neutron fluences typical of HTGR fuel at end-of-life.

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Elaboration and Characterization of the Properties of Refractory Cr Base Alloys

Advances in Science and Technology ◽

10.4028/www.scientific.net/ast.72.46 ◽

2010 ◽

Vol 72 ◽

pp. 46-52 ◽

Cited By ~ 1

Author(s):

Laurent Royer ◽

Stéphane Mathieu ◽

Christophe Liebaut ◽

Pierre Steinmetz

Keyword(s):

Mechanical Properties ◽

Melting Point ◽

Molten Glass ◽

Energy Production ◽

Creep Properties ◽

Refractory Alloys ◽

Industrial Use ◽

Kinetics Of ◽

Selection Of

For energy production and also for the glass industry, finding new refractory alloys which could permit to increase the process temperatures to 1200°C or more is a permanent challenge. Chromium base alloys can be good candidates, considering the melting point of Cr itself, and also its low corrosion rate in molten glass. Two families of alloys have been studied for this purpose, Cr-Mo-W and Cr-Ta-X alloys (X= Mo, Si..). A finer selection of compositions has been done, to optimize their chemical and mechanical properties. Kinetics of HT oxidation by air, of corrosion by molten glass and also creep properties of several alloys have been measured up to 1250°C. The results obtained with the best alloys (Cr-Ta base) give positive indications as regards the possibility of their industrial use.

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New Trimethoprim-Like Molecules: Bacteriological Evaluation and Insights into Their Action

Antibiotics ◽

10.3390/antibiotics10060709 ◽

2021 ◽

Vol 10 (6) ◽

pp. 709

Author(s):

Marta Jorba ◽

Marina Pedrola ◽

Ouldouz Ghashghaei ◽

Rocío Herráez ◽

Lluis Campos-Vicens ◽

...

Keyword(s):

Inhibitory Concentration ◽

Efflux Pump ◽

Synergistic Effects ◽

Nicotinamide Adenine Dinucleotide Phosphate ◽

Detailed Characterization ◽

Low Concentrations ◽

Pump Activity ◽

Bacteriological Evaluation ◽

Kinetics Of

This work reports a detailed characterization of the antimicrobial profile of two trimethoprim-like molecules (compounds 1a and 1b) identified in previous studies. Both molecules displayed remarkable antimicrobial activity, particularly when combined with sulfamethoxazole. In disk diffusion assays on Petri dishes, compounds 1a and 1b showed synergistic effects with colistin. Specifically, in combinations with low concentrations of colistin, very large increases in the activities of compounds 1a and 1b were determined, as demonstrated by alterations in the kinetics of bacterial growth despite only slight changes in the fractional inhibitory concentration index. The effect of colistin may be to increase the rate of antibiotic entry while reducing efflux pump activity. Compounds 1a and 1b were susceptible to extrusion by efflux pumps, whereas the inhibitor phenylalanine arginyl β-naphthylamide (PAβN) exerted effects similar to those of colistin. The interactions between the target enzyme (dihydrofolate reductase), the coenzyme nicotinamide adenine dinucleotide phosphate (NADPH), and the studied molecules were explored using enzymology tools and computational chemistry. A model based on docking results is reported.

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Simultaneous Kinetics of Selenite Oxidation and Sorption on δ-MnO2 in Stirred-Flow Reactors

International Journal of Environmental Research and Public Health ◽

10.3390/ijerph18062902 ◽

2021 ◽

Vol 18 (6) ◽

pp. 2902

Author(s):

Zheyong Li ◽

Yajun Yuan ◽

Lin Ma ◽

Yihui Zhang ◽

Hongwei Jiang ◽

...

Keyword(s):

Rate Constant ◽

Photoelectron Spectroscopy ◽

Oxide Surfaces ◽

Kinetic Rate Constant ◽

Flow Reactors ◽

Mn Oxide ◽

Remediation Strategies ◽

Process Of Se ◽

Kinetics Of

Selenium (Se) is an essential and crucial micronutrient for humans and animals, but excessive Se brings negativity and toxicity. The adsorption and oxidation of Se(IV) on Mn-oxide surfaces are important processes for understanding the geochemical fate of Se and developing engineered remediation strategies. In this study, the characterization of simultaneous adsorption, oxidation, and desorption of Se(IV) on δ-MnO2 mineral was carried out using stirred-flow reactors. About 9.5% to 25.3% of Se(IV) was oxidized to Se(VI) in the stirred-flow system in a continuous and slow process, with the kinetic rate constant k of 0.032 h−1, which was significantly higher than the apparent rate constant of 0.0014 h−1 obtained by the quasi-level kinetic fit of the batch method. The oxidation reaction was driven by proton concentration, and its rate also depended on the Se(IV) influent concentration, flow rate, and δ-MnO2 dosage. During the reaction of Se(IV) and δ-MnO2, Mn(II) was produced and adsorbed strongly on Mn oxide surfaces, which was evidenced by the total reflectance Fourier transform infrared (ATR-FTIR) results. The X-ray photoelectron spectroscopy (XPS) data indicated that the reaction of Se(VI) on δ-MnO2 produced Mn(III) as the main product. These results contribute to a deeper understanding of the interface chemical process of Se(IV) with δ-MnO2 in the environment.

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Synthesis and Polymerization Kinetics of Rigid Tricyanate Ester

Polymers ◽

10.3390/polym13111686 ◽

2021 ◽

Vol 13 (11) ◽

pp. 1686

Author(s):

Andrey Galukhin ◽

Roman Nosov ◽

Ilya Nikolaev ◽

Elena Melnikova ◽

Daut Islamov ◽

...

Keyword(s):

Kinetic Analysis ◽

Single Step ◽

Polymerization Kinetics ◽

Scanning Calorimetry ◽

Modulated Differential Scanning Calorimetry ◽

Diffusion Controlled ◽

Polymerization Product ◽

The One ◽

Kinetics Of

A new rigid tricyanate ester consisting of seven conjugated aromatic units is synthesized, and its structure is confirmed by X-ray analysis. This ester undergoes thermally stimulated polymerization in a liquid state. Conventional and temperature-modulated differential scanning calorimetry techniques are employed to study the polymerization kinetics. A transition of polymerization from a kinetic- to a diffusion-controlled regime is detected. Kinetic analysis is performed by combining isoconversional and model-based computations. It demonstrates that polymerization in the kinetically controlled regime of the present monomer can be described as a quasi-single-step, auto-catalytic, process. The diffusion contribution is parameterized by the Fournier model. Kinetic analysis is complemented by characterization of thermal properties of the corresponding polymerization product by means of thermogravimetric and thermomechanical analyses. Overall, the obtained experimental results are consistent with our hypothesis about the relation between the rigidity and functionality of the cyanate ester monomer, on the one hand, and its reactivity and glass transition temperature of the corresponding polymer, on the other hand.

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Biosorption and Diffusion Modeling of Pb(II) by Malt Bagasse

International Journal of Chemical Engineering ◽

10.1155/2016/4210561 ◽

2016 ◽

Vol 2016 ◽

pp. 1-11 ◽

Cited By ~ 4

Author(s):

Klaiani B. Fontana ◽

Giane Gonçalves Lenzi ◽

Erica R. L. R. Watanabe ◽

Ervin Kaminski Lenzi ◽

Juliana A. M. T. Pietrobelli ◽

...

Keyword(s):

Sorption Process ◽

Isotherm Models ◽

Diffusion Modeling ◽

Biosorption Capacity ◽

Kinetic And Thermodynamic Parameters ◽

Pseudo Second Order ◽

Ph Optimization ◽

Kinetics Of ◽

And Diffusion

The removal of Pb(II) from water by biosorption processes onto malt bagasse was investigated and the kinetic and thermodynamic parameters were obtained; additionally a diffusion modeling was proposed. The characterization of malt bagasse was performed by FTIR and SEM/EDS. The experiments were conducted in batch system and an experimental design based response surface methodology was applied for agitation speed and pH optimization. The kinetics of biosorption followed pseudo-second-order model and the temperature of the process affected the biosorption capacity. Isotherm models of Langmuir, Freundlich, and Elovich were applied and the Langmuir model showed better fit and the estimated biosorption capacity was 29.1 mg g−1. The negative values obtained for ΔG° and positive values of ΔH° confirm, respectively, the spontaneous and endothermic nature of the process. The diffusion modeling was performed based on experiments in the absence of agitation to investigate the influence of the biosorbent on the sorption process of Pb(II) ions.

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High-resolution view of HIV-1 reverse transcriptase initiation complexes and inhibition by NNRTI drugs

Nature Communications ◽

10.1038/s41467-021-22628-9 ◽

2021 ◽

Vol 12 (1) ◽

Author(s):

Betty Ha ◽

Kevin P. Larsen ◽

Jingji Zhang ◽

Ziao Fu ◽

Elizabeth Montabana ◽

...

Keyword(s):

Reverse Transcriptase ◽

Viral Entry ◽

Reverse Transcription ◽

Molecular Mechanisms ◽

Dissociation Kinetics ◽

Structural Mechanism ◽

Medium Resolution ◽

Kinetics Of ◽

Hiv 1

AbstractReverse transcription of the HIV-1 viral RNA genome (vRNA) is an integral step in virus replication. Upon viral entry, HIV-1 reverse transcriptase (RT) initiates from a host tRNALys3 primer bound to the vRNA genome and is the target of key antivirals, such as non-nucleoside reverse transcriptase inhibitors (NNRTIs). Initiation proceeds slowly with discrete pausing events along the vRNA template. Despite prior medium-resolution structural characterization of reverse transcriptase initiation complexes (RTICs), higher-resolution structures of the RTIC are needed to understand the molecular mechanisms that underlie initiation. Here we report cryo-EM structures of the core RTIC, RTIC–nevirapine, and RTIC–efavirenz complexes at 2.8, 3.1, and 2.9 Å, respectively. In combination with biochemical studies, these data suggest a basis for rapid dissociation kinetics of RT from the vRNA–tRNALys3 initiation complex and reveal a specific structural mechanism of nucleic acid conformational stabilization during initiation. Finally, our results show that NNRTIs inhibit the RTIC and exacerbate discrete pausing during early reverse transcription.

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Biochar Chemistry in a Weathered Tropical Soil: Kinetics of Phosphorus Sorption

Agriculture ◽

10.3390/agriculture11040295 ◽

2021 ◽

Vol 11 (4) ◽

pp. 295

Author(s):

Marina Moura Morales ◽

Nicholas Brian Comerford ◽

Maurel Behling ◽

Daniel Carneiro de Abreu ◽

Iraê Amaral Guerrini

Keyword(s):

Mineral Soil ◽

Soil Surface ◽

Kinetic Studies ◽

Inorganic Phosphorus ◽

Initial Reaction ◽

Phosphorus Sorption ◽

P Sorption ◽

Published Research ◽

Kinetics Of

The phosphorus (P) chemistry of biochar (BC)-amended soils is poorly understood. This statement is based on the lack of published research attempting a comprehensive characterization of biochar’s influence on P sorption. Therefore, this study addressed the kinetic limitations of these processes. This was accomplished using a fast pyrolysis biochar made from a mix of waste materials applied to a highly weathered Latossolo Vermelho distrofico (Oxisol) from São Paulo, Brazil. Standard method (batch method) was used. The sorption kinetic studies indicated that P sorption in both cases, soil (S) and soil-biochar (SBC), had a relatively fast initial reaction between 0 to 5 min. This may have happened because adding biochar to the soil decreased P sorption capacity compared to the mineral soil alone. Presumably, this is a result of: (i) Inorganic phosphorus desorbed from biochar was resorbed onto the mineral soil; (ii) charcoal particles physically covered P sorption locations on soil; or (iii) the pH increased when BC was added SBC and the soil surface became more negatively charged, thus increasing anion repulsion and decreasing P sorption.

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KD determination from time-resolved experiments on live cells with LigandTracer and reconciliation with end-point flow cytometry measurements

European Biophysics Journal ◽

10.1007/s00249-021-01560-2 ◽

2021 ◽

Author(s):

Diana Spiegelberg ◽

Jonas Stenberg ◽

Pascale Richalet ◽

Marc Vanhove

Keyword(s):

Flow Cytometry ◽

Live Cells ◽

Time Resolved ◽

Surface Receptors ◽

Full Characterization ◽

End Point ◽

Titration Curves ◽

Kinetics Of

AbstractDesign of next-generation therapeutics comes with new challenges and emulates technology and methods to meet them. Characterizing the binding of either natural ligands or therapeutic proteins to cell-surface receptors, for which relevant recombinant versions may not exist, represents one of these challenges. Here we report the characterization of the interaction of five different antibody therapeutics (Trastuzumab, Rituximab, Panitumumab, Pertuzumab, and Cetuximab) with their cognate target receptors using LigandTracer. The method offers the advantage of being performed on live cells, alleviating the need for a recombinant source of the receptor. Furthermore, time-resolved measurements, in addition to allowing the determination of the affinity of the studied drug to its target, give access to the binding kinetics thereby providing a full characterization of the system. In this study, we also compared time-resolved LigandTracer data with end-point KD determination from flow cytometry experiments and hypothesize that discrepancies between these two approaches, when they exist, generally come from flow cytometry titration curves being acquired prior to full equilibration of the system. Our data, however, show that knowledge of the kinetics of the interaction allows to reconcile the data obtained by flow cytometry and LigandTracer and demonstrate the complementarity of these two methods.

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Characterization of the kinetics of breakdown of protein stabilized oil in water emulsions

Journal of Agricultural and Food Chemistry ◽

10.1021/jf00087a006 ◽

1989 ◽

Vol 37 (3) ◽

pp. 600-604 ◽

Cited By ~ 6

Author(s):

Robert L. Jackman ◽

Rickey Y. Yada ◽

Allan T. Paulson

Keyword(s):

Oil In Water ◽

Kinetics Of

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