scholarly journals Bacteriocinogenic Bacillus spp. Isolated from Korean Fermented Cabbage (Kimchi)—Beneficial or Hazardous?

Fermentation ◽  
2021 ◽  
Vol 7 (2) ◽  
pp. 56
Author(s):  
Joanna Ivy Irorita Fugaban ◽  
Jorge Enrique Vazquez Bucheli ◽  
Wilhelm Heinrich Holzapfel ◽  
Svetoslav Dimitrov Todorov
Keyword(s):  
Antimicrobial Activity ◽  
Enzyme Production ◽  
Proteolytic Enzymes ◽  
Activity Levels ◽  
Bacillus Velezensis ◽  
Gelatinase Activity ◽  
Bacillus Spp ◽  
Inhibition Zones ◽  
Antimicrobial Metabolites ◽  
Positive Results

Bacillus velezensis ST03 and ST32, Bacillus amyloliquefaciens ST06 and ST109, and Bacillus subtilis ST08 were isolated from artisanal-produced kimchi and were identified based on 16S rRNA partial sequencing. DNA obtained from the investigated bacilli generated positive results for lichenicidin, iturin, subtilosin, and surfactin on a strain-specific basis. The strains were found to produce antimicrobial metabolites with activity levels ranging between 800 and 1600 AU/mL on a strain-specific basis, as determined against Listeria monocytogenes ATCC15313. Moreover, all tested strains in this study were still active after treatment with proteolytic enzymes, even with reduced inhibition zones compared to the controls, pointing to additional antimicrobial activity possibly related to a non-proteinaceous molecular structure. Most probably these strains may express surfactin as an additional factor in their complex antimicrobial activity. B. amyloliquefaciens ST09 and B. velezensis ST03 and ST32 were characterized as positive for β-hemolysis. B. subtilis ST08 was shown to be positive for hblC and nheC and B. amyloliquefaciens ST109 for nheB. B. amyloliquefaciens ST109 generated positive results for gelatinase activity. The ability of the studied Bacillus strains to metabolize different carbohydrate sources was done based on the API50CHB test, while the enzyme production profile was recorded by the APIZym kit. All studied strains were positive producers for biogenic amines production. Studied Bacillus spp. strains were resistant to some of the evaluated antibiotics, tested according to recommendations of CLSI and EFSA.

scholarly journals Selection of Bacteriocinogenic Bacillus spp. from Traditional Fermented Korean Food Products with Additional Beneficial Properties

Fermentation ◽  
2021 ◽  
Vol 7 (4) ◽  
pp. 271
Author(s):  
Gee Hyeun Choi ◽  
Joanna Ivy Irorita Fugaban ◽  
Clarizza May Dioso ◽  
Jorge Enrique Vazquez Bucheli ◽  
Wilhelm Heinrich Holzapfel ◽  
...  
Keyword(s):  
Proteolytic Enzymes ◽  
Antimicrobial Properties ◽  
Antimicrobial Effect ◽  
Penicillin G ◽  
Related Factors ◽  
Antimicrobial Substances ◽  
Different Temperatures ◽  
Health Related ◽  
Bacillus Spp ◽  
Antimicrobial Metabolites

Two Bacillus spp. isolated from kimchi, Bacillus tequilensis ST816CD and Bacillus subtilis ST830CD, were characterized for their antimicrobial properties and safety. The proteinaceous nature of their inhibitory metabolites was confirmed after exposure to proteolytic enzymes, resulting in partial loss of the antimicrobial effect. This indicated that different non-proteinaceous antimicrobial substances may also be produced by these strains. This hypothesis was later confirmed when genes associated with the production of surfactants were detected in their DNA. The expressed antimicrobial metabolites were not affected by treatment at different temperatures and pH levels, including exposure to selected chemicals. Their strong adherence to susceptible pathogens was not significantly affected by different temperatures, chemicals, or pH values. Both Bacillus strains showed inhibitory activity against clinical and food-associated pathogens, including Listeria monocytogenes ATCC 15313, and some Staphylococcus species. Several genes associated with the production of antimicrobial metabolites were detected, but key virulence and beneficial genes were not present in these strains. Even though only B. tequilensis ST816CD displayed γ-hemolysin production, both selected strains were found to produce gelatinase and biogenic amines, which are considered as either potential virulence- or health-related factors. Moreover, the strains were susceptible to a variety of antibiotics except for the penicillin G [1 IU/disc] resistance of B. tequilensis ST816CD. Both strains showed proteolytic activity. Additionally, both strains showed low hydrophobicity based on bacterial adherence measured by hydrocarbons (n-hexadecane).

scholarly journals Draft Genome Sequence of Bacillus velezensis OSY-GA1, Which Encodes Multiple Antimicrobial Metabolites and Expresses Antimicrobial Activity against Foodborne Pathogens

2019 ◽  
Vol 8 (7) ◽  
Author(s):  
Ahmed G. Abdelhamid ◽  
Walaa E. Hussein ◽  
Michelle M. Gerst ◽  
Ahmed E. Yousef
Keyword(s):  
Antimicrobial Activity ◽  
Genome Sequence ◽  
Foodborne Pathogens ◽  
Draft Genome ◽  
Soil Bacterium ◽  
Bacillus Velezensis ◽  
Gram Positive ◽  
Gram Negative ◽  
Content Type ◽  
Antimicrobial Metabolites

Bacillus velezensis OSY-GA1 is a Gram-positive soil bacterium that exhibits antagonistic activities against Gram-positive and Gram-negative foodborne pathogens. Here, we present the strain’s draft genome, which is 4,009,999 bp long with an average G+C content of 46.2%.

scholarly journals Antimicrobial Metabolites against Methicillin-Resistant Staphylococcus aureus from the Endophytic Fungus Neofusicoccum australe

Molecules ◽  
2021 ◽  
Vol 26 (4) ◽  
pp. 1094
Author(s):  
Melissa M. Cadelis ◽  
Soeren Geese ◽  
Benedict B. Uy ◽  
Daniel R. Mulholland ◽  
Shara J. van de Pas ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Antimicrobial Activity ◽  
Natural Products ◽  
Structure Elucidation ◽  
Methicillin Resistant Staphylococcus Aureus ◽  
Inhibitory Concentration ◽  
Spectroscopic Methods ◽  
Methicillin Resistant ◽  
Bioassay Guided Fractionation ◽  
Antimicrobial Metabolites

Antimicrobial bioassay-guided fractionation of the endophytic fungi Neofusicoccum australe led to the isolation of a new unsymmetrical naphthoquinone dimer, neofusnaphthoquinone B (1), along with four known natural products (2–5). Structure elucidation was conducted by nuclear magnetic resonance (NMR) spectroscopic methods, and the antimicrobial activity of all the natural products was investigated, revealing 1 to be moderately active towards methicillin-resistant Staphylococcus aureus (MRSA) with a minimum inhibitory concentration (MIC) of 16 µg/mL.

scholarly journals Evaluation of antimicrobial activity of the endophytic actinomycete R18(6) against multiresistant Gram-negative bacteria

2016 ◽  
Vol 88 (1) ◽  
pp. 155-163 ◽  
Author(s):  
Tiele Carvalho ◽  
Sueli Van Der Sand
Keyword(s):  
Antimicrobial Activity ◽  
Inhibitory Concentration ◽  
Proteolytic Enzymes ◽  
Gram Negative Bacteria ◽  
Bacteriostatic Activity ◽  
Submerged Cultures ◽  
Gram Negative ◽  
Endophytic Actinomycete ◽  
Endophytic Actinomycetes ◽  
Antimicrobial Substances

Endophytic actinomycetes are promising sources of antimicrobial substances. This study evaluates the activity of metabolites produced by the endophytic actinomycete R18(6) against Gram-negative bacteria multiresistant to antimicrobials. R18(6) isolate was grown in submerged cultures under different conditions: carbon source, temperature, pH and incubation time to optimize antimicrobials production. The actinomycete grown in base medium supplemented with 1% glucose, pH 6.5 and incubation at 30 ºC for 96 h with shaking at 100 rpm, exhibited the highest activity against the used Gram-negative bacteria. Minimum inhibitory concentration (MIC) of the crude extract produced by the microorganism varied between 1/32 and 1/256. It had bactericide or bacteriostatic activity, depending on the Gram-negative organism. The active extract was stable at high temperatures, and unstable in medium containing proteolytic enzymes. Micromorphology of R18(6) was investigated by optical and scan microscopy, revealing that it was morphologically similar to the genusStreptomyces.

Biosynthesis of Silver Nanoparticles by Punica Granatum Peel Extract and their Biological Activity on different Pathogenic Bacteria

2021 ◽  
Vol 19 (9) ◽  
pp. 38-45
Author(s):  
Hussein H. Al-Turnachy ◽  
Fadhilk. alibraheemi ◽  
Ahmed Abd Alreda Madhloom ◽  
Zahraa Yosif Motaweq ◽  
Nibras Yahya Abdulla
Keyword(s):  
Antimicrobial Activity ◽  
Silver Nanoparticles ◽  
Biological Activity ◽  
Pathogenic Bacteria ◽  
Punica Granatum ◽  
Gram Positive ◽  
Gram Negative ◽  
Inhibition Zones ◽  
Peel Extract

The present study was included the assessment of the antimicrobial activity of AgNPs synthesized by Punica granatum peel extract against pathogenic bacteria by testing warm aqueous P. granatum peel extract and silver nanoparticles. Punica granatum indicated potency for AgNP extracellular nanobiosynthesis after addition of silver nitrate (AgNO3) 4mM to the extract supernatant, in both concentrations (100mg and 50mg). The biogenic AgNPs showed potency to inhibit both gram-negative and gram-positive bacterial growth. Zons of inhibition in (mm) was lesser in gram-positive than gram-negative bacteria. The resulted phytogenic AgNPs gave higher biological activity than warm aqueous Punica granatum peel extract. The inhibition zone of the phytogenic AgNPs on E. coli reached 17.53, 22.35, and 26.06 mm at (0.1, 0.5, and 1) mg/ml respectively. While inhibition zones of Punica warm aqueous extract reached 5.33, 10.63, and 16.08 mm at the same concentrations. phytogenic AgNPs gave smaller inhibition zones in gram-positive than gram- negative. Cytotoxic activity of the phytogenic AgNPs was assayed in vitro agaist human blood erythrocytes (RBCs), spectroscopic results showed absorbance at 540 nm hemolysis was observed. In general, AgNPs showed least RBCs hemolysis percentage, at 1 mg/ml concentration, hemolysis percentage was (4.50%). This study, concluded that the Punica granatum peel extract has the power of synthses of AgNPs characterized by broad spectrum antimicrobial activity with cyto-toxicity proportional to AgNPs concentration.

scholarly journals Synthesis of Silver Nano Particles from Adansonia digitata Leaf Extract and Its Antimicrobial Properties

2020 ◽  
pp. 10-22
Author(s):  
A. O. Akintola ◽  
A. M. Azeez ◽  
B. D. Kehinde ◽  
I. C. Oladipo
Keyword(s):  
Staphylococcus Aureus ◽  
Antimicrobial Activity ◽  
Aspergillus Niger ◽  
Pathogenic Bacteria ◽  
Leaf Extract ◽  
Biomedical Application ◽  
Nano Particles ◽  
Diffusion Method ◽  
Adansonia Digitata ◽  
Inhibition Zones

Silver nano particles (AgNPs) were green synthesized using Adansonia digitata leaf extract. The synthesized silver nano particles were characterized in terms of synthesis, size, shape, morphology and capping functionalities by UV-Visible Spectroscopy, Scanning Electron Microscopy (SEM) and Fourier Transform Infrared Spectroscopy (FTIR). Antimicrobial activity of the synthesized silver nano particles was investigated by well diffusion method. The antibacterial activity of the nano particle was studied against Staphylococcus aureus, Escherichia coli, Bacillus subtilis, Pseudomonas aeurigunosa, Salmonella typhi and Klebsiella pneumonae while the antifungal activity was studied against Candida albicans, Aspergillus niger, Penicillum notatum and Rhizopus stolomifer. The synthesized AgNPs was active against all the studied microorganisms. Staphylococcus aureus was the most susceptible bacterium (inhibition zones ranging from 12.00 to 28.00 mm, MIC: 30 µl, MBC: 50 µl) while Aspergillus niger was the most susceptible fungi (inhibition zones ranging from 10.00 to 18.00 mm, MIC: 90 µl, MFC: 120 µl. In conclusion the synthesized silver nanoparticles was found to have antimicrobial activity against the pathogenic bacteria and fungi tested and hence has a great potential in biomedical application for the treatment of microbial infections.

scholarly journals Enhanced Control of Plant Wilt Disease by a Xylose-Inducible degQ Gene Engineered into Bacillus velezensis Strain SQR9XYQ

2019 ◽  
Vol 109 (1) ◽  
pp. 36-43 ◽  
Author(s):  
Zhihui Xu ◽  
Jiyu Xie ◽  
Huihui Zhang ◽  
Dandan Wang ◽  
Qirong Shen ◽  
...  
Keyword(s):  
Plant Growth ◽  
Biofilm Formation ◽  
Antibiotic Production ◽  
Genetically Engineered ◽  
Flagellar Motility ◽  
Bacillus Velezensis ◽  
Tomato Root ◽  
Bacillus Spp ◽  
Plant Growth Promoting

Bacillus velezensis SQR9 (former B. amyloliquefaciens SQR9) is a plant-growth-promoting rhizobacterium (PGPR) that promotes plant growth and health. The colonization of PGPR strains along plant roots is a prerequisite for them to execute their specific functions. However, one problem of microbial introduction in practice is that the applied PGPR strains do not always successfully colonize the rhizosphere. In Bacillus spp., two-component signal transduction system (TCS) DegS/U regulates flagellar motility, biofilm formation and antibiotic production. Phosphorylation of DegU by DegS is positively affected by DegQ protein. In this study, we constructed a xylose-inducible degQ genetically engineered strain SQR9XYQ to improve the biocontrol activity. The results from in vitro, root in situ, greenhouse experiments and RT-qPCR studies demonstrate that (i) the phosphorylation of DegU in SQR9XYQ can be gradually activated by xylose, which is a component of both cucumber and tomato root exudates, and (ii) biofilm formation, antibiotic expression, colonization activity, and biocontrol efficiency were improved in SQR9XYQ compared with the wild-type strain SQR9. These results suggest that colonization trait is important to biocontrol strains for maintenance of plant health.

A Colony Blot Immunoassay for the Rapid Identification of Bacillus cereus

2004 ◽  
Vol 67 (2) ◽  
pp. 387-390 ◽  
Author(s):  
CHI-HUA CHEN ◽  
HWIA CHENG DING
Keyword(s):  
Horseradish Peroxidase ◽  
Cell Surface ◽  
Bacillus Cereus ◽  
False Positive ◽  
Rapid Identification ◽  
True Negative ◽  
Negative Results ◽  
Bacillus Spp ◽  
Positive Results ◽  
Peroxidase Conjugate

A colony blot immunoassay was developed for the rapid identification of Bacillus cereus using antibodies against the 28.5-kDa cell-surface antigen of B. cereus. Suspect colonies from plates were blotted onto a Whatman #541 membrane, dried, and fixed by UV irradiation. The membrane was then immersed in an anti- B. cereus antibody-horseradish peroxidase conjugate for 60 min. After washing and reacting with 4-chloro-1-naphthol and H2O2, the appearance of purple spots indicated the presence of B. cereus. This assay effectively identified 61 of 62 B. cereus strains tested. Among 38 non– B. cereus strains, which were other Bacillus spp. (19 genera), 36 gave true-negative results, and 2 showed false-positive results. The sensitivity and specificity for B. cereus were 98.4 and 94.7%, respectively. The present assay is easy to use, and the rapid identification of B. cereus can be completed in 2.5 h.

scholarly journals Chemical Composition and Antimicrobial Activity of Clausena Indica (Dalz) Oliv. (Rutaceae) Essential Oil from Vietnam

2009 ◽  
Vol 4 (6) ◽  
pp. 1934578X0900400 ◽  
Author(s):  
Pham Thi Minh Diep ◽  
Agata Maria Pawlowska ◽  
Pier Luigi Cioni ◽  
Chau Van Minh ◽  
Le Mai Huong ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Chemical Composition ◽  
Essential Oil ◽  
Bacterial Strains ◽  
Positive Results ◽  
Three Components

The essential oil obtained from the branches and leaves of Clausena indica (Dalz) Oliv. (Rutaceae) has been analyzed by GC/MS. Fifty-three components of the essential oil, representing 96.9% of the total amount, were identified. The main constituents were myristicin (35.3%), terpinolene (16.7%), and δ-3-carene (11.3%). The essential oil was screened for antimicrobial activity, showing positive results against some bacterial strains. Moreover, a comparison between constituents of C. indica essential oil with the ones reported in the literature for other Clausena ssp. is pointed out and some chemotaxonomic considerations have been identified.

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