scholarly journals Citrus Peel Extracts for Industrial-Scale Production of Bio-Based Active Food Packaging

Foods ◽  
2021 ◽  
Vol 11 (1) ◽  
pp. 30
Author(s):  
Cecilia Fiorentini ◽  
Guillermo Duserm Duserm Garrido ◽  
Andrea Bassani ◽  
Claudia Cortimiglia ◽  
Marta Zaccone ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Antimicrobial Activity ◽  
Food Packaging ◽  
Industrial Scale ◽  
Total Phenolic ◽  
Scale Production ◽  
Citrus Peel ◽  
Active Food Packaging ◽  
Food Simulant ◽  
Encapsulation Process

The thermal stability of four different commercial citrus peel extracts was tested and improved by an encapsulation process with β-cyclodextrins in a spray-dryer. All extracts after the encapsulation process maintained a good antioxidant capacity, with an apparent loss in total phenolic compounds of around 20–25%. In addition, all samples showed good antimicrobial activity (MIC 5-0.625 mg/mL) against Staphylococcus aureus, which was maintained after the encapsulation process (MIC 5-1.25 mg/mL). Based on the antioxidant and antimicrobial activity results, the best-encapsulated citrus extract was selected for incorporation into a polylactic acid/polyhydroxy butyrate (PLA/PHB) film. The latter was then produced on an industrial scale by cast extrusion and was found to be suitable for food contact as it showed overall migration values in different food simulants lower than the legislative limit of 10 mg of non-volatile substances per 1 dm2 of surface area. The UHPLC-HRMS analysis, performed to evaluate the migration of the active compounds, revealed about 13.41% release in food simulant A and 11.02% in food simulant B. Antimicrobial analysis conducted directly on the film showed a growth inhibition activity towards Escherichia coli and Staphylococcus aureus equal to 30 and 60%, respectively.

scholarly journals Incorporation of Poly(Itaconic Acid) with Quaternized Thiazole Groups on Gelatin-Based Films for Antimicrobial-Active Food Packaging

Polymers ◽  
2021 ◽  
Vol 13 (2) ◽  
pp. 200
Author(s):  
Celeste Cottet ◽  
Andrés G. Salvay ◽  
Mercedes A. Peltzer ◽  
Marta Fernández-García
Keyword(s):  
Antimicrobial Activity ◽  
Itaconic Acid ◽  
Food Packaging ◽  
Crosslinking Agent ◽  
Radical Scavenging ◽  
Proton Nuclear Magnetic Resonance ◽  
Esterification Reaction ◽  
Alkylation Reaction ◽  
Vapor Permeability ◽  
Active Food Packaging

Poly(itaconic acid) (PIA) was synthesized via conventional radical polymerization. Then, functionalization of PIA was carried out by an esterification reaction with the heterocyclic groups of 1,3-thiazole and posterior quaternization by N-alkylation reaction with iodomethane. The modifications were confirmed by Fourier transform infrared (FTIR) and proton nuclear magnetic resonance (1H-NMR), as well as ζ-potential measurements. Their antimicrobial activity was tested against different Gram-negative and Gram-positive bacteria. After characterization, the resulting polymers were incorporated into gelatin with oxidized starch and glycerol as film adjuvants, and dopamine as crosslinking agent, to develop antimicrobial-active films. The addition of quaternized polymers not only improved the mechanical properties of gelatin formulations, but also decreased the solution absorption capacity during the swelling process. However, the incorporation of synthesized polymers increased the deformation at break values and the water vapor permeability of films. The antioxidant capacity of films was confirmed by radical scavenging ability and, additionally, those films exhibited antimicrobial activity. Therefore, these films can be considered as good candidates for active packaging, ensuring a constant concentration of the active compound on the surface of the food, increasing products’ shelf-life and reducing the environmental impact generated by plastics of petrochemical origin.

scholarly journals Antioxidant, Antibacterial Analysis of Pectin Isolated from Banana Peel and its Application in Edible Coating of Freshly Made Mozzarella Cheese

2021 ◽  
pp. 82-92
Author(s):  
Srishti Tripathi ◽  
Sunita Mishra
Keyword(s):  
Staphylococcus Aureus ◽  
Antioxidant Activity ◽  
Shelf Life ◽  
Food Packaging ◽  
Salmonella Enteritidis ◽  
Diffusion Method ◽  
Banana Peel ◽  
Total Phenolic ◽  
Mozzarella Cheese

The present study aimed to evaluate the antibacterial, antioxidant activity of pectin extracted from banana peel. Antibacterial activity was investigated against Staphylococcus aureus, Escherichia coli, and Salmonella Enteritidis. The well diffusion method was used to assess the antibacterial effect of the pectin extract on microorganisms. The extract showed maximum activity against Staphylococcus aureus (19.6 mm). The total phenolic content and flavonoid content in the examined extract found to be 3883.6 mgGA/g and 903.03 mg QE/gm on a dry matter basis. Antioxidant activity is analyzed using in vitro Standard spectrophotometer methods. Pectin extract increases DPPH scavenging activity up to 75 µg/ml of concentration. The innovation in food packaging by the use of pectin-based edible coatings is reviewed in this paper. Thereafter, coating of pectin was done in mozzarella cheese and its shelf life was studied at 1, 7,14,21, and 28 days of storage at 5˚C. It was analyzed that pectin coating over mozzarella cheese increases their shelf life from 7 to 21 days. Thus, pectin is a natural polysaccharide that attracts interest for maintaining and improving the quality of cheese. Also, it minimizes the waste that occurs from non-biodegradable packaging materials and helps the environment to be safe. This research was carried out at the laboratory of Food Science analysis Laboratory, Babasaheb Bhimrao Ambedkar University, Lucknow (INDIA) between February 21-April 21.

scholarly journals Active Packaging—Poly(Vinyl Alcohol) Films Enriched with Tomato By-Products Extract

Coatings ◽  
2020 ◽  
Vol 10 (2) ◽  
pp. 141 ◽  
Author(s):  
Katalin Szabo ◽  
Bernadette-Emoke Teleky ◽  
Laura Mitrea ◽  
Lavinia-Florina Călinoiu ◽  
Gheorghe-Adrian Martău ◽  
...  
Keyword(s):  
Vinyl Alcohol ◽  
Total Phenolic Content ◽  
Food Packaging ◽  
Phenolic Content ◽  
Flow Behavior ◽  
Poly Vinyl Alcohol ◽  
Total Phenolic ◽  
Active Food Packaging ◽  
Film Forming ◽  
By Products

Active films were prepared from poly(vinyl alcohol) (PVA) blended with itaconic acid (Ia), and with chitosan (Ch), enriched with tomato processing by-products extract (TBE) in order to develop new bioactive formulations for food packaging. The effects of two biopolymers (Ch, Ia) and of the incorporated TBE—containing phenolic compounds and carotenoids—were studied regarding the physical and antimicrobial properties of films; in addition, their influence on the total phenolic content, viscosity, and flow behavior on the film-forming solutions was investigated. The results showed increased physical properties (diameter, thickness, density, weight) of the films containing the TBE versus their control. TBE and Ch conferred significant antimicrobial effects to PVA films toward all the tested microorganisms, whereas the best inhibition was registered against S. aureus and P. aeruginosa, with a minimum inhibitory concentration of <0.078 mg DW/mL. The Ia-PVA films also exhibited some antibacterial activity against P. aeruginosa (2.5 mg DW/mL). The total phenolic content of the film-forming solutions presented the highest values for the TBE and Ch-added PVA samples (0.208 mg gallic acid/100 mL film-forming solution). These results suggest that the PVA + Ch film containing TBE can be used for the development of intelligent and active food packaging materials.

scholarly journals Characterization and Antimicrobial Activity of Biodegradable Active Packaging Enriched with Clove and Thyme Essential Oil for Food Packaging Application

Foods ◽  
2020 ◽  
Vol 9 (8) ◽  
pp. 1117
Author(s):  
Shubham Sharma ◽  
Sandra Barkauskaite ◽  
Brendan Duffy ◽  
Amit K. Jaiswal ◽  
Swarna Jaiswal
Keyword(s):  
Antimicrobial Activity ◽  
Composite Film ◽  
Food Packaging ◽  
Antimicrobial Agents ◽  
Composite Films ◽  
Clove Oil ◽  
Thyme Oil ◽  
Uv Blocking ◽  
Control Film ◽  
Active Food Packaging

Bioactive packaging contains natural antimicrobial agents, which inhibit the growth of microorganisms and increase the food shelf life. Solvent casting method was used to prepare the Poly (lactide)-Poly (butylene adipate-co-terephthalate) (PLA-PBAT) film incorporated with the thyme oil and clove oil in various concentrations (1 wt%, 5 wt% and 10 wt%). The clove oil composite films depicted less green and more yellow as compared to thyme oil composite films. Clove oil composite film has shown an 80% increase in the UV blocking efficiency. The tensile strength (TS) of thyme oil and clove oil composite film decreases from 1.35 MPs (control film) to 0.96 MPa and 0.79, respectively. A complete killing of S. aureus that is a reduction from 6.5 log CFU/mL to 0 log CFU/mL was observed on the 10 wt% clove oil incorporated composite film. Clove oil and thyme oil composite film had inhibited E. coli biofilm by 93.43% and 82.30%, respectively. Clove oil composite film had exhibited UV blocking properties, strong antimicrobial activity and has high potential to be used as an active food packaging.

scholarly journals Aqueous Extract of Wild Cyclamen Tubers (Cyclamen Purpurascens L.) - A Potential Source of Natural Antioxidants and Antimicrobial Agents

2018 ◽  
Vol 16 (1-2) ◽  
Author(s):  
Ljiljana P. Stanojević ◽  
Milorad D. Cakić ◽  
Jelena S. Stanojević ◽  
Dragan J. Cvetković ◽  
Bojana R. Danilović
Keyword(s):  
Staphylococcus Aureus ◽  
Antimicrobial Activity ◽  
Aqueous Extract ◽  
Antimicrobial Agents ◽  
Natural Antioxidants ◽  
Diffusion Method ◽  
Total Phenolic ◽  
Dry Extract ◽  
Potential Source ◽  
Total Flavonoids Content

Wild cyclamen tubers (Cyclamen purpurascens Mill.) (mountain Kukavica, Southeast Serbia) were used as material for extraction in this study. Aqueous extract was obtained by reflux extraction on boiling temperature with hydromodulus 1:20 m/v during 180 minutes. The total phenolic content was determined spectrophotometrically by the method of Folin-Ciocalteu, and the total flavonoids content by method with AlCl3. The antioxidant activity of extract was investigated spectrophotometrically by DPPH and ABTS test. Disc-diffusion method was used for antimicrobial activity investigation on the following pathogenic microorganisms: Staphylococcus aureus, Bacillus cereus, Enterococcus faecalis, Pseudomonas aeruginosa and Klebsiella pneumoniae. The content of total phenols was 8.27 mg GAE/g dry extract while the total flavonoid content was 11.51 mg RE/g dry extract. The extract concentrations required to neutralize 50% of the initial concentration of DPPH radicals (EC50) after 20 minutes incubation and immediately after adding DPPH radical solution were 0.413 and 2.0 mg/ml, respectively, while concentrations of extract required to neutralize 50% of the initial ABTS radicals concentration is 0.743 mg/ml. The extract showed the highest antimicrobial activity on bacteria Staphylococcus aureus. The presented results indicate that cyclamen tubers extract is a potential source of natural antioxidants and antimicrobial agents.

Active food packaging films with synergistic antimicrobial activity

Food Control ◽  
2017 ◽  
Vol 76 ◽  
pp. 117-126 ◽  
Author(s):  
Maksym Krepker ◽  
Rotem Shemesh ◽  
Yael Danin Poleg ◽  
Yechezkel Kashi ◽  
Anita Vaxman ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Food Packaging ◽  
Packaging Films ◽  
Active Food Packaging ◽  
Food Packaging Films

scholarly journals Antioxidant and antimicrobial properties of ethanolic extracts of guarana, boldo, rosemary and cinnamon

2017 ◽  
Vol 20 (0) ◽  
Author(s):  
Jeannine Bonilla ◽  
Paulo Jose do Amaral Sobral
Keyword(s):  
Food Packaging ◽  
Phenolic Content ◽  
Antimicrobial Properties ◽  
Aureus Strain ◽  
Rosemary Extract ◽  
Total Phenolic ◽  
Food Industries ◽  
Cinnamon Extract ◽  
Active Food Packaging ◽  
Ethanolic Extracts

Abstract In this investigation, the ethanolic extracts of two less known plants, little reported in the literature (guarana and boldo leaves) were studied in comparison with the ethanolic extracts of two well studied plants (cinnamon and rosemary), regarding their colour, GC-MS profile, phenolic content and their antioxidant and antimicrobial properties. The rosemary (59.20 ± 0.28) and guarana (56.63 ± 0.54) extracts showed the highest values for luminosity (L*) and the UV-Vis absorption increased when L* decreased. GC-MS identified a limited number of compounds in the cinnamon and guarana extracts. The cinnamon extract showed the highest value for the total phenolic content (172 mg GA/g extract) as compared to the other extracts. The highest antioxidant capacity was observed for the boldo leaves extract in the TEAC (6.66 ± 0.17 mM) assay and for the rosemary extract in the DPPH (0.80 ± 0.14 mg/L) test. In addition, all the extracts showed antimicrobial activity against the S. aureus strain, indicating that all the extracts studied could be used by food industries to develop new active food packaging materials.

scholarly journals In-Vitro Studies on Antitumour and Antimicrobial Activities of Methanolic Kernel Extract of Mangifera Indica L. Cultivar Banganapalli

2019 ◽  
Vol 12 (1) ◽  
pp. 357-362 ◽  
Author(s):  
N. Venkata Raju ◽  
Karuganti Sukumar ◽  
G. Babul Reddy ◽  
P. K. Pankaj ◽  
Muralitharan G. ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Antimicrobial Activity ◽  
Bacillus Subtilis ◽  
Total Phenolic Content ◽  
Phenolic Content ◽  
Mangifera Indica ◽  
Antimicrobial Activities ◽  
Total Phenolic ◽  
Total Flavonoid Content ◽  
Flavonoid Content

Mangifera indica L. belongs to the family Anacardiaceae and is considered as “King of all Fruits”. Mango kernels are discarded as waste after the industrial processing and it has several proven medicinal benefits. Attempts were made to study its antitumour and antimicrobial activities. In the current research work, 15 local cultivars of Mangifera indica L. were collected with a motto to screen the best cultivar having high total phenolic content, flavonoid content, antitumour property and antimicrobial activity. Banganapalli cultivar of mango showed high total phenolic content and total flavonoid content i.e. 63.5±1.1 mg GAE/g and 16.7±0.5 mg quercetin/g followed by Royal special cultivar (TPC-58.7±0.6 mg GAE/g TFC-16.2±0.6 mg quercetin/g). Mangifera indica L. cultivar Banganapalli which showed highest total phenolic content and total flavonoid content was screened for its antitumour and antimicrobial properties. Antitumour property was tested by using potato disc assay which recorded 40.12% tumour inhibition percentage. Antimicrobial activity was assessed by agar diffusion method by taking 3 test microorganisms viz. Bacillus subtilis subsp. subtilis DSM 10, Staphylococcus aureus MTCC 737 and Escherichia coli MTCC 46. The measured area of inhibition is around Staphylococcus aureus MTCC 737 in 8.5±0.3 mm followed by E.coli MTCC 46 (8.2±0.3 mm) and Bacillus subtilis sub subtilis (6.6±0.5 mm). The present study showed that the mango kernels which were generally discarded as waste has antitumour and antibacterial properties and further studies need to be carried out.

scholarly journals Physical and Antioxidant Properties of Cassava Starch–Carboxymethyl Cellulose Incorporated with Quercetin and TBHQ as Active Food Packaging

Polymers ◽  
2020 ◽  
Vol 12 (2) ◽  
pp. 366 ◽  
Author(s):  
Wirongrong Tongdeesoontorn ◽  
Lisa J. Mauer ◽  
Sasitorn Wongruong ◽  
Pensiri Sriburi ◽  
Pornchai Rachtanapun
Keyword(s):  
Tensile Strength ◽  
Carboxymethyl Cellulose ◽  
Food Packaging ◽  
Water Solubility ◽  
Antioxidative Activity ◽  
Antioxidant Properties ◽  
Cassava Starch ◽  
Total Phenolic ◽  
Elongation At Break ◽  
Active Food Packaging

Antioxidant integration has been advocated for in polymer films, to exert their antioxidative effects in active packaging. In this study, the new antioxidant food packaging made from cassava starch–carboxymethyl cellulose (CMC), which is biodegradable, edible and inexpensive, was developed. Their properties were determined and applied in food models for application. Antioxidants (quercetin and tertiary butylhydroquinone (TBHQ)) were added at various concentrations into cassava starch–carboxymethyl cellulose (CMC) (7:3 w/w) films containing glycerol (30 g/100 g starch–CMC) as a plasticizer. The effects of quercetin and TBHQ concentrations on the mechanical properties, solubility, antioxidative activity, and applications of the films were investigated. Addition of antioxidant improved tensile strength, but reduced elongation at break of the cassava starch–CMC film. Cassava starch–CMC films containing quercetin showed higher tensile strength, but lower elongation at break, compared to films with TBHQ. Increases in quercetin and TBHQ content decreased water solubility in the films. Both the total phenolic content and antioxidative activity (DPPH scavenging assay) still remained in films during storage time (30 days). In application, cassava starch–CMC film containing quercetin and TBHQ can retard the oxidation of lard (35–70 days) and delay the discoloration of pork.

scholarly journals Electrospun Antimicrobial Films of Poly(3-hydroxybutyrate-co-3-hydroxyvalerate) Containing Eugenol Essential Oil Encapsulated in Mesoporous Silica Nanoparticles

Nanomaterials ◽  
2019 ◽  
Vol 9 (2) ◽  
pp. 227 ◽  
Author(s):  
Beatriz Melendez-Rodriguez ◽  
Kelly J. Figueroa-Lopez ◽  
Andrea Bernardos ◽  
Ramón Martínez-Máñez ◽  
Luis Cabedo ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Mesoporous Silica ◽  
Silica Nanoparticles ◽  
Food Packaging ◽  
Mesoporous Silica Nanoparticles ◽  
Life Extension ◽  
Release Capacity ◽  
Active Food Packaging ◽  
Closed Systems

The main goal of this study was to develop poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV) films with long-term antimicrobial capacity of interest in food packaging applications. To this end, eugenol was first highly efficiently encapsulated at 50 wt.-% in the pores of mesoporous silica nanoparticles by vapor adsorption. The eugenol-containing nanoparticles were then loaded in the 2.5–20 wt.-% range into PHBV by electrospinning and the resultant electrospun composite fibers were annealed at 155 °C to produce continuous films. The characterization showed that the PHBV films filled with mesoporous silica nanoparticles containing eugenol present sufficient thermal resistance and enhanced mechanical strength and barrier performance to water vapor and limonene. The antimicrobial activity of the films was also evaluated against foodborne bacteria for 15 days in open vs. closed conditions in order to simulate real packaging conditions. The electrospun PHBV films with loadings above 10 wt.-% of mesoporous silica nanoparticles containing eugenol successfully inhibited the bacterial growth, whereas the active films stored in hermetically closed systems increased their antimicrobial activity after 15 days due to the volatile portion accumulated in the system’s headspace and the sustained release capacity of the films. The resultant biopolymer films are, therefore, potential candidates to be applied in active food packaging applications to provide shelf life extension and food safety.

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