Probiotic Properties and Antioxidant Activities of Pediococcus pentosaceus SC28 and Levilactobacillus brevis KU15151 in Fermented Black Gamju

Black gamju is Korean traditional beverage fermented with molds. The aim of this study was to assess the probiotic properties and antioxidant activities of novel Pediococcus pentosaceus SC28 and Levilactobacillus brevis KU15151 to develop black gamju with bioactive properties for health. Tolerance against artificial gastric juice and bile salts, adhesion ability on HT-29 cells of strains, and antibiotics susceptibility were evaluated as probiotics, and various enzyme productions were detected. The 2,2-diphenyl-1-picrylhydrazyl assay, 2,2′-azinobis(3-ethylbenzothiazoline-6-sulfonate, and β-carotene bleaching assay were used for antioxidant activity of samples. The tolerance of both strains to artificial gastric juice and bile salts (Oxgall) was more than 90%. Additionally, both strains did not produce β-glucuronidase and were resistant to gentamicin, kanamycin, streptomycin, and ciprofloxacin. After fermentation of black gamju with each strain, the number of viable lactic acid bacteria increased to 8.25–8.95 log colony forming unit/mL, but the pH value of fermented samples decreased more (to pH 3.33–3.41) than that of control (pH 4.37). L. brevis KU15151 showed higher adhesion activity to HT-29 cells and antioxidant effects than P. pentosaceus SC28 in three antioxidant assays.

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In vitro assessment of the probiotic potential of lactobacilli isolated from Minas artisanal cheese produced in the Araxá region, Minas Gerais state, Brazil

Arquivo Brasileiro de Medicina Veterinária e Zootecnia ◽

10.1590/1678-4162-10188 ◽

2019 ◽

Vol 71 (2) ◽

pp. 647-657 ◽

Cited By ~ 1

Author(s):

J.G. Silva ◽

R.D. Castro ◽

F.M. Sant’Anna ◽

R.M. Barquete ◽

L.G. Oliveira ◽

...

Keyword(s):

Hydrogen Peroxide ◽

Gastric Juice ◽

Minas Gerais ◽

Starter Cultures ◽

Probiotic Properties ◽

Probiotic Potential ◽

Three Samples ◽

Production Of Hydrogen ◽

Artificial Gastric Juice

ABSTRACT Minas artisanal cheese is made from endogenous starter cultures, including lactic acid bacteria (LAB). Some LAB may possess probiotic potential. Thus, this study aimed to evaluate the in vitro probiotic properties of lactobacilli isolated from Minas artisanal cheeses produced in Minas Gerais. Ten samples of lactobacilli, formerly isolated from those cheeses, were submitted to the following assays: antimicrobial susceptibility, tolerance to artificial gastric juice and biliary salts, production of hydrogen peroxide and antagonism against pathogenic and non-pathogenic micro-organisms. Only L. plantarum (C0) was sensitive to all tested antimicrobials, while the other LAB samples were resistant to at least one drug. Six samples were tolerant to artificial gastric juice, and L. brevis (A6) even grew in that medium. Three samples were tolerant to biliary salts. Only L. brevis (E35) produced hydrogen peroxide. Difference (P< 0.05) was observed among the means of inhibition haloes of lactobacilli against Enterococcus faecalis ATCC 19433 and Lactobacillus plantarum C24 in spot-on-the-lawn assay. All samples of lactobacilli inhibited Escherichia coli ATCC 25922, Salmonella enterica var. Typhimurium ATCC 14028 in co-culture antagonism test (P< 0.0001). Most lactobacilli samples showed in vitro probiotic potential. From the tested samples, L. brevis (A6) presented the best results considering all in vitro probiotic tests.

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Characterization of Lactobacillus Isolates from Human Mouth and Feces as Probiotics

Cihan University-Erbil Scientific Journal ◽

10.24086/cuesj.v4n1y2020.pp7-12 ◽

2020 ◽

Vol 4 (1) ◽

pp. 7-12

Author(s):

Wala’a Sh. Ali ◽

Aya T. Reza

Keyword(s):

Bile Salts ◽

High Surface ◽

Lactobacillus Rhamnosus ◽

Cell Surface Hydrophobicity ◽

Surface Hydrophobicity ◽

The Other ◽

Human Beings ◽

Adhesion Ability ◽

Antibiotics Susceptibility

Probiotics are live microbes that give many health benefits to human beings and animals, the most studied and commonly used probiotics are Gram-positive bacteria; lactobacilli and bifidobacteria. At nowadays, Lactobacillus spp. constitute more than two-thirds of the total numbers of probiotic species. The present study aimed to characterize Lactobacillus that locally isolated from human mouth and feces as probiotics. A total of three Lactobacillus isolates; Lactobacillus fermentum Lb2, Lactobacillus rhamnosus Lb9, and Lactobacillus paracasei Lb10 were investigated in respect to acid and bile salts tolerance, antibiotics susceptibility, and cell surface hydrophobicity in vitro using bacterial adhesion to hydrocarbons method. In comparison with the other two isolates, the isolate L. fermentum Lb2 was able to grow in all pH values and in the presence of different concentrations of bile salts. Antibiotics susceptibility profile showed that the tested Lactobacillus isolates were sensitive to ampicillin, amoxicillin, and erythromycin, while they were resistant to the other antibiotics that used in this study. L. fermentum Lb2 exhibited high surface hydrophobicity (77.26%), while the other tested isolates; L. rhamnosus Lb9 and L. paracasei Lb10 revealed moderate adhesion abilities, 68.56% and 65%, respectively. L. fermentum Lb2 exhibited good probiotic behavior with respect to acid and bile salt tolerance as well as adhesion ability to hydrocarbons.

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Anti-Salmonella Potential of New Lactobacillus Strains with the Application in the Poultry Industry

Polish Journal of Microbiology ◽

10.33073/pjm-2020-001 ◽

2020 ◽

Vol 69 (1) ◽

pp. 5-18

Author(s):

JUSTYNA D. KOWALSKA ◽

ADRIANA NOWAK ◽

KATARZYNA ŚLIŻEWSKA ◽

MAŁGORZATA STAŃCZYK ◽

MAGDALENA ŁUKASIAK ◽

...

Keyword(s):

Antimicrobial Activity ◽

Bile Salts ◽

Antagonistic Activity ◽

Low Ph ◽

High Potential ◽

Probiotic Properties ◽

Bacterial Strains ◽

Slab Method ◽

Prevention And Treatment ◽

Antibiotics Susceptibility

Probiotics are considered an alternative to antibiotics in the prevention and treatment of Salmonella diseases in poultry. However, to use probiotics as proposed above, it is necessary to evaluate their properties in detail and to select the most effective bacterial strains in the application targeted. In this study, probiotic properties of new Lactobacillus sp. strains were investigated and their antimicrobial activity against 125 environmental strains of Salmonella sp. was determined using the agar slab method. Furthermore, their survival in the presence of bile salts and at low pH, antibiotics susceptibility, aggregation and coaggregation ability, adherence to polystyrene and Caco-2 cells, and cytotoxicity were investigated. Each strain tested showed antagonistic activity against at least 96% of the environmental Salmonella sp. strains and thus representing a highly epidemiologically differentiated collection of poultry isolates. In addition, the probiotic properties of new Lactobacillus strains are promising. Therefore, all strains examined showed a high potential for use in poultry against salmonellosis.

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Mucosa-Adherent Pediococcus Pentosaceus I44 Isolated From Healthy Human And Effect Of Oleic Acid On Its Probiotic Properties

Current Research in Microbial Sciences ◽

10.1016/j.crmicr.2021.100058 ◽

2021 ◽

pp. 100058

Author(s):

Lavanya Vasudevan ◽

Dr. V. Jayanthi ◽

Dr. M. Srinivas ◽

Dr. Chandra TS

Keyword(s):

Oleic Acid ◽

Probiotic Properties ◽

Healthy Human ◽

Pediococcus Pentosaceus

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The effects of gastrointestinal function on the incidence of ventilator-associated pneumonia in critically ill patients

Open Medicine ◽

10.1515/med-2018-0082 ◽

2018 ◽

Vol 13 (1) ◽

pp. 556-561

Author(s):

Yuanqiang Lin ◽

Zhixia Sun ◽

Hui Wang ◽

Meihan Liu

Keyword(s):

Gastric Juice ◽

Critically Ill ◽

Critically Ill Patients ◽

Ph Value ◽

Serum Levels ◽

Control Group ◽

Gastrointestinal Function ◽

Ventilator Associated Pneumonia ◽

Observation Group ◽

Gastric Feeding

AbstractObjectiveTo investigate the effect of gastrointestinal function on the incidence of ventilator-associated pneumonia (VAP) in critically ill patients.MethodsFrom August 2012 to June 2016, 160 critically ill patients in the ICU (Intensive Care Unit) of our hospital were selected as the research group; patients were divided equally into an observation group and a control group, 80 patients in each group, based on the random draw envelope principle. The control group was given a nasogastric tube for gastric feeding, the observation group was given a dual lumen gastrointestinal enteral device for gastric feeding; the two groups’ enteral nutrition observation time was 7d; any changes in patient condition and prognosis were recorded.ResultsThe pH value of gastric juice in the control group and the observation group was 6.13±1.38 and 4.01±1.83, respectively: the pH for the observation group was significantly lower than that of the control group (t=4.982, P<0.05). The incidence of VAP in the observation group and the control group was 2.5% and 12.5%, respectively: the VAP for the observation group was significantly lower than that of the control group (P<0.05). The serum levels of pre-albumin and albumin after feeding in the two groups were significantly higher than before feeding (P<0.05); the serum levels of pre-albumin and albumin in the observation group after feeding were significantly higher than those in the control group (P<0.05). The mechanical ventilation time and ICU length of stay in the observation group were 9.12±2.13 days and 12.76±1.98 days, respectively, significantly lower than those of the control group of 10.56±2.89 days and 16.33±2.11 days (P<0.05).ConclusionObstacles to gastrointestinal function in critically ill ICU patients are common; enteral gastric feeding by dual lumen gastrointestinal for can improve the patient’s nutritional status, promote and maintain the normal pH value of gastric juice, thereby reducing the incidence of VAP through rehabilitation of patients.

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Preliminary Characterization of the Probiotic Properties of a Bacterial Strain for Used in Monogastric Nutrition

Bulletin of University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca Animal Science and Biotechnologies ◽

10.15835/buasvmcn-asb:0015.19 ◽

2019 ◽

Vol 76 (2) ◽

pp. 102 ◽

Cited By ~ 1

Author(s):

Mihaela DUMITRU ◽

Mihaela HĂBEANU ◽

Cristina TABUC ◽

Ștefana JURCOANE

Keyword(s):

Bacillus Subtilis ◽

Bile Salts ◽

Good Growth ◽

Probiotic Properties ◽

Simulated Intestinal Fluid ◽

Phenotypic Profile ◽

Subtilis Atcc ◽

Bacillus Subtilis Atcc

This study aimed to evaluate some probiotic properties of Bacillus subtilis ATCC 6051a. The phenotypic profile, resistance to pH by simulated gastric juice (pH 2 and 3), bile salts by simulated intestinal fluid, survivability (%), heat and antibiotics tolerance were investigated. The strain is a Gram-positive, rod-shaped bacteria, arranged in short chains or in small irregular pairs with the ability to produce spores. Good viability at pH 2 and 3, with a survival of more than ≥80%, was found. In the presence of bile salts 0.3%, over 4 h, the strain exhibited a survival ≥85%. At 80°C, for 120 min., the strain showed good growth (9.04 log CFU/ml). Results were sensitive to most antibiotics, with a highly susceptible (between 16 – 25 mm) to erythromycin, clindamycin, amoxicillin, chloramphenicol, ciprofloxacin, amikacin and kanamycin. The strain was found to be sensitive to vancomycin, gentamicin, and tetracycline. The present research demonstrated that Bacillus subtilis ATCC 6051a can survive under gastrointestinal conditions, which involves them to future in vitro and in vivo probiotic studies.

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Adhesive and Chemokine Stimulatory Properties of Potentially Probiotic Lactobacillus Strains

Journal of Food Protection ◽

10.4315/0362-028x-70.1.125 ◽

2007 ◽

Vol 70 (1) ◽

pp. 125-134 ◽

Cited By ~ 43

Author(s):

MARÍA G. VIZOSO PINTO ◽

TOBIAS SCHUSTER ◽

KARLIS BRIVIBA ◽

BERNHARD WATZL ◽

WILHELM H. HOLZAPFEL ◽

...

Keyword(s):

Epithelial Cells ◽

Transforming Growth Factor ◽

Intestinal Epithelial Cells ◽

Probiotic Properties ◽

Intestinal Epithelial ◽

Chemokine Production ◽

Adhesion Ability ◽

Ht29 Cells ◽

Intestinal Pathogens ◽

Probiotic Strains

Five Lactobacillus plantarum strains and two Lactobacillus johnsonii strains, stemming either from African traditionally fermented milk products or children's feces, were investigated for probiotic properties in vitro. The relationship between the hydrophobic-hydrophilic cell surface and adhesion ability to HT29 intestinal epithelial cells was investigated, and results indicated that especially the L. johnsonii strains, which exhibited both hydrophobic and hydrophilic surface characteristics, adhered well to HT29 cells. Four L. plantarum and two L. johnsonii strains showed high adherence to HT29 cells, generally higher than that of the probiotic control strain Lactobacillus rhamnosus GG. Most strains with high adhesion ability also showed high autoaggregation ability. The two L. johnsonii strains coaggregated well with the intestinal pathogens Listeria monocytogenes Scott A, Staphylococcus aureus ATCC 25923, Escherichia coli ATCC 25922, and Salmonella enterica serovar Typhimurium ATCC 14028. The L. plantarum BFE 1685 and L. johnsonii 6128 strains furthermore inhibited the adhesion of at least two of these intestinal pathogens in coculture with HT29 cells in a strain-dependent way. These two potential probiotic strains also significantly increased interleukin-8 (IL-8) chemokine production by HT29 cells, although modulation of other cytokines, such as IL-1, IL-6, IL-10, monocyte chemoattractant protein-1 (MCP-1), tumor necrosis factor alpha (TNF-α), and transforming growth factor β (TGF-β), did not occur. Altogether, our results suggested that L. plantarum BFE 1685 and L. johnsonii BFE 6128 showed good adherence, coaggregated with pathogens, and stimulated chemokine production of intestinal epithelial cells, traits that may be considered promising for their development as probiotic strains.

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In vitro probiotic properties of Pediococcus pentosaceus L1 and its effects on enterotoxigenic Escherichia coli-induced inflammatory responses in porcine intestinal epithelial cells

Microbial Pathogenesis ◽

10.1016/j.micpath.2020.104163 ◽

2020 ◽

Vol 144 ◽

pp. 104163 ◽

Cited By ~ 1

Author(s):

Huajuan Yin ◽

Pengfei Ye ◽

Qingzhi Lei ◽

Yandong Cheng ◽

Hang Yu ◽

...

Keyword(s):

Escherichia Coli ◽

Epithelial Cells ◽

Inflammatory Responses ◽

Intestinal Epithelial Cells ◽

Enterotoxigenic Escherichia Coli ◽

Probiotic Properties ◽

Intestinal Epithelial ◽

Pediococcus Pentosaceus

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Development, characterization and antioxidant activity of polysorbate based O/W emulsion containing polyphenols derived from Hippophae rhamnoides and Cassia fistula

Brazilian Journal of Pharmaceutical Sciences ◽

10.1590/s1984-82502013000400016 ◽

2013 ◽

Vol 49 (4) ◽

pp. 763-773 ◽

Cited By ~ 6

Author(s):

Barkat Ali Khan ◽

Naveed Akhtar ◽

Haroon Khan ◽

Valdir de Andrade Braga

Keyword(s):

Antioxidant Activity ◽

Ph Value ◽

Antioxidant Activities ◽

Physical Stability ◽

Tween 80 ◽

Ionic Surfactant ◽

Cassia Fistula ◽

Ph Values ◽

Different Types ◽

Electrical Conductivity Data

The objective of this study was to develop a pharmaceutical O/W emulsion containing plant-derived polyphenol extracts and evaluate its stability and antioxidant activity. O/W emulsions were prepared using ionic surfactant polysorbate 80 (Tween 80®). The odorwas adjusted with few drops of blue sea fragrance. DPPH (1,1-diphenyl-2-picrylhydrazyl) assay was used to evaluate the antioxidant activity of the plant extracts alone and emulsions containing these extracts. Physical stability was assessed by submitting the emulsions to storage at 8 ºC, 25 ºC, 40 ºC and 40 ºC + 70% RH (relative humidity) for two months. Various physical characteristics of emulsions monitored, include color, creaming, liquefaction, centrifugation and pH. Brookfield rotational rheometer was used to determined viscosities and rheological behavior of emulsions. Different types of emulsion were determined microscopically, while pH values of emulsions were measured by a pH meter. Electrical conductivity data confirmed that the outer phase was water. Samples presented an acceptable pH value for an external topical use. Shear thinning behaviour was observed for all emulsions. The polyphenol-rich-plant-derived extracts alone and the extract containing emulsions showed good antioxidant activities. This research confirmed that the method used was suitable for preparing emulsions with Hippophae rhamnoids and Cassia fistula extracts, suggesting that those emulsions are suitable for topical use.

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