Bio-Remediation of Agro-Based Industries’ Wastewater and Mass Production of Spirulina (Spirulina platensis (Gomont) Geitler 1925)

2021 ◽  
Vol 3 (1) ◽  
pp. Vol.3
Author(s):  
Jerentulina Vijayarasa ◽  
Kandiah Pakeerathan ◽  
Nagarathnam Thiruchchelvan ◽  
Gunasingham Mikunthan

Spirulina (Spirulina platensis (Gomont) Geitler 1925) is a cyanobacteria used as an ecologically sound, nutrient-rich dietary supplement. These microalgae have the capability to produce the least-cost protein per unit area compared to livestock and are therefore being investigated to address malnutrition and food security. Apart from the variety of components, such as animal feed, fertilizer, and cosmetics, produced from Spirulina, phytoremediation of wastewater using Spirulina is an economically viable and environmentally sound tactic. A study was carried out with the objectives of the quick removal of waste from selected wastewaters produced by agro-based industries and the identification of suitable organic wastes as costless media for growing S. platensis for its powder production. Wastewater from fishponds and poultry units, grain-soaked water, and parboiled rice liquid waste were selected as treatments and inoculated with stock cultures of S. platensis. Treatments were replicated three times along with Zarrouk’s medium as standard control and arranged in a randomized complete block design. The chemical parameters of wastes, such as OD value, pH, and EC (mS/cm), and the growth of Spirulina were measured using a UH5300-Spectrophotometer with a wavelength of 560 nm at two-day intervals. The derived data were analyzed using SAS 9.4, and the significance of results among treatments was determined according to Duncan’s multiple range test, with p < 0.05. The results showed that poultry wastewater was a suitable medium for S. platensis growth, with a harvestable density of 0.8 at a very low concentration (25%) in 7 days compared to standard Zarrouk’s medium. The maximum and significant OD value of 1.313 was observed on day 15 in poultry wastewater and was non-significant among other treatments at p < 0.05. For fishpond wastewater, the maximum OD value of 0.567 was obtained on day 15. The pH value of poultry wastewater declined from 9.28 to 7.5 after 15 days. The EC values among the treatments were not significant. Among the selected liquid media, poultry wastewater promotes growth of S. platensis better than other locally available wastewaters tested. This experiment concludes that agro-based industries’ wastewater can be bio-remediated by Spirulina and that nutrient-enriched wastewater can be used for the mass culture of Spirulina without nutrient supplements.

2020 ◽  
Vol 23 (1) ◽  
pp. 47-58
Author(s):  
SS Tanu ◽  
P Biswas ◽  
S Ahmed ◽  
SC Samanta

A field experiment was conducted at Agronomy Field Laboratory, Patuakhali Science and Technology University, Dumki, Patuakhali from July 2018 to November 2018 to evaluate the effect of sunflower residues and herbicides on the yield and economic performance of transplanted Aman rice. Weed control methods tested were T1 = weedy check (Unweeded control), T2 = Weed-free check by hand weeding twice, T3 = Pendimethalin, T4 = Pretilachlor, T5 = Butachlor, T6 = Pyrazosulfuron ethyl, T7 = Bensulfuron methyl + Acetachlor, T8 = Bispyriback sodium, T9 = 2,4-D amine, T10 = MCPA, T11 = Sunflower residues, T12 = Sunflower residues + 100% Pyrazosulfuron ethyl, T13 = Sunflower residues + 75% Pyrazosulfuron ethyl, T14 = Sunflower residues + 50% Pyrazosulfuron ethyl. The experiment was laid out in a randomized complete block design with fourteen treatments replicated thrice. Weedy check registered significantly the highest total weed density (354.67 m-2) and total weed dry matter (51.81 g-2) while weed-free treatment by hand weeding twice recorded significantly the lowest total weed density (6.67 m-2) and total weed dry matter 0.49 g-2) . Weedy check produced the highest weed index (34.24%) and hand weeding produced the lowest. Among different herbicides applied alone, butachlor had the lowest total weed density (15 m-2) and total weed dry matter (6.43 g-2) after hand weeding. Hand weeding recorded the highest grain yield (5.14 t ha-1) which was statistically similar to pendimethalin, pretilachlor, butachlor, bensulfuron methyl + acetachlor and sunflower residues + 100% pyrazosulfuron ethyl. Higher grain yield was attributed to a higher number of panicle m-2, number of filled grains panicle-1 and 1000-grain weight. The highest gross margin (22955 Tk. ha-1) and benefit-cost ratio (1.32) were obtained from butachlor. Integration of sunflower residues with pyrazosulfuron ethyl produced effective weed suppression and satisfactory yield comparable to butachlor. Although the integration is less profitable than butachlor the farmers can use this technology as a feasible and environmentally sound approach in transplanted Aman rice field. Bangladesh Agron. J. 2020, 23(1): 47-58


2021 ◽  
Vol 99 (Supplement_1) ◽  
pp. 201-201
Author(s):  
Sudario Roberto Silva Junior ◽  
Rhuan F Chaves ◽  
Maíra Resende ◽  
Ygor Henrique De Paula ◽  
Kenio De Gouvêa Cabral ◽  
...  

Abstract Antibiotics growth promoting (AGP) in animal feed has been restricted. With this, several additives have been tested in order to replace AGP and ensure intestinal health. The objective of this study was to evaluate the effects of a blend of additives on growth performance, diarrhea incidence and cecal content microbiota of nursery pigs. A total of 576 weaned pigs (6.21 ± 1.16 kg) were assigned into 4 treatments (12 replicates) in randomized block design: basal diet without additives (NC); basal diet with 120 ppm of halquinol (PC); NC + 0,05% blend of lactic acid (3,5%), benzoic acid (2,5%), flavonoids (3,5%) (B5); NC+ 0,1% of same blend (B10). The pigs were weighed 0 and 42 days into the experiment. On day 10, one animal per pen (10 replicates) was euthanized for microbiota analyses. The diarrhea incidence was calculated by the percentage of visual pens with diarrhea, by day, in the period. The growth performance was analyzed by GLIMMIX procedure and the means were compared with Tukey test (P &lt; 0.050) (SAS, 2009). Diarrhea incidence was analyzed by GENMOD procedure (SAS, 2009). The Kruskal Wallis test was used to compare microbial biodiversity (P &lt; 0.050) by statistical program STAMP. The final weight (P = 0.010) and average daily gain (P = 0.001) of B10 and PC were higher than NC. Regarding diarrhea, it was observed that the B5 and B10 had lower incidence when compared to other groups in the total period. In the microbiome analyses, it was observed that the B5, B10, and PC groups had lower (P = 0.021) biodiversity when compared to the NC piglets. However, the B5 and B10 group had higher biodiversity when compared to the PC group. In conclusion, the supplementation with 0,1% blend of additives can be a substitute for AGP.


2021 ◽  
Vol 6 (1) ◽  
pp. 23-26
Author(s):  
Maryana Mohamad Nor ◽  
Lukman Ismail ◽  
Siti Nuurul Huda Mohammad Azmin ◽  
Ikhmal Hisham Abdul Halim

Phoenic dactylifera (date) is a species of flowering plants in the family of Arecaceae. Date seeds are considered as a waste from many processing that produced plants pitted date, confectionery date syrup and date itself. Currently, the seeds are used mainly for animal feed in the cattle and chicken industry. In 2004, about 863,000 tonnes of date seeds are produced out of 6.9 million tonnes date. Tenderness is the major concern that affecting consumer acceptance of beef in meat industry. This study was carried out in order to investigate the effects of the bioactive compound extracted from date seed as a tenderizing agent in meat. Extraction of date seeds used a different method of extraction (Soxhlet and Maceration). The application of extracted on knuckle part of beef were performed and papain was used as a positive control and followed by the sensory evaluation. The analysis of cooked meat was performed in order to analyze the physico-chemical properties of date seeds extract. The result from the study revealed that the aqueous extract (maceration techniques) gave the best percentage of the total yield recovery with 28.44%. The physico-chemical properties of cooked meat showed the reducing of pH value after cooking. Meanwhile for the cooking yield, result showed that almost 86% of water losses during cooking for aqueous extract and positive control and 96% for negative control. According to the sensory evaluation of the cooked meat, scoring test and hedonic test were performed using One Way Anova. The result for texture is 6.10±2.1, juiceness is 5.87±1.76 and taste is 6.80±1.34. All attributes have no significant different at p <0.05 between aqueos extract, and positive control. A general acceptance shows that no significant different between aqueos extract (6.50±2.0) and positive control (7.13±1.98). The result suggested that the tenderization effect of date seed improved the textural properties of knuckle part meat and have potential for tenderization purpose in food industry.


2011 ◽  
Vol 1 (1) ◽  
pp. 38 ◽  
Author(s):  
Nita Noriko

Tofu industry produce organic waste in the form of liquid and solid. But, most of the industry cast away its waste to the surroundings directly without any processes before and contaminate the environment. One way out to solve this problem is by functionalizing the liquid waste as growth media of Spirulina platensis. In this research, exemic culture method with independent microalgae is used. Based on the result of the research, the optimal  growth of Spirulina platensis  if  cultured in 10% concentration of liquid waste with 4:6 comparison (400 ml Spirulina platensis : 600 ml tofu liquid waste). It is indicated by green color intensity of Spirulina platensis.


2020 ◽  
Vol 36 (4) ◽  
Author(s):  
Natália Silva Assunção ◽  
Natalia Oliveira Silva ◽  
Flávio Lemes Fernandes ◽  
Leonardo Angelo de Aquino ◽  
Maria Elisa De Sena Fernandes

The objective of this study was to evaluate the effect of nitrogen sources and rates on the physicochemical characteristics and yield of tomato plants. Forty hybrids were cultivated at 100 and 400 kg ha-1 of N, combined with four sources (urea, ammonium sulfate, ammonium nitrate and calcium), plus a treatment without N application in a randomized complete block design four replicates. Size, stem diameter, number of leaves, SPAD (Soil Plant Analysis Development), leaf nitrogen, number of fruits / plants, fruit firmness, bark thickness and average fruit size, ° BRIX, pH, calcium, potassium and sodium in fruits. There was an increase in SPAD index, Brix and longitudinal diameter of fruits as a function of the N dose. The use of ammonium nitrate and calcium provided stronger fruits. Urea and ammonium nitrate provided the highest pH value in tomato fruits. The application of the 100 kg ha-1 dose of N resulted in the highest potassium content in fruits. The highest productivity was obtained with the application of sources containing ammonium and the lowest in the control treatment. Sources and doses of nitrogen fertilizers influenced growth, productivity and parameters related to tomato quality.  


2017 ◽  
Vol 28 (2) ◽  
pp. 523
Author(s):  
Juan Sierra Márquez ◽  
Lucellys Sierra Márquez ◽  
Jesus Olivero-Verbel

The objective of this research was to highlight the economic importance of the optimal use of products and byproducts of oil palm. In Colombia, productive crops per hectare can generate, over one year, an average of 3.14 tons of oil, and up to 21.68 t of solid and liquid waste when the plant is on a productive stage. These data allowed the researcher to estimate that more than nine million of t of solid and liquid waste was produced from the 450 131 ha present in 2014, in Colombia; the produced biomass was used to generate energy and steam, releasing carbon dioxide back again into the environment. These residues have great potential in many industries, some to be developed, therefore, it is of special importance to try to maximize the use of waste produced by oil palm production, to generate economic and environmental benefits. An example of this is the palm kernel cake, with a nutritional potential in animal feed, fiber in the biocompound industry, biomass and stipe in the timber industry, glycerol, biodiesel, and liquid effluents in the chemical and biotechnological industry. The use of these raw materials may help to establish a positive balance in the cultivation of this species of oil palm in the country. 


Moringa is a typical representative of multi-purpose tropical tree crop due to the high nutritional value, it is an important source for food to many communities and provides raw materials for animal feed industries. This study investigates the influence of plant densities (15 x 15 cm, 15 x 20 cm, 20 x 20 cm, and 20 x 30 cm) and four (4) harvesting intervals (HI) of 2, 4, 6 and 8 weeks was laid out using Randomized Complete Block Design (RCBD) replicated three times. Data on silvicultural practices were collected and six (6) plants were randomly selected for harvest from each plot which were subsequently separated into leaves, stems and twigs. Fresh and air-dried weights were recorded for analysis. Analysis of Variance (ANOVA) was used for data analysis Duncan’s Multiple Range Test was used to separate the means. Results of the present study show non-significant effects of plant density (p>0.05) between treatments. However, greater number of branches, leaves, height and biomass accumulation was recorded at 8 weeks harvesting interval. A significant interaction effects were recorded between spacing and HI.


2021 ◽  
Vol 99 (Supplement_3) ◽  
pp. 386-387
Author(s):  
Yuli Andrea Pena Bermudez ◽  
Richard Roberto Lobo ◽  
Danny Alexander Rojas Moreno ◽  
Rafaela Scalise Xavier de Freitas ◽  
Ives Claudio C Da Silva Bueno

Abstract The presence of secondary metabolites in the structure of natural antioxidants makes them an alternative for animal feed, which allows modifying the composition of the meat, thus contributing to people’s health. For this reason, the aim of the present study was to evaluate the effects of yerba mate extract (YME) on lamb feed as a natural antioxidant in the fatty acid profile and physical traits of meat of 36 lambs fed for 53 days, fed with corn silage and concentrate in the proportion 40:60, respectively. The treatments followed the randomized block design, with a control group without YME (0%) and three groups with 1, 2, and 4% of YME inclusion in the dry matter (DM). Samples were collected from the Longissimus thoracis (LT), muscle for quality analysis and composition. All data were analyzed using a MIXED model with orthogonal contrasts. We found that the inclusion of YME did not affect significantly the characteristics of the pH, total lipids and color general (P = 0.192), although the use of YME don’t affect the parameters normal in the meat. Nevertheless, the use of YME improved the value of b* (P = 0.004), showing a yellowness coloration in the meat of the lambs. Likewise, changed the proportion of branched-chain fatty acids (BCFA; P = 0.009) and the conjugated linoleic acid (CLA; P = 0.030) exhibited a diet effect for the group with 4% YME. This is revealing result, since these fatty acids have been associated with a decrease in cardiovascular diseases. Therefore, the addition of up to 4% YME improves the composition and general acceptance of lamb meat, recommended for use in animal feed.


2021 ◽  
Vol 3 (1) ◽  
pp. 22
Author(s):  
Pubudu Kumara ◽  
Kandiah Pakeerathan ◽  
Liyanage P. P. Deepani

Green gram (Vigna radiata (L.) R. Wilczek) is one of the most economically important grain legumes of the traditional farming systems of Sri Lanka because it is a cheap source of protein and animal feed, and sustains soil fertility by fixing atmospheric nitrogen. Weeds are one of the major problems in green gram cultivation, reducing the yield through competition, interference with harvest and harboring pests and diseases. Controlling of weeds by applying herbicides would definitely cause unexpected damage to human health and the abundant biodiversity of Sri Lanka. Therefore, an investigation was planned to evaluate the yield loss due to weeds and to determine the optimum weed free period to minimize the yield losses. Two experiments were performed. The first experiment was conducted to determine the effects of different weed functional groups on the yield of green gram. In the second experiment, weeds were continuously hand weeded and areas kept weed free. In the third, weeds were allowed to compete with green gram until 2, 3, 4, 5 or 6 weeks after cultivation. All the treatments were conducted in randomized complete block design with three replicates. The data collected on types of weed, number of pods and pod weight at 3–6 weeks after planting (WAP) were analyzed using the SAS 9.4 statistical package, and DMRT was performed to determine the best treatment combination. The results from the first experiment showed that average yield loss due to total weed populations was 54.77%. Yield loss due to grasses alone was 46.56%, far worse than broad leaves (16.49%) and sedges (18.01%) at p < 0.05. Crop stand count at 3–4 WAP was not significantly different among treatments. However, biomass weight of 50 plants, number of pods in 50 plants and grain weight of 10 plants were found to be significantly different after 3–4 WAP in weed free conditions at p < 0.05%. When the weed free period increased, the yield was increased until 3 WAP; thereafter, not significant yield increment was observed. In contrast, yield steeply declined in plots that had weeds until 3 WAP. According to the results of the present study, it can be concluded that the critical weed free period from the planting of green gram is 3 WAP. Maintaining a weed free period for 3–4 weeks is recommended to minimize the yield loss of green gram at minimal weed management cost.


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