scholarly journals Response of Scenedesmus quadricauda (Chlorophyceae) to Salt Stress Considering Nutrient Enrichment and Intracellular Proline Accumulation

Author(s):  
Myung-Hwan Park ◽  
Chae-Hong Park ◽  
Yeon Bo Sim ◽  
Soon-Jin Hwang

Aquatic organisms are exposed to a wide range of salinity, which could critically affect their survival and growth. However, their survival and growth response to salinity stress remain unclear. This study evaluates the growth response and intracellular proline accumulation of green algae, Scenedesmus quadricauda, isolated from brackish water, against dissolved salts stress with N and P enrichment. We tested a hypothesis that nutrient enrichment can relieve the dissolved salts stress of algae by accumulating intracellular proline, thereby improving survival and growth. Four levels of salinity (0, 3, 6, 12 psu) were experimentally manipulated with four levels of nutrient stoichiometry (N:P ratio = 2, 5, 10, 20) at constant N (1 mgN/L) or P levels (0.05 and 0.5 mgP/L). In each set of experiments, growth rate and intracellular proline content were measured in triplicate. The highest level of salinity inhibited the growth rate of S. quadricauda, regardless of the nutrient levels. However, with nutrient enrichment, the alga showed tolerance to dissolved salts, reflecting intracellular proline synthesis. Proline accumulation was most prominent at the highest salinity level, and its maximum value appeared at the highest N:P ratio (i.e., highest N level) in all salinity treatments, regardless of P levels. Therefore, the effects of P and N on algal response to salt stress differ.

2021 ◽  
Vol 22 (2) ◽  
pp. 130-138
Author(s):  
T. Oguntona ◽  
R. Heale

A total of four experiments have been conducted to evaluate performance of chicks given high (300/kg diet) n-alkane grown yeast diet supplemented with vitamin E and selenium. Four levels (0,10,20,30 mg) of vitamin E were examined in experiment 1 while five levels (0,12.5,15.0,17.5,20mg) were examined in experiment 2. Performance was also measured at four selenium supplementation levels (0, 50,100, 150 µg Se/kg diet) initially and finally at five other (0,100, 125, 150, 175 µg/kg diet) more detailed levels. Supplementation of a 300g/kg yeast diet with 15mg Vitamin E produced significantly (P < 0.05) higher body weight than unsupplemented high yeast diet. Higher levels produced no further growth response. At this level of vitamin E supplementation every level of selenium examined gave significant (P < 0.05) increase in weight but optimum growth response was obtained at 100µg Se/kg yeast diet. There were no significant treatment effects on feed intake at all levels of vitamin E and selenium examined. Nitrogen retention also showed no response at the highest levels (100 - 157µg) of selenium supplementation. The  results show that supplementation with appropriate levels of vitamin E and selenium significantly reduced the depression in growth rate caused by high levels of inclusion of n-alkane grown yeast in chick diet.


2009 ◽  
Vol 8 (1) ◽  
pp. 11
Author(s):  
. Chumaidi ◽  
. Nurhidayat ◽  
A. Priyadi

<p>The enrichment of natural live  food with unsaturated fatty acid can increase survival and growth rate of fish larvae. The purpose of  this experiment was to know the effect of various natural live food with or without nutrient enrichment on survival and growth rate of clown loach (<em>Chromobotia macracanthus</em>) larvae.  The experiment was done indoor by using plastic jars filled 5 L water and aerated continuously.  Twenty clown loach larvae of four days old, 5.58 ± 0.12   mm in average length,  were stocked in plastic jars and  were reared for 28 days. The treatment were: a) <em>Artemia</em> nauplii as control;  b) rotifer without enrichment;  c) enriched rotifer, d) <em>Moina</em> nauplii without enrichment, and e) enriched <em>Moina</em> nauplii.  As enrichment agent, we used 200 mg of tuna eggs powder and 200 mg of baker's yeast which dissolved and  aerated in 2 L of water for one hour.  Proximate analysis for amino  and fatty acids content were done before the treatment, at 5 days old and at 32 days old (juvenile).  The result showed that higher survival (80.00%) and growth rate (average body length 12.80±1.85 mm) could be achieved by feeding clown loach larvae with enriched <em>Moina</em> nauplii.</p> <p>Keywords: clown loach larvae,  live feed, nutrient enrichment</p> <p> </p> <p>ABSTRAK</p> <p>Pengkayaan asam lemak tak jenuh dari pakan hidup dapat meningkatkan sintasan dan pertumbuhan larva ikan.  Penelitian dilakukan bertujuan untuk mengetahui pengaruh pakan hidup dengan atau  tanpa diperkaya nutrisinya terhadap sintasan dan pertumbuhan larva ikan botia (<em>Chromobotia macracanthus</em>).  Penelitian dilaksanakan di dalam ruangan tertutup menggunakan wadah plastik yang berisi media air 5 L serta diaerasi terus menerus.  Larva umur empat hari dengan panjang rata-rata 5,58 ± 0,12 mm ditebar sebanyak 20 ekor per wadah.  Pemeliharaan larva dilakukan selama 28 hari. Perlakuan perbedaan jenis pakan hidup dengan atau tanpa diperkaya, yaitu : a) naplii<em> Artemia</em> (kontrol), b) rotifer tanpa diperkaya nutrisinya, c) rotifer diperkaya nutrisinya, d) nauplii <em>Moina</em> tanpa diperkaya nutrisinya dan e) nauplii<em> Moina</em> diperkaya nutrisinya.  Pengkayaan pakan alami menggunakan tepung telur tuna dan ragi roti masing-masing 200 mg dalam 2 L media air yang diaerasi selama 1 jam. Analisis proksimat, asam amino dan asam lemak pakan alami  dan larva umur empat hari dilakukan sebelum penelitian dan juvenil botia 32 hari dianalisis pula.  Hasil penelitian menunjukkan bahwa sintasan (80,00%) dan pertumbuhan (panjang mutlak 12,90 ± 1,85 mm) yang tinggi dapat diperoleh dengan memberikan nauplii <em>Moina</em> yang diperkaya pada larva botia</p> <p>Kata kunci: larva ikan botia, pakan alami, pengkayaan nutrisi</p>


2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Xue-Ming Zai ◽  
Jun-Jun Fan ◽  
Zhen-Ping Hao ◽  
Xing-Man Liu ◽  
Wang-Xiang Zhang

AbstractBeach plum (Prunus maritima) is an ornamental plant, famous for its strong salt and drought stress tolerance. However, the poor growth rate of transplanted seedlings has seriously restricted its application in salinized soil. This study investigated the effects of inoculation with arbuscular mycorrhizal fungus (AMF), Funneliformis mosseae, and phosphate-solubilizing fungus (PSF), Apophysomyces spartima, on the growth, nutrient (N, P, and K) uptake, and photosynthesis of beach plum under saline (170 mM NaCl) and non-saline (0 mM NaCl) conditions. We aimed to find measures to increase the growth rate of beach plum in saline-alkali land and to understand the reasons for this increase. The results showed that salinization adversely affected colonization by AMF but positively increased PSF populations (increased by 33.9–93.3% over non-NaCl treatment). The dual application of AMF and PSF mitigated the effects of salt stress on all growth parameters and nutrient uptake, significantly for roots (dry weight and P and N contents increased by 91.0%, 68.9%, and 40%, respectively, over non-NaCl treatment). Salinization caused significant reductions in net photosynthetic rate (Pn), stomatal conductance (Gs), transpiration rate (E), and intercellular CO2 concentration (Ci) value, while inoculation with AMF and PSF inoculations significantly abated such reductions. The maximum efficiency of photosystem II (PSII) (Fv/Fm), the photochemical quenching coefficient (qP), and the nonphotochemical quenching (NPQ) values were affected little by inoculation with AMF, PSF, or both under non-NaCl treatments. However, plants inoculated with AMF and/or PSF had higher Fv/Fm, qP, and ФPSII values (increased by 72.5–188.1%) than the control under NaCl treatment, but not a higher NPQ value. We concluded that inoculation with AMF or PSF increased nutrient uptake and improved the gas-exchange and Chl fluorescence parameters of beach plum under salt stress environment. These effects could be strengthened by the combination of AMF and PSF, especially for nutrient uptake, root growth, and Pn, thereby alleviating the deleterious effects of NaCl stress on beach plum growth.


2021 ◽  
Vol 13 (1(22)) ◽  
pp. 34-42
Author(s):  
Petru Cuza ◽  
◽  
Corina Certan ◽  
Constantin Bulimaga ◽  
◽  
...  

The rooting, the survival and dynamics of growths in the height and after the diameter of the seedlings, of a range of woody species, had investigated on the freshly formed sterile dump limestone quarry „Lafarge Ciment”. The seedlings of the woody species had a relatively good rooting (69-99%). A high degree of rooting can be ensured as a result of the maintenance of forest crops. After planting, the young seedlings must be cared for by hoeing as many times as necessary in order to control the weeds and maintain moisture in the soil, which increases the survival of the seedlings. Tree species have been characterized by a different growth rate. Acacia has showed a very rapid increase in height, but the black pine has been characterized by a slow growth. Acacia being a fast-growing species and unpretentious to the mineral elements in the soil can be used to afforest the limestone quarry land. In the fi rst year after planting, the young seedlings, otherwise sensitive to the action of the environmental factors, had an increase in height and in diameter slow and uneven. In the years that followed, the seedlings became more viable and less infl uenced by local and temporal fl uctuations of environmental factors.


Jurnal Wasian ◽  
2017 ◽  
Vol 4 (2) ◽  
pp. 69
Author(s):  
Ady Suryawan

This study aimed to know the dynamic of survival rate and growth of Rhizophora mucronata Lamk which planted by using propagules at Alo Beach, Karakelang Island, Talaud. The study was conducted from June 2013 to April 2017, used a complete randomized design with 6 treatments i.e. K1, K2, K3, K4, K5, K6. The amount of propagules in every treatment were 100 pieces each. The parameter observed were characteristic of substrat, survival rate,height and diameter of the plant’s in age of 1.5 months and 48 months. The results showed that treatment had a significant effect on survival rate and growth of Rhizophora mucronata plants. The rehabilitation was categorized as success at 1.5 months old, but it was failed inthe 48 months. Beside waves and tides, low substrat nutrition became the main factor that affect the success of mangrove rehabilitation in Alo Beach.The K5 treatment has the highest survival and growth rate of the plant. The K5 treatment using pnematophore as brace, while planting without brace/K1 has lowest survival and growth rate since months old.Keywords: mangroves, rehabilitation, Rhizophora mucronata, Talaud


1976 ◽  
Vol 24 (6) ◽  
pp. 681 ◽  
Author(s):  
RH Groves ◽  
K Keraitis

Seedlings of Banksia serrata, Acacia suaveolens and Eucalyptus pilularis were grown in sand culture for 3-4 months at four levels of phosphorus (0, 5, 50, and 100 ppm) and three of nitrogen (0, 25 and 250 ppm) applied gradually in all combinations. B.serrata died at high phosphorus-high nitrogen levels, A.suaveolens died with high phosphorus irrespective of the nitrogen level, and E. pilularis did not survive high phosphorus or high nitrogen levels. There were differences between species in their growth responses to increasing levels of phosphorus and nitrogen. Dry weights of seedlings were greatest at P5N25 for B.serrata, at P5N250 for A.suaveolens, and at P5N250 for E.pilularis. Shoot phosphorus concentrations greater than 1% were directly associated with 'toxicity' and death of A.suaveolens seedlings only, and not of the other two species. The species differ in the weights and nutrient contents of their seeds, and this is discussed in relation to the different responses obtained. Growth of sclerophyll species on areas subject to disturbance (e.g. sand-mining) will be determined in large part by the levels of phosphorus and nitrogen applied and the rate at which nutrients are leached from the rooting zone.


2004 ◽  
Vol 34 ◽  
pp. 57-66 ◽  
Author(s):  
A. Yilmaz ◽  
M. Ozcan ◽  
B. Ekiz ◽  
A. Ceyhan ◽  
A. Altine

SummaryThis study was carried out to investigate the fertility, milk production and body weight of ewes and the survival and growth characteristics of lambs of the indigenous Imroz and Kivircik sheep breeds. The Imroz and Kivircik sheep breeds are included among the genetic resources in the project for the conservation of the indigenous breeds in Turkey.Conception rates for Imroz and Kivircik ewes were 57.6% and 81.7% respectively, litter sizes were 1.2 and 1.2 respectively, milk yields were 89 kg and 62 kg respectively and average live weights (2–7 years of age) were 43 kg and 55 kg, respectively. Imroz and Kivircik lambs had survival rates at weaning (90th day) of 98% and 96%, birth weights of 3.3 kg and 4.1 kg and weaning weights of 19.8 kg and 28.0 kg, respectively.The results showed that the Kivircik sheep were heavier and the lambs had a better growth rate until weaning than the Imroz breed. However, the milk production of the Imroz ewes was higher than that of Kivircik. The lambs of both breeds had a satisfactory survival and growth rates until weaning and these results might be regarded as a desired adaptation of the Imroz and Kivircik lambs to the environmental conditions of the Marmara Region.


1959 ◽  
Vol 53 (1) ◽  
pp. 78-101 ◽  
Author(s):  
A. J. Brookes ◽  
John Hodges

1. The sources of calves for rearing for beef are discussed and the dependence of the beef industry upon the dairy industry demonstrated.2. An experiment designed to compare the effects of four levels of feeding on the growth rate, conformation, economy of food conversion and type of carcase produced, of three breeds of spring-born steers, Hereford, Dairy Shorthorn and Friesian, is described. The levels of feeding are high or moderate in the calf stage, each followed by high or moderate feeding in subsequent winters.3. The results on growth and time of fattening are given and discussed.4. The calves fed on a high level were almost twice the weight of those fed on a moderate level at 8 months old.5. The well-reared calf had an advantage over the moderately reared calf for the rest of its life.6. Although calves which had been reared moderately showed a marked improvement in growth rate when subsequently fed on a higher level they did not reach the weight of calves fed well throughout at 2 years old.7. The level of feeding in the calf stage had little effect upon the time of fattening provided that the level of feeding in the winter periods of subsequent life was on a high level.8. When subsequent feeding was on a moderate level in the winter months, the calf reared well initially fattened on grass a year earlier than the moderately reared animal.9. The high-moderate treatment is shown to be the most satisfactory from many points of view. On this treatment a minimum of concentrates is used in the production of beef.10. The moderate-moderate cattle may be too heavy for present-day requirements when finished off grass at 3½ years old.11. Cattle which were fed on a high level in winter from 8 to 14 months put on less live weight in the following summer than those fed moderately. A live-weight gain of about 1 lb. a day in winter would appear to be satisfactory if the cattle are to make good use of grass in the following summer.12. These main feeding treatment effects applied to the three breeds used.13. The Friesians were heavier than Dairy Shorthorns or Herefords as calves, and the live weight at most ages was in descending order Friesians, Dairy Shorthorns, Herefords.14. The growth rate of Friesian steers was higher than that of the other two breeds at most stages of life, but the difference between Dairy Shorthorns and Herefords was negligible.15. The Herefords finished earlier than the Dairy Shorthorns and Friesians when fattened in yards or on grass.16. There was some indication (Brookes, 1954) that husk and ringworm caused less trouble with cattle which had been well reared than with those moderately reared.


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